Пример #1
0
    def __init__(self, hits=[], id=None,
            hit_key_function=lambda hit: hit.id):
        """Initializes a QueryResult object.

        Arguments:
        id -- String of query sequence ID.
        hits -- Iterator returning Hit objects.
        hit_key_function -- Function to define hit keys, defaults to a function
                            that return Hit object IDs.

        """
        # default values
        self._id = id
        self._hit_key_function = hit_key_function
        self._items = OrderedDict()
        self._description = None
        self.program = '<unknown program>'
        self.target = '<unknown target>'
        self.version = '<unknown version>'

        # validate Hit objects and fill up self._items
        for hit in hits:
            # validation is handled by __setitem__
            self.append(hit)
Пример #2
0
    def sort(self, key=None, reverse=False, in_place=True):
        # no cmp argument to make sort more Python 3-like
        """Sorts the Hit objects.

        Arguments:
        key -- Function used to sort the Hit objects.
        reverse -- Boolean, whether to reverse the sorting or not.
        in_place -- Boolean, whether to perform sorting in place (in the same
                    object) or not (creating a new object).

        `sort` defaults to sorting in-place, to mimick Python's `list.sort`
        method. If you set the `in_place` argument to False, it will treat
        return a new, sorted QueryResult object and keep the initial one
        unsorted.

        """
        if key is None:
            # if reverse is True, reverse the hits
            if reverse:
                sorted_hits = list(self.hits)[::-1]
            # otherwise (default options) make a copy of the hits
            else:
                sorted_hits = list(self.hits)[:]
        else:
            sorted_hits = sorted(self.hits, key=key, reverse=reverse)

        # if sorting is in-place, don't create a new QueryResult object
        if in_place:
            new_hits = OrderedDict()
            for hit in sorted_hits:
                new_hits[self._hit_key_function(hit)] = hit
            self._items = new_hits
        # otherwise, return a new sorted QueryResult object
        else:
            obj = self.__class__(sorted_hits, self.id, self._hit_key_function)
            self._transfer_attrs(obj)
            return obj
Пример #3
0
class QueryResult(_BaseSearchObject):

    """Class representing search results from a single query.

    QueryResult is the container object that stores all search hits from a
    single search query. It is the top-level object returned by SearchIO's two
    main functions, `read` and `parse`. Depending on the search results and
    search output format, a QueryResult object will contain zero or more Hit
    objects (see Hit).

    You can take a quick look at a QueryResult's contents and attributes by
    invoking `print` on it:

    >>> from SAP.Bio import SearchIO
    >>> qresult = next(SearchIO.parse('Blast/mirna.xml', 'blast-xml'))
    >>> print(qresult)
    Program: blastn (2.2.27+)
      Query: 33211 (61)
             mir_1
     Target: refseq_rna
       Hits: ----  -----  ----------------------------------------------------------
                #  # HSP  ID + description                                          
             ----  -----  ----------------------------------------------------------
                0      1  gi|262205317|ref|NR_030195.1|  H**o sapiens microRNA 52...
                1      1  gi|301171311|ref|NR_035856.1|  Pan troglodytes microRNA...
                2      1  gi|270133242|ref|NR_032573.1|  Macaca mulatta microRNA ...
                3      2  gi|301171322|ref|NR_035857.1|  Pan troglodytes microRNA...
                4      1  gi|301171267|ref|NR_035851.1|  Pan troglodytes microRNA...
                5      2  gi|262205330|ref|NR_030198.1|  H**o sapiens microRNA 52...
                6      1  gi|262205302|ref|NR_030191.1|  H**o sapiens microRNA 51...
                7      1  gi|301171259|ref|NR_035850.1|  Pan troglodytes microRNA...
                8      1  gi|262205451|ref|NR_030222.1|  H**o sapiens microRNA 51...
                9      2  gi|301171447|ref|NR_035871.1|  Pan troglodytes microRNA...
               10      1  gi|301171276|ref|NR_035852.1|  Pan troglodytes microRNA...
               11      1  gi|262205290|ref|NR_030188.1|  H**o sapiens microRNA 51...
    ...

    If you just want to know how many hits a QueryResult has, you can invoke
    `len` on it. Alternatively, you can simply type its name in the interpreter:

    >>> len(qresult)
    100
    >>> qresult
    QueryResult(id='33211', 100 hits)

    QueryResult behaves like a hybrid of Python's built-in list and dictionary.
    You can retrieve its items (Hit objects) using the integer index of the
    item, just like regular Python lists:

    >>> first_hit = qresult[0]
    >>> first_hit
    Hit(id='gi|262205317|ref|NR_030195.1|', query_id='33211', 1 hsps)

    You can slice QueryResult objects as well. Slicing will return a new
    QueryResult object containing only the sliced hits:

    >>> sliced_qresult = qresult[:3]    # slice the first three hits
    >>> len(qresult)
    100
    >>> len(sliced_qresult)
    3
    >>> print(sliced_qresult)
    Program: blastn (2.2.27+)
      Query: 33211 (61)
             mir_1
     Target: refseq_rna
       Hits: ----  -----  ----------------------------------------------------------
                #  # HSP  ID + description                                          
             ----  -----  ----------------------------------------------------------
                0      1  gi|262205317|ref|NR_030195.1|  H**o sapiens microRNA 52...
                1      1  gi|301171311|ref|NR_035856.1|  Pan troglodytes microRNA...
                2      1  gi|270133242|ref|NR_032573.1|  Macaca mulatta microRNA ...

    Like Python dictionaries, you can also retrieve hits using the hit's ID.
    This is useful for retrieving hits that you know should exist in a given
    search:

    >>> hit = qresult['gi|262205317|ref|NR_030195.1|']
    >>> hit
    Hit(id='gi|262205317|ref|NR_030195.1|', query_id='33211', 1 hsps)

    You can also replace a Hit in QueryResult with another Hit using either the
    integer index or hit key string. Note that the replacing object must be a
    Hit that has the same `query_id` property as the QueryResult object.

    If you're not sure whether a QueryResult contains a particular hit, you can
    use the hit ID to check for membership first:

    >>> 'gi|262205317|ref|NR_030195.1|' in qresult
    True
    >>> 'gi|262380031|ref|NR_023426.1|' in qresult
    False

    Or, if you just want to know the rank / position of a given hit, you can
    use the hit ID as an argument for the `index` method. Note that the values
    returned will be zero-based. So zero (0) means the hit is the first in the
    QueryResult, three (3) means the hit is the fourth item, and so on. If the
    hit does not exist in the QueryResult, a `ValueError` will be raised.

    >>> qresult.index('gi|262205317|ref|NR_030195.1|')
    0
    >>> qresult.index('gi|262205330|ref|NR_030198.1|')
    5
    >>> qresult.index('gi|262380031|ref|NR_023426.1|')
    Traceback (most recent call last):
    ...
    ValueError: ...

    To ease working with a large number of hits, QueryResult has several
    `filter` and `map` methods, analogous to Python's built-in functions with
    the same names. There are `filter` and `map` methods available for
    operations over both Hit objects or HSP objects. As an example, here we are
    using the `hit_map` method to rename all hit IDs within a QueryResult:

    >>> def renamer(hit):
    ...     hit.id = hit.id.split('|')[3]
    ...     return hit
    >>> mapped_qresult = qresult.hit_map(renamer)
    >>> print(mapped_qresult)
    Program: blastn (2.2.27+)
      Query: 33211 (61)
             mir_1
     Target: refseq_rna
       Hits: ----  -----  ----------------------------------------------------------
                #  # HSP  ID + description                                          
             ----  -----  ----------------------------------------------------------
                0      1  NR_030195.1  H**o sapiens microRNA 520b (MIR520B), micr...
                1      1  NR_035856.1  Pan troglodytes microRNA mir-520b (MIR520B...
                2      1  NR_032573.1  Macaca mulatta microRNA mir-519a (MIR519A)...
    ...

    The principle for other `map` and `filter` methods are similar: they accept
    a function, applies it, and returns a new QueryResult object.

    There are also other methods useful for working with list-like objects:
    `append`, `pop`, and `sort`. More details and examples are available in
    their respective documentations.

    Finally, just like Python lists and dictionaries, QueryResult objects are
    iterable. Iteration over QueryResults will yield Hit objects:

    >>> for hit in qresult[:4]:     # iterate over the first four items
    ...     hit
    ...
    Hit(id='gi|262205317|ref|NR_030195.1|', query_id='33211', 1 hsps)
    Hit(id='gi|301171311|ref|NR_035856.1|', query_id='33211', 1 hsps)
    Hit(id='gi|270133242|ref|NR_032573.1|', query_id='33211', 1 hsps)
    Hit(id='gi|301171322|ref|NR_035857.1|', query_id='33211', 2 hsps)

    If you need access to all the hits in a QueryResult object, you can get
    them in a list using the `hits` property. Similarly, access to all hit IDs is
    available through the `hit_keys` property.

    >>> qresult.hits
    [Hit(id='gi|262205317|ref|NR_030195.1|', query_id='33211', 1 hsps), ...]
    >>> qresult.hit_keys
    ['gi|262205317|ref|NR_030195.1|', 'gi|301171311|ref|NR_035856.1|', ...]

    """

    # attributes we don't want to transfer when creating a new QueryResult class
    # from this one
    _NON_STICKY_ATTRS = ('_items',)

    def __init__(self, hits=[], id=None,
            hit_key_function=lambda hit: hit.id):
        """Initializes a QueryResult object.

        Arguments:
        id -- String of query sequence ID.
        hits -- Iterator returning Hit objects.
        hit_key_function -- Function to define hit keys, defaults to a function
                            that return Hit object IDs.

        """
        # default values
        self._id = id
        self._hit_key_function = hit_key_function
        self._items = OrderedDict()
        self._description = None
        self.program = '<unknown program>'
        self.target = '<unknown target>'
        self.version = '<unknown version>'

        # validate Hit objects and fill up self._items
        for hit in hits:
            # validation is handled by __setitem__
            self.append(hit)

    # handle Python 2 OrderedDict behavior
    if hasattr(OrderedDict, 'iteritems'):

        def __iter__(self):
            return self.iterhits()

        @property
        def hits(self):
            """Hit objects contained in the QueryResult."""
            return self._items.values()

        @property
        def hit_keys(self):
            """Hit IDs of the Hit objects contained in the QueryResult."""
            return self._items.keys()

        @property
        def items(self):
            """List of tuples of Hit IDs and Hit objects."""
            return self._items.items()

        def iterhits(self):
            """Returns an iterator over the Hit objects."""
            for hit in self._items.itervalues():
                yield hit

        def iterhit_keys(self):
            """Returns an iterator over the ID of the Hit objects."""
            for hit_id in self._items:
                yield hit_id

        def iteritems(self):
            """Returns an iterator yielding tuples of Hit ID and Hit objects."""
            for item in self._items.iteritems():
                yield item

    else:

        def __iter__(self):
            return iter(self.hits)

        @property
        def hits(self):
            """Hit objects contained in the QueryResult."""
            return list(self._items.values())

        @property
        def hit_keys(self):
            """Hit IDs of the Hit objects contained in the QueryResult."""
            return list(self._items.keys())

        @property
        def items(self):
            """List of tuples of Hit IDs and Hit objects."""
            return list(self._items.items())

        def iterhits(self):
            """Returns an iterator over the Hit objects."""
            for hit in self._items.values():
                yield hit

        def iterhit_keys(self):
            """Returns an iterator over the ID of the Hit objects."""
            for hit_id in self._items:
                yield hit_id

        def iteritems(self):
            """Returns an iterator yielding tuples of Hit ID and Hit objects."""
            for item in self._items.items():
                yield item

    def __contains__(self, hit_key):
        if isinstance(hit_key, Hit):
            return self._hit_key_function(hit_key) in self._items
        return hit_key in self._items

    def __len__(self):
        return len(self._items)

    #Python 3:
    def __bool__(self):
        return bool(self._items)

    #Python 2:
    __nonzero__= __bool__

    def __repr__(self):
        return "QueryResult(id=%r, %r hits)" % (self.id, len(self))

    def __str__(self):
        lines = []

        # set program and version line
        lines.append('Program: %s (%s)' % (self.program, self.version))

        # set query id line
        qid_line = '  Query: %s' % self.id
        if hasattr(self, 'seq_len'):
            qid_line += ' (%i)' % self.seq_len
        if self.description:
            qid_line += trim_str('\n         %s' % self.description, 80, '...')
        lines.append(qid_line)

        # set target line
        lines.append(' Target: %s' % self.target)

        # set hit lines
        if not self.hits:
            lines.append('   Hits: 0')
        else:
            lines.append('   Hits: %s  %s  %s' % ('-'*4, '-'*5, '-'*58))
            pattern = '%13s  %5s  %56s'
            lines.append(pattern % ('#', '# HSP',
                'ID + description'.ljust(58)))
            lines.append(pattern % ('-'*4, '-'*5, '-'*58))
            for idx, hit in enumerate(self.hits):
                if idx < 30:
                    hid_line = '%s  %s' % (hit.id, hit.description)
                    if len(hid_line) > 58:
                        hid_line = hid_line[:55] + '...'
                    lines.append(pattern % (idx, str(len(hit)),
                        hid_line.ljust(58)))
                elif idx > len(self.hits) - 4:
                    hid_line = '%s  %s' % (hit.id, hit.description)
                    if len(hid_line) > 58:
                        hid_line = hid_line[:55] + '...'
                    lines.append(pattern % (idx, str(len(hit)),
                        hid_line.ljust(58)))
                elif idx == 30:
                    lines.append('%14s' % '~~~')

        return '\n'.join(lines)

    def __getitem__(self, hit_key):
        # retrieval using slice objects returns another QueryResult object
        if isinstance(hit_key, slice):
            # should we return just a list of Hits instead of a full blown
            # QueryResult object if it's a slice?
            hits = list(self.hits)[hit_key]
            obj = self.__class__(hits, self.id, self._hit_key_function)
            self._transfer_attrs(obj)
            return obj

        # if key is an int, then retrieve the Hit at the int index
        elif isinstance(hit_key, int):
            length = len(self)
            if 0 <= hit_key < length:
                for idx, item in enumerate(self.iterhits()):
                    if idx == hit_key:
                        return item
            elif -1 * length <= hit_key < 0:
                for idx, item in enumerate(self.iterhits()):
                    if length + hit_key == idx:
                        return item
            raise IndexError("list index out of range")

        # if key is a string, then do a regular dictionary retrieval
        return self._items[hit_key]

    def __setitem__(self, hit_key, hit):
        # only accept string keys
        if not isinstance(hit_key, basestring):
            raise TypeError("QueryResult object keys must be a string.")
        # hit must be a Hit object
        if not isinstance(hit, Hit):
            raise TypeError("QueryResult objects can only contain Hit objects.")
        qid = self.id
        hqid = hit.query_id
        # and it must have the same query ID as this object's ID
        # unless it's the query ID is None (default for empty objects), in which
        # case we want to use the hit's query ID as the query ID
        if qid is not None:
            if hqid != qid:
                raise ValueError("Expected Hit with query ID %r, found %r "
                        "instead." % (qid, hqid))
        else:
            self.id = hqid
        # same thing with descriptions
        qdesc = self.description
        hqdesc = hit.query_description
        if qdesc is not None:
            if hqdesc != qdesc:
                raise ValueError("Expected Hit with query description %r, "
                        "found %r instead." % (qdesc, hqdesc))
        else:
            self.description = hqdesc

        self._items[hit_key] = hit

    def __delitem__(self, hit_key):
        # if hit_key an integer or slice, get the corresponding key first
        # and put it into a list
        if isinstance(hit_key, int):
            hit_keys = [list(self.hit_keys)[hit_key]]
        # the same, if it's a slice
        elif isinstance(hit_key, slice):
            hit_keys = list(self.hit_keys)[hit_key]
        # otherwise put it in a list
        else:
            hit_keys = [hit_key]

        for key in hit_keys:
            del self._items[key]
        return

    ## properties ##
    id = optionalcascade('_id', 'query_id', """QueryResult ID string""")
    description = optionalcascade('_description', 'query_description',
            """QueryResult description""")

    @property
    def hsps(self):
        """HSP objects contained in the QueryResult."""
        return [hsp for hsp in chain(*self.hits)]

    @property
    def fragments(self):
        """HSPFragment objects contained in the QueryResult."""
        return [frag for frag in chain(*self.hsps)]

    ## public methods ##
    def absorb(self, hit):
        """Adds a Hit object to the end of QueryResult. If the QueryResult
        already has a Hit with the same ID, append the new Hit's HSPs into
        the existing Hit.

        Arguments:
        hit -- Hit object to absorb.

        This method is used for file formats that may output the same Hit in
        separate places, such as BLAT or Exonerate. In both formats, Hit
        with different strands are put in different places. However, SearchIO
        considers them to be the same as a Hit object should be all database
        entries with the same ID, regardless of strand orientation.

        """
        try:
            self.append(hit)
        except ValueError:
            assert hit.id in self
            for hsp in hit:
                self[hit.id].append(hsp)

    def append(self, hit):
        """Adds a Hit object to the end of QueryResult.

        Parameters
        hit -- Hit object to append.

        Any Hit object appended must have the same `query_id` property as the
        QueryResult's `id` property. If the hit key already exists, a
        `ValueError` will be raised.

        """
        # if a custom hit_key_function is supplied, use it to define th hit key
        if self._hit_key_function is not None:
            hit_key = self._hit_key_function(hit)
        else:
            hit_key = hit.id

        if hit_key not in self:
            self[hit_key] = hit
        else:
            raise ValueError("Hit '%s' already present in this QueryResult." %
                    hit_key)

    def hit_filter(self, func=None):
        """Creates a new QueryResult object whose Hit objects pass the filter
        function.

        Arguments:
        func -- Callback function that accepts a Hit object as its parameter,
                does a boolean check, and returns True or False

        Here is an example of using `hit_filter` to select Hits whose
        description begins with the string 'H**o sapiens', case sensitive:

        >>> from SAP.Bio import SearchIO
        >>> qresult = next(SearchIO.parse('Blast/mirna.xml', 'blast-xml'))
        >>> def desc_filter(hit):
        ...     return hit.description.startswith('H**o sapiens')
        ...
        >>> len(qresult)
        100
        >>> filtered = qresult.hit_filter(desc_filter)
        >>> len(filtered)
        39
        >>> print(filtered[:4])
        Program: blastn (2.2.27+)
          Query: 33211 (61)
                 mir_1
         Target: refseq_rna
           Hits: ----  -----  ----------------------------------------------------------
                    #  # HSP  ID + description                                          
                 ----  -----  ----------------------------------------------------------
                    0      1  gi|262205317|ref|NR_030195.1|  H**o sapiens microRNA 52...
                    1      2  gi|262205330|ref|NR_030198.1|  H**o sapiens microRNA 52...
                    2      1  gi|262205302|ref|NR_030191.1|  H**o sapiens microRNA 51...
                    3      1  gi|262205451|ref|NR_030222.1|  H**o sapiens microRNA 51...

        Note that instance attributes (other than the hits) from the unfiltered
        QueryResult are retained in the filtered object.

            >>> qresult.program == filtered.program
            True
            >>> qresult.target == filtered.target
            True

        """
        hits = list(filter(func, self.hits))
        obj = self.__class__(hits, self.id, self._hit_key_function)
        self._transfer_attrs(obj)
        return obj

    def hit_map(self, func=None):
        """Creates a new QueryResult object, mapping the given function to its
        Hits.

        Arguments:
        func -- Callback function that accepts a Hit object as its parameter and
                also returns a Hit object.

        Here is an example of using `hit_map` with a function that discards all
        HSPs in a Hit except for the first one:

        >>> from SAP.Bio import SearchIO
        >>> qresult = next(SearchIO.parse('Blast/mirna.xml', 'blast-xml'))
        >>> print(qresult[:8])
        Program: blastn (2.2.27+)
          Query: 33211 (61)
                 mir_1
         Target: refseq_rna
           Hits: ----  -----  ----------------------------------------------------------
                    #  # HSP  ID + description                                          
                 ----  -----  ----------------------------------------------------------
                    0      1  gi|262205317|ref|NR_030195.1|  H**o sapiens microRNA 52...
                    1      1  gi|301171311|ref|NR_035856.1|  Pan troglodytes microRNA...
                    2      1  gi|270133242|ref|NR_032573.1|  Macaca mulatta microRNA ...
                    3      2  gi|301171322|ref|NR_035857.1|  Pan troglodytes microRNA...
                    4      1  gi|301171267|ref|NR_035851.1|  Pan troglodytes microRNA...
                    5      2  gi|262205330|ref|NR_030198.1|  H**o sapiens microRNA 52...
                    6      1  gi|262205302|ref|NR_030191.1|  H**o sapiens microRNA 51...
                    7      1  gi|301171259|ref|NR_035850.1|  Pan troglodytes microRNA...

        >>> top_hsp = lambda hit: hit[:1]
        >>> mapped_qresult = qresult.hit_map(top_hsp)
        >>> print(mapped_qresult[:8])
        Program: blastn (2.2.27+)
          Query: 33211 (61)
                 mir_1
         Target: refseq_rna
           Hits: ----  -----  ----------------------------------------------------------
                    #  # HSP  ID + description                                          
                 ----  -----  ----------------------------------------------------------
                    0      1  gi|262205317|ref|NR_030195.1|  H**o sapiens microRNA 52...
                    1      1  gi|301171311|ref|NR_035856.1|  Pan troglodytes microRNA...
                    2      1  gi|270133242|ref|NR_032573.1|  Macaca mulatta microRNA ...
                    3      1  gi|301171322|ref|NR_035857.1|  Pan troglodytes microRNA...
                    4      1  gi|301171267|ref|NR_035851.1|  Pan troglodytes microRNA...
                    5      1  gi|262205330|ref|NR_030198.1|  H**o sapiens microRNA 52...
                    6      1  gi|262205302|ref|NR_030191.1|  H**o sapiens microRNA 51...
                    7      1  gi|301171259|ref|NR_035850.1|  Pan troglodytes microRNA...

        """
        hits = [deepcopy(hit) for hit in self.hits]
        if func is not None:
            hits = [func(x) for x in hits]
        obj = self.__class__(hits, self.id, self._hit_key_function)
        self._transfer_attrs(obj)
        return obj

    def hsp_filter(self, func=None):
        """Creates a new QueryResult object whose HSP objects pass the filter
        function.

        `hsp_filter` is the same as `hit_filter`, except that it filters
        directly on each HSP object in every Hit. If the filtering removes
        all HSP objects in a given Hit, the entire Hit will be discarded. This
        will result in the QueryResult having less Hit after filtering.

        """
        hits = [x for x in (hit.filter(func) for hit in self.hits) if x]
        obj = self.__class__(hits, self.id, self._hit_key_function)
        self._transfer_attrs(obj)
        return obj

    def hsp_map(self, func=None):
        """Creates a new QueryResult object, mapping the given function to its
        HSPs.

        `hsp_map` is the same as `hit_map`, except that it applies the given
        function to all HSP objects in every Hit, instead of the Hit objects.

        """
        hits = [x for x in (hit.map(func) for hit in list(self.hits)[:]) if x]
        obj = self.__class__(hits, self.id, self._hit_key_function)
        self._transfer_attrs(obj)
        return obj

    # marker for default self.pop() return value
    # this method is adapted from Python's built in OrderedDict.pop
    # implementation
    __marker = object()

    def pop(self, hit_key=-1, default=__marker):
        """Removes the specified hit key and return the Hit object.

        Arguments:
        hit_key -- Integer index or string of hit key that points to a Hit
                   object.
        default -- Value that will be returned if the Hit object with the
                   specified index or hit key is not found.

        By default, `pop` will remove and return the last Hit object in the
        QueryResult object. To remove specific Hit objects, you can use its
        integer index or hit key.

        >>> from SAP.Bio import SearchIO
        >>> qresult = next(SearchIO.parse('Blast/mirna.xml', 'blast-xml'))
        >>> len(qresult)
        100
        >>> for hit in qresult[:5]:
        ...     print(hit.id)
        ... 
        gi|262205317|ref|NR_030195.1|
        gi|301171311|ref|NR_035856.1|
        gi|270133242|ref|NR_032573.1|
        gi|301171322|ref|NR_035857.1|
        gi|301171267|ref|NR_035851.1|

        # remove the last hit
        >>> qresult.pop()
        Hit(id='gi|397513516|ref|XM_003827011.1|', query_id='33211', 1 hsps)

        # remove the first hit
        >>> qresult.pop(0)
        Hit(id='gi|262205317|ref|NR_030195.1|', query_id='33211', 1 hsps)

        # remove hit with the given ID
        >>> qresult.pop('gi|301171322|ref|NR_035857.1|')
        Hit(id='gi|301171322|ref|NR_035857.1|', query_id='33211', 2 hsps)

        """
        # if key is an integer (index)
        # get the ID for the Hit object at that index
        if isinstance(hit_key, int):
            # raise the appropriate error if there is no hit
            if not self:
                raise IndexError("pop from empty list")
            hit_key = list(self.hit_keys)[hit_key]

        try:
            return self._items.pop(hit_key)
        except KeyError:
            # if key doesn't exist and no default is set, raise a KeyError
            if default is self.__marker:
                raise KeyError(hit_key)
        # if key doesn't exist but a default is set, return the default value
        return default

    def index(self, hit_key):
        """Returns the index of a given hit key, zero-based.

        Arguments:
        hit_key -- Hit ID string to look up.

        This method is useful for finding out the integer index (usually
        correlated with search rank) of a given hit key.

        >>> from SAP.Bio import SearchIO
        >>> qresult = next(SearchIO.parse('Blast/mirna.xml', 'blast-xml'))
        >>> qresult.index('gi|301171259|ref|NR_035850.1|')
        7

        """
        if isinstance(hit_key, Hit):
            return list(self.hit_keys).index(hit_key.id)
        return list(self.hit_keys).index(hit_key)

    def sort(self, key=None, reverse=False, in_place=True):
        # no cmp argument to make sort more Python 3-like
        """Sorts the Hit objects.

        Arguments:
        key -- Function used to sort the Hit objects.
        reverse -- Boolean, whether to reverse the sorting or not.
        in_place -- Boolean, whether to perform sorting in place (in the same
                    object) or not (creating a new object).

        `sort` defaults to sorting in-place, to mimick Python's `list.sort`
        method. If you set the `in_place` argument to False, it will treat
        return a new, sorted QueryResult object and keep the initial one
        unsorted.

        """
        if key is None:
            # if reverse is True, reverse the hits
            if reverse:
                sorted_hits = list(self.hits)[::-1]
            # otherwise (default options) make a copy of the hits
            else:
                sorted_hits = list(self.hits)[:]
        else:
            sorted_hits = sorted(self.hits, key=key, reverse=reverse)

        # if sorting is in-place, don't create a new QueryResult object
        if in_place:
            new_hits = OrderedDict()
            for hit in sorted_hits:
                new_hits[self._hit_key_function(hit)] = hit
            self._items = new_hits
        # otherwise, return a new sorted QueryResult object
        else:
            obj = self.__class__(sorted_hits, self.id, self._hit_key_function)
            self._transfer_attrs(obj)
            return obj