def __init__(self, trp, vol=15, maxdil=16, mindilvol=90, maxdilvol=100, debug=False): 'Create a new QPCR setup structure' self.volume = vol self.samples = [] self.needDil = [] self.primers = [] self.nreplicates = [] self.dilProds = [] self.reuse = [ ] # Index of prior dilution that can be used as input to this one; otherwise None self.stages = [] self.MAXDIL = maxdil self.MINDILVOL = mindilvol self.MAXDILVOL = maxdilvol self.trp = trp self.debug = debug self.dilutant = decklayout.SSDDIL self.jobq = JobQueue() self.e = Experiment()