def finish(self):
tm_start = time.ctime()
all_species = self.targets | self.outgroups
cogs, cog_analysis = brh_cogs2(db,
all_species,
missing_factor=self.missing_factor,
seed_sp=self.seed)
self.raw_cogs = cogs
self.cog_analysis = cog_analysis
self.cogs = []
for co in cogs:
# self.cogs.append(map(encode_seqname, co))
encoded_names = db.translate_names(co)
if len(encoded_names) != len(co):
print set(co) - set(encoded_names.keys())
raise DataError("Some sequence ids could not be translated")
self.cogs.append(encoded_names.values())
# Sort Cogs according to the md5 hash of its content. Random
# sorting but kept among runs
map(lambda x: x.sort(), self.cogs)
self.cogs.sort(lambda x, y: cmp(md5(','.join(x)), md5(','.join(y))))
log.log(28, "%s COGs detected" % len(self.cogs))
tm_end = time.ctime()
#open(pjoin(self.taskdir, "__time__"), "w").write(
# '\n'.join([tm_start, tm_end]))
CogSelectorTask.store_data(self, self.cogs, self.cog_analysis)
def finish(self):
tm_start = time.ctime()
all_species = self.targets | self.outgroups
cogs, cog_analysis = brh_cogs2(db, all_species,
missing_factor=self.missing_factor,
seed_sp=self.seed)
self.raw_cogs = cogs
self.cog_analysis = cog_analysis
self.cogs = []
for co in cogs:
# self.cogs.append(map(encode_seqname, co))
encoded_names = db.translate_names(co)
if len(encoded_names) != len(co):
print set(co) - set(encoded_names.keys())
raise DataError("Some sequence ids could not be translated")
self.cogs.append(encoded_names.values())
# Sort Cogs according to the md5 hash of its content. Random
# sorting but kept among runs
map(lambda x: x.sort(), self.cogs)
self.cogs.sort(lambda x,y: cmp(md5(','.join(x)), md5(','.join(y))))
log.log(28, "%s COGs detected" %len(self.cogs))
tm_end = time.ctime()
#open(pjoin(self.taskdir, "__time__"), "w").write(
# '\n'.join([tm_start, tm_end]))
CogSelectorTask.store_data(self, self.cogs, self.cog_analysis)
def finish(self):
def sort_cogs_by_size(c1, c2):
'''
sort cogs by descending size. If two cogs are the same size, sort
them keeping first the one with the less represented
species. Otherwise sort by sequence name sp_seqid.'''
r = -1 * cmp(len(c1), len(c2))
if r == 0:
# finds the cog including the less represented species
c1_repr = _min([sp2cogs[_sp] for _sp, _seq in c1])
c2_repr = _min([sp2cogs[_sp] for _sp, _seq in c2])
r = cmp(c1_repr, c2_repr)
if r == 0:
return cmp(sorted(c1), sorted(c2))
else:
return r
else:
return r
def sort_cogs_by_sp_repr(c1, c2):
c1_repr = _min([sp2cogs[_sp] for _sp, _seq in c1])
c2_repr = _min([sp2cogs[_sp] for _sp, _seq in c2])
r = cmp(c1_repr, c2_repr)
if r == 0:
r = -1 * cmp(len(c1), len(c2))
if r == 0:
return cmp(sorted(c1), sorted(c2))
else:
return r
else:
return r
all_species = self.targets | self.outgroups
# strict threshold
#min_species = len(all_species) - int(round(self.missing_factor * len(all_species)))
# Relax threshold for cog selection to ensure sames genes are always included
min_species = len(all_species) - int(round(self.missing_factor * len(GLOBALS["target_species"])))
min_species = max(min_species, (1-self.max_missing_factor) * len(all_species))
smallest_cog, largest_cog = len(all_species), 0
all_singletons = []
sp2cogs = defaultdict(int)
for cognumber, cog in enumerate(open(GLOBALS["cogs_file"])):
sp2seqs = defaultdict(list)
for sp, seqid in [map(strip, seq.split(GLOBALS["spname_delimiter"], 1)) for seq in cog.split("\t")]:
sp2seqs[sp].append(seqid)
one2one_cog = set()
for sp, seqs in sp2seqs.iteritems():
#if len(seqs) != 1:
# print sp, len(seqs)
if sp in all_species and len(seqs) == 1:
sp2cogs[sp] += 1
one2one_cog.add((sp, seqs[0]))
smallest_cog = min(smallest_cog, len(one2one_cog))
largest_cog = max(largest_cog, len(one2one_cog))
all_singletons.append(one2one_cog)
#if len(one2one_cog) >= min_species:
# valid_cogs.append(one2one_cog)
cognumber += 1 # sets the ammount of cogs in file
for sp, ncogs in sorted(sp2cogs.items(), key=lambda x: x[1], reverse=True):
log.log(28, "% 20s found in single copy in % 6d (%0.1f%%) COGs " %(sp, ncogs, 100 * ncogs/float(cognumber)))
valid_cogs = sorted([sing for sing in all_singletons if len(sing) >= min_species],
sort_cogs_by_size)
log.log(28, "Largest cog size: %s. Smallest cog size: %s" %(
largest_cog, smallest_cog))
self.cog_analysis = ""
# save original cog names hitting the hard limit
if len(valid_cogs) > self.cog_hard_limit:
log.warning("Applying hard limit number of COGs: %d out of %d available" %(self.cog_hard_limit, len(valid_cogs)))
self.raw_cogs = valid_cogs[:self.cog_hard_limit]
self.cogs = []
# Translate sequence names into the internal DB names
sp_repr = defaultdict(int)
sizes = []
for co in self.raw_cogs:
sizes.append(len(co))
for sp, seq in co:
sp_repr[sp] += 1
co_names = ["%s%s%s" %(sp, GLOBALS["spname_delimiter"], seq) for sp, seq in co]
encoded_names = db.translate_names(co_names)
if len(encoded_names) != len(co):
print set(co) - set(encoded_names.keys())
raise DataError("Some sequence ids could not be translated")
self.cogs.append(encoded_names.values())
# ERROR! COGs selected are not the prioritary cogs sorted out before!!!
# Sort Cogs according to the md5 hash of its content. Random
# sorting but kept among runs
#map(lambda x: x.sort(), self.cogs)
#self.cogs.sort(lambda x,y: cmp(md5(','.join(x)), md5(','.join(y))))
log.log(28, "Analysis of current COG selection:")
for sp, ncogs in sorted(sp_repr.items(), key=lambda x:x[1], reverse=True):
log.log(28, " % 30s species present in % 6d COGs (%0.1f%%)" %(sp, ncogs, 100 * ncogs/float(len(self.cogs))))
log.log(28, " %d COGs selected with at least %d species out of %d" %(len(self.cogs), min_species, len(all_species)))
log.log(28, " Average COG size %0.1f/%0.1f +- %0.1f" %(_mean(sizes), _median(sizes), _std(sizes)))
# Some consistency checks
missing_sp = (all_species) - set(sp_repr.keys())
if missing_sp:
log.error("%d missing species or not present in single-copy in any cog:\n%s" %\
(len(missing_sp), '\n'.join(missing_sp)))
open('etebuild.valid_species_names.tmp', 'w').write('\n'.join(sp_repr.keys()) +'\n')
log.error("All %d valid species have been dumped into etebuild.valid_species_names.tmp."
" You can use --spfile to restrict the analysis to those species." %len(sp_repr))
raise TaskError('missing or not single-copy species under current cog selection')
CogSelectorTask.store_data(self, self.cogs, self.cog_analysis)