def _handle_convert(in_handle, in_format, out_handle, out_format, alphabet=None):
"""SeqIO conversion function (PRIVATE)."""
try:
f = _converter[(in_format, out_format)]
except KeyError:
f = None
if f:
return f(in_handle, out_handle, alphabet)
else:
records = SeqIO.parse(in_handle, in_format, alphabet)
return SeqIO.write(records, out_handle, out_format)
def __init__(self,
dir_path=None,
version=None,
scop=None,
astral_file=None,
db_handle=None):
"""
Initialise the astral database.
You must provide either a directory of SCOP files:
dir_path - string, the path to location of the scopseq-x.xx directory
(not the directory itself), and
version -a version number.
or, a FASTA file:
astral_file - string, a path to a fasta file (which will be loaded in memory)
or, a MYSQL database:
db_handle - a database handle for a MYSQL database containing a table
'astral' with the astral data in it. This can be created
using writeToSQL.
"""
if astral_file is None and dir_path is None and db_handle is None:
raise RuntimeError(
"Need either file handle, or (dir_path + " +
"version) or database handle to construct Astral")
if not scop:
raise RuntimeError("Must provide a Scop instance to construct")
self.scop = scop
self.db_handle = db_handle
if not astral_file and not db_handle:
if dir_path is None or version is None:
raise RuntimeError("must provide dir_path and version")
self.version = version
self.path = os.path.join(dir_path, "scopseq-%s" % version)
astral_file = "astral-scopdom-seqres-all-%s.fa" % self.version
astral_file = os.path.join(self.path, astral_file)
if astral_file:
#Build a dictionary of SeqRecord objects in the FASTA file, IN MEMORY
self.fasta_dict = SeqIO.to_dict(SeqIO.parse(astral_file, "fasta"))
self.astral_file = astral_file
self.EvDatasets = {}
self.EvDatahash = {}
self.IdDatasets = {}
self.IdDatahash = {}
def _SeqIO_to_alignment_iterator(handle,
format,
alphabet=None,
seq_count=None):
"""Uses Bio.SeqIO to create an MultipleSeqAlignment iterator (PRIVATE).
Arguments:
- handle - handle to the file.
- format - string describing the file format.
- alphabet - optional Alphabet object, useful when the sequence type
cannot be automatically inferred from the file itself
(e.g. fasta, phylip, clustal)
- seq_count - Optional integer, number of sequences expected in each
alignment. Recommended for fasta format files.
If count is omitted (default) then all the sequences in the file are
combined into a single MultipleSeqAlignment.
"""
from SAP.Bio import SeqIO
assert format in SeqIO._FormatToIterator
if seq_count:
#Use the count to split the records into batches.
seq_record_iterator = SeqIO.parse(handle, format, alphabet)
records = []
for record in seq_record_iterator:
records.append(record)
if len(records) == seq_count:
yield MultipleSeqAlignment(records, alphabet)
records = []
if len(records) > 0:
raise ValueError("Check seq_count argument, not enough sequences?")
else:
#Must assume that there is a single alignment using all
#the SeqRecord objects:
records = list(SeqIO.parse(handle, format, alphabet))
if records:
yield MultipleSeqAlignment(records, alphabet)
raise StopIteration
def align(cmdline,
pair,
kbyte=None,
force_type=None,
dry_run=False,
quiet=False,
debug=False):
"""
Returns a filehandle
"""
if not pair or len(pair) != 2:
raise ValueError("Expected pair of filename, not %s" % repr(pair))
output_file = tempfile.NamedTemporaryFile(mode='r')
input_files = tempfile.NamedTemporaryFile(
mode="w"), tempfile.NamedTemporaryFile(mode="w")
if dry_run:
print(
_build_align_cmdline(cmdline, pair, output_file.name, kbyte,
force_type, quiet))
return
for filename, input_file in zip(pair, input_files):
# Pipe the file through Biopython's Fasta parser/writer
# to make sure it conforms to the Fasta standard (in particular,
# Wise2 may choke on long lines in the Fasta file)
records = SeqIO.parse(open(filename), 'fasta')
SeqIO.write(records, input_file, 'fasta')
input_file.flush()
input_file_names = [input_file.name for input_file in input_files]
cmdline_str = _build_align_cmdline(cmdline, input_file_names,
output_file.name, kbyte, force_type,
quiet)
if debug:
sys.stderr.write("%s\n" % cmdline_str)
status = os.system(cmdline_str) >> 8
if status > 1:
if kbyte != 0: # possible memory problem; could be None
sys.stderr.write("INFO trying again with the linear model\n")
return align(cmdline, pair, 0, force_type, dry_run, quiet, debug)
else:
raise OSError("%s returned %s" % (" ".join(cmdline), status))
return output_file
def _SeqIO_to_alignment_iterator(handle, format, alphabet=None, seq_count=None):
"""Uses Bio.SeqIO to create an MultipleSeqAlignment iterator (PRIVATE).
Arguments:
- handle - handle to the file.
- format - string describing the file format.
- alphabet - optional Alphabet object, useful when the sequence type
cannot be automatically inferred from the file itself
(e.g. fasta, phylip, clustal)
- seq_count - Optional integer, number of sequences expected in each
alignment. Recommended for fasta format files.
If count is omitted (default) then all the sequences in the file are
combined into a single MultipleSeqAlignment.
"""
from SAP.Bio import SeqIO
assert format in SeqIO._FormatToIterator
if seq_count:
#Use the count to split the records into batches.
seq_record_iterator = SeqIO.parse(handle, format, alphabet)
records = []
for record in seq_record_iterator:
records.append(record)
if len(records) == seq_count:
yield MultipleSeqAlignment(records, alphabet)
records = []
if len(records) > 0:
raise ValueError("Check seq_count argument, not enough sequences?")
else:
#Must assume that there is a single alignment using all
#the SeqRecord objects:
records = list(SeqIO.parse(handle, format, alphabet))
if records:
yield MultipleSeqAlignment(records, alphabet)
raise StopIteration
def align(cmdline, pair, kbyte=None, force_type=None, dry_run=False, quiet=False, debug=False):
"""
Returns a filehandle
"""
if not pair or len(pair) != 2:
raise ValueError("Expected pair of filename, not %s" % repr(pair))
output_file = tempfile.NamedTemporaryFile(mode='r')
input_files = tempfile.NamedTemporaryFile(mode="w"), tempfile.NamedTemporaryFile(mode="w")
if dry_run:
print(_build_align_cmdline(cmdline,
pair,
output_file.name,
kbyte,
force_type,
quiet))
return
for filename, input_file in zip(pair, input_files):
# Pipe the file through Biopython's Fasta parser/writer
# to make sure it conforms to the Fasta standard (in particular,
# Wise2 may choke on long lines in the Fasta file)
records = SeqIO.parse(open(filename), 'fasta')
SeqIO.write(records, input_file, 'fasta')
input_file.flush()
input_file_names = [input_file.name for input_file in input_files]
cmdline_str = _build_align_cmdline(cmdline,
input_file_names,
output_file.name,
kbyte,
force_type,
quiet)
if debug:
sys.stderr.write("%s\n" % cmdline_str)
status = os.system(cmdline_str) >> 8
if status > 1:
if kbyte != 0: # possible memory problem; could be None
sys.stderr.write("INFO trying again with the linear model\n")
return align(cmdline, pair, 0, force_type, dry_run, quiet, debug)
else:
raise OSError("%s returned %s" % (" ".join(cmdline), status))
return output_file
def __init__( self, dir_path=None, version=None, scop=None,
astral_file=None, db_handle=None):
"""
Initialise the astral database.
You must provide either a directory of SCOP files:
dir_path - string, the path to location of the scopseq-x.xx directory
(not the directory itself), and
version -a version number.
or, a FASTA file:
astral_file - string, a path to a fasta file (which will be loaded in memory)
or, a MYSQL database:
db_handle - a database handle for a MYSQL database containing a table
'astral' with the astral data in it. This can be created
using writeToSQL.
"""
if astral_file is None and dir_path is None and db_handle is None:
raise RuntimeError("Need either file handle, or (dir_path + "
+ "version) or database handle to construct Astral")
if not scop:
raise RuntimeError("Must provide a Scop instance to construct")
self.scop = scop
self.db_handle = db_handle
if not astral_file and not db_handle:
if dir_path is None or version is None:
raise RuntimeError("must provide dir_path and version")
self.version = version
self.path = os.path.join( dir_path, "scopseq-%s" % version)
astral_file = "astral-scopdom-seqres-all-%s.fa" % self.version
astral_file = os.path.join(self.path, astral_file)
if astral_file:
#Build a dictionary of SeqRecord objects in the FASTA file, IN MEMORY
self.fasta_dict = SeqIO.to_dict(SeqIO.parse(astral_file, "fasta"))
self.astral_file = astral_file
self.EvDatasets = {}
self.EvDatahash = {}
self.IdDatasets = {}
self.IdDatahash = {}
def _handle_convert(in_handle,
in_format,
out_handle,
out_format,
alphabet=None):
"""SeqIO conversion function (PRIVATE)."""
try:
f = _converter[(in_format, out_format)]
except KeyError:
f = None
if f:
return f(in_handle, out_handle, alphabet)
else:
records = SeqIO.parse(in_handle, in_format, alphabet)
return SeqIO.write(records, out_handle, out_format)
def _count_codons(self, fasta_file):
with open(fasta_file, 'r') as handle:
# make the codon dictionary local
self.codon_count = CodonsDict.copy()
# iterate over sequence and count all the codons in the FastaFile.
for cur_record in SeqIO.parse(handle, "fasta"):
# make sure the sequence is lower case
if str(cur_record.seq).islower():
dna_sequence = str(cur_record.seq).upper()
else:
dna_sequence = str(cur_record.seq)
for i in range(0, len(dna_sequence), 3):
codon = dna_sequence[i:i+3]
if codon in self.codon_count:
self.codon_count[codon] += 1
else:
raise TypeError("illegal codon %s in gene: %s" % (codon, cur_record.id))
elif isinstance(value, (int, float, basestring)):
attr = {"name": key, "value": str(value)}
self.xml_generator.startElement("property",
AttributesImpl(attr))
self.xml_generator.endElement("property")
if __name__ == "__main__":
print("Running quick self test")
from SAP.Bio import SeqIO
import sys
with open("Tests/SeqXML/protein_example.xml", "r") as fileHandle:
records = list(SeqIO.parse(fileHandle, "seqxml"))
from SAP.Bio._py3k import StringIO
stringHandle = StringIO()
SeqIO.write(records, stringHandle, "seqxml")
SeqIO.write(records, sys.stdout, "seqxml")
print("")
stringHandle.seek(0)
records = list(SeqIO.parse(stringHandle, "seqxml"))
SeqIO.write(records, sys.stdout, "seqxml")
print("")
self.xml_generator.endElement("property")
elif isinstance(value, (int, float, basestring)):
attr = {"name": key, "value": str(value)}
self.xml_generator.startElement(
"property", AttributesImpl(attr))
self.xml_generator.endElement("property")
if __name__ == "__main__":
print("Running quick self test")
from SAP.Bio import SeqIO
import sys
with open("Tests/SeqXML/protein_example.xml", "r") as fileHandle:
records = list(SeqIO.parse(fileHandle, "seqxml"))
from SAP.Bio._py3k import StringIO
stringHandle = StringIO()
SeqIO.write(records, stringHandle, "seqxml")
SeqIO.write(records, sys.stdout, "seqxml")
print("")
stringHandle.seek(0)
records = list(SeqIO.parse(stringHandle, "seqxml"))
SeqIO.write(records, sys.stdout, "seqxml")
print("")