def test_load_with_archive_filepath_modified(self):
# Save an artifact for use in the following test case.
fp = os.path.join(self.test_dir.name, 'artifact.qza')
Artifact.import_data(FourInts, [-1, 42, 0, 43]).save(fp)
# Load the artifact from a filepath then save a different artifact to
# the same filepath. Assert that both artifacts produce the correct
# views of their data.
#
# `load` used to be lazy, only extracting data when it needed to (e.g.
# when `save` or `view` was called). This was buggy as the filepath
# could have been deleted, or worse, modified to contain a different
# .qza file. Thus, the wrong archive could be extracted on demand, or
# the archive could be missing altogether. There isn't an easy
# cross-platform compatible way to solve this problem, so Artifact.load
# is no longer lazy and always extracts its data immediately. The real
# motivation for lazy loading was for quick inspection of archives
# without extracting/copying data, so that API is now provided through
# Artifact.peek.
artifact1 = Artifact.load(fp)
Artifact.import_data(FourInts, [10, 11, 12, 13]).save(fp)
artifact2 = Artifact.load(fp)
self.assertEqual(artifact1.view(list), [-1, 42, 0, 43])
self.assertEqual(artifact2.view(list), [10, 11, 12, 13])
def test_load_with_archive_filepath_modified(self):
# Save an artifact for use in the following test case.
fp = os.path.join(self.test_dir.name, 'artifact.qza')
Artifact.import_data(FourInts, [-1, 42, 0, 43]).save(fp)
# Load the artifact from a filepath then save a different artifact to
# the same filepath. Assert that both artifacts produce the correct
# views of their data.
#
# `load` used to be lazy, only extracting data when it needed to (e.g.
# when `save` or `view` was called). This was buggy as the filepath
# could have been deleted, or worse, modified to contain a different
# .qza file. Thus, the wrong archive could be extracted on demand, or
# the archive could be missing altogether. There isn't an easy
# cross-platform compatible way to solve this problem, so Artifact.load
# is no longer lazy and always extracts its data immediately. The real
# motivation for lazy loading was for quick inspection of archives
# without extracting/copying data, so that API is now provided through
# Artifact.peek.
artifact1 = Artifact.load(fp)
Artifact.import_data(FourInts, [10, 11, 12, 13]).save(fp)
artifact2 = Artifact.load(fp)
self.assertEqual(artifact1.view(list), [-1, 42, 0, 43])
self.assertEqual(artifact2.view(list), [10, 11, 12, 13])
def test_filter_features_nooverlap(self):
ar_tree = Artifact.load(self.get_data_path('tree_reject.qza'))
ar_table = Artifact.load(
self.get_data_path('counts_nooverlap.biom.qza'))
with self.assertRaises(ValueError):
tbl_positive, tbl_negative = filter_features(
table=ar_table.view(biom.Table),
tree=ar_tree.view(NewickFormat))
def test_load_different_type_with_multiple_view_types(self):
saved_artifact = Artifact.import_data(IntSequence1,
[42, 42, 43, -999, 42])
fp = os.path.join(self.test_dir.name, 'artifact.qza')
saved_artifact.save(fp)
artifact = Artifact.load(fp)
self.assertEqual(artifact.type, IntSequence1)
self.assertEqual(artifact.uuid, saved_artifact.uuid)
self.assertEqual(artifact.view(list), [42, 42, 43, -999, 42])
self.assertEqual(artifact.view(list), [42, 42, 43, -999, 42])
self.assertEqual(artifact.view(collections.Counter),
collections.Counter({
42: 3,
43: 1,
-999: 1
}))
self.assertEqual(artifact.view(collections.Counter),
collections.Counter({
42: 3,
43: 1,
-999: 1
}))
def test_load_and_save(self):
fp1 = os.path.join(self.test_dir.name, 'artifact1.qza')
fp2 = os.path.join(self.test_dir.name, 'artifact2.qza')
artifact = Artifact.import_data(FourInts, [-1, 42, 0, 43])
artifact.save(fp1)
artifact = Artifact.load(fp1)
# Overwriting its source file works.
artifact.save(fp1)
# Saving to a new file works.
artifact.save(fp2)
root_dir = str(artifact.uuid)
expected = {
'VERSION', 'metadata.yaml', 'data/file1.txt', 'data/file2.txt',
'data/nested/file3.txt', 'data/nested/file4.txt',
'provenance/metadata.yaml', 'provenance/VERSION',
'provenance/action/action.yaml'
}
self.assertArchiveMembers(fp1, root_dir, expected)
root_dir = str(artifact.uuid)
expected = {
'VERSION', 'metadata.yaml', 'data/file1.txt', 'data/file2.txt',
'data/nested/file3.txt', 'data/nested/file4.txt',
'provenance/metadata.yaml', 'provenance/VERSION',
'provenance/action/action.yaml'
}
self.assertArchiveMembers(fp2, root_dir, expected)
def test_classify_otus_experimental(self):
ar_tree = Artifact.load(self.get_data_path('sepp_tree_tiny.qza'))
ar_repseq = Artifact.load(self.get_data_path('real_data.qza'))
obs_classification = classify_otus_experimental(
ar_repseq.view(DNASequencesDirectoryFormat),
ar_tree.view(NewickFormat))
exp_classification = pd.read_csv(
self.get_data_path('taxonomy_real_data_tiny_otus.tsv'),
index_col=0,
sep="\t").fillna("")
assert_frame_equal(obs_classification, exp_classification)
ar_tree_small = Artifact.load(
self.get_data_path('sepp_tree_small.qza'))
obs_classification_small = classify_otus_experimental(
ar_repseq.view(DNASequencesDirectoryFormat),
ar_tree_small.view(NewickFormat))
exp_classification_small = pd.read_csv(
self.get_data_path('taxonomy_real_data_small_otus.tsv'),
index_col=0,
sep="\t").fillna("")
assert_frame_equal(obs_classification_small, exp_classification_small)
ar_refphylo_tiny = Artifact.load(
self.get_data_path('reference_phylogeny_tiny.qza'))
ref_phylo_tiny = ar_refphylo_tiny.view(NewickFormat)
with self.assertRaises(ValueError):
classify_otus_experimental(
ar_repseq.view(DNASequencesDirectoryFormat), ref_phylo_tiny)
# test that missing taxon mappings result in an error
ar_taxonomy = Artifact.load(
self.get_data_path('taxonomy_missingotus.qza'))
# capture stderr message and check if its content is as expected
captured_stderr = StringIO()
with redirect_stderr(captured_stderr):
with self.assertRaises(ValueError):
classify_otus_experimental(
ar_repseq.view(DNASequencesDirectoryFormat),
ar_tree.view(NewickFormat),
reference_taxonomy=ar_taxonomy.view(pd.DataFrame))
self.assertIn('The taxonomy artifact you provided does not cont',
captured_stderr.getvalue())
self.assertIn('539572', captured_stderr.getvalue())
def test_eq_same_uuid(self):
fp = os.path.join(self.test_dir.name, 'artifact.qza')
artifact1 = Artifact.import_data(FourInts, [-1, 42, 0, 43])
artifact1.save(fp)
artifact2 = Artifact.load(fp)
self.assertEqual(artifact1, artifact2)
def test_eq_same_uuid(self):
fp = os.path.join(self.test_dir.name, 'artifact.qza')
artifact1 = Artifact.import_data(FourInts, [-1, 42, 0, 43])
artifact1.save(fp)
artifact2 = Artifact.load(fp)
self.assertEqual(artifact1, artifact2)
def test_roundtrip(self):
fp1 = os.path.join(self.test_dir.name, 'artifact1.qza')
fp2 = os.path.join(self.test_dir.name, 'artifact2.qza')
artifact = Artifact.import_data(FourInts, [-1, 42, 0, 43])
artifact.save(fp1)
artifact1 = Artifact.load(fp1)
artifact1.save(fp2)
artifact2 = Artifact.load(fp2)
self.assertEqual(artifact1.type, artifact2.type)
self.assertEqual(artifact1.format, artifact2.format)
self.assertEqual(artifact1.uuid, artifact2.uuid)
self.assertEqual(artifact1.view(list), artifact2.view(list))
# double view to make sure multiple views can be taken
self.assertEqual(artifact1.view(list), artifact2.view(list))
def test_roundtrip(self):
fp1 = os.path.join(self.test_dir.name, 'artifact1.qza')
fp2 = os.path.join(self.test_dir.name, 'artifact2.qza')
artifact = Artifact.import_data(FourInts, [-1, 42, 0, 43])
artifact.save(fp1)
artifact1 = Artifact.load(fp1)
artifact1.save(fp2)
artifact2 = Artifact.load(fp2)
self.assertEqual(artifact1.type, artifact2.type)
self.assertEqual(artifact1.format, artifact2.format)
self.assertEqual(artifact1.uuid, artifact2.uuid)
self.assertEqual(artifact1.view(list),
artifact2.view(list))
# double view to make sure multiple views can be taken
self.assertEqual(artifact1.view(list),
artifact2.view(list))
def test_load(self):
saved_artifact = Artifact.import_data(FourInts, [-1, 42, 0, 43])
fp = os.path.join(self.test_dir.name, 'artifact.qza')
saved_artifact.save(fp)
artifact = Artifact.load(fp)
self.assertEqual(artifact.type, FourInts)
self.assertEqual(artifact.uuid, saved_artifact.uuid)
self.assertEqual(artifact.view(list), [-1, 42, 0, 43])
self.assertEqual(artifact.view(list), [-1, 42, 0, 43])
def test_load(self):
saved_artifact = Artifact.import_data(FourInts, [-1, 42, 0, 43])
fp = os.path.join(self.test_dir.name, 'artifact.qza')
saved_artifact.save(fp)
artifact = Artifact.load(fp)
self.assertEqual(artifact.type, FourInts)
self.assertEqual(artifact.uuid, saved_artifact.uuid)
self.assertEqual(artifact.view(list), [-1, 42, 0, 43])
self.assertEqual(artifact.view(list), [-1, 42, 0, 43])
def test_exercise_sepp(self):
ar = Artifact.load(self.get_data_path('real_data.qza'))
view = ar.view(DNASequencesDirectoryFormat)
ar_refphylo = Artifact.load(
self.get_data_path('reference_phylogeny_small.qza'))
ref_phylo_small = ar_refphylo.view(NewickFormat)
ar_refaln = Artifact.load(
self.get_data_path('reference_alignment_small.qza'))
ref_aln_small = ar_refaln.view(AlignedDNASequencesDirectoryFormat)
obs_tree, obs_placements = sepp(view,
reference_alignment=ref_aln_small,
reference_phylogeny=ref_phylo_small)
tree = skbio.TreeNode.read(str(obs_tree))
obs = {n.name for n in tree.tips()}
seqs = {r.metadata['id'] for r in ar.view(DNAIterator)}
for seq in seqs:
self.assertIn(seq, obs)
def test_q_score_numeric_ids(self):
ar = Artifact.load(self.get_data_path('numeric_ids.qza'))
view = ar.view(SingleLanePerSampleSingleEndFastqDirFmt)
exp_sids = {'00123', '0.4560'}
obs, stats = q_score(view, min_quality=20)
obs_manifest = obs.manifest.view(obs.manifest.format)
obs_manifest = pd.read_csv(obs_manifest.open(), dtype=str, comment='#')
obs_manifest.set_index('sample-id', inplace=True)
obs_sids = set(obs_manifest.index)
self.assertEqual(obs_sids, exp_sids)
self.assertEqual(set(stats.index), exp_sids)
def test_ne_subclass_same_uuid(self):
class ArtifactSubclass(Artifact):
pass
fp = os.path.join(self.test_dir.name, 'artifact.qza')
artifact1 = ArtifactSubclass.import_data(FourInts, [-1, 42, 0, 43])
artifact1.save(fp)
artifact2 = Artifact.load(fp)
self.assertNotEqual(artifact1, artifact2)
self.assertNotEqual(artifact2, artifact1)
def test_ne_subclass_same_uuid(self):
class ArtifactSubclass(Artifact):
pass
fp = os.path.join(self.test_dir.name, 'artifact.qza')
artifact1 = ArtifactSubclass.import_data(FourInts, [-1, 42, 0, 43])
artifact1.save(fp)
artifact2 = Artifact.load(fp)
self.assertNotEqual(artifact1, artifact2)
self.assertNotEqual(artifact2, artifact1)
def test_q_score(self):
ar = Artifact.load(self.get_data_path('simple.qza'))
with redirected_stdio(stdout=os.devnull):
obs_drop_ambig_ar, stats_ar = self.plugin.methods['q_score'](
ar, quality_window=2, min_quality=20, min_length_fraction=0.25)
obs_drop_ambig = obs_drop_ambig_ar.view(
SingleLanePerSampleSingleEndFastqDirFmt)
stats = stats_ar.view(pd.DataFrame)
exp_drop_ambig = ["@foo_1",
"ATGCATGC",
"+",
"DDDDBBDD"]
columns = ['sample-id', 'total-input-reads', 'total-retained-reads',
'reads-truncated',
'reads-too-short-after-truncation',
'reads-exceeding-maximum-ambiguous-bases']
exp_drop_ambig_stats = pd.DataFrame([('foo', 2., 1., 0., 0., 1.),
('bar', 1., 0., 0., 0., 1.)],
columns=columns)
exp_drop_ambig_stats = exp_drop_ambig_stats.set_index('sample-id')
obs = []
iterator = obs_drop_ambig.sequences.iter_views(FastqGzFormat)
for sample_id, fp in iterator:
obs.extend([x.strip() for x in gzip.open(str(fp), 'rt')])
self.assertEqual(obs, exp_drop_ambig)
pdt.assert_frame_equal(stats, exp_drop_ambig_stats.loc[stats.index])
with redirected_stdio(stdout=os.devnull):
obs_trunc_ar, stats_ar = self.plugin.methods['q_score'](
ar, quality_window=1, min_quality=33, min_length_fraction=0.25)
obs_trunc = obs_trunc_ar.view(SingleLanePerSampleSingleEndFastqDirFmt)
stats = stats_ar.view(pd.DataFrame)
exp_trunc = ["@foo_1",
"ATGCATGC",
"+",
"DDDDBBDD",
"@bar_1",
"ATA",
"+",
"DDD"]
exp_trunc_stats = pd.DataFrame([('foo', 2., 1., 0., 0., 1.),
('bar', 1., 1., 1., 0., 0.)],
columns=columns)
exp_trunc_stats = exp_trunc_stats.set_index('sample-id')
obs = []
for sample_id, fp in obs_trunc.sequences.iter_views(FastqGzFormat):
obs.extend([x.strip() for x in gzip.open(str(fp), 'rt')])
self.assertEqual(sorted(obs), sorted(exp_trunc))
pdt.assert_frame_equal(stats, exp_trunc_stats.loc[stats.index])
def test_refmismatch(self):
ar_refphylo = Artifact.load(self.get_data_path(
'reference_phylogeny_small.qza'))
ref_phylo_small = ar_refphylo.view(NewickFormat)
ar_refaln = Artifact.load(self.get_data_path(
'reference_alignment_small.qza'))
ref_aln_small = ar_refaln.view(AlignedDNASequencesDirectoryFormat)
with self.assertRaises(ValueError):
sepp(None, reference_phylogeny=ref_phylo_small)
with self.assertRaises(ValueError):
sepp(None, reference_alignment=ref_aln_small)
ar_refphylo_tiny = Artifact.load(self.get_data_path(
'reference_phylogeny_tiny.qza'))
ref_phylo_tiny = ar_refphylo_tiny.view(NewickFormat)
with self.assertRaises(ValueError):
sepp(None, reference_alignment=ref_aln_small,
reference_phylogeny=ref_phylo_tiny)
def test_filter_features(self):
ar_tree = Artifact.load(self.get_data_path('tree_reject.qza'))
ar_table = Artifact.load(self.get_data_path('counts_reject.biom.qza'))
tbl_positive, tbl_negative = filter_features(
table=ar_table.view(biom.Table),
tree=ar_tree.view(NewickFormat)
)
self.assertEqual(tbl_positive.sum(), 715)
self.assertEqual(tbl_negative.sum(), 133)
exp_sample_ids = set(['sample_a', 'sample_b', 'sample_c', 'sample_d'])
self.assertEqual(set(tbl_positive.ids()) ^ exp_sample_ids, set())
self.assertEqual(set(tbl_negative.ids()) ^ exp_sample_ids, set())
exp_pos_feature_ids = set([
'testseqa', 'testseqb', 'testseqc', 'testseqd', 'testseqe',
'testseqf', 'testseqg', 'testseqh', 'testseqi', 'testseqj'])
self.assertEqual(set(tbl_positive.ids(
axis='observation')) ^ exp_pos_feature_ids, set())
exp_neg_feature_ids = set(['testseq_reject_1', 'testseq_reject_2'])
self.assertEqual(set(tbl_negative.ids(
axis='observation')) ^ exp_neg_feature_ids, set())
def test_q_score_numeric_ids(self):
ar = Artifact.load(self.get_data_path('numeric_ids.qza'))
exp_sids = {'00123', '0.4560'}
with redirected_stdio(stdout=os.devnull):
obs_ar, stats_ar = self.plugin.methods['q_score'](
ar, min_quality=20)
obs = obs_ar.view(SingleLanePerSampleSingleEndFastqDirFmt)
stats = stats_ar.view(pd.DataFrame)
obs_manifest = obs.manifest.view(obs.manifest.format)
obs_manifest = pd.read_csv(obs_manifest.open(), dtype=str, comment='#')
obs_manifest.set_index('sample-id', inplace=True)
obs_sids = set(obs_manifest.index)
self.assertEqual(obs_sids, exp_sids)
self.assertEqual(set(stats.index), exp_sids)
def test_load_and_save(self):
fp1 = os.path.join(self.test_dir.name, 'artifact1.qza')
fp2 = os.path.join(self.test_dir.name, 'artifact2.qza')
artifact = Artifact.import_data(FourInts, [-1, 42, 0, 43])
artifact.save(fp1)
artifact = Artifact.load(fp1)
# Overwriting its source file works.
artifact.save(fp1)
# Saving to a new file works.
artifact.save(fp2)
root_dir = str(artifact.uuid)
expected = {
'VERSION',
'checksums.md5',
'metadata.yaml',
'data/file1.txt',
'data/file2.txt',
'data/nested/file3.txt',
'data/nested/file4.txt',
'provenance/metadata.yaml',
'provenance/VERSION',
'provenance/citations.bib',
'provenance/action/action.yaml'
}
self.assertArchiveMembers(fp1, root_dir, expected)
root_dir = str(artifact.uuid)
expected = {
'VERSION',
'checksums.md5',
'metadata.yaml',
'data/file1.txt',
'data/file2.txt',
'data/nested/file3.txt',
'data/nested/file4.txt',
'provenance/metadata.yaml',
'provenance/VERSION',
'provenance/citations.bib',
'provenance/action/action.yaml'
}
self.assertArchiveMembers(fp2, root_dir, expected)
def test_q_score(self):
ar = Artifact.load(self.get_data_path('simple.qza'))
view = ar.view(SingleLanePerSampleSingleEndFastqDirFmt)
obs_drop_ambig, stats = q_score(view, quality_window=2,
min_length_fraction=0.25)
exp_drop_ambig = ["@foo_1",
"ATGCATGC",
"+",
"DDDDBBDD"]
columns = ['sample-id', 'total-input-reads', 'total-retained-reads',
'reads-truncated',
'reads-too-short-after-truncation',
'reads-exceeding-maximum-ambiguous-bases']
exp_drop_ambig_stats = pd.DataFrame([('foo', 2, 1, 0, 0, 1),
('bar', 1, 0, 0, 0, 1)],
columns=columns)
exp_drop_ambig_stats = exp_drop_ambig_stats.set_index('sample-id')
obs = []
iterator = obs_drop_ambig.sequences.iter_views(FastqGzFormat)
for sample_id, fp in iterator:
obs.extend([l.strip() for l in gzip.open(str(fp), 'rt')])
self.assertEqual(obs, exp_drop_ambig)
pdt.assert_frame_equal(stats, exp_drop_ambig_stats.loc[stats.index])
obs_trunc, stats = q_score(view, quality_window=1, min_quality=33,
min_length_fraction=0.25)
exp_trunc = ["@foo_1",
"ATGCATGC",
"+",
"DDDDBBDD",
"@bar_1",
"ATA",
"+",
"DDD"]
exp_trunc_stats = pd.DataFrame([('foo', 2, 1, 0, 0, 1),
('bar', 1, 1, 1, 0, 0)],
columns=columns)
exp_trunc_stats = exp_trunc_stats.set_index('sample-id')
obs = []
for sample_id, fp in obs_trunc.sequences.iter_views(FastqGzFormat):
obs.extend([l.strip() for l in gzip.open(str(fp), 'rt')])
self.assertEqual(sorted(obs), sorted(exp_trunc))
pdt.assert_frame_equal(stats, exp_trunc_stats.loc[stats.index])
def test_load_different_type_with_multiple_view_types(self):
saved_artifact = Artifact.import_data(IntSequence1,
[42, 42, 43, -999, 42])
fp = os.path.join(self.test_dir.name, 'artifact.qza')
saved_artifact.save(fp)
artifact = Artifact.load(fp)
self.assertEqual(artifact.type, IntSequence1)
self.assertEqual(artifact.uuid, saved_artifact.uuid)
self.assertEqual(artifact.view(list),
[42, 42, 43, -999, 42])
self.assertEqual(artifact.view(list),
[42, 42, 43, -999, 42])
self.assertEqual(artifact.view(collections.Counter),
collections.Counter({42: 3, 43: 1, -999: 1}))
self.assertEqual(artifact.view(collections.Counter),
collections.Counter({42: 3, 43: 1, -999: 1}))
def export_artifact(uuid):
output = request.get_json().get('path')
Artifact.load(ARTIFACTS[uuid]).export_data(output)
return jsonify({'path': output})
def classify_otus_experimental(
representative_sequences: DNASequencesDirectoryFormat,
tree: NewickFormat,
reference_taxonomy: pd.DataFrame = None) -> pd.DataFrame:
if reference_taxonomy is None:
filename_default_taxonomy = os.path.join(_sepp_refs_path(),
'taxonomy_gg99.qza')
reference_taxonomy = Artifact.load(filename_default_taxonomy).view(
pd.DataFrame)
# convert type of feature IDs to str (depending on pandas type inference
# they might come as integers), to make sure they are of the same type as
# in the tree.
reference_taxonomy.index = map(str, reference_taxonomy.index)
# load the insertion tree
tree = skbio.TreeNode.read(str(tree))
# ensure that all reference tips in the tree (those without the inserted
# fragments) have a mapping in the user provided taxonomy table
names_tips = {node.name for node in tree.tips()}
names_fragments = {
fragment.metadata['id']
for fragment in representative_sequences.file.view(DNAIterator)
}
missing_features = (names_tips - names_fragments) -\
set(reference_taxonomy.index)
if len(missing_features) > 0:
# QIIME2 users can run with --verbose and see stderr and stdout.
# Thus, we here report more details about the mismatch:
sys.stderr.write(
("The taxonomy artifact you provided does not contain lineage "
"information for the following %i features:\n%s") %
(len(missing_features), "\n".join(missing_features)))
raise ValueError("Not all OTUs in the provided insertion tree have "
"mappings in the provided reference taxonomy.")
taxonomy = []
for fragment in representative_sequences.file.view(DNAIterator):
# for every inserted fragment we now try to find the closest OTU tip
# in the tree and available mapping from the OTU-ID to a lineage
# string:
lineage_str = np.nan
# first, let us check if the fragment has been inserted at all ...
try:
curr_node = tree.find(fragment.metadata['id'])
except skbio.tree.MissingNodeError:
continue
# if yes, we start from the inserted node and traverse the tree as less
# as possible towards the root and check at every level if one or
# several OTU-tips are within the sub-tree.
if curr_node is not None:
foundOTUs = []
# Traversal is stopped at a certain level, if one or more OTU-tips
# have been found in the sub-tree OR ... (see break below)
while len(foundOTUs) == 0:
# SEPP insertion - especially for multiple very similar
# sequences - can result in a rather complex topology change
# if all those sequences are inserted into the same branch
# leading to one OTU-tip. Thus, we cannot simply visit only
# all siblings or decendents and rather need to traverse the
# whole sub-tree. Average case should be well behaved,
# thus I think it is ok.
for node in curr_node.postorder():
if (node.name is not None) and \
(node.name in reference_taxonomy.index):
# if a suitable OTU-tip node is found AND this OTU-ID
# has a mapping in the user provided reference_taxonomy
# we store the OTU-ID in the growing result list
foundOTUs.append(node.name)
# ... if the whole tree has been traversed without success,
# e.g. if user provided reference_taxonomy did not contain any
# matching OTU-IDs.
if curr_node.is_root():
break
# prepare next while iteration, by changing to the parent node
curr_node = curr_node.parent
if len(foundOTUs) > 0:
# If the above method has identified exactly one OTU-tip,
# resulting lineage string would simple be the one provided by
# the user reference_taxonomy. However, if the inserted
# fragment cannot unambiguously places into the reference tree,
# the above method will find multiple OTU-IDs, which might have
# lineage strings in the user provided reference_taxonomy that
# are similar up to a certain rank and differ e.g. for genus
# and species.
# Thus, we here find the longest common prefix of all lineage
# strings. We don't operate per character, but per taxonomic
# rank. Therefore, we first "convert" every lineage sting into
# a list of taxa, one per rank.
split_lineages = []
for otu in foundOTUs:
# find lineage string for OTU
lineage = reference_taxonomy.loc[otu, 'Taxon']
# necessary to split lineage apart to ensure that
# the longest common prefix operates on atomic ranks
# instead of characters
split_lineages.append(
list(map(str.strip, lineage.split(';'))))
# find the longest common prefix rank-wise and concatenate to
# one lineage string, separated by ;
lineage_str = "; ".join(os.path.commonprefix(split_lineages))
taxonomy.append({
'Feature ID': fragment.metadata['id'],
'Taxon': lineage_str
})
pd_taxonomy = pd.DataFrame(taxonomy)
# test if dataframe is completely empty, or if no lineages could be found
if (len(taxonomy) == 0) or \
(pd_taxonomy['Taxon'].dropna().shape[0] == 0):
raise ValueError(
("None of the representative-sequences can be found in the "
"insertion tree. Please double check that both inputs match up, "
"i.e. are results from the same 'sepp' run."))
return pd_taxonomy.set_index('Feature ID')
def load_artifacts(**kwargs):
return {
k: Artifact.load(ARTIFACTS[v])
for k, v in kwargs.items() if v != ''
}
def test_q_score_all_dropped(self):
ar = Artifact.load(self.get_data_path('simple.qza'))
with self.assertRaisesRegex(ValueError, "filtered out"):
with redirected_stdio(stdout=os.devnull):
self.plugin.methods['q_score'](ar, min_quality=50)
def test_q_score_real_joined(self):
ar = Artifact.load(self.get_data_path('real_data_joined.qza'))
view = ar.view(SingleLanePerSampleSingleEndFastqDirFmt)
obs_result, stats = q_score_joined(
view, min_quality=40, min_length_fraction=0.24)
# All input reads are represented here in their post-quality filtered
# form. Reads that are commented out were manually identified as being
# filtered by the q_score method. For the commented reads, the comments
# denote why the read is not retained.
# The first read, @HWI-EAS440_0386:1:32:15467:1432#0/1, is 25% of
# total read length and is indicative of a sequence at the
# min_length_fraction boundary.
exp_result = [
"@HWI-EAS440_0386:1:32:15467:1432#0/1",
"TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTT",
"+",
"hhhhhhhhhhhhfghhhghghghhhchhhahhhhhfhh",
# too short
# "@HWI-EAS440_0386:1:36:9986:17043#0/1",
# "TACGTAGGTGGCAAGCGTTATCCGGATTTATTG",
# "+",
# "hhhhhhhhhhhhhhhhhhhhhhhhhffhhghhh",
"@HWI-EAS440_0386:1:37:13343:14820#0/1",
"TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGAT"
"GGATGTTTAAGTCAGTTGTG",
"+",
"hhhhhhhhhhhhhfhhhhhfhhhhghhhhghhhhhhhhhgghhhgghhhgghh"
"hgdhhhhghghhhdhhhhgh",
"@HWI-EAS440_0386:1:41:18215:15404#0/1",
"TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA",
"+",
"hhhhhhhhhhhhghhhhhhhhhhhhffhhghhhhghhghgghghhhhhgh",
# too short
# "@HWI-EAS440_0386:1:42:5423:19606#0/1",
# "TACGTAGGGAGCAAGCGTT",
# "+",
# "hhhhghhhhhhhhhghhfh",
"@HWI-EAS440_0386:1:52:7507:5841#0/1",
"TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTT",
"+",
"hhhhhhhhhghhfghhhhhhhhhhgfhhhghhhghdhh",
"@HWI-EAS440_0386:1:53:18599:4074#0/1",
"TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTG",
"+",
"hhhhfhhhhhfhhhhhhfhffhghhfgghggghdcbh",
# too short
# "@HWI-EAS440_0386:1:55:16425:9514#0/1",
# "TACGGAGGATCCGAGCGTTATCCGGATT",
# "+",
# "hhhhhhhhhhhhfghhhghghhhhhbgh",
"@HWI-EAS440_0386:1:65:12049:5619#0/1",
"TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTG",
"+",
"hhhhhhhhhhhhhhhhhhhhhhhhhfhhhhhhhghdhghhhhhghcfh",
# @HWI-EAS440_0386:1:95:4837:16388#0/1
# starts off < Q40
]
columns = ['sample-id', 'total-input-reads', 'total-retained-reads',
'reads-truncated',
'reads-too-short-after-truncation',
'reads-exceeding-maximum-ambiguous-bases']
exp_stats = pd.DataFrame([('foo', 10, 6, 10, 4, 0)],
columns=columns)
exp_stats = exp_stats.set_index('sample-id')
obs = []
iterator = obs_result.sequences.iter_views(FastqGzFormat)
for sample_id, fp in iterator:
obs.extend([l.strip() for l in gzip.open(str(fp), 'rt')])
self.assertEqual(obs, exp_result)
pdt.assert_frame_equal(stats, exp_stats.loc[stats.index])
def test_q_score_all_dropped(self):
ar = Artifact.load(self.get_data_path('simple.qza'))
view = ar.view(SingleLanePerSampleSingleEndFastqDirFmt)
with self.assertRaisesRegex(ValueError, "filtered out"):
q_score(view, min_quality=50)
def export_artifact(uuid):
output = request.get_json().get('path')
Artifact.load(ARTIFACTS[uuid]).export_data(output)
return jsonify({'path': output})
def load_artifacts(**kwargs):
return {k: Artifact.load(ARTIFACTS[v]) for k, v in kwargs.items()
if v != ''}
