Exemplo n.º 1
0
def run(fname_recording, recording_dtype, fname_spike_train,
        output_directory):
           
    """
    """

    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    # make output directory if not exist
    if not os.path.exists(output_directory):
        os.mkdir(output_directory)

    # get reader
    reader = READER(fname_recording,
                    recording_dtype,
                    CONFIG)
    reader.spike_size = CONFIG.spike_size_nn

    # get noise covariance
    logger.info('Compute Noise Covaraince')
    save_dir = os.path.join(output_directory, 'noise_cov')
    chunk = [0, np.min((5*60*reader.sampling_rate, reader.end))]
    fname_spatial_sig, fname_temporal_sig = get_noise_covariance(
        reader, save_dir, CONFIG, chunk)
    
    # get processed templates
    logger.info('Crop Templates')
    save_dir = os.path.join(output_directory, 'templates')
    fname_templates_snippets = get_templates_on_local_channels(
        reader, save_dir, fname_spike_train, CONFIG)

    # denoise templates
    fname_templates_denoised = denoise_templates(
        fname_templates_snippets, save_dir)

    # make training data
    logger.info('Make Training Data')
    DetectTD = Detection_Training_Data(
        fname_templates_denoised,
        fname_spatial_sig,
        fname_temporal_sig)
    
    DenoTD = Denoising_Training_Data(
        fname_templates_denoised,
        fname_spatial_sig,
        fname_temporal_sig)
    
    return DetectTD, DenoTD
Exemplo n.º 2
0
Arquivo: run.py Projeto: AkiHase/yass
def residual_ONgpu(recordings_filename, recording_dtype, CONFIG, fname_shifts,
                   fname_templates, output_directory, dtype_out, fname_out,
                   fname_spike_train, update_templates, run_chunk_sec):

    # get data reader
    if run_chunk_sec == 'full':
        chunk_sec = None
    else:
        chunk_sec = run_chunk_sec

    reader = READER(recordings_filename,
                    recording_dtype,
                    CONFIG,
                    CONFIG.resources.n_sec_chunk_gpu_deconv,
                    chunk_sec=chunk_sec)

    if False:
        RESIDUAL_GPU3(reader, recordings_filename, recording_dtype, CONFIG,
                      fname_shifts, fname_templates, output_directory,
                      dtype_out, fname_out, fname_spike_train,
                      update_templates)
    else:
        RESIDUAL_GPU2(reader, recordings_filename, recording_dtype, CONFIG,
                      fname_shifts, fname_templates, output_directory,
                      dtype_out, fname_out, fname_spike_train,
                      update_templates)
Exemplo n.º 3
0
Arquivo: run.py Projeto: AkiHase/yass
def residual_ONcpu(fname_templates, fname_spike_train, output_directory,
                   recordings_filename, recording_dtype, dtype_out, fname_out,
                   run_chunk_sec, CONFIG):

    # get data reader
    if run_chunk_sec == 'full':
        chunk_sec = None
    else:
        chunk_sec = run_chunk_sec

    reader = READER(recordings_filename,
                    recording_dtype,
                    CONFIG,
                    CONFIG.resources.n_sec_chunk,
                    chunk_sec=chunk_sec)

    # get residual object
    residual_object = RESIDUAL(fname_templates, fname_spike_train, reader,
                               fname_out, dtype_out)

    # compute residual
    seg_dir = os.path.join(output_directory, 'segs')
    residual_object.compute_residual(seg_dir,
                                     CONFIG.resources.multi_processing,
                                     CONFIG.resources.n_processors)

    # concatenate all segments
    residual_object.save_residual()
Exemplo n.º 4
0
def run_neural_network(standardized_path,
                       standardized_dtype,
                       output_directory,
                       run_chunk_sec='full'):
    """Run neural network detection
    """
    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    # load NN detector
    detector = Detect(CONFIG.neuralnetwork.detect.n_filters,
                      CONFIG.spike_size_nn, CONFIG.channel_index)
    detector.load(CONFIG.neuralnetwork.detect.filename)

    # load NN denoiser
    denoiser = Denoise(CONFIG.neuralnetwork.denoise.n_filters,
                       CONFIG.neuralnetwork.denoise.filter_sizes,
                       CONFIG.spike_size_nn)
    denoiser.load(CONFIG.neuralnetwork.denoise.filename)

    # get data reader
    batch_length = CONFIG.resources.n_sec_chunk * CONFIG.resources.n_processors
    n_sec_chunk = CONFIG.resources.n_sec_chunk_gpu_detect
    print("   batch length to (sec): ", batch_length,
          " (longer increase speed a bit)")
    print("   length of each seg (sec): ", n_sec_chunk)
    buffer = CONFIG.spike_size_nn
    if run_chunk_sec == 'full':
        chunk_sec = None
    else:
        chunk_sec = run_chunk_sec

    reader = READER(standardized_path, standardized_dtype, CONFIG,
                    batch_length, buffer, chunk_sec)

    # neighboring channels
    channel_index_dedup = make_channel_index(CONFIG.neigh_channels,
                                             CONFIG.geom,
                                             steps=2)

    # threshold for neuralnet detection
    detect_threshold = CONFIG.detect.threshold

    # loop over each chunk
    batch_ids = np.arange(reader.n_batches)
    batch_ids_split = np.split(batch_ids, len(CONFIG.torch_devices))
    processes = []
    for ii, device in enumerate(CONFIG.torch_devices):
        p = mp.Process(target=run_nn_detction_batch,
                       args=(batch_ids_split[ii], output_directory, reader,
                             n_sec_chunk, detector, denoiser,
                             channel_index_dedup, detect_threshold, device))
        p.start()
        processes.append(p)
    for p in processes:
        p.join()
Exemplo n.º 5
0
def get_plot_ptps(save_dir,
                  fname_raw,
                  fname_residual,
                  fname_spike_train,
                  fname_scales,
                  fname_shifts,
                  templates_dir,
                  ptp_threshold,
                  n_col,
                  CONFIG,
                  units_in=None,
                  fname_drifts_gt=None,
                  n_nearby_units=3):

    reader_raw = READER(fname_raw, 'float32', CONFIG)
    reader_resid = READER(fname_residual, 'float32', CONFIG)
    update_time = CONFIG.deconvolution.template_update_time

    # load initial templates
    init_templates = np.load(
        os.path.join(templates_dir, 'templates_{}sec.npy').format(0))
    n_units = init_templates.shape[0]

    meta_data_dir = os.path.join(save_dir, 'meta_data')
    if not os.path.exists(meta_data_dir):
        os.makedirs(meta_data_dir)

    figs_dir = os.path.join(save_dir, 'figs')
    if not os.path.exists(figs_dir):
        os.makedirs(figs_dir)

    if units_in is None:
        units_in = np.arange(n_units)
    units_in = units_in[units_in < n_units]

    get_plot_ptps_parallel(units_in, reader_raw, reader_resid,
                           fname_spike_train, fname_scales, fname_shifts,
                           templates_dir, meta_data_dir, figs_dir, update_time,
                           ptp_threshold, n_col, fname_drifts_gt,
                           n_nearby_units)
Exemplo n.º 6
0
def run_template_update(output_directory,
                        fname_templates,
                        fname_spike_train,
                        fname_shifts,
                        fname_scales,
                        fname_residual,
                        residual_dtype,
                        residual_offset=0,
                        update_weight=50,
                        units_to_update=None):

    fname_templates_out = os.path.join(output_directory, 'templates.npy')

    if not os.path.exists(fname_templates_out):

        print('updating templates')

        CONFIG = read_config()

        # output folder
        if not os.path.exists(output_directory):
            os.makedirs(output_directory)

        # reader
        if CONFIG.deconvolution.deconv_gpu:
            n_sec_chunk = CONFIG.resources.n_sec_chunk_gpu_deconv
        else:
            n_sec_chunk = CONFIG.resources.n_sec_chunk
        reader_residual = READER(fname_residual,
                                 residual_dtype,
                                 CONFIG,
                                 n_sec_chunk,
                                 offset=residual_offset)

        # residual obj that can shift templates in gpu
        residual_comp = RESIDUAL_GPU2(None, CONFIG, None, None, None, None,
                                      None, None, None, None, True)
        residual_comp.load_templates(fname_templates)
        residual_comp.make_bsplines_parallel()

        avg_min_max_vals, weights = get_avg_min_max_vals(
            fname_templates, fname_spike_train, fname_shifts, fname_scales,
            reader_residual, residual_comp, units_to_update)

        templates_updated = update_templates(fname_templates, weights,
                                             avg_min_max_vals, update_weight,
                                             units_to_update)

        np.save(fname_templates_out, templates_updated)

    return fname_templates_out
Exemplo n.º 7
0
def run_voltage_treshold(standardized_path,
                         standardized_dtype,
                         output_directory,
                         run_chunk_sec='full'):
    """Run detection that thresholds on amplitude
    """
    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    # get data reader
    #n_sec_chunk = CONFIG.resources.n_sec_chunk*CONFIG.resources.n_processors
    batch_length = CONFIG.resources.n_sec_chunk
    n_sec_chunk = 0.5
    print("   batch length to (sec): ", batch_length,
          " (longer increase speed a bit)")
    print("   length of each seg (sec): ", n_sec_chunk)
    buffer = CONFIG.spike_size
    if run_chunk_sec == 'full':
        chunk_sec = None
    else:
        chunk_sec = run_chunk_sec

    reader = READER(standardized_path, standardized_dtype, CONFIG,
                    batch_length, buffer, chunk_sec)

    # number of processed chunks
    n_mini_per_big_batch = int(np.ceil(batch_length / n_sec_chunk))
    total_processing = int(reader.n_batches * n_mini_per_big_batch)

    # neighboring channels
    channel_index = make_channel_index(CONFIG.neigh_channels,
                                       CONFIG.geom,
                                       steps=2)

    if CONFIG.resources.multi_processing:
        parmap.starmap(run_voltage_threshold_parallel,
                       list(zip(np.arange(reader.n_batches))),
                       reader,
                       n_sec_chunk,
                       CONFIG.detect.threshold,
                       channel_index,
                       output_directory,
                       processes=CONFIG.resources.n_processors,
                       pm_pbar=True)
    else:
        for batch_id in range(reader.n_batches):
            run_voltage_threshold_parallel(batch_id, reader, n_sec_chunk,
                                           CONFIG.detect.threshold,
                                           channel_index, output_directory)
Exemplo n.º 8
0
Arquivo: run.py Projeto: AkiHase/yass
def run(template_fname, spike_train_fname, shifts_fname, output_directory,
        residual_fname, residual_dtype):

    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    #
    fname_out = os.path.join(output_directory, 'soft_assignment.npy')
    if os.path.exists(fname_out):
        return fname_out

    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    # reader for residual
    reader_resid = READER(residual_fname, residual_dtype, CONFIG,
                          CONFIG.resources.n_sec_chunk_gpu_deconv)

    # load NN detector
    detector = Detect(CONFIG.neuralnetwork.detect.n_filters,
                      CONFIG.spike_size_nn, CONFIG.channel_index)
    detector.load(CONFIG.neuralnetwork.detect.filename)
    detector = detector.cuda()

    # initialize soft assignment calculator
    threshold = CONFIG.deconvolution.threshold / 0.1
    sna = SOFTNOISEASSIGNMENT(spike_train_fname, template_fname, shifts_fname,
                              reader_resid, detector, CONFIG.channel_index,
                              threshold)

    # compuate soft assignment
    probs = sna.compute_soft_assignment()
    np.save(fname_out, probs)

    return fname_out
Exemplo n.º 9
0
def run(output_directory):
    """Preprocess pipeline: filtering, standarization and whitening filter

    This step (optionally) performs filtering on the data, standarizes it
    and computes a whitening filter. Filtering and standardized data are
    processed in chunks and written to disk.

    Parameters
    ----------
    output_directory: str
        where results will be saved

    Returns
    -------
    standardized_path: str
        Path to standardized data binary file

    standardized_params: str
        Path to standardized data parameters

    channel_index: numpy.ndarray
        Channel indexes

    whiten_filter: numpy.ndarray
        Whiten matrix

    Notes
    -----
    Running the preprocessor will generate the followiing files in
    CONFIG.data.root_folder/output_directory/:

    * ``filtered.bin`` - Filtered recordings
    * ``filtered.yaml`` - Filtered recordings metadata
    * ``standardized.bin`` - Standarized recordings
    * ``standardized.yaml`` - Standarized recordings metadata
    * ``whitening.npy`` - Whitening filter

    Everything is run on CPU.

    Examples
    --------

    .. literalinclude:: ../../examples/pipeline/preprocess.py
    """

    # **********************************************
    # *********** Initialize ***********************
    # **********************************************

    logger = logging.getLogger(__name__)

    # load config
    CONFIG = read_config()

    # raw data info
    filename_raw = os.path.join(CONFIG.data.root_folder,
                                CONFIG.data.recordings)
    dtype_raw = CONFIG.recordings.dtype
    n_channels = CONFIG.recordings.n_channels

    if not CONFIG.preprocess.apply_filter:
        return filename_raw, dtype_raw

    # if apply filter, get recording reader
    n_sec_chunk = CONFIG.resources.n_sec_chunk
    reader = READER(filename_raw, dtype_raw, CONFIG, n_sec_chunk)
    logger.info("# of chunks: {}".format(reader.n_batches))

    # make output directory
    if not os.path.exists(output_directory):
        logger.info('Creating temporary folder: {}'.format(output_directory))
        os.makedirs(output_directory)
    else:
        logger.info('Temporary folder {} already exists, output will be '
                    'stored there'.format(output_directory))

    # make output parameters
    standardized_path = os.path.join(output_directory, "standardized.bin")
    standardized_params = dict(dtype=CONFIG.preprocess.dtype,
                               n_channels=n_channels)
    logger.info('Output dtype for transformed data will be {}'.format(
        CONFIG.preprocess.dtype))

    # Check if data already saved to disk and skip:
    if os.path.exists(standardized_path):
        return standardized_path, standardized_params['dtype']

    # **********************************************
    # *********** run filter & stdarize  ***********
    # **********************************************

    # get necessary parameters
    low_frequency = CONFIG.preprocess.filter.low_pass_freq
    high_factor = CONFIG.preprocess.filter.high_factor
    order = CONFIG.preprocess.filter.order
    sampling_rate = CONFIG.recordings.sampling_rate

    # estimate std from a small chunk
    chunk_5sec = 5 * CONFIG.recordings.sampling_rate
    if CONFIG.rec_len < chunk_5sec:
        chunk_5sec = CONFIG.rec_len
    small_batch = reader.read_data(
        data_start=CONFIG.rec_len // 2 - chunk_5sec // 2,
        data_end=CONFIG.rec_len // 2 + chunk_5sec // 2)

    fname_mean_sd = os.path.join(output_directory,
                                 'mean_and_standard_dev_value.npz')
    if not os.path.exists(fname_mean_sd):
        get_std(small_batch, sampling_rate, fname_mean_sd,
                CONFIG.preprocess.apply_filter, low_frequency, high_factor,
                order)
    # turn it off
    small_batch = None

    # Make directory to hold filtered batch files:
    filtered_location = os.path.join(output_directory, "filtered_files")
    if not os.path.exists(filtered_location):
        os.makedirs(filtered_location)

    # read config params
    multi_processing = CONFIG.resources.multi_processing
    if CONFIG.resources.multi_processing:
        n_processors = CONFIG.resources.n_processors
        parmap.map(filter_standardize_batch,
                   [i for i in range(reader.n_batches)],
                   reader,
                   fname_mean_sd,
                   CONFIG.preprocess.apply_filter,
                   CONFIG.preprocess.dtype,
                   filtered_location,
                   low_frequency,
                   high_factor,
                   order,
                   sampling_rate,
                   processes=n_processors,
                   pm_pbar=True)
    else:
        for batch_id in range(reader.n_batches):
            filter_standardize_batch(
                batch_id,
                reader,
                fname_mean_sd,
                CONFIG.preprocess.apply_filter,
                CONFIG.preprocess.dtype,
                filtered_location,
                low_frequency,
                high_factor,
                order,
                sampling_rate,
            )

    # Merge the chunk filtered files and delete the individual chunks
    merge_filtered_files(filtered_location, output_directory)

    # save yaml file with params
    path_to_yaml = standardized_path.replace('.bin', '.yaml')
    with open(path_to_yaml, 'w') as f:
        logger.info('Saving params...')
        yaml.dump(standardized_params, f)

    return standardized_path, standardized_params['dtype']
Exemplo n.º 10
0
def post_process(output_directory, fname_templates, fname_spike_train,
                 fname_weights, fname_recording, recording_dtype, units_in,
                 method, ctr):
    ''' 
    Run a single post process
    method: strings.
        Options are 'low_ptp', 'duplicate', 'collision',
        'high_mad', 'low_fr', 'high_fr', 'off_center',
        'duplicate_l2'
    '''

    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    if method == 'low_ptp':

        # Cat: TODO: move parameter to CONFIG
        threshold = CONFIG.clean_up.min_ptp

        # load templates
        templates = np.load(fname_templates)

        # remove low ptp
        units_out = remove_small_units(templates, threshold, units_in)

        logger.info("{} units after removing low ptp units".format(
            len(units_out)))

    elif method == 'off_center':

        threshold = CONFIG.clean_up.off_center

        # load templates
        templates = np.load(fname_templates)

        # remove off centered units
        units_out = remove_off_centered_units(templates, threshold, units_in)

        logger.info("{} units after removing off centered units".format(
            len(units_out)))

    elif method == 'duplicate':

        # tmp saving dir
        save_dir = os.path.join(output_directory, 'duplicates_{}'.format(ctr))

        # remove duplicates
        units_out = remove_duplicates(fname_templates, fname_weights, save_dir,
                                      CONFIG, units_in,
                                      CONFIG.resources.multi_processing,
                                      CONFIG.resources.n_processors)

        logger.info("{} units after removing duplicate units".format(
            len(units_out)))

    elif method == 'duplicate_l2':

        # tmp saving dir
        save_dir = os.path.join(output_directory,
                                'duplicates_l2_{}'.format(ctr))

        # remove duplicates
        n_spikes_big = 100
        min_ptp = 2
        units_out = duplicate_l2(fname_templates, fname_spike_train,
                                 CONFIG.neigh_channels, save_dir, n_spikes_big,
                                 min_ptp, units_in)

        logger.info("{} units after removing L2 duplicate units".format(
            len(units_out)))

    elif method == 'collision':
        # save folder
        save_dir = os.path.join(output_directory, 'collision_{}'.format(ctr))

        # find collision units and remove
        units_out = remove_collision(fname_templates, save_dir, CONFIG,
                                     units_in,
                                     CONFIG.resources.multi_processing,
                                     CONFIG.resources.n_processors)

        logger.info("{} units after removing collision units".format(
            len(units_out)))

    elif method == 'high_mad':

        # get data reader
        reader = READER(fname_recording, recording_dtype, CONFIG)

        # save folder
        save_dir = os.path.join(output_directory, 'mad_{}'.format(ctr))

        # neighboring channels
        neigh_channels = n_steps_neigh_channels(CONFIG.neigh_channels, 2)

        max_violations = CONFIG.clean_up.mad.max_violations
        min_var_gap = CONFIG.clean_up.mad.min_var_gap

        # find high mad units and remove
        units_out = remove_high_mad(fname_templates, fname_spike_train,
                                    fname_weights, reader, neigh_channels,
                                    save_dir, min_var_gap, max_violations,
                                    units_in,
                                    CONFIG.resources.multi_processing,
                                    CONFIG.resources.n_processors)

        logger.info("{} units after removing high mad units".format(
            len(units_out)))

    elif method == 'low_fr':

        threshold = CONFIG.clean_up.min_fr

        # length of recording in seconds
        rec_len = np.load(fname_spike_train)[:, 0].ptp()
        rec_len_sec = float(rec_len) / CONFIG.recordings.sampling_rate

        # load templates
        weights = np.load(fname_weights)

        # remove low ptp
        units_out = remove_low_fr_units(weights, rec_len_sec, threshold,
                                        units_in)

        logger.info("{} units after removing low fr units".format(
            len(units_out)))

    elif method == 'high_fr':

        # TODO: move parameter to config?
        threshold = 70

        # length of recording in seconds
        rec_len = np.load(fname_spike_train)[:, 0].ptp()
        rec_len_sec = float(rec_len) / CONFIG.recordings.sampling_rate

        # load templates
        weights = np.load(fname_weights)

        # remove low ptp
        units_out = remove_high_fr_units(weights, rec_len_sec, threshold,
                                         units_in)

        logger.info("{} units after removing high fr units".format(
            len(units_out)))

    else:
        units_out = np.copy(units_in)
        logger.info("Method not recognized. Nothing removed")

    return units_out
Exemplo n.º 11
0
def run_post_deconv_split(output_directory,
                          fname_templates,
                          fname_spike_train,
                          fname_shifts,
                          fname_scales,
                          fname_raw,
                          raw_dtype,
                          fname_residual,
                          residual_dtype,
                          residual_offset=0,
                          initial_batch=False):

    CONFIG = read_config()
    
    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    # reader
    if CONFIG.deconvolution.deconv_gpu:
        n_sec_chunk = CONFIG.resources.n_sec_chunk_gpu_deconv
    else:
        n_sec_chunk = CONFIG.resources.n_sec_chunk
    reader_residual = READER(fname_residual,
                             residual_dtype,
                             CONFIG,
                             n_sec_chunk,
                             offset=residual_offset)

    reader_raw = READER(fname_raw,
                        raw_dtype,
                        CONFIG)

    # load input data
    templates = np.load(fname_templates)
    spike_train = np.load(fname_spike_train)
    shifts = np.load(fname_shifts)
    scales = np.load(fname_scales)

    # get cleaned ptp
    fname_cleaned_ptp = os.path.join(output_directory, 'cleaned_ptp.npy')
    fname_spike_times = os.path.join(output_directory, 'spike_times.npy')
    fname_shifts = os.path.join(output_directory, 'shifts_list.npy')
    fname_scales = os.path.join(output_directory, 'scales_list.npy')
    fname_vis_chans = os.path.join(output_directory, 'vis_chans.npy')
    if os.path.exists(fname_cleaned_ptp) and os.path.exists(fname_spike_times):
        
        cleaned_ptp = np.load(fname_cleaned_ptp, allow_pickle=True)
        spike_times_list = np.load(fname_spike_times, allow_pickle=True)
        shifts_list = np.load(fname_shifts, allow_pickle=True)
        scales_list = np.load(fname_scales, allow_pickle=True)
        vis_chans = np.load(fname_vis_chans, allow_pickle=True)
    else:
        print('get cleaned ptp')
        (cleaned_ptp, spike_times_list,
         shifts_list, scales_list, vis_chans) = get_cleaned_ptp(
            templates, spike_train, shifts, scales,
            reader_residual, fname_templates, CONFIG)

        np.save(fname_shifts, shifts_list, allow_pickle=True)
        np.save(fname_scales, scales_list, allow_pickle=True)
        np.save(fname_vis_chans, vis_chans, allow_pickle=True)
        np.save(fname_cleaned_ptp, cleaned_ptp, allow_pickle=True)
        np.save(fname_spike_times, spike_times_list, allow_pickle=True)

    # split units
    fname_templates_updated = os.path.join(
        output_directory, 'templated_updated.npy')
    fname_spike_train_updated = os.path.join(
        output_directory, 'spike_train_updated.npy')
    fname_shifts_updated = os.path.join(
        output_directory, 'shifts_updated.npy')
    fname_scales_updated = os.path.join(
        output_directory, 'scales_updated.npy')
    if not(os.path.exists(fname_templates_updated) and 
           os.path.exists(fname_spike_train_updated)):

        print('run split')
        if initial_batch:
            update_original_templates = True
            min_fr_accept = 0
            min_ptp_accept = 0
            min_fraction_accept = 0
        else:
            update_original_templates = False
            min_fr_accept = 0.5
            min_ptp_accept = 10000
            min_fraction_accept = 0.15
        (templates_updated, spike_train_updated,
         shifts_updated, scales_updated) = run_split(
            cleaned_ptp, spike_times_list,
            shifts_list, scales_list, vis_chans,
            templates, spike_train, reader_raw,
            CONFIG,
            update_original_templates=update_original_templates,
            min_ptp_accept=min_ptp_accept,
            min_fr_accept=min_fr_accept,
            min_fraction_accept=min_fraction_accept)

        # denoise split units
        n_splits = templates_updated.shape[0] - templates.shape[0]
        vis_threshold_strong = 1.
        vis_threshold_weak = 0.5
        rank = 5
        pad_len = int(1.5 * CONFIG.recordings.sampling_rate / 1000.)
        jitter_len = pad_len
        split_templates_denoised = shift_svd_denoise(
            templates_updated[-n_splits:], CONFIG,
            vis_threshold_strong, vis_threshold_weak,
            rank, pad_len, jitter_len)
        templates_updated[-n_splits:] = split_templates_denoised

        ## add new templates and spike train to the existing one
        #templates_updated = np.concatenate((templates, new_temps), axis=0)
        #spike_train_new[:, 1] += templates.shape[0]
        #spike_train_updated = np.concatenate((spike_train, spike_train_new), axis=0)
        idx_sort = np.argsort(spike_train_updated[:, 0])
        spike_train_updated = spike_train_updated[idx_sort]
        shifts_updated = shifts_updated[idx_sort]
        scales_updated = scales_updated[idx_sort]
        
        np.save(fname_templates_updated, templates_updated)
        np.save(fname_spike_train_updated, spike_train_updated)
        np.save(fname_shifts_updated, shifts_updated)
        np.save(fname_scales_updated, scales_updated)

        # can be used to find gpu memory not freed
        # import gc
        #n_objects = 0
        #for obj in gc.get_objects():
        #    try:
        #        if torch.is_tensor(obj) or (hasattr(obj, 'data') and torch.is_tensor(obj.data)):
        #            print(obj, type(obj), obj.size())
        #
        #            n_objects += 1
        #    except:
        #        pass
        #print(n_objects)

        #units_to_process = np.arange(templates.shape[0], templates_updated.shape[0])

    #else:
    #    templates_updated = np.load(fname_templates_updated)
    #    units_to_process = np.arange(templates.shape[0], templates_updated.shape[0])

    ## kill duplicate templates
    #methods = ['low_ptp', 'duplicate']
    #(fname_templates_out, fname_spike_train_out, 
    # _, _, _)  = postprocess.run(
    #    methods,
    #    os.path.join(output_directory,
    #                 'duplicate_remove'),
    #    None,
    #    None,
    #    fname_templates_updated,
    #    fname_spike_train_updated,
    #    None,
    #    None,
    #    None,
    #    None,
    #    units_to_process)

    #return fname_templates_out, fname_spike_train_out

    return (fname_templates_updated, fname_spike_train_updated,
            fname_shifts_updated, fname_scales_updated)
Exemplo n.º 12
0
def run(template_fname,
        spike_train_fname,
        shifts_fname,
        scales_fname,
        output_directory,
        residual_fname,
        residual_dtype,
        residual_offset=0,
        compute_noise_soft=True,
        compute_template_soft=True,
        update_templates=False,
        similar_array=None):

    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    #
    fname_noise_soft = os.path.join(output_directory,
                                    'noise_soft_assignment.npy')
    fname_template_soft = os.path.join(output_directory,
                                       'template_soft_assignment.npz')

    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    # reader for residual
    reader_resid = READER(residual_fname,
                          residual_dtype,
                          CONFIG,
                          CONFIG.resources.n_sec_chunk_gpu_deconv,
                          offset=residual_offset)

    ########################
    # Noise soft assignment#
    ########################

    if compute_noise_soft and (not os.path.exists(fname_noise_soft)):

        if CONFIG.neuralnetwork.apply_nn:

            # load NN detector
            detector = Detect(CONFIG.neuralnetwork.detect.n_filters,
                              CONFIG.spike_size_nn, CONFIG.channel_index,
                              CONFIG)
            detector.load(CONFIG.neuralnetwork.detect.filename)
            detector = detector.cuda()

            # initialize soft assignment calculator
            threshold = CONFIG.deconvolution.threshold / 0.1

            # HACK now.. it needs a proper fix later
            if update_templates:
                template_fname_ = os.path.join(template_fname,
                                               'templates_init.npy')
            else:
                template_fname_ = template_fname
            sna = SOFTNOISEASSIGNMENT(spike_train_fname, template_fname_,
                                      shifts_fname, scales_fname, reader_resid,
                                      detector, CONFIG.channel_index,
                                      threshold)

            # compuate soft assignment
            probs_noise = sna.compute_soft_assignment()
            np.save(fname_noise_soft, probs_noise)

            del sna
            del detector

            torch.cuda.empty_cache()

        else:

            spike_train = np.load(spike_train_fname)
            np.save(fname_noise_soft, np.ones(len(spike_train)))

    ###########################
    # Template soft assignment#
    ###########################

    if compute_template_soft and (not os.path.exists(fname_template_soft)):

        # get whitening filters
        fname_spatial_cov = os.path.join(output_directory, 'spatial_cov.npy')
        fname_temporal_cov = os.path.join(output_directory, 'temporal_cov.npy')
        if not (os.path.exists(fname_spatial_cov)
                and os.path.exists(fname_temporal_cov)):
            spatial_cov, temporal_cov = get_noise_covariance(
                reader_resid, CONFIG)
            np.save(fname_spatial_cov, spatial_cov)
            np.save(fname_temporal_cov, temporal_cov)
        else:
            spatial_cov = np.load(fname_spatial_cov)
            temporal_cov = np.load(fname_temporal_cov)
        window_size = 51
        n_chans = 10
        reader_resid = READER(residual_fname,
                              residual_dtype,
                              CONFIG,
                              CONFIG.resources.n_sec_chunk_gpu_deconv,
                              offset=residual_offset)

        TAO = TEMPLATE_ASSIGN_OBJECT(
            fname_spike_train=spike_train_fname,
            fname_templates=template_fname,
            fname_shifts=shifts_fname,
            reader_residual=reader_resid,
            spat_cov=spatial_cov,
            temp_cov=temporal_cov,
            channel_idx=CONFIG.channel_index,
            geom=CONFIG.geom,
            large_unit_threshold=100000,
            n_chans=n_chans,
            rec_chans=CONFIG.channel_index.shape[0],
            sim_units=3,
            temp_thresh=5,
            lik_window=window_size,
            similar_array=similar_array,
            update_templates=update_templates,
            template_update_time=CONFIG.deconvolution.template_update_time)

        probs_templates, _, logprobs_outliers, units_assignment = TAO.run()
        #outlier spike times/units
        chi2_df = (2 * (window_size // 2) + 1) * n_chans
        cut_off = chi2(chi2_df).ppf(.999)

        #s_table = s_score(_)
        #s_table = s_score(probs_templates)
        #logprobs_outliers = logprobs_outliers/chi2_df

        cpu_sps = TAO.spike_train_og
        outliers = cpu_sps[np.where(logprobs_outliers.min(1) > cut_off)[0], :]

        #append log_probs to spike_times
        #logprobs = np.concatenate((cpu_sps,TAO.log_probs), axis = 1)
        # compuate soft assignment
        #np.save(prob_template_fname, probs_templates)
        #np.save(outlier_fname, outliers)
        #np.save(logprobs_outlier_fname, logprobs_outliers)
        #np.save(units_assign_fname, units_assignment)

        np.savez(
            fname_template_soft,
            probs_templates=probs_templates,
            units_assignment=units_assignment,
            #logprobs = _,
            #sihoulette_score = s_table,
            logprobs_outliers=logprobs_outliers,
            outliers=outliers)

        del TAO
        torch.cuda.empty_cache()

    return fname_noise_soft, fname_template_soft
Exemplo n.º 13
0
def update_templates(fname_templates,
                     fname_spike_train,
                     recordings_filename,
                     recording_dtype,
                     output_directory,
                     rate=0.002,
                     unit_ids=None):

    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    fname_templates_updated = os.path.join(output_directory,
                                           'templates_updated.npy')
    if os.path.exists(fname_templates_updated):
        return fname_templates_updated, None

    reader = READER(recordings_filename, recording_dtype, CONFIG)

    # max channel for each unit
    max_channels = np.load(fname_templates).ptp(1).argmax(1)
    fname_templates_new = run_template_computation(
        fname_spike_train,
        reader,
        output_directory,
        max_channels=max_channels,
        unit_ids=unit_ids,
        multi_processing=CONFIG.resources.multi_processing,
        n_processors=CONFIG.resources.n_processors)

    # load templates
    templates_orig = np.load(fname_templates)
    templates_new = np.load(fname_templates_new)

    n_units, n_times, n_channels = templates_orig.shape
    n_units_new = templates_new.shape[0]

    if unit_ids is None:
        unit_ids = np.arange(n_units)

    # if last few units have no spikes deconvovled, the length of new templates
    # can be shorter. then, zero pad it
    if n_units_new < n_units:
        zero_pad = np.zeros((n_units - n_units_new, n_times, n_channels),
                            'float32')
        templates_new = np.concatenate((templates_new, zero_pad), axis=0)

    # number of deconvolved spikes
    n_spikes = np.zeros(n_units)
    units_unique, n_spikes_unique = np.unique(np.load(fname_spike_train)[:, 1],
                                              return_counts=True)
    n_spikes[units_unique] = n_spikes_unique

    # update rule if it will be updated
    weight_to_update = np.power((1 - rate), n_spikes)

    # only update for units in unit_ids
    weight = np.ones(n_units)
    weight[unit_ids] = weight_to_update[unit_ids]
    weight = weight[:, None, None]

    # align templates
    templates_orig, templates_new = align_two_set_of_templates(
        templates_orig, templates_new)

    # update and save
    templates_updated = weight * templates_orig + (1 - weight) * templates_new
    np.save(fname_templates_updated, templates_updated)

    # check the difference
    max_diff = np.zeros(n_units)
    max_diff[unit_ids] = np.max(np.abs(templates_new[unit_ids] -
                                       templates_orig[unit_ids]),
                                axis=(1, 2))
    max_diff = max_diff / templates_orig.ptp(1).max(1)

    return fname_templates_updated, max_diff
Exemplo n.º 14
0
def deconv_ONcpu(fname_templates_in, output_directory, recordings_filename,
                 recording_dtype, threshold, run_chunk_sec, save_up_data,
                 fname_spike_train, fname_spike_train_up, fname_templates,
                 fname_templates_up, CONFIG):

    logger = logging.getLogger(__name__)

    # parameters
    # TODO: read from CONFIG
    if threshold is None:
        threshold = CONFIG.deconvolution.threshold
    elif threshold == 'max':
        min_norm_2 = np.square(np.load(fname_templates_in)).sum((1, 2)).min()
        threshold = min_norm_2 * 0.8

    conv_approx_rank = 5
    upsample_max_val = 8
    max_iter = 1000

    if run_chunk_sec == 'full':
        chunk_sec = None
    else:
        chunk_sec = run_chunk_sec

    reader = READER(recordings_filename,
                    recording_dtype,
                    CONFIG,
                    CONFIG.resources.n_sec_chunk,
                    chunk_sec=chunk_sec)

    mp_object = MatchPursuit_objectiveUpsample(
        fname_templates=fname_templates_in,
        save_dir=output_directory,
        reader=reader,
        max_iter=max_iter,
        upsample=upsample_max_val,
        threshold=threshold,
        conv_approx_rank=conv_approx_rank,
        n_processors=CONFIG.resources.n_processors,
        multi_processing=CONFIG.resources.multi_processing)

    logger.info('Number of Units IN: {}'.format(mp_object.temps.shape[2]))

    # directory to save results for each segment
    seg_dir = os.path.join(output_directory, 'seg')
    if not os.path.exists(seg_dir):
        os.makedirs(seg_dir)

    # skip files/batches already completed; this allows more even distribution
    # across cores in case of restart
    # Cat: TODO: if cpu is still being used by endusers, may wish to implement
    #       dynamic file assignment here to deal with slow cores etc.
    fnames_out = []
    batch_ids = []
    for batch_id in range(reader.n_batches):
        fname_temp = os.path.join(
            seg_dir, "seg_{}_deconv.npz".format(str(batch_id).zfill(6)))
        if os.path.exists(fname_temp):
            continue
        fnames_out.append(fname_temp)
        batch_ids.append(batch_id)
    logger.info("running deconvolution on {} batches of {} seconds".format(
        len(batch_ids), CONFIG.resources.n_sec_chunk))

    if len(batch_ids) > 0:
        if CONFIG.resources.multi_processing:
            logger.info("running deconvolution with {} processors".format(
                CONFIG.resources.n_processors))
            batches_in = np.array_split(batch_ids,
                                        CONFIG.resources.n_processors)
            fnames_in = np.array_split(fnames_out,
                                       CONFIG.resources.n_processors)
            parmap.starmap(mp_object.run,
                           list(zip(batches_in, fnames_in)),
                           processes=CONFIG.resources.n_processors,
                           pm_pbar=True)
        else:
            logger.info("running deconvolution")
            for ctr in range(len(batch_ids)):
                mp_object.run([batch_ids[ctr]], [fnames_out[ctr]])

    # collect result
    res = []
    logger.info("gathering deconvolution results")
    for batch_id in range(reader.n_batches):
        fname_out = os.path.join(
            seg_dir, "seg_{}_deconv.npz".format(str(batch_id).zfill(6)))
        res.append(np.load(fname_out)['spike_train'])
    res = np.vstack(res)

    logger.info('Number of Spikes deconvolved: {}'.format(res.shape[0]))

    # save templates and upsampled templates
    np.save(fname_templates, np.load(fname_templates_in))
    #np.save(fname_templates,
    #        mp_object.temps.transpose(2,0,1))

    # since deconv spike time is not centered, get shift for centering
    shift = CONFIG.spike_size // 2

    # get spike train and save
    spike_train = np.copy(res)
    # map back to original id
    spike_train[:,
                1] = np.int32(spike_train[:, 1] / mp_object.upsample_max_val)
    spike_train[:, 0] += shift
    # save
    np.save(fname_spike_train, spike_train)

    if save_up_data:
        # get upsampled templates and mapping for computing residual
        (templates_up, deconv_id_sparse_temp_map
         ) = mp_object.get_sparse_upsampled_templates()

        np.save(fname_templates_up, templates_up.transpose(2, 0, 1))

        # get upsampled spike train
        spike_train_up = np.copy(res)
        spike_train_up[:, 1] = deconv_id_sparse_temp_map[spike_train_up[:, 1]]
        spike_train_up[:, 0] += shift
        np.save(fname_spike_train_up, spike_train_up)
Exemplo n.º 15
0
def run(output_directory,
        fname_recording,
        recording_dtype,
        fname_residual=None,
        residual_dtype=None,
        fname_spike_index=None,
        fname_templates=None,
        fname_spike_train=None,
        fname_shifts=None,
        fname_scales=None,
        raw_data=True,
        full_run=False):
    """Spike clustering

    Parameters
    ----------

    spike_index: numpy.ndarray (n_clear_spikes, 2), str or Path
        2D array with indexes for spikes, first column contains the
        spike location in the recording and the second the main channel
        (channel whose amplitude is maximum). Or path to an npy file

    out_dir: str, optional
        Location to store/look for the generate spike train relative to
        config output directory

    if_file_exists: str, optional
      One of 'overwrite', 'abort', 'skip'. Control de behavior for the
      spike_train_cluster.npy. file If 'overwrite' it replaces the files if
      exists, if 'abort' it raises a ValueError exception if exists,
      if 'skip' it skips the operation if the file exists (and returns the
      stored file)

    save_results: bool, optional
        Whether to save spike train to disk
        (in CONFIG.data.root_folder/relative_to/spike_train_cluster.npy),
        defaults to False

    Returns
    -------
    spike_train: (TODO add documentation)

    Examples
    --------

    .. literalinclude:: ../../examples/pipeline/cluster.py

    """
    logger = logging.getLogger(__name__)

    ########################
    ### INITIALIZE #########
    ########################

    CONFIG = read_config()
    # get CONFIG2 for clustering
    # Cat: TODO: Edu said the CONFIG file can be passed as a dictionary
    CONFIG2 = make_CONFIG2(CONFIG)

    os.environ["CUDA_VISIBLE_DEVICES"] = str(CONFIG.resources.gpu_id)

    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    # data reader
    reader_raw = READER(fname_recording,
                        recording_dtype,
                        CONFIG,
                        CONFIG.resources.n_sec_chunk_gpu_deconv,
                        chunk_sec=CONFIG.clustering_chunk)
    if fname_residual is not None:
        reader_resid = READER(fname_residual,
                              residual_dtype,
                              CONFIG,
                              CONFIG.resources.n_sec_chunk_gpu_deconv,
                              chunk_sec=CONFIG.clustering_chunk)
    else:
        reader_resid = None

    # nn denoiser
    if CONFIG.neuralnetwork.apply_nn:
        # load NN denoiser
        denoiser = Denoise(CONFIG.neuralnetwork.denoise.n_filters,
                           CONFIG.neuralnetwork.denoise.filter_sizes,
                           CONFIG.spike_size_nn, CONFIG)
        denoiser.load(CONFIG.neuralnetwork.denoise.filename)
        denoiser = denoiser.cuda()
    else:
        denoiser = None

    # if the output exists and want to skip, just finish
    fname_templates_out = os.path.join(output_directory, 'templates.npy')
    fname_spike_train_out = os.path.join(output_directory, 'spike_train.npy')
    if not os.path.exists(fname_templates_out):

        # if clustering on clean waveforms, spike train is given
        # => make spike index and labels
        if fname_spike_index is None:
            savedir = os.path.join(output_directory, 'spike_index')
            if not os.path.exists(savedir):
                os.makedirs(savedir)
            (fname_spike_index,
             fname_labels_input) = make_spike_index_from_spike_train(
                 fname_spike_train, fname_templates, savedir)

        else:
            # if we have spike_index, then we have no initial labels
            fname_labels_input = None

        #################################
        #### STAGE 1: Cluster on PTP ####
        #################################

        # keep track of input label because this is the deconv label
        # and it is necessary when making cleaned spikes
        logger.info("Split on PTP")
        (fname_spike_index, fname_labels,
         fname_labels_input) = run_split_on_ptp(
             os.path.join(output_directory, 'ptp_split'), fname_spike_index,
             CONFIG2, raw_data, fname_labels_input, fname_templates,
             fname_shifts, fname_scales, reader_raw, reader_resid, denoiser)

        ############################################
        #### STAGE 2: LOCAL + DISTANT CLUSTERING ###
        ############################################

        # load and align waveforms
        logger.info("load waveforms on local channels")
        units, fnames_input = load_waveforms(
            os.path.join(output_directory, 'input'), raw_data, fname_labels,
            fname_spike_index, fname_labels_input, fname_templates,
            fname_shifts, fname_scales, reader_raw, reader_resid, CONFIG2)

        if CONFIG.neuralnetwork.apply_nn:
            logger.info("NN denoise")
            # denoise it
            nn_denoise_wf(fnames_input, denoiser, CONFIG.torch_devices, CONFIG)
        else:
            logger.info("denoise")
            denoise_wf(fnames_input)

        #if raw_data:
        # align if raw data
        # no need to align for clean waveforms
        # because input shift is already used for alignment
        logger.info("align waveforms on local channels")
        align_waveforms(fnames_input, CONFIG2)

        # save location for intermediate results
        tmp_save_dir = os.path.join(output_directory, 'cluster_result')
        if not os.path.exists(tmp_save_dir):
            os.makedirs(tmp_save_dir)

        # Cat: TODO: this parallelization may not be optimally asynchronous
        # make arg list first
        args_in = []
        for ctr, unit in enumerate(units):

            # check to see if chunk + channel already completed
            filename_postclustering = os.path.join(
                tmp_save_dir, "cluster_result_{}.npz".format(unit))

            # skip
            if os.path.exists(filename_postclustering):
                continue
            args_in.append([
                raw_data, full_run, CONFIG2, reader_raw, reader_resid,
                filename_postclustering, fnames_input[ctr]
            ])

        logger.info("starting clustering")
        if CONFIG.resources.multi_processing:
            parmap.map(Cluster,
                       args_in,
                       processes=CONFIG.resources.n_processors,
                       pm_pbar=True)

        else:
            with tqdm(total=len(args_in)) as pbar:
                for arg_in in args_in:
                    Cluster(arg_in)
                    pbar.update()

        # first gather clustering result
        fname_templates_out, fname_spike_train_out = gather_clustering_result(
            tmp_save_dir, output_directory)

        for fname in fnames_input:
            os.remove(fname)

    #check_long_temp = os.path.join(output_directory, 'long_template.npy')
    #if not os.path.exists(check_long_temp):
    #    logger.info("get longer templates")
    #    fname_templates_out = run_template_computation(
    #        output_directory,
    #        fname_spike_train_out,
    #        reader_raw,
    #        spike_size=CONFIG.spike_size,
    #        multi_processing=CONFIG.resources.multi_processing,
    #        n_processors=CONFIG.resources.n_processors)
    #    np.save(check_long_temp, None)

    #check_low_fr_temp = os.path.join(output_directory, 'check_low_fr_template.npy')
    #if not os.path.exists(check_low_fr_temp):
    #    if CONFIG.neuralnetwork.apply_nn:
    #        # denoise wfs before computing templates for low fr units
    #        logger.info("re-estimate templates of low firing rate units")
    #        fname_templates_out = denoise_then_estimate_template(
    #            fname_templates_out,
    #            fname_spike_train_out,
    #            reader_raw,
    #            denoiser,
    #            CONFIG,
    #            n_max_spikes=100)

    #    np.save(check_low_fr_temp, None)

    #check_sharpen = os.path.join(output_directory, 'check_sharpen.npy')
    #if not os.path.exists(check_sharpen):
    #fname_templates_aligned = os.path.join(output_directory, 'templates_aligned.npy')
    #if not os.path.exists(fname_templates_aligned):
    #    logger.info("subsample template alignment")
    #    fname_templates_out = sharpen_templates(fname_templates_out,
    #                                            fname_templates_aligned)

    # zero-out edges
    #check_zero_out = os.path.join(output_directory, 'check_zero_out.npy')
    #if not os.path.exists(check_zero_out):
    #    logger.info("zero out unnecessary parts")
    #    fix_template_edges_by_file(fname_templates_out,
    #                               CONFIG.center_spike_size)
    #    np.save(check_zero_out, None)

    return fname_templates_out, fname_spike_train_out
Exemplo n.º 16
0
def run(fname_templates_in,
        output_directory,
        recordings_filename,
        recording_dtype,
        threshold=None,
        run_chunk_sec='full',
        save_up_data=True):
            
    """Deconvolute spikes

    Parameters
    ----------

    spike_index_all: numpy.ndarray (n_data, 3)
        A 2D array for all potential spikes whose first column indicates the
        spike time and the second column the principal channels
        3rd column indicates % confidence of cluster membership
        Note: can now have single events assigned to multiple templates

    templates: numpy.ndarray (n_channels, waveform_size, n_templates)
        A 3D array with the templates

    output_directory: str, optional
        Output directory (relative to CONFIG.data.root_folder) used to load
        the recordings to generate templates, defaults to tmp/

    recordings_filename: str, optional
        Recordings filename (relative to CONFIG.data.root_folder/
        output_directory) used to draw the waveforms from, defaults to
        standardized.bin

    Returns
    -------
    spike_train: numpy.ndarray (n_clear_spikes, 2)
        A 2D array with the spike train, first column indicates the spike
        time and the second column the neuron ID

    Examples
    --------

    .. literalinclude:: ../../examples/pipeline/deconvolute.py
    """

    logger = logging.getLogger(__name__)

    CONFIG = read_config()
    CONFIG = make_CONFIG2(CONFIG)

    #print("... deconv using GPU device: ", torch.cuda.current_device())
    
    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    fname_templates = os.path.join(
        output_directory, 'templates.npy')
    fname_spike_train = os.path.join(
        output_directory, 'spike_train.npy')
    fname_shifts = os.path.join(
        output_directory, 'shifts.npy')
    fname_scales = os.path.join(
        output_directory, 'scales.npy')

    if (os.path.exists(fname_templates) and
        os.path.exists(fname_spike_train) and
        os.path.exists(fname_shifts) and
        os.path.exists(fname_scales)):
        return (fname_templates, fname_spike_train,
                fname_shifts, fname_scales)
    # parameters
    if threshold is None:
        threshold = CONFIG.deconvolution.threshold
    elif threshold == 'low_fp':
        threshold = 150

    if run_chunk_sec == 'full':
        chunk_sec = None
    else:
        chunk_sec = run_chunk_sec

    # reader
    reader = READER(recordings_filename,
                    recording_dtype,
                    CONFIG,
                    CONFIG.resources.n_sec_chunk_gpu_deconv,
                    chunk_sec=chunk_sec)
    # enforce broad buffer
    reader.buffer=1000
         
    deconv_ONgpu(fname_templates_in,
                 output_directory,
                 reader,
                 threshold,
                 CONFIG,
                 run_chunk_sec)

    return (fname_templates, fname_spike_train,
            fname_shifts, fname_scales)
Exemplo n.º 17
0
def run(output_directory, fname_spike_train, fname_shifts, fname_scales,
        fname_templates, fname_soft_assignment, fname_residual,
        residual_dtype):

    logger = logging.getLogger(__name__)

    CONFIG = read_config()

    # output folder
    if not os.path.exists(output_directory):
        os.makedirs(output_directory)

    fname_spike_train_out = os.path.join(output_directory, 'spike_train.npy')
    fname_templates_out = os.path.join(output_directory, 'templates.npy')
    fname_soft_assignment_out = os.path.join(output_directory,
                                             'soft_assignment.npy')
    fname_shifts_out = os.path.join(output_directory, 'shifts.npy')
    fname_scales_out = os.path.join(output_directory, 'scales.npy')
    if os.path.exists(fname_spike_train_out) and os.path.exists(
            fname_templates_out):
        return (fname_templates_out, fname_spike_train_out, fname_shifts_out,
                fname_scales_out, fname_soft_assignment_out)

    reader_residual = READER(fname_residual, residual_dtype, CONFIG)

    # get whitening filters
    fname_spatial_cov = os.path.join(output_directory, 'spatial_cov.npy')
    fname_temporal_cov = os.path.join(output_directory, 'temporal_cov.npy')
    if not (os.path.exists(fname_spatial_cov)
            and os.path.exists(fname_temporal_cov)):
        spatial_cov, temporal_cov = get_noise_covariance(
            reader_residual, CONFIG)
        np.save(fname_spatial_cov, spatial_cov)
        np.save(fname_temporal_cov, temporal_cov)
    else:
        spatial_cov = np.load(fname_spatial_cov)
        temporal_cov = np.load(fname_temporal_cov)

    # initialize merge: find candidates
    logger.info("finding merge candidates")
    tm = TemplateMerge(output_directory, reader_residual, fname_templates,
                       fname_spike_train, fname_shifts, fname_scales,
                       fname_soft_assignment, fname_spatial_cov,
                       fname_temporal_cov, CONFIG.geom,
                       CONFIG.resources.multi_processing,
                       CONFIG.resources.n_processors)

    # find merge pairs
    logger.info("merging pairs")
    tm.get_merge_pairs()

    # update templates adn spike train accordingly
    logger.info("udpating templates and spike train")
    (templates_new, spike_train_new, shifts_new, scales_new,
     soft_assignment_new, merge_array) = tm.merge_units()

    # save results
    fname_merge_array = os.path.join(output_directory, 'merge_array.npy')
    np.save(fname_merge_array, merge_array)
    np.save(fname_spike_train_out, spike_train_new)
    np.save(fname_templates_out, templates_new)
    np.save(fname_shifts_out, shifts_new)
    np.save(fname_scales_out, scales_new)
    np.save(fname_soft_assignment_out, soft_assignment_new)

    logger.info('Number of units after merge: {}'.format(
        templates_new.shape[0]))

    return (fname_templates_out, fname_spike_train_out, fname_shifts_out,
            fname_scales_out, fname_soft_assignment_out)
Exemplo n.º 18
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def run_cleaned_template_computation(out_dir,
                                     fname_spike_train,
                                     fname_templates,
                                     fname_shifts,
                                     fname_scales,
                                     fname_residual_recording,
                                     dtype_residual_recording,
                                     CONFIG,
                                     unit_ids=None):

    logger = logging.getLogger(__name__)

    logger.info("computing templates from cleaned spikes")

    # make output folder
    if not os.path.exists(out_dir):
        os.makedirs(out_dir)

    #fname_templates = os.path.join(out_dir, 'templates.npy')
    #if os.path.exists(fname_templates):
    #    return fname_templates

    # make temp folder
    tmp_folder = os.path.join(out_dir, 'tmp_template')
    if not os.path.exists(tmp_folder):
        os.makedirs(tmp_folder)

    # get number of units
    n_units, n_times, n_channels = np.load(fname_templates).shape
    if unit_ids is None:
        unit_ids = np.arange(n_units)

    reader_residual = READER(fname_residual_recording,
                             dtype_residual_recording, CONFIG)

    # run computing function
    if CONFIG.resources.multi_processing:
        n_processors = CONFIG.resources.n_processors
        unit_ids_partition = []
        for j in range(n_processors):
            unit_ids_partition.append(unit_ids[slice(j, len(unit_ids),
                                                     n_processors)])

        parmap.map(run_cleaned_template_computation_parallel,
                   unit_ids_partition,
                   tmp_folder,
                   fname_spike_train,
                   fname_templates,
                   fname_shifts,
                   fname_scales,
                   reader_residual,
                   pm_processes=n_processors,
                   pm_pbar=True)

    else:
        run_cleaned_template_computation_parallel(unit_ids, tmp_folder,
                                                  fname_spike_train,
                                                  fname_templates,
                                                  fname_shifts, fname_scales,
                                                  reader_residual)

    # gather all info
    templates_new = np.zeros((n_units, n_times, n_channels), 'float32')
    for unit in unit_ids:
        fname_out = os.path.join(tmp_folder, 'unit_{}.npy'.format(unit))
        templates_new[unit] = np.load(fname_out)

    fname_templates = os.path.join(out_dir, 'templates.npy')
    np.save(fname_templates, templates_new)

    return fname_templates