def main(self):
appcli.load(self)
library = Library()
entry = library.find_entry(self.protocol)
cmd = os.environ['EDITOR'], entry.path
subp.run(cmd)
def main(self):
appcli.load(self)
library = Library()
entries = library.find_entries(self.protocol)
for entry in entries:
print(entry.path)
def main(self):
appcli.load(self)
try:
if self.command in self.commands:
app = self.commands[self.command]
app.quiet = self.quiet
app.force_text = self.force_text
# Remove top-level options (e.g. --quiet) from the command
# line, so the subcommand doesn't have to deal with them.
sys.argv = [sys.argv[0], self.command, *self.args]
app.main()
else:
io_cli = ProtocolIO()
if self.command:
io_cli = ProtocolIO.from_library(self.command, self.args)
if self.quiet and not io_cli.errors:
io_cli.protocol.clear_footnotes()
# It's more performant to load the protocol specified on the CLI
# before trying to read a protocol from stdin. The reason is
# subtle, and has to do with the way pipes work. For example,
# consider the following pipeline:
#
# sw pcr ... | sw kld ...
#
# Although the output from `sw pcr` is input for `sw kld`, the two
# commands are started by the shell at the same time and run
# concurrently. However, `sw kld` will be forced to wait if it
# tries to read from stdin before `sw pcr` has written to stdout.
# With this in mind, it make sense for `sw kld` to do as much work
# as possible before reading from stdin.
io_stdin = ProtocolIO.from_stdin()
io_stdout = ProtocolIO.merge(io_stdin, io_cli)
io_stdout.to_stdout(self.force_text)
sys.exit(io_stdout.errors)
except KeyboardInterrupt:
print()
except StepwiseError as err:
err.terminate()
def main(cls):
self = cls.from_params()
appcli.load(self)
if not self.output:
if os.fork() != 0:
sys.exit()
df, extras = self.load()
fig = self.plot(df, extras)
if self.output:
out = self.output.replace('$', self.layout_toml.stem)
plt.savefig(out)
plt.close()
else:
plt.show()
def main(self):
appcli.load(self)
library = Library()
entries = library.find_entries(self.protocol)
indent = ' ' if self.organize_by_dir else ''
for collection, entry_group in groupby(entries,
lambda x: x.collection):
if self.organize_by_dir:
print(collection.name)
if self.dirs_only:
continue
for entry in entry_group:
print(indent + entry.name)
if self.organize_by_dir:
print()
def main(self):
appcli.load(self)
print("""\
yotta Y 10²⁴
zetta Z 10²¹
exa E 10¹⁸
peta P 10¹⁵
tera T 10¹²
giga G 10⁹
mega M 10⁶
kilo k 10³
10⁰
milli m 10⁻³
micro μ 10⁻⁶
nano n 10⁻⁹
pico p 10⁻¹²
femto f 10⁻¹⁵
atto a 10⁻¹⁸
zepto z 10⁻²¹
yocto y 10⁻²⁴""")
def main(self):
appcli.load(self)
io = ProtocolIO.from_stdin()
if io.errors:
fatal("protocol has errors, not adding footnote.")
if not io.protocol:
fatal("no protocol specified.")
p = io.protocol
footnote = self.text if self.wrap else pre(self.text)
pattern = re.compile(self.where or '(?=[.:])')
try:
p.insert_footnotes(footnote, pattern=pattern)
except ValueError:
fatal(f"pattern {pattern!r} not found in protocol.")
p.merge_footnotes()
io.to_stdout(self.force_text)
default=100,
)
def get_protocol(self):
p = stepwise.Protocol()
p += pl(
"Remove unligated linker by ultrafiltration:",
s := ul(
"Bring reaction to 500 µL with 8M urea.",
f"Load onto a {self.mwco_kDa} kDa MWCO spin-filter [1].",
"Spin 14000g, 15 min.",
"Wash with 500 µL 8M urea.",
"Wash with 500 µL nuclease-free water.",
"Wash with water again.",
"Wash with water again, but spin for 30 min.",
"Invert the filter into a clean tube and spin 1000g, 2 min to collect ligated product in a volume of ≈15 µL.",
),
)
if self.volume_uL > 15:
s += f"Dilute to {self.volume_uL:g} µL with nuclease-free water"
p.footnotes[1] = 'https://tinyurl.com/4ffxu8zb'
p.footnotes[2] = 'Final urea concentration: ≈200 µM'
return p
if __name__ == '__main__':
app = WashBarendt.from_params()
appcli.load(app)
app.protocol.print()
def main(self):
appcli.load(self)
# Defer importing `sqlalchemy`.
from . import model
with model.open_db() as db:
if self.ls:
model.list_protocols(
db,
categories=self.categories,
dependencies=self.dependencies,
include_dependents=self.show_dependents,
include_complete=self.show_all,
)
elif self.edit:
model.edit_protocol(
db, self.id,
protocol=protocol_from_stdin(),
message=self.message,
categories=self.categories,
dependencies=self.dependencies,
explicit=self.explicit,
)
elif self.peek:
model.peek_protocol(
db, self.id,
quiet=self.quiet,
force_text=self.force_text,
)
elif self.pop:
model.pop_protocol(
db, self.id,
quiet=self.quiet,
force_text=self.force_text,
)
elif self.drop:
model.drop_protocols(db, self.ids)
elif self.restore:
model.restore_protocols(db, self.ids)
elif self.clear:
model.clear_protocols(db)
elif self.reset:
model.reset_protocols(db)
else:
protocol = protocol_from_stdin()
if not protocol:
if self.add: fatal("no protocol specified.")
model.list_protocols(
db,
categories=self.categories,
dependencies=self.dependencies,
include_dependents=self.show_dependents,
include_complete=self.show_all,
)
else:
model.add_protocol(
db, protocol,
message=self.message,
categories=self.categories,
dependencies=self.dependencies,
)
