def project_fmri_from_kernels(input_mesh, kernels_file, fmri_data_file,
output_tex, bin_threshold=None, ):
pyhrf.verbose(2,'Project data onto mesh using kernels ...')
if 0:
print 'Projecting ...'
print 'func data:', fmri_data_file
print 'Mesh file:', input_mesh
print 'Save as:', output_tex
pyhrf.verbose(2,'Call AimsFunctionProjection -op 1 ...')
data_files = []
output_texs = []
p_ids = None
if bin_threshold is not None:
d,h = read_volume(fmri_data_file)
if np.allclose(d.astype(int), d):
tmp_dir = pyhrf.get_tmp_path()
p_ids = np.unique(d)
pyhrf.verbose(2, 'bin threshold: %f' %bin_threshold)
pyhrf.verbose(2, 'pids(n=%d): %d...%d' \
%(len(p_ids),min(p_ids),max(p_ids)))
for i,p_id in enumerate(p_ids):
if p_id != 0:
new_p = np.zeros_like(d)
new_p[np.where(d==p_id)] = i + 1 #0 is background
ifn = op.join(tmp_dir,'pmask_%d.nii'%p_id)
write_volume(new_p, ifn, h)
data_files.append(ifn)
ofn = op.join(tmp_dir,'ptex_%d.gii'%p_id)
output_texs.append(ofn)
else:
data_files.append(fmri_data_file)
output_texs.append(output_tex)
else:
data_files.append(fmri_data_file)
output_texs.append(output_tex)
pyhrf.verbose(3, 'input data files: %s' %str(data_files))
pyhrf.verbose(3, 'output data files: %s' %str(output_texs))
for data_file, o_tex in zip(data_files, output_texs):
projection = [
'AimsFunctionProjection',
'-op', '1',
'-d', kernels_file,
'-d1', data_file,
'-m', input_mesh,
'-o', o_tex
]
cmd = ' '.join(map(str,projection))
pyhrf.verbose(3, 'cmd: %s' %cmd)
os.system(cmd)
if bin_threshold is not None:
pyhrf.verbose(2, 'Binary threshold of texture at %f' %bin_threshold)
o_tex = output_texs[0]
data,data_gii = read_texture(o_tex)
data = (data>bin_threshold).astype(np.int32)
print 'data:', data.dtype
if p_ids is not None:
for pid, o_tex in zip(p_ids[1:], output_texs[1:]):
pdata,pdata_gii = read_texture(o_tex)
data += (pdata>bin_threshold).astype(np.int32) * pid
#assert (np.unique(data) == p_ids).all()
write_texture(data, output_tex, intent='NIFTI_INTENT_LABEL')
def parcellation_for_jde(fmri_data, avg_parcel_size=250, output_dir=None,
method='gkm', glm_drift='Cosine', glm_hfcut=128):
"""
method: gkm, ward, ward_and_gkm
"""
if output_dir is None:
output_dir = tempfile.mkdtemp(prefix='pyhrf_JDE_parcellation_GLM',
dir=pyhrf.cfg['global']['tmp_path'])
glm_output_dir = op.join(output_dir, 'GLM_for_parcellation')
if not op.exists(glm_output_dir): os.makedirs(glm_output_dir)
pyhrf.verbose(1, 'GLM for parcellation')
# if fmri_data.data_type == 'volume':
# paradigm_file, bold_file, mask_file = fmri_data.save(glm_output_dir)
# beta_files = glm_nipy_from_files(bold_file, fmri_data.tr, paradigm_file,
# glm_output_dir, mask_file,
# drift_model=glm_drift, hfcut=glm_hfcut)
# elif fmri_data.data_type == 'surface':
# beta_files = glm_nipy(fmri_data, glm_output_dir,
# drift_model=glm_drift, hfcut=glm_hfcut)
g, dm, cons = glm_nipy(fmri_data, drift_model=glm_drift, hfcut=glm_hfcut)
pval_files = []
if cons is not None:
func_data = [('con_pval_%s' %cname, con.pvalue()) \
for cname, con in cons.iteritems()]
else:
reg_cst_drift = re.compile(".*constant.*|.*drift.*")
func_data = [('beta_%s' %reg_name, g.beta[ir]) \
for ir,reg_name in enumerate(dm.names) \
if not reg_cst_drift.match(reg_name)]
for name, data in func_data:
val_vol = expand_array_in_mask(data, fmri_data.roiMask>0)
val_fn = op.join(glm_output_dir, '%s.nii' %name)
write_volume(val_vol, val_fn, fmri_data.meta_obj)
pval_files.append(val_fn)
mask_file = op.join(glm_output_dir,'mask.nii')
write_volume(fmri_data.roiMask>0, mask_file, fmri_data.meta_obj)
nvox = fmri_data.get_nb_vox_in_mask()
nparcels = round_nb_parcels(nvox * 1. / avg_parcel_size)
pyhrf.verbose(1, 'Parcellation from GLM outputs, method: %s, ' \
'nb parcels: %d' %(method, nparcels))
if fmri_data.data_type == 'volume':
parcellation_file = op.join(output_dir, 'parcellation_%s_np%d.nii'
%(method, nparcels))
make_parcellation_from_files(pval_files, mask_file, parcellation_file,
nparcels, method)
parcellation,_ = read_volume(parcellation_file)
else:
mesh_file = fmri_data.data_files[-1]
parcellation_file = op.join(output_dir, 'parcellation_%s_np%d.gii'
%(method, nparcels))
make_parcellation_surf_from_files(pval_files, mesh_file,
parcellation_file, nparcels, method,
verbose=1)
parcellation,_ = read_texture(parcellation_file)
#print parcellation_file
pyhrf.verbose(1, parcellation_report(parcellation))
return parcellation, parcellation_file
def load_surf_bold_mask(bold_files, mesh_file, mask_file=None):
pyhrf.verbose(1, 'Load mesh: ' + mesh_file)
coords,triangles,coord_sys = read_mesh(mesh_file)
pyhrf.verbose(2, 'Build graph ... ')
fgraph = graph_from_mesh(triangles)
assert graph_is_sane(fgraph)
pyhrf.verbose(1, 'Mesh has %d nodes' %len(fgraph))
pyhrf.verbose(2, 'Compute length of edges ... ')
edges_l = np.array([np.array([distance(coords[i],
coords[n],
coord_sys.xform) \
for n in nl]) \
for i,nl in enumerate(fgraph)],dtype=object)
if mask_file is None or not op.exists(mask_file):
pyhrf.verbose(1,'Mask file %s does not exist. Taking '\
' all nodes ...' %mask_file)
mask = np.ones(len(fgraph))
mask_meta_obj = None
mask_loaded_from_file = False
else:
mask, mask_meta_obj = read_texture(mask_file)
mask_loaded_from_file = True
if not (np.round(mask) == mask).all():
raise Exception("Mask is not n-ary")
if len(mask) != len(fgraph):
raise Exception('Size of mask (%d) is different from size '\
'of graph (%d)' %(len(mask),len(fgraph)))
mask = mask.astype(np.int32)
if mask.min() == -1:
mask += 1
#Split graph into rois:
graphs = {}
edge_lengths = {}
for roiId in np.unique(mask):
mroi = np.where(mask==roiId)
g, nm = sub_graph(fgraph, mroi[0])
edge_lengths[roiId] = edges_l[mroi]
graphs[roiId] = g
#Load BOLD:
lastScan = 0
sessionScans = []
bolds = []
for boldFile in bold_files:
pyhrf.verbose(1, 'load bold: ' + boldFile)
b,_ = read_texture(boldFile)
pyhrf.verbose(1, 'bold shape: ' + str(b.shape))
bolds.append(b)
sessionScans.append(np.arange(lastScan, lastScan+b.shape[0],
dtype=int))
lastScan += b.shape[0]
bold = np.concatenate(tuple(bolds))
if len(fgraph) != bold.shape[1]:
raise Exception('Nb positions not consistent between BOLD (%d) '\
'and mesh (%d)' %(bold.shape[1],len(fgraph)))
# discard bad data (bold with var=0 and nan values):
discard_bad_data(bold, mask, time_axis=0)
return mask, mask_meta_obj, mask_loaded_from_file, bold, sessionScans, \
graphs, edge_lengths
def make_parcellation_surf_from_files(beta_files, mesh_file, parcellation_file,
nbparcel, method, mu=10., verbose=0):
if method not in ['ward', 'gkm', 'ward_and_gkm', 'kmeans']:
raise ValueError('unknown method')
# step 1: load the data ----------------------------
# 1.1 the domain
pyhrf.verbose(3, 'domain from mesh: %s' %mesh_file)
domain = domain_from_mesh(mesh_file)
coord = domain.coord
# 1.3 read the functional data
beta = np.array([read_texture(b)[0] for b in beta_files]).T
pyhrf.verbose(3, 'beta: %s' %str(beta.shape))
pyhrf.verbose(3, 'mu * coord / np.std(coord): %s' \
%(mu * coord / np.std(coord)).shape)
feature = np.hstack((beta, mu * coord / np.std(coord)))
if method is not 'kmeans':
# print 'domain.topology:', domain.topology.__class__
# print domain.topology
#print dir(domain.topology)
# print 'feature:', feature.shape
# print feature
g = field_from_coo_matrix_and_data(domain.topology, feature)
# print 'g:', g.__class__
# print g
if method == 'kmeans':
_, u, _ = kmeans(feature, nbparcel)
if method == 'ward':
u, _ = g.ward(nbparcel)
if method == 'gkm':
seeds = np.argsort(np.random.rand(g.V))[:nbparcel]
_, u, _ = g.geodesic_kmeans(seeds)
if method == 'ward_and_gkm':
w, _ = g.ward(nbparcel)
_, u, _ = g.geodesic_kmeans(label=w)
# print 'u:'
# print u
lpa = SubDomains(domain, u, 'parcellation')
if verbose:
var_beta = np.array(
[np.var(beta[lpa.label == k], 0).sum() for k in range(lpa.k)])
var_coord = np.array(
[np.var(coord[lpa.label == k], 0).sum() for k in range(lpa.k)])
size = lpa.get_size()
vf = np.dot(var_beta, size) / size.sum()
va = np.dot(var_coord, size) / size.sum()
print nbparcel, "functional variance", vf, "anatomical variance", va
# step3: write the resulting label image
if parcellation_file is not None:
label_image = parcellation_file
# elif write_dir is not None:
# label_image = os.path.join(write_dir, "parcel_%s.nii" % method)
else:
label_image = None
if label_image is not None:
#lpa.to_image(label_image, descrip='Intra-subject parcellation image')
write_texture(u.astype(np.int32), label_image)
if verbose:
print "Wrote the parcellation images as %s" % label_image
return u, label_image
