def nucmer(args):
"""
%prog nucmer ref.fasta query.fasta
Run NUCMER using query against reference. Parallel implementation derived
from: <https://github.com/fritzsedlazeck/sge_mummer>
"""
from itertools import product
from jcvi.apps.grid import MakeManager
from jcvi.formats.base import split
p = OptionParser(nucmer.__doc__)
p.add_option("--chunks",
type="int",
help="Split both query and subject into chunks")
p.set_params(prog="nucmer", params="-g 5000 -l 24 -c 500")
p.set_cpus()
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
ref, query = args
cpus = opts.cpus
nrefs = nqueries = opts.chunks or int(cpus**.5)
refdir = ref.split(".")[0] + "-outdir"
querydir = query.split(".")[0] + "-outdir"
reflist = split([ref, refdir, str(nrefs)]).names
querylist = split([query, querydir, str(nqueries)]).names
mm = MakeManager()
for i, (r, q) in enumerate(product(reflist, querylist)):
pf = "{0:04d}".format(i)
cmd = "nucmer -maxmatch"
cmd += " {0}".format(opts.extra)
cmd += " {0} {1} -p {2}".format(r, q, pf)
deltafile = pf + ".delta"
mm.add((r, q), deltafile, cmd)
print cmd
mm.write()
def nucmer(args):
"""
%prog nucmer ref.fasta query.fasta
Run NUCMER using query against reference. Parallel implementation derived
from: <https://github.com/fritzsedlazeck/sge_mummer>
"""
from itertools import product
from jcvi.apps.grid import MakeManager
from jcvi.formats.base import split
p = OptionParser(nucmer.__doc__)
p.add_option("--chunks", type="int",
help="Split both query and subject into chunks")
p.set_params(prog="nucmer", params="-l 100 -c 500")
p.set_cpus()
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
ref, query = args
cpus = opts.cpus
nrefs = nqueries = opts.chunks or int(cpus ** .5)
refdir = ref.split(".")[0] + "-outdir"
querydir = query.split(".")[0] + "-outdir"
reflist = split([ref, refdir, str(nrefs)]).names
querylist = split([query, querydir, str(nqueries)]).names
mm = MakeManager()
for i, (r, q) in enumerate(product(reflist, querylist)):
pf = "{0:04d}".format(i)
cmd = "nucmer -maxmatch"
cmd += " {0}".format(opts.extra)
cmd += " {0} {1} -p {2}".format(r, q, pf)
deltafile = pf + ".delta"
mm.add((r, q), deltafile, cmd)
print cmd
mm.write()
def augustus(args):
"""
%prog augustus fastafile
Run parallel AUGUSTUS. Final results can be reformatted using
annotation.reformat.augustus().
"""
p = OptionParser(augustus.__doc__)
p.add_option("--species",
default="maize",
help="Use species model for prediction")
p.add_option("--hintsfile", help="Hint-guided AUGUSTUS")
p.add_option("--nogff3",
default=False,
action="store_true",
help="Turn --gff3=off")
p.set_home("augustus")
p.set_cpus()
opts, args = p.parse_args(args)
if len(args) != 1:
sys.exit(not p.print_help())
(fastafile, ) = args
cpus = opts.cpus
mhome = opts.augustus_home
gff3 = not opts.nogff3
suffix = ".gff3" if gff3 else ".out"
cfgfile = op.join(mhome, "config/extrinsic/extrinsic.M.RM.E.W.cfg")
outdir = mkdtemp(dir=".")
fs = split([fastafile, outdir, str(cpus)])
augustuswrap_params = partial(
augustuswrap,
species=opts.species,
gff3=gff3,
cfgfile=cfgfile,
hintsfile=opts.hintsfile,
)
g = Jobs(augustuswrap_params, fs.names)
g.run()
gff3files = [x.rsplit(".", 1)[0] + suffix for x in fs.names]
outfile = fastafile.rsplit(".", 1)[0] + suffix
FileMerger(gff3files, outfile=outfile).merge()
shutil.rmtree(outdir)
if gff3:
from jcvi.annotation.reformat import augustus as reformat_augustus
reformat_outfile = outfile.replace(".gff3", ".reformat.gff3")
reformat_augustus([outfile, "--outfile={0}".format(reformat_outfile)])
def parallel_musclewrap(clustfile, cpus, minsamp=0):
musclewrap_minsamp = partial(musclewrap, minsamp=minsamp)
if cpus == 1:
return musclewrap_minsamp(clustfile)
from jcvi.apps.grid import Jobs
outdir = mkdtemp(dir=".")
fs = split([clustfile, outdir, str(cpus), "--format=clust"])
g = Jobs(musclewrap_minsamp, fs.names)
g.run()
clustnames = [x.replace(".clust", ".clustS") for x in fs.names]
clustSfile = clustfile.replace(".clust", ".clustS")
FileMerger(clustnames, outfile=clustSfile).merge()
shutil.rmtree(outdir)
def augustus(args):
"""
%prog augustus fastafile
Run parallel AUGUSTUS. Final results can be reformatted using
annotation.reformat.augustus().
"""
p = OptionParser(augustus.__doc__)
p.add_option("--species", default="maize",
help="Use species model for prediction")
p.add_option("--hintsfile", help="Hint-guided AUGUSTUS")
p.add_option("--nogff3", default=False, action="store_true",
help="Turn --gff3=off")
p.set_home("augustus")
p.set_cpus()
opts, args = p.parse_args(args)
if len(args) != 1:
sys.exit(not p.print_help())
fastafile, = args
cpus = opts.cpus
mhome = opts.augustus_home
gff3 = not opts.nogff3
suffix = ".gff3" if gff3 else ".out"
cfgfile = op.join(mhome, "config/extrinsic/extrinsic.M.RM.E.W.cfg")
outdir = mkdtemp(dir=".")
fs = split([fastafile, outdir, str(cpus)])
augustuswrap_params = partial(augustuswrap, species=opts.species,
gff3=gff3, cfgfile=cfgfile,
hintsfile=opts.hintsfile)
g = Jobs(augustuswrap_params, fs.names)
g.run()
gff3files = [x.rsplit(".", 1)[0] + suffix for x in fs.names]
outfile = fastafile.rsplit(".", 1)[0] + suffix
FileMerger(gff3files, outfile=outfile).merge()
shutil.rmtree(outdir)
if gff3:
from jcvi.annotation.reformat import augustus as reformat_augustus
reformat_outfile = outfile.replace(".gff3", ".reformat.gff3")
reformat_augustus([outfile, "--outfile={0}".format(reformat_outfile)])
def parallel(args):
"""
%prog parallel genome.fasta N
Partition the genome into parts and run separately. This is useful if MAKER
is to be run on the grid.
"""
from jcvi.formats.base import split
p = OptionParser(parallel.__doc__)
p.set_home("maker")
p.set_tmpdir(tmpdir="tmp")
p.set_grid_opts(array=True)
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
genome, NN = args
threaded = opts.threaded or 1
tmpdir = opts.tmpdir
mkdir(tmpdir)
tmpdir = get_abs_path(tmpdir)
N = int(NN)
assert 1 <= N < 1000, "Required: 1 < N < 1000!"
outdir = "outdir"
fs = split([genome, outdir, NN])
c = CTLFile("maker_opts.ctl")
c.update_abs_path()
if threaded > 1:
c.update_tag("cpus", threaded)
cwd = os.getcwd()
dirs = []
for name in fs.names:
fn = get_abs_path(name)
bn = op.basename(name)
dirs.append(bn)
c.update_tag("genome", fn)
mkdir(bn)
sh("cp *.ctl {0}".format(bn))
os.chdir(bn)
c.write_file("maker_opts.ctl")
os.chdir(cwd)
jobs = "jobs"
fw = open(jobs, "w")
print("\n".join(dirs), file=fw)
fw.close()
# Submit to grid
ncmds = len(dirs)
runfile = "array.sh"
cmd = op.join(opts.maker_home, "bin/maker")
if tmpdir:
cmd += " -TMP {0}".format(tmpdir)
engine = get_grid_engine()
contents = arraysh.format(jobs, cmd) if engine == "SGE" \
else arraysh_ua.format(N, threaded, jobs, cmd)
write_file(runfile, contents)
if engine == "PBS":
return
# qsub script
outfile = "maker.\$TASK_ID.out"
p = GridProcess(runfile,
outfile=outfile,
errfile=outfile,
arr=ncmds,
grid_opts=opts)
qsubfile = "qsub.sh"
qsub = p.build()
write_file(qsubfile, qsub)
def parallel(args):
"""
%prog parallel genome.fasta N
Partition the genome into parts and run separately. This is useful if MAKER
is to be run on the grid.
"""
from jcvi.formats.base import split
p = OptionParser(parallel.__doc__)
p.set_home("maker")
p.set_tmpdir(tmpdir="tmp")
p.set_grid_opts(array=True)
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
genome, NN = args
threaded = opts.threaded or 1
tmpdir = opts.tmpdir
mkdir(tmpdir)
tmpdir = get_abs_path(tmpdir)
N = int(NN)
assert 1 <= N < 1000, "Required: 1 < N < 1000!"
outdir = "outdir"
fs = split([genome, outdir, NN])
c = CTLFile("maker_opts.ctl")
c.update_abs_path()
if threaded > 1:
c.update_tag("cpus", threaded)
cwd = os.getcwd()
dirs = []
for name in fs.names:
fn = get_abs_path(name)
bn = op.basename(name)
dirs.append(bn)
c.update_tag("genome", fn)
mkdir(bn)
sh("cp *.ctl {0}".format(bn))
os.chdir(bn)
c.write_file("maker_opts.ctl")
os.chdir(cwd)
jobs = "jobs"
fw = open(jobs, "w")
print("\n".join(dirs), file=fw)
fw.close()
# Submit to grid
ncmds = len(dirs)
runfile = "array.sh"
cmd = op.join(opts.maker_home, "bin/maker")
if tmpdir:
cmd += " -TMP {0}".format(tmpdir)
engine = get_grid_engine()
contents = arraysh.format(jobs, cmd) if engine == "SGE" \
else arraysh_ua.format(N, threaded, jobs, cmd)
write_file(runfile, contents)
if engine == "PBS":
return
# qsub script
outfile = "maker.\$TASK_ID.out"
p = GridProcess(runfile, outfile=outfile, errfile=outfile,
arr=ncmds, grid_opts=opts)
qsubfile = "qsub.sh"
qsub = p.build()
write_file(qsubfile, qsub)
def main():
"""
%prog database.fa query.fa [options]
Wrapper for NCBI BLAST+.
"""
p = OptionParser(main.__doc__)
p.add_option("--format", default=" \'6 qseqid sseqid pident length " \
"mismatch gapopen qstart qend sstart send evalue bitscore\' ",
help="0-11, learn more with \"blastp -help\". [default: %default]")
p.add_option("--path", dest="blast_path", default=None,
help="specify BLAST+ path including the program name")
p.add_option("--prog", dest="blast_program", default="blastp",
help="specify BLAST+ program to use. See complete list here: " \
"http://www.ncbi.nlm.nih.gov/books/NBK52640/#chapter1.Installation"
" [default: %default]")
p.set_align(evalue=.01)
p.add_option("--best", default=1, type="int",
help="Only look for best N hits [default: %default]")
p.set_cpus()
p.add_option("--nprocs", default=1, type="int",
help="number of BLAST processes to run in parallel. " + \
"split query.fa into `nprocs` chunks, " + \
"each chunk uses -num_threads=`cpus`")
p.set_params()
p.set_outfile()
opts, args = p.parse_args()
if len(args) != 2 or opts.blast_program is None:
sys.exit(not p.print_help())
bfasta_fn, afasta_fn = args
for fn in (afasta_fn, bfasta_fn):
assert op.exists(fn)
afasta_fn = op.abspath(afasta_fn)
bfasta_fn = op.abspath(bfasta_fn)
out_fh = must_open(opts.outfile, "w")
extra = opts.extra
blast_path = opts.blast_path
blast_program = opts.blast_program
blast_bin = blast_path or blast_program
if op.basename(blast_bin) != blast_program:
blast_bin = op.join(blast_bin, blast_program)
nprocs, cpus = opts.nprocs, opts.cpus
if nprocs > 1:
logging.debug("Dispatch job to %d processes" % nprocs)
outdir = "outdir"
fs = split([afasta_fn, outdir, str(nprocs)])
queries = fs.names
else:
queries = [afasta_fn]
dbtype = "prot" if op.basename(blast_bin) in ("blastp", "blastx") \
else "nucl"
db = bfasta_fn
if dbtype == "prot":
nin = db + ".pin"
else:
nin = db + ".nin"
nin00 = db + ".00.nin"
nin = nin00 if op.exists(nin00) else (db + ".nin")
run_formatdb(infile=db, outfile=nin, dbtype=dbtype)
lock = Lock()
blastplus_template = "{0} -db {1} -outfmt {2}"
blast_cmd = blastplus_template.format(blast_bin, bfasta_fn, opts.format)
blast_cmd += " -evalue {0} -max_target_seqs {1}".\
format(opts.evalue, opts.best)
blast_cmd += " -num_threads {0}".format(cpus)
if extra:
blast_cmd += " " + extra.strip()
args = [(out_fh, blast_cmd, query, lock) for query in queries]
g = Jobs(target=blastplus, args=args)
g.run()
