def _particlesToEmx(emxData, partSet, micSet=None, **kwargs):
""" Write a SetOfMicrograph as expected in EMX format
Params:
micSet: input set of micrographs
filename: the EMX file where to store the micrographs information.
micSet: micrographs set associated with the particles
**kwargs: writeImages: if set to False, only coordinates are exported.
imagesStack: if passed all images will be output into a single
stack file.
imagesPrefix: used when not imagesStack is passed. A different
stack will be created per micrograph.
"""
writeImages = kwargs.get('writeImages', True)
imagesPrefix = kwargs.get('imagesPrefix', None)
micDict = {}
# Use singleMic for count all particles to be written to a single stack
imagesStack = kwargs.get('imagesStack', None)
singleMic = Micrograph()
singleMic.setFileName(imagesStack)
singleMic.counter = pwobj.Integer(0)
def _getMicKey(particle):
coord = particle.getCoordinate()
if coord is None or coord.getMicName() is None:
return '%05d' % particle.getMicId()
else:
return pwutils.removeExt(coord.getMicName())
def _getLocation(particle):
if imagesStack is not None:
mic = singleMic
else:
micKey = _getMicKey(particle)
if micKey not in micDict:
mic = Micrograph()
mic.setFileName(join(imagesPrefix,
'particles_%s.mrc' % micKey))
mic.counter = pwobj.Integer(0)
micDict[micKey] = mic
else:
mic = micDict[micKey]
# Count one more particle assigned to this micrograph
mic.counter.increment()
return (mic.counter.get(), mic.getFileName())
ih = ImageHandler()
partAlign = partSet.getAlignment()
for particle in partSet:
if writeImages:
newLoc = _getLocation(particle)
ih.convert(particle, newLoc)
localFn = basename(newLoc[1])
particle.setLocation(newLoc[0], localFn)
emxObj = _particleToEmx(emxData, particle, micSet, partAlign)
else:
emxObj = _coordinateToEmx(emxData, particle, micSet)
emxData.addObject(emxObj)
def _particlesToEmx(emxData, partSet, micSet=None, **kwargs):
""" Write a SetOfMicrograph as expected in EMX format
Params:
micSet: input set of micrographs
filename: the EMX file where to store the micrographs information.
micSet: micrographs set associated with the particles
**kwargs: writeImages: if set to False, only coordinates are exported.
imagesStack: if passed all images will be output into a single
stack file.
imagesPrefix: used when not imagesStack is passed. A different
stack will be created per micrograph.
"""
writeImages = kwargs.get('writeImages', True)
imagesPrefix = kwargs.get('imagesPrefix', None)
micDict = {}
# Use singleMic for count all particles to be written to a single stack
imagesStack = kwargs.get('imagesStack', None)
singleMic = Micrograph()
singleMic.setFileName(imagesStack)
singleMic.counter = pwobj.Integer(0)
def _getMicKey(particle):
coord = particle.getCoordinate()
if coord is None or coord.getMicName() is None:
return '%05d' % particle.getMicId()
else:
return pwutils.removeExt(coord.getMicName())
def _getLocation(particle):
if imagesStack is not None:
mic = singleMic
else:
micKey = _getMicKey(particle)
if micKey not in micDict:
mic = Micrograph()
mic.setFileName(join(imagesPrefix, 'particles_%s.mrc' % micKey))
mic.counter = pwobj.Integer(0)
micDict[micKey] = mic
else:
mic = micDict[micKey]
# Count one more particle assigned to this micrograph
mic.counter.increment()
return (mic.counter.get(), mic.getFileName())
ih = ImageHandler()
partAlign = partSet.getAlignment()
for particle in partSet:
if writeImages:
newLoc = _getLocation(particle)
ih.convert(particle, newLoc)
localFn = basename(newLoc[1])
particle.setLocation(newLoc[0], localFn)
emxObj = _particleToEmx(emxData, particle, micSet, partAlign)
else:
emxObj = _coordinateToEmx(emxData, particle, micSet)
emxData.addObject(emxObj)
def _getLocation(particle):
if imagesStack is not None:
mic = singleMic
else:
micKey = _getMicKey(particle)
if micKey not in micDict:
mic = Micrograph()
mic.setFileName(join(imagesPrefix, 'particles_%s.mrc' % micKey))
mic.counter = pwobj.Integer(0)
micDict[micKey] = mic
else:
mic = micDict[micKey]
# Count one more particle assigned to this micrograph
mic.counter.increment()
return (mic.counter.get(), mic.getFileName())
def _getLocation(particle):
if imagesStack is not None:
mic = singleMic
else:
micKey = _getMicKey(particle)
if micKey not in micDict:
mic = Micrograph()
mic.setFileName(join(imagesPrefix,
'particles_%s.mrc' % micKey))
mic.counter = pwobj.Integer(0)
micDict[micKey] = mic
else:
mic = micDict[micKey]
# Count one more particle assigned to this micrograph
mic.counter.increment()
return (mic.counter.get(), mic.getFileName())
