def __init__(self):
self.path_home = Constants.PATH_HOME
self.protein_list_file = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_PROTEIN_FILE_PROPERTY, True)
self.protein_list = FileParser.read_file(self.protein_list_file)
self.motif_folder = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_FOLDER_PROPERTY, True)
self.domain_region_file = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_DOMAIN_REGION_FILE_PROPERTY, True)
def dictionary_identifier(self):
Logger.get_instance().info(
" Creation of a dictionary for novel gene of dataset 2\
The dictionary structure is : \n \
{gene = [ isoform1, isoform2,...isoformN]}")
self.ensembl_path_output = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.ENSEMBL_OUTPUT_PATH_SEQUENCE_PROPERTY,
True)
self.ensembl_output_dataset2 = self.ensembl_path_output + PropertyManager.get_instance(
).get_property(DataConstants.ENSEMBL_FILE_SEQUENCES_2_PROPERTY, True)
self.dictionary_output = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.DICTIONARY_PATH_OUTPUT_PROPERTY, True)
self.dictionary_namefile = self.dictionary_output + PropertyManager.get_instance(
).get_property(DataConstants.DICTIONARY_NAME_FILE_PROPERTY, True)
dict_identifier = InfoFasta.make_dictionary(
self.ensembl_output_dataset2)
file_dict = FileUtils.open_text_w(self.dictionary_namefile)
pickle.dump(dict_identifier, file_dict)
Logger.get_instance().info(
" The creation of a dictionary for novel gene in dataset 2 is completed \n\n"
)
def iupred_motifs(self):
Logger.get_instance().info( " .....Start of IUPred motifs analysis.....\n")
self.iupred_folder = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_IUPRED_FOLDER_PROPERTY,True)
# Iupred Analysis at threshold value of 0.4
Timer.get_instance().step(" Start of IUPred motifs analysis - threshold = 0.4 \n")
self.threshold_1 = Constants.MOTIFS_THRESHOLD_1
self.output_folder_1 = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_IUP_OUTPUT_FOLDER_1_PROPERTY, True)
GlobalOverlapRegionAnalysis.iupred_overlap_analysis(self.protein_list,self.iupred_folder, self.output_folder_1, self.threshold_1,
self.motif_folder,self.domain_region_file)
Timer.get_instance().step(" End of IUPred motifs analysis - threshold = 0.4 \n")
# Iupred Analysis at threshold value of 0.5
Timer.get_instance().step(" Start of IUPred motifs analysis - threshold = 0.5 \n")
self.threshold_2 = Constants.MOTIFS_THRESHOLD_2
self.output_folder_2 = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_IUP_OUTPUT_FOLDER_2_PROPERTY, True)
GlobalOverlapRegionAnalysis.iupred_overlap_analysis(self.protein_list,self.iupred_folder, self.output_folder_2, self.threshold_2,
self.motif_folder,self.domain_region_file)
Timer.get_instance().step(" End of IUPred motifs analysis - threshold = 0.5 \n")
Logger.get_instance().info( " .....End of IUPred motifs analysis.....\n")
def make_dictionary(self):
Logger.get_instance().info(
" Creation of a dictionary for novel gene of dataset 2\
The dictionary structure is : \n \
{gene = [ isoform1, isoform2,...isoformN]}")
self.path_home = Constants.PATH_HOME
self.path_input_file = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_DICTIONARY_INPUT_FILE_PROPERTY,
True)
self.dictionary_output_path = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_DICTIONARY_OUTPUT_PATH_PROPERTY,
True)
self.output_file_path = self.dictionary_output_path + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_DICTIONARY_FILE_OUTPUT_PROPERTY,
True)
dict_identifier = InfoFasta.make_dictionary(self.path_input_file)
self.dict_file = FileUtils.open_text_w(self.output_file_path)
pickle.dump(dict_identifier, self.dict_file)
Logger.get_instance().info(
" The creation of a dictionary is completed \n\n")
def delet_append_file(self):
self.del_ensembl_input = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.DEL_ENSEMBL_PATH_PROPERTY, True)
self.del_ensembl_file1 = self.del_ensembl_input + PropertyManager.get_instance(
).get_property(DataConstants.DEL_ENSEMBL_FILE1_PROPERTY, True)
self.del_ensembl_file2 = self.del_ensembl_input + PropertyManager.get_instance(
).get_property(DataConstants.DEL_ENSEMBL_FILE2_PROPERTY, True)
self.del_longest_input = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.DEL_LONGEST_PATH_PROPERTY, True)
self.del_longest_file = self.del_longest_input + PropertyManager.get_instance(
).get_property(DataConstants.DEL_LONGEST_FILE_PROPERTY, True)
self.del_isoform_file = self.del_longest_input + PropertyManager.get_instance(
).get_property(DataConstants.DEL_ISOFORM_FILE_PROPERTY, True)
self.del_random_isoform_file = self.del_longest_input + PropertyManager.get_instance(
).get_property(DataConstants.DEL_RANDOM_ISOFORM_FILE_PROPERTY, True)
self.del_fusion_path = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.DEL_FUSION_PATH_PROPERTY, True)
self.del_fusion_file_longest = self.del_fusion_path + PropertyManager.get_instance(
).get_property(DataConstants.DEL_FUSION_DATASET_LONGEST_PROPERTY, True)
self.del_fusion_file_dataset12 = self.del_fusion_path + PropertyManager.get_instance(
).get_property(DataConstants.DEL_FUSION_DATASET12_PROPERTY, True)
files = [
self.del_ensembl_file1, self.del_ensembl_file2,
self.del_longest_file, self.del_isoform_file,
self.del_random_isoform_file, self.del_fusion_file_longest,
self.del_fusion_file_dataset12
]
for namefile in files:
RemoveFile.delete_file(namefile)
def isoform_sequences(self):
Logger.get_instance().info(
" Starting the random selection of isoforms with same length \n")
Logger.get_instance().info(
" The following headers are the proteins randomly selected \n")
self.path_output_longest = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_PATH_OUTPUT_PROPERTY, True)
self.path_file_isoform = self.path_output_longest + PropertyManager.get_instance(
).get_property(DataConstants.ISOFORM_FILE_PROPERTY, True)
self.path_file_selected_isoform = self.path_output_longest + PropertyManager.get_instance(
).get_property(DataConstants.RANDOM_ISOFORM_SEQ_PROPERTY, True)
# The headers of a Isoform fasta file are taken by InfoFasta class
# You make sure that the arg text is equal to False because the input object is a file and not a list
self.headers = InfoFasta.get_header(self.path_file_isoform, text=False)
# Extraction of genes form headers line
# This vector contains double gene because the file contains some isoform of the same gene
gene_isoform = []
for header in self.headers:
gene = header[1:16]
gene_isoform.append(gene)
# gene set creation
unique_gene = set(gene_isoform)
# This for loop flows on the unique gene
#
random_header = []
old_num_isoform = 0
for gene in unique_gene:
# For each gene counts how many isoform has
num_isoform = gene_isoform.count(gene)
item = range(0, num_isoform)
# Select one isoform randomly
sel = random.choice(item)
# The header selected randomly are stored in array
random_header.append(self.headers[old_num_isoform:old_num_isoform +
num_isoform][sel])
old_num_isoform = old_num_isoform + num_isoform
self.file_random_seq = FileUtils.open_text_a(
self.path_file_selected_isoform)
# The sequences corresponding to header selected are extracted from isoform file
for header in random_header:
Logger.get_instance().info('Header selected : ' + header)
identifier = header[33:48]
sequence = InfoFasta.get_seq(self.path_file_isoform, identifier)
fasta_seq = SeqToFasta.give_fasta(header, sequence)
self.file_random_seq.write(fasta_seq)
Logger.get_instance().info(" End of selection random sequences \n ")
def iupred_analysis(self):
Timer.get_instance().step(" Start of Iupred analysis...")
self.tool_path_input = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.TOOL_PATH_INPUT_PROPERTY, True)
self.iupred_path_output = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.IUPRED_PATH_OUTPUT_PROPERTY,True)
Iupred.global_iupred_analysis(self.tool_path_input,
self.iupred_path_output)
Timer.get_instance().step(" End of Iupred analysis")
def anchor_analysis(self):
Timer.get_instance().step(" Start of Anchor analysis...")
self.tool_path_input = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.TOOL_PATH_INPUT_PROPERTY, True)
self.anchor_path_output = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.ANCHOR_PATH_OUTPUT_PROPERTY,True)
self.motif_list_path = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.ANCHOR_MOTIF_PATH_PROPERTY, True)
Anchor.global_anchor_analysis(self.motif_list_path,
self.tool_path_input,
self.anchor_path_output)
Timer.get_instance().step(" End of Anchor analysis")
def disordpbind_analysis(self):
Timer.get_instance().step(" Start of DisoRDPbind output analysis.. ")
self.path_home = Constants.PATH_HOME
self.input_file = self.path_home + PropertyManager.get_instance().get_property( DataConstants.DISO_INPUT_FILE_PROPERTY, True)
self.ouput_path = self.path_home + PropertyManager.get_instance().get_property( DataConstants.DISO_OUTPUT_FOLDER_PROPERTY, True)
self.binding_partner = PropertyManager.get_instance().get_property( DataConstants.DISO_BINDING_PARTNER_PROPERTY, True)
self.num_aa_diso = PropertyManager.get_instance().get_property( DataConstants.DISO_NUM_AA_PROPERTY, True)
self.dataset_type = PropertyManager.get_instance().get_property( DataConstants.DISO_DATASET_TYPE_PROPERTY, True)
DisoRDPbind.make_disordp_file(self.input_file, self.ouput_path, int(self.binding_partner), int(self.num_aa_diso), self.dataset_type)
Timer.get_instance().step(" End of DisoRDPbind output analysis")
def anchor_motifs(self):
Logger.get_instance().info( " .....Start of IUPred motifs analysis.....\n")
self.anchor_folder = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_ANCHOR_FOLDER_PROPERTY, True)
self.anchor_output_folder = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_ANCHOR_OUTPUT_FOLDER_PROPERTY, True)
Timer.get_instance().step(" Start of ANCHOR motifs analysis \n")
GlobalOverlapRegionAnalysis.anchor_overlap_analysis(self.protein_list,self.anchor_folder,self.anchor_output_folder,
self.motif_folder,self.domain_region_file)
Timer.get_instance().step(" End of IUPred motifs analysis \n")
Logger.get_instance().info( " .....End of ANCHOR motifs analysis.....\n")
def split_dataset(self):
Logger.get_instance().info( " Division of Dataset in many fasta file each containing one protein sequence")
self.path_home = Constants.PATH_HOME
self.split_path_input = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPLIT_PATH_INPUT_PROPERTY, True)
self.split_file_fasta = self.split_path_input + PropertyManager.get_instance().get_property( DataConstants.SPLIT_DATASET_PROPERTY, True)
self.split_path_output = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.SPLIT_PATH_OUTPUT_PROPERTY, True)
self.split_start_index = PropertyManager.get_instance().get_property( DataConstants.SPLIT_START_HEADER_PROPERTY, True)
self.split_end_index = PropertyManager.get_instance().get_property( DataConstants.SPLIT_END_HEADER_PROPERTY, True)
SplitSeq.split_seq( self.split_file_fasta, self.split_path_output, int(self.split_start_index), int(self.split_end_index))
Logger.get_instance().info( " The dataset has been split in many fasta files ")
def disordp_motifs(self):
Logger.get_instance().info( " .....Start of DisoRDPbind motifs analysis.....\n")
self.disordp_folder = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_DISORDP_FOLDER_PROPERTY, True)
self.disordp_output_folder = self.path_home + PropertyManager.get_instance().get_property( DataConstants.MOTIFS_DISORDP_OUTPUT_FOLDER_PROPERTY, True)
self.filename = PropertyManager.get_instance().get_property( DataConstants.MOTIFS_DISORDP_FILE_PROPERTY,True)
Timer.get_instance().step(" Start of DisoRDPbind motifs analysis \n")
GlobalOverlapRegionAnalysis.disordp_overlap_analysis(self.protein_list, self.disordp_folder, self.filename, self.motif_folder,
self.domain_region_file,self.disordp_output_folder)
Timer.get_instance().step(" End of DisoRDPbind motifs analysis \n")
Logger.get_instance().info( " .....End of DisoRDPbind motifs analysis.....\n")
def download_product_gene_seq(self):
Logger.get_instance().info(
" Start of sequences download from Ensembl..")
self.path_home = Constants.PATH_HOME
self.gene_list_input = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_ENSEMBL_FILE_INPUT_LIST_PROPERTY,
True)
self.ensembl_seq_output = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_ENSEMBL_FILE_OUPUT_SEQ_PROPERTY,
True)
self.type_query = PropertyManager.get_instance().get_property(
DataConstants.DOWNLOAD_ENSEMBL_TYPE_QUERY_PROPERTY, True)
Ensembl.list_get_seq(self.gene_list_input,
int(self.type_query),
path_output=self.ensembl_seq_output)
Logger.get_instance().info(" End of sequences download from Ensembl..")
def merger_sequences(self):
Logger.get_instance().info(
" Union of the longest sequences and the random selected isoform ")
# Input variables to merge the longest Novel sequences and random selected isoform of dataset
self.path_home = Constants.PATH_HOME
self.path_file_longest = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_FUSION_FILE_LONGEST_PROPERTY,
True)
self.path_file_random = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_FUSION_FILE_RANDOM_PROPERTY,
True)
self.path_final_file = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_FUSION_FINAL_DATASET_PROPERTY,
True)
FileParser.merge_file(self.path_file_longest, self.path_file_random,
self.path_final_file)
Logger.get_instance().info(
" The Final Proteome Dataset has been created\n ")
def whole_procedure():
dataset_type = PropertyManager.get_instance().get_property( DataConstants.MOTIFS_DATASET_TYPE_PROPERTY, True)
# start chrono
Timer.get_instance().start_chrono()
Logger.get_instance().info(" ........Start of " + dataset_type + " Motifs Analysis.....\n ")
motifs = MotifsAnalysis()
motifs.iupred_motifs()
motifs.anchor_motifs()
motifs.disordp_motifs()
Timer.get_instance().stop_chrono(" End of " + dataset_type + " Motifs Analysis")
def get_longest_seq(self):
Logger.get_instance().info(
" Start of the selection of longest sequences of dataset \n")
self.path_home = Constants.PATH_HOME
self.file_sequences = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_LONGEST_SEQ_INPUT_FILE_PROPERTY,
True)
self.output_path = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_LONGEST_SEQ_OUTPUT_PATH_PROPERTY,
True)
self.dictionary_file = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_LONGEST_DICTIONARY_PROPERTY,
True)
self.longest_file = self.output_path + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_LONGEST_SEQ_FILE_PROPERTY, True)
self.isoform_file = self.output_path + PropertyManager.get_instance(
).get_property(DataConstants.DOWNLOAD_LONGEST_ISOFORM_FILE, True)
# Extraction the longest sequences from dataset sequences (isoforms)
self.file_seq = open(self.file_sequences)
self.seq_obj = self.file_seq.readlines()
LengthSeq.longest_seq(self.seq_obj,
self.dictionary_file,
self.longest_file,
self.isoform_file,
type_obj='list')
Logger.get_instance().info(
" End of selection of the longest sequences: \n \
two file have been generated one with longest sequences and the other one containing the isoform with same length "
)
def comparison_dataset(self):
# Create Logger instance to see the start of comparison between two datasets
Logger.get_instance().info(" Start of comparison datasets:...")
# Definition of InfoDataset arguments
self.dataset_input_path = PropertyManager.get_instance().get_property(
DataConstants.DATASET_INPUT_PATH_PROPERTY, True)
self.dataset_1_file = PropertyManager.get_instance().get_property(
DataConstants.DATASET_1_FILE_PROPERTY, True)
self.dataset_2_file = PropertyManager.get_instance().get_property(
DataConstants.DATASET_2_FILE_PROPERTY, True)
self.dataset_1_index_col = PropertyManager.get_instance().get_property(
DataConstants.DATASET_1_INDEX_COL_PROPERTY, True)
self.dataset_2_index_col = PropertyManager.get_instance().get_property(
DataConstants.DATASET_2_INDEX_COL_PROPERTY, True)
self.dataset_output = PropertyManager.get_instance().get_property(
DataConstants.DATASET_OUTPUT_PROPERTY, True)
self.dataset_1_length = PropertyManager.get_instance().get_property(
DataConstants.DATASET_1_LENGTH_PROPERTY, True)
self.dataset_2_length = PropertyManager.get_instance().get_property(
DataConstants.DATASET_2_LENGTH_PROPERTY, True)
self.index_col = (int(self.dataset_1_index_col),
int(self.dataset_2_index_col))
self.dataset_length = (int(self.dataset_1_length),
int(self.dataset_2_length))
self.path_home = Constants.PATH_HOME
InfoDataset.global_analysis_dataset(
self.path_home + self.dataset_input_path,
(self.dataset_1_file, self.dataset_2_file),
self.index_col,
self.path_home + self.dataset_output,
length=self.dataset_length)
Logger.get_instance().info(
" The comparison of datasets is completed : \
two file with the common and difference \
items has been generated in \n\n " + self.dataset_output)
def analysis_tools_output(self):
Timer.get_instance().step(" Start of tools analysis.. ")
self.path_home = Constants.PATH_HOME
self.input_path_iupred = self.path_home + PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_INPUT_PATH_IUPRED_PROPERTY, True)
self.output_path_analysis = self.path_home + PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_OUTPUT_PATH_TOOLS_PROPERTY, True)
self.threshold_1 = PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_THRESHOLD_1_PROPERTY, True)
self.threshold_2 = PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_THRESHOLD_2_PROPERTY, True)
self.number_aa_iupred = PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_AMINOACID_NUMBER_IUPRED_PROPERTY, True)
self.dataset_type = PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_DATASET_TYPE_PROPERTY, True)
Iupred.make_iupred_file(self.input_path_iupred, self.output_path_analysis, float(self.threshold_1), float(self.threshold_2), int(self.number_aa_iupred), self.dataset_type)
self.input_path_anchor = Constants.PATH_HOME + PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_INPUT_PATH_ANCHOR_PROPERTY, True)
self.num_aa_anchor = PropertyManager.get_instance().get_property( DataConstants.ANALYSIS_AMINOACID_NUMBER_ANCHOR_PROPERTY, True)
Anchor.make_anchor_file(self.input_path_anchor, self.output_path_analysis, int(self.num_aa_anchor),self.dataset_type )
Timer.get_instance().step(" End of tools analysis")
def change_header(self):
# Variables definition
self.path_home = Constants.PATH_HOME
self.path_input = self.path_home + PropertyManager.get_instance().get_property( DataConstants.HEADER_INPUT_SEQ_PROPERTY, True)
self.namefile = PropertyManager.get_instance().get_property( DataConstants.HEADER_FILE_SEQ_PROPERTY, True)
self.path_file_input = self.path_input + self.namefile
self.path_output = self.path_home + PropertyManager.get_instance().get_property( DataConstants.HEADER_OUTPUT_SEQ_PROPERTY, True)
self.path_file_output = self.path_output + PropertyManager.get_instance().get_property( DataConstants.HEADER_FILE_OUTPUT_PROPERTY, True)
self.source = PropertyManager.get_instance().get_property( DataConstants.HEADER_SOURCE_PROPERTY, True)
self.type_id = PropertyManager.get_instance().get_property( DataConstants.HEADER_TYPE_ID_PROPERTY,True)
# Method calling
HeaderParser.change_header(self.path_file_input, self.path_file_output, source=int(self.source), type_id=int(self.type_id))
def longest_sequence(self):
Logger.get_instance().info(
" Start of the selection of longest sequences of novel dataset \n")
# Definition of arguments
self.path_sequences = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_PATH_SEQUENCE_PROPERTY, True)
self.file_sequences = self.path_sequences + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_PROT_FILE_SEQUENCES_2_PROPERTY,
True)
self.path_dictionary_identifier = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_PATH_DICTIONARY_PROPERTY, True)
self.file_dictionary = self.path_dictionary_identifier + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_DICTIONARY_NAME_FILE_PROPERTY,
True)
self.path_output_longest = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_PATH_OUTPUT_PROPERTY, True)
self.path_file_longest = self.path_output_longest + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_FILE_PROPERTY, True)
self.path_file_isoform = self.path_output_longest + PropertyManager.get_instance(
).get_property(DataConstants.ISOFORM_FILE_PROPERTY, True)
# Extraction the longest sequences from dataset 2 sequences (isoforms)
LengthSeq.longest_seq(self.file_sequences, self.file_dictionary,
self.path_file_longest, self.path_file_isoform)
# Timer step
Timer.get_instance().step(
" End of selection of the longest sequences in dataset 2 \n")
Logger.get_instance().info(
" End of selection of the longest sequences: \n \
two file have been generated one with longest sequences and the other one containing the isoform with same length "
)
def creation_list(self):
Logger.get_instance().info(" Creation of gene and protein list \n ")
# Creation of two file containing respectively the genes and protein of dataset 1
self.dataset_input_path = PropertyManager.get_instance().get_property(
DataConstants.DATASET_INPUT_PATH_PROPERTY, True)
self.file_dataset_1 = PropertyManager.get_instance().get_property(
DataConstants.DATASET_1_FILE_PROPERTY, True)
self.gene_index_col = PropertyManager.get_instance().get_property(
DataConstants.LIST_GENE_INDEX_COL_PROPERTY, True)
self.protein_index_col = PropertyManager.get_instance().get_property(
DataConstants.LIST_PROTEIN_INDEX_COL_PROPERTY, True)
self.list_gene_dataset_1 = PropertyManager.get_instance().get_property(
DataConstants.LIST_FILE_GENE_DATASET_1_PROPERTY, True)
self.list_protein_dataset_1 = PropertyManager.get_instance(
).get_property(DataConstants.LIST_FILE_PROTEIN_DATASET_1_PROPERTY,
True)
self.path_gene_dataset_1 = Constants.PATH_HOME + self.dataset_input_path + self.list_gene_dataset_1
self.path_protein_dataset_1 = Constants.PATH_HOME + self.dataset_input_path + self.list_protein_dataset_1
self.path_home = Constants.PATH_HOME
self.path_dataset_1 = self.path_home + self.dataset_input_path + self.file_dataset_1
dataset_1 = FileParser.make_table(self.path_dataset_1)
gene_dataset_1 = TableWrapper.get_column(dataset_1,
int(self.gene_index_col),
start=1)
protein_dataset_1 = TableWrapper.get_column(
dataset_1, int(self.protein_index_col), start=1)
FileWriter.write_table(self.path_gene_dataset_1, gene_dataset_1)
FileWriter.write_table(self.path_protein_dataset_1, protein_dataset_1)
Logger.get_instance().info(
" The genes and proteins file of dataset 1 have been created \
in the following path \n\n " + self.dataset_input_path)
def domain_classification(self):
self.path_home = Constants.PATH_HOME
self.file_domain = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_LIST_FILE_PROPERTY, True)
self.file_jprot_information = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_LIST_JPROTEOMICS_PROPERTY, True)
self.file_pfamid = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_FILE_PFAM_PROPERTY, True)
# reading of pfam table in particular of the pfam id and the domain name
table_pfam = FileParser.make_table(self.file_pfamid)
pfamid = TableWrapper.get_column(table_pfam, 0)
domain_name = TableWrapper.get_column(table_pfam, 3)
# dictionary pfamid and domain name
dict_pfam = {row[3]: row[0] for row in table_pfam}
dict_pfam['DUF1785'] = '-'
dict_pfam['DUF1898'] = '-'
# reading of domain classification provided by dataset 2
# dictionary motifs--> class
table_domain = FileParser.make_table(self.file_domain)
dict_type_domain = TableWrapper.make_dictionary(table_domain)
# make a inverse dictionary class--> motifs
inverse_table_domain = TableWrapper.inv_column(table_domain, 0, 1)
dict_class_domain = TableWrapper.make_dictionary(inverse_table_domain)
# reading of domain information of dataset 2
jprot_table = FileParser.make_table(self.file_jprot_information,
skip=1)
for i, item in enumerate(jprot_table):
if len(item) == 1:
jprot_table[i] = [item[0], '.', '.']
# Jproteomics
# ===================================
# extraction of domain for each gene
# extraction of gene id
domain_column_jprot = TableWrapper.get_column(jprot_table, 1)
genes_jprot = TableWrapper.get_column(jprot_table, 0)
pfam_id_jprot = TableWrapper.get_column(jprot_table, 2)
# This part reads the type of domain for each gene and creates a new column with the type of domain
# at the end returns a new table with namegene, domain name, pfam id, class of domain
#
# One gene/protein can have more than one domain
# For each gene the following part checks if the classification of protein domains by comparing the domain with dict_class_domains:
#
#
count_classical = 0
count_nonclassic = 0
count_unclissified = 0
count_other_class = 0
count_no_domain = 0
new_table_jprot = []
for i, gene in enumerate(genes_jprot):
row = []
row.append(gene)
row.append(domain_column_jprot[i])
row.append(pfam_id_jprot[i])
string_domains = ''
# If the protein hasn't any domain, add static 'no-domains' information
if domain_column_jprot[i] == '.':
string_domains += Constants.DOMAIN_NONE + Constants.DOMAIN_COMMA
count_no_domain += 1
# If the protein contains some domains checks the class and
# makes a string containing the class domains separated by a comma
else:
domains = domain_column_jprot[i].split(',')
for type_domain in domains:
print type_domain
if type_domain in dict_type_domain:
class_domain = dict_type_domain[type_domain][0]
if class_domain == Constants.DOMAIN_CLASSICAL:
string_domains += Constants.DOMAIN_CLASSICAL + Constants.DOMAIN_COMMA
count_classical += 1
elif class_domain == Constants.DOMAIN_NONCLASSICAL:
string_domains += Constants.DOMAIN_NONCLASSICAL + Constants.DOMAIN_COMMA
count_nonclassic += 1
elif class_domain == Constants.DOMAIN_UNKNOWN:
string_domains += Constants.DOMAIN_UNKNOWN + Constants.DOMAIN_COMMA
count_unclissified += 1
elif type_domain not in dict_type_domain:
string_domains += Constants.DOMAIN_OTHER + Constants.DOMAIN_COMMA
count_other_class += 1
else:
Logger.get_instance().info('unexpected case',
type_domain)
# -1 allows to delete the last comma in string
row.append(string_domains[0:len(string_domains) - 1])
new_table_jprot.append(row)
# print of proteins number for each domain class
Logger.get_instance().info(str(count_classical))
Logger.get_instance().info(str(count_nonclassic))
Logger.get_instance().info(str(count_unclissified))
Logger.get_instance().info(str(count_other_class))
Logger.get_instance().info(str(count_no_domain))
self.path_ouput_file_jprot = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_FINAL_TABLE_JPROT_PROPERTY, True)
FileWriter.write_table(self.path_ouput_file_jprot, new_table_jprot)
# NatRevGenetics
# =========================================================
# this part classifies the gene in according to the type of domain and creates a new table with
# name gene, type domain, pfam id, clas of domain
self.input_file_nrgenetics = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_LIST_NATREVGENETICS_PROPERTY, True)
# reading of domain information of dataset 2
nrgenetics_table = FileParser.make_table(self.input_file_nrgenetics,
skip=1)
# extraction of domain domain for each gene
# extraction of gene id
domain_column_nrgenetics = TableWrapper.get_column(nrgenetics_table, 2)
genes_nrgenetics = TableWrapper.get_column(nrgenetics_table, 0)
# This part reads the type of domain for each gene and creates a new columns with the type of domain
# at the end returns a new table with namegene, domain name, pfam id , class of domain
#
# One gene/protein can have more than one domain
# For each gene the following part checks if the classification of protein domains by comparing the domain with dict_class_domains:
#
#
count_classical = 0
count_nonclassic = 0
count_unclissified = 0
count_other_class = 0
count_no_domain = 0
new_table_nrgenetics = []
for i, gene in enumerate(genes_nrgenetics):
row = []
row.append(gene)
row.append(domain_column_nrgenetics[i])
string_domains = ''
string_pfamid = ''
# If the protein hasn't any domain, add static 'no-domains' information
if domain_column_nrgenetics[i] == '.':
string_domains += Constants.DOMAIN_NONE + Constants.DOMAIN_COMMA
string_pfamid += '.,'
count_no_domain += 1
# If the protein contains some domains checks the class and
# akes a string containing the class domains separated by a comma
else:
# the domains are separated by a comma
domains = domain_column_nrgenetics[i].split(',')
for type_domain in domains:
# the domain present also the number information
# X-domain[n] for this reason in order to take only the domain name
# the domain is split to '['
type_domain = type_domain.split('[')[0]
if type_domain in dict_pfam:
string_pfamid += dict_pfam[type_domain] + ','
else:
string_pfamid += '-,'
print type_domain
if type_domain in dict_type_domain:
class_domain = dict_type_domain[type_domain][0]
if class_domain == Constants.DOMAIN_CLASSICAL:
string_domains += Constants.DOMAIN_CLASSICAL + Constants.DOMAIN_COMMA
count_classical += 1
elif class_domain == Constants.DOMAIN_NONCLASSICAL:
string_domains += Constants.DOMAIN_NONCLASSICAL + Constants.DOMAIN_COMMA
count_nonclassic += 1
elif class_domain == Constants.DOMAIN_UNKNOWN:
string_domains += Constants.DOMAIN_UNKNOWN + Constants.DOMAIN_COMMA
count_unclissified += 1
elif type_domain not in dict_type_domain:
string_domains += Constants.DOMAIN_OTHER + Constants.DOMAIN_COMMA
count_other_class += 1
else:
print 'unexpected case', type_domain
row.append(string_pfamid[0:len(string_pfamid) - 1])
row.append(string_domains[0:len(string_domains) - 1])
new_table_nrgenetics.append(row)
# print of proteins number for each domain class
Logger.get_instance().info(str(count_classical))
Logger.get_instance().info(str(count_nonclassic))
Logger.get_instance().info(str(count_unclissified))
Logger.get_instance().info(str(count_other_class))
Logger.get_instance().info(str(count_no_domain))
self.path_ouput_file_nrgenetics = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_FINAL_TABLE_NATREVGENETICS, True)
FileWriter.write_table(self.path_ouput_file_nrgenetics,
new_table_nrgenetics)
# reading of sequences file in order to take the headers
# reading of gene and protein id correspondences for different genes
self.file_sequences = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_FILE_SEQ_PROPERTY, True)
self.header = InfoFasta.get_header(self.file_sequences, text=False)
gene_seq = [item.split('>')[1].split('|')[0] for item in self.header]
prot_seq = [item.split('>')[1].split('|')[2] for item in self.header]
# Construction of table containing the RBP protein and the corresponding class domain and pfam id
count_j = 0
count_n = 0
final_table = []
title = [
'gene id', 'type_domain', 'pfam id', 'class domain', 'prot id'
]
final_table.append(title)
# table construction
for n, gene in enumerate(gene_seq):
Logger.get_instance().info(str(n + 1))
Logger.get_instance().info(gene)
# if gene is in jprot and in nrgenetics
if gene in genes_jprot and gene in genes_nrgenetics:
count_n += 1
ind_gene = genes_nrgenetics.index(gene)
row = new_table_nrgenetics[ind_gene]
row.append(prot_seq[n])
# if gene is in jprot and not in nrgenetics
elif gene in genes_jprot and gene not in genes_nrgenetics:
count_j += 1
ind_gene = genes_jprot.index(gene)
row = new_table_jprot[ind_gene]
row.append(prot_seq[n])
# if gene not in jprot and gene in nrgenetics
elif gene not in genes_jprot and gene in genes_nrgenetics:
count_n += 1
ind_gene = genes_nrgenetics.index(gene)
row = new_table_nrgenetics[ind_gene]
row.append(prot_seq[n])
# if the gene is not in both dataset: Error
else:
Logger.get_instance().info('Error' + gene + prot_seq[n])
final_table.append(row)
sort_table = TableWrapper.inv_column(final_table, 0, 4)
sort_table = TableWrapper.inv_column(sort_table, 1, 4)
sort_table = TableWrapper.inv_column(sort_table, 2, 4)
sort_table = TableWrapper.inv_column(sort_table, 3, 4)
self.final_file_table = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.DOMAIN_FINALE_TABLE_RBP_DATASET_PROPERTY,
True)
FileWriter.write_table(self.file_final_table, sort_table)
# Counting the number of protein with Classical domain, Non-classical, unclassified or combinations of this class
prot_name = TableWrapper.get_column(sort_table, 1, 1)
class_domain = TableWrapper.get_column(sort_table, 4, 1)
classical = []
nonclassical = []
unclissified = []
otherclass = []
nodomain = []
# there are several combinations of class domain in a protein
# the list creation in according to class domain has been performed by the filters of spreadsheet (see documentation)
for n, domain in enumerate(class_domain):
diff_domain = domain.split(',')
for item in diff_domain:
if item == Constants.DOMAIN_CLASSICAL:
classical.append(prot_name[n])
elif item == Constants.DOMAIN_NONCLASSICAL:
nonclassical.append(prot_name[n])
elif item == Constants.DOMAIN_UNKNOWN:
unclissified.append(prot_name[n])
elif item == Constants.DOMAIN_OTHER:
otherclass.append(prot_name[n])
elif item == Constants.DOMAIN_NONE:
nodomain.append(prot_name[n])
else:
# many others
pass
@staticmethod
def whole_procedure():
dataset_type = PropertyManager.get_instance().get_property( DataConstants.MOTIFS_DATASET_TYPE_PROPERTY, True)
# start chrono
Timer.get_instance().start_chrono()
Logger.get_instance().info(" ........Start of " + dataset_type + " Motifs Analysis.....\n ")
motifs = MotifsAnalysis()
motifs.iupred_motifs()
motifs.anchor_motifs()
motifs.disordp_motifs()
Timer.get_instance().stop_chrono(" End of " + dataset_type + " Motifs Analysis")
if __name__ == '__main__':
OptionManager.get_instance().initialize()
# Set the level of verbosity
Logger.get_instance().set_level( OptionManager.get_instance().get_option( OptionConstants.OPTION_VERBOSITY))
PropertyManager.get_instance().read_properties( OptionManager.get_instance().get_option( OptionConstants.OPTION_MOTIFS_ANALYSIS_PROPERTY_PATH, True))
MotifsAnalysis.whole_procedure()
Timer.get_instance().start_chrono()
Logger.get_instance().info(
"Start of the creation of RBP dataset.....\n ")
M = MakeDatasetRbp()
#M.delet_append_file()
#M.comparison_dataset()
#M.creation_list()
#M.connection_to_ensembl()
#M.dictionary_identifier()
#M.longest_sequence()
#M.isoform_sequences()
#M.merger_sequences()
M.split_dataset()
Timer.get_instance().stop_chrono(' End of the creation of RBP dataset')
if __name__ == '__main__':
OptionManager.get_instance().initialize()
# Retrieve the MakeDatasetRbp properties
PropertyManager.get_instance().read_properties(
OptionManager.get_instance().get_option(
OptionConstants.OPTION_MAKEDATASETRBP_PROPERTIES_PATH, True))
MakeDatasetRbp.whole_procedure()
def connection_to_ensembl(self):
Logger.get_instance().info(" Connection to Ensembl: Starting...\n")
# DATASET 1
# =============================================
# Collection of sequences for dataset 1
Logger.get_instance().info(" Dataset 1 : Extraction of sequences...\n")
# Timer step
Timer.get_instance().step(" Start of sequences extraction \n")
# Definition of Ensembl list_get_seq arguments
self.path_home = Constants.PATH_HOME
self.list_path = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.DATASET_INPUT_PATH_PROPERTY, True)
self.gene_list_1 = PropertyManager.get_instance().get_property(
DataConstants.LIST_FILE_GENE_DATASET_1_PROPERTY, True)
self.protein_list = PropertyManager.get_instance().get_property(
DataConstants.LIST_FILE_PROTEIN_DATASET_1_PROPERTY, True)
self.ensembl_gene_list_1_path = self.list_path + self.gene_list_1
self.ensembl_protein_list_1_path = self.list_path + self.protein_list
self.type_query1_ensembl = PropertyManager.get_instance().get_property(
DataConstants.ENSEMBL_TYPE_QUERY_DATASET_1_PROPERTY, True)
self.ensembl_path_output = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.ENSEMBL_OUTPUT_PATH_SEQUENCE_PROPERTY,
True)
self.ensembl_output_dataset1 = self.ensembl_path_output + PropertyManager.get_instance(
).get_property(DataConstants.ENSEMBL_FILE_SEQUENCES_1_PROPERTY)
# Calling Ensembl.list_get_seq
Ensembl.list_get_seq(
self.ensembl_gene_list_1_path,
int(self.type_query1_ensembl),
path_protein_list=self.ensembl_protein_list_1_path,
path_output=self.ensembl_output_dataset1)
# Timer step
Timer.get_instance().step(" End of Dataset 1 Sequences Extraction\n")
Logger.get_instance().info(
" Extraction of sequences for the dataset 1 has been completed \n\n"
)
# END DATASET 1
# =====================================================
# DATASET 2
# =====================================================
# Collection of sequences for dataset 2
Logger.get_instance().info(
" Dataset 2 : Extraction of sequences ....\n")
# Definition of Ensembl list_get_seq arguments
self.ensembl_input_list_2 = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.DATASET_OUTPUT_PROPERTY, True)
self.gene_list_2 = Constants.FILE_DIFF
self.ensembl_gene_list_2_path = self.ensembl_input_list_2 + self.gene_list_2
self.type_query2_ensembl = PropertyManager.get_instance().get_property(
DataConstants.ENSEMBL_TYPE_QUERY_DATASET_2_PROPERTY, True)
self.ensembl_path_output = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.ENSEMBL_OUTPUT_PATH_SEQUENCE_PROPERTY,
True)
self.ensembl_output_dataset2 = self.ensembl_path_output + PropertyManager.get_instance(
).get_property(DataConstants.ENSEMBL_FILE_SEQUENCES_2_PROPERTY, True)
# Calling Ensembl.list_get_seq
Ensembl.list_get_seq(self.ensembl_gene_list_2_path,
int(self.type_query2_ensembl),
path_protein_list=None,
path_output=self.ensembl_output_dataset2)
# Timer step
Timer.get_instance().step(" End of Dataset 2 Sequences Extraction\n")
Logger.get_instance().info(
" Extraction of sequences for the dataset 2 has been completed \n\n"
)
# END DATASET 2
# =====================================================
Logger.get_instance().info(
" The sequences file of dataset 1 and the novel gene in dataset 2 \
have been created in the following path \n" + self.ensembl_path_output)
def whole_procedure():
# start chrono
Timer.get_instance().start_chrono()
Logger.get_instance().info("Start of the sequences extraction.....\n ")
D = DownloadEnsemblSeq()
#D.download_product_gene_seq()
#D.make_dictionary()
#D.get_longest_seq()
#D.isoform_sequences()
#D.merger_sequences()
Timer.get_instance().stop_chrono(' End of the sequences extraction')
if __name__ == '__main__':
OptionManager.get_instance().initialize()
# Retrieve the properties DownloadEnsemblSeq
PropertyManager.get_instance().read_properties(
OptionManager.get_instance().get_option(
OptionConstants.OPTION_DOWNLOADENSEMBLSEQ_PROPERTY_PATH, True))
D = DownloadEnsemblSeq()
D.whole_procedure()
def particular_analysis(self):
Timer.get_instance().step(" Start of tools analysis for specific protein ")
self.path_home = Constants.PATH_HOME
self.path_input_anchor_file = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_ANCHOR_FILE_PROPERTY, True)
self.path_input_iupred_file = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_IUPRED_FILE_PROPERTY, True)
self.path_input_disordp_file = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_DISORDP_FILE_PROPERTY, True)
self.path_input_reg_anchor = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_REG_ANCHOR_FILE_PROPERTY, True)
self.path_input_reg_iupred_1 = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_REG_IUPRED_1_FILE_PROPERTY, True)
self.path_input_reg_iupred_2 = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_REG_IUPRED_2_FILE_PROPERTY, True)
self.path_input_reg_diso = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_REG_DISO_FILE_PROPERTY, True)
self.input_files = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_INPUT_DIR_FILE_PROPERTY, True)
self.list_namefiles = PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_LIST_NAMEFILE_PROPERTY, True)
self.path_output_dir = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_OUTPUT_DIR_PROPERTY, True)
self.path_output_dir_diso = self.path_home + PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_OUTPUT_DIR_DISO_PROPERTY, True)
# This parameter represents the column of protein id in the classification files
#
# In Domain Class files the column of protein id is the 2 ( that is 1 for python)
# In RNA target files the column of protein id is the 1 (that is 0 for python)
#
#self.protein_column_rna = PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_PROTEIN_LIST_COLUMN_RNA_PROPERTY, True)
self.protein_column_class = PropertyManager.get_instance().get_property( DataConstants.SPECIFIC_PROTEIN_LIST_COLUMN_CLASS_PROPERTY, True)
# region file
anchor_table = FileParser.make_table(self.path_input_reg_anchor, skip=1)
iupred_table_1 = FileParser.make_table(self.path_input_reg_iupred_1, skip=1)
iupred_table_2 = FileParser.make_table(self.path_input_reg_iupred_2, skip=1)
disordp_table = FileParser.make_table(self.path_input_reg_diso, skip=1)
# table file (fraction)
anchor_t = FileParser.make_table(self.path_input_anchor_file, skip=1)
iupred_t = FileParser.make_table(self.path_input_iupred_file, skip=1)
disordp_t = FileParser.make_table(self.path_input_disordp_file, skip=1)
list_filenames = self.list_namefiles.split(',')
for filename in list_filenames:
feature = filename.split('.')[0]
table_domain = FileParser.make_table(self.input_files + str(filename))
list_prot = TableWrapper.get_column(table_domain,int(self.protein_column_class))
prot_id_anchor = TableWrapper.get_column(anchor_table, 0)
prot_id_iupred_1 = TableWrapper.get_column(iupred_table_1, 0)
prot_id_iupred_2 = TableWrapper.get_column(iupred_table_2, 0)
prot_id_disordp = TableWrapper.get_column(disordp_table, 0)
prot_id_anchor_t = TableWrapper.get_column(anchor_t, 0)
prot_id_iupred_t = TableWrapper.get_column(iupred_t, 0)
prot_id_disordp_t = TableWrapper.get_column(disordp_t, 0)
# region file
new_table_anchor = [line for n, line in enumerate(anchor_table) if prot_id_anchor[n] in list_prot]
new_table_iupred_1 = [line for n, line in enumerate(iupred_table_1) if prot_id_iupred_1[n] in list_prot]
new_table_iupred_2 = [line for n, line in enumerate(iupred_table_2) if prot_id_iupred_2[n] in list_prot]
new_table_disordp = [line for n, line in enumerate(disordp_table) if prot_id_disordp[n] in list_prot]
anchor_output_file_path = self.path_output_dir + feature + '_AnchorRegion.txt'
iupred1_output_file_path = self.path_output_dir + feature + '_IUPredRegion_0.4.txt'
iupred2_output_file_path = self.path_output_dir + feature + '_IUPredRegion_0.5.txt'
disordp_output_file_path = self.path_output_dir_diso + feature + '_DisoRDPRegion.txt'
# Table file (fraction)
new_table_a = [line for n, line in enumerate(anchor_t) if prot_id_anchor_t[n] in list_prot]
new_table_i = [line for n, line in enumerate(iupred_t) if prot_id_iupred_t[n] in list_prot]
new_table_d = [line for n, line in enumerate(disordp_t) if prot_id_disordp_t[n] in list_prot]
anchor_output_table = self.path_output_dir + feature + '_AnchorTable.txt'
iupred_output_table = self.path_output_dir + feature + '_IUPredTable_0.4_0.5.txt'
disordp_output_table = self.path_output_dir_diso + feature + '_DisoRDPTable.txt'
# file writing
# Region file
FileWriter.write_table(anchor_output_file_path, new_table_anchor)
FileWriter.write_table(iupred1_output_file_path, new_table_iupred_1)
FileWriter.write_table(iupred2_output_file_path, new_table_iupred_2)
FileWriter.write_table(disordp_output_file_path, new_table_disordp)
# Table file
FileWriter.write_table(anchor_output_table, new_table_a)
FileWriter.write_table(iupred_output_table, new_table_i)
FileWriter.write_table(disordp_output_table, new_table_d)
Timer.get_instance().step(" End of tools analysis for specific protein ")
def merger_sequences(self):
Logger.get_instance().info(
" Union of the longest sequences and the random selected isoform ")
# Input variables to merge the longest Novel sequences and random selected isoform of dataset 2
self.path_input_longest = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_PATH_INPUT_PROPERTY, True)
self.path_file_longest = self.path_input_longest + PropertyManager.get_instance(
).get_property(DataConstants.LONGEST_FILE_PROPERTY, True)
self.path_file_isoform = self.path_input_longest + PropertyManager.get_instance(
).get_property(DataConstants.SELECTED_ISOFORM_FILE_PROPERTY, True)
self.path_output_seq = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_PATH_OUTPUT_PROPERTY, True)
self.path_file_seq_dataset_2 = self.path_output_seq + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_FILE_SEQ_DATASET_2_PROPERTY, True)
FileParser.merge_file(self.path_file_longest, self.path_file_isoform,
self.path_file_seq_dataset_2)
# Input variables to merge the sequences datasets (Novel_JProteomics and NatRevGenetics)
self.path_input_seq_dataset1 = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_PATH_INPUT_DATASET_1_PROPERTY,
True)
self.path_file_dataset1 = self.path_input_seq_dataset1 + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_FILE_DATASET_1_PROPERTY, True)
self.path_file_dataset12 = self.path_output_seq + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_DATASET_12_PROPERTY, True)
Logger.get_instance().info(
" Union of sequences respectively of dataset 1 and the novel dataset 2 proteins \n "
)
FileParser.merge_file(self.path_file_dataset1,
self.path_file_seq_dataset_2,
self.path_file_dataset12)
Logger.get_instance().info(" The New RBP Dataset has been created\n ")
# This part checks if there are pseudo genes inside dataset 2
# Make the comparison between the original genes gived as an input and the genes obtained after
# connection to Ensembl
# This check allows to find genes that are not anymore available or that are pseudogenes
Logger.get_instance().info(
" Comparison between original genes and Ensembl output ")
self.path_home = Constants.PATH_HOME
self.path_input_original_file = self.dataset_output = PropertyManager.get_instance(
).get_property(DataConstants.DATASET_OUTPUT_PROPERTY, True)
self.original_file = self.path_home + self.path_input_original_file + Constants.FILE_DIFF
original_genes = FileParser.read_file(self.original_file)
self.path_output_seq = Constants.PATH_HOME + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_PATH_OUTPUT_PROPERTY, True)
self.path_file_seq_dataset_2 = self.path_output_seq + PropertyManager.get_instance(
).get_property(DataConstants.FUSION_FILE_SEQ_DATASET_2_PROPERTY, True)
final_headers = InfoFasta.get_header(self.path_file_seq_dataset_2)
final_genes = [item[1:16] for item in final_headers]
out_comparison = InfoDataset.comparison_dataset(original_genes,
final_genes,
header=False)
genes = '\n'.join(out_comparison[1])
Logger.get_instance().info(
" The genes lost during the request to Ensembl are : \n" + genes)
def mrna_protein(self):
self.path_home = Constants.PATH_HOME
self.jproteomics_seq = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.PUTATIVERNA_JPROTEOMICS_SEQ_PROPERTY,
True)
self.jproteomics_info = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.PUTATIVERNA_JPROTEOMICS_INFO_PROPERTY,
True)
# Reading J proteomics file containing information about the protein attendance in others datasets
#
info_table = FileParser.make_table(self.jproteomics_info, '\t', skip=1)
#
# column Extraction
gene_info = TableWrapper.get_column(info_table, 1)
castello = TableWrapper.get_column(info_table, 2)
baltz = TableWrapper.get_column(info_table, 3)
esc = TableWrapper.get_column(info_table, 4)
rbpdb = TableWrapper.get_column(info_table, 5)
rnacompete = TableWrapper.get_column(info_table, 6)
mrna = [(cast_val, baltz_val, esc_val)
for cast_val, baltz_val, esc_val in zip(castello, baltz, esc)]
self.header = InfoFasta.get_header(self.jproteomics_seq, text=False)
# Protein and gene of dataset 2 attend in final RBP dataset
#
gene_seq = [item.split('>')[1].split('|')[0] for item in self.header]
prot_seq = [item.split('>')[1].split('|')[2] for item in self.header]
# Construction of column containing the putative RNA target for each gene
# in according to if condition
putative_rna_target = []
rbpdb_target = []
for n, gene_id in enumerate(gene_seq):
Logger.get_instance().info(gene_id)
ind_gene_id = gene_info.index(gene_id)
# if the gene have at least one Y in this array can be considered to have mRNA target
if 'Y' in mrna[ind_gene_id]:
rna_target = 'mRNA'
Logger.get_instance().info(
'The putative Rna target of this gene is ' + rna_target)
putative_rna_target.append([gene_id, prot_seq[n], rna_target])
elif 'N' in mrna[ind_gene_id]:
# if the gene attend in rnacompete means that the RNA target is unkown
if 'N' in rbpdb[ind_gene_id] and 'Y' in rnacompete[ind_gene_id]:
rna_target = 'unknown'
Logger.get_instance().info(
'The putative Rna target of this gene is ' +
rna_target)
putative_rna_target.append(
[gene_id, prot_seq[n], rna_target])
# if the gene attend in rbpdb means just it is a RBP protein (no information about RNA target)
elif 'Y' in rbpdb[ind_gene_id] and 'N' in rnacompete[
ind_gene_id]:
rna_target = 'RBPDB'
Logger.get_instance().info('The gene is in ' + rna_target)
rbpdb_target.append([gene_id, prot_seq[n], rna_target])
else:
Logger.get_instance().info(' Check this line' +
info_table[ind_gene_id])
self.file_rna_target_jeproteomics = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.PUTATIVE_MRNA_GENE_JPROTEOMICS_PROPERTY,
True)
self.file_rbpdb_jproteomics = self.path_home + PropertyManager.get_instance(
).get_property(DataConstants.PUTATIVE_RBPDB_GENE_JPROTEOMICS_PROPERTY,
True)
# File Writing
FileWriter.write_table(self.file_mrna_jeproteomics,
putative_rna_target,
symbol='\t')
FileWriter.write_table(self.file_rbpdb_jproteomics,
rbpdb_target,
symbol='\t')
@staticmethod
def whole_procedure():
# start chrono
Timer.get_instance().start_chrono()
Logger.get_instance().info("Start of Disorder Analysis.....\n ")
disorder = DisorderAnalysis()
#disorder.change_header()
#disorder.split_dataset()
#disorder.anchor_analysis()
#disorder.iupred_analysis()
#disorder.analysis_tools_output()
#disorder.disordpbind_analysis()
#disorder.particular_analysis()
Timer.get_instance().stop_chrono(' End of Disorder Analysis')
if __name__ == '__main__':
OptionManager.get_instance().initialize()
PropertyManager.get_instance().read_properties( OptionManager.get_instance().get_option( OptionConstants.OPTION_DISORDER_ANALYSIS_PATH, True))
DisorderAnalysis.whole_procedure()
