def calculate_bin_coverage(self, bin):
"""
Take a single bin, return a matrix. This is passed to a multiprocessing Pool.
:param bin: Bin should be passed as "Chr19_4343343"
:return: pd.DataFrame with rows containing NaNs dropped
"""
# Get reads from bam file
parser = BamFileReadParser(self.input_bam_file, 20, self.mbias_read1_5, self.mbias_read1_3,
self.mbias_read2_5, self.mbias_read2_3, self.no_overlap)
# Split bin into parts
chromosome, bin_location = bin.split("_")
bin_location = int(bin_location)
try:
reads = parser.parse_reads(chromosome, bin_location-self.bin_size, bin_location)
matrix = parser.create_matrix(reads)
except BaseException as e:
# No reads are within this window, do nothing
self.bins_no_reads += 1
return None
except:
logging.error("Unknown error: {}".format(bin))
return None
# drop rows of ALL NaN
matrix = matrix.dropna(how="all")
# convert to data_frame of 1s and 0s, drop rows with NaN
matrix = matrix.dropna()
# if matrix is empty, attempt to create it with correction before giving up
if len(matrix) == 0:
original_matrix = matrix.copy()
reads = parser.correct_cpg_positions(reads)
try:
matrix = parser.create_matrix(reads)
except InvalidIndexError as e:
logging.error("Invalid Index error when creating matrices at bin {}".format(bin))
logging.debug(str(e))
return bin, original_matrix
except ValueError as e:
logging.error("Matrix concat error ar bin {}".format(bin))
logging.debug(str(e))
matrix = matrix.dropna()
if len(matrix) > 0:
logging.info("Correction attempt at bin {}: SUCCESS".format(bin))
else:
logging.info("Correction attempt at bin {}: FAILED".format(bin))
return bin, matrix
def _multiprocess_extract(self, one_bin: str):
"""Function to be used for multiprocessing
Arguments:
one_bin {str} -- bin id as "chr7_222222"
Returns:
[tuple] -- bin, matrix
"""
try:
read_parser = BamFileReadParser(self.bam_file,
20,
read1_5=self.mbias_read1_5,
read1_3=self.mbias_read1_3,
read2_5=self.mbias_read2_5,
read2_3=self.mbias_read2_3)
chrom, loc = one_bin.split("_")
loc = int(loc)
reads = read_parser.parse_reads(chrom, loc - 100,
loc) # TODO unhardcode bin size
matrix = read_parser.create_matrix(reads)
matrix = matrix.dropna(how="all")
# if matrix.shape[0] == 0:
# return None
matrix = matrix.fillna(-1)
matrix = np.array(matrix)
matrix = matrix.astype('int8')
except: # BAD EXCEPTION
return (one_bin, np.array([]))
return (one_bin, matrix)
def process_bins(self, bin):
"""
This is the main method and should be called using Pool.map It takes one bin location and uses the other helper
functions to get the reads, form the matrix, cluster it with DBSCAN, and output the cluster data as text lines
ready to writing to a file.
:param bin: string in this format: "chr19_55555"
:return: a list of lines representing the cluster data from that bin
"""
try:
chromosome, bin_loc = bin.split("_")
except ValueError:
return None
bin_loc = int(bin_loc)
# Create bam parser and parse reads
bam_parser_A = BamFileReadParser(self.bam_a,
20,
read1_5=self.mbias_read1_5,
read1_3=self.mbias_read1_3,
read2_5=self.mbias_read2_5,
read2_3=self.mbias_read2_3,
no_overlap=self.no_overlap)
reads_A = bam_parser_A.parse_reads(chromosome, bin_loc - self.bin_size,
bin_loc)
if not self.single_file_mode:
bam_parser_B = BamFileReadParser(self.bam_b,
20,
read1_5=self.mbias_read1_5,
read1_3=self.mbias_read1_3,
read2_5=self.mbias_read2_5,
read2_3=self.mbias_read2_3,
no_overlap=self.no_overlap)
reads_B = bam_parser_B.parse_reads(chromosome,
bin_loc - self.bin_size,
bin_loc)
# This try/catch block returns None for a bin if any discrepancies in the data format of the bins are detected.
# The Nones are filtered out during the output of the data
try:
# create matrix drop NA
# This matrix is actually a pandas dataframe
matrix_A = bam_parser_A.create_matrix(reads_A).dropna()
# Attempt to correct CpG Position if necessary
if len(matrix_A) == 0:
reads_A = self.attempt_cpg_position_correction(
reads_A, bam_parser_A)
matrix_A = bam_parser_A.create_matrix(reads_A).dropna()
if not self.single_file_mode:
matrix_B = bam_parser_B.create_matrix(reads_B).dropna()
# attempt to correct CpG position in B if necessary
if len(matrix_B) == 0:
reads_B = self.attempt_cpg_position_correction(
reads_B, bam_parser_B)
matrix_B = bam_parser_B.create_matrix(reads_B).dropna()
except ValueError as e:
logging.error(
"ValueError when creating matrix at bin {}. Stack trace will be below if log level=DEBUG"
.format(bin))
logging.debug(str(e))
return None
except InvalidIndexError as e:
logging.error(
"Invalid Index error when creating matrices at bin {}".format(
bin))
logging.debug(str(e))
return None
# if read depths are still not a minimum, skip
if matrix_A.shape[0] < self.read_depth_req:
return None
if not self.single_file_mode:
if matrix_B.shape[0] < self.read_depth_req:
return None
# create labels and add to dataframe
# If two files label each A and B, otherwise use file_name as label
if not self.single_file_mode:
labels_A = ['A'] * len(matrix_A)
matrix_A['input'] = labels_A
labels_B = ['B'] * len(matrix_B)
matrix_B['input'] = labels_B
else:
labels_A = [os.path.basename(self.bam_a)] * len(matrix_A)
matrix_A['input'] = labels_A
if not self.single_file_mode:
try:
# ensure they have the same CpG positions
matrix_B.columns = matrix_A.columns
full_matrix = pd.concat([matrix_A, matrix_B], sort=False)
except ValueError as e:
logging.error("Matrix concat error in bin {}".format(bin))
# logging.debug(str(e))
return None
else:
full_matrix = matrix_A
# Get data without labels for clustering
data_to_cluster = np.array(full_matrix)[:, :-1]
# Create DBSCAN classifier and cluster add cluster classes to df
clf = DBSCAN(min_samples=2)
try:
labels = clf.fit_predict(data_to_cluster)
except ValueError as e:
# log error
logging.error(
"ValueError when trying to cluster bin {}".format(bin))
logging.debug(str(e))
return None
full_matrix['class'] = labels
# Filter out any clusters with less than a minimum
filtered_matrix = self.filter_data_frame(full_matrix)
if self.remove_noise:
filtered_matrix = filtered_matrix[filtered_matrix['class'] != -1]
# return generate_output_data(filtered_matrix, chromosome, bin_loc)
return self.generate_individual_matrix_data(filtered_matrix,
chromosome, bin_loc)