def generatePeakSets(infile, outfiles):
outf_con, outf_opt = outfiles
# retrieve maximum number of peaks obtained from inter-replicate IDR
statement = ("SELECT"
" experiment,"
" max(n_peaks) AS nPeaks"
" FROM individual_replicates_nPeaks"
" GROUP BY experiment")
df = PU.fetch_DataFrame(statement)
# reassign experiment as index
df = df.set_index("experiment")
# retrieve number of peaks obtained from pooled_pseudoreplicate IDR
statement = ("SELECT"
" experiment,"
" n_peaks AS nPeaks"
" FROM pooled_pseudoreplicates_nPeaks")
df2 = PU.fetch_DataFrame(statement)
# reassign experiment as index
df2 = df2.set_index("experiment")
# split the infile name to obtain experiment
sample_id = os.path.basename(infile).split("_VS_")[0]
sample = sample_id.split("-")
experiment = "_".join([sample[0], sample[1]])
# retrieve max_numPeaks for experiment
nPeaks = int(df.loc[experiment])
# retrieve numPeaks_Rep0 for experiment
nPeaks_rep0 = int(df2.loc[experiment])
# retrieve maximumn of the two
nPeaks_max = max(nPeaks, nPeaks_rep0)
# establish which column to sort by
if PARAMS["idr_options_ranking_measure"] == "signal.value":
sort_statement = "sort -k7nr,7nr"
elif PARAMS["idr_options_ranking_measure"] == "p.value":
sort_statement = "sort -k8nr,8nr"
elif PARAMS["idr_options_ranking_measure"] == "q.value":
sort_statement = "sort -k9nr,9nr"
else:
raise ValueError("Unrecognised ranking_measure"
" %s don't know which column"
" to sort on" % PARAMS["idr_options_ranking_measure"])
# sort infile by column and write top nPeaks to outfile (conservative)
ignore_pipe_errors = True
statement = ("zcat %(infile)s |"
" %(sort_statement)s |"
" head -%(nPeaks)s |"
" gzip > %(outf_con)s")
P.run()
# sort infile by column and write top nPeaks_max to outfile (optimum)
ignore_pipe_errors = True
statement = ("zcat %(infile)s |"
" %(sort_statement)s |"
" head -%(nPeaks)s |"
" gzip > %(outf_opt)s")
P.run()
def interactions2BedGraph(track,
probe,
db,
outfile,
chrom=None,
start=None,
end=None,
region_size=1000000,
window=2000,
step=200):
import CGATPipelines.PipelineUtilities as PUtils
import pandas
import numpy as np
E.debug("Get Probe fragment")
#get probe fragment
statement = '''SELECT chr, start, end, fragment
FROM probe_fragments
INNER JOIN probe_fragments_lookup as lu
ON probe_fragments.name = lu.probe
WHERE name = '%(probe)s' '''
probes = PUtils.fetch_DataFrame(statement % locals(), database=db)
probe_frag = probes.fragment.iloc[0]
if chrom is None:
probe_chr = probes.chr.iloc[0]
probe_centre = 0.5 * (probes.start.iloc[0] + probes.end.iloc[0])
if start is None:
start = probe_centre - region_size / 2
if end is None:
end = probe_centre + region_size / 2
E.debug("Fetch data")
statement = '''SELECT (start+end)/2 as centre,
count
FROM interaction_counts as ic
INNER JOIN fragments
ON fragments.name = ic.Frag2
WHERE abs(%(probe_frag)s - ic.Frag2) > 1
AND Frag1 = '%(probe_frag)s' AND track = '%(track)s'
AND chr='%(probe_chr)s'
AND centre > %(start)s AND centre <= %(end)s
AND NOT ABS(centre - %(probe_start)) < 2000
ORDER BY centre '''
E.debug(statement % locals())
interactions = PUtils.fetch_DataFrame(statement % locals(), db)
E.debug("Got %i interacting fragments" % interactions.shape[0])
interactions["centre"] = interactions["centre"].astype("float64")
interactions.set_index("centre", inplace=True)
interactions = interactions.sort_index()
window_starts = pandas.Series(np.arange(start, end, step))
window_starts.index = window_starts.values
window_starts.index.name = "start"
def _applyToWindow(val):
#E.debug(val)
return interactions.loc[(val -
window / 2):(val +
window / 2)]["Count"].sum()
E.debug("rolling")
windowed = window_starts.apply(_applyToWindow)
assert windowed.sum() >= interactions["Count"].sum(), \
"windowed sum %s, total count %s" % (windowed.sum(), interactions["Count"].sum())
E.debug("format output")
windowed = windowed.reset_index()
windowed["end"] = windowed["start"] + step
windowed["chr"] = probe_chr
windowed = windowed[["chr", "start", "end", 0]]
windowed.to_csv(IOTools.openFile(outfile, "w"),
sep="\t",
index=False,
header=False)
def generatePeakSets(infile, outfiles):
outf_con, outf_opt = outfiles
# retrieve maximum number of peaks obtained from inter-replicate IDR
statement = ("SELECT"
" experiment,"
" max(n_peaks) AS nPeaks"
" FROM individual_replicates_nPeaks"
" GROUP BY experiment")
df = PU.fetch_DataFrame(statement)
# reassign experiment as index
df = df.set_index("experiment")
# retrieve number of peaks obtained from pooled_pseudoreplicate IDR
statement = ("SELECT"
" experiment,"
" n_peaks AS nPeaks"
" FROM pooled_pseudoreplicates_nPeaks")
df2 = PU.fetch_DataFrame(statement)
# reassign experiment as index
df2 = df2.set_index("experiment")
# split the infile name to obtain experiment
sample_id = os.path.basename(infile).split("_VS_")[0]
sample = sample_id.split("-")
experiment = "_".join([sample[0], sample[1]])
# retrieve max_numPeaks for experiment
nPeaks = int(df.loc[experiment])
# retrieve numPeaks_Rep0 for experiment
nPeaks_rep0 = int(df2.loc[experiment])
# retrieve maximumn of the two
nPeaks_max = max(nPeaks, nPeaks_rep0)
# establish which column to sort by
if PARAMS["idr_options_ranking_measure"] == "signal.value":
sort_statement = "sort -k7nr,7nr"
elif PARAMS["idr_options_ranking_measure"] == "p.value":
sort_statement = "sort -k8nr,8nr"
elif PARAMS["idr_options_ranking_measure"] == "q.value":
sort_statement = "sort -k9nr,9nr"
else:
raise ValueError("Unrecognised ranking_measure"
" %s don't know which column"
" to sort on" % PARAMS["idr_options_ranking_measure"])
# sort infile by column and write top nPeaks to outfile (conservative)
ignore_pipe_errors = True
statement = ("zcat %(infile)s |"
" %(sort_statement)s |"
" head -%(nPeaks)s |"
" gzip > %(outf_con)s")
P.run()
# sort infile by column and write top nPeaks_max to outfile (optimum)
ignore_pipe_errors = True
statement = ("zcat %(infile)s |"
" %(sort_statement)s |"
" head -%(nPeaks)s |"
" gzip > %(outf_opt)s")
P.run()
def interactions2BedGraph(track, probe, db, outfile, chrom=None, start=None, end=None,
region_size=1000000, window=2000, step=200):
import CGATPipelines.PipelineUtilities as PUtils
import pandas
import numpy as np
E.debug("Get Probe fragment")
#get probe fragment
statement = '''SELECT chr, start, end, fragment
FROM probe_fragments
INNER JOIN probe_fragments_lookup as lu
ON probe_fragments.name = lu.probe
WHERE name = '%(probe)s' '''
probes = PUtils.fetch_DataFrame(statement % locals(), database=db)
probe_frag = probes.fragment.iloc[0]
if chrom is None:
probe_chr = probes.chr.iloc[0]
probe_centre = 0.5*(probes.start.iloc[0] + probes.end.iloc[0])
if start is None:
start = probe_centre - region_size/2
if end is None:
end = probe_centre + region_size/2
E.debug("Fetch data")
statement = '''SELECT (start+end)/2 as centre,
count
FROM interaction_counts as ic
INNER JOIN fragments
ON fragments.name = ic.Frag2
WHERE abs(%(probe_frag)s - ic.Frag2) > 1
AND Frag1 = '%(probe_frag)s' AND track = '%(track)s'
AND chr='%(probe_chr)s'
AND centre > %(start)s AND centre <= %(end)s
AND NOT ABS(centre - %(probe_start)) < 2000
ORDER BY centre '''
E.debug(statement % locals())
interactions = PUtils.fetch_DataFrame(statement % locals(), db)
E.debug("Got %i interacting fragments" % interactions.shape[0])
interactions["centre"] = interactions["centre"].astype("float64")
interactions.set_index("centre", inplace=True)
interactions = interactions.sort_index()
window_starts = pandas.Series(np.arange(start, end, step))
window_starts.index = window_starts.values
window_starts.index.name = "start"
def _applyToWindow(val):
#E.debug(val)
return interactions.loc[(val-window/2):(val+window/2)]["Count"].sum()
E.debug("rolling")
windowed = window_starts.apply(_applyToWindow)
assert windowed.sum() >= interactions["Count"].sum(), \
"windowed sum %s, total count %s" % (windowed.sum(), interactions["Count"].sum())
E.debug("format output")
windowed = windowed.reset_index()
windowed["end"] = windowed["start"] + step
windowed["chr"] = probe_chr
windowed = windowed[["chr", "start", "end", 0]]
windowed.to_csv(IOTools.openFile(outfile, "w"), sep = "\t",
index=False, header=False)
