def build_v1_model(r, lw_name):
plate = load(r, lw_name or '96-flat', '1')
tiprack = load(r, 'opentrons-tiprack-300ul', '2')
pipette = Pipette(r,
ul_per_mm=18.5, max_volume=300, mount='right',
tip_racks=[tiprack])
instrument = models.Instrument(pipette)
container = models.Container(plate)
return namedtuple('model', 'robot instrument container')(
robot=r,
instrument=instrument,
container=container,
)
def model(robot, hardware, loop, request):
# Use with pytest.mark.parametrize(’labware’, [some-labware-name])
# to have a different labware loaded as .container. If not passed,
# defaults to the version-appropriate way to do 96 flat
from opentrons.legacy_api.containers import load
from opentrons.legacy_api.instruments.pipette import Pipette
try:
lw_name = request.getfixturevalue('labware')
except Exception:
lw_name = None
if isinstance(hardware, hc.HardwareAPILike):
ctx = ProtocolContext(loop=loop, hardware=hardware)
pip = ctx.load_instrument('p300_single', 'right')
loop.run_until_complete(
hardware.cache_instruments({Mount.RIGHT: 'p300_single'}))
instrument = models.Instrument(pip, context=ctx)
plate = ctx.load_labware(lw_name or 'corning_96_wellplate_360ul_flat',
1)
rob = hardware
container = models.Container(plate, context=ctx)
else:
print("hardware is {}".format(hardware))
pipette = Pipette(robot, ul_per_mm=18.5, max_volume=300, mount='right')
plate = load(robot, lw_name or '96-flat', '1')
rob = robot
instrument = models.Instrument(pipette)
container = models.Container(plate)
return namedtuple('model',
'robot instrument container')(robot=rob,
instrument=instrument,
container=container)
def model_with_trough(robot):
from opentrons.legacy_api.containers import load
from opentrons.legacy_api.instruments.pipette import Pipette
pipette = Pipette(robot, ul_per_mm=18.5, max_volume=300, mount='right')
plate = load(robot, 'trough-12row', '1')
instrument = models.Instrument(pipette)
container = models.Container(plate)
return namedtuple('model',
'robot instrument container')(robot=robot,
instrument=instrument,
container=container)
def test_serial_dilution(robot):
plate = load(robot, '96-flat', '2', 'plate')
tiprack = load(
robot,
'tiprack-200ul', # container type from library
'1', # slot on deck
'tiprack' # calibration reference for 1.2 compatibility
)
trough = load(robot, 'trough-12row', '5', 'trough')
trash = load(robot, 'point', '3', 'trash')
p200 = pipette.Pipette(
robot,
ul_per_mm=18.5,
trash_container=trash,
tip_racks=[tiprack],
min_volume=10,
max_volume=200, # These are variable
mount='left',
channels=1)
p200.calibrate_plunger(top=0, bottom=10, blow_out=12, drop_tip=13)
for t, col in enumerate(plate.cols):
p200.pick_up_tip(tiprack[t])
p200.aspirate(10, trough[t])
p200.dispense(10, col[0])
for well, next_well in zip(col[:-1], col[1:]):
p200.aspirate(10, well)
p200.dispense(10, next_well)
p200.mix(repetitions=3, volume=10, location=next_well)
p200.drop_tip(trash)
def plate(robot):
return load(robot, '96-flat', '4')