def find_steadystate(ode, BCL, dt, num_of_beats=50, path="../ode/"):
"""
Find a quasi steady state by pacing the 0D cell model at a given cycle length
for a given number of beats.
"""
# Compile the ODE solver module
module, forward = create_module(ode, path=path)
# Fetch model parameter list and init states
model_params = module.init_parameter_values()
states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
# Set the BCL
model_params[BCL_index] = BCL
# Start array for recording results
V = [states[V_index]]
# Solve ODE system over time
t = 0.
tstop = num_of_beats * BCL
while t <= tstop:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
# Save the final state as the steady state
sspath = "../data/steadystates/"
np.save(sspath + "steadystate_%s_BCL%d" % (ode, BCL), states)
return states
def find_steadystates(ode, BCL_range, dt, num_of_beats=50, path="../ode/"):
"""
Find the quasi steady state for a range of cycle lengths by pacing
the 0D cell model at a given cycle length for a given number of beats,
the default being 50 beats.
"""
# Compile the ODE solver module
module, forward = create_module(ode, path=path)
# Fetch model parameter list and init states
model_params = module.init_parameter_values()
init_states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
for BCL in BCL_range:
print "Pacing for BCL %d." % BCL
# Load initial condition
states = init_states
# Set the BCL
model_params[BCL_index] = BCL
# Solve ODE system for given number of beats
t = 0.; tstop = num_of_beats*BCL
while t <= tstop:
forward(states, t, dt, model_params)
t += dt
# Save the final state as the steady state
np.save("../data/steadystates/steadystate_%s_BCL%d" % (ode, BCL), states)
def find_steadystate(ode, BCL, dt, num_of_beats=50, path="../ode/"):
"""
Find a quasi steady state by pacing the 0D cell model at a given cycle length
for a given number of beats.
"""
# Compile the ODE solver module
module, forward = create_module(ode, path=path)
# Fetch model parameter list and init states
model_params = module.init_parameter_values()
states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
# Set the BCL
model_params[BCL_index] = BCL
# Start array for recording results
V = [states[V_index]]
# Solve ODE system over time
t = 0.
tstop = num_of_beats*BCL
while t <= tstop:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
# Save the final state as the steady state
sspath = "../data/steadystates/"
np.save(sspath+"steadystate_%s_BCL%d" % (ode, BCL), states)
return states
def find_steadystates(ode, BCL_range, dt, num_of_beats=50, path="../ode/"):
"""
Find the quasi steady state for a range of cycle lengths by pacing
the 0D cell model at a given cycle length for a given number of beats,
the default being 50 beats.
"""
# Compile the ODE solver module
module, forward = create_module(ode, path=path)
# Fetch model parameter list and init states
model_params = module.init_parameter_values()
init_states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
for BCL in BCL_range:
print "Pacing for BCL %d." % BCL
# Load initial condition
states = init_states
# Set the BCL
model_params[BCL_index] = BCL
# Solve ODE system for given number of beats
t = 0.
tstop = num_of_beats * BCL
while t <= tstop:
forward(states, t, dt, model_params)
t += dt
# Save the final state as the steady state
np.save("../data/steadystates/steadystate_%s_BCL%d" % (ode, BCL),
states)
def erp(ode, BCL_range, dt, start, threshold=0.8):
"""
Calculate the effective refractatory period of an ode model for
a range of BCLs. First the cell is paced for 50 cycles at the
given S1 BCL, then a S2 pulse of a lower cycle length is issued.
If the action potential from the S2 pulse has a peak value of
less than the threshold ratio of the S1 peak, the cell is refractatory.
The ERP is the smallest S2 cycle length where the cell is not refractatory.
"""
module, forward = create_module(ode)
model_params = module.init_parameter_values()
init_states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
results = np.zeros((2, len(BCL_range)))
for i, BCL in enumerate(BCL_range):
# First pace cell for 50 cycles at S1
try:
sspath = "../data/steadystates/"
S1 = np.load(sspath + "steadystate_%s_BCL%d.npy" % (ode, BCL))
except:
print "Pacing for BCL %d." % BCL
# Load initial condition
states = init_states
# Set the BCL
model_params[BCL_index] = BCL
# Solve ODE system for given number of beats
t = 0.
tstop = 50 * BCL
while t <= tstop:
forward(states, t, dt, model_params)
t += dt
# Save the final state as the steady state
np.save("../data/steadystates/steadystate_%s_BCL%d" % (ode, BCL),
states)
S1 = states.copy()
if i == 0:
S2_range = np.arange(start, BCL, 0.1)
else:
S2_range = np.arange(results[1][i - 1], BCL, 0.1)
for S2 in S2_range:
print "Trying %g" % S2
states = S1.copy()
model_params[BCL_index] = S2
V = []
t = 0
tstop = 2 * S2
while t <= S2:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
Vpeak = max(V)
V = []
while t <= tstop:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
if max(V) > 0.8 * Vpeak:
break
print "BCL: %g ERP: %s" % (BCL, S2)
results[0][i] = BCL
results[1][i] = S2
np.save("../data/results/erp_%s" % ode, results)
plt.xlabel(r"Time [ms]", fontsize=20)
plt.ylabel(r"V [mV]", fontsize=20)
#plt.title(r'MAP', fontsize=20)
plt.grid()
plt.legend([u"Koivumäki nSR", u"FK nSR"], prop={'size':20})
plt.savefig('../fig/compare_MAPS_nSR.png')
plt.show()
plt.close()
'''
# cAF
odes = ['hAM_KSMT_cAF', 'FK_cAF']
for i, ode in enumerate(odes):
module, forward = create_module(ode)
model_params = module.init_parameter_values()
states = module.init_state_values()
V_index = module.state_indices("V")
offset_index = module.parameter_indices("stim_offset")
model_params[offset_index] = 10
# Start array for recording results
V = [states[V_index]]
t = 0; tstop = 400
while t <= tstop:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
def pacing_dynamic(ode, BCL_range, dt, threshold=-60):
"""
Calculate a restituion curve using dynamic pacing. The ODE model
is paced for 50 beats at every BCL value, and APD and DI are measured
for the final beat.
"""
# Create ODE solver
module, forward = create_module(ode)
# Get model parameters and initial conditions
model_params = module.init_parameter_values()
init_states = module.init_state_values()
# Get parameter indices
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
# For storing the results
results = np.zeros((3, len(BCL_range)))
for i, BCL in enumerate(BCL_range):
# Set BCL
model_params[BCL_index] = BCL
# Read in steady state from file
try:
sspath = "../data/steadystates/"
states = np.load(sspath+"steadystate_%s_BCL%d.npy" % (ode, BCL))
except:
print "Pacing for BCL %d." % BCL
# Load initial condition
states = init_states
# Solve ODE system for given number of beats
num_of_beats = 50
t = 0.; tstop = num_of_beats*BCL
while t <= tstop:
forward(states, t, dt, model_params)
t += dt
# Save the final state as the steady state
np.save(sspath+"steadystate_%s_BCL%d" % (ode, BCL), states)
# Used to measure when potential crosses threshold
cross_threshold = []
Vp = states[V_index]
t=0.; tstop = BCL;
while t < tstop:
forward(states, t, dt, model_params)
V = states[V_index]
if (V-threshold)*(Vp-threshold) < 0:
cross_threshold.append(t)
Vp = V
t += dt
try:
APD = cross_threshold[-1] - cross_threshold[-2]
DI = tstop - cross_threshold[-1]
except:
APD = 0
DI = 0
print "BCL: %g, APD: %g, DI: %g" % (BCL, APD, DI)
results[0][i] = BCL
results[1][i] = APD
results[2][i] = DI
np.save("../data/results/dynamic_%s" % ode, results)
def pacing_S1S2(ode, S1, S2_range, dt, threshold=-60):
"""
Calculate a restitution curve using S1-S2 pacing, take in
the relevant ode model, the S1 length and the range of S2
values to calculate APD/DI. Note that the steady state of
a BCL=S1 must be lying in /data/. If this does not exist
for the given ODE file, you can make it using steadystate.py.
"""
module, forward = create_module(ode)
model_params = module.init_parameter_values()
# Pace 0D cell model and find quasi-steady state
try:
sspath = "../data/steadystates/"
init_states = np.load(sspath+"steadystate_%s_BCL%d.npy" % (ode, S1))
except:
print "Pacing 0D model for BCL %g..." % S1,
init_states = find_steadystate(S1, 50, dt, ode, plot_results=False)
print "done."
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
def pulse_S2(S2):
"""Pulse the steady state with a single S2 pulse and measure APD/DI."""
states = init_states
model_params[BCL_index] = S2
t = 0.; tstop = 2*S2
while t <= S2:
forward(states, t, dt, model_params)
t += dt
Vp = states[V_index]
cross_threshold = []
while t <= tstop:
forward(states, t, dt, model_params)
V = states[V_index]
if (Vp-threshold)*(V-threshold) < 0:
cross_threshold.append(t)
Vp = V
t += dt
try:
APD = cross_threshold[-1] - cross_threshold[-2]
DI = tstop - cross_threshold[-1]
except:
APD = 0
DI = 0
return APD, DI
results = np.zeros((3, len(S2_range)))
for S2 in S2_range:
APD, DI = pulse_S2(S2)
results[0][i] = S2
results[1][i] = APD
results[2][i] = DI
print "S2: %g, APD: %g, DI: %g" % (S2, APD, DI)
np.save("../data/results/S1S2_%s" % ode, results)
def pacing_dynamic(ode, BCL_range, dt, threshold=-60):
"""
Calculate a restituion curve using dynamic pacing. The ODE model
is paced for 50 beats at every BCL value, and APD and DI are measured
for the final beat.
"""
# Create ODE solver
module, forward = create_module(ode)
# Get model parameters and initial conditions
model_params = module.init_parameter_values()
init_states = module.init_state_values()
# Get parameter indices
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
# For storing the results
results = np.zeros((3, len(BCL_range)))
for i, BCL in enumerate(BCL_range):
# Set BCL
model_params[BCL_index] = BCL
# Read in steady state from file
try:
sspath = "../data/steadystates/"
states = np.load(sspath + "steadystate_%s_BCL%d.npy" % (ode, BCL))
except:
print "Pacing for BCL %d." % BCL
# Load initial condition
states = init_states
# Solve ODE system for given number of beats
num_of_beats = 50
t = 0.
tstop = num_of_beats * BCL
while t <= tstop:
forward(states, t, dt, model_params)
t += dt
# Save the final state as the steady state
np.save(sspath + "steadystate_%s_BCL%d" % (ode, BCL), states)
# Used to measure when potential crosses threshold
cross_threshold = []
Vp = states[V_index]
t = 0.
tstop = BCL
while t < tstop:
forward(states, t, dt, model_params)
V = states[V_index]
if (V - threshold) * (Vp - threshold) < 0:
cross_threshold.append(t)
Vp = V
t += dt
try:
APD = cross_threshold[-1] - cross_threshold[-2]
DI = tstop - cross_threshold[-1]
except:
APD = 0
DI = 0
print "BCL: %g, APD: %g, DI: %g" % (BCL, APD, DI)
results[0][i] = BCL
results[1][i] = APD
results[2][i] = DI
np.save("../data/results/dynamic_%s" % ode, results)
plt.xlabel(r"Time [ms]", fontsize=20)
plt.ylabel(r"V [mV]", fontsize=20)
#plt.title(r'MAP', fontsize=20)
plt.grid()
plt.legend([u"Koivumäki nSR", u"FK nSR"], prop={'size':20})
plt.savefig('../fig/compare_MAPS_nSR.png')
plt.show()
plt.close()
'''
# cAF
odes = ['hAM_KSMT_cAF', 'FK_cAF']
for i, ode in enumerate(odes):
module, forward = create_module(ode)
model_params = module.init_parameter_values()
states = module.init_state_values()
V_index = module.state_indices("V")
offset_index = module.parameter_indices("stim_offset")
model_params[offset_index] = 10
# Start array for recording results
V = [states[V_index]]
t = 0
tstop = 400
while t <= tstop:
forward(states, t, dt, model_params)
V.append(states[V_index])
def erp(ode, BCL_range, dt, start, threshold=0.8):
"""
Calculate the effective refractatory period of an ode model for
a range of BCLs. First the cell is paced for 50 cycles at the
given S1 BCL, then a S2 pulse of a lower cycle length is issued.
If the action potential from the S2 pulse has a peak value of
less than the threshold ratio of the S1 peak, the cell is refractatory.
The ERP is the smallest S2 cycle length where the cell is not refractatory.
"""
module, forward = create_module(ode)
model_params = module.init_parameter_values()
init_states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
results = np.zeros((2,len(BCL_range)))
for i, BCL in enumerate(BCL_range):
# First pace cell for 50 cycles at S1
try:
sspath = "../data/steadystates/"
S1 = np.load(sspath+"steadystate_%s_BCL%d.npy" % (ode, BCL))
except:
print "Pacing for BCL %d." % BCL
# Load initial condition
states = init_states
# Set the BCL
model_params[BCL_index] = BCL
# Solve ODE system for given number of beats
t = 0.; tstop = 50*BCL
while t <= tstop:
forward(states, t, dt, model_params)
t += dt
# Save the final state as the steady state
np.save("../data/steadystates/steadystate_%s_BCL%d" % (ode, BCL), states)
S1 = states.copy()
if i == 0:
S2_range = np.arange(start, BCL, 0.1)
else:
S2_range = np.arange(results[1][i-1], BCL, 0.1)
for S2 in S2_range:
print "Trying %g" % S2
states = S1.copy()
model_params[BCL_index] = S2
V = []
t = 0; tstop = 2*S2
while t <= S2:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
Vpeak = max(V)
V = []
while t <= tstop:
forward(states, t, dt, model_params)
V.append(states[V_index])
t += dt
if max(V) > 0.8*Vpeak:
break
print "BCL: %g ERP: %s" % (BCL, S2)
results[0][i] = BCL
results[1][i] = S2
np.save("../data/results/erp_%s" % ode, results)
def pacing_dynamic(ode, BCL_range, dt, pulse_train_length=30e3,
pacing_memory=True, threshold=-60):
"""
Calculate a restitution curve using dynamic pacing, take
in the relevant ode model, the range of BCLs to be used.
A pulse train of 30 seconds is the default. The regime steps
straight from one BCL to the next, meaning it contains pacing memory.
If pacing memory is turned of, we reload the inital states.
"""
module, forward = create_module(ode)
model_params = module.init_parameter_values()
states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
def pulse_train(BCL, init_states):
"""
Run a pulse train on the cell with given BCL and length,
and measure the APD and DI from the last pulse.
"""
states = init_states
model_params[BCL_index] = BCL
t=0.;
if pulse_train_length > BCL*50.:
tstop = np.ceil(pulse_train_length/BCL)*BCL
else:
tstop = BCL*50.
while t < tstop - BCL:
forward(states, t, dt, model_params)
t += dt
cross_threshold = []
Vp = states[V_index]
while t <= tstop:
forward(states, t, dt, model_params)
V = states[V_index]
if (V-threshold)*(Vp-threshold) < 0:
cross_threshold.append(t)
Vp = V
t += dt
APD = cross_threshold[-1] - cross_threshold[-2]
DI = tstop - cross_threshold[-1]
return APD, DI, states
results_BCL = []
results_APD = []
results_DI = []
prev_states = init_states
for BCL in BCL_range:
states = prev_states if pacing_memory else init_states
APD, DI, prev_states = pulse_train(BCL, states)
results_BCL.append(BCL)
results_APD.append(APD)
results_DI.append(DI)
print "BCL: %g, APD: %g, DI: %g" % (BCL, APD, DI)
np.save("../data/resultss_dynamic_%s" % ode,
np.array((results_BCL, results_APD, results_DI)))
def pacing_dynamic(ode,
BCL_range,
dt,
pulse_train_length=30e3,
pacing_memory=True,
threshold=-60):
"""
Calculate a restitution curve using dynamic pacing, take
in the relevant ode model, the range of BCLs to be used.
A pulse train of 30 seconds is the default. The regime steps
straight from one BCL to the next, meaning it contains pacing memory.
If pacing memory is turned of, we reload the inital states.
"""
module, forward = create_module(ode)
model_params = module.init_parameter_values()
states = module.init_state_values()
# Find indices of various states/parameters
V_index = module.state_indices("V")
BCL_index = module.parameter_indices("stim_period")
def pulse_train(BCL, init_states):
"""
Run a pulse train on the cell with given BCL and length,
and measure the APD and DI from the last pulse.
"""
states = init_states
model_params[BCL_index] = BCL
t = 0.
if pulse_train_length > BCL * 50.:
tstop = np.ceil(pulse_train_length / BCL) * BCL
else:
tstop = BCL * 50.
while t < tstop - BCL:
forward(states, t, dt, model_params)
t += dt
cross_threshold = []
Vp = states[V_index]
while t <= tstop:
forward(states, t, dt, model_params)
V = states[V_index]
if (V - threshold) * (Vp - threshold) < 0:
cross_threshold.append(t)
Vp = V
t += dt
APD = cross_threshold[-1] - cross_threshold[-2]
DI = tstop - cross_threshold[-1]
return APD, DI, states
results_BCL = []
results_APD = []
results_DI = []
prev_states = init_states
for BCL in BCL_range:
states = prev_states if pacing_memory else init_states
APD, DI, prev_states = pulse_train(BCL, states)
results_BCL.append(BCL)
results_APD.append(APD)
results_DI.append(DI)
print "BCL: %g, APD: %g, DI: %g" % (BCL, APD, DI)
np.save("../data/resultss_dynamic_%s" % ode,
np.array((results_BCL, results_APD, results_DI)))
