def compare_synapses(filename, param_list=None, errors=0):
"""Run simulations with different number of (E,I) synapses"""
if type(param_list) is tuple:
skip, syn_list = param_list
else:
syn_list = param_list
skip = SKIP_IF_EXISTS
if not syn_list:
syn_list = [(120, 10), (140, 50), (180, 130)]
print("syn_list:{}".format(syn_list))
load_file(filename)
load_file(filename)
vm, cli = get_base_vm_cli(filename)
h.v_init = vm
for i, (exc, inh) in enumerate(syn_list):
h.newSynapses(exc, inh)
try:
save_run("{}_{},{}".format(filename, exc, inh), set_cli=cli, skip_if_exists=skip)
except RuntimeError as re:
print("Error {} occured on E={} & I={}".format(re, exc, inh))
print("leftover params = {}".format(syn_list[i:]))
if errors == 2:
print("second time error occured, bailing")
import sys
sys.exit(-1)
else:
compare_synapses(filename, param_list=syn_list[i:], errors=errors + 1)
def compare_pkcc2_homo(filename, param_list=None):
"""Change homogeneity of KCC2 pump strength"""
axon_pkcc2_list = [1]
ldend_pkcc2_list = [0.5, 1, 2, 4]
if param_list is not None:
if np.iterable(param_list[0]) and type(param_list[0]) is not str:
ldend_pkcc2_list, axon_pkcc2_list = param_list
else:
ldend_pkcc2_list = param_list
base = 1.9297e-5
print("ldend_pkcc2_list:{}".format(ldend_pkcc2_list))
load_file(filename)
if "KCC2" not in filename:
# run a single simulation without KCC2.
savename = "{}_({:.2f},{:.2f})".format(filename, 0, 0)
save_run(savename)
return
cmd_bdend = "bdend.Pa_KCC2 = {}".format(base)
h(cmd_bdend)
for pkcc2 in ldend_pkcc2_list:
cmd = "ldend.Pa_KCC2 = {}".format(base*pkcc2)
print(cmd)
h(cmd)
for axon_pkcc2 in axon_pkcc2_list:
axon_cmd = "axon.Pa_KCC2 = {}".format(base*axon_pkcc2)
print(axon_cmd)
h(axon_cmd)
save_run("{}_({:.2f},{:.2f})".format(filename, pkcc2, axon_pkcc2))
def compare_weights(filename, param_list=None):
"""Run Simulations with different strengths of synapses (keeping the actual number from load_config the same.
"""
if param_list:
# keep param_list a list, but make first (only) arg a dict
weights_dict = param_list[0]
assert weights_dict['inh_weights']
assert weights_dict['exc_weights']
else:
weights_dict = {
'exc_weights': [10],
'inh_weights': [10]
}
print("weight_dict:{}".format(weights_dict))
load_file(filename)
# load config
load_config()
# define hoc-accessible Vectors (lists)
h("objref inhibWeights_config,exciteWeights_config")
# convert python lists to NEURON Vectors
h.inhibWeights_config = h.Vector(weights_dict['inh_weights'])
h.exciteWeights_config = h.Vector(weights_dict['exc_weights'])
# assign weight vectors during configWeights (which is called from (if not persistent synapses)
# run.hoc > doRun() > loadParameters() > protocolStimVectors() > [proximal|distal]HzConfig > configWeights()
h("""
proc configWeights(){
print "configWeights custom Python"
inhibWeights = inhibWeights_config
exciteWeights = exciteWeights_config
}
""")
# iterating over weights is already done in run.hoc
save_run("{}".format(filename))
def compare_diam(filename, param_list=None):
"""Change distal dendrite's diameter"""
diam_list = param_list
if not diam_list:
diam_list = [0.5, 1, 1.5, 2]
print("diam_list:{}".format(diam_list))
load_file(filename)
for diam in diam_list:
h("""ldend.diam = {}""".format(diam))
save_run("{}_{}".format(filename, diam))
def compare_pCl(filename, param_list=None):
"""Change leak chloride permeability (ratio to leak potassium conductance)"""
pcl_list = param_list
if not pcl_list:
pcl_list = [0.1, 0.4, 0.8, 1.2]
print("pcl_list:{}".format(pcl_list))
load_file(filename)
# list of (exc,inh) synapses numbers
for pcl in pcl_list:
h.changePCl(pcl)
save_run("{}_{}".format(filename, pcl))
def compare_dynamic_K(filename, param_list=None):
"""Run simulations with dynamic potassium (True) or not (False)
The base filename should be used (e.g. 'distal') such that
- distal is the baseline
- distal_KCC2_True is KCC2 WITH dynamic potassium and
- distal_KCC2_False is KCC2 WITHOUT dynamic potassium
"""
if 'KCC2' in filename:
print("ignoring {} in 'compare_dynamic_K' because param_list should cover this case".format(filename))
return
K_list = param_list
if not K_list:
K_list = ['control', True, False]
print("K_list:{}".format(K_list))
for dyn_K in K_list:
fname = filename if dyn_K == 'control' else filename + "_KCC2"
assert (dyn_K != 'control' and 'KCC2' in fname) or (dyn_K == 'control' and 'KCC2' not in fname)
load_file(fname)
# get steady state here as it isn't done in run.hoc anymore with the set_cli arg
vm, cli = get_base_vm_cli(fname, compartment=h.ldend)
h.v_init = vm
if dyn_K == 'control':
print("control (no KCC2 nor KCC2_pot")
cmd = "forall{" + """
cli={cli}
cli0_cl_ion={cli}
""".format(cli=cli) +\
"}"
h(cmd)
save_run("{}_{}".format(fname, 0), set_cli=cli)
pot = ""
elif dyn_K:
# then add KCC2 potassium
cmd = """forall{
uninsert pasghk
uninsert KCC2
insert pas
insert KCC2_pot
g_pas = 0.00021
}"""
h(cmd)
pot = "_pot"
else:
# already have KCC2 in the neuron
pot = ""
cmd = "forall{" + """
cli={cli}
cli0_cl_ion={cli}
cli0_KCC2{pot}={cli}
""".format(cli=cli, pot=pot) +\
"}"
h(cmd)
save_run("{}_{}".format(fname, int(dyn_K)), set_cli=cli)
def compare_synapses_clumped(filename, param_list=None):
"""Run simulations with synapses located close together. Clumping implemented in `methods.hoc`
"""
syn_list = param_list
if not syn_list:
syn_list = [(120, 10), (140, 50), (180, 130)]
print("syn_list:{}".format(syn_list))
load_file(filename)
for (exc, inh, clump) in syn_list:
h.newSynapses(exc, inh)
h.clumpSynapses(clump)
save_run("{}_{},{}".format(filename, exc, inh))
def just_run(filename, param_list=None):
"""
Proxy for save_run, that accepts a param_list as expected in `nrnpy.start`
"""
load_file(filename)
if param_list is not None:
if type(param_list) is str:
h(param_list)
elif type(param_list[0]) is str:
for cmd in param_list:
h(cmd)
save_run(filename)
def compare_duration(filename, param_list=None):
"""Change tstop to compare"""
tstop_list = param_list
if not tstop_list:
base = h.tstop_config
tstop_list = [base/10, base/2, base, base*2, base*10]
print("tstop_list:{}".format(tstop_list))
load_file(filename)
for tstop in tstop_list:
h.tstop_config = tstop # doRun() calles loadParameters() in run.hoc which retrieves config variables
h.tstop = tstop # we set this here anyway
save_run("{}_{}".format(filename, tstop))
def compare_pkcc2(filename, param_list=None):
"""Change pump-strength of KCC2"""
pkcc2_list = param_list
if not pkcc2_list:
base = 1.9297e-5 # default value from mod file
pkcc2_list = [base/2, base, base*2, base*4]
print("pkcc2_list:{}".format(pkcc2_list))
load_file(filename)
for pkcc2 in pkcc2_list:
cmd = "forall{" +\
"Pa_KCC2 = {}".format(pkcc2) +\
"}"
h(cmd)
save_run("{}_{}".format(filename, pkcc2))
def compare_synapse_types(filename, param_list=None):
"""Run simulations with different synapse types. Note that the strength of these synapses will be wildy off.
* 0 - frequency-based ('f-in') synapses (receives input via NETSTIM)
* 1 - fluctuating conductance 'gclamp' synapses
* 2 - exc is 'f-in', inh is 'gclamp'
* 3 - inh is 'f-in', exc is 'gclamp'
"""
syn_type_list = param_list
if not syn_type_list:
syn_type_list = [0, 1, 2, 3]
print("syn_type_list:{}".format(syn_type_list))
load_file(filename)
for type_num in syn_type_list:
h.changeSynapseType(type_num)
save_run("{}_{}".format(filename, type_num))
def compare_cli(filename, param_list=None):
"""Change internal chloride concentration (for constant/static) runs"""
cli_list = param_list
if not cli_list:
cli_list = [2.5, 5, 7.5, 10, 12.5, 15, 20, 25, 30]
print("cli_list:{}".format(cli_list))
load_file(filename)
for cli in cli_list:
# chloride is set inside run.hoc through save_run --> doRun(set_cli)
cmd = "forall{" + """
cli={cli}
cli0_cl_ion={cli}
cli0_KCC2={cli}
""".format(cli=cli) +\
"}"
h(cmd)
save_run("{}_{}".format(filename, cli), set_cli=cli)
def compare_pas(filename, param_list=None):
"""Change input resistance by adjusting the passive leak channel.
The leak channel has K+, Na+, and Cl- conductances with a set ratio (see config.hoc).
Calling h.changePas(<new passive K conductance>) respects the ratio.
Saved filenames include the relative change in PAS, the Input Resistance, the steady-state cli (at 0 input),
and membrane potential.
"""
rel_gkpbar = param_list
if not rel_gkpbar:
rel_gkpbar = [0.8, 1, 1.2, 1.5]
print("rel_gkpbar:{}".format(rel_gkpbar))
load_file(filename)
# list of (exc,inh) synapses numbers
imp = h.Impedance()
imp.loc(0.5, sec=h.soma)
for p in rel_gkpbar:
h.changePas(h.g_pas_k_config*p)
vm, cli = get_base_vm_cli(filename, compartment=h.soma)
imp.compute(0, 1) # calculate impedance at 0 Hz (i.e. resistance)
ir = imp.input(0.5, sec=h.soma) # input resistance at 0.5
print("when p={}, Rm={}, [Cl]i={}, and Vm={}".format(p, ir, cli, vm))
save_run("{}_[{},{:.2f},{:.2f},{:.2f}]".format(filename, p, ir, cli, vm))
