dataBGR = bgr.removeBackground(data.astype('float'), size=(3, 3), verbose=True) #plt.plotTiling(dataBGR, inverse = True, z = (10,16)); from ClearMap.ImageProcessing.Filter.DoGFilter import filterDoG dataDoG = filterDoG(dataBGR, size=(8, 8, 4), verbose=True) #plt.plotTiling(dataDoG, inverse = True, z = (10,16)); from ClearMap.ImageProcessing.MaximaDetection import findExtendedMaxima dataMax = findExtendedMaxima(dataDoG, hMax=None, verbose=True, threshold=10) #plt.plotOverlayLabel( dataDoG / dataDoG.max(), dataMax.astype('int'), z = (10,16)) from ClearMap.ImageProcessing.MaximaDetection import findCenterOfMaxima cells = findCenterOfMaxima(data, dataMax) print(cells.shape) plt.plotOverlayPoints(data, cells, z=(10, 16)) from ClearMap.ImageProcessing.CellSizeDetection import detectCellShape dataShape = detectCellShape(dataDoG, cells, threshold=15) plt.plotOverlayLabel(dataDoG / dataDoG.max(), dataShape, z=(10, 16)) # find intensities / cell sizes from ClearMap.ImageProcessing.CellSizeDetection import findCellSize, findCellIntensity #size of cells cellSizes = findCellSize(dataShape, maxLabel=cells.shape[0]) #intensity of cells cellIntensities = findCellIntensity(dataBGR, dataShape, maxLabel=cells.shape[0])
import os import ClearMap.Settings as settings filename = os.path.join(settings.ClearMapPath, "Test/Data/ImageAnalysis/cfos-substack.tif") import ClearMap.Visualization.Plot as plt import ClearMap.IO as io data = io.readData(filename, z=(0, 26)) import ClearMap.ImageProcessing.BackgroundRemoval as bgr dataBGR = bgr.removeBackground(data.astype("float"), size=(3, 3), verbose=True) from ClearMap.ImageProcessing.Filter.DoGFilter import filterDoG dataDoG = filterDoG(dataBGR, size=(8, 8, 4), verbose=True) from ClearMap.ImageProcessing.MaximaDetection import findExtendedMaxima dataMax = findExtendedMaxima(dataDoG, hMax=None, verbose=True, threshold=10) from ClearMap.ImageProcessing.MaximaDetection import findCenterOfMaxima cells = findCenterOfMaxima(data, dataMax) plt.plotOverlayPoints(data, cells, z=(10, 16))
resultDirectory=None) CorrectionResamplingPointsInverseParameter["pointSource"] = points points = resamplePointsInverse(**CorrectionResamplingPointsInverseParameter) RegistrationResamplingPointParameter["pointSource"] = points points = resamplePoints(**RegistrationResamplingPointParameter) points = transformPoints( points, transformDirectory=RegistrationAlignmentParameter["resultDirectory"], indices=False, resultDirectory=None) io.writePoints(TransformedCellsFile, points) #Detect Cell Coordinates from ClearMap.ImageProcessing.MaximaDetection import findCenterOfMaxima cells = findCenterOfMaxima(data, dataMax) plt.plotOverlayPoints(data, cells, z=(1, 9)) print cells.shape #Detect Cell Coordinates from ClearMap.ImageProcessing.MaximaDetection import findCenterOfMaxima cells = findCenterOfMaxima(data, dataMax) print cells.shape #Cell Shape Detection from ClearMap.ImageProcessing.CellSizeDetection import detectCellShape dataShape = detectCellShape(dataDoG, cells, threshold=500) plt.plotOverlayLabel(dataDoG / dataDoG.max(), dataShape, z=(1, 9)) #Loading the results: points, intensities = io.readPoints(ImageProcessingParameter["sink"])