Add named or unnamed vispy colormaps to existing layers.
"""
import numpy as np
import vispy.color
from skimage import data
import napari
histo = data.astronaut() / 255
rch, gch, bch = np.transpose(histo, (2, 0, 1))
red = vispy.color.Colormap([[0.0, 0.0, 0.0], [1.0, 0.0, 0.0]])
green = vispy.color.Colormap([[0.0, 0.0, 0.0], [0.0, 1.0, 0.0]])
blue = vispy.color.Colormap([[0.0, 0.0, 0.0], [0.0, 0.0, 1.0]])
v = napari.Viewer()
rlayer = v.add_image(rch, name='red channel')
rlayer.blending = 'additive'
rlayer.colormap = 'red', red
glayer = v.add_image(gch, name='green channel')
glayer.blending = 'additive'
glayer.colormap = green # this will appear as [unnamed colormap]
blayer = v.add_image(bch, name='blue channel')
blayer.blending = 'additive'
blayer.colormap = {'blue': blue}
if __name__ == '__main__':
napari.run()
def view_plate(
image_data,
label_data=None,
image_settings=None,
label_settings=None,
well_measurements=None,
well_shape=None,
zero_based=True,
well_spacing=16,
site_spacing=4,
show=True,
):
"""Visualize data from a multi-well plate using napari.
Args:
image_data dict[str, [dict[str, list[np.array]]]]: list of image sources,
each list contains a dict which maps the well names (e.g. A1, B3) to
the image data for this well (one array per well position)
label_data dict[str, [dict[str, list[np.array]]]]: list of label sources,
each list contains a dict which maps the well names (e.g. A1, B3) to
the label data for this well (one array per well position) (default: None)
image_settings dict[str, dict]: image settings for the channels (default: None)
label_settings dict[str, dict]: settings for the label channels (default: None)
well_measurements dict[str, dict[str, [float, int, str]]]: measurements associated with the wells
well_shape tuple[int]: the 2D shape of a well in terms of images, if not given will be derived.
Well shape can only be derived for square wells and must be passed otherwise (default: None)
zero_based bool: whether the well indexing is zero-based (default: True)
well_sources int: spacing between wells, in pixels (default: 12)
site_spacing int: spacing between sites, in pixels (default: 4)
show bool: whether to show the viewer (default: True)
"""
# find the number of positions per well
first_channel_sources = next(iter(image_data.values()))
pos_per_well = len(next(iter(first_channel_sources.values())))
# find the well shape
if well_shape is None: # well shape can only be derived for square wells
assert pos_per_well in (1, 4, 9, 25, 36,
49), f"well is not square: {pos_per_well}"
well_len = int(np.sqrt(pos_per_well))
well_shape = (well_len, well_len)
else:
assert len(well_shape) == 2
pos_per_well_exp = np.prod(list(well_shape))
assert pos_per_well_exp == pos_per_well, f"{pos_per_well_exp} != {pos_per_well}"
def process_sources(sources, well_names):
for well_sources in sources.values():
# make sure all wells have the same number of label
n_pos_well = [len(sources) for sources in well_sources.values()]
assert all(
n_pos == pos_per_well
for n_pos in n_pos_well), f"{pos_per_well} != {n_pos_well}"
well_names.extend(list(well_sources.keys()))
return well_names
# find the well names for all sources
well_names = process_sources(image_data, [])
if label_data is not None:
well_names = process_sources(label_data, well_names)
well_names = list(set(well_names))
well_names.sort()
# compute the well extent and well positions
well_positions, well_start, well_stop = parse_wells(well_names, zero_based)
assert len(well_positions) == len(well_names)
# start the veiwer and add all sources
viewer = napari.Viewer()
shape = add_grid_sources(image_data, well_positions, well_shape,
well_spacing, site_spacing, viewer.add_image,
image_settings)
if label_data is not None:
add_grid_sources(label_data, well_positions, well_shape, well_spacing,
site_spacing, viewer.add_labels, label_settings)
# add shape layer corresponding to the well positions
add_plate_layout(viewer,
well_names,
well_positions,
well_shape,
well_spacing,
site_spacing,
shape,
measurements=well_measurements)
# set the camera so that the initial view is centered around the existing wells
# and zoom out so that the central well is fully visible
set_camera(viewer, well_start, well_stop, well_shape, well_spacing,
site_spacing, shape)
if show:
napari.run()
return viewer
def run_cli(args):
LI = calciumcharacterisation.LazyImarisTSReader(args.imarisfile[0])
v = napari.Viewer()
l = v.add_image(LI.daskseries(), multiscale=False, name=args.imarisfile[0])
napari.run()
def _run():
from napari import run, view_path
from napari.settings import get_settings
"""Main program."""
args, kwargs = parse_sys_argv()
# parse -v flags and set the appropriate logging level
levels = [logging.WARNING, logging.INFO, logging.DEBUG]
level = levels[min(2, args.verbose)] # prevent index error
logging.basicConfig(
level=level,
format="%(asctime)s %(levelname)s %(message)s",
datefmt='%H:%M:%S',
)
if args.reset:
if args.settings_path:
settings = get_settings(path=args.settings_path)
else:
settings = get_settings()
settings.reset()
settings.save()
sys.exit("Resetting settings to default values.\n")
if args.plugin:
# make sure plugin is only used when files are specified
if not args.paths:
sys.exit(
"error: The '--plugin' argument is only valid "
"when providing a file name"
)
# I *think* that Qt is looking in sys.argv for a flag `--plugins`,
# which emits "WARNING: No such plugin for spec 'builtins'"
# so remove --plugin from sys.argv to prevent that warningz
sys.argv.remove('--plugin')
if any(p.endswith('.py') for p in args.paths):
# we're running a script
if len(args.paths) > 1:
sys.exit(
'When providing a python script, only a '
'single positional argument may be provided'
)
# run the file
mod = runpy.run_path(args.paths[0])
from napari_plugin_engine.markers import HookImplementationMarker
# if this file had any hook implementations, register and run as plugin
if any(isinstance(i, HookImplementationMarker) for i in mod.values()):
_run_plugin_module(mod, os.path.basename(args.paths[0]))
else:
if args.with_:
from .plugins import plugin_manager
# if a plugin widget has been requested, this will fail immediately
# if the requested plugin/widget is not available.
plugin_manager.discover_widgets()
pname, *wnames = args.with_
if wnames:
for wname in wnames:
_npe2.get_widget_contribution(
pname, wname
) or plugin_manager.get_widget(pname, wname)
else:
_npe2.get_widget_contribution(
pname
) or plugin_manager.get_widget(pname)
from napari._qt.widgets.qt_splash_screen import NapariSplashScreen
splash = NapariSplashScreen()
splash.close() # will close once event loop starts
# viewer is unused but _must_ be kept around.
# it will be referenced by the global window only
# once napari has finished starting
# but in the meantime if the garbage collector runs;
# it will collect it and hang napari at start time.
# in a way that is machine, os, time (and likely weather dependant).
viewer = view_path( # noqa: F841
args.paths,
stack=args.stack,
plugin=args.plugin,
layer_type=args.layer_type,
**kwargs,
)
if args.with_:
pname, *wnames = args.with_
if wnames:
for wname in wnames:
viewer.window.add_plugin_dock_widget(pname, wname)
else:
viewer.window.add_plugin_dock_widget(pname)
# only necessary in bundled app, but see #3596
from napari.utils.misc import (
install_certifi_opener,
running_as_bundled_app,
)
if running_as_bundled_app:
install_certifi_opener()
run(gui_exceptions=True)