class OWDatabasesUpdate(OWWidget):
name = "Databases Update"
description = "Update local systems biology databases."
icon = "../widgets/icons/Databases.svg"
priority = 10
inputs = []
outputs = []
want_main_area = False
def __init__(self, parent=None, signalManager=None,
name="Databases update"):
OWWidget.__init__(self, parent, signalManager, name,
wantMainArea=False)
self.searchString = ""
fbox = gui.widgetBox(self.controlArea, "Filter")
self.completer = TokenListCompleter(
self, caseSensitivity=Qt.CaseInsensitive)
self.lineEditFilter = QLineEdit(textChanged=self.SearchUpdate)
self.lineEditFilter.setCompleter(self.completer)
fbox.layout().addWidget(self.lineEditFilter)
box = gui.widgetBox(self.controlArea, "Files")
self.filesView = QTreeWidget(self)
self.filesView.setHeaderLabels(
["", "Data Source", "Update", "Last Updated", "Size"])
self.filesView.setRootIsDecorated(False)
self.filesView.setUniformRowHeights(True)
self.filesView.setSelectionMode(QAbstractItemView.NoSelection)
self.filesView.setSortingEnabled(True)
self.filesView.sortItems(1, Qt.AscendingOrder)
self.filesView.setItemDelegateForColumn(
0, UpdateOptionsItemDelegate(self.filesView))
self.filesView.model().layoutChanged.connect(self.SearchUpdate)
box.layout().addWidget(self.filesView)
box = gui.widgetBox(self.controlArea, orientation="horizontal")
self.updateButton = gui.button(
box, self, "Update all",
callback=self.UpdateAll,
tooltip="Update all updatable files",
)
self.downloadButton = gui.button(
box, self, "Download all",
callback=self.DownloadFiltered,
tooltip="Download all filtered files shown"
)
self.cancelButton = gui.button(
box, self, "Cancel", callback=self.Cancel,
tooltip="Cancel scheduled downloads/updates."
)
self.retryButton = gui.button(
box, self, "Reconnect", callback=self.RetrieveFilesList
)
self.retryButton.hide()
gui.rubber(box)
self.warning(0)
box = gui.widgetBox(self.controlArea, orientation="horizontal")
gui.rubber(box)
self.infoLabel = QLabel()
self.infoLabel.setAlignment(Qt.AlignCenter)
self.controlArea.layout().addWidget(self.infoLabel)
self.infoLabel.setSizePolicy(QSizePolicy.Expanding, QSizePolicy.Fixed)
self.updateItems = []
self.resize(800, 600)
self.progress = ProgressState(self, maximum=3)
self.progress.valueChanged.connect(self._updateProgress)
self.progress.rangeChanged.connect(self._updateProgress)
self.executor = ThreadExecutor(
threadPool=QThreadPool(maxThreadCount=2)
)
task = Task(self, function=self.RetrieveFilesList)
task.exceptionReady.connect(self.HandleError)
task.start()
self._tasks = []
self._haveProgress = False
def RetrieveFilesList(self):
self.retryButton.hide()
self.warning(0)
self.progress.setRange(0, 3)
task = Task(function=partial(retrieveFilesList, methodinvoke(self.progress, "advance")))
task.resultReady.connect(self.SetFilesList)
task.exceptionReady.connect(self.HandleError)
self.executor.submit(task)
self.setEnabled(False)
def SetFilesList(self, serverInfo):
"""
Set the files to show.
"""
self.setEnabled(True)
localInfo = serverfiles.allinfo()
all_tags = set()
self.filesView.clear()
self.updateItems = []
for item in join_info_dict(localInfo, serverInfo):
tree_item = UpdateTreeWidgetItem(item)
options_widget = UpdateOptionsWidget(item.state)
options_widget.item = item
options_widget.installClicked.connect(
partial(self.SubmitDownloadTask, item.domain, item.filename)
)
options_widget.removeClicked.connect(
partial(self.SubmitRemoveTask, item.domain, item.filename)
)
self.updateItems.append((item, tree_item, options_widget))
all_tags.update(item.tags)
self.filesView.addTopLevelItems(
[tree_item for _, tree_item, _ in self.updateItems]
)
for item, tree_item, options_widget in self.updateItems:
self.filesView.setItemWidget(tree_item, 0, options_widget)
# Add an update button if the file is updateable
if item.state == OUTDATED:
button = QToolButton(
None, text="Update",
maximumWidth=120,
minimumHeight=20,
maximumHeight=20
)
if sys.platform == "darwin":
button.setAttribute(Qt.WA_MacSmallSize)
button.clicked.connect(
partial(self.SubmitDownloadTask, item.domain,
item.filename)
)
self.filesView.setItemWidget(tree_item, 2, button)
self.progress.advance()
self.filesView.setColumnWidth(0, self.filesView.sizeHintForColumn(0))
for column in range(1, 4):
contents_hint = self.filesView.sizeHintForColumn(column)
header_hint = self.filesView.header().sectionSizeHint(column)
width = max(min(contents_hint, 400), header_hint)
self.filesView.setColumnWidth(column, width)
hints = [hint for hint in sorted(all_tags) if not hint.startswith("#")]
self.completer.setTokenList(hints)
self.SearchUpdate()
self.UpdateInfoLabel()
self.toggleButtons()
self.cancelButton.setEnabled(False)
self.progress.setRange(0, 0)
def buttonCheck(self, selected_items, state, button):
for item in selected_items:
if item.state != state:
button.setEnabled(False)
else:
button.setEnabled(True)
break
def toggleButtons(self):
selected_items = [item for item, tree_item, _ in self.updateItems if not tree_item.isHidden()]
self.buttonCheck(selected_items, OUTDATED, self.updateButton)
self.buttonCheck(selected_items, AVAILABLE, self.downloadButton)
def HandleError(self, exception):
if isinstance(exception, ConnectionError):
self.warning(0,
"Could not connect to server! Check your connection "
"and try to reconnect.")
self.SetFilesList({})
self.retryButton.show()
else:
sys.excepthook(type(exception), exception, None)
self.progress.setRange(0, 0)
self.setEnabled(True)
def UpdateInfoLabel(self):
local = [item for item, tree_item, _ in self.updateItems
if item.state != AVAILABLE and not tree_item.isHidden()]
size = sum(float(item.size) for item in local)
onServer = [item for item, tree_item, _ in self.updateItems if not tree_item.isHidden()]
sizeOnServer = sum(float(item.size) for item in onServer)
text = ("%i items, %s (on server: %i items, %s)" %
(len(local),
sizeof_fmt(size),
len(onServer),
sizeof_fmt(sizeOnServer)))
self.infoLabel.setText(text)
def UpdateAll(self):
self.warning(0)
for item, tree_item, _ in self.updateItems:
if item.state == OUTDATED and not tree_item.isHidden():
self.SubmitDownloadTask(item.domain, item.filename)
def DownloadFiltered(self):
# TODO: submit items in the order shown.
for item, tree_item, _ in self.updateItems:
if not tree_item.isHidden() and item.state in \
[AVAILABLE, OUTDATED]:
self.SubmitDownloadTask(item.domain, item.filename)
def SearchUpdate(self, searchString=None):
strings = str(self.lineEditFilter.text()).split()
for item, tree_item, _ in self.updateItems:
hide = not all(UpdateItem_match(item, string)
for string in strings)
tree_item.setHidden(hide)
self.UpdateInfoLabel()
self.toggleButtons()
def SubmitDownloadTask(self, domain, filename):
"""
Submit the (domain, filename) to be downloaded/updated.
"""
self.cancelButton.setEnabled(True)
index = self.updateItemIndex(domain, filename)
_, tree_item, opt_widget = self.updateItems[index]
sf = LocalFiles(serverfiles.PATH, serverfiles.ServerFiles())
task = DownloadTask(domain, filename, sf)
self.progress.adjustRange(0, 100)
pb = ItemProgressBar(self.filesView)
pb.setRange(0, 100)
pb.setTextVisible(False)
task.advanced.connect(pb.advance)
task.advanced.connect(self.progress.advance)
task.finished.connect(pb.hide)
task.finished.connect(self.onDownloadFinished, Qt.QueuedConnection)
task.exception.connect(self.onDownloadError, Qt.QueuedConnection)
self.filesView.setItemWidget(tree_item, 2, pb)
# Clear the text so it does not show behind the progress bar.
tree_item.setData(2, Qt.DisplayRole, "")
pb.show()
# Disable the options widget
opt_widget.setEnabled(False)
self._tasks.append(task)
self.executor.submit(task)
def EndDownloadTask(self, task):
future = task.future()
index = self.updateItemIndex(task.domain, task.filename)
item, tree_item, opt_widget = self.updateItems[index]
self.filesView.removeItemWidget(tree_item, 2)
opt_widget.setEnabled(True)
if future.cancelled():
# Restore the previous state
tree_item.setUpdateItem(item)
opt_widget.setState(item.state)
elif future.exception():
tree_item.setUpdateItem(item)
opt_widget.setState(item.state)
# Show the exception string in the size column.
self.warning(0, "Error while downloading. Check your connection "
"and retry.")
# recreate button for download
button = QToolButton(
None, text="Retry",
maximumWidth=120,
minimumHeight=20,
maximumHeight=20
)
if sys.platform == "darwin":
button.setAttribute(Qt.WA_MacSmallSize)
button.clicked.connect(
partial(self.SubmitDownloadTask, item.domain,
item.filename)
)
self.filesView.setItemWidget(tree_item, 2, button)
else:
# get the new updated info dict and replace the the old item
self.warning(0)
info = serverfiles.info(item.domain, item.filename)
new_item = update_item_from_info(item.domain, item.filename,
info, info)
self.updateItems[index] = (new_item, tree_item, opt_widget)
tree_item.setUpdateItem(new_item)
opt_widget.setState(new_item.state)
self.UpdateInfoLabel()
def SubmitRemoveTask(self, domain, filename):
serverfiles.LOCALFILES.remove(domain, filename)
index = self.updateItemIndex(domain, filename)
item, tree_item, opt_widget = self.updateItems[index]
if item.info_server:
new_item = item._replace(state=AVAILABLE, local=None,
info_local=None)
else:
new_item = item._replace(local=None, info_local=None)
# Disable the options widget. No more actions can be performed
# for the item.
opt_widget.setEnabled(False)
tree_item.setUpdateItem(new_item)
opt_widget.setState(new_item.state)
self.updateItems[index] = (new_item, tree_item, opt_widget)
self.UpdateInfoLabel()
def Cancel(self):
"""
Cancel all pending update/download tasks (that have not yet started).
"""
for task in self._tasks:
task.future().cancel()
def onDeleteWidget(self):
self.Cancel()
self.executor.shutdown(wait=False)
OWWidget.onDeleteWidget(self)
def onDownloadFinished(self):
# on download completed/canceled/error
assert QThread.currentThread() is self.thread()
for task in list(self._tasks):
future = task.future()
if future.done():
self.EndDownloadTask(task)
self._tasks.remove(task)
if not self._tasks:
# Clear/reset the overall progress
self.progress.setRange(0, 0)
self.cancelButton.setEnabled(False)
def onDownloadError(self, exc_info):
sys.excepthook(*exc_info)
self.warning(0, "Error while downloading. Check your connection and "
"retry.")
def updateItemIndex(self, domain, filename):
for i, (item, _, _) in enumerate(self.updateItems):
if item.domain == domain and item.filename == filename:
return i
raise ValueError("%r, %r not in update list" % (domain, filename))
def _updateProgress(self, *args):
rmin, rmax = self.progress.range()
if rmin != rmax:
if not self._haveProgress:
self._haveProgress = True
self.progressBarInit()
self.progressBarSet(self.progress.ratioCompleted() * 100,
processEvents=None)
if rmin == rmax:
self._haveProgress = False
self.progressBarFinished()
class OWGOEnrichmentAnalysis(widget.OWWidget):
name = "GO Browser"
description = "Enrichment analysis for Gene Ontology terms."
icon = "../widgets/icons/GOBrowser.svg"
priority = 2020
inputs = [("Cluster Data", Orange.data.Table,
"setDataset", widget.Single + widget.Default),
("Reference Data", Orange.data.Table,
"setReferenceDataset")]
outputs = [("Data on Selected Genes", Orange.data.Table),
("Data on Unselected Genes", Orange.data.Table),
("Data on Unknown Genes", Orange.data.Table),
("Enrichment Report", Orange.data.Table)]
settingsHandler = settings.DomainContextHandler()
annotationIndex = settings.ContextSetting(0)
geneAttrIndex = settings.ContextSetting(0)
useAttrNames = settings.ContextSetting(False)
geneMatcherSettings = settings.Setting([True, False, False, False])
useReferenceDataset = settings.Setting(False)
aspectIndex = settings.Setting(0)
useEvidenceType = settings.Setting(
{et: True for et in go.evidenceTypesOrdered})
filterByNumOfInstances = settings.Setting(False)
minNumOfInstances = settings.Setting(1)
filterByPValue = settings.Setting(True)
maxPValue = settings.Setting(0.2)
filterByPValue_nofdr = settings.Setting(False)
maxPValue_nofdr = settings.Setting(0.01)
probFunc = settings.Setting(0)
selectionDirectAnnotation = settings.Setting(0)
selectionDisjoint = settings.Setting(0)
selectionAddTermAsClass = settings.Setting(0)
Ready, Initializing, Running = 0, 1, 2
def __init__(self, parent=None):
super().__init__(self, parent)
self.clusterDataset = None
self.referenceDataset = None
self.ontology = None
self.annotations = None
self.loadedAnnotationCode = "---"
self.treeStructRootKey = None
self.probFunctions = [stats.Binomial(), stats.Hypergeometric()]
self.selectedTerms = []
self.selectionChanging = 0
self.__state = OWGOEnrichmentAnalysis.Initializing
self.annotationCodes = []
#############
## GUI
#############
self.tabs = gui.tabWidget(self.controlArea)
## Input tab
self.inputTab = gui.createTabPage(self.tabs, "Input")
box = gui.widgetBox(self.inputTab, "Info")
self.infoLabel = gui.widgetLabel(box, "No data on input\n")
gui.button(box, self, "Ontology/Annotation Info",
callback=self.ShowInfo,
tooltip="Show information on loaded ontology and annotations")
box = gui.widgetBox(self.inputTab, "Organism")
self.annotationComboBox = gui.comboBox(
box, self, "annotationIndex", items=self.annotationCodes,
callback=self._updateEnrichment, tooltip="Select organism")
genebox = gui.widgetBox(self.inputTab, "Gene Names")
self.geneAttrIndexCombo = gui.comboBox(
genebox, self, "geneAttrIndex", callback=self._updateEnrichment,
tooltip="Use this attribute to extract gene names from input data")
self.geneAttrIndexCombo.setDisabled(self.useAttrNames)
cb = gui.checkBox(genebox, self, "useAttrNames", "Use column names",
tooltip="Use column names for gene names",
callback=self._updateEnrichment)
cb.toggled[bool].connect(self.geneAttrIndexCombo.setDisabled)
gui.button(genebox, self, "Gene matcher settings",
callback=self.UpdateGeneMatcher,
tooltip="Open gene matching settings dialog")
self.referenceRadioBox = gui.radioButtonsInBox(
self.inputTab, self, "useReferenceDataset",
["Entire genome", "Reference set (input)"],
tooltips=["Use entire genome for reference",
"Use genes from Referece Examples input signal as reference"],
box="Reference", callback=self._updateEnrichment)
self.referenceRadioBox.buttons[1].setDisabled(True)
gui.radioButtonsInBox(
self.inputTab, self, "aspectIndex",
["Biological process", "Cellular component", "Molecular function"],
box="Aspect", callback=self._updateEnrichment)
## Filter tab
self.filterTab = gui.createTabPage(self.tabs, "Filter")
box = gui.widgetBox(self.filterTab, "Filter GO Term Nodes")
gui.checkBox(box, self, "filterByNumOfInstances", "Genes",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by number of input genes mapped to a term")
ibox = gui.indentedBox(box)
gui.spin(ibox, self, 'minNumOfInstances', 1, 100,
step=1, label='#:', labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Min. number of input genes mapped to a term")
gui.checkBox(box, self, "filterByPValue_nofdr", "p-value",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by term p-value")
gui.doubleSpin(gui.indentedBox(box), self, 'maxPValue_nofdr', 1e-8, 1,
step=1e-8, label='p:', labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Max term p-value")
#use filterByPValue for FDR, as it was the default in prior versions
gui.checkBox(box, self, "filterByPValue", "FDR",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by term FDR")
gui.doubleSpin(gui.indentedBox(box), self, 'maxPValue', 1e-8, 1,
step=1e-8, label='p:', labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Max term p-value")
box = gui.widgetBox(box, "Significance test")
gui.radioButtonsInBox(box, self, "probFunc", ["Binomial", "Hypergeometric"],
tooltips=["Use binomial distribution test",
"Use hypergeometric distribution test"],
callback=self._updateEnrichment)
box = gui.widgetBox(self.filterTab, "Evidence codes in annotation",
addSpace=True)
self.evidenceCheckBoxDict = {}
for etype in go.evidenceTypesOrdered:
ecb = QCheckBox(
etype, toolTip=go.evidenceTypes[etype],
checked=self.useEvidenceType[etype])
ecb.toggled.connect(self.__on_evidenceChanged)
box.layout().addWidget(ecb)
self.evidenceCheckBoxDict[etype] = ecb
## Select tab
self.selectTab = gui.createTabPage(self.tabs, "Select")
box = gui.radioButtonsInBox(
self.selectTab, self, "selectionDirectAnnotation",
["Directly or Indirectly", "Directly"],
box="Annotated genes",
callback=self.ExampleSelection)
box = gui.widgetBox(self.selectTab, "Output", addSpace=True)
gui.radioButtonsInBox(
box, self, "selectionDisjoint",
btnLabels=["All selected genes",
"Term-specific genes",
"Common term genes"],
tooltips=["Outputs genes annotated to all selected GO terms",
"Outputs genes that appear in only one of selected GO terms",
"Outputs genes common to all selected GO terms"],
callback=[self.ExampleSelection,
self.UpdateAddClassButton])
self.addClassCB = gui.checkBox(
box, self, "selectionAddTermAsClass", "Add GO Term as class",
callback=self.ExampleSelection)
# ListView for DAG, and table for significant GOIDs
self.DAGcolumns = ['GO term', 'Cluster', 'Reference', 'p-value',
'FDR', 'Genes', 'Enrichment']
self.splitter = QSplitter(Qt.Vertical, self.mainArea)
self.mainArea.layout().addWidget(self.splitter)
# list view
self.listView = GOTreeWidget(self.splitter)
self.listView.setSelectionMode(QTreeView.ExtendedSelection)
self.listView.setAllColumnsShowFocus(1)
self.listView.setColumnCount(len(self.DAGcolumns))
self.listView.setHeaderLabels(self.DAGcolumns)
self.listView.header().setSectionsClickable(True)
self.listView.header().setSortIndicatorShown(True)
self.listView.setSortingEnabled(True)
self.listView.setItemDelegateForColumn(
6, EnrichmentColumnItemDelegate(self))
self.listView.setRootIsDecorated(True)
self.listView.itemSelectionChanged.connect(self.ViewSelectionChanged)
# table of significant GO terms
self.sigTerms = QTreeWidget(self.splitter)
self.sigTerms.setColumnCount(len(self.DAGcolumns))
self.sigTerms.setHeaderLabels(self.DAGcolumns)
self.sigTerms.setSortingEnabled(True)
self.sigTerms.setSelectionMode(QTreeView.ExtendedSelection)
self.sigTerms.setItemDelegateForColumn(
6, EnrichmentColumnItemDelegate(self))
self.sigTerms.itemSelectionChanged.connect(self.TableSelectionChanged)
self.sigTableTermsSorted = []
self.graph = {}
self.inputTab.layout().addStretch(1)
self.filterTab.layout().addStretch(1)
self.selectTab.layout().addStretch(1)
self.setBlocking(True)
self._executor = ThreadExecutor()
self._init = EnsureDownloaded(
[(taxonomy.Taxonomy.DOMAIN, taxonomy.Taxonomy.FILENAME),
("GO", "taxonomy.pickle")]
)
self._init.finished.connect(self.__initialize_finish)
self._executor.submit(self._init)
def sizeHint(self):
return QSize(1000, 700)
def __initialize_finish(self):
self.setBlocking(False)
try:
self.annotationFiles = listAvailable()
except ConnectTimeout:
self.error(2, "Internet connection error, unable to load data. " + \
"Check connection and create a new GO Browser widget.")
self.filterTab.setEnabled(False)
self.inputTab.setEnabled(False)
self.selectTab.setEnabled(False)
self.listView.setEnabled(False)
self.sigTerms.setEnabled(False)
else:
self.annotationCodes = sorted(self.annotationFiles.keys())
self.annotationComboBox.clear()
self.annotationComboBox.addItems(self.annotationCodes)
self.annotationComboBox.setCurrentIndex(self.annotationIndex)
self.__state = OWGOEnrichmentAnalysis.Ready
def __on_evidenceChanged(self):
for etype, cb in self.evidenceCheckBoxDict.items():
self.useEvidenceType[etype] = cb.isChecked()
self._updateEnrichment()
def UpdateGeneMatcher(self):
"""Open the Gene matcher settings dialog."""
dialog = GeneMatcherDialog(self, defaults=self.geneMatcherSettings, modal=True)
if dialog.exec_() != QDialog.Rejected:
self.geneMatcherSettings = [getattr(dialog, item[0]) for item in dialog.items]
if self.annotations:
self.SetGeneMatcher()
self._updateEnrichment()
def clear(self):
self.infoLabel.setText("No data on input\n")
self.warning(0)
self.warning(1)
self.geneAttrIndexCombo.clear()
self.ClearGraph()
self.send("Data on Selected Genes", None)
self.send("Data on Unselected Genes", None)
self.send("Data on Unknown Genes", None)
self.send("Enrichment Report", None)
def setDataset(self, data=None):
if self.__state == OWGOEnrichmentAnalysis.Initializing:
self.__initialize_finish()
self.closeContext()
self.clear()
self.clusterDataset = data
if data is not None:
domain = data.domain
allvars = domain.variables + domain.metas
self.candidateGeneAttrs = [var for var in allvars if isstring(var)]
self.geneAttrIndexCombo.clear()
for var in self.candidateGeneAttrs:
self.geneAttrIndexCombo.addItem(*gui.attributeItem(var))
taxid = data_hints.get_hint(data, "taxid", "")
code = None
try:
code = go.from_taxid(taxid)
except KeyError:
pass
except Exception as ex:
print(ex)
if code is not None:
filename = "gene_association.%s.tar.gz" % code
if filename in self.annotationFiles.values():
self.annotationIndex = \
[i for i, name in enumerate(self.annotationCodes) \
if self.annotationFiles[name] == filename].pop()
self.useAttrNames = data_hints.get_hint(data, "genesinrows",
self.useAttrNames)
self.openContext(data)
self.geneAttrIndex = min(self.geneAttrIndex,
len(self.candidateGeneAttrs) - 1)
if len(self.candidateGeneAttrs) == 0:
self.useAttrNames = True
self.geneAttrIndex = -1
elif self.geneAttrIndex < len(self.candidateGeneAttrs):
self.geneAttrIndex = len(self.candidateGeneAttrs) - 1
self._updateEnrichment()
def setReferenceDataset(self, data=None):
self.referenceDataset = data
self.referenceRadioBox.buttons[1].setDisabled(not bool(data))
self.referenceRadioBox.buttons[1].setText("Reference set")
if self.clusterDataset is not None and self.useReferenceDataset:
self.useReferenceDataset = 0 if not data else 1
graph = self.Enrichment()
self.SetGraph(graph)
elif self.clusterDataset:
self.__updateReferenceSetButton()
def handleNewSignals(self):
super().handleNewSignals()
def _updateEnrichment(self):
if self.clusterDataset is not None and \
self.__state == OWGOEnrichmentAnalysis.Ready:
pb = gui.ProgressBar(self, 100)
self.Load(pb=pb)
graph = self.Enrichment(pb=pb)
self.FilterUnknownGenes()
self.SetGraph(graph)
def __updateReferenceSetButton(self):
allgenes, refgenes = None, None
if self.referenceDataset:
try:
allgenes = self.genesFromTable(self.referenceDataset)
except Exception:
allgenes = []
refgenes, unknown = self.FilterAnnotatedGenes(allgenes)
self.referenceRadioBox.buttons[1].setDisabled(not bool(allgenes))
self.referenceRadioBox.buttons[1].setText("Reference set " + ("(%i genes, %i matched)" % (len(allgenes), len(refgenes)) if allgenes and refgenes else ""))
def genesFromTable(self, data):
if self.useAttrNames:
genes = [v.name for v in data.domain.variables]
else:
attr = self.candidateGeneAttrs[min(self.geneAttrIndex, len(self.candidateGeneAttrs) - 1)]
genes = [str(ex[attr]) for ex in data if not numpy.isnan(ex[attr])]
if any("," in gene for gene in genes):
self.information(0, "Separators detected in gene names. Assuming multiple genes per example.")
genes = reduce(operator.iadd, (genes.split(",") for genes in genes), [])
return genes
def FilterAnnotatedGenes(self, genes):
matchedgenes = self.annotations.get_gene_names_translator(genes).values()
return matchedgenes, [gene for gene in genes if gene not in matchedgenes]
def FilterUnknownGenes(self):
if not self.useAttrNames and self.candidateGeneAttrs:
geneAttr = self.candidateGeneAttrs[min(self.geneAttrIndex, len(self.candidateGeneAttrs)-1)]
indices = []
for i, ex in enumerate(self.clusterDataset):
if not any(self.annotations.genematcher.match(n.strip()) for n in str(ex[geneAttr]).split(",")):
indices.append(i)
if indices:
data = self.clusterDataset[indices]
else:
data = None
self.send("Data on Unknown Genes", data)
else:
self.send("Data on Unknown Genes", None)
def Load(self, pb=None):
if self.__state == OWGOEnrichmentAnalysis.Ready:
go_files, tax_files = serverfiles.listfiles("GO"), serverfiles.listfiles("Taxonomy")
calls = []
pb, finish = (gui.ProgressBar(self, 0), True) if pb is None else (pb, False)
count = 0
if not tax_files:
calls.append(("Taxonomy", "ncbi_taxnomy.tar.gz"))
count += 1
org = self.annotationCodes[min(self.annotationIndex, len(self.annotationCodes)-1)]
if org != self.loadedAnnotationCode:
count += 1
if self.annotationFiles[org] not in go_files:
calls.append(("GO", self.annotationFiles[org]))
count += 1
if "gene_ontology_edit.obo.tar.gz" not in go_files:
calls.append(("GO", "gene_ontology_edit.obo.tar.gz"))
count += 1
if not self.ontology:
count += 1
pb.iter += count * 100
for args in calls:
serverfiles.localpath_download(*args, **dict(callback=pb.advance))
i = len(calls)
if not self.ontology:
self.ontology = go.Ontology(progress_callback=lambda value: pb.advance())
i += 1
if org != self.loadedAnnotationCode:
self.annotations = None
gc.collect() # Force run garbage collection
code = self.annotationFiles[org].split(".")[-3]
self.annotations = go.Annotations(code, genematcher=gene.GMDirect(), progress_callback=lambda value: pb.advance())
i += 1
self.loadedAnnotationCode = org
count = defaultdict(int)
geneSets = defaultdict(set)
for anno in self.annotations.annotations:
count[anno.evidence] += 1
geneSets[anno.evidence].add(anno.geneName)
for etype in go.evidenceTypesOrdered:
ecb = self.evidenceCheckBoxDict[etype]
ecb.setEnabled(bool(count[etype]))
ecb.setText(etype + ": %i annots(%i genes)" % (count[etype], len(geneSets[etype])))
if finish:
pb.finish()
def SetGeneMatcher(self):
if self.annotations:
taxid = self.annotations.taxid
matchers = []
for matcher, use in zip([gene.GMGO, gene.GMKEGG, gene.GMNCBI, gene.GMAffy], self.geneMatcherSettings):
if use:
try:
if taxid == "352472":
matchers.extend([matcher(taxid), gene.GMDicty(),
[matcher(taxid), gene.GMDicty()]])
# The reason machers are duplicated is that we want `matcher` or `GMDicty` to
# match genes by them self if possible. Only use the joint matcher if they fail.
else:
matchers.append(matcher(taxid))
except Exception as ex:
print(ex)
self.annotations.genematcher = gene.matcher(matchers)
self.annotations.genematcher.set_targets(self.annotations.gene_names)
def Enrichment(self, pb=None):
assert self.clusterDataset is not None
pb = gui.ProgressBar(self, 100) if pb is None else pb
if not self.annotations.ontology:
self.annotations.ontology = self.ontology
if isinstance(self.annotations.genematcher, gene.GMDirect):
self.SetGeneMatcher()
self.error(1)
self.warning([0, 1])
if self.useAttrNames:
clusterGenes = [v.name for v in self.clusterDataset.domain.attributes]
self.information(0)
elif 0 <= self.geneAttrIndex < len(self.candidateGeneAttrs):
geneAttr = self.candidateGeneAttrs[self.geneAttrIndex]
clusterGenes = [str(ex[geneAttr]) for ex in self.clusterDataset
if not numpy.isnan(ex[geneAttr])]
if any("," in gene for gene in clusterGenes):
self.information(0, "Separators detected in cluster gene names. Assuming multiple genes per example.")
clusterGenes = reduce(operator.iadd, (genes.split(",") for genes in clusterGenes), [])
else:
self.information(0)
else:
self.error(1, "Failed to extract gene names from input dataset!")
return {}
genesSetCount = len(set(clusterGenes))
self.clusterGenes = clusterGenes = self.annotations.get_gene_names_translator(clusterGenes).values()
self.infoLabel.setText("%i unique genes on input\n%i (%.1f%%) genes with known annotations" % (genesSetCount, len(clusterGenes), 100.0*len(clusterGenes)/genesSetCount if genesSetCount else 0.0))
referenceGenes = None
if not self.useReferenceDataset or self.referenceDataset is None:
self.information(2)
self.information(1)
referenceGenes = self.annotations.gene_names
elif self.referenceDataset is not None:
if self.useAttrNames:
referenceGenes = [v.name for v in self.referenceDataset.domain.attributes]
self.information(1)
elif geneAttr in (self.referenceDataset.domain.variables +
self.referenceDataset.domain.metas):
referenceGenes = [str(ex[geneAttr]) for ex in self.referenceDataset
if not numpy.isnan(ex[geneAttr])]
if any("," in gene for gene in clusterGenes):
self.information(1, "Separators detected in reference gene names. Assuming multiple genes per example.")
referenceGenes = reduce(operator.iadd, (genes.split(",") for genes in referenceGenes), [])
else:
self.information(1)
else:
self.information(1)
referenceGenes = None
if referenceGenes is None:
referenceGenes = list(self.annotations.gene_names)
self.referenceRadioBox.buttons[1].setText("Reference set")
self.referenceRadioBox.buttons[1].setDisabled(True)
self.information(2, "Unable to extract gene names from reference dataset. Using entire genome for reference")
self.useReferenceDataset = 0
else:
refc = len(referenceGenes)
referenceGenes = self.annotations.get_gene_names_translator(referenceGenes).values()
self.referenceRadioBox.buttons[1].setText("Reference set (%i genes, %i matched)" % (refc, len(referenceGenes)))
self.referenceRadioBox.buttons[1].setDisabled(False)
self.information(2)
else:
self.useReferenceDataset = 0
if not referenceGenes:
self.error(1, "No valid reference set")
return {}
self.referenceGenes = referenceGenes
evidences = []
for etype in go.evidenceTypesOrdered:
if self.useEvidenceType[etype]:
evidences.append(etype)
aspect = ["P", "C", "F"][self.aspectIndex]
if clusterGenes:
self.terms = terms = self.annotations.get_enriched_terms(
clusterGenes, referenceGenes, evidences, aspect=aspect,
prob=self.probFunctions[self.probFunc], use_fdr=False,
progress_callback=lambda value: pb.advance())
ids = []
pvals = []
for i, d in self.terms.items():
ids.append(i)
pvals.append(d[1])
for i, fdr in zip(ids, stats.FDR(pvals)): # save FDR as the last part of the tuple
terms[i] = tuple(list(terms[i]) + [ fdr ])
else:
self.terms = terms = {}
if not self.terms:
self.warning(0, "No enriched terms found.")
else:
self.warning(0)
pb.finish()
self.treeStructDict = {}
ids = self.terms.keys()
self.treeStructRootKey = None
parents = {}
for id in ids:
parents[id] = set([term for _, term in self.ontology[id].related])
children = {}
for term in self.terms:
children[term] = set([id for id in ids if term in parents[id]])
for term in self.terms:
self.treeStructDict[term] = TreeNode(self.terms[term], children[term])
if not self.ontology[term].related and not getattr(self.ontology[term], "is_obsolete", False):
self.treeStructRootKey = term
return terms
def FilterGraph(self, graph):
if self.filterByPValue_nofdr:
graph = go.filterByPValue(graph, self.maxPValue_nofdr)
if self.filterByPValue: #FDR
graph = dict(filter(lambda item: item[1][3] <= self.maxPValue, graph.items()))
if self.filterByNumOfInstances:
graph = dict(filter(lambda item: len(item[1][0]) >= self.minNumOfInstances, graph.items()))
return graph
def FilterAndDisplayGraph(self):
if self.clusterDataset:
self.graph = self.FilterGraph(self.originalGraph)
if self.originalGraph and not self.graph:
self.warning(1, "All found terms were filtered out.")
else:
self.warning(1)
self.ClearGraph()
self.DisplayGraph()
def SetGraph(self, graph=None):
self.originalGraph = graph
if graph:
self.FilterAndDisplayGraph()
else:
self.graph = {}
self.ClearGraph()
def ClearGraph(self):
self.listView.clear()
self.listViewItems=[]
self.sigTerms.clear()
def DisplayGraph(self):
fromParentDict = {}
self.termListViewItemDict = {}
self.listViewItems = []
enrichment = lambda t: len(t[0]) / t[2] * (len(self.referenceGenes) / len(self.clusterGenes))
maxFoldEnrichment = max([enrichment(term) for term in self.graph.values()] or [1])
def addNode(term, parent, parentDisplayNode):
if (parent, term) in fromParentDict:
return
if term in self.graph:
displayNode = GOTreeWidgetItem(self.ontology[term], self.graph[term], len(self.clusterGenes), len(self.referenceGenes), maxFoldEnrichment, parentDisplayNode)
displayNode.goId = term
self.listViewItems.append(displayNode)
if term in self.termListViewItemDict:
self.termListViewItemDict[term].append(displayNode)
else:
self.termListViewItemDict[term] = [displayNode]
fromParentDict[(parent, term)] = True
parent = term
else:
displayNode = parentDisplayNode
for c in self.treeStructDict[term].children:
addNode(c, parent, displayNode)
if self.treeStructDict:
addNode(self.treeStructRootKey, None, self.listView)
terms = self.graph.items()
terms = sorted(terms, key=lambda item: item[1][1])
self.sigTableTermsSorted = [t[0] for t in terms]
self.sigTerms.clear()
for i, (t_id, (genes, p_value, refCount, fdr)) in enumerate(terms):
item = GOTreeWidgetItem(self.ontology[t_id],
(genes, p_value, refCount, fdr),
len(self.clusterGenes),
len(self.referenceGenes),
maxFoldEnrichment,
self.sigTerms)
item.goId = t_id
self.listView.expandAll()
for i in range(5):
self.listView.resizeColumnToContents(i)
self.sigTerms.resizeColumnToContents(i)
self.sigTerms.resizeColumnToContents(6)
width = min(self.listView.columnWidth(0), 350)
self.listView.setColumnWidth(0, width)
self.sigTerms.setColumnWidth(0, width)
# Create and send the enrichemnt report table.
termsDomain = Orange.data.Domain(
[], [],
# All is meta!
[Orange.data.StringVariable("GO Term Id"),
Orange.data.StringVariable("GO Term Name"),
Orange.data.ContinuousVariable("Cluster Frequency"),
Orange.data.ContinuousVariable("Genes in Cluster", number_of_decimals=0),
Orange.data.ContinuousVariable("Reference Frequency"),
Orange.data.ContinuousVariable("Genes in Reference", number_of_decimals=0),
Orange.data.ContinuousVariable("p-value"),
Orange.data.ContinuousVariable("FDR"),
Orange.data.ContinuousVariable("Enrichment"),
Orange.data.StringVariable("Genes")])
terms = [[t_id,
self.ontology[t_id].name,
len(genes) / len(self.clusterGenes),
len(genes),
r_count / len(self.referenceGenes),
r_count,
p_value,
fdr,
len(genes) / len(self.clusterGenes) * \
len(self.referenceGenes) / r_count,
",".join(genes)
]
for t_id, (genes, p_value, r_count, fdr) in terms]
if terms:
X = numpy.empty((len(terms), 0))
M = numpy.array(terms, dtype=object)
termsTable = Orange.data.Table.from_numpy(termsDomain, X, metas=M)
else:
termsTable = Orange.data.Table(termsDomain)
self.send("Enrichment Report", termsTable)
def ViewSelectionChanged(self):
if self.selectionChanging:
return
self.selectionChanging = 1
self.selectedTerms = []
selected = self.listView.selectedItems()
self.selectedTerms = list(set([lvi.term.id for lvi in selected]))
self.ExampleSelection()
self.selectionChanging = 0
def TableSelectionChanged(self):
if self.selectionChanging:
return
self.selectionChanging = 1
self.selectedTerms = []
selectedIds = set([self.sigTerms.itemFromIndex(index).goId for index in self.sigTerms.selectedIndexes()])
for i in range(self.sigTerms.topLevelItemCount()):
item = self.sigTerms.topLevelItem(i)
selected = item.goId in selectedIds
term = item.goId
if selected:
self.selectedTerms.append(term)
for lvi in self.termListViewItemDict[term]:
try:
lvi.setSelected(selected)
if selected:
lvi.setExpanded(True)
except RuntimeError: # Underlying C/C++ object deleted
pass
self.ExampleSelection()
self.selectionChanging = 0
def UpdateAddClassButton(self):
self.addClassCB.setEnabled(self.selectionDisjoint == 1)
def ExampleSelection(self):
self.commit()
def commit(self):
if self.clusterDataset is None:
return
terms = set(self.selectedTerms)
genes = reduce(operator.ior,
(set(self.graph[term][0]) for term in terms), set())
evidences = []
for etype in go.evidenceTypesOrdered:
if self.useEvidenceType[etype]:
# if getattr(self, "useEvidence" + etype):
evidences.append(etype)
allTerms = self.annotations.get_annotated_terms(
genes, direct_annotation_only=self.selectionDirectAnnotation,
evidence_codes=evidences)
if self.selectionDisjoint > 0:
count = defaultdict(int)
for term in self.selectedTerms:
for g in allTerms.get(term, []):
count[g] += 1
ccount = 1 if self.selectionDisjoint == 1 else len(self.selectedTerms)
selectedGenes = [gene for gene, c in count.items()
if c == ccount and gene in genes]
else:
selectedGenes = reduce(
operator.ior,
(set(allTerms.get(term, [])) for term in self.selectedTerms),
set())
if self.useAttrNames:
vars = [self.clusterDataset.domain[gene]
for gene in set(selectedGenes)]
domain = Orange.data.Domain(
vars, self.clusterDataset.domain.class_vars,
self.clusterDataset.domain.metas)
newdata = self.clusterDataset.from_table(domain, self.clusterDataset)
self.send("Data on Selected Genes", newdata)
self.send("Data on Unselected Genes", None)
elif self.candidateGeneAttrs:
selectedExamples = []
unselectedExamples = []
geneAttr = self.candidateGeneAttrs[min(self.geneAttrIndex, len(self.candidateGeneAttrs)-1)]
if self.selectionDisjoint == 1:
goVar = Orange.data.DiscreteVariable(
"GO Term", values=list(self.selectedTerms))
newDomain = Orange.data.Domain(
self.clusterDataset.domain.variables, goVar,
self.clusterDataset.domain.metas)
goColumn = []
for i, ex in enumerate(self.clusterDataset):
if not numpy.isnan(ex[geneAttr]) and any(gene in selectedGenes for gene in str(ex[geneAttr]).split(",")):
if self.selectionDisjoint == 1 and self.selectionAddTermAsClass:
terms = filter(lambda term: any(gene in self.graph[term][0] for gene in str(ex[geneAttr]).split(",")) , self.selectedTerms)
term = sorted(terms)[0]
goColumn.append(goVar.values.index(term))
selectedExamples.append(i)
else:
unselectedExamples.append(i)
if selectedExamples:
selectedExamples = self.clusterDataset[selectedExamples]
if self.selectionDisjoint == 1 and self.selectionAddTermAsClass:
selectedExamples = Orange.data.Table.from_table(newDomain, selectedExamples)
view, issparse = selectedExamples.get_column_view(goVar)
assert not issparse
view[:] = goColumn
else:
selectedExamples = None
if unselectedExamples:
unselectedExamples = self.clusterDataset[unselectedExamples]
else:
unselectedExamples = None
self.send("Data on Selected Genes", selectedExamples)
self.send("Data on Unselected Genes", unselectedExamples)
def ShowInfo(self):
dialog = QDialog(self)
dialog.setModal(False)
dialog.setLayout(QVBoxLayout())
label = QLabel(dialog)
label.setText("Ontology:\n" + self.ontology.header
if self.ontology else "Ontology not loaded!")
dialog.layout().addWidget(label)
label = QLabel(dialog)
label.setText("Annotations:\n" + self.annotations.header.replace("!", "")
if self.annotations else "Annotations not loaded!")
dialog.layout().addWidget(label)
dialog.show()
def onDeleteWidget(self):
"""Called before the widget is removed from the canvas.
"""
self.annotations = None
self.ontology = None
gc.collect() # Force collection
class OWDatabasesUpdate(OWWidget):
name = "Databases Update"
description = "Update local systems biology databases."
icon = "../widgets/icons/OWDatabasesUpdate.svg"
priority = 1
inputs = []
outputs = []
want_main_area = False
def __init__(self,
parent=None,
signalManager=None,
name="Databases update"):
OWWidget.__init__(self,
parent,
signalManager,
name,
wantMainArea=False)
self.searchString = ""
fbox = gui.widgetBox(self.controlArea, "Filter")
self.completer = TokenListCompleter(self,
caseSensitivity=Qt.CaseInsensitive)
self.lineEditFilter = QLineEdit(textChanged=self.search_update)
self.lineEditFilter.setCompleter(self.completer)
fbox.layout().addWidget(self.lineEditFilter)
box = gui.widgetBox(self.controlArea, "Files")
self.filesView = QTreeWidget(self)
self.filesView.setHeaderLabels(header_labels)
self.filesView.setRootIsDecorated(False)
self.filesView.setUniformRowHeights(True)
self.filesView.setSelectionMode(QAbstractItemView.NoSelection)
self.filesView.setSortingEnabled(True)
self.filesView.sortItems(header.Title, Qt.AscendingOrder)
self.filesView.setItemDelegateForColumn(
0, UpdateOptionsItemDelegate(self.filesView))
self.filesView.model().layoutChanged.connect(self.search_update)
box.layout().addWidget(self.filesView)
layout = QHBoxLayout()
gui.widgetBox(self.controlArea, margin=0, orientation=layout)
self.updateButton = gui.button(
box,
self,
"Update all",
callback=self.update_all,
tooltip="Update all updatable files",
)
self.downloadButton = gui.button(
box,
self,
"Download all",
callback=self.download_filtered,
tooltip="Download all filtered files shown")
self.cancelButton = gui.button(
box,
self,
"Cancel",
callback=self.cancel_active_threads,
tooltip="Cancel scheduled downloads/updates.")
self.addButton = gui.button(box,
self,
"Add ...",
callback=self.__handle_dialog,
tooltip="Add files for personal use.")
layout.addWidget(self.updateButton)
layout.addWidget(self.downloadButton)
layout.addWidget(self.cancelButton)
layout.addStretch()
layout.addWidget(self.addButton)
# Enable retryButton once connection is established
# self.retryButton = gui.button(
# box, self, "Reconnect", callback=self.initialize_files_view
# )
# self.retryButton.hide()
self.resize(800, 600)
self.update_items = []
self._dialog = None
self.progress_bar = None
# threads
self.threadpool = QThreadPool(self)
#self.threadpool.setMaxThreadCount(1)
self.workers = list()
self.initialize_files_view()
def __handle_dialog(self):
if not self._dialog:
self._dialog = FileUploadHelper(self)
self._dialog.show()
def __progress_advance(self):
# GUI should be updated in main thread. That's why we are calling advance method here
if self.progress_bar:
self.progress_bar.advance()
def handle_worker_exception(self, ex):
self.progress_bar.finish()
self.setStatusMessage('')
if isinstance(ex, ConnectionError):
# TODO: set warning messages
pass
print(ex)
def initialize_files_view(self):
# self.retryButton.hide()
# clear view
self.filesView.clear()
# init progress bar
self.progress_bar = gui.ProgressBar(self, iterations=3)
# status message
self.setStatusMessage('initializing')
worker = Worker(evaluate_files_state, progress_callback=True)
worker.signals.progress.connect(self.__progress_advance)
worker.signals.result.connect(self.set_files_list)
worker.signals.error.connect(self.handle_worker_exception)
# move download process to worker thread
self.threadpool.start(worker)
self.setEnabled(False)
def __create_action_button(self, fs, retry=None):
if not fs.state not in [OUTDATED, USER_FILE] or not retry:
self.filesView.setItemWidget(fs.tree_item, header.Update, None)
button = QToolButton(None)
if not retry:
if fs.state == OUTDATED:
button.setText('Update')
button.clicked.connect(
partial(self.submit_download_task, fs.domain, fs.filename,
True))
elif fs.state == USER_FILE:
if not fs.info_server:
button.setText('Remove')
button.clicked.connect(
partial(self.submit_remove_task, fs.domain,
fs.filename))
else:
button.setText('Use server version')
button.clicked.connect(
partial(self.submit_download_task, fs.domain,
fs.filename, True))
else:
button.setText('Retry')
button.clicked.connect(
partial(self.submit_download_task, fs.domain, fs.filename,
True))
button.setMaximumWidth(120)
button.setMaximumHeight(20)
button.setMinimumHeight(20)
if sys.platform == "darwin":
button.setAttribute(Qt.WA_MacSmallSize)
self.filesView.setItemWidget(fs.tree_item, header.Update, button)
def set_files_list(self, result):
""" Set the files to show.
"""
assert threading.current_thread() == threading.main_thread()
self.progress_bar.finish()
self.setStatusMessage('')
self.setEnabled(True)
self.update_items = result
all_tags = set()
for fs in self.update_items:
fs.tree_item = FileStateItem(fs)
fs.download_option = DownloadOption(state=fs.state)
fs.download_option.download_clicked.connect(
partial(self.submit_download_task, fs.domain, fs.filename))
fs.download_option.remove_clicked.connect(
partial(self.submit_remove_task, fs.domain, fs.filename))
# add widget items to the QTreeWidget
self.filesView.addTopLevelItems(
[fs.tree_item for fs in self.update_items])
# add action widgets to tree items
for fs in self.update_items:
self.filesView.setItemWidget(fs.tree_item, header.Download,
fs.download_option)
if fs.state in [USER_FILE, OUTDATED]:
self.__create_action_button(fs)
all_tags.update(fs.tags)
self.filesView.setColumnWidth(
header.Download, self.filesView.sizeHintForColumn(header.Download))
for column in range(1, len(header_labels)):
self.filesView.resizeColumnToContents(column)
hints = [hint for hint in sorted(all_tags) if not hint.startswith("#")]
self.completer.setTokenList(hints)
self.search_update()
self.toggle_action_buttons()
self.cancelButton.setEnabled(False)
def toggle_action_buttons(self):
selected_items = [
fs for fs in self.update_items if not fs.tree_item.isHidden()
]
def button_check(sel_items, state, button):
for item in sel_items:
if item.state != state:
button.setEnabled(False)
else:
button.setEnabled(True)
break
button_check(selected_items, OUTDATED, self.updateButton)
button_check(selected_items, AVAILABLE, self.downloadButton)
def search_update(self, searchString=None):
strings = str(self.lineEditFilter.text()).split()
for fs in self.update_items:
hide = not all(UpdateItem_match(fs, string) for string in strings)
fs.tree_item.setHidden(hide)
self.toggle_action_buttons()
def update_all(self):
for fs in self.update_items:
if fs.state == OUTDATED and not fs.tree_item.isHidden():
self.submit_download_task(fs.domain, fs.filename)
def download_filtered(self):
for fs in self.update_items:
if not fs.tree_item.isHidden() and fs.state in [
AVAILABLE, OUTDATED
]:
self.submit_download_task(fs.domain, fs.filename, start=False)
self.run_download_tasks()
def submit_download_task(self, domain, filename, start=True):
""" Submit the (domain, filename) to be downloaded/updated.
"""
# get selected tree item
index = self.tree_item_index(domain, filename)
fs = self.update_items[index]
worker = Worker(download_server_file,
fs,
index,
progress_callback=True)
worker.signals.progress.connect(self.__progress_advance)
worker.signals.result.connect(self.on_download_finished)
worker.signals.error.connect(self.on_download_exception)
self.workers.append(worker)
if start:
self.run_download_tasks()
def run_download_tasks(self):
self.cancelButton.setEnabled(True)
# init progress bar
self.progress_bar = gui.ProgressBar(self,
iterations=len(self.workers) * 100)
# status message
self.setStatusMessage('downloading')
# move workers to threadpool
[self.threadpool.start(worker) for worker in self.workers]
self.filesView.setDisabled(True)
# reset list of workers
self.workers = list()
def on_download_exception(self, ex):
assert threading.current_thread() == threading.main_thread()
self.progress_bar.finish()
self.setStatusMessage('')
print(ex)
if isinstance(ex, ValueError):
fs, index = ex.args
# restore state and retry
fs.refresh_state()
fs.tree_item.update_data(fs)
fs.download_option.state = fs.state
self.__create_action_button(fs, retry=True)
def on_download_finished(self, result):
assert threading.current_thread() == threading.main_thread()
# We check if all workers have completed. If not, continue
if self.progress_bar.count == 100 or self.threadpool.activeThreadCount(
) == 0:
self.filesView.setDisabled(False)
self.progress_bar.finish()
self.setStatusMessage('')
fs, index = result
# re-evaluate File State
info = serverfiles.info(fs.domain, fs.filename)
fs.refresh_state(info_local=info, info_server=info)
# reinitialize treeWidgetItem
fs.tree_item.update_data(fs)
# reinitialize OptionWidget
fs.download_option.state = fs.state
self.filesView.setItemWidget(fs.tree_item, header.Update, None)
self.toggle_action_buttons()
for column in range(1, len(header_labels)):
self.filesView.resizeColumnToContents(column)
def submit_remove_task(self, domain, filename):
serverfiles.LOCALFILES.remove(domain, filename)
index = self.tree_item_index(domain, filename)
fs = self.update_items[index]
if fs.state == USER_FILE:
self.filesView.takeTopLevelItem(
self.filesView.indexOfTopLevelItem(fs.tree_item))
self.update_items.remove(fs)
# self.filesView.removeItemWidget(index)
else:
# refresh item state
fs.info_local = None
fs.refresh_state()
# reinitialize treeWidgetItem
fs.tree_item.update_data(fs)
# reinitialize OptionWidget
fs.download_option.state = fs.state
self.toggle_action_buttons()
def cancel_active_threads(self):
""" Cancel all pending update/download tasks (that have not yet started).
"""
if self.threadpool:
self.threadpool.clear()
def tree_item_index(self, domain, filename):
for i, fs in enumerate(self.update_items):
if fs.domain == domain and fs.filename == filename:
return i
raise ValueError("%r, %r not in update list" % (domain, filename))
def onDeleteWidget(self):
self.cancel_active_threads()
OWWidget.onDeleteWidget(self)
class OWPIPAx(widget.OWWidget):
name = "PIPAx"
description = "Access data from PIPA RNA-Seq database."
icon = "../widgets/icons/PIPA.svg"
priority = 35
inputs = []
outputs = [("Data", Orange.data.Table)]
username = settings.Setting("")
password = settings.Setting("")
log2 = settings.Setting(False)
rtypei = settings.Setting(5) # hardcoded rpkm mapability polya
excludeconstant = settings.Setting(False)
joinreplicates = settings.Setting(False)
#: The stored current selection (in experiments view)
#: SelectionByKey | None
currentSelection = settings.Setting(None)
#: Stored selections (presets)
#: list of SelectionByKey
storedSelections = settings.Setting([])
#: Stored column sort keys (from Sort view)
#: list of strings
storedSortingOrder = settings.Setting(
["Strain", "Experiment", "Genotype", "Timepoint"])
experimentsHeaderState = settings.Setting(
{name: False for _, name in HEADER[:ID_INDEX + 1]}
)
def __init__(self, parent=None, signalManager=None, name="PIPAx"):
super().__init__(parent)
self.selectedExperiments = []
self.buffer = dicty.CacheSQLite(bufferfile)
self.searchString = ""
self.result_types = []
self.mappings = {}
self.controlArea.setMaximumWidth(250)
self.controlArea.setMinimumWidth(250)
gui.button(self.controlArea, self, "Reload",
callback=self.Reload)
gui.button(self.controlArea, self, "Clear cache",
callback=self.clear_cache)
b = gui.widgetBox(self.controlArea, "Experiment Sets")
self.selectionSetsWidget = SelectionSetsWidget(self)
self.selectionSetsWidget.setSizePolicy(
QSizePolicy.Preferred, QSizePolicy.Maximum)
def store_selections(modified):
if not modified:
self.storedSelections = self.selectionSetsWidget.selections
self.selectionSetsWidget.selectionModified.connect(store_selections)
b.layout().addWidget(self.selectionSetsWidget)
gui.separator(self.controlArea)
b = gui.widgetBox(self.controlArea, "Sort output columns")
self.columnsSortingWidget = SortedListWidget(self)
self.columnsSortingWidget.setSizePolicy(
QSizePolicy.Preferred, QSizePolicy.Maximum)
def store_sort_order():
self.storedSortingOrder = self.columnsSortingWidget.sortingOrder
self.columnsSortingWidget.sortingOrderChanged.connect(store_sort_order)
b.layout().addWidget(self.columnsSortingWidget)
sorting_model = QStringListModel(SORTING_MODEL_LIST)
self.columnsSortingWidget.setModel(sorting_model)
gui.separator(self.controlArea)
box = gui.widgetBox(self.controlArea, 'Expression Type')
self.expressionTypesCB = gui.comboBox(
box, self, "rtypei", items=[], callback=self.UpdateResultsList)
gui.checkBox(self.controlArea, self, "excludeconstant",
"Exclude labels with constant values")
gui.checkBox(self.controlArea, self, "joinreplicates",
"Average replicates (use median)")
gui.checkBox(self.controlArea, self, "log2",
"Logarithmic (base 2) transformation")
self.commit_button = gui.button(self.controlArea, self, "&Commit",
callback=self.Commit)
self.commit_button.setDisabled(True)
gui.rubber(self.controlArea)
box = gui.widgetBox(self.controlArea, "Authentication")
gui.lineEdit(box, self, "username", "Username:"******"password", "Password:"******"searchString", "Search",
callbackOnType=True,
callback=self.SearchUpdate)
self.headerLabels = [t[1] for t in HEADER]
self.experimentsWidget = QTreeWidget()
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.experimentsWidget.setSelectionMode(QTreeWidget.ExtendedSelection)
self.experimentsWidget.setRootIsDecorated(False)
self.experimentsWidget.setSortingEnabled(True)
contextEventFilter = gui.VisibleHeaderSectionContextEventFilter(
self.experimentsWidget, self.experimentsWidget
)
self.experimentsWidget.header().installEventFilter(contextEventFilter)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.setAlternatingRowColors(True)
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.onSelectionChanged)
self.selectionSetsWidget.setSelectionModel(
self.experimentsWidget.selectionModel()
)
self.mainArea.layout().addWidget(self.experimentsWidget)
# Restore the selection states from the stored settings
self.selectionSetsWidget.selections = self.storedSelections
self.columnsSortingWidget.sortingOrder = self.storedSortingOrder
self.restoreHeaderState()
self.experimentsWidget.header().geometriesChanged.connect(
self.saveHeaderState)
self.dbc = None
self.AuthSet()
QTimer.singleShot(100, self.UpdateExperiments)
def sizeHint(self):
return QSize(800, 600)
def AuthSet(self):
if len(self.username):
self.passf.setDisabled(False)
else:
self.passf.setDisabled(True)
def AuthChanged(self):
self.AuthSet()
self.ConnectAndUpdate()
def ConnectAndUpdate(self):
self.Connect()
self.UpdateExperiments(reload=True)
def Connect(self):
self.error(1)
self.warning(1)
def en(x):
return x if len(x) else None
self.dbc = dicty.PIPAx(cache=self.buffer,
username=en(self.username),
password=self.password)
# check password
if en(self.username) != None:
try:
self.dbc.mappings(reload=True)
except dicty.AuthenticationError:
self.error(1, "Wrong username or password")
self.dbc = None
except Exception as ex:
print("Error when contacting the PIPA database", ex)
sys.excepthook(*sys.exc_info())
try: # maybe cached?
self.dbc.mappings()
self.warning(1, "Can not access database - using cached data.")
except Exception as ex:
self.dbc = None
self.error(1, "Can not access database.")
def Reload(self):
self.UpdateExperiments(reload=True)
def clear_cache(self):
self.buffer.clear()
self.Reload()
def rtype(self):
"""Return selected result template type """
if self.result_types:
return self.result_types[self.rtypei][0]
else:
return "-1"
def UpdateExperimentTypes(self):
self.expressionTypesCB.clear()
items = [desc for _, desc in self.result_types]
self.expressionTypesCB.addItems(items)
self.rtypei = max(0, min(self.rtypei, len(self.result_types) - 1))
def UpdateExperiments(self, reload=False):
self.experimentsWidget.clear()
self.items = []
self.progressBarInit()
if not self.dbc:
self.Connect()
mappings = {}
result_types = []
sucind = False # success indicator for database index
try:
mappings = self.dbc.mappings(reload=reload)
result_types = self.dbc.result_types(reload=reload)
sucind = True
except Exception as ex:
try:
mappings = self.dbc.mappings()
result_types = self.dbc.result_types()
self.warning(0, "Can not access database - using cached data.")
sucind = True
except Exception as ex:
self.error(0, "Can not access database.")
if sucind:
self.warning(0)
self.error(0)
self.mappings = mappings
self.result_types = result_types
self.UpdateExperimentTypes()
self.UpdateResultsList(reload=reload)
self.progressBarFinished()
if self.currentSelection:
self.currentSelection.select(
self.experimentsWidget.selectionModel())
self.handle_commit_button()
def UpdateResultsList(self, reload=False):
results_list = {}
try:
results_list = self.dbc.results_list(self.rtype(), reload=reload)
except Exception as ex:
try:
results_list = self.dbc.results_list(self.rtype())
except Exception as ex:
self.error(0, "Can not access database.")
self.results_list = results_list
mappings_key_dict = dict(((m["data_id"], m["id"]), key) \
for key, m in self.mappings.items())
def mapping_unique_id(annot):
"""Map annotations dict from results_list to unique
`mappings` ids.
"""
data_id, mappings_id = annot["data_id"], annot["mappings_id"]
return mappings_key_dict[data_id, mappings_id]
elements = []
# softly change the view so that the selection stays the same
items_shown = {}
for i, item in enumerate(self.items):
c = str(item.text(10))
items_shown[c] = i
items_to_show = dict((mapping_unique_id(annot), annot)
for annot in self.results_list.values())
add_items = set(items_to_show) - set(items_shown)
delete_items = set(items_shown) - set(items_to_show)
i = 0
while i < self.experimentsWidget.topLevelItemCount():
it = self.experimentsWidget.topLevelItem(i)
if str(it.text(10)) in delete_items:
self.experimentsWidget.takeTopLevelItem(i)
else:
i += 1
delete_ind = set([items_shown[i] for i in delete_items])
self.items = [it for i, it in enumerate(self.items) if i not in delete_ind]
for r_annot in [items_to_show[i] for i in add_items]:
d = defaultdict(lambda: "?", r_annot)
row_items = [""] + [d.get(key, "?") for key, _ in HEADER[1:]]
try:
time_dict = literal_eval(row_items[DATE_INDEX])
date_rna = date(time_dict["fullYearUTC"],
time_dict["monthUTC"] + 1, # Why is month 0 based?
time_dict["dateUTC"])
row_items[DATE_INDEX] = date_rna.strftime("%x")
except Exception:
row_items[DATE_INDEX] = ''
row_items[ID_INDEX] = mapping_unique_id(r_annot)
elements.append(row_items)
ci = MyTreeWidgetItem(self.experimentsWidget, row_items)
self.items.append(ci)
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
# which is the ok buffer version
# FIXME: what attribute to use for version?
self.wantbufver = \
lambda x, ad=self.results_list: \
defaultdict(lambda: "?", ad[x])["date"]
self.wantbufver = lambda x: "0"
self.UpdateCached()
def UpdateCached(self):
if self.wantbufver and self.dbc:
fn = self.dbc.download_key_function()
result_id_key = dict(((m["data_id"], m["mappings_id"]), key) \
for key, m in self.results_list.items())
for item in self.items:
c = str(item.text(10))
mapping = self.mappings[c]
data_id, mappings_id = mapping["data_id"], mapping["id"]
r_id = result_id_key[data_id, mappings_id]
# Get the buffered version
buffered = self.dbc.inBuffer(fn(r_id))
value = " " if buffered == self.wantbufver(r_id) else ""
item.setData(0, Qt.DisplayRole, value)
def SearchUpdate(self, string=""):
for item in self.items:
item.setHidden(not all(s in item \
for s in self.searchString.split())
)
def Commit(self):
if not self.dbc:
self.Connect()
pb = gui.ProgressBar(self, iterations=100)
table = None
ids = []
for item in self.experimentsWidget.selectedItems():
unique_id = str(item.text(10))
annots = self.mappings[unique_id]
ids.append((annots["data_id"], annots["id"]))
transfn = None
if self.log2:
transfn = lambda x: math.log(x + 1.0, 2)
reverse_header_dict = dict((name, key) for key, name in HEADER)
hview = self.experimentsWidget.header()
shownHeaders = [label for i, label in \
list(enumerate(self.headerLabels))[1:] \
if not hview.isSectionHidden(i)
]
allowed_labels = [reverse_header_dict.get(label, label) \
for label in shownHeaders]
if self.joinreplicates and "id" not in allowed_labels:
# need 'id' labels in join_replicates for attribute names
allowed_labels.append("id")
if len(ids):
table = self.dbc.get_data(ids=ids, result_type=self.rtype(),
callback=pb.advance,
exclude_constant_labels=self.excludeconstant,
# bufver=self.wantbufver,
transform=transfn,
allowed_labels=allowed_labels)
if self.joinreplicates:
table = dicty.join_replicates(table,
ignorenames=["replicate", "data_id", "mappings_id",
"data_name", "id", "unique_id"],
namefn=None,
avg=dicty.median
)
# Sort attributes
sortOrder = self.columnsSortingWidget.sortingOrder
all_values = defaultdict(set)
for at in table.domain.attributes:
atts = at.attributes
for name in sortOrder:
all_values[name].add(atts.get(reverse_header_dict[name], ""))
isnum = {}
for at, vals in all_values.items():
vals = filter(None, vals)
try:
for a in vals:
float(a)
isnum[at] = True
except:
isnum[at] = False
def optfloat(x, at):
if x == "":
return ""
else:
return float(x) if isnum[at] else x
def sorting_key(attr):
atts = attr.attributes
return tuple([optfloat(atts.get(reverse_header_dict[name], ""), name) \
for name in sortOrder])
attributes = sorted(table.domain.attributes,
key=sorting_key)
domain = Orange.data.Domain(
attributes, table.domain.class_var, table.domain.metas)
table = table.from_table(domain, table)
data_hints.set_hint(table, "taxid", "352472")
data_hints.set_hint(table, "genesinrows", False)
self.send("Data", table)
self.UpdateCached()
pb.finish()
def onSelectionChanged(self, selected, deselected):
self.handle_commit_button()
def handle_commit_button(self):
self.currentSelection = \
SelectionByKey(self.experimentsWidget.selectionModel().selection(),
key=(1, 2, 3, 10))
self.commit_button.setDisabled(not len(self.currentSelection))
def saveHeaderState(self):
hview = self.experimentsWidget.header()
for i, label in enumerate(self.headerLabels):
self.experimentsHeaderState[label] = hview.isSectionHidden(i)
def restoreHeaderState(self):
hview = self.experimentsWidget.header()
state = self.experimentsHeaderState
for i, label in enumerate(self.headerLabels):
hview.setSectionHidden(i, state.get(label, True))
self.experimentsWidget.resizeColumnToContents(i)
class OWGOBrowser(widget.OWWidget):
name = "GO Browser"
description = "Enrichment analysis for Gene Ontology terms."
icon = "../widgets/icons/OWGOBrowser.svg"
priority = 7
inputs = [("Cluster Data", Orange.data.Table,
"setDataset", widget.Single + widget.Default),
("Reference Data", Orange.data.Table,
"setReferenceDataset")]
outputs = [("Data on Selected Genes", Orange.data.Table),
("Enrichment Report", Orange.data.Table)]
settingsHandler = settings.DomainContextHandler()
geneAttrIndex = settings.ContextSetting(0)
useAttrNames = settings.ContextSetting(False)
useReferenceDataset = settings.Setting(False)
aspectIndex = settings.Setting(0)
useEvidenceType = settings.Setting(
{et: True for et in go.evidenceTypesOrdered})
filterByNumOfInstances = settings.Setting(False)
minNumOfInstances = settings.Setting(1)
filterByPValue = settings.Setting(True)
maxPValue = settings.Setting(0.2)
filterByPValue_nofdr = settings.Setting(False)
maxPValue_nofdr = settings.Setting(0.01)
probFunc = settings.Setting(0)
selectionDirectAnnotation = settings.Setting(0)
selectionDisjoint = settings.Setting(0)
class Error(widget.OWWidget.Error):
serverfiles_unavailable = widget.Msg('Can not locate annotation files, '
'please check your connection and try again.')
missing_annotation = widget.Msg(ERROR_ON_MISSING_ANNOTATION)
missing_gene_id = widget.Msg(ERROR_ON_MISSING_GENE_ID)
missing_tax_id = widget.Msg(ERROR_ON_MISSING_TAX_ID)
def __init__(self, parent=None):
super().__init__(self, parent)
self.input_data = None
self.ref_data = None
self.ontology = None
self.annotations = None
self.loaded_annotation_code = None
self.treeStructRootKey = None
self.probFunctions = [statistics.Binomial(), statistics.Hypergeometric()]
self.selectedTerms = []
self.selectionChanging = 0
self.__state = State.Ready
self.__scheduletimer = QTimer(self, singleShot=True)
self.__scheduletimer.timeout.connect(self.__update)
#############
# GUI
#############
self.tabs = gui.tabWidget(self.controlArea)
# Input tab
self.inputTab = gui.createTabPage(self.tabs, "Input")
box = gui.widgetBox(self.inputTab, "Info")
self.infoLabel = gui.widgetLabel(box, "No data on input\n")
gui.button(box, self, "Ontology/Annotation Info",
callback=self.ShowInfo,
tooltip="Show information on loaded ontology and annotations")
self.referenceRadioBox = gui.radioButtonsInBox(
self.inputTab, self, "useReferenceDataset",
["Entire genome", "Reference set (input)"],
tooltips=["Use entire genome for reference",
"Use genes from Referece Examples input signal as reference"],
box="Reference", callback=self.__invalidate)
self.referenceRadioBox.buttons[1].setDisabled(True)
gui.radioButtonsInBox(
self.inputTab, self, "aspectIndex",
["Biological process", "Cellular component", "Molecular function"],
box="Aspect", callback=self.__invalidate)
# Filter tab
self.filterTab = gui.createTabPage(self.tabs, "Filter")
box = gui.widgetBox(self.filterTab, "Filter GO Term Nodes")
gui.checkBox(box, self, "filterByNumOfInstances", "Genes",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by number of input genes mapped to a term")
ibox = gui.indentedBox(box)
gui.spin(ibox, self, 'minNumOfInstances', 1, 100,
step=1, label='#:', labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Min. number of input genes mapped to a term")
gui.checkBox(box, self, "filterByPValue_nofdr", "p-value",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by term p-value")
gui.doubleSpin(gui.indentedBox(box), self, 'maxPValue_nofdr', 1e-8, 1,
step=1e-8, label='p:', labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Max term p-value")
# use filterByPValue for FDR, as it was the default in prior versions
gui.checkBox(box, self, "filterByPValue", "FDR",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by term FDR")
gui.doubleSpin(gui.indentedBox(box), self, 'maxPValue', 1e-8, 1,
step=1e-8, label='p:', labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Max term p-value")
box = gui.widgetBox(box, "Significance test")
gui.radioButtonsInBox(box, self, "probFunc", ["Binomial", "Hypergeometric"],
tooltips=["Use binomial distribution test",
"Use hypergeometric distribution test"],
callback=self.__invalidate) # TODO: only update the p values
box = gui.widgetBox(self.filterTab, "Evidence codes in annotation",
addSpace=True)
self.evidenceCheckBoxDict = {}
for etype in go.evidenceTypesOrdered:
ecb = QCheckBox(
etype, toolTip=go.evidenceTypes[etype],
checked=self.useEvidenceType[etype])
ecb.toggled.connect(self.__on_evidenceChanged)
box.layout().addWidget(ecb)
self.evidenceCheckBoxDict[etype] = ecb
# Select tab
self.selectTab = gui.createTabPage(self.tabs, "Select")
box = gui.radioButtonsInBox(
self.selectTab, self, "selectionDirectAnnotation",
["Directly or Indirectly", "Directly"],
box="Annotated genes",
callback=self.ExampleSelection)
box = gui.widgetBox(self.selectTab, "Output", addSpace=True)
gui.radioButtonsInBox(
box, self, "selectionDisjoint",
btnLabels=["All selected genes",
"Term-specific genes",
"Common term genes"],
tooltips=["Outputs genes annotated to all selected GO terms",
"Outputs genes that appear in only one of selected GO terms",
"Outputs genes common to all selected GO terms"],
callback=self.ExampleSelection)
# ListView for DAG, and table for significant GOIDs
self.DAGcolumns = ['GO term', 'Cluster', 'Reference', 'p-value',
'FDR', 'Genes', 'Enrichment']
self.splitter = QSplitter(Qt.Vertical, self.mainArea)
self.mainArea.layout().addWidget(self.splitter)
# list view
self.listView = GOTreeWidget(self.splitter)
self.listView.setSelectionMode(QTreeView.ExtendedSelection)
self.listView.setAllColumnsShowFocus(1)
self.listView.setColumnCount(len(self.DAGcolumns))
self.listView.setHeaderLabels(self.DAGcolumns)
self.listView.header().setSectionsClickable(True)
self.listView.header().setSortIndicatorShown(True)
self.listView.header().setSortIndicator(self.DAGcolumns.index('p-value'), Qt.AscendingOrder)
self.listView.setSortingEnabled(True)
self.listView.setItemDelegateForColumn(
6, EnrichmentColumnItemDelegate(self))
self.listView.setRootIsDecorated(True)
self.listView.itemSelectionChanged.connect(self.ViewSelectionChanged)
# table of significant GO terms
self.sigTerms = QTreeWidget(self.splitter)
self.sigTerms.setColumnCount(len(self.DAGcolumns))
self.sigTerms.setHeaderLabels(self.DAGcolumns)
self.sigTerms.setSortingEnabled(True)
self.sigTerms.setSelectionMode(QTreeView.ExtendedSelection)
self.sigTerms.header().setSortIndicator(self.DAGcolumns.index('p-value'), Qt.AscendingOrder)
self.sigTerms.setItemDelegateForColumn(
6, EnrichmentColumnItemDelegate(self))
self.sigTerms.itemSelectionChanged.connect(self.TableSelectionChanged)
self.sigTableTermsSorted = []
self.graph = {}
self.originalGraph = None
self.inputTab.layout().addStretch(1)
self.filterTab.layout().addStretch(1)
self.selectTab.layout().addStretch(1)
class AnnotationSlot(SimpleNamespace):
taxid = ... # type: str
name = ... # type: str
filename = ... # type:str
@staticmethod
def parse_tax_id(f_name):
return f_name.split('.')[1]
try:
remote_files = serverfiles.ServerFiles().listfiles(DOMAIN)
except (ConnectTimeout, RequestException, ConnectionError):
# TODO: Warn user about failed connection to the remote server
remote_files = []
self.available_annotations = [
AnnotationSlot(
taxid=AnnotationSlot.parse_tax_id(annotation_file),
name=taxonomy.common_taxid_to_name(AnnotationSlot.parse_tax_id(annotation_file)),
filename=FILENAME_ANNOTATION.format(AnnotationSlot.parse_tax_id(annotation_file))
)
for _, annotation_file in set(remote_files + serverfiles.listfiles(DOMAIN))
if annotation_file != FILENAME_ONTOLOGY
]
self._executor = ThreadExecutor()
def sizeHint(self):
return QSize(1000, 700)
def __on_evidenceChanged(self):
for etype, cb in self.evidenceCheckBoxDict.items():
self.useEvidenceType[etype] = cb.isChecked()
self.__invalidate()
def clear(self):
self.infoLabel.setText("No data on input\n")
self.warning(0)
self.warning(1)
self.ClearGraph()
self.send("Data on Selected Genes", None)
self.send("Enrichment Report", None)
def setDataset(self, data=None):
self.closeContext()
self.clear()
self.Error.clear()
if data:
self.input_data = data
self.tax_id = str(self.input_data.attributes.get(TAX_ID, None))
self.use_attr_names = self.input_data.attributes.get(GENE_AS_ATTRIBUTE_NAME, None)
self.gene_id_attribute = self.input_data.attributes.get(GENE_ID_ATTRIBUTE, None)
self.gene_id_column = self.input_data.attributes.get(GENE_ID_COLUMN, None)
self.annotation_index = None
if not(self.use_attr_names is not None
and ((self.gene_id_attribute is None) ^ (self.gene_id_column is None))):
if self.tax_id is None:
self.Error.missing_annotation()
return
self.Error.missing_gene_id()
return
elif self.tax_id is None:
self.Error.missing_tax_id()
return
_c2i = {a.taxid: i for i, a in enumerate(self.available_annotations)}
try:
self.annotation_index = _c2i[self.tax_id]
except KeyError:
self.Error.serverfiles_unavailable()
# raise ValueError('Taxonomy {} not supported.'.format(self.tax_id))
return
self.__invalidate()
def setReferenceDataset(self, data=None):
self.Error.clear()
if data:
self.ref_data = data
self.ref_tax_id = str(self.ref_data.attributes.get(TAX_ID, None))
self.ref_use_attr_names = self.ref_data.attributes.get(GENE_AS_ATTRIBUTE_NAME, None)
self.ref_gene_id_attribute = self.ref_data.attributes.get(GENE_ID_ATTRIBUTE, None)
self.ref_gene_id_column = self.ref_data.attributes.get(GENE_ID_COLUMN, None)
if not (self.ref_use_attr_names is not None
and ((self.ref_gene_id_attribute is None) ^ (self.ref_gene_id_column is None))):
if self.ref_tax_id is None:
self.Error.missing_annotation()
return
self.Error.missing_gene_id()
return
elif self.ref_tax_id is None:
self.Error.missing_tax_id()
return
self.referenceRadioBox.buttons[1].setDisabled(not bool(data))
self.referenceRadioBox.buttons[1].setText("Reference set")
if self.input_data is not None and self.useReferenceDataset:
self.useReferenceDataset = 0 if not data else 1
self.__invalidate()
@Slot()
def __invalidate(self):
# Invalidate the current results or pending task and schedule an
# update.
self.__scheduletimer.start()
if self.__state != State.Ready:
self.__state |= State.Stale
self.SetGraph({})
self.ref_genes = None
self.input_genes = None
def __invalidateAnnotations(self):
self.annotations = None
self.loaded_annotation_code = None
if self.input_data:
self.infoLabel.setText("...\n")
self.__invalidate()
@Slot()
def __update(self):
self.__scheduletimer.stop()
if self.input_data is None:
return
if self.__state & State.Running:
self.__state |= State.Stale
elif self.__state & State.Downloading:
self.__state |= State.Stale
elif self.__state & State.Ready:
if self.__ensure_data():
self.Load()
self.Enrichment()
else:
assert self.__state & State.Downloading
assert self.isBlocking()
def __get_ref_genes(self):
self.ref_genes = []
if self.ref_use_attr_names:
for variable in self.input_data.domain.attributes:
self.ref_genes.append(str(variable.attributes.get(self.ref_gene_id_attribute, '?')))
else:
genes, _ = self.ref_data.get_column_view(self.ref_gene_id_column)
self.ref_genes = [str(g) for g in genes]
def __get_input_genes(self):
self.input_genes = []
if self.use_attr_names:
for variable in self.input_data.domain.attributes:
self.input_genes .append(str(variable.attributes.get(self.gene_id_attribute, '?')))
else:
genes, _ = self.input_data.get_column_view(self.gene_id_column)
self.input_genes = [str(g) for g in genes]
def FilterAnnotatedGenes(self, genes):
matchedgenes = self.annotations.get_gene_names_translator(genes).values()
return matchedgenes, [gene for gene in genes if gene not in matchedgenes]
def __start_download(self, files_list):
# type: (List[Tuple[str, str]]) -> None
task = EnsureDownloaded(files_list)
task.progress.connect(self._progressBarSet)
f = self._executor.submit(task)
fw = FutureWatcher(f, self)
fw.finished.connect(self.__download_finish)
fw.finished.connect(fw.deleteLater)
fw.resultReady.connect(self.__invalidate)
self.progressBarInit(processEvents=None)
self.setBlocking(True)
self.setStatusMessage("Downloading")
self.__state = State.Downloading
@Slot(Future)
def __download_finish(self, result):
# type: (Future[None]) -> None
assert QThread.currentThread() is self.thread()
assert result.done()
self.setBlocking(False)
self.setStatusMessage("")
self.progressBarFinished(processEvents=False)
try:
result.result()
except ConnectTimeout:
logging.getLogger(__name__).error("Error:")
self.error(2, "Internet connection error, unable to load data. " +
"Check connection and create a new GO Browser widget.")
except RequestException as err:
logging.getLogger(__name__).error("Error:")
self.error(2, "Internet error:\n" + str(err))
except BaseException as err:
logging.getLogger(__name__).error("Error:")
self.error(2, "Error:\n" + str(err))
raise
else:
self.error(2)
finally:
self.__state = State.Ready
def __ensure_data(self):
# Ensure that all required database (ontology and annotations for
# the current selected organism are present. If not start a download in
# the background. Return True if all dbs are present and false
# otherwise
assert self.__state == State.Ready
annotation = self.available_annotations[self.annotation_index]
go_files = [fname for domain, fname in serverfiles.listfiles(DOMAIN)]
files = []
if annotation.filename not in go_files:
files.append(("GO", annotation.filename))
if FILENAME_ONTOLOGY not in go_files:
files.append((DOMAIN, FILENAME_ONTOLOGY))
if files:
self.__start_download(files)
assert self.__state == State.Downloading
return False
else:
return True
def Load(self):
a = self.available_annotations[self.annotation_index]
if self.ontology is None:
self.ontology = go.Ontology()
if a.taxid != self.loaded_annotation_code:
self.annotations = None
gc.collect() # Force run garbage collection
self.annotations = go.Annotations(a.taxid)
self.loaded_annotation_code = a.taxid
count = defaultdict(int)
geneSets = defaultdict(set)
for anno in self.annotations.annotations:
count[anno.evidence] += 1
geneSets[anno.evidence].add(anno.gene_id)
for etype in go.evidenceTypesOrdered:
ecb = self.evidenceCheckBoxDict[etype]
ecb.setEnabled(bool(count[etype]))
ecb.setText(etype + ": %i annots(%i genes)" % (count[etype], len(geneSets[etype])))
def Enrichment(self):
assert self.input_data is not None
assert self.__state == State.Ready
if not self.annotations.ontology:
self.annotations.ontology = self.ontology
self.error(1)
self.warning([0, 1])
self.__get_input_genes()
self.input_genes = set(self.input_genes)
self.known_input_genes = self.annotations.get_genes_with_known_annotation(self.input_genes)
# self.clusterGenes = clusterGenes = self.annotations.map_to_ncbi_id(self.input_genes).values()
self.infoLabel.setText("%i unique genes on input\n%i (%.1f%%) genes with known annotations" %
(len(self.input_genes), len(self.known_input_genes),
100.0*len(self.known_input_genes)/len(self.input_genes)
if len(self.input_genes) else 0.0))
if not self.useReferenceDataset or self.ref_data is None:
self.information(2)
self.information(1)
self.ref_genes = self.annotations.genes()
self.ref_genes = set(self.ref_genes)
elif self.ref_data is not None:
self.__get_ref_genes()
self.ref_genes = set(self.ref_genes)
ref_count = len(self.ref_genes)
if ref_count == 0:
self.ref_genes = self.annotations.genes()
self.referenceRadioBox.buttons[1].setText("Reference set")
self.referenceRadioBox.buttons[1].setDisabled(True)
self.information(2, "Unable to extract gene names from reference dataset. "
"Using entire genome for reference")
self.useReferenceDataset = 0
else:
self.referenceRadioBox.buttons[1].setText("Reference set ({} genes)".format(ref_count))
self.referenceRadioBox.buttons[1].setDisabled(False)
self.information(2)
else:
self.useReferenceDataset = 0
self.ref_genes = []
if not self.ref_genes:
self.error(1, "No valid reference set")
return {}
evidences = []
for etype in go.evidenceTypesOrdered:
if self.useEvidenceType[etype]:
evidences.append(etype)
aspect = ['Process', 'Component', 'Function'][self.aspectIndex]
self.progressBarInit(processEvents=False)
self.setBlocking(True)
self.__state = State.Running
if self.input_genes:
f = self._executor.submit(
self.annotations.get_enriched_terms,
self.input_genes, self.ref_genes, evidences, aspect=aspect,
prob=self.probFunctions[self.probFunc], use_fdr=False,
progress_callback=methodinvoke(
self, "_progressBarSet", (float,))
)
fw = FutureWatcher(f, parent=self)
fw.done.connect(self.__on_enrichment_done)
fw.done.connect(fw.deleteLater)
return
else:
f = Future()
f.set_result({})
self.__on_enrichment_done(f)
def __on_enrichment_done(self, results):
# type: (Future[Dict[str, tuple]]) -> None
self.progressBarFinished(processEvents=False)
self.setBlocking(False)
self.setStatusMessage("")
if self.__state & State.Stale:
self.__state = State.Ready
self.__invalidate()
return
self.__state = State.Ready
try:
results = results.result() # type: Dict[str, tuple]
except Exception as ex:
results = {}
error = str(ex)
self.error(1, error)
if results:
terms = list(results.items())
fdr_vals = statistics.FDR([d[1] for _, d in terms])
terms = [(key, d + (fdr,))
for (key, d), fdr in zip(terms, fdr_vals)]
terms = dict(terms)
else:
terms = {}
self.terms = terms
if not self.terms:
self.warning(0, "No enriched terms found.")
else:
self.warning(0)
self.treeStructDict = {}
ids = self.terms.keys()
self.treeStructRootKey = None
parents = {}
for id in ids:
parents[id] = set([term for _, term in self.ontology[id].related])
children = {}
for term in self.terms:
children[term] = set([id for id in ids if term in parents[id]])
for term in self.terms:
self.treeStructDict[term] = TreeNode(self.terms[term], children[term])
if not self.ontology[term].related and not getattr(self.ontology[term], "is_obsolete", False):
self.treeStructRootKey = term
self.SetGraph(terms)
self._updateEnrichmentReportOutput()
self.commit()
def _updateEnrichmentReportOutput(self):
terms = sorted(self.terms.items(), key=lambda item: item[1][1])
# Create and send the enrichemnt report table.
termsDomain = Orange.data.Domain(
[], [],
# All is meta!
[Orange.data.StringVariable("GO Term Id"),
Orange.data.StringVariable("GO Term Name"),
Orange.data.ContinuousVariable("Cluster Frequency"),
Orange.data.ContinuousVariable("Genes in Cluster",
number_of_decimals=0),
Orange.data.ContinuousVariable("Reference Frequency"),
Orange.data.ContinuousVariable("Genes in Reference",
number_of_decimals=0),
Orange.data.ContinuousVariable("p-value"),
Orange.data.ContinuousVariable("FDR"),
Orange.data.ContinuousVariable("Enrichment"),
Orange.data.StringVariable("Genes")])
terms = [[t_id,
self.ontology[t_id].name,
len(genes) / len(self.input_genes),
len(genes),
r_count / len(self.ref_genes),
r_count,
p_value,
fdr,
len(genes) / len(self.input_genes) * \
len(self.ref_genes) / r_count,
",".join(genes)
]
for t_id, (genes, p_value, r_count, fdr) in terms
if genes and r_count]
if terms:
X = numpy.empty((len(terms), 0))
M = numpy.array(terms, dtype=object)
termsTable = Orange.data.Table.from_numpy(termsDomain, X,
metas=M)
else:
termsTable = None
self.send("Enrichment Report", termsTable)
@Slot(float)
def _progressBarSet(self, value):
assert QThread.currentThread() is self.thread()
self.progressBarSet(value, processEvents=None)
@Slot()
def _progressBarFinish(self):
assert QThread.currentThread() is self.thread()
self.progressBarFinished(processEvents=None)
def FilterGraph(self, graph):
if self.filterByPValue_nofdr:
graph = go.filterByPValue(graph, self.maxPValue_nofdr)
if self.filterByPValue: # FDR
graph = dict(filter(lambda item: item[1][3] <= self.maxPValue, graph.items()))
if self.filterByNumOfInstances:
graph = dict(filter(lambda item: len(item[1][0]) >= self.minNumOfInstances, graph.items()))
return graph
def FilterAndDisplayGraph(self):
if self.input_data and self.originalGraph is not None:
self.graph = self.FilterGraph(self.originalGraph)
if self.originalGraph and not self.graph:
self.warning(1, "All found terms were filtered out.")
else:
self.warning(1)
self.ClearGraph()
self.DisplayGraph()
def SetGraph(self, graph=None):
self.originalGraph = graph
if graph:
self.FilterAndDisplayGraph()
else:
self.graph = {}
self.ClearGraph()
def ClearGraph(self):
self.listView.clear()
self.listViewItems=[]
self.sigTerms.clear()
def DisplayGraph(self):
fromParentDict = {}
self.termListViewItemDict = {}
self.listViewItems = []
def enrichment(t):
try:
return len(t[0]) / t[2] * (len(self.ref_genes) / len(self.input_genes))
except ZeroDivisionError:
# TODO: find out why this happens
return 0
maxFoldEnrichment = max([enrichment(term) for term in self.graph.values()] or [1])
def addNode(term, parent, parentDisplayNode):
if (parent, term) in fromParentDict:
return
if term in self.graph:
displayNode = GOTreeWidgetItem(self.ontology[term], self.graph[term], len(self.input_genes),
len(self.ref_genes), maxFoldEnrichment, parentDisplayNode)
displayNode.goId = term
self.listViewItems.append(displayNode)
if term in self.termListViewItemDict:
self.termListViewItemDict[term].append(displayNode)
else:
self.termListViewItemDict[term] = [displayNode]
fromParentDict[(parent, term)] = True
parent = term
else:
displayNode = parentDisplayNode
for c in self.treeStructDict[term].children:
addNode(c, parent, displayNode)
if self.treeStructDict:
addNode(self.treeStructRootKey, None, self.listView)
terms = self.graph.items()
terms = sorted(terms, key=lambda item: item[1][1])
self.sigTableTermsSorted = [t[0] for t in terms]
self.sigTerms.clear()
for i, (t_id, (genes, p_value, refCount, fdr)) in enumerate(terms):
item = GOTreeWidgetItem(self.ontology[t_id],
(genes, p_value, refCount, fdr),
len(self.input_genes),
len(self.ref_genes),
maxFoldEnrichment,
self.sigTerms)
item.goId = t_id
self.listView.expandAll()
for i in range(5):
self.listView.resizeColumnToContents(i)
self.sigTerms.resizeColumnToContents(i)
self.sigTerms.resizeColumnToContents(6)
width = min(self.listView.columnWidth(0), 350)
self.listView.setColumnWidth(0, width)
self.sigTerms.setColumnWidth(0, width)
def ViewSelectionChanged(self):
if self.selectionChanging:
return
self.selectionChanging = 1
self.selectedTerms = []
selected = self.listView.selectedItems()
self.selectedTerms = list(set([lvi.term.id for lvi in selected]))
self.ExampleSelection()
self.selectionChanging = 0
def TableSelectionChanged(self):
if self.selectionChanging:
return
self.selectionChanging = 1
self.selectedTerms = []
selectedIds = set([self.sigTerms.itemFromIndex(index).goId for index in self.sigTerms.selectedIndexes()])
for i in range(self.sigTerms.topLevelItemCount()):
item = self.sigTerms.topLevelItem(i)
selected = item.goId in selectedIds
term = item.goId
if selected:
self.selectedTerms.append(term)
for lvi in self.termListViewItemDict[term]:
try:
lvi.setSelected(selected)
if selected:
lvi.setExpanded(True)
except RuntimeError: # Underlying C/C++ object deleted
pass
self.selectionChanging = 0
self.ExampleSelection()
def ExampleSelection(self):
self.commit()
def commit(self):
if self.input_data is None or self.originalGraph is None or \
self.annotations is None:
return
if self.__state & State.Stale:
return
terms = set(self.selectedTerms)
genes = reduce(operator.ior,
(set(self.graph[term][0]) for term in terms), set())
evidences = []
for etype in go.evidenceTypesOrdered:
if self.useEvidenceType[etype]:
evidences.append(etype)
allTerms = self.annotations.get_annotated_terms(
genes, direct_annotation_only=self.selectionDirectAnnotation,
evidence_codes=evidences)
if self.selectionDisjoint > 0:
count = defaultdict(int)
for term in self.selectedTerms:
for g in allTerms.get(term, []):
count[g] += 1
ccount = 1 if self.selectionDisjoint == 1 else len(self.selectedTerms)
selected_genes = [gene for gene, c in count.items() if c == ccount and gene in genes]
else:
selected_genes = reduce(
operator.ior,
(set(allTerms.get(term, [])) for term in self.selectedTerms), set())
if self.use_attr_names:
selected = [column for column in self.input_data.domain.attributes
if self.gene_id_attribute in column.attributes and
str(column.attributes[self.gene_id_attribute]) in set(selected_genes)]
domain = Orange.data.Domain(selected, self.input_data.domain.class_vars, self.input_data.domain.metas)
new_data = self.input_data.from_table(domain, self.input_data)
self.send("Data on Selected Genes", new_data)
else:
selected_rows = []
for row_index, row in enumerate(self.input_data):
gene_in_row = str(row[self.gene_id_column])
if gene_in_row in self.input_genes and gene_in_row in selected_genes:
selected_rows.append(row_index)
if selected_rows:
selected = self.input_data[selected_rows]
else:
selected = None
self.send("Data on Selected Genes", selected)
def ShowInfo(self):
dialog = QDialog(self)
dialog.setModal(False)
dialog.setLayout(QVBoxLayout())
label = QLabel(dialog)
label.setText("Ontology:\n" + self.ontology.header
if self.ontology else "Ontology not loaded!")
dialog.layout().addWidget(label)
label = QLabel(dialog)
label.setText("Annotations:\n" + self.annotations.header.replace("!", "")
if self.annotations else "Annotations not loaded!")
dialog.layout().addWidget(label)
dialog.show()
def onDeleteWidget(self):
"""Called before the widget is removed from the canvas.
"""
self.annotations = None
self.ontology = None
gc.collect() # Force collection
class OWGOEnrichmentAnalysis(widget.OWWidget):
name = "GO Browser"
description = "Enrichment analysis for Gene Ontology terms."
icon = "../widgets/icons/GOBrowser.svg"
priority = 2020
inputs = [("Cluster Data", Orange.data.Table, "setDataset",
widget.Single + widget.Default),
("Reference Data", Orange.data.Table, "setReferenceDataset")]
outputs = [("Data on Selected Genes", Orange.data.Table),
("Data on Unselected Genes", Orange.data.Table),
("Data on Unknown Genes", Orange.data.Table),
("Enrichment Report", Orange.data.Table)]
settingsHandler = settings.DomainContextHandler()
annotationIndex = settings.ContextSetting(0)
geneAttrIndex = settings.ContextSetting(0)
useAttrNames = settings.ContextSetting(False)
geneMatcherSettings = settings.Setting([True, False, False, False])
useReferenceDataset = settings.Setting(False)
aspectIndex = settings.Setting(0)
useEvidenceType = settings.Setting(
{et: True
for et in go.evidenceTypesOrdered})
filterByNumOfInstances = settings.Setting(False)
minNumOfInstances = settings.Setting(1)
filterByPValue = settings.Setting(True)
maxPValue = settings.Setting(0.2)
filterByPValue_nofdr = settings.Setting(False)
maxPValue_nofdr = settings.Setting(0.01)
probFunc = settings.Setting(0)
selectionDirectAnnotation = settings.Setting(0)
selectionDisjoint = settings.Setting(0)
selectionAddTermAsClass = settings.Setting(0)
Ready, Initializing, Running = 0, 1, 2
def __init__(self, parent=None):
super().__init__(self, parent)
self.clusterDataset = None
self.referenceDataset = None
self.ontology = None
self.annotations = None
self.loadedAnnotationCode = "---"
self.treeStructRootKey = None
self.probFunctions = [stats.Binomial(), stats.Hypergeometric()]
self.selectedTerms = []
self.selectionChanging = 0
self.__state = OWGOEnrichmentAnalysis.Initializing
self.annotationCodes = []
#############
## GUI
#############
self.tabs = gui.tabWidget(self.controlArea)
## Input tab
self.inputTab = gui.createTabPage(self.tabs, "Input")
box = gui.widgetBox(self.inputTab, "Info")
self.infoLabel = gui.widgetLabel(box, "No data on input\n")
gui.button(
box,
self,
"Ontology/Annotation Info",
callback=self.ShowInfo,
tooltip="Show information on loaded ontology and annotations")
box = gui.widgetBox(self.inputTab, "Organism")
self.annotationComboBox = gui.comboBox(box,
self,
"annotationIndex",
items=self.annotationCodes,
callback=self._updateEnrichment,
tooltip="Select organism")
genebox = gui.widgetBox(self.inputTab, "Gene Names")
self.geneAttrIndexCombo = gui.comboBox(
genebox,
self,
"geneAttrIndex",
callback=self._updateEnrichment,
tooltip="Use this attribute to extract gene names from input data")
self.geneAttrIndexCombo.setDisabled(self.useAttrNames)
cb = gui.checkBox(genebox,
self,
"useAttrNames",
"Use column names",
tooltip="Use column names for gene names",
callback=self._updateEnrichment)
cb.toggled[bool].connect(self.geneAttrIndexCombo.setDisabled)
gui.button(genebox,
self,
"Gene matcher settings",
callback=self.UpdateGeneMatcher,
tooltip="Open gene matching settings dialog")
self.referenceRadioBox = gui.radioButtonsInBox(
self.inputTab,
self,
"useReferenceDataset", ["Entire genome", "Reference set (input)"],
tooltips=[
"Use entire genome for reference",
"Use genes from Referece Examples input signal as reference"
],
box="Reference",
callback=self._updateEnrichment)
self.referenceRadioBox.buttons[1].setDisabled(True)
gui.radioButtonsInBox(
self.inputTab,
self,
"aspectIndex",
["Biological process", "Cellular component", "Molecular function"],
box="Aspect",
callback=self._updateEnrichment)
## Filter tab
self.filterTab = gui.createTabPage(self.tabs, "Filter")
box = gui.widgetBox(self.filterTab, "Filter GO Term Nodes")
gui.checkBox(
box,
self,
"filterByNumOfInstances",
"Genes",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by number of input genes mapped to a term")
ibox = gui.indentedBox(box)
gui.spin(ibox,
self,
'minNumOfInstances',
1,
100,
step=1,
label='#:',
labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Min. number of input genes mapped to a term")
gui.checkBox(box,
self,
"filterByPValue_nofdr",
"p-value",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by term p-value")
gui.doubleSpin(gui.indentedBox(box),
self,
'maxPValue_nofdr',
1e-8,
1,
step=1e-8,
label='p:',
labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Max term p-value")
#use filterByPValue for FDR, as it was the default in prior versions
gui.checkBox(box,
self,
"filterByPValue",
"FDR",
callback=self.FilterAndDisplayGraph,
tooltip="Filter by term FDR")
gui.doubleSpin(gui.indentedBox(box),
self,
'maxPValue',
1e-8,
1,
step=1e-8,
label='p:',
labelWidth=15,
callback=self.FilterAndDisplayGraph,
callbackOnReturn=True,
tooltip="Max term p-value")
box = gui.widgetBox(box, "Significance test")
gui.radioButtonsInBox(box,
self,
"probFunc", ["Binomial", "Hypergeometric"],
tooltips=[
"Use binomial distribution test",
"Use hypergeometric distribution test"
],
callback=self._updateEnrichment)
box = gui.widgetBox(self.filterTab,
"Evidence codes in annotation",
addSpace=True)
self.evidenceCheckBoxDict = {}
for etype in go.evidenceTypesOrdered:
ecb = QCheckBox(etype,
toolTip=go.evidenceTypes[etype],
checked=self.useEvidenceType[etype])
ecb.toggled.connect(self.__on_evidenceChanged)
box.layout().addWidget(ecb)
self.evidenceCheckBoxDict[etype] = ecb
## Select tab
self.selectTab = gui.createTabPage(self.tabs, "Select")
box = gui.radioButtonsInBox(self.selectTab,
self,
"selectionDirectAnnotation",
["Directly or Indirectly", "Directly"],
box="Annotated genes",
callback=self.ExampleSelection)
box = gui.widgetBox(self.selectTab, "Output", addSpace=True)
gui.radioButtonsInBox(
box,
self,
"selectionDisjoint",
btnLabels=[
"All selected genes", "Term-specific genes",
"Common term genes"
],
tooltips=[
"Outputs genes annotated to all selected GO terms",
"Outputs genes that appear in only one of selected GO terms",
"Outputs genes common to all selected GO terms"
],
callback=[self.ExampleSelection, self.UpdateAddClassButton])
self.addClassCB = gui.checkBox(box,
self,
"selectionAddTermAsClass",
"Add GO Term as class",
callback=self.ExampleSelection)
# ListView for DAG, and table for significant GOIDs
self.DAGcolumns = [
'GO term', 'Cluster', 'Reference', 'p-value', 'FDR', 'Genes',
'Enrichment'
]
self.splitter = QSplitter(Qt.Vertical, self.mainArea)
self.mainArea.layout().addWidget(self.splitter)
# list view
self.listView = GOTreeWidget(self.splitter)
self.listView.setSelectionMode(QTreeView.ExtendedSelection)
self.listView.setAllColumnsShowFocus(1)
self.listView.setColumnCount(len(self.DAGcolumns))
self.listView.setHeaderLabels(self.DAGcolumns)
self.listView.header().setSectionsClickable(True)
self.listView.header().setSortIndicatorShown(True)
self.listView.setSortingEnabled(True)
self.listView.setItemDelegateForColumn(
6, EnrichmentColumnItemDelegate(self))
self.listView.setRootIsDecorated(True)
self.listView.itemSelectionChanged.connect(self.ViewSelectionChanged)
# table of significant GO terms
self.sigTerms = QTreeWidget(self.splitter)
self.sigTerms.setColumnCount(len(self.DAGcolumns))
self.sigTerms.setHeaderLabels(self.DAGcolumns)
self.sigTerms.setSortingEnabled(True)
self.sigTerms.setSelectionMode(QTreeView.ExtendedSelection)
self.sigTerms.setItemDelegateForColumn(
6, EnrichmentColumnItemDelegate(self))
self.sigTerms.itemSelectionChanged.connect(self.TableSelectionChanged)
self.sigTableTermsSorted = []
self.graph = {}
self.inputTab.layout().addStretch(1)
self.filterTab.layout().addStretch(1)
self.selectTab.layout().addStretch(1)
self.setBlocking(True)
self._executor = ThreadExecutor()
self._init = EnsureDownloaded([(taxonomy.Taxonomy.DOMAIN,
taxonomy.Taxonomy.FILENAME),
("GO", "taxonomy.pickle")])
self._init.finished.connect(self.__initialize_finish)
self._executor.submit(self._init)
def sizeHint(self):
return QSize(1000, 700)
def __initialize_finish(self):
self.setBlocking(False)
try:
self.annotationFiles = listAvailable()
except ConnectTimeout:
self.error(2, "Internet connection error, unable to load data. " + \
"Check connection and create a new GO Browser widget.")
self.filterTab.setEnabled(False)
self.inputTab.setEnabled(False)
self.selectTab.setEnabled(False)
self.listView.setEnabled(False)
self.sigTerms.setEnabled(False)
else:
self.annotationCodes = sorted(self.annotationFiles.keys())
self.annotationComboBox.clear()
self.annotationComboBox.addItems(self.annotationCodes)
self.annotationComboBox.setCurrentIndex(self.annotationIndex)
self.__state = OWGOEnrichmentAnalysis.Ready
def __on_evidenceChanged(self):
for etype, cb in self.evidenceCheckBoxDict.items():
self.useEvidenceType[etype] = cb.isChecked()
self._updateEnrichment()
def UpdateGeneMatcher(self):
"""Open the Gene matcher settings dialog."""
dialog = GeneMatcherDialog(self,
defaults=self.geneMatcherSettings,
modal=True)
if dialog.exec_() != QDialog.Rejected:
self.geneMatcherSettings = [
getattr(dialog, item[0]) for item in dialog.items
]
if self.annotations:
self.SetGeneMatcher()
self._updateEnrichment()
def clear(self):
self.infoLabel.setText("No data on input\n")
self.warning(0)
self.warning(1)
self.geneAttrIndexCombo.clear()
self.ClearGraph()
self.send("Data on Selected Genes", None)
self.send("Data on Unselected Genes", None)
self.send("Data on Unknown Genes", None)
self.send("Enrichment Report", None)
def setDataset(self, data=None):
if self.__state == OWGOEnrichmentAnalysis.Initializing:
self.__initialize_finish()
self.closeContext()
self.clear()
self.clusterDataset = data
if data is not None:
domain = data.domain
allvars = domain.variables + domain.metas
self.candidateGeneAttrs = [var for var in allvars if isstring(var)]
self.geneAttrIndexCombo.clear()
for var in self.candidateGeneAttrs:
self.geneAttrIndexCombo.addItem(*gui.attributeItem(var))
taxid = data_hints.get_hint(data, "taxid", "")
code = None
try:
code = go.from_taxid(taxid)
except KeyError:
pass
except Exception as ex:
print(ex)
if code is not None:
filename = "gene_association.%s.tar.gz" % code
if filename in self.annotationFiles.values():
self.annotationIndex = \
[i for i, name in enumerate(self.annotationCodes) \
if self.annotationFiles[name] == filename].pop()
self.useAttrNames = data_hints.get_hint(data, "genesinrows",
self.useAttrNames)
self.openContext(data)
self.geneAttrIndex = min(self.geneAttrIndex,
len(self.candidateGeneAttrs) - 1)
if len(self.candidateGeneAttrs) == 0:
self.useAttrNames = True
self.geneAttrIndex = -1
elif self.geneAttrIndex < len(self.candidateGeneAttrs):
self.geneAttrIndex = len(self.candidateGeneAttrs) - 1
self._updateEnrichment()
def setReferenceDataset(self, data=None):
self.referenceDataset = data
self.referenceRadioBox.buttons[1].setDisabled(not bool(data))
self.referenceRadioBox.buttons[1].setText("Reference set")
if self.clusterDataset is not None and self.useReferenceDataset:
self.useReferenceDataset = 0 if not data else 1
graph = self.Enrichment()
self.SetGraph(graph)
elif self.clusterDataset:
self.__updateReferenceSetButton()
def handleNewSignals(self):
super().handleNewSignals()
def _updateEnrichment(self):
if self.clusterDataset is not None and \
self.__state == OWGOEnrichmentAnalysis.Ready:
pb = gui.ProgressBar(self, 100)
self.Load(pb=pb)
graph = self.Enrichment(pb=pb)
self.FilterUnknownGenes()
self.SetGraph(graph)
def __updateReferenceSetButton(self):
allgenes, refgenes = None, None
if self.referenceDataset:
try:
allgenes = self.genesFromTable(self.referenceDataset)
except Exception:
allgenes = []
refgenes, unknown = self.FilterAnnotatedGenes(allgenes)
self.referenceRadioBox.buttons[1].setDisabled(not bool(allgenes))
self.referenceRadioBox.buttons[1].setText("Reference set " + (
"(%i genes, %i matched)" %
(len(allgenes), len(refgenes)) if allgenes and refgenes else ""))
def genesFromTable(self, data):
if self.useAttrNames:
genes = [v.name for v in data.domain.variables]
else:
attr = self.candidateGeneAttrs[min(
self.geneAttrIndex,
len(self.candidateGeneAttrs) - 1)]
genes = [str(ex[attr]) for ex in data if not numpy.isnan(ex[attr])]
if any("," in gene for gene in genes):
self.information(
0,
"Separators detected in gene names. Assuming multiple genes per example."
)
genes = reduce(operator.iadd,
(genes.split(",") for genes in genes), [])
return genes
def FilterAnnotatedGenes(self, genes):
matchedgenes = self.annotations.get_gene_names_translator(
genes).values()
return matchedgenes, [
gene for gene in genes if gene not in matchedgenes
]
def FilterUnknownGenes(self):
if not self.useAttrNames and self.candidateGeneAttrs:
geneAttr = self.candidateGeneAttrs[min(
self.geneAttrIndex,
len(self.candidateGeneAttrs) - 1)]
indices = []
for i, ex in enumerate(self.clusterDataset):
if not any(
self.annotations.genematcher.match(n.strip())
for n in str(ex[geneAttr]).split(",")):
indices.append(i)
if indices:
data = self.clusterDataset[indices]
else:
data = None
self.send("Data on Unknown Genes", data)
else:
self.send("Data on Unknown Genes", None)
def Load(self, pb=None):
if self.__state == OWGOEnrichmentAnalysis.Ready:
go_files, tax_files = serverfiles.listfiles(
"GO"), serverfiles.listfiles("Taxonomy")
calls = []
pb, finish = (gui.ProgressBar(self, 0),
True) if pb is None else (pb, False)
count = 0
if not tax_files:
calls.append(("Taxonomy", "ncbi_taxnomy.tar.gz"))
count += 1
org = self.annotationCodes[min(self.annotationIndex,
len(self.annotationCodes) - 1)]
if org != self.loadedAnnotationCode:
count += 1
if self.annotationFiles[org] not in go_files:
calls.append(("GO", self.annotationFiles[org]))
count += 1
if "gene_ontology_edit.obo.tar.gz" not in go_files:
calls.append(("GO", "gene_ontology_edit.obo.tar.gz"))
count += 1
if not self.ontology:
count += 1
pb.iter += count * 100
for args in calls:
serverfiles.localpath_download(*args,
**dict(callback=pb.advance))
i = len(calls)
if not self.ontology:
self.ontology = go.Ontology(
progress_callback=lambda value: pb.advance())
i += 1
if org != self.loadedAnnotationCode:
self.annotations = None
gc.collect() # Force run garbage collection
code = self.annotationFiles[org].split(".")[-3]
self.annotations = go.Annotations(
code,
genematcher=gene.GMDirect(),
progress_callback=lambda value: pb.advance())
i += 1
self.loadedAnnotationCode = org
count = defaultdict(int)
geneSets = defaultdict(set)
for anno in self.annotations.annotations:
count[anno.evidence] += 1
geneSets[anno.evidence].add(anno.geneName)
for etype in go.evidenceTypesOrdered:
ecb = self.evidenceCheckBoxDict[etype]
ecb.setEnabled(bool(count[etype]))
ecb.setText(etype + ": %i annots(%i genes)" %
(count[etype], len(geneSets[etype])))
if finish:
pb.finish()
def SetGeneMatcher(self):
if self.annotations:
taxid = self.annotations.taxid
matchers = []
for matcher, use in zip(
[gene.GMGO, gene.GMKEGG, gene.GMNCBI, gene.GMAffy],
self.geneMatcherSettings):
if use:
try:
if taxid == "352472":
matchers.extend([
matcher(taxid),
gene.GMDicty(),
[matcher(taxid),
gene.GMDicty()]
])
# The reason machers are duplicated is that we want `matcher` or `GMDicty` to
# match genes by them self if possible. Only use the joint matcher if they fail.
else:
matchers.append(matcher(taxid))
except Exception as ex:
print(ex)
self.annotations.genematcher = gene.matcher(matchers)
self.annotations.genematcher.set_targets(
self.annotations.gene_names)
def Enrichment(self, pb=None):
assert self.clusterDataset is not None
pb = gui.ProgressBar(self, 100) if pb is None else pb
if not self.annotations.ontology:
self.annotations.ontology = self.ontology
if isinstance(self.annotations.genematcher, gene.GMDirect):
self.SetGeneMatcher()
self.error(1)
self.warning([0, 1])
if self.useAttrNames:
clusterGenes = [
v.name for v in self.clusterDataset.domain.attributes
]
self.information(0)
elif 0 <= self.geneAttrIndex < len(self.candidateGeneAttrs):
geneAttr = self.candidateGeneAttrs[self.geneAttrIndex]
clusterGenes = [
str(ex[geneAttr]) for ex in self.clusterDataset
if not numpy.isnan(ex[geneAttr])
]
if any("," in gene for gene in clusterGenes):
self.information(
0,
"Separators detected in cluster gene names. Assuming multiple genes per example."
)
clusterGenes = reduce(operator.iadd,
(genes.split(",")
for genes in clusterGenes), [])
else:
self.information(0)
else:
self.error(1, "Failed to extract gene names from input dataset!")
return {}
genesSetCount = len(set(clusterGenes))
self.clusterGenes = clusterGenes = self.annotations.get_gene_names_translator(
clusterGenes).values()
self.infoLabel.setText(
"%i unique genes on input\n%i (%.1f%%) genes with known annotations"
% (genesSetCount, len(clusterGenes), 100.0 * len(clusterGenes) /
genesSetCount if genesSetCount else 0.0))
referenceGenes = None
if not self.useReferenceDataset or self.referenceDataset is None:
self.information(2)
self.information(1)
referenceGenes = self.annotations.gene_names
elif self.referenceDataset is not None:
if self.useAttrNames:
referenceGenes = [
v.name for v in self.referenceDataset.domain.attributes
]
self.information(1)
elif geneAttr in (self.referenceDataset.domain.variables +
self.referenceDataset.domain.metas):
referenceGenes = [
str(ex[geneAttr]) for ex in self.referenceDataset
if not numpy.isnan(ex[geneAttr])
]
if any("," in gene for gene in clusterGenes):
self.information(
1,
"Separators detected in reference gene names. Assuming multiple genes per example."
)
referenceGenes = reduce(operator.iadd,
(genes.split(",")
for genes in referenceGenes), [])
else:
self.information(1)
else:
self.information(1)
referenceGenes = None
if referenceGenes is None:
referenceGenes = list(self.annotations.gene_names)
self.referenceRadioBox.buttons[1].setText("Reference set")
self.referenceRadioBox.buttons[1].setDisabled(True)
self.information(
2,
"Unable to extract gene names from reference dataset. Using entire genome for reference"
)
self.useReferenceDataset = 0
else:
refc = len(referenceGenes)
referenceGenes = self.annotations.get_gene_names_translator(
referenceGenes).values()
self.referenceRadioBox.buttons[1].setText(
"Reference set (%i genes, %i matched)" %
(refc, len(referenceGenes)))
self.referenceRadioBox.buttons[1].setDisabled(False)
self.information(2)
else:
self.useReferenceDataset = 0
if not referenceGenes:
self.error(1, "No valid reference set")
return {}
self.referenceGenes = referenceGenes
evidences = []
for etype in go.evidenceTypesOrdered:
if self.useEvidenceType[etype]:
evidences.append(etype)
aspect = ["P", "C", "F"][self.aspectIndex]
if clusterGenes:
self.terms = terms = self.annotations.get_enriched_terms(
clusterGenes,
referenceGenes,
evidences,
aspect=aspect,
prob=self.probFunctions[self.probFunc],
use_fdr=False,
progress_callback=lambda value: pb.advance())
ids = []
pvals = []
for i, d in self.terms.items():
ids.append(i)
pvals.append(d[1])
for i, fdr in zip(ids, stats.FDR(
pvals)): # save FDR as the last part of the tuple
terms[i] = tuple(list(terms[i]) + [fdr])
else:
self.terms = terms = {}
if not self.terms:
self.warning(0, "No enriched terms found.")
else:
self.warning(0)
pb.finish()
self.treeStructDict = {}
ids = self.terms.keys()
self.treeStructRootKey = None
parents = {}
for id in ids:
parents[id] = set([term for _, term in self.ontology[id].related])
children = {}
for term in self.terms:
children[term] = set([id for id in ids if term in parents[id]])
for term in self.terms:
self.treeStructDict[term] = TreeNode(self.terms[term],
children[term])
if not self.ontology[term].related and not getattr(
self.ontology[term], "is_obsolete", False):
self.treeStructRootKey = term
return terms
def FilterGraph(self, graph):
if self.filterByPValue_nofdr:
graph = go.filterByPValue(graph, self.maxPValue_nofdr)
if self.filterByPValue: #FDR
graph = dict(
filter(lambda item: item[1][3] <= self.maxPValue,
graph.items()))
if self.filterByNumOfInstances:
graph = dict(
filter(lambda item: len(item[1][0]) >= self.minNumOfInstances,
graph.items()))
return graph
def FilterAndDisplayGraph(self):
if self.clusterDataset:
self.graph = self.FilterGraph(self.originalGraph)
if self.originalGraph and not self.graph:
self.warning(1, "All found terms were filtered out.")
else:
self.warning(1)
self.ClearGraph()
self.DisplayGraph()
def SetGraph(self, graph=None):
self.originalGraph = graph
if graph:
self.FilterAndDisplayGraph()
else:
self.graph = {}
self.ClearGraph()
def ClearGraph(self):
self.listView.clear()
self.listViewItems = []
self.sigTerms.clear()
def DisplayGraph(self):
fromParentDict = {}
self.termListViewItemDict = {}
self.listViewItems = []
enrichment = lambda t: len(t[0]) / t[2] * (len(self.referenceGenes) /
len(self.clusterGenes))
maxFoldEnrichment = max(
[enrichment(term) for term in self.graph.values()] or [1])
def addNode(term, parent, parentDisplayNode):
if (parent, term) in fromParentDict:
return
if term in self.graph:
displayNode = GOTreeWidgetItem(self.ontology[term],
self.graph[term],
len(self.clusterGenes),
len(self.referenceGenes),
maxFoldEnrichment,
parentDisplayNode)
displayNode.goId = term
self.listViewItems.append(displayNode)
if term in self.termListViewItemDict:
self.termListViewItemDict[term].append(displayNode)
else:
self.termListViewItemDict[term] = [displayNode]
fromParentDict[(parent, term)] = True
parent = term
else:
displayNode = parentDisplayNode
for c in self.treeStructDict[term].children:
addNode(c, parent, displayNode)
if self.treeStructDict:
addNode(self.treeStructRootKey, None, self.listView)
terms = self.graph.items()
terms = sorted(terms, key=lambda item: item[1][1])
self.sigTableTermsSorted = [t[0] for t in terms]
self.sigTerms.clear()
for i, (t_id, (genes, p_value, refCount, fdr)) in enumerate(terms):
item = GOTreeWidgetItem(self.ontology[t_id],
(genes, p_value, refCount, fdr),
len(self.clusterGenes),
len(self.referenceGenes),
maxFoldEnrichment, self.sigTerms)
item.goId = t_id
self.listView.expandAll()
for i in range(5):
self.listView.resizeColumnToContents(i)
self.sigTerms.resizeColumnToContents(i)
self.sigTerms.resizeColumnToContents(6)
width = min(self.listView.columnWidth(0), 350)
self.listView.setColumnWidth(0, width)
self.sigTerms.setColumnWidth(0, width)
# Create and send the enrichemnt report table.
termsDomain = Orange.data.Domain(
[],
[],
# All is meta!
[
Orange.data.StringVariable("GO Term Id"),
Orange.data.StringVariable("GO Term Name"),
Orange.data.ContinuousVariable("Cluster Frequency"),
Orange.data.ContinuousVariable("Genes in Cluster",
number_of_decimals=0),
Orange.data.ContinuousVariable("Reference Frequency"),
Orange.data.ContinuousVariable("Genes in Reference",
number_of_decimals=0),
Orange.data.ContinuousVariable("p-value"),
Orange.data.ContinuousVariable("FDR"),
Orange.data.ContinuousVariable("Enrichment"),
Orange.data.StringVariable("Genes")
])
terms = [[t_id,
self.ontology[t_id].name,
len(genes) / len(self.clusterGenes),
len(genes),
r_count / len(self.referenceGenes),
r_count,
p_value,
fdr,
len(genes) / len(self.clusterGenes) * \
len(self.referenceGenes) / r_count,
",".join(genes)
]
for t_id, (genes, p_value, r_count, fdr) in terms]
if terms:
X = numpy.empty((len(terms), 0))
M = numpy.array(terms, dtype=object)
termsTable = Orange.data.Table.from_numpy(termsDomain, X, metas=M)
else:
termsTable = Orange.data.Table(termsDomain)
self.send("Enrichment Report", termsTable)
def ViewSelectionChanged(self):
if self.selectionChanging:
return
self.selectionChanging = 1
self.selectedTerms = []
selected = self.listView.selectedItems()
self.selectedTerms = list(set([lvi.term.id for lvi in selected]))
self.ExampleSelection()
self.selectionChanging = 0
def TableSelectionChanged(self):
if self.selectionChanging:
return
self.selectionChanging = 1
self.selectedTerms = []
selectedIds = set([
self.sigTerms.itemFromIndex(index).goId
for index in self.sigTerms.selectedIndexes()
])
for i in range(self.sigTerms.topLevelItemCount()):
item = self.sigTerms.topLevelItem(i)
selected = item.goId in selectedIds
term = item.goId
if selected:
self.selectedTerms.append(term)
for lvi in self.termListViewItemDict[term]:
try:
lvi.setSelected(selected)
if selected:
lvi.setExpanded(True)
except RuntimeError: # Underlying C/C++ object deleted
pass
self.ExampleSelection()
self.selectionChanging = 0
def UpdateAddClassButton(self):
self.addClassCB.setEnabled(self.selectionDisjoint == 1)
def ExampleSelection(self):
self.commit()
def commit(self):
if self.clusterDataset is None:
return
terms = set(self.selectedTerms)
genes = reduce(operator.ior,
(set(self.graph[term][0]) for term in terms), set())
evidences = []
for etype in go.evidenceTypesOrdered:
if self.useEvidenceType[etype]:
# if getattr(self, "useEvidence" + etype):
evidences.append(etype)
allTerms = self.annotations.get_annotated_terms(
genes,
direct_annotation_only=self.selectionDirectAnnotation,
evidence_codes=evidences)
if self.selectionDisjoint > 0:
count = defaultdict(int)
for term in self.selectedTerms:
for g in allTerms.get(term, []):
count[g] += 1
ccount = 1 if self.selectionDisjoint == 1 else len(
self.selectedTerms)
selectedGenes = [
gene for gene, c in count.items()
if c == ccount and gene in genes
]
else:
selectedGenes = reduce(operator.ior,
(set(allTerms.get(term, []))
for term in self.selectedTerms), set())
if self.useAttrNames:
vars = [
self.clusterDataset.domain[gene] for gene in set(selectedGenes)
]
domain = Orange.data.Domain(vars,
self.clusterDataset.domain.class_vars,
self.clusterDataset.domain.metas)
newdata = self.clusterDataset.from_table(domain,
self.clusterDataset)
self.send("Data on Selected Genes", newdata)
self.send("Data on Unselected Genes", None)
elif self.candidateGeneAttrs:
selectedExamples = []
unselectedExamples = []
geneAttr = self.candidateGeneAttrs[min(
self.geneAttrIndex,
len(self.candidateGeneAttrs) - 1)]
if self.selectionDisjoint == 1:
goVar = Orange.data.DiscreteVariable("GO Term",
values=list(
self.selectedTerms))
newDomain = Orange.data.Domain(
self.clusterDataset.domain.variables, goVar,
self.clusterDataset.domain.metas)
goColumn = []
for i, ex in enumerate(self.clusterDataset):
if not numpy.isnan(ex[geneAttr]) and any(
gene in selectedGenes
for gene in str(ex[geneAttr]).split(",")):
if self.selectionDisjoint == 1 and self.selectionAddTermAsClass:
terms = filter(
lambda term: any(gene in self.graph[term][0]
for gene in str(ex[geneAttr]).
split(",")), self.selectedTerms)
term = sorted(terms)[0]
goColumn.append(goVar.values.index(term))
selectedExamples.append(i)
else:
unselectedExamples.append(i)
if selectedExamples:
selectedExamples = self.clusterDataset[selectedExamples]
if self.selectionDisjoint == 1 and self.selectionAddTermAsClass:
selectedExamples = Orange.data.Table.from_table(
newDomain, selectedExamples)
view, issparse = selectedExamples.get_column_view(goVar)
assert not issparse
view[:] = goColumn
else:
selectedExamples = None
if unselectedExamples:
unselectedExamples = self.clusterDataset[unselectedExamples]
else:
unselectedExamples = None
self.send("Data on Selected Genes", selectedExamples)
self.send("Data on Unselected Genes", unselectedExamples)
def ShowInfo(self):
dialog = QDialog(self)
dialog.setModal(False)
dialog.setLayout(QVBoxLayout())
label = QLabel(dialog)
label.setText(
"Ontology:\n" +
self.ontology.header if self.ontology else "Ontology not loaded!")
dialog.layout().addWidget(label)
label = QLabel(dialog)
label.setText("Annotations:\n" +
self.annotations.header.replace("!", "") if self.
annotations else "Annotations not loaded!")
dialog.layout().addWidget(label)
dialog.show()
def onDeleteWidget(self):
"""Called before the widget is removed from the canvas.
"""
self.annotations = None
self.ontology = None
gc.collect() # Force collection
class OWDatabasesUpdate(OWWidget):
name = "Databases Update"
description = "Update local systems biology databases."
icon = "../widgets/icons/OWDatabasesUpdate.svg"
priority = 1
inputs = []
outputs = []
want_main_area = False
def __init__(self, parent=None, signalManager=None,
name="Databases update"):
OWWidget.__init__(self, parent, signalManager, name,
wantMainArea=False)
self.searchString = ""
fbox = gui.widgetBox(self.controlArea, "Filter")
self.completer = TokenListCompleter(
self, caseSensitivity=Qt.CaseInsensitive)
self.lineEditFilter = QLineEdit(textChanged=self.SearchUpdate)
self.lineEditFilter.setCompleter(self.completer)
fbox.layout().addWidget(self.lineEditFilter)
box = gui.widgetBox(self.controlArea, "Files")
self.filesView = QTreeWidget(self)
self.filesView.setHeaderLabels(
["", "Data Source", "Update", "Last Updated", "Size"])
self.filesView.setRootIsDecorated(False)
self.filesView.setUniformRowHeights(True)
self.filesView.setSelectionMode(QAbstractItemView.NoSelection)
self.filesView.setSortingEnabled(True)
self.filesView.sortItems(1, Qt.AscendingOrder)
self.filesView.setItemDelegateForColumn(
0, UpdateOptionsItemDelegate(self.filesView))
self.filesView.model().layoutChanged.connect(self.SearchUpdate)
box.layout().addWidget(self.filesView)
box = gui.widgetBox(self.controlArea, orientation="horizontal")
self.updateButton = gui.button(
box, self, "Update all",
callback=self.UpdateAll,
tooltip="Update all updatable files",
)
self.downloadButton = gui.button(
box, self, "Download all",
callback=self.DownloadFiltered,
tooltip="Download all filtered files shown"
)
self.cancelButton = gui.button(
box, self, "Cancel", callback=self.Cancel,
tooltip="Cancel scheduled downloads/updates."
)
self.retryButton = gui.button(
box, self, "Reconnect", callback=self.RetrieveFilesList
)
self.retryButton.hide()
gui.rubber(box)
self.warning(0)
box = gui.widgetBox(self.controlArea, orientation="horizontal")
gui.rubber(box)
self.infoLabel = QLabel()
self.infoLabel.setAlignment(Qt.AlignCenter)
self.controlArea.layout().addWidget(self.infoLabel)
self.infoLabel.setSizePolicy(QSizePolicy.Expanding, QSizePolicy.Fixed)
self.updateItems = []
self.resize(800, 600)
self.progress = ProgressState(self, maximum=3)
self.progress.valueChanged.connect(self._updateProgress)
self.progress.rangeChanged.connect(self._updateProgress)
self.executor = ThreadExecutor(
threadPool=QThreadPool(maxThreadCount=2)
)
task = Task(self, function=self.RetrieveFilesList)
task.exceptionReady.connect(self.HandleError)
task.start()
self._tasks = []
self._haveProgress = False
def RetrieveFilesList(self):
self.retryButton.hide()
self.warning(0)
self.progress.setRange(0, 3)
task = Task(function=partial(retrieveFilesList, methodinvoke(self.progress, "advance")))
task.resultReady.connect(self.SetFilesList)
task.exceptionReady.connect(self.HandleError)
self.executor.submit(task)
self.setEnabled(False)
def SetFilesList(self, serverInfo):
"""
Set the files to show.
"""
self.setEnabled(True)
localInfo = serverfiles.allinfo()
all_tags = set()
self.filesView.clear()
self.updateItems = []
for item in join_info_dict(localInfo, serverInfo):
tree_item = UpdateTreeWidgetItem(item)
options_widget = UpdateOptionsWidget(item.state)
options_widget.item = item
options_widget.installClicked.connect(
partial(self.SubmitDownloadTask, item.domain, item.filename)
)
options_widget.removeClicked.connect(
partial(self.SubmitRemoveTask, item.domain, item.filename)
)
self.updateItems.append((item, tree_item, options_widget))
all_tags.update(item.tags)
self.filesView.addTopLevelItems(
[tree_item for _, tree_item, _ in self.updateItems]
)
for item, tree_item, options_widget in self.updateItems:
self.filesView.setItemWidget(tree_item, 0, options_widget)
# Add an update button if the file is updateable
if item.state == OUTDATED:
button = QToolButton(
None, text="Update",
maximumWidth=120,
minimumHeight=20,
maximumHeight=20
)
if sys.platform == "darwin":
button.setAttribute(Qt.WA_MacSmallSize)
button.clicked.connect(
partial(self.SubmitDownloadTask, item.domain,
item.filename)
)
self.filesView.setItemWidget(tree_item, 2, button)
self.progress.advance()
self.filesView.setColumnWidth(0, self.filesView.sizeHintForColumn(0))
for column in range(1, 4):
contents_hint = self.filesView.sizeHintForColumn(column)
header_hint = self.filesView.header().sectionSizeHint(column)
width = max(min(contents_hint, 400), header_hint)
self.filesView.setColumnWidth(column, width)
hints = [hint for hint in sorted(all_tags) if not hint.startswith("#")]
self.completer.setTokenList(hints)
self.SearchUpdate()
self.UpdateInfoLabel()
self.toggleButtons()
self.cancelButton.setEnabled(False)
self.progress.setRange(0, 0)
def buttonCheck(self, selected_items, state, button):
for item in selected_items:
if item.state != state:
button.setEnabled(False)
else:
button.setEnabled(True)
break
def toggleButtons(self):
selected_items = [item for item, tree_item, _ in self.updateItems if not tree_item.isHidden()]
self.buttonCheck(selected_items, OUTDATED, self.updateButton)
self.buttonCheck(selected_items, AVAILABLE, self.downloadButton)
def HandleError(self, exception):
if isinstance(exception, ConnectionError):
self.warning(0,
"Could not connect to server! Check your connection "
"and try to reconnect.")
self.SetFilesList({})
self.retryButton.show()
else:
sys.excepthook(type(exception), exception, None)
self.progress.setRange(0, 0)
self.setEnabled(True)
def UpdateInfoLabel(self):
local = [item for item, tree_item, _ in self.updateItems
if item.state != AVAILABLE and not tree_item.isHidden()]
size = sum(float(item.size) for item in local)
onServer = [item for item, tree_item, _ in self.updateItems if not tree_item.isHidden()]
sizeOnServer = sum(float(item.size) for item in onServer)
text = ("%i items, %s (on server: %i items, %s)" %
(len(local),
serverfiles.sizeformat(size),
len(onServer),
serverfiles.sizeformat(sizeOnServer)))
self.infoLabel.setText(text)
def UpdateAll(self):
self.warning(0)
for item, tree_item, _ in self.updateItems:
if item.state == OUTDATED and not tree_item.isHidden():
self.SubmitDownloadTask(item.domain, item.filename)
def DownloadFiltered(self):
# TODO: submit items in the order shown.
for item, tree_item, _ in self.updateItems:
if not tree_item.isHidden() and item.state in \
[AVAILABLE, OUTDATED]:
self.SubmitDownloadTask(item.domain, item.filename)
def SearchUpdate(self, searchString=None):
strings = str(self.lineEditFilter.text()).split()
for item, tree_item, _ in self.updateItems:
hide = not all(UpdateItem_match(item, string)
for string in strings)
tree_item.setHidden(hide)
self.UpdateInfoLabel()
self.toggleButtons()
def SubmitDownloadTask(self, domain, filename):
"""
Submit the (domain, filename) to be downloaded/updated.
"""
self.cancelButton.setEnabled(True)
index = self.updateItemIndex(domain, filename)
_, tree_item, opt_widget = self.updateItems[index]
task = DownloadTask(domain, filename, serverfiles.LOCALFILES)
self.progress.adjustRange(0, 100)
pb = ItemProgressBar(self.filesView)
pb.setRange(0, 100)
pb.setTextVisible(False)
task.advanced.connect(pb.advance)
task.advanced.connect(self.progress.advance)
task.finished.connect(pb.hide)
task.finished.connect(self.onDownloadFinished, Qt.QueuedConnection)
task.exception.connect(self.onDownloadError, Qt.QueuedConnection)
self.filesView.setItemWidget(tree_item, 2, pb)
# Clear the text so it does not show behind the progress bar.
tree_item.setData(2, Qt.DisplayRole, "")
pb.show()
# Disable the options widget
opt_widget.setEnabled(False)
self._tasks.append(task)
self.executor.submit(task)
def EndDownloadTask(self, task):
future = task.future()
index = self.updateItemIndex(task.domain, task.filename)
item, tree_item, opt_widget = self.updateItems[index]
self.filesView.removeItemWidget(tree_item, 2)
opt_widget.setEnabled(True)
if future.cancelled():
# Restore the previous state
tree_item.setUpdateItem(item)
opt_widget.setState(item.state)
elif future.exception():
tree_item.setUpdateItem(item)
opt_widget.setState(item.state)
# Show the exception string in the size column.
self.warning(0, "Error while downloading. Check your connection "
"and retry.")
# recreate button for download
button = QToolButton(
None, text="Retry",
maximumWidth=120,
minimumHeight=20,
maximumHeight=20
)
if sys.platform == "darwin":
button.setAttribute(Qt.WA_MacSmallSize)
button.clicked.connect(
partial(self.SubmitDownloadTask, item.domain,
item.filename)
)
self.filesView.setItemWidget(tree_item, 2, button)
else:
# get the new updated info dict and replace the the old item
self.warning(0)
info = serverfiles.info(item.domain, item.filename)
new_item = update_item_from_info(item.domain, item.filename,
info, info)
self.updateItems[index] = (new_item, tree_item, opt_widget)
tree_item.setUpdateItem(new_item)
opt_widget.setState(new_item.state)
self.UpdateInfoLabel()
def SubmitRemoveTask(self, domain, filename):
serverfiles.LOCALFILES.remove(domain, filename)
index = self.updateItemIndex(domain, filename)
item, tree_item, opt_widget = self.updateItems[index]
if item.info_server:
new_item = item._replace(state=AVAILABLE, local=None,
info_local=None)
else:
new_item = item._replace(local=None, info_local=None)
# Disable the options widget. No more actions can be performed
# for the item.
opt_widget.setEnabled(False)
tree_item.setUpdateItem(new_item)
opt_widget.setState(new_item.state)
self.updateItems[index] = (new_item, tree_item, opt_widget)
self.UpdateInfoLabel()
def Cancel(self):
"""
Cancel all pending update/download tasks (that have not yet started).
"""
for task in self._tasks:
task.future().cancel()
def onDeleteWidget(self):
self.Cancel()
self.executor.shutdown(wait=False)
OWWidget.onDeleteWidget(self)
def onDownloadFinished(self):
# on download completed/canceled/error
assert QThread.currentThread() is self.thread()
for task in list(self._tasks):
future = task.future()
if future.done():
self.EndDownloadTask(task)
self._tasks.remove(task)
if not self._tasks:
# Clear/reset the overall progress
self.progress.setRange(0, 0)
self.cancelButton.setEnabled(False)
def onDownloadError(self, exc_info):
sys.excepthook(*exc_info)
self.warning(0, "Error while downloading. Check your connection and "
"retry.")
def updateItemIndex(self, domain, filename):
for i, (item, _, _) in enumerate(self.updateItems):
if item.domain == domain and item.filename == filename:
return i
raise ValueError("%r, %r not in update list" % (domain, filename))
def _updateProgress(self, *args):
rmin, rmax = self.progress.range()
if rmin != rmax:
if not self._haveProgress:
self._haveProgress = True
self.progressBarInit()
self.progressBarSet(self.progress.ratioCompleted() * 100,
processEvents=None)
if rmin == rmax:
self._haveProgress = False
self.progressBarFinished()
class OWGenExpress(widget.OWWidget):
name = "GenExpress"
description = "Expression data from GenExpress."
icon = "../widgets/icons/GenCloud.svg"
priority = 36
inputs = []
outputs = [("Data", Orange.data.Table)]
username = settings.Setting("anonymous")
password = settings.Setting("")
log2 = settings.Setting(False)
transpose = settings.Setting(False)
rtypei = settings.Setting(0)
projecti = settings.Setting(0)
serveri = settings.Setting(0)
exnamei = settings.Setting(6)
excludeconstant = settings.Setting(False)
joinreplicates = settings.Setting(False)
currentSelection = settings.Setting(None)
experimentsHeaderState = settings.Setting(
{name: False
for _, name in HEADER[:ID_INDEX + 1]})
storedSortOrder = settings.Setting([])
storedSelections = settings.Setting([])
def __init__(self, parent=None):
super().__init__(parent)
self.servers = [
('https://dictyexpress.research.bcm.edu/', 'dictyExpress'),
('https://cloud.genialis.com/', 'Genialis'),
]
self.selectedExperiments = []
self.buffer = dicty.CacheSQLite(bufferfile)
self.searchString = ""
self.items = []
self.result_types = []
self.controlArea.setMaximumWidth(250)
self.controlArea.setMinimumWidth(250)
box = gui.widgetBox(self.controlArea, 'Project')
self.projectCB = gui.comboBox(box,
self,
"projecti",
items=[],
callback=self.ProjectChosen)
self.projects = []
b = gui.widgetBox(self.controlArea, "Selection bookmarks")
self.selectionSetsWidget = SelectionSetsWidget(self)
self.selectionSetsWidget.setSizePolicy(QSizePolicy.Preferred,
QSizePolicy.Maximum)
def store_selections(modified):
if not modified:
self.storedSelections = self.selectionSetsWidget.selections
self.selectionSetsWidget.selectionModified.connect(store_selections)
b.layout().addWidget(self.selectionSetsWidget)
gui.separator(self.controlArea)
b = gui.widgetBox(self.controlArea, "Sort output columns")
self.columnsSortingWidget = SortedListWidget(self)
self.columnsSortingWidget.setSizePolicy(QSizePolicy.Preferred,
QSizePolicy.Maximum)
box = gui.widgetBox(self.controlArea, 'Experiment name')
self.experimentNameCB = gui.comboBox(box,
self,
"exnamei",
items=SORTING_MODEL_LIST)
b.layout().addWidget(self.columnsSortingWidget)
sorting_model = QStringListModel(SORTING_MODEL_LIST)
self.columnsSortingWidget.setModel(sorting_model)
self.columnsSortingWidget.sortingOrder = self.storedSortOrder
def store_sort_order():
self.storedSortOrder = self.columnsSortingWidget.sortingOrder
self.columnsSortingWidget.sortingOrderChanged.connect(store_sort_order)
gui.separator(self.controlArea)
box = gui.widgetBox(self.controlArea, 'Expression Type')
self.expressionTypesCB = gui.comboBox(box,
self,
"rtypei",
items=[],
callback=self.UpdateResultsList)
gui.checkBox(self.controlArea, self, "excludeconstant",
"Exclude labels with constant values")
gui.checkBox(self.controlArea, self, "joinreplicates",
"Average replicates (use median)")
gui.checkBox(self.controlArea, self, "log2",
"Logarithmic (base 2) transformation")
gui.checkBox(self.controlArea, self, "transpose", "Genes as columns")
self.commit_button = gui.button(self.controlArea,
self,
"&Commit",
callback=self.Commit)
self.commit_button.setDisabled(True)
gui.rubber(self.controlArea)
box = gui.widgetBox(self.controlArea, 'Server')
gui.comboBox(box,
self,
"serveri",
items=[title for url, title in self.servers],
callback=self.ServerChosen)
gui.lineEdit(box,
self,
"username",
"Username:"******"password",
"Password:"******"Clear cache",
callback=self.clear_cache)
gui.lineEdit(self.mainArea,
self,
"searchString",
"Search",
callbackOnType=True,
callback=self.SearchUpdate)
self.headerLabels = [t[1] for t in HEADER]
self.experimentsWidget = QTreeWidget()
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.experimentsWidget.setSelectionMode(QTreeWidget.ExtendedSelection)
self.experimentsWidget.setRootIsDecorated(False)
self.experimentsWidget.setSortingEnabled(True)
contextEventFilter = gui.VisibleHeaderSectionContextEventFilter(
self.experimentsWidget, self.experimentsWidget)
self.experimentsWidget.header().installEventFilter(contextEventFilter)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.setAlternatingRowColors(True)
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.onSelectionChanged)
self.selectionSetsWidget.setSelectionModel(
self.experimentsWidget.selectionModel())
self.selectionSetsWidget.setSelections(self.storedSelections)
self.mainArea.layout().addWidget(self.experimentsWidget)
self.restoreHeaderState()
self.experimentsWidget.header().geometriesChanged.connect(
self.saveHeaderState)
self.dbc = None
self.AuthSet()
QTimer.singleShot(100, self.ConnectAndUpdate)
def sizeHint(self):
return QSize(800, 600)
def AuthSet(self):
if len(self.username):
self.passf.setDisabled(False)
else:
self.passf.setDisabled(True)
def AuthChanged(self):
self.AuthSet()
self.ConnectAndUpdate()
def ConnectAndUpdate(self):
self.Connect()
if self.dbc:
def get_data_count(project_id):
# XXX: is there a better way?
# Note: limit 0 would return all objects
return self.dbc.gen.api.data.get(
case_ids__contains=project_id,
type__startswith='data:expression:',
limit=1)['meta']['total_count']
self.projects = sorted([
p for p in self.dbc.projects().items()
if get_data_count(p[0]) > 0
],
key=lambda x: x[1])
self.UpdateProjects()
self.ProjectChosen()
self.UpdateExperimentTypes()
def Connect(self):
self.error(1)
self.warning(1)
username = '******'
password = '******'
url = self.servers[self.serveri][0]
if self.username:
username = self.username
password = self.password
if username.lower() in ['*****@*****.**', 'anonymous']:
username = '******'
password = '******'
self.dbc = None
self.projects = []
self.result_types = []
try:
self.dbc = Genesis(address=url,
username=username,
password=password,
cache=self.buffer)
except requests.exceptions.ConnectionError:
self.dbc = Genesis(address=url,
username=username,
password=password,
connect=False,
cache=self.buffer)
self.warning(1, "Could not connect to server, working from cache.")
except Exception:
self.error(1, "Wrong username or password.")
self.UpdateProjects()
self.UpdateExperimentTypes() # clear lists
def Reload(self):
self.UpdateExperiments(reload=True)
def clear_cache(self):
self.buffer.clear()
self.Reload()
def rtype(self):
"""Return selected result template type """
if self.result_types:
return self.result_types[self.rtypei]
else:
return None
def UpdateExperimentTypes(self):
self.expressionTypesCB.clear()
items = [self.result_types_labels[desc] for desc in self.result_types]
self.expressionTypesCB.addItems(items)
#do not update anything if the list is empty
if len(self.result_types):
self.rtypei = max(0, min(self.rtypei, len(self.result_types) - 1))
def UpdateProjects(self):
self.projectCB.clear()
items = [desc for pid, desc in self.projects]
self.projectCB.addItems(items)
#do not update anything if the list if empty
if len(self.projects) > 0:
self.projecti = max(0, min(self.projecti, len(self.projects) - 1))
def UpdateExperiments(self, reload=False):
self.experimentsWidget.clear()
if not self.dbc or not self.dbc.projectid: # the connection did not succeed
return
self.items = []
self.progressBarInit()
result_types = []
result_types_labels = []
sucind = False # success indicator for database index
try:
result_types, result_types_labels = self.dbc.result_types(
reload=reload)
sucind = True
except Exception:
try:
result_types, result_types_labels = self.dbc.result_types()
self.warning(0, "Can not access database - using cached data.")
sucind = True
except Exception:
self.error(0, "Can not access database.")
if sucind:
self.warning(0)
self.error(0)
self.result_types = result_types
self.result_types_labels = result_types_labels
self.UpdateExperimentTypes()
self.UpdateResultsList(reload=reload)
self.progressBarFinished()
if self.currentSelection:
self.currentSelection.select(
self.experimentsWidget.selectionModel())
self.handle_commit_button()
def ProjectChosen(self, reload=False):
if self.projects:
self.dbc.projectid = self.projects[self.projecti][0]
else:
self.dbc.projectid = None
self.UpdateExperiments(reload=reload)
def ServerChosen(self):
self.ConnectAndUpdate()
def UpdateResultsList(self, reload=False):
results_list = self.dbc.results_list(self.rtype(), reload=reload)
try:
results_list = self.dbc.results_list(self.rtype(), reload=reload)
except Exception:
try:
results_list = self.dbc.results_list(self.rtype())
except Exception:
self.error(0, "Can not access database.")
self.results_list = results_list
#softly change the view so that the selection stays the same
items_shown = {}
for i, item in enumerate(self.items):
c = str(item.text(ID_INDEX))
items_shown[c] = i
items_to_show = set(id_ for id_ in self.results_list)
add_items = set(items_to_show) - set(items_shown)
delete_items = set(items_shown) - set(items_to_show)
i = 0
while i < self.experimentsWidget.topLevelItemCount():
it = self.experimentsWidget.topLevelItem(i)
if str(it.text(ID_INDEX)) in delete_items:
self.experimentsWidget.takeTopLevelItem(i)
else:
i += 1
delete_ind = set([items_shown[i] for i in delete_items])
self.items = [
it for i, it in enumerate(self.items) if i not in delete_ind
]
for r_annot in add_items:
d = defaultdict(lambda: "?", self.results_list[r_annot])
row_items = [""] + [
to_text(d.get(key, "?")) for key, _ in HEADER[1:]
]
row_items[ID_INDEX] = r_annot
ci = MyTreeWidgetItem(self.experimentsWidget, row_items)
self.items.append(ci)
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
self.wantbufver = lambda x: self.results_list[x]["date_modified"]
self.UpdateCached()
def UpdateCached(self):
if self.wantbufver and self.dbc:
for item in self.items:
id = str(item.text(ID_INDEX))
version = self.dbc._in_buffer(id + "|||" + self.rtype())
value = " " if version == self.wantbufver(id) else ""
item.setData(0, Qt.DisplayRole, value)
def SearchUpdate(self, string=""):
for item in self.items:
item.setHidden(not all(s in item
for s in self.searchString.split()))
def Commit(self):
pb = gui.ProgressBar(self, iterations=100)
table = None
ids = []
for item in self.experimentsWidget.selectedItems():
unique_id = str(item.text(ID_INDEX))
ids.append(unique_id)
transfn = None
if self.log2:
transfn = lambda x: math.log(x + 1.0, 2)
reverse_header_dict = {name: name for key, name in HEADER}
reverse_header_dict["ID"] = "id"
allowed_labels = None
def namefn(a):
name = SORTING_MODEL_LIST[self.exnamei]
name = reverse_header_dict.get(name, "id")
return dict(a)[name]
if len(ids):
table = self.dbc.get_data(
ids=ids,
result_type=self.rtype(),
callback=pb.advance,
exclude_constant_labels=self.excludeconstant,
bufver=self.wantbufver,
transform=transfn,
allowed_labels=allowed_labels,
namefn=namefn)
if self.joinreplicates:
table = dicty.join_replicates(
table,
ignorenames=self.dbc.IGNORE_REPLICATE,
namefn="name",
avg=dicty.median,
fnshow=lambda x: " | ".join(map(str, x)))
# Sort attributes
sortOrder = self.columnsSortingWidget.sortingOrder
all_values = defaultdict(set)
for at in table.domain.attributes:
atts = at.attributes
for name in sortOrder:
all_values[name].add(
atts.get(reverse_header_dict[name], ""))
isnum = {}
for at, vals in all_values.items():
vals = filter(None, vals)
try:
for a in vals:
float(a)
isnum[at] = True
except ValueError:
isnum[at] = False
def optfloat(x, at):
if x == "":
return ""
else:
return float(x) if isnum[at] else x
def sorting_key(attr):
atts = attr.attributes
return tuple([
optfloat(atts.get(reverse_header_dict[name], ""), name)
for name in sortOrder
])
attributes = sorted(table.domain.attributes, key=sorting_key)
domain = Orange.data.Domain(attributes, table.domain.class_vars,
table.domain.metas)
table = Orange.data.Table.from_table(domain, table)
table = Orange.data.Table(domain, table)
if self.transpose:
experiments = [at for at in table.domain.variables]
attr = [
compat.ContinuousVariable.make(ex['DDB'].value)
for ex in table
]
metavars = sorted(table.domain.variables[0].attributes.keys())
metavars = [
compat.StringVariable.make(name) for name in metavars
]
domain = compat.create_domain(attr, None, metavars)
metavars = compat.get_metas(domain)
metas = [[exp.attributes[var.name] for var in metavars]
for exp in experiments]
table = compat.create_table(domain, table.X.transpose(), None,
metas)
data_hints.set_hint(table, "taxid", "352472")
data_hints.set_hint(table, "genesinrows", False)
self.send("Data", table)
self.UpdateCached()
pb.finish()
def onSelectionChanged(self, selected, deselected):
self.handle_commit_button()
def handle_commit_button(self):
self.currentSelection = \
SelectionByKey(self.experimentsWidget.selectionModel().selection(),
key=(ID_INDEX,))
self.commit_button.setDisabled(not len(self.currentSelection))
def saveHeaderState(self):
hview = self.experimentsWidget.header()
for i, label in enumerate(self.headerLabels):
self.experimentsHeaderState[label] = hview.isSectionHidden(i)
def restoreHeaderState(self):
hview = self.experimentsWidget.header()
state = self.experimentsHeaderState
for i, label in enumerate(self.headerLabels):
hview.setSectionHidden(i, state.get(label, True))
self.experimentsWidget.resizeColumnToContents(i)
class OWdictyExpress(OWWidget):
name = "dictyExpress"
description = "Time-course gene expression data"
icon = "../widgets/icons/OWdictyExpress.png"
want_main_area = True
priority = 3
class Inputs:
pass
class Outputs:
etc_data = Output("Data", Table)
class Error(OWWidget.Error):
unreachable_host = Msg('Host not reachable')
invalid_credentials = Msg('Invalid credentials')
username = settings.Setting('')
# password = settings.Setting('')
gene_as_attr_name = settings.Setting(0)
selected_item = settings.Setting(None, schema_only=True)
auto_commit = settings.Setting(False, schema_only=True)
def __init__(self):
super().__init__()
self.res = None
self.organism = '44689'
self.server = 'https://dictyexpress.research.bcm.edu'
self.headerLabels = [x[1] for x in Labels]
self.searchString = ""
self.items = []
self.progress_bar = None
# threads
self.threadpool = QThreadPool()
# Login Section
box = gui.widgetBox(self.controlArea, 'Login')
self.namefield = gui.lineEdit(box,
self,
"username",
"Username:"******"password",
"Password:"******"Output", addSpace=True)
gui.radioButtonsInBox(box,
self,
"gene_as_attr_name",
["Genes in rows", "Genes in columns"],
callback=self.invalidate)
self.controlArea.layout().addWidget(h_line())
self.refresh_button = gui.button(self.controlArea,
self,
"Refresh",
callback=self.refresh)
self.handle_cache_button(True)
gui.rubber(self.controlArea)
self.commit_button = gui.auto_commit(self.controlArea,
self,
"auto_commit",
"&Commit",
box=False)
# Experiment Section
label = QLabel("Available projects:")
my_font = QFont()
my_font.setBold(True)
label.setFont(my_font)
self.mainArea.layout().addWidget(label)
self.mainArea.layout().addWidget(h_line())
self.filter = gui.lineEdit(self.mainArea,
self,
"searchString",
"Filter:",
callbackOnType=True,
callback=self.search_update)
self.experimentsWidget = QTreeWidget(alternatingRowColors=True,
rootIsDecorated=False,
uniformRowHeights=True,
sortingEnabled=True)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.on_selection_changed)
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.mainArea.layout().addWidget(self.experimentsWidget)
self.auth_set()
self.connect()
self.sizeHint()
def sizeHint(self):
return QSize(1400, 680)
def auth_set(self):
self.passfield.setDisabled(not self.username)
def auth_changed(self):
self.auth_set()
self.connect()
def refresh(self):
self.reset()
self.load_experiments()
def reset(self):
self.experimentsWidget.clear() # clear QTreeWidget
self.items = []
# self.lastSelected = None
self.searchString = ""
def search_update(self):
parts = self.searchString.split()
for item in self.items:
item.setHidden(not all(s in item for s in parts))
def progress_advance(self):
# GUI should be updated in main thread. That's why we are calling advance method here
assert threading.current_thread() == threading.main_thread()
if self.progress_bar:
self.progress_bar.advance()
def handle_error(self, ex):
self.progress_bar.finish()
self.setStatusMessage('')
if isinstance(ex, ConnectionError) or isinstance(ex, ValueError):
self.Error.unreachable_host()
print(ex)
def load_experiments_result(self, experiments):
self.load_tree_items(experiments)
self.progress_bar.finish()
self.setStatusMessage('')
def connect(self):
self.res = None
self.Error.clear()
self.reset()
self.handle_cache_button(False)
user, password = resolwe.DEFAULT_EMAIL, resolwe.DEFAULT_PASSWD
if self.username or self.password:
user, password = self.username, self.password
try:
self.res = resolwe.connect(user, password, self.server, 'genesis')
except resolwe.ResolweAuthException:
self.Error.invalid_credentials()
else:
self.load_experiments()
self.handle_cache_button(True)
def load_experiments(self):
if self.res:
# init progress bar
self.progress_bar = gui.ProgressBar(self, iterations=2)
# status message
self.setStatusMessage('downloading experiments')
worker = Worker(self.res.fetch_etc_objects, progress_callback=True)
worker.signals.progress.connect(self.progress_advance)
worker.signals.result.connect(self.load_experiments_result)
worker.signals.error.connect(self.handle_error)
# move download process to worker thread
self.threadpool.start(worker)
def load_tree_items(self, list_of_exp):
self.items = [
CustomTreeItem(self.experimentsWidget, item)
for item in list_of_exp
]
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
self.set_cached_indicator()
self.set_selected()
def set_selected(self):
for item in self.items:
if self.selected_item and item.gen_data_id == self.selected_item:
self.experimentsWidget.setCurrentItem(item)
def on_selection_changed(self):
self.invalidate()
def invalidate(self):
self.commit()
def handle_cache_button(self, handle):
self.refresh_button.setEnabled(handle)
def send_to_output(self, result):
self.progress_bar.finish()
self.setStatusMessage('')
etc_json, table_name = result
# convert to table
data = etc_to_table(etc_json, bool(self.gene_as_attr_name))
# set table name
data.name = table_name
# match genes
gene_matcher = GeneMatcher(str(self.organism))
if not bool(self.gene_as_attr_name):
if 'Gene' in data.domain:
gene_column = data.domain['Gene']
gene_names = data.get_column_view(gene_column)[0]
gene_matcher.genes = gene_names
domain_ids = Domain([], metas=[StringVariable(ENTREZ_ID)])
data_ids = [[str(gene.gene_id) if gene.gene_id else '?']
for gene in gene_matcher.genes]
table_ids = Table(domain_ids, data_ids)
data = Table.concatenate([data, table_ids])
data.attributes[GENE_ID_COLUMN] = ENTREZ_ID
else:
gene_matcher.match_table_attributes(data)
data.attributes[GENE_ID_ATTRIBUTE] = ENTREZ_ID
# add table attributes
data.attributes[TAX_ID] = str(self.organism)
data.attributes[GENE_AS_ATTRIBUTE_NAME] = bool(self.gene_as_attr_name)
# reset cache indicators
self.set_cached_indicator()
# send data to the output signal
self.Outputs.etc_data.send(data)
def commit(self):
self.Error.clear()
selected_item = self.experimentsWidget.currentItem(
) # get selected TreeItem
self.selected_item = selected_item.gen_data_id
if selected_item:
# init progress bar
self.progress_bar = gui.ProgressBar(self, iterations=1)
# status message
self.setStatusMessage('downloading experiment data')
worker = Worker(
self.res.download_etc_data,
selected_item.gen_data_id,
table_name=selected_item.data_name,
progress_callback=True,
)
worker.signals.progress.connect(self.progress_advance)
worker.signals.result.connect(self.send_to_output)
worker.signals.error.connect(self.handle_error)
# move download process to worker thread
self.threadpool.start(worker)
def set_cached_indicator(self):
cached = self.res.get_cached_ids()
for item in self.items:
if item.gen_data_id in cached:
item.setData(0, Qt.DisplayRole, " ")
else:
item.setData(0, Qt.DisplayRole, "")
class OWGenExpress(widget.OWWidget):
name = "GenExpress"
description = "Expression data from GenExpress."
icon = "../widgets/icons/GenCloud.svg"
priority = 36
inputs = []
outputs = [("Data", Orange.data.Table)]
username = settings.Setting("anonymous")
password = settings.Setting("")
log2 = settings.Setting(False)
transpose = settings.Setting(False)
rtypei = settings.Setting(0)
projecti = settings.Setting(0)
serveri = settings.Setting(0)
exnamei = settings.Setting(6)
excludeconstant = settings.Setting(False)
joinreplicates = settings.Setting(False)
currentSelection = settings.Setting(None)
experimentsHeaderState = settings.Setting({
name: False for _, name in HEADER[:ID_INDEX + 1]}
)
storedSortOrder = settings.Setting([])
storedSelections = settings.Setting([])
def __init__(self, parent=None):
super().__init__(parent)
self.servers = [
('https://dictyexpress.research.bcm.edu/', 'dictyExpress'),
('https://cloud.genialis.com/', 'Genialis'),
]
self.selectedExperiments = []
self.buffer = dicty.CacheSQLite(bufferfile)
self.searchString = ""
self.items = []
self.result_types = []
self.controlArea.setMaximumWidth(250)
self.controlArea.setMinimumWidth(250)
box = gui.widgetBox(self.controlArea, 'Project')
self.projectCB = gui.comboBox(
box, self, "projecti", items=[], callback=self.ProjectChosen)
self.projects = []
b = gui.widgetBox(self.controlArea, "Selection bookmarks")
self.selectionSetsWidget = SelectionSetsWidget(self)
self.selectionSetsWidget.setSizePolicy(
QSizePolicy.Preferred, QSizePolicy.Maximum)
def store_selections(modified):
if not modified:
self.storedSelections = self.selectionSetsWidget.selections
self.selectionSetsWidget.selectionModified.connect(store_selections)
b.layout().addWidget(self.selectionSetsWidget)
gui.separator(self.controlArea)
b = gui.widgetBox(self.controlArea, "Sort output columns")
self.columnsSortingWidget = SortedListWidget(self)
self.columnsSortingWidget.setSizePolicy(
QSizePolicy.Preferred, QSizePolicy.Maximum)
box = gui.widgetBox(self.controlArea, 'Experiment name')
self.experimentNameCB = gui.comboBox(
box, self, "exnamei", items=SORTING_MODEL_LIST)
b.layout().addWidget(self.columnsSortingWidget)
sorting_model = QStringListModel(SORTING_MODEL_LIST)
self.columnsSortingWidget.setModel(sorting_model)
self.columnsSortingWidget.sortingOrder = self.storedSortOrder
def store_sort_order():
self.storedSortOrder = self.columnsSortingWidget.sortingOrder
self.columnsSortingWidget.sortingOrderChanged.connect(store_sort_order)
gui.separator(self.controlArea)
box = gui.widgetBox(self.controlArea, 'Expression Type')
self.expressionTypesCB = gui.comboBox(
box, self, "rtypei", items=[], callback=self.UpdateResultsList)
gui.checkBox(self.controlArea, self, "excludeconstant",
"Exclude labels with constant values")
gui.checkBox(self.controlArea, self, "joinreplicates",
"Average replicates (use median)")
gui.checkBox(self.controlArea, self, "log2",
"Logarithmic (base 2) transformation")
gui.checkBox(self.controlArea, self, "transpose",
"Genes as columns")
self.commit_button = gui.button(self.controlArea, self, "&Commit",
callback=self.Commit)
self.commit_button.setDisabled(True)
gui.rubber(self.controlArea)
box = gui.widgetBox(self.controlArea, 'Server')
gui.comboBox(box, self, "serveri",
items=[title for url, title in self.servers],
callback=self.ServerChosen)
gui.lineEdit(box, self, "username", "Username:"******"password", "Password:"******"Clear cache",
callback=self.clear_cache)
gui.lineEdit(self.mainArea, self, "searchString", "Search",
callbackOnType=True,
callback=self.SearchUpdate)
self.headerLabels = [t[1] for t in HEADER]
self.experimentsWidget = QTreeWidget()
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.experimentsWidget.setSelectionMode(QTreeWidget.ExtendedSelection)
self.experimentsWidget.setRootIsDecorated(False)
self.experimentsWidget.setSortingEnabled(True)
contextEventFilter = gui.VisibleHeaderSectionContextEventFilter(
self.experimentsWidget, self.experimentsWidget)
self.experimentsWidget.header().installEventFilter(contextEventFilter)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.setAlternatingRowColors(True)
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.onSelectionChanged)
self.selectionSetsWidget.setSelectionModel(
self.experimentsWidget.selectionModel())
self.selectionSetsWidget.setSelections(self.storedSelections)
self.mainArea.layout().addWidget(self.experimentsWidget)
self.restoreHeaderState()
self.experimentsWidget.header().geometriesChanged.connect(
self.saveHeaderState)
self.dbc = None
self.AuthSet()
QTimer.singleShot(100, self.ConnectAndUpdate)
def sizeHint(self):
return QSize(800, 600)
def AuthSet(self):
if len(self.username):
self.passf.setDisabled(False)
else:
self.passf.setDisabled(True)
def AuthChanged(self):
self.AuthSet()
self.ConnectAndUpdate()
def ConnectAndUpdate(self):
self.Connect()
if self.dbc:
def get_data_count(project_id):
# XXX: is there a better way?
# Note: limit 0 would return all objects
return self.dbc.gen.api.data.get(case_ids__contains=project_id,
type__startswith='data:expression:',
limit=1)['meta']['total_count']
self.projects = sorted([p for p in self.dbc.projects().items() if
get_data_count(p[0]) > 0], key=lambda x: x[1])
self.UpdateProjects()
self.ProjectChosen()
self.UpdateExperimentTypes()
def Connect(self):
self.error(1)
self.warning(1)
username = '******'
password = '******'
url = self.servers[self.serveri][0]
if self.username:
username = self.username
password = self.password
if username.lower() in ['*****@*****.**', 'anonymous']:
username = '******'
password = '******'
self.dbc = None
self.projects = []
self.result_types = []
try:
self.dbc = Genesis(
address=url, username=username, password=password,
cache=self.buffer)
except requests.exceptions.ConnectionError:
self.dbc = Genesis(
address=url, username=username, password=password,
connect=False, cache=self.buffer)
self.warning(1, "Could not connect to server, working from cache.")
except Exception:
self.error(1, "Wrong username or password.")
self.UpdateProjects()
self.UpdateExperimentTypes() # clear lists
def Reload(self):
self.UpdateExperiments(reload=True)
def clear_cache(self):
self.buffer.clear()
self.Reload()
def rtype(self):
"""Return selected result template type """
if self.result_types:
return self.result_types[self.rtypei]
else:
return None
def UpdateExperimentTypes(self):
self.expressionTypesCB.clear()
items = [self.result_types_labels[desc] for desc in self.result_types]
self.expressionTypesCB.addItems(items)
#do not update anything if the list is empty
if len(self.result_types):
self.rtypei = max(0, min(self.rtypei, len(self.result_types) - 1))
def UpdateProjects(self):
self.projectCB.clear()
items = [desc for pid, desc in self.projects]
self.projectCB.addItems(items)
#do not update anything if the list if empty
if len(self.projects) > 0:
self.projecti = max(0, min(self.projecti, len(self.projects) - 1))
def UpdateExperiments(self, reload=False):
self.experimentsWidget.clear()
if not self.dbc or not self.dbc.projectid: # the connection did not succeed
return
self.items = []
self.progressBarInit()
result_types = []
result_types_labels = []
sucind = False # success indicator for database index
try:
result_types, result_types_labels = self.dbc.result_types(reload=reload)
sucind = True
except Exception:
try:
result_types, result_types_labels = self.dbc.result_types()
self.warning(0, "Can not access database - using cached data.")
sucind = True
except Exception:
self.error(0, "Can not access database.")
if sucind:
self.warning(0)
self.error(0)
self.result_types = result_types
self.result_types_labels = result_types_labels
self.UpdateExperimentTypes()
self.UpdateResultsList(reload=reload)
self.progressBarFinished()
if self.currentSelection:
self.currentSelection.select(self.experimentsWidget.selectionModel())
self.handle_commit_button()
def ProjectChosen(self, reload=False):
if self.projects:
self.dbc.projectid = self.projects[self.projecti][0]
else:
self.dbc.projectid = None
self.UpdateExperiments(reload=reload)
def ServerChosen(self):
self.ConnectAndUpdate()
def UpdateResultsList(self, reload=False):
results_list = self.dbc.results_list(self.rtype(), reload=reload)
try:
results_list = self.dbc.results_list(self.rtype(), reload=reload)
except Exception:
try:
results_list = self.dbc.results_list(self.rtype())
except Exception:
self.error(0, "Can not access database.")
self.results_list = results_list
#softly change the view so that the selection stays the same
items_shown = {}
for i, item in enumerate(self.items):
c = str(item.text(ID_INDEX))
items_shown[c] = i
items_to_show = set(id_ for id_ in self.results_list)
add_items = set(items_to_show) - set(items_shown)
delete_items = set(items_shown) - set(items_to_show)
i = 0
while i < self.experimentsWidget.topLevelItemCount():
it = self.experimentsWidget.topLevelItem(i)
if str(it.text(ID_INDEX)) in delete_items:
self.experimentsWidget.takeTopLevelItem(i)
else:
i += 1
delete_ind = set([items_shown[i] for i in delete_items])
self.items = [it for i, it in enumerate(self.items)
if i not in delete_ind]
for r_annot in add_items:
d = defaultdict(lambda: "?", self.results_list[r_annot])
row_items = [""] + [to_text(d.get(key, "?"))
for key, _ in HEADER[1:]]
row_items[ID_INDEX] = r_annot
ci = MyTreeWidgetItem(self.experimentsWidget, row_items)
self.items.append(ci)
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
self.wantbufver = lambda x: self.results_list[x]["date_modified"]
self.UpdateCached()
def UpdateCached(self):
if self.wantbufver and self.dbc:
for item in self.items:
id = str(item.text(ID_INDEX))
version = self.dbc._in_buffer(id + "|||" + self.rtype())
value = " " if version == self.wantbufver(id) else ""
item.setData(0, Qt.DisplayRole, value)
def SearchUpdate(self, string=""):
for item in self.items:
item.setHidden(
not all(s in item for s in self.searchString.split()))
def Commit(self):
pb = gui.ProgressBar(self, iterations=100)
table = None
ids = []
for item in self.experimentsWidget.selectedItems():
unique_id = str(item.text(ID_INDEX))
ids.append(unique_id)
transfn = None
if self.log2:
transfn = lambda x: math.log(x + 1.0, 2)
reverse_header_dict = {name: name for key, name in HEADER}
reverse_header_dict["ID"] = "id"
allowed_labels = None
def namefn(a):
name = SORTING_MODEL_LIST[self.exnamei]
name = reverse_header_dict.get(name, "id")
return dict(a)[name]
if len(ids):
table = self.dbc.get_data(
ids=ids, result_type=self.rtype(),
callback=pb.advance,
exclude_constant_labels=self.excludeconstant,
bufver=self.wantbufver,
transform=transfn,
allowed_labels=allowed_labels,
namefn=namefn)
if self.joinreplicates:
table = dicty.join_replicates(table,
ignorenames=self.dbc.IGNORE_REPLICATE,
namefn="name",
avg=dicty.median,
fnshow=lambda x: " | ".join(map(str, x)))
# Sort attributes
sortOrder = self.columnsSortingWidget.sortingOrder
all_values = defaultdict(set)
for at in table.domain.attributes:
atts = at.attributes
for name in sortOrder:
all_values[name].add(atts.get(reverse_header_dict[name], ""))
isnum = {}
for at, vals in all_values.items():
vals = filter(None, vals)
try:
for a in vals:
float(a)
isnum[at] = True
except ValueError:
isnum[at] = False
def optfloat(x, at):
if x == "":
return ""
else:
return float(x) if isnum[at] else x
def sorting_key(attr):
atts = attr.attributes
return tuple([optfloat(atts.get(reverse_header_dict[name], ""), name)
for name in sortOrder])
attributes = sorted(table.domain.attributes, key=sorting_key)
domain = Orange.data.Domain(
attributes, table.domain.class_vars, table.domain.metas)
table = Orange.data.Table.from_table(domain, table)
table = Orange.data.Table(domain, table)
if self.transpose:
experiments = [at for at in table.domain.variables]
attr = [compat.ContinuousVariable.make(ex['DDB'].value) for ex in table]
metavars = sorted(table.domain.variables[0].attributes.keys())
metavars = [compat.StringVariable.make(name) for name in metavars]
domain = compat.create_domain(attr, None, metavars)
metas = [[exp.attributes[var.name] for var in metavars] for exp in experiments]
table = compat.create_table(domain, table.X.transpose(), None, metas)
data_hints.set_hint(table, "taxid", "352472")
data_hints.set_hint(table, "genesinrows", False)
self.send("Data", table)
self.UpdateCached()
pb.finish()
def onSelectionChanged(self, selected, deselected):
self.handle_commit_button()
def handle_commit_button(self):
self.currentSelection = \
SelectionByKey(self.experimentsWidget.selectionModel().selection(),
key=(ID_INDEX,))
self.commit_button.setDisabled(not len(self.currentSelection))
def saveHeaderState(self):
hview = self.experimentsWidget.header()
for i, label in enumerate(self.headerLabels):
self.experimentsHeaderState[label] = hview.isSectionHidden(i)
def restoreHeaderState(self):
hview = self.experimentsWidget.header()
state = self.experimentsHeaderState
for i, label in enumerate(self.headerLabels):
hview.setSectionHidden(i, state.get(label, True))
self.experimentsWidget.resizeColumnToContents(i)
class OWPIPAx(widget.OWWidget):
name = "PIPAx"
description = "Access data from PIPA RNA-Seq database."
icon = "../widgets/icons/PIPA.svg"
priority = 35
inputs = []
outputs = [("Data", Orange.data.Table)]
username = settings.Setting("")
password = settings.Setting("")
log2 = settings.Setting(False)
rtypei = settings.Setting(5) # hardcoded rpkm mapability polya
excludeconstant = settings.Setting(False)
joinreplicates = settings.Setting(False)
#: The stored current selection (in experiments view)
#: SelectionByKey | None
currentSelection = settings.Setting(None)
#: Stored selections (presets)
#: list of SelectionByKey
storedSelections = settings.Setting([])
#: Stored column sort keys (from Sort view)
#: list of strings
storedSortingOrder = settings.Setting(
["Strain", "Experiment", "Genotype", "Timepoint"])
experimentsHeaderState = settings.Setting(
{name: False
for _, name in HEADER[:ID_INDEX + 1]})
def __init__(self, parent=None, signalManager=None, name="PIPAx"):
super().__init__(parent)
self.selectedExperiments = []
self.buffer = dicty.CacheSQLite(bufferfile)
self.searchString = ""
self.result_types = []
self.mappings = {}
self.controlArea.setMaximumWidth(250)
self.controlArea.setMinimumWidth(250)
gui.button(self.controlArea, self, "Reload", callback=self.Reload)
gui.button(self.controlArea,
self,
"Clear cache",
callback=self.clear_cache)
b = gui.widgetBox(self.controlArea, "Experiment Sets")
self.selectionSetsWidget = SelectionSetsWidget(self)
self.selectionSetsWidget.setSizePolicy(QSizePolicy.Preferred,
QSizePolicy.Maximum)
def store_selections(modified):
if not modified:
self.storedSelections = self.selectionSetsWidget.selections
self.selectionSetsWidget.selectionModified.connect(store_selections)
b.layout().addWidget(self.selectionSetsWidget)
gui.separator(self.controlArea)
b = gui.widgetBox(self.controlArea, "Sort output columns")
self.columnsSortingWidget = SortedListWidget(self)
self.columnsSortingWidget.setSizePolicy(QSizePolicy.Preferred,
QSizePolicy.Maximum)
def store_sort_order():
self.storedSortingOrder = self.columnsSortingWidget.sortingOrder
self.columnsSortingWidget.sortingOrderChanged.connect(store_sort_order)
b.layout().addWidget(self.columnsSortingWidget)
sorting_model = QStringListModel(SORTING_MODEL_LIST)
self.columnsSortingWidget.setModel(sorting_model)
gui.separator(self.controlArea)
box = gui.widgetBox(self.controlArea, 'Expression Type')
self.expressionTypesCB = gui.comboBox(box,
self,
"rtypei",
items=[],
callback=self.UpdateResultsList)
gui.checkBox(self.controlArea, self, "excludeconstant",
"Exclude labels with constant values")
gui.checkBox(self.controlArea, self, "joinreplicates",
"Average replicates (use median)")
gui.checkBox(self.controlArea, self, "log2",
"Logarithmic (base 2) transformation")
self.commit_button = gui.button(self.controlArea,
self,
"&Commit",
callback=self.Commit)
self.commit_button.setDisabled(True)
gui.rubber(self.controlArea)
box = gui.widgetBox(self.controlArea, "Authentication")
gui.lineEdit(box,
self,
"username",
"Username:"******"password",
"Password:"******"searchString",
"Search",
callbackOnType=True,
callback=self.SearchUpdate)
self.headerLabels = [t[1] for t in HEADER]
self.experimentsWidget = QTreeWidget()
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.experimentsWidget.setSelectionMode(QTreeWidget.ExtendedSelection)
self.experimentsWidget.setRootIsDecorated(False)
self.experimentsWidget.setSortingEnabled(True)
contextEventFilter = gui.VisibleHeaderSectionContextEventFilter(
self.experimentsWidget, self.experimentsWidget)
self.experimentsWidget.header().installEventFilter(contextEventFilter)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.setAlternatingRowColors(True)
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.onSelectionChanged)
self.selectionSetsWidget.setSelectionModel(
self.experimentsWidget.selectionModel())
self.mainArea.layout().addWidget(self.experimentsWidget)
# Restore the selection states from the stored settings
self.selectionSetsWidget.selections = self.storedSelections
self.columnsSortingWidget.sortingOrder = self.storedSortingOrder
self.restoreHeaderState()
self.experimentsWidget.header().geometriesChanged.connect(
self.saveHeaderState)
self.dbc = None
self.AuthSet()
QTimer.singleShot(100, self.UpdateExperiments)
def sizeHint(self):
return QSize(800, 600)
def AuthSet(self):
if len(self.username):
self.passf.setDisabled(False)
else:
self.passf.setDisabled(True)
def AuthChanged(self):
self.AuthSet()
self.ConnectAndUpdate()
def ConnectAndUpdate(self):
self.Connect()
self.UpdateExperiments(reload=True)
def Connect(self):
self.error(1)
self.warning(1)
def en(x):
return x if len(x) else None
self.dbc = dicty.PIPAx(cache=self.buffer,
username=en(self.username),
password=self.password)
# check password
if en(self.username) != None:
try:
self.dbc.mappings(reload=True)
except dicty.AuthenticationError:
self.error(1, "Wrong username or password")
self.dbc = None
except Exception as ex:
print("Error when contacting the PIPA database", ex)
sys.excepthook(*sys.exc_info())
try: # maybe cached?
self.dbc.mappings()
self.warning(
1, "Can not access database - using cached data.")
except Exception as ex:
self.dbc = None
self.error(1, "Can not access database.")
def Reload(self):
self.UpdateExperiments(reload=True)
def clear_cache(self):
self.buffer.clear()
self.Reload()
def rtype(self):
"""Return selected result template type """
if self.result_types:
return self.result_types[self.rtypei][0]
else:
return "-1"
def UpdateExperimentTypes(self):
self.expressionTypesCB.clear()
items = [desc for _, desc in self.result_types]
self.expressionTypesCB.addItems(items)
self.rtypei = max(0, min(self.rtypei, len(self.result_types) - 1))
def UpdateExperiments(self, reload=False):
self.experimentsWidget.clear()
self.items = []
self.progressBarInit()
if not self.dbc:
self.Connect()
mappings = {}
result_types = []
sucind = False # success indicator for database index
try:
mappings = self.dbc.mappings(reload=reload)
result_types = self.dbc.result_types(reload=reload)
sucind = True
except Exception as ex:
try:
mappings = self.dbc.mappings()
result_types = self.dbc.result_types()
self.warning(0, "Can not access database - using cached data.")
sucind = True
except Exception as ex:
self.error(0, "Can not access database.")
if sucind:
self.warning(0)
self.error(0)
self.mappings = mappings
self.result_types = result_types
self.UpdateExperimentTypes()
self.UpdateResultsList(reload=reload)
self.progressBarFinished()
if self.currentSelection:
self.currentSelection.select(
self.experimentsWidget.selectionModel())
self.handle_commit_button()
def UpdateResultsList(self, reload=False):
results_list = {}
try:
results_list = self.dbc.results_list(self.rtype(), reload=reload)
except Exception as ex:
try:
results_list = self.dbc.results_list(self.rtype())
except Exception as ex:
self.error(0, "Can not access database.")
self.results_list = results_list
mappings_key_dict = dict(((m["data_id"], m["id"]), key) \
for key, m in self.mappings.items())
def mapping_unique_id(annot):
"""Map annotations dict from results_list to unique
`mappings` ids.
"""
data_id, mappings_id = annot["data_id"], annot["mappings_id"]
return mappings_key_dict[data_id, mappings_id]
elements = []
# softly change the view so that the selection stays the same
items_shown = {}
for i, item in enumerate(self.items):
c = str(item.text(10))
items_shown[c] = i
items_to_show = dict((mapping_unique_id(annot), annot)
for annot in self.results_list.values())
add_items = set(items_to_show) - set(items_shown)
delete_items = set(items_shown) - set(items_to_show)
i = 0
while i < self.experimentsWidget.topLevelItemCount():
it = self.experimentsWidget.topLevelItem(i)
if str(it.text(10)) in delete_items:
self.experimentsWidget.takeTopLevelItem(i)
else:
i += 1
delete_ind = set([items_shown[i] for i in delete_items])
self.items = [
it for i, it in enumerate(self.items) if i not in delete_ind
]
for r_annot in [items_to_show[i] for i in add_items]:
d = defaultdict(lambda: "?", r_annot)
row_items = [""] + [d.get(key, "?") for key, _ in HEADER[1:]]
try:
time_dict = literal_eval(row_items[DATE_INDEX])
date_rna = date(
time_dict["fullYearUTC"],
time_dict["monthUTC"] + 1, # Why is month 0 based?
time_dict["dateUTC"])
row_items[DATE_INDEX] = date_rna.strftime("%x")
except Exception:
row_items[DATE_INDEX] = ''
row_items[ID_INDEX] = mapping_unique_id(r_annot)
elements.append(row_items)
ci = MyTreeWidgetItem(self.experimentsWidget, row_items)
self.items.append(ci)
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
# which is the ok buffer version
# FIXME: what attribute to use for version?
self.wantbufver = \
lambda x, ad=self.results_list: \
defaultdict(lambda: "?", ad[x])["date"]
self.wantbufver = lambda x: "0"
self.UpdateCached()
def UpdateCached(self):
if self.wantbufver and self.dbc:
fn = self.dbc.download_key_function()
result_id_key = dict(((m["data_id"], m["mappings_id"]), key) \
for key, m in self.results_list.items())
for item in self.items:
c = str(item.text(10))
mapping = self.mappings[c]
data_id, mappings_id = mapping["data_id"], mapping["id"]
r_id = result_id_key[data_id, mappings_id]
# Get the buffered version
buffered = self.dbc.inBuffer(fn(r_id))
value = " " if buffered == self.wantbufver(r_id) else ""
item.setData(0, Qt.DisplayRole, value)
def SearchUpdate(self, string=""):
for item in self.items:
item.setHidden(not all(s in item \
for s in self.searchString.split())
)
def Commit(self):
if not self.dbc:
self.Connect()
pb = gui.ProgressBar(self, iterations=100)
table = None
ids = []
for item in self.experimentsWidget.selectedItems():
unique_id = str(item.text(10))
annots = self.mappings[unique_id]
ids.append((annots["data_id"], annots["id"]))
transfn = None
if self.log2:
transfn = lambda x: math.log(x + 1.0, 2)
reverse_header_dict = dict((name, key) for key, name in HEADER)
hview = self.experimentsWidget.header()
shownHeaders = [label for i, label in \
list(enumerate(self.headerLabels))[1:] \
if not hview.isSectionHidden(i)
]
allowed_labels = [reverse_header_dict.get(label, label) \
for label in shownHeaders]
if self.joinreplicates and "id" not in allowed_labels:
# need 'id' labels in join_replicates for attribute names
allowed_labels.append("id")
if len(ids):
table = self.dbc.get_data(
ids=ids,
result_type=self.rtype(),
callback=pb.advance,
exclude_constant_labels=self.excludeconstant,
# bufver=self.wantbufver,
transform=transfn,
allowed_labels=allowed_labels)
if self.joinreplicates:
table = dicty.join_replicates(table,
ignorenames=[
"replicate", "data_id",
"mappings_id", "data_name",
"id", "unique_id"
],
namefn=None,
avg=dicty.median)
# Sort attributes
sortOrder = self.columnsSortingWidget.sortingOrder
all_values = defaultdict(set)
for at in table.domain.attributes:
atts = at.attributes
for name in sortOrder:
all_values[name].add(
atts.get(reverse_header_dict[name], ""))
isnum = {}
for at, vals in all_values.items():
vals = filter(None, vals)
try:
for a in vals:
float(a)
isnum[at] = True
except:
isnum[at] = False
def optfloat(x, at):
if x == "":
return ""
else:
return float(x) if isnum[at] else x
def sorting_key(attr):
atts = attr.attributes
return tuple([optfloat(atts.get(reverse_header_dict[name], ""), name) \
for name in sortOrder])
attributes = sorted(table.domain.attributes, key=sorting_key)
domain = Orange.data.Domain(attributes, table.domain.class_var,
table.domain.metas)
table = table.from_table(domain, table)
data_hints.set_hint(table, "taxid", "352472")
data_hints.set_hint(table, "genesinrows", False)
self.send("Data", table)
self.UpdateCached()
pb.finish()
def onSelectionChanged(self, selected, deselected):
self.handle_commit_button()
def handle_commit_button(self):
self.currentSelection = \
SelectionByKey(self.experimentsWidget.selectionModel().selection(),
key=(1, 2, 3, 10))
self.commit_button.setDisabled(not len(self.currentSelection))
def saveHeaderState(self):
hview = self.experimentsWidget.header()
for i, label in enumerate(self.headerLabels):
self.experimentsHeaderState[label] = hview.isSectionHidden(i)
def restoreHeaderState(self):
hview = self.experimentsWidget.header()
state = self.experimentsHeaderState
for i, label in enumerate(self.headerLabels):
hview.setSectionHidden(i, state.get(label, True))
self.experimentsWidget.resizeColumnToContents(i)
class OWdictyExpress(OWWidget, ConcurrentWidgetMixin):
name = "dictyExpress"
description = "Time-course gene expression data"
icon = "../widgets/icons/OWdictyExpress.svg"
want_main_area = True
priority = 20
class Inputs:
pass
class Outputs:
etc_data = Output("Data", Table)
class Error(OWWidget.Error):
unreachable_host = Msg('Host not reachable')
invalid_credentials = Msg('Invalid credentials')
gene_as_attr_name = settings.Setting(0)
selected_item = settings.Setting(None, schema_only=True)
auto_commit = settings.Setting(False, schema_only=True)
def __init__(self):
super().__init__()
ConcurrentWidgetMixin.__init__(self)
self._res: Optional[genapi.GenAPI] = None
self.organism = '44689'
self.server = 'https://dictyexpress.research.bcm.edu'
self.headerLabels = [x[1] for x in Labels]
self.searchString = ""
self.items = []
self.genapi_pub_auth = {
'url': genapi.DEFAULT_URL,
'username': genapi.DEFAULT_EMAIL,
'password': genapi.DEFAULT_PASSWD,
}
# Login Section
box = gui.widgetBox(self.controlArea, 'Sign in')
self.user_info = gui.label(box, self, '')
self.server_info = gui.label(box, self, '')
box = gui.widgetBox(box, orientation=Qt.Horizontal)
self.sign_in_btn = gui.button(box,
self,
'Sign In',
callback=self.sign_in,
autoDefault=False)
self.sign_out_btn = gui.button(box,
self,
'Sign Out',
callback=self.sign_out,
autoDefault=False)
box = gui.widgetBox(self.controlArea, "Output")
gui.radioButtonsInBox(box,
self,
"gene_as_attr_name",
["Genes in rows", "Genes in columns"],
callback=self.invalidate)
self.clear_cache_btn = gui.button(self.controlArea,
self,
"Clear cache",
autoDefault=False,
callback=self.clear_cache)
gui.rubber(self.controlArea)
self.commit_button = gui.auto_commit(self.controlArea,
self,
"auto_commit",
"&Commit",
box=False)
# Experiment Section
label = QLabel("Available projects:")
my_font = QFont()
my_font.setBold(True)
label.setFont(my_font)
self.mainArea.layout().addWidget(label)
self.filter = gui.lineEdit(self.mainArea,
self,
"searchString",
"Filter:",
callbackOnType=True,
callback=self.search_update)
self.experimentsWidget = QTreeWidget(alternatingRowColors=True,
rootIsDecorated=False,
uniformRowHeights=True,
sortingEnabled=True)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.on_selection_changed)
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.mainArea.layout().addWidget(self.experimentsWidget)
self.sign_in(silent=True)
self.sizeHint()
def sizeHint(self):
return QSize(1400, 680)
@property
def res(self):
return self._res
@res.setter
def res(self, value: genapi.GenAPI):
if isinstance(value, genapi.GenAPI):
self._res = value
self.Error.clear()
self.reset()
self.load_experiments()
self.update_user_status()
self.Outputs.etc_data.send(None)
def sign_in(self, silent=False):
dialog = SignIn(self, server_type='genesis')
if silent:
dialog.sign_in()
if dialog.resolwe_instance is not None:
self.res = dialog.resolwe_instance
else:
self.res = connect(**self.genapi_pub_auth,
server_type=resolwe.GENESIS_PLATFORM)
if not silent and dialog.exec_():
self.res = dialog.resolwe_instance
def sign_out(self):
# Remove username and password
cm = get_credential_manager(resolwe.GENESIS_PLATFORM)
del cm.username
del cm.password
# Use public credentials when user signs out
self.res = connect(**self.genapi_pub_auth,
server_type=resolwe.GENESIS_PLATFORM)
def update_user_status(self):
cm = get_credential_manager(resolwe.GENESIS_PLATFORM)
if cm.username:
user_info = f"User: {cm.username}"
self.sign_in_btn.setEnabled(False)
self.sign_out_btn.setEnabled(True)
else:
user_info = 'User: Anonymous'
self.sign_in_btn.setEnabled(True)
self.sign_out_btn.setEnabled(False)
self.user_info.setText(user_info)
self.server_info.setText(f'Server: {self.res._gen.url[8:]}')
def clear_cache(self):
resolwe.GenAPI.clear_cache()
self.reset()
self.load_experiments()
def reset(self):
self.experimentsWidget.clear() # clear QTreeWidget
self.items = []
# self.lastSelected = None
self.searchString = ""
def search_update(self):
parts = self.searchString.split()
for item in self.items:
item.setHidden(not all(s in item for s in parts))
def on_exception(self, ex):
if isinstance(ex, ConnectionError) or isinstance(ex, ValueError):
self.Error.unreachable_host()
print(ex)
def on_done(self, results):
if isinstance(results, list):
self.load_tree_items(results)
elif isinstance(results, tuple):
self.send_to_output(results)
def load_experiments(self):
if self.res:
self.start(self.res.fetch_etc_objects)
def load_tree_items(self, list_of_exp):
self.items = [
CustomTreeItem(self.experimentsWidget, item)
for item in list_of_exp
]
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
self.set_cached_indicator()
self.set_selected()
def set_selected(self):
for item in self.items:
if self.selected_item and item.gen_data_id == self.selected_item:
self.experimentsWidget.setCurrentItem(item)
def on_selection_changed(self):
self.invalidate()
def invalidate(self):
self.commit()
def send_to_output(self, result):
etc_json, table_name = result
# convert to table
data = etc_to_table(etc_json, bool(self.gene_as_attr_name))
# set table name
data.name = table_name
# match genes
gene_matcher = GeneMatcher(str(self.organism))
if not bool(self.gene_as_attr_name):
if 'Gene' in data.domain:
data = gene_matcher.match_table_column(
data, 'Gene', StringVariable(ENTREZ_ID))
data.attributes[GENE_ID_COLUMN] = ENTREZ_ID
else:
data = gene_matcher.match_table_attributes(data)
data.attributes[GENE_ID_ATTRIBUTE] = ENTREZ_ID
# add table attributes
data.attributes[TAX_ID] = str(self.organism)
data.attributes[GENE_AS_ATTRIBUTE_NAME] = bool(self.gene_as_attr_name)
# reset cache indicators
self.set_cached_indicator()
# send data to the output signal
self.Outputs.etc_data.send(data)
def commit(self):
self.Error.clear()
selected_items = self.experimentsWidget.selectedItems(
) # get selected TreeItem
if len(selected_items) < 1:
self.Outputs.etc_data.send(None)
return
selected_item = selected_items[0]
self.selected_item = selected_item.gen_data_id
self.start(self.res.download_etc_data,
selected_item.gen_data_id,
table_name=selected_item.data_name)
def set_cached_indicator(self):
cached = self.res.get_cached_ids()
for item in self.items:
if item.gen_data_id in cached:
item.setData(0, Qt.DisplayRole, " ")
else:
item.setData(0, Qt.DisplayRole, "")
class OWdictyExpress(OWWidget):
name = "dictyExpress"
description = "Time-course gene expression data"
icon = "../widgets/icons/OWdictyExpress.png"
want_main_area = True
priority = 3
class Inputs:
pass
class Outputs:
etc_data = Output("Data", Table)
class Error(OWWidget.Error):
unreachable_host = Msg('Host not reachable')
invalid_credentials = Msg('Invalid credentials')
username = settings.Setting('')
password = settings.Setting('')
setTimeVariable = settings.Setting(False)
def __init__(self):
super().__init__()
self.res = None
self.orgnism = 352472
self.server = 'https://dictyexpress.research.bcm.edu'
self.headerLabels = [x[1] for x in Labels]
self.searchString = ""
self.items = []
self.lastSelected = None # store last selected customTreeItem
self.progress_bar = None
# threads
self.threadpool = QThreadPool()
# Login Section
box = gui.widgetBox(self.controlArea, 'Login')
self.namefield = gui.lineEdit(box,
self,
"username",
"Username:"******"password",
"Password:"******"setTimeVariable",
"Set Time variable")
self.time_var_checkBox.setToolTip(
'Create new column where each row represents one time point')
self.controlArea.layout().addWidget(h_line())
self.commit_button = gui.button(self.controlArea,
self,
"Commit",
callback=self.commit)
self.handle_commit_button(False)
self.refresh_button = gui.button(self.controlArea,
self,
"Refresh",
callback=self.refresh)
self.handle_cache_button(True)
gui.rubber(self.controlArea)
# Experiment Section
label = QLabel("Available projects:")
my_font = QFont()
my_font.setBold(True)
label.setFont(my_font)
self.mainArea.layout().addWidget(label)
self.mainArea.layout().addWidget(h_line())
self.filter = gui.lineEdit(self.mainArea,
self,
"searchString",
"Filter:",
callbackOnType=True,
callback=self.search_update)
self.experimentsWidget = QTreeWidget(alternatingRowColors=True,
rootIsDecorated=False,
uniformRowHeights=True,
sortingEnabled=True)
self.experimentsWidget.setItemDelegateForColumn(
0, gui.IndicatorItemDelegate(self, role=Qt.DisplayRole))
self.experimentsWidget.selectionModel().selectionChanged.connect(
self.onSelectionChanged)
self.experimentsWidget.setHeaderLabels(self.headerLabels)
self.mainArea.layout().addWidget(self.experimentsWidget)
self.auth_set()
self.connect()
def auth_set(self):
self.passfield.setDisabled(not self.username)
def auth_changed(self):
self.auth_set()
self.connect()
def refresh(self):
self.reset()
self.load_experiments()
def reset(self):
self.experimentsWidget.clear() # clear QTreeWidget
self.items = []
self.lastSelected = None
self.searchString = ""
self.handle_commit_button(False)
def search_update(self):
parts = self.searchString.split()
for item in self.items:
item.setHidden(not all(s in item for s in parts))
def progress_advance(self):
# GUI should be updated in main thread. That's why we are calling advance method here
assert threading.current_thread() == threading.main_thread()
if self.progress_bar:
self.progress_bar.advance()
def handle_error(self, ex):
self.progress_bar.finish()
self.setStatusMessage('')
if isinstance(ex, ConnectionError) or isinstance(ex, ValueError):
self.Error.unreachable_host()
print(ex)
def load_experiments_result(self, experiments):
self.load_tree_items(experiments)
self.progress_bar.finish()
self.setStatusMessage('')
def connect(self):
self.res = None
self.Error.clear()
self.reset()
self.handle_commit_button(False)
self.handle_cache_button(False)
user, password = resolwe.DEFAULT_EMAIL, resolwe.DEFAULT_PASSWD
if self.username or self.password:
user, password = self.username, self.password
try:
self.res = resolwe.connect(user, password, self.server, 'genesis')
except resolwe.ResolweAuthException as e:
self.Error.invalid_credentials()
else:
self.load_experiments()
self.handle_cache_button(True)
def load_experiments(self):
if self.res:
# init progress bar
self.progress_bar = gui.ProgressBar(self, iterations=3)
# status message
self.setStatusMessage('downloading experiments')
worker = Worker(self.res.fetch_etc_objects, progress_callback=True)
worker.signals.progress.connect(self.progress_advance)
worker.signals.result.connect(self.load_experiments_result)
worker.signals.error.connect(self.handle_error)
# move download process to worker thread
self.threadpool.start(worker)
def load_tree_items(self, list_of_exp):
self.items = [
CustomTreeItem(self.experimentsWidget, item)
for item in list_of_exp
]
for i in range(len(self.headerLabels)):
self.experimentsWidget.resizeColumnToContents(i)
def onSelectionChanged(self):
self.handle_commit_button(True)
def handle_commit_button(self, handle):
self.commit_button.setEnabled(handle)
def handle_cache_button(self, handle):
self.refresh_button.setEnabled(handle)
def send_to_output(self, etc_json):
self.progress_bar.finish()
self.setStatusMessage('')
data = etc_to_table(etc_json, self.setTimeVariable)
data.attributes[TAX_ID] = self.orgnism
data.attributes[GENE_AS_ATTRIBUTE_NAME] = self.setTimeVariable
self.Outputs.etc_data.send(data)
def commit(self):
self.Error.clear()
# init progress bar
self.progress_bar = gui.ProgressBar(self, iterations=3)
# status message
self.setStatusMessage('downloading experiment data')
selected_item = self.experimentsWidget.currentItem(
) # get selected TreeItem
if self.lastSelected:
self.lastSelected.setData(0, Qt.DisplayRole, "")
self.lastSelected = selected_item
selected_item.setData(0, Qt.DisplayRole, " ")
worker = Worker(self.res.download_etc_data,
selected_item.gen_data_id,
progress_callback=True)
worker.signals.progress.connect(self.progress_advance)
worker.signals.result.connect(self.send_to_output)
worker.signals.error.connect(self.handle_error)
# move download process to worker thread
self.threadpool.start(worker)
