def __init__(self):
'Create a new experiment with given sample locations for water and WASTE; totalTime is expected run time in seconds, if known'
worklist.comment("Generated %s" % (datetime.now().ctime()))
worklist.userprompt(
"The following reagent tubes should be present: %s" %
Sample.getAllLocOnPlate(decklayout.REAGENTPLATE))
worklist.userprompt(
"The following eppendorf tubes should be present: %s" %
Sample.getAllLocOnPlate(decklayout.EPPENDORFS))
worklist.email(dest='*****@*****.**',
subject='Run started (Generate: %s)' %
(datetime.now().ctime()))
worklist.email(dest='*****@*****.**',
subject='Tecan error',
onerror=1)
self.cleanTips = 0
# self.sanitize() # Not needed, TRP does it, also first use of tips will do this
self.useDiTis = False
self.ptcrunning = False
self.overrideSanitize = False
self.pgmStartTime = None
self.pgmEndTime = None
# Access PTC and RIC early to be sure they are working
worklist.pyrun("PTC\\ptctest.py")
# worklist.periodicWash(15,4)
worklist.userprompt(
"Verify that PTC thermocycler lid pressure is set to '2'.")
self.idlePgms = []
self.timerStartTime = [None] * 8
def __init__(self):
'Create a new experiment with given sample locations for water and WASTE; totalTime is expected run time in seconds, if known'
self.checksum=md5sum(sys.argv[0])
self.checksum=self.checksum[-4:]
pyTecan=os.path.dirname(os.path.realpath(__file__))
self.gitlabel=strip(subprocess.check_output(["git", "describe","--always"],cwd=pyTecan))
worklist.comment("Generated %s (%s-%s pyTecan-%s)"%(datetime.now().ctime(),sys.argv[0],self.checksum,self.gitlabel))
worklist.userprompt("The following reagent tubes should be present: %s"%Sample.getAllLocOnPlate(decklayout.REAGENTPLATE))
worklist.userprompt("The following eppendorf tubes should be present: %s"%Sample.getAllLocOnPlate(decklayout.EPPENDORFS))
worklist.email(dest='*****@*****.**',subject='Run started (Generate: %s) expected runtime %.0f minutes'%(datetime.now().ctime(),clock.totalTime/60.0 if clock.totalTime is not None else 0.0 ) )
worklist.email(dest='*****@*****.**',subject='Tecan error',onerror=1)
self.cleanTips=0
# self.sanitize() # Not needed, TRP does it, also first use of tips will do this
self.useDiTis=False
self.ptcrunning=False
self.overrideSanitize=False
self.pgmStartTime=None
self.pgmEndTime=None
# Access PTC and RIC early to be sure they are working
worklist.pyrun("PTC\\ptctest.py")
# worklist.periodicWash(15,4)
worklist.userprompt("Verify that PTC thermocycler lid pressure is set to '2'.")
self.idlePgms=[]
self.timerStartTime=[None]*8
def __init__(self):
'Create a new experiment with given sample locations for water and WASTE; totalTime is expected run time in seconds, if known'
worklist.comment("Generated %s"%(datetime.now().ctime()))
worklist.userprompt("The following reagent tubes should be present: %s"%Sample.getAllLocOnPlate(decklayout.REAGENTPLATE))
worklist.userprompt("The following eppendorf tubes should be present: %s"%Sample.getAllLocOnPlate(decklayout.EPPENDORFS))
worklist.email(dest='*****@*****.**',subject='Run started (Generate: %s)'%(datetime.now().ctime()))
worklist.email(dest='*****@*****.**',subject='Tecan error',onerror=1)
self.cleanTips=0
# self.sanitize() # Not needed, TRP does it, also first use of tips will do this
self.useDiTis=False
self.ptcrunning=False
self.overrideSanitize=False
self.pgmStartTime=None
self.pgmEndTime=None
# Access PTC and RIC early to be sure they are working
worklist.pyrun("PTC\\ptctest.py")
# worklist.periodicWash(15,4)
worklist.userprompt("Verify that PTC thermocycler lid pressure is set to '2'.")
self.idlePgms=[]
self.timerStartTime=[None]*8
def __init__(self):
'Create a new experiment with given sample locations for water and WASTE; totalTime is expected run time in seconds, if known'
self.checksum = md5sum(sys.argv[0])
self.checksum = self.checksum[-4:]
pyTecan = os.path.dirname(os.path.realpath(__file__))
self.gitlabel = strip(
subprocess.check_output(["git", "describe", "--always"],
cwd=pyTecan))
worklist.comment("Generated %s (%s-%s pyTecan-%s)" %
(datetime.now().ctime(), sys.argv[0], self.checksum,
self.gitlabel))
worklist.userprompt(
"The following reagent tubes should be present: %s" %
Sample.getAllLocOnPlate(decklayout.REAGENTPLATE))
worklist.userprompt(
"The following eppendorf tubes should be present: %s" %
Sample.getAllLocOnPlate(decklayout.EPPENDORFS))
worklist.email(
dest='*****@*****.**',
subject='Run started (Generate: %s) expected runtime %.0f minutes'
% (datetime.now().ctime(),
clock.totalTime / 60.0 if clock.totalTime is not None else 0.0))
worklist.email(dest='*****@*****.**',
subject='Tecan error',
onerror=1)
self.cleanTips = 0
# self.sanitize() # Not needed, TRP does it, also first use of tips will do this
self.useDiTis = False
self.ptcrunning = False
self.overrideSanitize = False
self.pgmStartTime = None
self.pgmEndTime = None
# Access PTC and RIC early to be sure they are working
worklist.pyrun("PTC\\ptctest.py")
# worklist.periodicWash(15,4)
worklist.userprompt(
"Verify that PTC thermocycler lid pressure is set to '2'.")
self.idlePgms = []
self.timerStartTime = [None] * 8
import worklist
import plate
#aspirate(1,"LC",[1,3,4],1,2,3,"wells")
plate1=plate.Plate("Samples",4,3,12,8)
worklist.aspirate(7,['B3','D3','E3'],"Water",[10,20,30],plate1)
worklist.aspirate(7,['B4','D4','F4'],"Water",[10,20,30],plate1)
worklist.aspirate(1,['B1'],"Water",[10],plate1)
worklist.dispense(2,['B1'],"Water",[10],plate1)
worklist.mix(2,['B1'],"Water",[10],plate1,7)
worklist.vector("ROMAVector",plate1,worklist.SAFETOEND,True,worklist.OPEN,worklist.CLOSE)
worklist.execute("python test",True,"result")
worklist.userprompt("Script done")
worklist.dump()
import worklist
import plate
#aspirate(1,"LC",[1,3,4],1,2,3,"wells")
plate1 = plate.Plate("Samples", 4, 3, 12, 8)
worklist.aspirate(7, ['B3', 'D3', 'E3'], "Water", [10, 20, 30], plate1)
worklist.aspirate(7, ['B4', 'D4', 'F4'], "Water", [10, 20, 30], plate1)
worklist.aspirate(1, ['B1'], "Water", [10], plate1)
worklist.dispense(2, ['B1'], "Water", [10], plate1)
worklist.mix(2, ['B1'], "Water", [10], plate1, 7)
worklist.vector("ROMAVector", plate1, worklist.SAFETOEND, True, worklist.OPEN,
worklist.CLOSE)
worklist.execute("python test", True, "result")
worklist.userprompt("Script done")
worklist.dump()
