def main_cosmic(options):
"""Main function used to process COSMIC data."""
# get configs
in_opts = _utils.get_input_config('classifier')
out_opts = _utils.get_output_config('features')
count_opts = _utils.get_output_config('feature_matrix')
# result_opts = _utils.get_input_config('result')
db_cfg = _utils.get_db_config('2020plus')
# get mutations
conn = sqlite3.connect(db_cfg['db'])
sql = ("SELECT Gene, Protein_Change as AminoAcid, "
" DNA_Change as Nucleotide, "
" Variant_Classification, "
" Tumor_Sample, Tumor_Type "
"FROM mutations")
mut_df = psql.frame_query(sql, con=conn)
conn.close()
# get features for classification
all_features = futils.generate_features(mut_df, options)
# save features to text file
cols = all_features.columns.tolist()
new_order = ['gene'] + cols[:cols.index('gene')] + cols[cols.index('gene')+1:]
all_features = all_features[new_order] # make the gene name the first column
out_path = _utils.save_dir + in_opts['gene_features'] if not options['output'] else options['output']
all_features.to_csv(out_path, sep='\t', index=False)
def main_cosmic(options):
"""Main function used to process COSMIC data."""
# get configs
in_opts = _utils.get_input_config('classifier')
out_opts = _utils.get_output_config('features')
count_opts = _utils.get_output_config('feature_matrix')
# result_opts = _utils.get_input_config('result')
db_cfg = _utils.get_db_config('2020plus')
# get mutations
conn = sqlite3.connect(db_cfg['db'])
sql = ("SELECT Gene, Protein_Change as AminoAcid, "
" DNA_Change as Nucleotide, "
" Variant_Classification, "
" Tumor_Sample, Tumor_Type "
"FROM mutations")
mut_df = psql.frame_query(sql, con=conn)
conn.close()
# get features for classification
all_features = futils.generate_features(mut_df, options)
# save features to text file
cols = all_features.columns.tolist()
new_order = ['gene'
] + cols[:cols.index('gene')] + cols[cols.index('gene') + 1:]
all_features = all_features[
new_order] # make the gene name the first column
out_path = _utils.save_dir + in_opts['gene_features'] if not options[
'output'] else options['output']
all_features.to_csv(out_path, sep='\t', index=False)
def main(cli_opts):
cfg_opts = _utils.get_output_config('classifier')
in_opts = _utils.get_input_config('classifier')
minimum_ct = cli_opts['min_count']
# get path to features used for classification
if cli_opts['features']:
feature_path = cli_opts['features']
else:
feature_path = _utils.save_dir + in_opts['gene_feature']
# read in features
df = pd.read_csv(feature_path,
sep='\t', index_col=0)
logger.info('Training R\'s Random forest . . .')
rrclf = RRandomForest(df,
other_sample_ratio=cli_opts['other_ratio'],
driver_sample=cli_opts['driver_rate'],
ntrees=cli_opts['ntrees'],
seed=cli_opts['random_seed'])
# train on entire data
if cli_opts['cv']:
rrclf.train_cv()
else:
rrclf.train()
logger.info('Finished training.')
logger.info('Saving classifier to . . .')
if cli_opts['cv']:
rrclf.clf.save_cv(cli_opts['output'])
else:
rrclf.clf.save(cli_opts['output'])
logger.info('Finished saving classifier.')
def main(cli_opts):
cfg_opts = _utils.get_output_config('classifier')
in_opts = _utils.get_input_config('classifier')
minimum_ct = cli_opts['min_count']
# get path to features used for classification
if cli_opts['features']:
feature_path = cli_opts['features']
else:
feature_path = _utils.save_dir + in_opts['gene_feature']
# read in features
df = pd.read_csv(feature_path, sep='\t', index_col=0)
logger.info('Training R\'s Random forest . . .')
rrclf = RRandomForest(df,
other_sample_ratio=cli_opts['other_ratio'],
driver_sample=cli_opts['driver_rate'],
ntrees=cli_opts['ntrees'],
seed=cli_opts['random_seed'])
# train on entire data
if cli_opts['cv']:
rrclf.train_cv()
else:
rrclf.train()
logger.info('Finished training.')
logger.info('Saving classifier to . . .')
if cli_opts['cv']:
rrclf.clf.save_cv(cli_opts['output'])
else:
rrclf.clf.save(cli_opts['output'])
logger.info('Finished saving classifier.')
def main(hypermutator_count, mut_path, db_path, no_cosmic_flag, opts):
"""Concatenates all the mutation data from tab delmited files in
the cosmic directory. Next, saves the results to a sqlite db.
Parameters
----------
hypermutator_count : int
remove samples with too many mutations
mut_path : str
Either path to directory containing contents of COSMIC's
genes.tgz file or decompressed CosmicMutantExport.tsv.
If empty string, just use path from config file.
db_path : str
path to save sqlite database. If string is empty,
use path from config.
no_cosmic_flag : bool
indicates not to use cosmic mutations
"""
# get input/output configurations
in_opts = _utils.get_input_config('input')
cosmic_path = in_opts['cosmic_path']
out_opts = _utils.get_output_config('gene_tsv')
out_path = out_opts['gene_tsv']
cnv_path = out_opts['cnv_tsv']
db_opts = _utils.get_db_config('2020plus')
out_db = db_opts['db']
# check if user specifies non standard db path
out_db = db_path if db_path else out_db
# save info into a txt file and sqlite3 database
if not no_cosmic_flag:
cosmic_path = mut_path if mut_path else cosmic_path
if os.path.isdir(cosmic_path):
# concatenate all gene files
concatenate_genes(out_path, cosmic_path)
# save database
save_db(hypermutator_count,
out_path,
out_db,
is_genes_tgz=True,
only_genome_wide=opts['only_genome_wide'],
use_unknown_status=opts['use_unknown_status'])
elif os.path.isfile(cosmic_path):
# save database
save_db(hypermutator_count,
cosmic_path,
out_db,
is_genes_tgz=False,
only_genome_wide=opts['only_genome_wide'],
use_unknown_status=opts['use_unknown_status'])
else:
raise ValueError('Please specify a vlid path to COSMIC data')
else:
# create an empty table if cosmic not wanted
create_empty_cosmic_mutation_table(out_db)
def sample_boxplot(pred_onco,
pred_tsg,
pred_driver,
save_path_type,
save_path_driver,
xlabel='',
ylabel='',
title=''):
"""Create a box plot for distribution of percentage of tumor samples
containing a non-silent mutation in different categories of genes (ie
oncogenes, tsgs, and drivers).
Parameters
----------
pred_onco : list
list of genes predicted as oncogenes
pred_tsg : list
list of genes predicted as tsgs
pred_driver : list
list of genes predicted as drivers
save_path_type : str
path to save figure for comparing oncogenes, tsgs, and other
save_path_driver : str
path to save figure for comparing drivers vs other
xlabel : str
x-axis label
ylabel : str
y-axis label
title : str
title of figures
"""
cfg = _utils.get_output_config('sample')
df = pd.read_csv(_utils.result_dir + cfg['max_gene_pct_sample_out'],
sep='\t',
index_col=0)
df['Predicted Type'] = [("oncogene" if g in pred_onco else "other")
for g in df.index]
df.ix[pred_tsg, 'Predicted Type'] = 'TSG'
df['Predicted Driver'] = [("driver" if g in pred_driver else "other")
for g in df.index]
# set figure labels
if not xlabel:
xlabel = 'Predicted Type'
if not ylabel:
ylabel = 'Maximum Pct of Samples for a Tumor Type'
if not title:
title = 'Percentage of Samples with Non-Silent Mutation'
# plot with oncogenes, tsgs, and other
myplt.boxplot(df,
by='Predicted Type',
column=['all mutation sample pct', 'non-silent sample pct'],
save_path=save_path_type,
xlabel=xlabel,
ylabel=ylabel,
title=title)
# plot with drivers vs other
myplt.boxplot(df,
by='Predicted Driver',
column=['all mutation sample pct', 'non-silent sample pct'],
save_path=save_path_driver,
xlabel=xlabel,
ylabel=ylabel,
title=title)
def retrieve_gene_features(conn, opts, get_entropy=True):
"""Retrieve gene information from the gene_features table.
See the gene_features module to understand the gene_features
database table.
Parameters
----------
conn : mysql/sqlite connection
connection to db with gene_features table
options : dict
options for getting info
get_entropy : bool
option to togle the use of entropy features.
Since entropy features are read from a file in this function, it may
induce a not necessary dependency on previously running commands.
To avoid this, set get_entropy=False and then compute entropy features
separately.
Returns
-------
df : pd.dataframe
dataframe of gene lengths
"""
logger.info('Retrieving features of genes . . .')
selected_cols = ['gene']
# retrieve more features if specified by command line
if opts['gene_length']:
selected_cols.append('gene_length')
if opts['mutation_rate']:
selected_cols.append('noncoding_mutation_rate')
if opts['replication_time']:
selected_cols.append('replication_time')
if opts['expression']:
selected_cols.append('expression_CCLE as expression')
if opts['hic']:
selected_cols.append('HiC_compartment')
if opts['betweeness']:
selected_cols.append('gene_betweeness')
if opts['degree']:
selected_cols.append('gene_degree')
# get info from gene_features table
logger.info(
'Retrieving gene feature information from gene_features table . . . ')
sql = "SELECT %s FROM gene_features" % ', '.join(selected_cols)
df = psql.frame_query(sql, conn)
df = df.set_index('gene')
df['gene'] = df.index
logger.info('Finished retrieving gene features from gene_features table.')
# fill graph stats with zeros if gene not in Biogrid
if 'gene_betweeness' in df.columns:
df['gene_betweeness'] = df['gene_betweeness'].fillna(0)
if 'gene_degree' in df.columns:
df['gene_degree'] = df['gene_degree'].fillna(0)
# get position entropy features
if get_entropy:
entropy_cfg = _utils.get_output_config('position_entropy')
mutation_pos_entropy = pd.read_csv(_utils.result_dir +
entropy_cfg['mutation_pos_entropy'],
sep='\t',
index_col=0)
missense_pos_entropy = pd.read_csv(_utils.result_dir +
entropy_cfg['missense_pos_entropy'],
sep='\t',
index_col=0)
#df['mutation position entropy'] = mutation_pos_entropy['mutation position entropy']
#df['pct of uniform mutation entropy'] = mutation_pos_entropy['pct of uniform mutation entropy']
df['missense position entropy'] = missense_pos_entropy[
'missense position entropy']
df['pct of uniform missense entropy'] = missense_pos_entropy[
'pct of uniform missense entropy']
return df
def main(cli_opts):
cfg_opts = _utils.get_output_config('classifier')
in_opts = _utils.get_input_config('classifier')
minimum_ct = cli_opts['min_count']
# get path to features used for classification
if cli_opts['features']:
feature_path = cli_opts['features']
else:
feature_path = _utils.save_dir + in_opts['gene_feature']
# read in null distribution p-values
if not cli_opts['simulated'] and cli_opts['null_distribution']:
null_pvals = pd.read_csv(cli_opts['null_distribution'], sep='\t',
index_col=0)
else:
null_pvals = None
# use trained classifier if provided
if cli_opts['trained_classifier']:
# read in features
df = pd.read_csv(feature_path, sep='\t', index_col=0)
logger.info('Running Random forest . . .')
# initialize R's random forest
rrclf = RRandomForest(df,
other_sample_ratio=cli_opts['other_ratio'],
driver_sample=cli_opts['driver_rate'],
ntrees=cli_opts['ntrees'],
seed=cli_opts['random_seed'])
# load classifier depending on whether it uses CV
is_cv = cli_opts['cv']
if is_cv:
rrclf.clf.load_cv(cli_opts['trained_classifier'])
else:
rrclf.clf.load(cli_opts['trained_classifier'])
if cli_opts['simulated']:
# do classification
result_df = trained_rand_forest_pred(rrclf, df, None, null_pvals, is_cv)
# driver scores
driver_score_cts = result_df['driver score'].value_counts()
driver_score_cts = driver_score_cts.sort_index(ascending=False)
driver_score_cum_cts = driver_score_cts.cumsum()
driver_score_pvals = driver_score_cum_cts / float(driver_score_cts.sum())
# oncogene scores
onco_score_cts = result_df['oncogene score'].value_counts()
onco_score_cts = onco_score_cts.sort_index(ascending=False)
onco_score_cum_cts = onco_score_cts.cumsum()
onco_score_pvals = onco_score_cum_cts / float(onco_score_cts.sum())
# tsg score
tsg_score_cts = result_df['tsg score'].value_counts()
tsg_score_cts = tsg_score_cts.sort_index(ascending=False)
tsg_score_cum_cts = tsg_score_cts.cumsum()
tsg_score_pvals = tsg_score_cum_cts / float(tsg_score_cts.sum())
# construct null p-value score distribution
score_ix = set(driver_score_pvals.index) | set(onco_score_pvals.index) | set(tsg_score_pvals.index)
score_pvals = pd.DataFrame(index=list(score_ix))
score_pvals['oncogene p-value'] = onco_score_pvals
score_pvals['tsg p-value'] = tsg_score_pvals
score_pvals['driver p-value'] = driver_score_pvals
score_pvals = score_pvals.sort_index(ascending=False)
score_pvals.to_csv(cli_opts['null_distribution'], sep='\t',
index_label='score')
else:
# do classification
pred_results_path = _utils.clf_result_dir + cfg_opts['rrand_forest_pred']
logger.info('Saving results to {0}'.format(pred_results_path))
result_df = trained_rand_forest_pred(rrclf, df, pred_results_path,
null_pvals, is_cv)
result_df.to_csv(pred_results_path, sep='\t')
# create qq plot
try:
qq_plot_path = _utils.clf_plot_dir + cfg_opts['qq_plot']
plot_data.create_qqplots(result_df, qq_plot_path)
except:
pass
logger.info('Finished classification.')
return
df = pd.read_csv(feature_path, sep='\t', index_col=0)
# R's random forest
logger.info('Running Random forest . . .')
# initialize R's random forest
rrclf = RRandomForest(df,
other_sample_ratio=cli_opts['other_ratio'],
driver_sample=cli_opts['driver_rate'],
ntrees=cli_opts['ntrees'],
seed=cli_opts['random_seed'])
# analyze classification metrics
rrclf.kfold_validation()
rrclf_onco_tpr, rrclf_onco_fpr, rrclf_onco_mean_roc_auc = rrclf.get_onco_roc_metrics()
rrclf_onco_precision, rrclf_onco_recall, rrclf_onco_mean_pr_auc = rrclf.get_onco_pr_metrics()
rrclf_tsg_tpr, rrclf_tsg_fpr, rrclf_tsg_mean_roc_auc = rrclf.get_tsg_roc_metrics()
rrclf_tsg_precision, rrclf_tsg_recall, rrclf_tsg_mean_pr_auc = rrclf.get_tsg_pr_metrics()
rrclf_driver_precision, rrclf_driver_recall, rrclf_driver_mean_pr_auc = rrclf.get_driver_pr_metrics()
rrclf_driver_tpr, rrclf_driver_fpr, rrclf_driver_mean_roc_auc = rrclf.get_driver_roc_metrics()
# skip if no matplotlib
try:
# plot feature importance
mean_df = rrclf.mean_importance
std_df = rrclf.std_importance
feat_path = _utils.clf_plot_dir + cfg_opts['r_feature_importance_plot']
plot_data.feature_importance_barplot(mean_df, std_df, feat_path)
except:
pass
# run predictions using R's random forest
pred_results_path = _utils.clf_result_dir + cfg_opts['rrand_forest_pred']
result_df = rand_forest_pred(rrclf, df, result_path=pred_results_path,
null_dist=null_pvals)
# save a list of oncogenes/tsgs in separate files
if null_pvals is None:
pred_onco = result_df[result_df['majority vote class']==_utils.onco_label].index.to_series()
novel_onco = result_df[(result_df['majority vote class']==_utils.onco_label) & (result_df['training list class']!=_utils.onco_label)].index.to_series()
pred_tsg = result_df[result_df['majority vote class']==_utils.tsg_label].index.to_series()
novel_tsg = result_df[(result_df['majority vote class']==_utils.tsg_label) & (result_df['training list class']!=_utils.tsg_label)].index.to_series()
pred_driver = result_df[result_df['majority vote cancer gene']==1].index.to_series()
pred_onco.to_csv(_utils.clf_result_dir + cfg_opts['rrf_onco'], sep='\t', index=False, header=None)
novel_onco.to_csv(_utils.clf_result_dir + cfg_opts['rrf_novel_onco'], sep='\t', index=False, header=None)
pred_tsg.to_csv(_utils.clf_result_dir + cfg_opts['rrf_tsg'], sep='\t', index=False, header=None)
novel_tsg.to_csv(_utils.clf_result_dir + cfg_opts['rrf_novel_tsg'], sep='\t', index=False, header=None)
log_str = ('Majority vote Random forest: {0} ({1} novel) oncogenes, '
'{2} ({3} novel) tsg'.format(len(pred_onco), len(novel_onco),
len(pred_tsg), len(novel_tsg)))
logger.info(log_str)
else:
pred_onco = result_df[result_df['oncogene q-value']<=.1].index.to_series()
novel_onco = result_df[(result_df['oncogene q-value']<=.1) & (result_df['training list class']!=_utils.onco_label)].index.to_series()
pred_tsg = result_df[result_df['tsg q-value']<=.1].index.to_series()
novel_tsg = result_df[(result_df['tsg q-value']<=.1) & (result_df['training list class']!=_utils.tsg_label)].index.to_series()
pred_driver = result_df[result_df['driver q-value']<=.1].index.to_series()
pred_onco.to_csv(_utils.clf_result_dir + cfg_opts['rrf_onco'], sep='\t', index=False, header=None)
novel_onco.to_csv(_utils.clf_result_dir + cfg_opts['rrf_novel_onco'], sep='\t', index=False, header=None)
pred_tsg.to_csv(_utils.clf_result_dir + cfg_opts['rrf_tsg'], sep='\t', index=False, header=None)
novel_tsg.to_csv(_utils.clf_result_dir + cfg_opts['rrf_novel_tsg'], sep='\t', index=False, header=None)
log_str = ('Random forest significance test: {0} ({1} novel) oncogenes, '
'{2} ({3} novel) tsg'.format(len(pred_onco), len(novel_onco),
len(pred_tsg), len(novel_tsg)))
logger.info(log_str)
# only plot if matplotlib
try:
# plot r random forest results
plot_data.prob_scatter(result_df,
plot_path=_utils.clf_plot_dir + cfg_opts['rrand_forest_plot'],
title='Sub-sampled Random Forest Predictions')
plot_data.prob_kde(result_df,
col_name='oncogene score',
save_path=_utils.clf_plot_dir + cfg_opts['onco_kde_rrand_forest'],
title='Distribution of Oncogene Scores (sub-sampled random forest)')
plot_data.prob_kde(result_df,
col_name='tsg score',
save_path=_utils.clf_plot_dir + cfg_opts['tsg_kde_rrand_forest'],
title='Distribution of TSG Scores (sub-sampled random forest)')
logger.info('Finished running sub-sampled Random Forest')
# dummy classifier, predict most frequent
logger.debug('Running Dummy Classifier. . .')
dclf = DummyClf(df,
strategy='most_frequent',
min_ct=minimum_ct,
weight=False)
dclf.kfold_validation()
dclf_onco_tpr, dclf_onco_fpr, dclf_onco_mean_roc_auc = dclf.get_onco_roc_metrics()
dclf_onco_precision, dclf_onco_recall, dclf_onco_mean_pr_auc = dclf.get_onco_pr_metrics()
dclf_tsg_tpr, dclf_tsg_fpr, dclf_tsg_mean_roc_auc = dclf.get_tsg_roc_metrics()
dclf_tsg_precision, dclf_tsg_recall, dclf_tsg_mean_pr_auc = dclf.get_tsg_pr_metrics()
dclf_driver_tpr, dclf_driver_fpr, dclf_driver_mean_roc_auc = dclf.get_driver_roc_metrics()
logger.debug('Finished dummy classifier.')
# plot oncogene roc figure
rrandom_forest_str = '20/20+ Classifier (AUC = %0.3f)' % rrclf_onco_mean_roc_auc
dummy_str = 'dummy (AUC = %0.3f)' % dclf_onco_mean_roc_auc
rrclf_onco_mean_tpr = np.mean(rrclf_onco_tpr, axis=0)
dclf_onco_mean_tpr = np.mean(dclf_onco_tpr, axis=0)
df = pd.DataFrame({
rrandom_forest_str: rrclf_onco_mean_tpr,
dummy_str: dclf_onco_mean_tpr},
index=rrclf_onco_fpr)
line_style = {dummy_str: '--',
rrandom_forest_str: '-',
}
save_path = _utils.clf_plot_dir + cfg_opts['roc_plot_oncogene']
plot_data.receiver_operator_curve(df, save_path, line_style)
# plot tsg roc figure
r_random_forest_str = '20/20+ Classifier (AUC = %0.3f)' % rrclf_tsg_mean_roc_auc
dummy_str = 'dummy (AUC = %0.3f)' % dclf_tsg_mean_roc_auc
rrclf_tsg_mean_tpr = np.mean(rrclf_tsg_tpr, axis=0)
dclf_tsg_mean_tpr = np.mean(dclf_tsg_tpr, axis=0)
df = pd.DataFrame({r_random_forest_str: rrclf_tsg_mean_tpr,
dummy_str: dclf_tsg_mean_tpr},
index=rrclf_tsg_fpr)
line_style = {dummy_str: '--',
r_random_forest_str: '-',
}
save_path = _utils.clf_plot_dir + cfg_opts['roc_plot_tsg']
plot_data.receiver_operator_curve(df, save_path, line_style)
# plot driver roc figure
r_random_forest_str = '20/20+ Classifier (AUC = %0.3f)' % rrclf_driver_mean_roc_auc
dummy_str = 'dummy (AUC = %0.3f)' % dclf_driver_mean_roc_auc
rrclf_driver_mean_tpr = np.mean(rrclf_driver_tpr, axis=0)
dclf_driver_mean_tpr = np.mean(dclf_driver_tpr, axis=0)
df = pd.DataFrame({r_random_forest_str: rrclf_driver_mean_tpr,
dummy_str: dclf_driver_mean_tpr},
index=rrclf_driver_fpr)
line_style = {dummy_str: '--',
r_random_forest_str: '-',
}
save_path = _utils.clf_plot_dir + cfg_opts['roc_plot_driver']
plot_data.receiver_operator_curve(df, save_path, line_style)
# plot oncogene pr figure
rrandom_forest_str = '20/20+ Classifier (AUC = %0.3f)' % rrclf_onco_mean_pr_auc
dummy_str = 'dummy (AUC = %0.3f)' % dclf_onco_mean_pr_auc
rrclf_onco_mean_precision = np.mean(rrclf_onco_precision, axis=0)
dclf_onco_mean_precision = np.mean(dclf_onco_precision, axis=0)
df = pd.DataFrame({rrandom_forest_str: rrclf_onco_mean_precision,},
index=rrclf_onco_recall)
line_style = {dummy_str: '--',
rrandom_forest_str: '-',
}
save_path = _utils.clf_plot_dir + cfg_opts['pr_plot_oncogene']
plot_data.precision_recall_curve(df, save_path, line_style,
#sem_df,
title='Oncogene Precision-Recall Curve')
# plot tsg pr figure
r_random_forest_str = '20/20+ Classifier (AUC = %0.3f)' % rrclf_tsg_mean_pr_auc
dummy_str = 'dummy (AUC = %0.3f)' % dclf_tsg_mean_pr_auc
rrclf_tsg_mean_precision = np.mean(rrclf_tsg_precision, axis=0)
dclf_tsg_mean_precision = np.mean(dclf_tsg_precision, axis=0)
df = pd.DataFrame({
r_random_forest_str: rrclf_tsg_mean_precision,
},
index=rrclf_tsg_recall)
line_style = {dummy_str: '--',
r_random_forest_str: '-',
}
save_path = _utils.clf_plot_dir + cfg_opts['pr_plot_tsg']
plot_data.precision_recall_curve(df, save_path, line_style,
title='TSG Precision-Recall Curve')
# plot driver gene pr figure
r_random_forest_str = '20/20+ Classifier (AUC = %0.3f)' % rrclf_driver_mean_pr_auc
rrclf_driver_mean_precision = np.mean(rrclf_driver_precision, axis=0)
df = pd.DataFrame({
r_random_forest_str: rrclf_driver_mean_precision,
},
index=rrclf_driver_recall)
line_style = {dummy_str: '--',
r_random_forest_str: '-',
}
save_path = _utils.clf_plot_dir + cfg_opts['pr_plot_driver']
plot_data.precision_recall_curve(df, save_path, line_style,
title='Driver Precision-Recall Curve')
# save performance metrics of ROC and PR AUC
save_path = _utils.clf_result_dir + cfg_opts['performance']
logger.info('Saving performance metrics ({0}) . . .'.format(save_path))
metrics = [['TSG', rrclf_tsg_mean_roc_auc, rrclf_tsg_mean_pr_auc],
['OG', rrclf_onco_mean_roc_auc, rrclf_onco_mean_pr_auc],
['Driver', rrclf_driver_mean_roc_auc, rrclf_driver_mean_pr_auc]]
perf_df = pd.DataFrame(metrics, columns=['Type', 'ROC AUC', 'PR AUC'])
perf_df.to_csv(save_path, sep='\t', index=False)
# make qq plot
qq_plot_path = _utils.clf_plot_dir + cfg_opts['qq_plot']
plot_data.create_qqplots(result_df, qq_plot_path)
except:
pass
def sample_boxplot(pred_onco,
pred_tsg,
pred_driver,
save_path_type,
save_path_driver,
xlabel='',
ylabel='',
title=''):
"""Create a box plot for distribution of percentage of tumor samples
containing a non-silent mutation in different categories of genes (ie
oncogenes, tsgs, and drivers).
Parameters
----------
pred_onco : list
list of genes predicted as oncogenes
pred_tsg : list
list of genes predicted as tsgs
pred_driver : list
list of genes predicted as drivers
save_path_type : str
path to save figure for comparing oncogenes, tsgs, and other
save_path_driver : str
path to save figure for comparing drivers vs other
xlabel : str
x-axis label
ylabel : str
y-axis label
title : str
title of figures
"""
cfg = _utils.get_output_config('sample')
df = pd.read_csv(_utils.result_dir + cfg['max_gene_pct_sample_out'],
sep='\t', index_col=0)
df['Predicted Type'] = [("oncogene" if g in pred_onco else "other") for g in df.index]
df.ix[pred_tsg, 'Predicted Type'] = 'TSG'
df['Predicted Driver'] = [("driver" if g in pred_driver else "other") for g in df.index]
# set figure labels
if not xlabel:
xlabel = 'Predicted Type'
if not ylabel:
ylabel = 'Maximum Pct of Samples for a Tumor Type'
if not title:
title = 'Percentage of Samples with Non-Silent Mutation'
# plot with oncogenes, tsgs, and other
myplt.boxplot(df,
by='Predicted Type',
column=['all mutation sample pct', 'non-silent sample pct'],
save_path=save_path_type,
xlabel=xlabel,
ylabel=ylabel,
title=title)
# plot with drivers vs other
myplt.boxplot(df,
by='Predicted Driver',
column=['all mutation sample pct', 'non-silent sample pct'],
save_path=save_path_driver,
xlabel=xlabel,
ylabel=ylabel,
title=title)
def retrieve_gene_features(conn, opts,
get_entropy=True):
"""Retrieve gene information from the gene_features table.
See the gene_features module to understand the gene_features
database table.
Parameters
----------
conn : mysql/sqlite connection
connection to db with gene_features table
options : dict
options for getting info
get_entropy : bool
option to togle the use of entropy features.
Since entropy features are read from a file in this function, it may
induce a not necessary dependency on previously running commands.
To avoid this, set get_entropy=False and then compute entropy features
separately.
Returns
-------
df : pd.dataframe
dataframe of gene lengths
"""
logger.info('Retrieving features of genes . . .')
selected_cols = ['gene']
# retrieve more features if specified by command line
if opts['gene_length']:
selected_cols.append('gene_length')
if opts['mutation_rate']:
selected_cols.append('noncoding_mutation_rate')
if opts['replication_time']:
selected_cols.append('replication_time')
if opts['expression']:
selected_cols.append('expression_CCLE as expression')
if opts['hic']:
selected_cols.append('HiC_compartment')
if opts['betweeness']:
selected_cols.append('gene_betweeness')
if opts['degree']:
selected_cols.append('gene_degree')
# get info from gene_features table
logger.info('Retrieving gene feature information from gene_features table . . . ')
sql = "SELECT %s FROM gene_features" % ', '.join(selected_cols)
df = psql.frame_query(sql, conn)
df = df.set_index('gene')
df['gene'] = df.index
logger.info('Finished retrieving gene features from gene_features table.')
# fill graph stats with zeros if gene not in Biogrid
if 'gene_betweeness' in df.columns:
df['gene_betweeness'] = df['gene_betweeness'].fillna(0)
if 'gene_degree' in df.columns:
df['gene_degree'] = df['gene_degree'].fillna(0)
# get position entropy features
if get_entropy:
entropy_cfg = _utils.get_output_config('position_entropy')
mutation_pos_entropy = pd.read_csv(_utils.result_dir + entropy_cfg['mutation_pos_entropy'],
sep='\t', index_col=0)
missense_pos_entropy = pd.read_csv(_utils.result_dir + entropy_cfg['missense_pos_entropy'],
sep='\t', index_col=0)
#df['mutation position entropy'] = mutation_pos_entropy['mutation position entropy']
#df['pct of uniform mutation entropy'] = mutation_pos_entropy['pct of uniform mutation entropy']
df['missense position entropy'] = missense_pos_entropy['missense position entropy']
df['pct of uniform missense entropy'] = missense_pos_entropy['pct of uniform missense entropy']
return df
