def main(args):
star = parse_star(args.input, keep_index=False)
if args.class_2d is not None:
refs = glob.glob(args.class_2d)
elif args.class_3d is not None:
refs = glob.glob(args.class_3d)
else:
refs = []
shifts = []
for r in refs:
if args.class_3d is not None:
refmap = EMData(r)
com = Vec3f(*refmap.phase_cog()[:3])
shifts.append(com)
else:
stack = EMData.read_images(r)
for im in stack:
com = Vec2f(*im.phase_cog()[:2])
shifts.append(com)
if args.class_2d is None and args.class_3d is None:
for ptcl in star.rows:
im = EMData.read_image(ptcl)
com = im.phase_cog()
ptcl["rlnOriginX"] += com[0]
ptcl["rlnOriginY"] += com[1]
else:
for ptcl in star.rows:
com = shifts[ptcl["rlnClassNumber"]]
xshift, yshift = transform_com(com, ptcl)
ptcl["rlnOriginX"] += xshift
ptcl["rlnOriginY"] += yshift
if args.zero_origin:
star["rlnCoordinateX"] = star["rlnCoordinateX"] - star["rlnOriginX"]
star["rlnCoordinateY"] = star["rlnCoordinateY"] - star["rlnOriginY"]
star["rlnOriginX"] = 0
star["rlnOriginY"] = 0
write_star(args.output, star, reindex=True)
return 0
def main(options):
"""
Projection subtraction program entry point.
:param options: Command-line arguments parsed by ArgumentParser.parse_args()
:return: Exit status
"""
rchop = lambda x, y: x if not x.endswith(y) or len(y) == 0 else x[:-len(y)]
options.output = rchop(options.output, ".star")
options.suffix = rchop(options.suffix, ".mrc")
options.suffix = rchop(options.suffix, ".mrcs")
star = StarFile(options.input)
npart = len(star['rlnImageName'])
sub_dens = EMData(options.submap)
if options.wholemap is not None:
dens = EMData(options.wholemap)
else:
print "Reference map is required."
return 1
# Write star header for output.star.
top_header = "\ndata_\n\nloop_\n"
headings = star.keys()
output_star = open("{0}.star".format(options.output), 'w')
output_star.write(top_header)
for i, heading in enumerate(headings):
output_star.write("_{0} #{1}\n".format(heading, i + 1))
if options.recenter: # Compute difference vector between new and old mass centers.
if options.wholemap is None:
print "Reference map required for recentering."
return 1
new_dens = dens - sub_dens
# Note the sign of the shift in coordinate frame is opposite the shift in the CoM.
recenter = Vec3f(*dens.phase_cog()[:3]) - Vec3f(
*new_dens.phase_cog()[:3])
else:
recenter = None
pool = None
if options.nproc > 1: # Compute subtraction in parallel.
pool = Pool(processes=options.nproc)
results = pool.imap(
lambda x: subtract(x,
dens,
sub_dens,
recenter=recenter,
no_frc=options.no_frc,
low_cutoff=options.low_cutoff,
high_cutoff=options.high_cutoff),
particles(star),
chunksize=min(npart / options.nproc, options.maxchunk))
else: # Use serial generator.
results = (subtract(x,
dens,
sub_dens,
recenter=recenter,
no_frc=options.no_frc,
low_cutoff=options.low_cutoff,
high_cutoff=options.high_cutoff)
for x in particles(star))
# Write subtraction results to .mrcs and .star files.
i = 0
nfile = 1
starpath = None
mrcs = None
mrcs_orig = None
for r in results:
if i % options.maxpart == 0:
mrcsuffix = options.suffix + "_%d" % nfile
nfile += 1
starpath = "{0}.mrcs".format(
os.path.sep.join(
os.path.relpath(mrcsuffix,
options.output).split(os.path.sep)[1:]))
mrcs = "{0}.mrcs".format(mrcsuffix)
mrcs_orig = "{0}_original.mrcs".format(mrcsuffix)
if os.path.exists(mrcs):
os.remove(mrcs)
if os.path.exists(mrcs_orig):
os.remove(mrcs_orig)
r.ptcl_norm_sub.append_image(mrcs)
if options.original:
r.ptcl.append_image(mrcs_orig)
if logger.getEffectiveLevel(
) == logging.DEBUG: # Write additional debug output.
ptcl_sub_img = r.ptcl.process("math.sub.optimal", {
"ref": r.ctfproj,
"actual": r.ctfproj_sub,
"return_subim": True
})
ptcl_lowpass = r.ptcl.process("filter.lowpass.gauss", {
"apix": 1.22,
"cutoff_freq": 0.05
})
ptcl_sub_lowpass = r.ptcl_norm_sub.process("filter.lowpass.gauss",
{
"apix": 1.22,
"cutoff_freq": 0.05
})
ptcl_sub_img.write_image("poreclass_subimg.mrcs", -1)
ptcl_lowpass.write_image("poreclass_lowpass.mrcs", -1)
ptcl_sub_lowpass.write_image("poreclass_sublowpass.mrcs", -1)
r.ctfproj.write_image("poreclass_ctfproj.mrcs", -1)
r.ctfproj_sub.write_image("poreclass_ctfprojsub.mrcs", -1)
assert r.meta.i == i # Assert particle order is preserved.
star['rlnImageName'][i] = "{0:06d}@{1}".format(
i % options.maxpart + 1, starpath) # Set new image name.
r.meta.update(star) # Update StarFile with altered fields.
line = ' '.join(str(star[key][i]) for key in headings)
output_star.write("{0}\n".format(line))
i += 1
output_star.close()
if pool is not None:
pool.close()
pool.join()
return 0
