def do_labels(self, labels):
'''Run whatever transformation on the given labels matrix'''
if (self.operation in (O_SHRINK, O_SHRINK_INF) and
self.wants_fill_holes.value):
labels = fill_labeled_holes(labels)
if self.operation == O_SHRINK_INF:
return binary_shrink(labels)
elif self.operation == O_SHRINK:
return binary_shrink(labels, iterations = self.iterations.value)
elif self.operation in (O_EXPAND, O_EXPAND_INF):
if self.operation == O_EXPAND_INF:
distance = np.max(labels.shape)
else:
distance = self.iterations.value
background = labels == 0
distances, (i,j) = distance_transform_edt(background,
return_indices = True)
out_labels = labels.copy()
mask = (background & (distances <= distance))
out_labels[mask] = labels[i[mask],j[mask]]
return out_labels
elif self.operation == O_DIVIDE:
#
# A pixel must be adjacent to some other label and the object
# must not disappear.
#
adjacent_mask = adjacent(labels)
thinnable_mask = binary_shrink(labels, 1) != 0
out_labels = labels.copy()
out_labels[adjacent_mask & ~ thinnable_mask] = 0
return out_labels
elif self.operation == O_SKELETONIZE:
return skeletonize_labels(labels)
elif self.operation == O_SPUR:
return spur(labels, iterations=self.iterations.value)
else:
raise NotImplementedError("Unsupported operation: %s" %
self.operation.value)
def do_labels(self, labels):
'''Run whatever transformation on the given labels matrix'''
if (self.operation in (O_SHRINK, O_SHRINK_INF) and
self.wants_fill_holes.value):
labels = fill_labeled_holes(labels)
if self.operation == O_SHRINK_INF:
return binary_shrink(labels)
elif self.operation == O_SHRINK:
return binary_shrink(labels, iterations = self.iterations.value)
elif self.operation in (O_EXPAND, O_EXPAND_INF):
if self.operation == O_EXPAND_INF:
distance = np.max(labels.shape)
else:
distance = self.iterations.value
background = labels == 0
distances, (i,j) = distance_transform_edt(background,
return_indices = True)
out_labels = labels.copy()
mask = (background & (distances <= distance))
out_labels[mask] = labels[i[mask],j[mask]]
return out_labels
elif self.operation == O_DIVIDE:
#
# A pixel must be adjacent to some other label and the object
# must not disappear.
#
adjacent_mask = adjacent(labels)
thinnable_mask = binary_shrink(labels, 1) != 0
out_labels = labels.copy()
out_labels[adjacent_mask & ~ thinnable_mask] = 0
return out_labels
elif self.operation == O_SKELETONIZE:
return skeletonize_labels(labels)
elif self.operation == O_SPUR:
return spur(labels, iterations=self.iterations.value)
else:
raise NotImplementedError("Unsupported operation: %s" %
self.operation.value)
def test_01_02_run(self):
module = instantiate_module(MODULE_NAME)
module.input_objects_name.value = INPUT_OBJECTS_NAME
module.output_objects_name.value = OUTPUT_OBJECTS_NAME
module.module_num = 1
pipeline = cpp.Pipeline()
pipeline.add_module(module)
object_set = cpo.ObjectSet()
#
# Pick a bunch of random points, dilate them using the distance
# transform and then label the result.
#
r = np.random.RandomState()
r.seed(12)
bimg = np.ones((100, 100), bool)
bimg[r.randint(0,100, 50), r.randint(0, 100, 50)] = False
labels, count = label(distance_transform_edt(bimg) <= 5)
#
# Make the input objects
#
input_objects = cpo.Objects()
input_objects.segmented = labels
expected = skeletonize_labels(labels)
object_set.add_objects(input_objects, INPUT_OBJECTS_NAME)
#
# Make the workspace
#
workspace = cpw.Workspace(pipeline, module, None, object_set,
cpmeas.Measurements(), None)
module.run(workspace)
self.assertTrue(OUTPUT_OBJECTS_NAME in object_set.object_names,
"Could not find the output objects in the object set")
output_objects = object_set.get_objects(OUTPUT_OBJECTS_NAME)
np.testing.assert_array_equal(expected, output_objects.segmented)
def test_01_02_run(self):
module = instantiate_module(MODULE_NAME)
module.input_objects_name.value = INPUT_OBJECTS_NAME
module.output_objects_name.value = OUTPUT_OBJECTS_NAME
module.module_num = 1
pipeline = cpp.Pipeline()
pipeline.add_module(module)
object_set = cpo.ObjectSet()
#
# Pick a bunch of random points, dilate them using the distance
# transform and then label the result.
#
r = np.random.RandomState()
r.seed(12)
bimg = np.ones((100, 100), bool)
bimg[r.randint(0, 100, 50), r.randint(0, 100, 50)] = False
labels, count = label(distance_transform_edt(bimg) <= 5)
#
# Make the input objects
#
input_objects = cpo.Objects()
input_objects.segmented = labels
expected = skeletonize_labels(labels)
object_set.add_objects(input_objects, INPUT_OBJECTS_NAME)
#
# Make the workspace
#
workspace = cpw.Workspace(pipeline, module, None, object_set,
cpmeas.Measurements(), None)
module.run(workspace)
self.assertTrue(OUTPUT_OBJECTS_NAME in object_set.object_names,
"Could not find the output objects in the object set")
output_objects = object_set.get_objects(OUTPUT_OBJECTS_NAME)
np.testing.assert_array_equal(expected, output_objects.segmented)
def run(self, workspace):
m = workspace.measurements
objects = workspace.object_set.get_objects(self.objects_name.value)
assert isinstance(objects, cpo.Objects)
labels = objects.segmented
benchmarks = dict([(x, np.zeros(objects.count, int))
for x in M_ALL])
ii, jj = np.mgrid[0: objects.shape[0], 0:objects.shape[1]]
benchmarks[M_INTEGER_PLANAR_RAW][:] = np.prod(labels.shape) * 2 / objects.count
b = libtiff.tif_lzw.encode(labels)
benchmarks[M_INTEGER_PLANAR][:] = len(b) / objects.count
#
# Do distance transform in pieces in case objects touch
#
distance = np.zeros(objects.shape, np.float32)
clabels = color_labels(labels)
for i in range(1, np.max(clabels)+1):
mask = clabels == i
distance[mask] = distance_transform_edt(mask)[mask]
maskj = np.zeros((objects.shape[0], objects.shape[1]+1), bool)
skel = skeletonize_labels(labels)
skel_composite = []
rle_composite = []
ij_composite = []
for i in range(1, objects.count+1):
mask = labels == i
maskj[:, :-1] = mask
(imin, imax), (jmin, jmax) = [[f(d) for f in (np.min, np.max)]
for d in (ii[mask], jj[mask])]
#
####################
#
# Binary planar
#
benchmarks[M_BINARY_PLANAR_RAW][i-1] = (imax - imin + 1) * (jmax - jmin + 1)
b = libtiff.tif_lzw.encode(mask[imin:(imax+1), jmin:(jmax+1)])
#
# Compressed + 16-bit origin & size
#
benchmarks[M_BINARY_PLANAR][i-1] = len(b) + 8
#
####################
#
# RLE encoded
#
fm = (~ maskj[:, :-1]) & maskj[:, 1:]
lm = maskj[:, :-1] & ~ maskj[:, 1:]
iif = ii[fm]
jjf = jj[fm] + 1
iil = ii[lm]
jjl = jj[lm] + 1
order = np.lexsort((iif, jjf))
iif, jjf = iif[order], jjf[order]
order = np.lexsort((iil, jjl))
iil, jjl = iil[order], jjl[order]
data = np.column_stack((iif, jjf, jjl - jjf + 1))
benchmarks[M_RLE_RAW][i-1] = np.prod(data.shape) * 2
rle_composite.append(data)
b = self.compress((data[:, 0], data[:, 1], data[:, 2]))
benchmarks[M_RLE][i-1] = len(b)
#
###################
#
# IJ
#
data = np.column_stack((ii[mask], jj[mask]))
order = np.lexsort((jj[mask], ii[mask]))
data = data[order, :]
ij_composite.append(data)
benchmarks[M_IJ_RAW][i-1] = np.prod(data.shape) * 2
b = self.compress((data[:, 0], data[:, 1]))
benchmarks[M_IJ][i-1] = len(b)
skmask = skel == i
iii = ii[skmask]
jjj = jj[skmask]
ddd = distance[skmask]
order = np.lexsort((jjj, iii))
data = np.rec.array((iii[order], jjj[order], ddd[order]),
dtype = [("i", np.uint16), ("j", np.uint16),
("d", np.float32)])
benchmarks[M_SKEL_RAW][i-1] = len(data.tostring())
b = self.compress((data["i"], data["j"], data["d"]))
benchmarks[M_SKEL][i-1] = len(b)
skel_composite.append(data)
for data, idxs, ftr in (
(np.vstack(ij_composite).transpose(), (0, 1), M_IJ_COMPOSITE),
(np.vstack(rle_composite).transpose(), (0, 1, 2), M_RLE_COMPOSITE),
(np.hstack(skel_composite), ("i", "j", "d"), M_SKEL_COMPOSITE)):
b = self.compress([data[idx] for idx in idxs])
benchmarks[ftr][:] = len(b) / objects.count + 4
for key in benchmarks:
m.add_measurement(self.objects_name.value, key, benchmarks[key])
def test_01_02_run(self):
module = instantiate_module(MODULE_NAME)
module.input_objects_name.value = INPUT_OBJECTS_NAME
module.output_objects_name.value = OUTPUT_OBJECTS_NAME
module.module_num = 1
pipeline = cpp.Pipeline()
pipeline.add_module(module)
object_set = cpo.ObjectSet()
#
# Pick a bunch of random points, dilate them using the distance
# transform and then label the result.
#
r = np.random.RandomState()
r.seed(12)
bimg = np.ones((100, 100), bool)
bimg[r.randint(0,100, 50), r.randint(0, 100, 50)] = False
labels, count = label(distance_transform_edt(bimg) <= 5)
#
# Make the input objects
#
input_objects = cpo.Objects()
input_objects.segmented = labels
expected = skeletonize_labels(labels)
object_set.add_objects(input_objects, INPUT_OBJECTS_NAME)
#
# Make the workspace
#
measurements = cpmeas.Measurements()
workspace = cpw.Workspace(pipeline, module, None, object_set,
measurements, None)
module.run(workspace)
#
# Calculate the centers using Numpy. Scipy can do this too.
# But maybe it's instructive to show you how to go at the labels
# matrix using Numpy.
#
# We're going to get the centroids by taking the average value
# of x and y per object.
#
y, x = np.mgrid[0:labels.shape[0], 0:labels.shape[1]].astype(float)
#
# np.bincount counts the number of occurrences of each integer value.
# You need to operate on a 1d array - if you flatten the labels
# and weights, their pixels still align.
#
# We do [1:] to discard the background which is labeled 0
#
# The optional second argument to np.bincount is the "weight". For
# each label value, maintain a running sum of the weights.
#
areas = np.bincount(expected.flatten())[1:]
total_x = np.bincount(expected.flatten(), weights=x.flatten())[1:]
total_y = np.bincount(expected.flatten(), weights=y.flatten())[1:]
expected_location_x = total_x / areas
expected_location_y = total_y / areas
#
# Now check against the measurements.
#
count_feature = I.C_COUNT + "_" + OUTPUT_OBJECTS_NAME
self.assertTrue(measurements.has_feature(cpmeas.IMAGE, count_feature),
"Your module did not produce a %s measurement" %
count_feature)
count = measurements.get_measurement(cpmeas.IMAGE, count_feature)
self.assertEqual(count, len(areas))
for ftr, expected in ((I.M_LOCATION_CENTER_X, expected_location_x),
(I.M_LOCATION_CENTER_Y, expected_location_y)):
self.assertTrue(measurements.has_feature(
OUTPUT_OBJECTS_NAME, ftr))
location = measurements.get_measurement(OUTPUT_OBJECTS_NAME, ftr)
np.testing.assert_almost_equal(location, expected)
def run(self, workspace):
#
# This is unfortunate... sorry. example4b.py is imported during the
# import of cellprofiler.modules. This means that the import of
# cellprofiler.modules.identify can't be done in this module's import
#
import cellprofiler.modules.identify as I
#
# The object_set keeps track of the objects produced during a cycle
#
# Crucial methods:
#
# object_set.get_objects(name) returns an instance of cpo.Objects
#
# object_set.add(objects, name) adds objects with the given name to
# the object set
#
#
# Create objects in three steps:
# make a labels matrix
# create an instance of cpo.Objects()
# set cpo.Objects.segmented = labels matrix
#
# You can be "nicer" by giving more information, but this is not
# absolutely necessary. See subsequent exercises for how to be nice.
#
object_set = workspace.object_set
input_objects = object_set.get_objects(self.input_objects_name.value)
labels = skeletonize_labels(input_objects.segmented)
output_objects = cpo.Objects()
output_objects.segmented = labels
output_objects_name = self.output_objects_name.value
object_set.add_objects(output_objects, output_objects_name)
##measurements = workspace.measurements
#
# The cpo.Objects has several useful properties that are calculated
# and remembered: count and area are the ones most frequently used.
# count is the # of objects in the segmentation
#
##n_objects = output_objects.count
#
# cellprofiler.module.identify has some helper methods for adding
# measurements in a standardized fashion. add_object_count_measurements
# only adds one measurement: Count_<objects-name>
#
##I.add_object_count_measurements(measurements, output_objects_name,
## n_objects)
#
# cellprofiler.modules.identify.add_object_location_measurements
# computes the center of mass for each object in the labels matrix
# and records those in the object measurement, Location_Center_X
# and Location_Center_Y. These measurements are used by data mining
# programs such as CellProfiler Analyst to center an image on a
# particular cell.
#
##I.add_object_location_measurements(
## measurements, output_objects_name, labels, n_objects)
if workspace.show_frame:
workspace.display_data.input_labels = input_objects.segmented
workspace.display_data.output_labels = labels