def init_gui(self):
layout = QGridLayout()
# self.setFixedSize(320,100)
self.box = roundQGroupBox()
self.box.setTitle("Stage")
box_layout = QGridLayout()
self.box.setLayout(box_layout)
self.setLayout(layout)
self.collect_data_button = QPushButton('Collect data')
self.collect_data_button.clicked.connect(self.start_aqcuisition)
box_layout.addWidget(self.collect_data_button)
layout.addWidget(self.box)
def init_gui(self):
# self.setFixedSize(325,100)
layout = QGridLayout()
self.setLayout(layout)
self.box = roundQGroupBox()
self.box.setTitle("Manual registration")
box_layout = QGridLayout()
self.box.setLayout(box_layout)
layout.addWidget(self.box)
self.open_button = QPushButton("Open")
self.open_button.clicked.connect(self.open_window)
box_layout.addWidget(self.open_button)
self.show()
def init_gui(self):
layout = QGridLayout()
# self.setFixedSize(240,200)
self.setFixedHeight(200)
self.box = roundQGroupBox()
self.box.setTitle("Galvo control")
box_layout = QGridLayout()
self.box.setLayout(box_layout)
self.setLayout(layout)
self.register_button = QPushButton('Register')
self.create_voltage_button = QPushButton('Create voltage')
self.points_per_contour_textbox = QLineEdit()
self.points_per_contour_textbox.setValidator(QtGui.QIntValidator())
self.points_per_contour_textbox.setText('100')
self.sampling_rate_textbox = QLineEdit()
self.sampling_rate_textbox.setValidator(QtGui.QIntValidator())
self.sampling_rate_textbox.setText('50000')
self.scan_button = QPushButton('Start scan')
self.scan_button.clicked.connect(self.scan)
self.register_button.clicked.connect(self.register)
self.create_voltage_button.clicked.connect(
self.request_mask_coordinates)
box_layout.addWidget(self.register_button, 1, 0)
box_layout.addWidget(QLabel('Points per contour:'))
box_layout.addWidget(self.points_per_contour_textbox, 2, 1)
box_layout.addWidget(QLabel('Sampling rate:'), 3, 0)
box_layout.addWidget(self.sampling_rate_textbox, 3, 1)
box_layout.addWidget(self.create_voltage_button, 4, 0)
box_layout.addWidget(self.scan_button, 4, 1)
layout.addWidget(self.box)
self.open_latest_transformation()
def __init__(self, *args, **kwargs):
super().__init__()
# self.setFixedSize(100,500)
layout = QGridLayout()
self.setLayout(layout)
self.image_viewer = SelectPointImageView()
layout.addWidget(self.image_viewer, 0, 0, 1, 3)
open_image_button = QPushButton("Open image from file")
retreive_image_from_camera_button = QPushButton("Load camera image")
open_image_button.clicked.connect(self.open_image_file)
retreive_image_from_camera_button.clicked.connect(
lambda: self.sig_request_camera_image.emit())
layout.addWidget(open_image_button, 1, 0, 1, 1)
layout.addWidget(retreive_image_from_camera_button, 1, 1, 1, 1)
self.coordinate_counter = QLabel("Saved coordinates: 0")
layout.addWidget(self.coordinate_counter, 2, 0)
self.filename_textbox = QLabel("File: ")
layout.addWidget(self.filename_textbox, 2, 1)
transformation_box = roundQGroupBox()
transformation_box.setTitle("Transformation")
transformation_box_layout = QGridLayout()
transformation_box.setLayout(transformation_box_layout)
self.transformation_order_spinbox = QSpinBox()
transformation_box_layout.addWidget(QLabel("Polynomial order:"), 0, 0)
transformation_box_layout.addWidget(self.transformation_order_spinbox,
0, 1)
find_transform_button = QPushButton("Find transform")
find_transform_button.clicked.connect(self.get_transformation)
transformation_box_layout.addWidget(find_transform_button, 2, 0, 1, 2)
layout.addWidget(transformation_box, 1, 2, 3, 1)
coords_container = roundQGroupBox()
coords_container.setTitle("Coordinates")
coords_container_layout = QGridLayout()
coords_container.setLayout(coords_container_layout)
abs_coordinate_label_x = QLabel("x: ")
self.abs_coordinate_textbox_x = QtWidgets.QLineEdit()
self.abs_coordinate_textbox_x.setValidator(QtGui.QDoubleValidator())
abs_coordinate_label_y = QLabel("y: ")
self.abs_coordinate_textbox_y = QtWidgets.QLineEdit()
self.abs_coordinate_textbox_y.setValidator(QtGui.QDoubleValidator())
img_coordinate_label_x = QLabel("x: ")
self.img_coordinate_textbox_x = QtWidgets.QLineEdit()
self.img_coordinate_textbox_x.setValidator(QtGui.QDoubleValidator())
img_coordinate_label_y = QLabel("y: ")
self.img_coordinate_textbox_y = QtWidgets.QLineEdit()
self.img_coordinate_textbox_y.setValidator(QtGui.QDoubleValidator())
get_crosshair_coords_button = QPushButton("Pick coordinate")
get_crosshair_coords_button.clicked.connect(
self.get_crosshair_coordinates)
save_coords_button = QPushButton("Save coordinates")
save_coords_button.clicked.connect(self.save_coords)
coords_container_layout.addWidget(QLabel("Absolute coordinates"), 0, 1,
1, 2)
coords_container_layout.addWidget(abs_coordinate_label_x, 1, 0)
coords_container_layout.addWidget(self.abs_coordinate_textbox_x, 1, 1)
coords_container_layout.addWidget(abs_coordinate_label_y, 2, 0)
coords_container_layout.addWidget(self.abs_coordinate_textbox_y, 2, 1)
coords_container_layout.addWidget(QLabel("Image coordinates"), 0, 4, 1,
1)
coords_container_layout.addWidget(img_coordinate_label_x, 1, 3)
coords_container_layout.addWidget(self.img_coordinate_textbox_x, 1, 4)
coords_container_layout.addWidget(img_coordinate_label_y, 2, 3)
coords_container_layout.addWidget(self.img_coordinate_textbox_y, 2, 4)
coords_container_layout.addWidget(get_crosshair_coords_button, 3, 4, 1,
1)
coords_container_layout.addWidget(save_coords_button, 4, 0, 1, 5)
layout.addWidget(coords_container, 3, 0, 3, 2)
self.found_transformation_container = roundQGroupBox()
self.found_transformation_container.setTitle("Result")
found_transformation_container_layout = QGridLayout()
self.found_transformation_container.setLayout(
found_transformation_container_layout)
self.found_transformation_text = QLabel("No transformation")
found_transformation_container_layout.addWidget(
self.found_transformation_text, 0, 0)
self.devices_dropdown = QComboBox()
devices = ["DMD: 640", "DMD: 532", "DMD: 488", "Galvos"]
self.devices_dropdown.addItems(devices)
self.cast_transformation_button = QPushButton("Cast")
self.cast_transformation_button.clicked.connect(
self.cast_transformation)
found_transformation_container_layout.addWidget(
QLabel("Cast to:"), 1, 0)
found_transformation_container_layout.addWidget(
self.devices_dropdown, 1, 1)
found_transformation_container_layout.addWidget(
self.cast_transformation_button, 2, 0, 1, 2)
layout.addWidget(self.found_transformation_container, 4, 2, 2, 1)
self.setMinimumSize(800, 1000)
self.show()
def init_gui(self):
self.setWindowTitle("Coordinate control")
self.layout = QGridLayout()
self.setMinimumSize(1250, 1000)
self.setLayout(self.layout)
self.image_mask_stack = QTabWidget()
self.selection_view = DrawingWidget(self)
self.selection_view.enable_drawing(True)
self.selection_view.getView().setLimits(xMin=0,
xMax=2048,
yMin=0,
yMax=2048,
minXRange=2048,
minYRange=2048,
maxXRange=2048,
maxYRange=2048)
self.selection_view.ui.roiBtn.hide()
self.selection_view.ui.menuBtn.hide()
self.selection_view.ui.normGroup.hide()
self.selection_view.ui.roiPlot.hide()
# self.selection_view.setImage(plt.imread('CoordinatesManager/Registration_Images/StageRegistration/Distance200_Offset0/A1.png'))
self.mask_view = SquareImageView()
self.mask_view.getView().setLimits(xMin=0,
xMax=2048,
yMin=0,
yMax=2048,
minXRange=2048,
minYRange=2048,
maxXRange=2048,
maxYRange=2048)
self.mask_view.ui.roiBtn.hide()
self.mask_view.ui.menuBtn.hide()
self.mask_view.ui.normGroup.hide()
self.mask_view.ui.roiPlot.hide()
self.mask_view.ui.histogram.hide()
self.image_mask_stack.addTab(self.selection_view, 'Select')
self.image_mask_stack.addTab(self.mask_view, 'Mask')
self.layout.addWidget(self.image_mask_stack, 0, 0, 5, 1)
# ---------------------- Mask generation Container --------------
self.maskGeneratorContainer = roundQGroupBox()
self.maskGeneratorContainer.setFixedSize(320, 220)
self.maskGeneratorContainer.setTitle("Mask generator")
self.maskGeneratorContainerLayout = QGridLayout()
self.maskGeneratorLayout = QGridLayout()
self.maskGeneratorContainer.setLayout(
self.maskGeneratorContainerLayout)
self.loadMaskFromFileButton = QPushButton('Open mask')
self.loadMaskFromFileButton.clicked.connect(self.load_mask_from_file)
self.addRoiButton = QPushButton("Add ROI")
self.createMaskButton = QPushButton("Create mask")
self.removeSelectionButton = QPushButton("Remove ROIs")
self.addRoiButton.clicked.connect(self.add_polygon_roi)
self.createMaskButton.clicked.connect(self.create_mask)
self.removeSelectionButton.clicked.connect(self.remove_selection)
self.maskGeneratorContainerLayout.addWidget(self.addRoiButton, 1, 0)
self.maskGeneratorContainerLayout.addWidget(self.createMaskButton, 2,
0)
self.maskGeneratorContainerLayout.addWidget(self.removeSelectionButton,
1, 1)
self.selectionOptionsContainer = roundQGroupBox()
self.selectionOptionsContainer.setTitle('Options')
self.selectionOptionsLayout = QGridLayout()
self.fillContourButton = QCheckBox()
self.invertMaskButton = QCheckBox()
self.thicknessSpinBox = QSpinBox()
self.thicknessSpinBox.setRange(1, 25)
self.selectionOptionsLayout.addWidget(QLabel('Fill contour:'), 0, 0)
self.selectionOptionsLayout.addWidget(self.fillContourButton, 0, 1)
self.selectionOptionsLayout.addWidget(QLabel('Invert mask:'), 1, 0)
self.selectionOptionsLayout.addWidget(self.invertMaskButton, 1, 1)
self.selectionOptionsLayout.addWidget(QLabel('Thickness:'), 2, 0)
self.selectionOptionsLayout.addWidget(self.thicknessSpinBox, 2, 1)
self.selectionOptionsContainer.setLayout(self.selectionOptionsLayout)
self.snapFovButton = QPushButton('Image FOV')
self.snapFovButton.clicked.connect(self.snap_fov)
self.maskGeneratorContainerLayout.addWidget(self.snapFovButton, 0, 0,
1, 1)
self.maskGeneratorContainerLayout.addWidget(
self.loadMaskFromFileButton, 0, 1, 1, 1)
self.maskGeneratorContainerLayout.addWidget(
self.selectionOptionsContainer, 2, 1, 2, 1)
self.layout.addWidget(self.maskGeneratorContainer, 0, 1)
self.DMDWidget = DMDWidget.DMDWidget()
self.layout.addWidget(self.DMDWidget, 1, 1)
self.DMDWidget.sig_request_mask_coordinates.connect(
lambda: self.cast_mask_coordinates('dmd'))
self.sig_cast_mask_coordinates_to_dmd.connect(
self.DMDWidget.receive_mask_coordinates)
self.DMDWidget.sig_start_registration.connect(
lambda: self.sig_start_registration.emit())
self.DMDWidget.sig_finished_registration.connect(
lambda: self.sig_finished_registration.emit())
self.GalvoWidget = GalvoWidget.GalvoWidget()
self.layout.addWidget(self.GalvoWidget, 2, 1)
self.GalvoWidget.sig_request_mask_coordinates.connect(
lambda: self.cast_mask_coordinates('galvo'))
self.sig_cast_mask_coordinates_to_galvo.connect(
self.GalvoWidget.receive_mask_coordinates)
self.GalvoWidget.sig_start_registration.connect(
lambda: self.sig_start_registration.emit())
self.GalvoWidget.sig_finished_registration.connect(
lambda: self.sig_finished_registration.emit())
self.ManualRegistrationWidget = ManualRegistration.ManualRegistrationWidget(
self)
self.ManualRegistrationWidget.sig_request_camera_image.connect(
self.cast_camera_image)
self.sig_cast_camera_image.connect(
self.ManualRegistrationWidget.receive_camera_image)
self.layout.addWidget(self.ManualRegistrationWidget, 3, 1)
self.StageRegistrationWidget = StageRegistrationWidget.StageWidget(
self)
self.layout.addWidget(self.StageRegistrationWidget, 4, 1)
def init_gui(self):
layout = QGridLayout()
# In microsec, the dark time between frame changes.
self.ALP_OFF_TIME = 44
self.setFixedSize(320, 450)
self.box = roundQGroupBox()
self.box.setTitle("DMD control")
box_layout = QGridLayout()
self.box.setLayout(box_layout)
self.setLayout(layout)
self.connect_button = QPushButton("Connect")
self.connect_button.setStyleSheet(
"QPushButton {background-color: #A3C1DA;}")
self.register_button = QPushButton("Register")
lasers = ["640", "532", "488"]
self.transform_for_laser_menu = QListWidget()
self.transform_for_laser_menu.addItems(lasers)
self.transform_for_laser_menu.setFixedHeight(52)
self.transform_for_laser_menu.setFixedWidth(82)
self.transform_for_laser_menu.setCurrentRow(0)
self.project_button = QPushButton("Start projecting")
self.project_button.setStyleSheet(
"QPushButton {background-color: #99FFCC;}")
self.clear_button = QPushButton("Clear memory")
self.white_project_button = QPushButton("Full illum.")
self.white_project_button.setStyleSheet(
"QPushButton {background-color: #FFE5CC;}")
self.load_mask_container_stack = QStackedWidget()
self.connect_button.clicked.connect(self.connect)
self.register_button.clicked.connect(lambda: self.register(
self.transform_for_laser_menu.selectedItems()[0].text()))
self.project_button.clicked.connect(self.project)
self.clear_button.clicked.connect(self.clear)
self.white_project_button.clicked.connect(self.project_full_white)
# Stack page 1
self.load_mask_container_1 = roundQGroupBox()
self.load_mask_container_1.setTitle("Load mask")
load_mask_container_layout_1 = QGridLayout()
self.load_mask_container_1.setLayout(load_mask_container_layout_1)
self.load_mask_from_widget_button = QPushButton("From mask generator")
self.load_mask_from_file_button = QPushButton("From file")
self.load_mask_from_widget_button.clicked.connect(
self.load_mask_from_widget)
self.load_mask_from_file_button.clicked.connect(
self.load_mask_from_memory)
load_mask_container_layout_1.addWidget(
self.load_mask_from_widget_button, 0, 0)
load_mask_container_layout_1.addWidget(self.load_mask_from_file_button,
1, 0)
# Stack page 2
self.load_mask_container_2 = roundQGroupBox()
self.load_mask_container_2.setTitle("Load mask")
load_mask_container_layout_2 = QGridLayout()
self.load_mask_container_2.setLayout(load_mask_container_layout_2)
self.load_image = QPushButton("Image")
self.load_folder = QPushButton("Folder")
self.load_video = QPushButton("Video")
self.back_load_mask_container_index0 = QPushButton("Back")
self.load_image.clicked.connect(self.load_mask_from_file)
self.load_folder.clicked.connect(self.load_mask_from_folder)
self.back_load_mask_container_index0.clicked.connect(
lambda: self.load_mask_container_stack.setCurrentIndex(0))
load_mask_container_layout_2.addWidget(self.load_image, 0, 0, 1, 2)
load_mask_container_layout_2.addWidget(self.load_folder, 1, 0, 1, 2)
load_mask_container_layout_2.addWidget(self.load_video, 2, 0)
load_mask_container_layout_2.addWidget(
self.back_load_mask_container_index0, 2, 1)
## Add layers to stack
self.load_mask_container_stack.addWidget(self.load_mask_container_1)
self.load_mask_container_stack.addWidget(self.load_mask_container_2)
# Detailed settings
self.settings_container = roundQGroupBox()
self.settings_container.setTitle("Settings")
settings_container_layout = QGridLayout()
self.settings_container.setLayout(settings_container_layout)
settings_container_layout.addWidget(QLabel("ALP_PROJ_MODE"), 0, 0)
self.ALP_PROJ_MODE_Combox = QComboBox()
self.ALP_PROJ_MODE_Combox.addItems(["ALP_MASTER", "ALP_SLAVE"])
self.ALP_PROJ_MODE_Combox.setToolTip(
"This ControlType is used to select from an internal or external trigger source."
)
settings_container_layout.addWidget(self.ALP_PROJ_MODE_Combox, 0, 1)
settings_container_layout.addWidget(QLabel("ALP_PROJ_STEP"), 2, 0)
self.ALP_PROJ_STEP_Combox = QComboBox()
self.ALP_PROJ_STEP_Combox.addItems(["ALP_DEFAULT", "ALP_EDGE_RISING"])
self.ALP_PROJ_STEP_Combox.setToolTip(
"Set frame switching trigger method in the sequence.(Only in ALP_MASTER mode)"
)
settings_container_layout.addWidget(self.ALP_PROJ_STEP_Combox, 2, 1)
settings_container_layout.addWidget(QLabel("ALP_BIN_MODE"), 3, 0)
self.ALP_ALP_BIN_MODE_Combox = QComboBox()
self.ALP_ALP_BIN_MODE_Combox.addItems(
["ALP_BIN_UNINTERRUPTED", "ALP_BIN_NORMAL"])
self.ALP_ALP_BIN_MODE_Combox.setToolTip(
"Binary mode: select from ALP_BIN_NORMAL and ALP_BIN_UNINTERRUPTED (No dark phase between frames)"
)
# self.ALP_ALP_BIN_MODE_Combox.currentIndexChanged.connect(self.set_repeat_from_BIN_MODE)
settings_container_layout.addWidget(self.ALP_ALP_BIN_MODE_Combox, 3, 1)
self.Illumination_time_textbox = QLineEdit()
self.Illumination_time_textbox.setValidator(QtGui.QIntValidator())
self.Illumination_time_textbox.setText("1000000") # Default 33334
self.repeat_imgseq_button = QCheckBox()
self.repeat_imgseq_button.setChecked(True)
self.repeat_imgseq_button.setToolTip("Repeat the sequence.")
Illumination_time_label = QLabel("Illumination time(µs):")
Illumination_time_label.setToolTip(
"Display time of a single image of the sequence in microseconds. PictureTime is set to minimize the dark time according to illuminationTime, +44 us e.g."
)
settings_container_layout.addWidget(Illumination_time_label, 4, 0)
settings_container_layout.addWidget(self.Illumination_time_textbox, 4,
1)
illumination_time_calculation_button = QPushButton(
"Fill in illu. time for Hz:")
settings_container_layout.addWidget(
illumination_time_calculation_button, 5, 0)
illumination_time_calculation_button.clicked.connect(
self.calculate_illumination_time_for_frequency)
self.expected_projection_frequency_textbox = QLineEdit()
self.expected_projection_frequency_textbox.setValidator(
QtGui.QIntValidator())
self.expected_projection_frequency_textbox.setText("2000")
settings_container_layout.addWidget(
self.expected_projection_frequency_textbox, 5, 1)
settings_container_layout.addWidget(QLabel("Repeat sequence:"), 6, 0)
settings_container_layout.addWidget(self.repeat_imgseq_button, 6, 1)
box_layout.addWidget(self.connect_button, 0, 0)
box_layout.addWidget(self.register_button, 0, 1)
box_layout.addWidget(QLabel("Register with laser:"), 1, 1)
box_layout.addWidget(self.transform_for_laser_menu, 2, 1, 2, 1)
box_layout.addWidget(self.project_button, 2, 0)
box_layout.addWidget(self.clear_button, 3, 0)
box_layout.addWidget(self.white_project_button, 1, 0)
box_layout.addWidget(self.load_mask_container_stack, 4, 0, 1, 2)
box_layout.addWidget(self.settings_container, 5, 0, 1, 2)
layout.addWidget(self.box)
self.open_latest_transformation()
def init_gui(self):
self.setWindowTitle("Coordinate control")
self.layout = QGridLayout()
self.setMinimumSize(1250, 1000)
self.setLayout(self.layout)
self.image_mask_stack = QTabWidget()
#---------------------------ROIs win----------------------------------
self.selection_view = DrawingWidget(self)
self.selection_view.setMinimumWidth(900)
self.selection_view.enable_drawing(True)
# self.selection_view.getView().setLimits(xMin = 0, xMax = 2048, yMin = 0, yMax = 2048, \
# minXRange = 2048, minYRange = 2048, maxXRange = 2048, maxYRange = 2048)
self.selection_view.ui.roiBtn.hide()
self.selection_view.ui.menuBtn.hide()
self.selection_view.ui.normGroup.hide()
self.selection_view.ui.roiPlot.hide()
# self.selection_view.setImage(plt.imread('CoordinatesManager/Registration_Images/StageRegistration/Distance200_Offset0/A1.png'))
#---------------------------Mask win----------------------------------
self.mask_view = SquareImageView()
self.mask_view.getView().setLimits(xMin = 0, xMax = 2048, yMin = 0, yMax = 2048, \
minXRange = 2048, minYRange = 2048, maxXRange = 2048, maxYRange = 2048)
self.mask_view.ui.roiBtn.hide()
self.mask_view.ui.menuBtn.hide()
self.mask_view.ui.normGroup.hide()
self.mask_view.ui.roiPlot.hide()
self.mask_view.ui.histogram.hide()
#-------------------------MAsk RCNN-----------------------------------
MLmaskviewBox = QWidget()
MLmaskviewBoxLayout = QGridLayout()
self.Matdisplay_Figure = Figure()
self.Matdisplay_Canvas = FigureCanvas(self.Matdisplay_Figure)
# self.Matdisplay_Canvas.setFixedWidth(900)
# self.Matdisplay_Canvas.setFixedHeight(900)
self.Matdisplay_Canvas.mpl_connect('button_press_event', self._onclick)
self.Matdisplay_toolbar = NavigationToolbar(self.Matdisplay_Canvas,
self)
MLmaskviewBoxLayout.addWidget(self.Matdisplay_toolbar, 0, 0)
MLmaskviewBoxLayout.addWidget(self.Matdisplay_Canvas, 1, 0)
MLmaskviewBox.setLayout(MLmaskviewBoxLayout)
self.image_mask_stack.addTab(self.selection_view, 'Select')
self.image_mask_stack.addTab(self.mask_view, 'Mask')
self.image_mask_stack.addTab(MLmaskviewBox, 'Mask-RCNN')
self.layout.addWidget(self.image_mask_stack, 0, 0, 4, 7)
# ---------------------- Mask generation Container --------------
self.maskGeneratorContainer = roundQGroupBox()
self.maskGeneratorContainer.setFixedSize(320, 220)
self.maskGeneratorContainer.setTitle("Mask generator")
self.maskGeneratorContainerLayout = QGridLayout()
self.maskGeneratorLayout = QGridLayout()
self.maskGeneratorContainer.setLayout(
self.maskGeneratorContainerLayout)
# self.loadMaskFromFileButton = QPushButton('Load mask')
# self.loadMaskFromFileButton.clicked.connect(self.load_mask_from_file)
self.addRoiButton = QPushButton("Add ROI")
self.createMaskButton = QPushButton("Add mask")
self.deleteMaskButton = QPushButton("Delete mask")
self.removeSelectionButton = cleanButton()
self.removeSelectionButton.setFixedHeight(25)
self.removeSelectionButton.setToolTip("Remove rois and output signals")
self.addRoiButton.clicked.connect(self.add_polygon_roi)
self.createMaskButton.clicked.connect(self.create_mask)
self.deleteMaskButton.clicked.connect(self.delete_mask)
self.removeSelectionButton.clicked.connect(self.remove_selection)
self.maskGeneratorContainerLayout.addWidget(self.addRoiButton, 0, 0)
self.maskGeneratorContainerLayout.addWidget(self.createMaskButton, 2,
0)
self.maskGeneratorContainerLayout.addWidget(self.deleteMaskButton, 2,
2)
self.maskGeneratorContainerLayout.addWidget(self.removeSelectionButton,
2, 3)
# self.maskGeneratorContainerLayout.addWidget(self.loadMaskFromFileButton, 2, 1)
self.clearRoiButton = QPushButton("Clear ROI")
self.clearRoiButton.clicked.connect(
lambda: self.selection_view.clear_rois())
self.maskGeneratorContainerLayout.addWidget(self.clearRoiButton, 0, 1)
self.maskGeneratorContainerLayout.addWidget(QLabel("Mask index:"), 1,
0)
self.mask_index_spinbox = QSpinBox()
self.mask_index_spinbox.setMinimum(1)
self.mask_index_spinbox.setValue(1)
self.maskGeneratorContainerLayout.addWidget(self.mask_index_spinbox, 1,
1)
self.previous_mask_button = QPushButton()
self.previous_mask_button.setStyleSheet(
"QPushButton {color:white;background-color: #FFCCE5;}"
"QPushButton:hover:!pressed {color:white;background-color: #CCFFFF;}"
)
self.previous_mask_button.setToolTip(
"Click arrow to enable WASD keyboard control")
self.previous_mask_button.setFixedWidth(60)
self.previous_mask_button.setIcon(QIcon('./Icons/LeftArrow.png'))
self.previous_mask_button.clicked.connect(
lambda: self.show_mask_with_index(-1))
self.maskGeneratorContainerLayout.addWidget(self.previous_mask_button,
1, 2)
self.next_mask_button = QPushButton()
self.next_mask_button.setStyleSheet(
"QPushButton {color:white;background-color: #FFCCE5;}"
"QPushButton:hover:!pressed {color:white;background-color: #CCFFFF;}"
)
self.next_mask_button.setToolTip(
"Click arrow to enable WASD keyboard control")
self.next_mask_button.setFixedWidth(60)
self.next_mask_button.setIcon(QIcon('./Icons/RightArrow.png'))
self.next_mask_button.clicked.connect(
lambda: self.show_mask_with_index(1))
self.maskGeneratorContainerLayout.addWidget(self.next_mask_button, 1,
3)
self.selectionOptionsContainer = roundQGroupBox()
self.selectionOptionsContainer.setTitle('Options')
self.selectionOptionsLayout = QGridLayout()
self.fillContourButton = QCheckBox()
self.invertMaskButton = QCheckBox()
self.thicknessSpinBox = QSpinBox()
self.thicknessSpinBox.setRange(1, 25)
self.selectionOptionsLayout.addWidget(QLabel('Fill contour:'), 0, 0)
self.selectionOptionsLayout.addWidget(self.fillContourButton, 0, 1)
self.selectionOptionsLayout.addWidget(QLabel('Invert mask:'), 1, 0)
self.selectionOptionsLayout.addWidget(self.invertMaskButton, 1, 1)
self.selectionOptionsLayout.addWidget(QLabel('Thickness:'), 2, 0)
self.selectionOptionsLayout.addWidget(self.thicknessSpinBox, 2, 1)
lasers = ['640', '532', '488']
self.transform_for_laser_menu = QListWidget()
self.transform_for_laser_menu.addItems(lasers)
self.transform_for_laser_menu.setFixedHeight(48)
self.transform_for_laser_menu.setFixedWidth(65)
self.transform_for_laser_menu.setCurrentRow(0)
self.selectionOptionsLayout.addWidget(QLabel('To be used with laser:'),
0, 2)
self.selectionOptionsLayout.addWidget(self.transform_for_laser_menu, 1,
2)
self.selectionOptionsContainer.setLayout(self.selectionOptionsLayout)
self.maskGeneratorContainerLayout.addWidget(
self.selectionOptionsContainer, 3, 0, 2, 3)
#----------------------------Mask-RCNN--------------------------------
self.MLOptionsContainer = roundQGroupBox()
self.MLOptionsContainer.setTitle('Mask-RCNN')
self.MLOptionsContainerLayout = QGridLayout()
self.init_ML_button = QPushButton('Init. ML', self)
self.MLOptionsContainerLayout.addWidget(self.init_ML_button, 0, 0)
self.init_ML_button.clicked.connect(
lambda: self.run_in_thread(self.init_ML))
self.run_ML_button = QPushButton('Analysis', self)
self.MLOptionsContainerLayout.addWidget(self.run_ML_button, 1, 0)
self.run_ML_button.clicked.connect(self.run_ML_onImg_and_display)
self.generate_MLmask_button = QPushButton('To ROIs', self)
self.MLOptionsContainerLayout.addWidget(self.generate_MLmask_button, 2,
0)
self.generate_MLmask_button.clicked.connect(
lambda: self.run_in_thread(self.generate_MLmask))
self.MLOptionsContainer.setLayout(self.MLOptionsContainerLayout)
self.maskGeneratorContainerLayout.addWidget(self.MLOptionsContainer, 3,
3, 2, 1)
self.layout.addWidget(self.maskGeneratorContainer, 0, 8, 1, 3)
self.DMDWidget = DMDWidget.DMDWidget()
self.layout.addWidget(self.DMDWidget, 1, 8, 1, 3)
"""--------------------------------------------------------------------
# Singal sent out from DMDWidget to ask for mask generated here.
# And then the generated roi list is sent back to function:receive_mask_coordinates in DMDWidget.
# --------------------------------------------------------------------
"""
self.DMDWidget.sig_request_mask_coordinates.connect(
lambda: self.cast_mask_coordinates('dmd'))
self.sig_cast_mask_coordinates_to_dmd.connect(
self.DMDWidget.receive_mask_coordinates)
self.DMDWidget.sig_start_registration.connect(
lambda: self.sig_start_registration.emit())
self.DMDWidget.sig_finished_registration.connect(
lambda: self.sig_finished_registration.emit())
#---------------------------Galvo control-----------------------------
self.GalvoWidget = GalvoWidget.GalvoWidget()
self.GalvoWidget.setFixedWidth(200)
self.GalvoWidget.setFixedHeight(180)
self.layout.addWidget(self.GalvoWidget, 2, 8, 2, 1)
self.GalvoWidget.sig_request_mask_coordinates.connect(
lambda: self.cast_mask_coordinates('galvo'))
self.sig_cast_mask_coordinates_to_galvo.connect(
self.GalvoWidget.receive_mask_coordinates)
self.GalvoWidget.sig_start_registration.connect(
lambda: self.sig_start_registration.emit())
self.GalvoWidget.sig_finished_registration.connect(
lambda: self.sig_finished_registration.emit())
#-------------------------Manual registration-------------------------
self.ManualRegistrationWidget = ManualRegistration.ManualRegistrationWidget(
)
self.ManualRegistrationWidget.setFixedWidth(100)
self.ManualRegistrationWidget.sig_request_camera_image.connect(
self.cast_camera_image)
self.sig_cast_camera_image.connect(
self.ManualRegistrationWidget.receive_camera_image)
self.layout.addWidget(self.ManualRegistrationWidget, 2, 9, 1, 1)
#-------------------------Stage collect-------------------------------
self.StageRegistrationWidget = StageRegistrationWidget.StageWidget()
self.StageRegistrationWidget.setFixedWidth(100)
self.layout.addWidget(self.StageRegistrationWidget, 3, 9, 1, 1)
def init_gui(self):
layout = QGridLayout()
self.setFixedSize(320, 400)
self.box = roundQGroupBox()
self.box.setTitle("DMD control")
box_layout = QGridLayout()
self.box.setLayout(box_layout)
self.setLayout(layout)
self.connect_button = QPushButton('Connect')
self.connect_button.setStyleSheet(
'QPushButton {background-color: #A3C1DA;}')
self.register_button = QPushButton('Register')
lasers = ['640', '532', '488']
self.transform_for_laser_menu = QListWidget()
self.transform_for_laser_menu.addItems(lasers)
self.transform_for_laser_menu.setFixedHeight(52)
self.transform_for_laser_menu.setFixedWidth(82)
self.transform_for_laser_menu.setCurrentRow(0)
self.project_button = QPushButton('Start projecting')
self.project_button.setStyleSheet(
'QPushButton {background-color: #99FFCC;}')
self.clear_button = QPushButton('Clear memory')
self.white_project_button = QPushButton('Full illum.')
self.white_project_button.setStyleSheet(
'QPushButton {background-color: #FFE5CC;}')
self.load_mask_container_stack = QStackedWidget()
self.connect_button.clicked.connect(self.connect)
self.register_button.clicked.connect(lambda: self.register(
self.transform_for_laser_menu.selectedItems()[0].text()))
self.project_button.clicked.connect(self.project)
self.clear_button.clicked.connect(self.clear)
self.white_project_button.clicked.connect(self.project_full_white)
# Stack page 1
self.load_mask_container_1 = roundQGroupBox()
self.load_mask_container_1.setTitle('Load mask')
load_mask_container_layout_1 = QGridLayout()
self.load_mask_container_1.setLayout(load_mask_container_layout_1)
self.load_mask_from_widget_button = QPushButton('From mask generator')
self.load_mask_from_file_button = QPushButton('From file')
self.load_mask_from_widget_button.clicked.connect(
self.load_mask_from_widget)
self.load_mask_from_file_button.clicked.connect(
self.load_mask_from_memory)
load_mask_container_layout_1.addWidget(
self.load_mask_from_widget_button, 0, 0)
load_mask_container_layout_1.addWidget(self.load_mask_from_file_button,
1, 0)
# Stack page 2
self.load_mask_container_2 = roundQGroupBox()
self.load_mask_container_2.setTitle('Load mask')
load_mask_container_layout_2 = QGridLayout()
self.load_mask_container_2.setLayout(load_mask_container_layout_2)
self.load_image = QPushButton('Image')
self.load_folder = QPushButton('Folder')
self.load_video = QPushButton('Video')
self.back_load_mask_container_index0 = QPushButton('Back')
self.load_image.clicked.connect(self.load_mask_from_file)
self.load_folder.clicked.connect(self.load_mask_from_folder)
self.back_load_mask_container_index0.clicked.connect(
lambda: self.load_mask_container_stack.setCurrentIndex(0))
load_mask_container_layout_2.addWidget(self.load_image, 0, 0, 1, 2)
load_mask_container_layout_2.addWidget(self.load_folder, 1, 0, 1, 2)
load_mask_container_layout_2.addWidget(self.load_video, 2, 0)
load_mask_container_layout_2.addWidget(
self.back_load_mask_container_index0, 2, 1)
## Add layers to stack
self.load_mask_container_stack.addWidget(self.load_mask_container_1)
self.load_mask_container_stack.addWidget(self.load_mask_container_2)
# Detailed settings
self.settings_container = roundQGroupBox()
self.settings_container.setTitle('Settings')
settings_container_layout = QGridLayout()
self.settings_container.setLayout(settings_container_layout)
settings_container_layout.addWidget(QLabel('ALP_PROJ_MODE'), 0, 0)
self.ALP_PROJ_MODE_Combox = QComboBox()
self.ALP_PROJ_MODE_Combox.addItems(['ALP_MASTER', 'ALP_SLAVE'])
self.ALP_PROJ_MODE_Combox.setToolTip(
"This ControlType is used to select from an internal or external trigger source."
)
settings_container_layout.addWidget(self.ALP_PROJ_MODE_Combox, 0, 1)
settings_container_layout.addWidget(QLabel('ALP_PROJ_STEP'), 2, 0)
self.ALP_PROJ_STEP_Combox = QComboBox()
self.ALP_PROJ_STEP_Combox.addItems(['ALP_DEFAULT', 'ALP_EDGE_RISING'])
self.ALP_PROJ_STEP_Combox.setToolTip(
"Set frame switching trigger method in the sequence.(Only in ALP_MASTER mode)"
)
settings_container_layout.addWidget(self.ALP_PROJ_STEP_Combox, 2, 1)
settings_container_layout.addWidget(QLabel('ALP_BIN_MODE'), 3, 0)
self.ALP_ALP_BIN_MODE_Combox = QComboBox()
self.ALP_ALP_BIN_MODE_Combox.addItems(
['ALP_BIN_UNINTERRUPTED', 'ALP_BIN_NORMAL'])
self.ALP_ALP_BIN_MODE_Combox.setToolTip(
"Binary mode: select from ALP_BIN_NORMAL and ALP_BIN_UNINTERRUPTED (No dark phase between frames)"
)
# self.ALP_ALP_BIN_MODE_Combox.currentIndexChanged.connect(self.set_repeat_from_BIN_MODE)
settings_container_layout.addWidget(self.ALP_ALP_BIN_MODE_Combox, 3, 1)
self.frame_rate_textbox = QLineEdit()
self.frame_rate_textbox.setValidator(QtGui.QIntValidator())
self.frame_rate_textbox.setText('1000000') # Default 33334
self.repeat_imgseq_button = QCheckBox()
self.repeat_imgseq_button.setChecked(True)
self.repeat_imgseq_button.setToolTip("Repeat the sequence.")
Illumination_time_label = QLabel(u"Illumination time(µs):")
Illumination_time_label.setToolTip(
"Display time of a single image of the sequence in microseconds. PictureTime is set to minimize the dark time according to illuminationTime, +44 us e.g."
)
settings_container_layout.addWidget(Illumination_time_label, 4, 0)
settings_container_layout.addWidget(self.frame_rate_textbox, 4, 1)
settings_container_layout.addWidget(QLabel('Repeat sequence:'), 5, 0)
settings_container_layout.addWidget(self.repeat_imgseq_button, 5, 1)
box_layout.addWidget(self.connect_button, 0, 0)
box_layout.addWidget(self.register_button, 0, 1)
box_layout.addWidget(QLabel('Register with laser:'), 1, 1)
box_layout.addWidget(self.transform_for_laser_menu, 2, 1, 2, 1)
box_layout.addWidget(self.project_button, 2, 0)
box_layout.addWidget(self.clear_button, 3, 0)
box_layout.addWidget(self.white_project_button, 1, 0)
box_layout.addWidget(self.load_mask_container_stack, 4, 0, 1, 2)
box_layout.addWidget(self.settings_container, 5, 0, 1, 2)
layout.addWidget(self.box)
self.open_latest_transformation()
def init_gui(self):
layout = QGridLayout()
self.setFixedSize(320, 300)
self.box = roundQGroupBox()
self.box.setTitle("DMD control")
box_layout = QGridLayout()
self.box.setLayout(box_layout)
self.setLayout(layout)
self.connect_button = QPushButton('Connect')
self.register_button = QPushButton('Register')
lasers = ['640', '532', '488']
self.transform_for_laser_menu = QListWidget()
self.transform_for_laser_menu.addItems(lasers)
self.transform_for_laser_menu.setFixedHeight(55)
self.transform_for_laser_menu.setCurrentRow(0)
self.project_button = QPushButton('Start projecting')
self.clear_button = QPushButton('Clear memory')
self.white_project_button = QPushButton('Full illum.')
self.load_mask_container_stack = QStackedWidget()
self.connect_button.clicked.connect(self.connect)
self.register_button.clicked.connect(lambda: self.register(
self.transform_for_laser_menu.selectedItems()[0].text()))
self.project_button.clicked.connect(self.project)
self.clear_button.clicked.connect(self.clear)
self.white_project_button.clicked.connect(self.project_full_white)
# Stack page 1
self.load_mask_container_1 = roundQGroupBox()
self.load_mask_container_1.setTitle('Load mask')
load_mask_container_layout_1 = QGridLayout()
self.load_mask_container_1.setLayout(load_mask_container_layout_1)
self.load_mask_from_widget_button = QPushButton('From mask generator')
self.load_mask_from_file_button = QPushButton('From file')
self.load_mask_from_widget_button.clicked.connect(
self.load_mask_from_widget)
self.load_mask_from_file_button.clicked.connect(
self.load_mask_from_memory)
load_mask_container_layout_1.addWidget(
self.load_mask_from_widget_button, 0, 0)
load_mask_container_layout_1.addWidget(self.load_mask_from_file_button,
1, 0)
# Stack page 2
self.load_mask_container_2 = roundQGroupBox()
self.load_mask_container_2.setTitle('Load mask')
load_mask_container_layout_2 = QGridLayout()
self.load_mask_container_2.setLayout(load_mask_container_layout_2)
self.load_image = QPushButton('Image')
self.load_folder = QPushButton('Folder')
self.load_video = QPushButton('Video')
self.load_image.clicked.connect(self.load_mask_from_file)
self.load_folder.clicked.connect(self.load_mask_from_folder)
load_mask_container_layout_2.addWidget(self.load_image, 0, 0)
load_mask_container_layout_2.addWidget(self.load_folder, 1, 0)
load_mask_container_layout_2.addWidget(self.load_video, 2, 0)
# Stack page 3
self.load_mask_container_3 = roundQGroupBox()
self.load_mask_container_3.setTitle('Load mask')
load_mask_container_layout_3 = QGridLayout()
self.load_mask_container_3.setLayout(load_mask_container_layout_3)
self.frame_rate_textbox = QLineEdit()
self.frame_rate_textbox.setValidator(QtGui.QIntValidator())
self.frame_rate_textbox.setText('1000000')
self.repeat_imgseq_button = QCheckBox()
self.confirm_button = QPushButton('Set')
self.confirm_button.clicked.connect(self.set_movie_settings_on_DMD)
load_mask_container_layout_3.addWidget(QLabel(u"Frame duration (µs):"),
0, 0)
load_mask_container_layout_3.addWidget(self.frame_rate_textbox, 0, 1)
load_mask_container_layout_3.addWidget(QLabel('Repeat sequence:'), 1,
0)
load_mask_container_layout_3.addWidget(self.repeat_imgseq_button, 1, 1)
load_mask_container_layout_3.addWidget(self.confirm_button, 2, 1)
## Add layers to stack
self.load_mask_container_stack.addWidget(self.load_mask_container_1)
self.load_mask_container_stack.addWidget(self.load_mask_container_2)
self.load_mask_container_stack.addWidget(self.load_mask_container_3)
box_layout.addWidget(self.connect_button, 0, 0)
box_layout.addWidget(self.register_button, 1, 0)
box_layout.addWidget(QLabel('To be used with laser:'), 0, 1)
box_layout.addWidget(self.transform_for_laser_menu, 1, 1, 2, 1)
box_layout.addWidget(self.project_button, 2, 0)
box_layout.addWidget(self.clear_button, 3, 0)
box_layout.addWidget(self.white_project_button, 3, 1)
box_layout.addWidget(self.load_mask_container_stack, 4, 0, 1, 2)
layout.addWidget(self.box)
self.open_latest_transformation()
