def main(args):
hl.init(log='/liftover.log')
if args.gnomad:
gnomad = True
path = None
if args.exomes:
data_type = 'exomes'
if args.genomes:
data_type = 'genomes'
logger.info('Working on gnomAD {} release ht'.format(data_type))
logger.info('Reading in release ht')
t = public_release(data_type).ht()
logger.info('Variants in release ht: {}'.format(t.count()))
else:
data_type = None
gnomad = False
if args.ht:
path = args.ht
t = hl.read_table(args.ht)
if args.mt:
path = args.mt
t = hl.read_matrix_table(args.mt)
logger.info('Checking if input data has been split')
if 'was_split' not in t.row:
t = hl.split_multi(t) if isinstance(
t, hl.Table) else hl.split_multi_hts(t)
logger.info('Preparing reference genomes for liftover')
source, target = get_liftover_genome(t)
if args.test:
logger.info('Filtering to chr21 for testing')
if source.name == 'GRCh38':
contig = 'chr21'
else:
contig = '21'
t = hl.filter_intervals(
t, [hl.parse_locus_interval(contig, reference_genome=source.name)])
logger.info(f'Lifting data to {target.name}')
t = lift_data(t, gnomad, data_type, path, target, args.overwrite)
logger.info('Checking SNPs for reference mismatches')
t = annotate_snp_mismatch(t, data_type, target)
mismatch = check_mismatch(t) if isinstance(
t, hl.Table) else check_mismatch(t.rows())
logger.info('{} total SNPs'.format(mismatch['total_variants']))
logger.info('{} SNPs on minus strand'.format(mismatch['negative_strand']))
logger.info('{} reference mismatches in SNPs'.format(
mismatch['total_mismatch']))
logger.info('{} mismatches on minus strand'.format(
mismatch['negative_strand_mismatch']))
def prepare_gnomad_stats(overwrite):
ht = hl.read_table(get_ukb_vep_path())
gwased_variants_ht = hl.read_matrix_table(
get_variant_results_path('full')).rows()
ht = ht.filter(hl.is_defined(gwased_variants_ht[ht.key]))
datasets = ('exomes', 'genomes')
gnomad_hts = {
dataset: gnomad.public_release(dataset).ht()
for dataset in datasets
}
gnomad_index_dicts = {
k: gnomad_ht.index_globals().freq_index_dict
for k, gnomad_ht in gnomad_hts.items()
}
gnomads = {k: gnomad_ht[ht.key] for k, gnomad_ht in gnomad_hts.items()}
ht = ht.annotate(freq=[
hl.struct(pop=pop,
ac=ht.af[pop] * ht.an[pop],
af=ht.af[pop] / 2,
an=ht.an[pop] * 2,
**{
f'gnomad_{dataset}_{metric.lower()}':
gnomads[dataset].freq[gnomad_index_dicts[dataset].get(
f'gnomad_{UKB_GNOMAD_POP_MAPPING[pop]}')][metric]
for dataset in datasets for metric in ('AC', 'AF', 'AN')
}) for pop in POPS
],
**{
f'pass_gnomad_{dataset}':
hl.len(gnomads[dataset].filters) == 0
for dataset in datasets
})
ht.naive_coalesce(1000).write(get_analysis_data_path(
'gnomad_comparison', 'qc', 'full', 'ht'),
overwrite=overwrite)
def get_r_within_gene(
bm: BlockMatrix,
ld_index: hl.Table,
gene: str,
vep_ht: hl.Table = None,
reference_genome: str = None,
):
"""
Get LD information (`r`) for all pairs of variants within `gene`.
Warning: this returns a table quadratic in number of variants. Exercise caution with large genes.
:param bm: Input Block Matrix
:param ld_index: Corresponding index table
:param gene: Gene symbol as string
:param vep_ht: Table with VEP annotations (if None, gets from get_gnomad_public_data())
:param reference_genome: Reference genome to pass to get_gene_intervals for fast filtering to gene
:return: Table with pairs of variants
"""
if vep_ht is None:
vep_ht = public_release("exomes").ht()
if reference_genome is None:
reference_genome = hl.default_reference().name
intervals = hl.experimental.get_gene_intervals(
gene_symbols=[gene], reference_genome=reference_genome)
ld_index = hl.filter_intervals(ld_index, intervals)
ld_index = ld_index.annotate(vep=vep_ht[ld_index.key].vep)
ld_index = ld_index.filter(
hl.any(lambda tc: tc.gene_symbol == gene,
ld_index.vep.transcript_consequences))
indices_to_keep = ld_index.idx.collect()
filt_bm = bm.filter(indices_to_keep, indices_to_keep)
ht = filt_bm.entries()
ld_index = ld_index.add_index("new_idx").key_by("new_idx")
return ht.transmute(r=ht.entry,
i_variant=ld_index[ht.i],
j_variant=ld_index[ht.j])
def annotate_unphased_pairs(unphased_ht: hl.Table, n_variant_pairs: int,
least_consequence: str, max_af: float):
# unphased_ht = vp_ht.filter(hl.is_missing(vp_ht.all_phase))
# unphased_ht = unphased_ht.key_by()
# Explode variant pairs
unphased_ht = unphased_ht.annotate(las=[
hl.tuple([unphased_ht.locus1, unphased_ht.alleles1]),
hl.tuple([unphased_ht.locus2, unphased_ht.alleles2])
]).explode('las', name='la')
unphased_ht = unphased_ht.key_by(
locus=unphased_ht.la[0], alleles=unphased_ht.la[1]).persist(
) # .checkpoint('gs://gnomad-tmp/vp_ht_unphased.ht')
# Annotate single variants with gnomAD freq
gnomad_ht = gnomad.public_release('exomes').ht()
gnomad_ht = gnomad_ht.semi_join(unphased_ht).repartition(
ceil(n_variant_pairs / 10000), shuffle=True).persist()
missing_freq = hl.struct(
AC=0,
AF=0,
AN=125748 * 2, # set to no missing for now
homozygote_count=0)
logger.info(
f"{gnomad_ht.count()}/{unphased_ht.count()} single variants from the unphased pairs found in gnomAD."
)
gnomad_indexed = gnomad_ht[unphased_ht.key]
gnomad_freq = gnomad_indexed.freq
unphased_ht = unphased_ht.annotate(
adj_freq=hl.or_else(gnomad_freq[0], missing_freq),
raw_freq=hl.or_else(gnomad_freq[1], missing_freq),
vep_genes=vep_genes_expr(gnomad_indexed.vep, least_consequence),
max_af_filter=gnomad_indexed.freq[0].AF <= max_af
# pop_max_freq=hl.or_else(
# gnomad_exomes.popmax[0],
# missing_freq.annotate(
# pop=hl.null(hl.tstr)
# )
# )
)
unphased_ht = unphased_ht.persist()
# unphased_ht = unphased_ht.checkpoint('gs://gnomad-tmp/unphased_ann.ht', overwrite=True)
loci_expr = hl.sorted(
hl.agg.collect(
hl.tuple([
unphased_ht.locus,
hl.struct(
adj_freq=unphased_ht.adj_freq,
raw_freq=unphased_ht.raw_freq,
# pop_max_freq=unphased_ht.pop_max_freq
)
])),
lambda x: x[0] # sort by locus
).map(lambda x: x[1] # get rid of locus
)
vp_freq_expr = hl.struct(v1=loci_expr[0], v2=loci_expr[1])
# [AABB, AABb, AAbb, AaBB, AaBb, Aabb, aaBB, aaBb, aabb]
def get_gt_counts(freq: str):
return hl.array([
hl.min(vp_freq_expr.v1[freq].AN, vp_freq_expr.v2[freq].AN), # AABB
vp_freq_expr.v2[freq].AC -
(2 * vp_freq_expr.v2[freq].homozygote_count), # AABb
vp_freq_expr.v2[freq].homozygote_count, # AAbb
vp_freq_expr.v1[freq].AC -
(2 * vp_freq_expr.v1[freq].homozygote_count), # AaBB
0, # AaBb
0, # Aabb
vp_freq_expr.v1[freq].homozygote_count, # aaBB
0, # aaBb
0 # aabb
])
gt_counts_raw_expr = get_gt_counts('raw_freq')
gt_counts_adj_expr = get_gt_counts('adj_freq')
# gt_counts_pop_max_expr = get_gt_counts('pop_max_freq')
unphased_ht = unphased_ht.group_by(
unphased_ht.locus1, unphased_ht.alleles1, unphased_ht.locus2,
unphased_ht.alleles2
).aggregate(
pop='all', # TODO Add option for multiple pops?
phase_info=hl.struct(gt_counts=hl.struct(raw=gt_counts_raw_expr,
adj=gt_counts_adj_expr),
em=hl.struct(
raw=get_em_expr(gt_counts_raw_expr),
adj=get_em_expr(gt_counts_raw_expr))),
vep_genes=hl.agg.collect(
unphased_ht.vep_genes).filter(lambda x: hl.len(x) > 0),
max_af_filter=hl.agg.all(unphased_ht.max_af_filter)
# pop_max_gt_counts_adj=gt_counts_raw_expr,
# pop_max_em_p_chet_adj=get_em_expr(gt_counts_raw_expr).p_chet,
) # .key_by()
unphased_ht = unphased_ht.transmute(
vep_filter=(hl.len(unphased_ht.vep_genes) > 1)
& (hl.len(unphased_ht.vep_genes[0].intersection(
unphased_ht.vep_genes[1])) > 0))
max_af_filtered, vep_filtered = unphased_ht.aggregate([
hl.agg.count_where(~unphased_ht.max_af_filter),
hl.agg.count_where(~unphased_ht.vep_filter)
])
if max_af_filtered > 0:
logger.info(
f"{max_af_filtered} variant-pairs excluded because the AF of at least one variant was > {max_af}"
)
if vep_filtered > 0:
logger.info(
f"{vep_filtered} variant-pairs excluded because the variants were not found within the same gene with a csq of at least {least_consequence}"
)
unphased_ht = unphased_ht.filter(unphased_ht.max_af_filter
& unphased_ht.vep_filter)
return unphased_ht.drop('max_af_filter', 'vep_filter')
