def aspirate_from_well(well, volume, z_change=0): positions = [[0.5, "x"], [-0.5, "x"], [0.5, "y"], [-0.5, "y"]] volume = int(volume / 5) for it in range(5): if it == 0: pipette_300.aspirate(volume, ax_6.wells(well).top(z_change)) pipette_300.delay(seconds=2) speed_set = { 'x': 100, 'y': 100, 'z': 20, 'a': 20, 'b': 10, 'c': 10 } robot.head_speed(combined_speed=max(speed_set.values()), **speed_set) else: pipette_300.move_to(ax_6.wells(well).top(z_change)) calibration_functions.jog_instrument(instrument=pipette_300, distance=positions[it - 1][0], axis=positions[it - 1][1], robot=robot) pipette_300.aspirate(volume) pipette_300.delay(seconds=1) speed_set = {'x': 300, 'y': 200, 'z': 75, 'a': 75, 'b': 25, 'c': 25} robot.head_speed(combined_speed=max(speed_set.values()), **speed_set)
def jog(self, instrument, distance, axis): inst = instrument._instrument log.info('Jogging {} by {} in {}'.format(instrument.name, distance, axis)) self._set_state('moving') calibration_functions.jog_instrument(instrument=inst, distance=distance, axis=axis, robot=inst.robot) self._set_state('ready')
def jog(self, instrument, distance, axis): inst = instrument._instrument log.info('Jogging {} by {} in {}'.format(instrument.name, distance, axis)) self._set_state('moving') if ff.use_protocol_api_v2(): self._hardware.move_rel(Mount[inst.mount.upper()], Point(**{axis: distance})) else: calibration_functions.jog_instrument(instrument=inst, distance=distance, axis=axis, robot=inst.robot) self._set_state('ready')
def jog(self, instrument, distance, axis): inst = instrument._instrument log.info('Jogging {} by {} in {}'.format(instrument.name, distance, axis)) self._set_state('moving') if instrument._context: try: self._hardware.move_rel(Mount[inst.mount.upper()], Point(**{axis: distance}), check_bounds=MotionChecks.HIGH) except OutOfBoundsMove: log.exception('Out of bounds jog') else: calibration_functions.jog_instrument(instrument=inst, distance=distance, axis=axis, robot=inst.robot) self._set_state('ready')
print(usetip(1)) if cal_offset == 1: pipette_300.move_to(ax_6.wells("A1").top(10)) print("with offset") else: pipette_300.move_to(ax_6.wells("A1").top()) while True: mov_dir = input("Select movement direction and step size: ") mov_dir = mov_dir.split() calibrate_end = bool(int(mov_dir[2])) if calibrate_end == True: break else: step_dir = str(mov_dir[0]) step_size = float(mov_dir[1]) calibration_functions.jog_instrument(instrument=pipette_300,distance=step_size,axis=step_dir,robot=robot) if step_dir == "x": offset[0] = offset[0] + step_size elif step_dir == "y": offset[1] = offset[1] + step_size elif step_dir == "z": offset[2] = offset[2] + step_size if int(mov_dir[2]) > 1: print("Not saving Calibration") print("newline") else: if cal_offset == 1: update_offset("ax6_5",False, offset[0], offset[1], offset[2]+10) print("with offset")