#Set the mode of the pins (broadcom vs local) GPIO.setmode(GPIO.BCM) #set the enable pins for L and R stepper motors to 1 to prevent overheating GPIO.setup(L_enablePIN, GPIO.OUT, initial=1) GPIO.setup(R_enablePIN, GPIO.OUT, initial=1) #create Stepper class instances for left and right reward delivery water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN) water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN) #create lickometer class instances for left and right lickometers lick_port_L = core.lickometer(L_lickometer) lick_port_R = core.lickometer(R_lickometer) tone_go = core.tones(go_tone_freq, 0.75) camera = PiCamera() #---------------------------- #Initialize experiment #---------------------------- camera.start_preview(rotation=180, fullscreen=False, window=(0, -44, 350, 400)) #Set the time for the beginning of the block trials = np.arange(n_trials) data = core.data(n_trials, mouse_number, block_number) #start L and R reward counters total_reward_L = 0
GPIO.setup(L_enablePIN, GPIO.OUT, initial=1)
GPIO.setup(R_enablePIN, GPIO.OUT, initial=1)
#initialize the mixer (for tones) at the proper sampling rate.
mixer.init(frequency=44100)
#create Stepper class instances for left and right reward delivery
water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN)
water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN)
#create lickometer class instances for left and right lickometers
lick_port_L = core.lickometer(L_lickometer)
lick_port_R = core.lickometer(R_lickometer)
#create instruction tones
lowfreq_singlepulse = core.tones(low_freq, sample_tone_length,
single_pulse_length) #1000Hz single pulse
lowfreq_multipulse = core.tones(low_freq, sample_tone_length,
multi_pulse_length) #1000Hz multi pulse
highfreq_singlepulse = core.tones(high_freq, sample_tone_length,
single_pulse_length) #4000Hz single pulse
highfreq_multipulse = core.tones(high_freq, sample_tone_length,
multi_pulse_length) #4000Hz multi pulse
#create tone that is used as an error signal
tone_wrong = core.tones(wrong_tone_freq, wrong_tone_length, wrong_tone_length)
if ttl_experiment == 'y':
#set up ttl class instances triggers and marker TTL output
TTL_trigger = core.ttl(TTL_trigger_PIN, TTL_pulse_length)
TTL_marker = core.ttl(TTL_marker_PIN, TTL_pulse_length)
GPIO.setup(L_enablePIN, GPIO.OUT, initial=1)
GPIO.setup(R_enablePIN, GPIO.OUT, initial=1)
#initialize the mixer (for tones) at the proper sampling rate.
mixer.init(frequency=44100)
#create Stepper class instances for left and right reward delivery
water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN)
water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN)
#create lickometer class instances for left and right lickometers
lick_port_L = core.lickometer(L_lickometer)
lick_port_R = core.lickometer(R_lickometer)
#create tones
tone_L = core.tones(L_tone_freq, sample_tone_length)
tone_R = core.tones(R_tone_freq, sample_tone_length)
# tone_go = core.tones(go_tone_freq, go_tone_length)
tone_wrong = core.tones(wrong_tone_freq, wrong_tone_length)
if ttl_experiment == 'y':
#set up ttl class instances triggers and marker TTL output
TTL_trigger = core.ttl(TTL_trigger_PIN, TTL_pulse_length)
TTL_marker = core.ttl(TTL_marker_PIN, TTL_pulse_length)
camera = PiCamera() #create camera object
#----------------------------
#Initialize experiment
#----------------------------
GPIO.setup(R_enablePIN, GPIO.OUT, initial = 1) #initialize the mixer (for tones) at the proper sampling rate. mixer.init(frequency = 44100) #create Stepper class instances for left and right reward delivery water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN) water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN) #create lickometer class instances for left and right lickometers lick_port_L = core.lickometer(L_lickometer) lick_port_R = core.lickometer(R_lickometer) #create tones tone_go = core.tones(go_tone_freq, go_tone_length) #create "go" tone camera = PiCamera() #create camera object #---------------------------- #Initialize experiment #---------------------------- camera.start_preview(rotation = 180, fullscreen = False, window = (0,-44,350,400)) #Set the time for the beginning of the block trials = np.arange(n_trials) data = core.data(protocol_description, n_trials, mouse_number, block_number) total_reward_L = 0 total_reward_R = 0
GPIO.setup(L_enablePIN, GPIO.OUT, initial=1) GPIO.setup(R_enablePIN, GPIO.OUT, initial=1) #initialize the mixer (for tones) at the proper sampling rate. mixer.init(frequency=44100) #create stepper class instances for left and right reward delivery water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN) water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN) #create lickometer class instances for left and right lickometers lick_port_L = core.lickometer(L_lickometer) lick_port_R = core.lickometer(R_lickometer) #create tones tone_L = core.tones(L_tone_freq, sample_tone_length) tone_R = core.tones(R_tone_freq, sample_tone_length) tone_go = core.tones(go_tone_freq, go_tone_length) tone_wrong = core.tones(wrong_tone_freq, wrong_tone_length) #set up ttl class instances triggers and marker TTL output TTL_trigger = core.ttl(TTL_trigger_PIN, TTL_pulse_length) TTL_marker = core.ttl(TTL_marker_PIN, TTL_pulse_length) camera = PiCamera() #create camera object #---------------------------- #Initialize experiment #----------------------------
GPIO.setup(L_enablePIN, GPIO.OUT, initial=1)
GPIO.setup(R_enablePIN, GPIO.OUT, initial=1)
#initialize the mixer (for tones) at the proper sampling rate.
mixer.init(frequency=44100)
#create Stepper class instances for left and right reward delivery
water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN)
water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN)
#create lickometer class instances for left and right lickometers
lick_port_L = core.lickometer(L_lickometer)
lick_port_R = core.lickometer(R_lickometer)
#create tones
tone_L = core.tones(L_tone_freq, sample_tone_length) #create left tone
tone_R = core.tones(R_tone_freq, sample_tone_length) #create right tone
tone_go = core.tones(go_tone_freq, go_tone_length) #create "go" tone
camera = PiCamera() #create camera object
camera.resolution = (320, 240) #set picam resolution
camera.rotation = (180) #invert the image
filename = f'{mouse_number}_{date}_block{block_number}.h264'
camera.annotate_text_size = (20)
#----------------------------
#Initialize experiment
#----------------------------
camera.start_preview(rotation=180, fullscreen=False, window=(0, -44, 350, 400))
GPIO.setup(L_enablePIN, GPIO.OUT, initial = 1) GPIO.setup(R_enablePIN, GPIO.OUT, initial = 1) #initialize the mixer (for tones) at the proper sampling rate. mixer.init(frequency = 44100) #create Stepper class instances for left and right reward delivery water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN) water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN) #create lickometer class instances for left and right lickometers lick_port_L = core.lickometer(L_lickometer) lick_port_R = core.lickometer(R_lickometer) #create tones tone_go = core.tones(go_tone_freq, go_tone_length) tone_reward = core.tones(reward_tone_freq, reward_tone_length) #create a reward tone tone_no_reward = core.tones(no_reward_tone_freq, reward_tone_length) #create a "no reward" tone tone_wrong = core.tones(wrong_tone_freq, reward_tone_length) camera = PiCamera() #create camera object #---------------------------- #Initialize experiment #---------------------------- camera.start_preview(rotation = 180, fullscreen = False, window = (0,-44,350,400)) #Set the time for the beginning of the block
GPIO.setmode(GPIO.BCM) #set the enable pins for L and R stepper motors to 1 to prevent overheating GPIO.setup(L_enablePIN, GPIO.OUT, initial = 1) GPIO.setup(R_enablePIN, GPIO.OUT, initial = 1) #create Stepper class instances for left and right reward delivery water_L = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN) water_R = core.stepper(R_enablePIN, R_directionPIN, R_stepPIN, R_emptyPIN) #create lickometer class instances for left and right lickometers lick_port_L = core.lickometer(L_lickometer) lick_port_R = core.lickometer(R_lickometer) #create tones tone_L = core.tones(L_tone_freq, 1) tone_R = core.tones(R_tone_freq, 1) tone_go = core.tones(go_tone_freq, 0.75) camera = PiCamera() #---------------------------- #Initialize experiment #---------------------------- camera.start_preview(rotation = 180, fullscreen = False, window = (0,-44,350,400)) #Set the time for the beginning of the block trials = np.arange(n_trials) data = core.data(n_trials, mouse_number, block_number)
#The thread will run the stepper.Refill method, and can run several instances simultaneously
left_thread.start() #start thread for L syringe
right_thread.start() #start thread for R syringe
tube_fill = input('Reconnect and fill water tubes (ENTER) ')
#reminder to reconnect and manually spin the syringe pumps
#----------------------------
#Test speakers:
#----------------------------
#initialize the mixer (for tones) at the proper sampling rate.
mixer.init(frequency=44100)
test_tone = core.tones(
1000, 2, 2) #generates a .wav file with a 2 second tone at 1000Hz
print('Testing speakers.')
speaker_test = 'a' #Initialize speaker_test variable to 'a'; see below
while speaker_test == 'a':
test_tone.Play() #play the test tone
speaker_test = input('Did you hear the tone? y:yes, a:again: ')
test_tone.Delete() #delete the .wav file that was created
#----------------------------
#Test lick detection:
#----------------------------
#Set the mode of the pins (broadcom vs local)
GPIO.setmode(GPIO.BCM)
#set the enable pins for L and R stepper motors to 1 to prevent overheating
GPIO.setup(L_enablePIN, GPIO.OUT, initial=1)
GPIO.setup(R_enablePIN, GPIO.OUT, initial=1)
#initialize the mixer (for tones) at the proper sampling rate.
mixer.init(frequency=44100)
#create Stepper class instance
water = core.stepper(L_enablePIN, L_directionPIN, L_stepPIN, L_emptyPIN)
#create tone
tone = core.tones(tone_freq, sample_tone_length,
single_pulse_length) #1000Hz single pulse
loop = True
while loop == True:
raw_input = input('Ready to play tone (ENTER) ')
tone.Play()
water.Reward()
time.sleep(2)
water.Reward()
tone.play()