示例#1
0
def main():
    arglist = []
    for arg in sys.argv:
        arglist.append(arg)

    progname = os.path.basename(arglist[0])
    usage = progname + " prj_stack volume [begin end step] --CTF --npad=ntimes_padding --list=file --group=ID --snr=SNR --sym=symmetry --verbose=(0|1) --xysize --MPI"
    parser = OptionParser(usage, version=SPARXVERSION)

    parser.add_option("--CTF",
                      action="store_true",
                      default=False,
                      help="apply CTF correction")
    parser.add_option("--snr",
                      type="float",
                      default=1.0,
                      help="Signal-to-Noise Ratio")
    parser.add_option("--sym", type="string", default="c1", help="symmetry")
    parser.add_option(
        "--list",
        type="string",
        help="file with list of images to be used in the first column")
    parser.add_option(
        "--group",
        type="int",
        default=-1,
        help=
        "perform reconstruction using images for a given group number (group is attribute in the header)"
    )
    parser.add_option("--MPI",
                      action="store_true",
                      default=False,
                      help="use MPI version ")
    parser.add_option("--npad",
                      type="int",
                      default=2,
                      help="number of times padding (default 2)")
    parser.add_option("--verbose",
                      type="int",
                      default=0,
                      help="verbose level: 0 no verbose, 1 verbose")
    parser.add_option("--xysize",
                      type="int",
                      default=-1,
                      help="user expected size at xy direction")
    parser.add_option("--zsize",
                      type="int",
                      default=-1,
                      help="user expected size at z direction")
    parser.add_option("--smearstep",
                      type="float",
                      default=0.0,
                      help="Rotational smear step (default 0.0, no smear)")
    parser.add_option(
        "--interpolation_method",
        type="string",
        default="4nn",
        help="4nn, or tril: nearest neighbor, or trillinear interpolation")
    parser.add_option("--niter",
                      type="int",
                      default=10,
                      help="number of iterations for iterative reconstruction")
    parser.add_option("--upweighted",
                      action="store_true",
                      default=False,
                      help="apply background noise")
    parser.add_option("--compensate",
                      action="store_true",
                      default=False,
                      help="compensate in reconstruction")
    parser.add_option("--chunk_id",
                      type="int",
                      default=-1,
                      help="reconstruct both odd and even groups of particles")
    parser.add_option("--target_window_size",
                      type="int",
                      default=-1,
                      help=" size of the targeted reconstruction ")
    (options, args) = parser.parse_args(arglist[1:])

    if options.MPI:
        from mpi import mpi_init
        sys.argv = mpi_init(len(sys.argv), sys.argv)

    if global_def.CACHE_DISABLE:
        from utilities import disable_bdb_cache
        disable_bdb_cache()

    if len(args) == 2:
        prj_stack = args[0]
        vol_stack = args[1]
        nimage = EMUtil.get_image_count(prj_stack)
        pid_list = list(range(0, nimage))
    elif len(args) == 5:
        prj_stack = args[0]
        vol_stack = args[1]
        begin = atoi(args[2])
        end = atoi(args[3])
        step = atoi(args[4])
        pid_list = list(range(begin, end, step))
    else:
        ERROR("incomplete list of arguments", "recon3d_n", 1)
        exit()

    if (options.list and options.group > -1):
        ERROR("options group and list cannot be used together", "recon3d_n", 1)
        sys.exit()

    from applications import recons3d_n, recons3d_trl_MPI

    global_def.BATCH = True
    if options.interpolation_method == "4nn":
        recons3d_n(prj_stack, pid_list, vol_stack, options.CTF, options.snr, 1, options.npad,\
         options.sym, options.list, options.group, options.verbose, options.MPI,options.xysize, options.zsize, options.smearstep, options.upweighted, options.compensate,options.chunk_id)
    elif options.interpolation_method == "tril":
        if options.MPI is False:
            ERROR(
                " trillinear interpolation reconstruction has MPI version only!"
            )
            sys.exit()
        recons3d_trl_MPI(prj_stack, pid_list, vol_stack, options.CTF, options.snr, 1, options.npad,\
         options.sym, options.verbose, options.niter, options.compensate, options.target_window_size)

    else:
        ERROR(
            " Wrong interpolation method. The current options are 4nn, and tril. 4nn is the defalut one. "
        )

    global_def.BATCH = False

    if options.MPI:
        from mpi import mpi_finalize
        mpi_finalize()
示例#2
0
def main():
    arglist = []
    for arg in sys.argv:
        arglist.append(arg)

    progname = os.path.basename(arglist[0])
    usage = progname + " prj_stack volume [begin end step] --CTF --npad=ntimes_padding --list=file --group=ID --snr=SNR --sym=symmetry --verbose=(0|1) --xysize --MPI"
    parser = OptionParser(usage, version=SPARXVERSION)

    parser.add_option("--CTF",
                      action="store_true",
                      default=False,
                      help="apply CTF correction")
    parser.add_option("--snr",
                      type="float",
                      default=1.0,
                      help="Signal-to-Noise Ratio")
    parser.add_option("--sym", type="string", default="c1", help="symmetry")
    parser.add_option(
        "--list",
        type="string",
        help="file with list of images to be used in the first column")
    parser.add_option(
        "--group",
        type="int",
        default=-1,
        help=
        "perform reconstruction using images for a given group number (group is attribute in the header)"
    )
    parser.add_option("--MPI",
                      action="store_true",
                      default=False,
                      help="use MPI version ")
    parser.add_option("--npad",
                      type="int",
                      default=2,
                      help="number of times padding (default 2)")
    parser.add_option("--verbose",
                      type="int",
                      default=0,
                      help="verbose level: 0 no verbose, 1 verbose")
    parser.add_option("--xysize",
                      type="int",
                      default=-1,
                      help="user expected size at xy direction")
    parser.add_option("--zsize",
                      type="int",
                      default=-1,
                      help="user expected size at z direction")
    parser.add_option("--smearstep",
                      type="float",
                      default=0.0,
                      help="Rotational smear step (default 0.0, no smear)")

    (options, args) = parser.parse_args(arglist[1:])

    if options.MPI:
        from mpi import mpi_init
        sys.argv = mpi_init(len(sys.argv), sys.argv)

    if global_def.CACHE_DISABLE:
        from utilities import disable_bdb_cache
        disable_bdb_cache()

    if len(args) == 2:
        prj_stack = args[0]
        vol_stack = args[1]
        nimage = EMUtil.get_image_count(prj_stack)
        pid_list = range(0, nimage)
    elif len(args) == 5:
        prj_stack = args[0]
        vol_stack = args[1]
        begin = atoi(args[2])
        end = atoi(args[3])
        step = atoi(args[4])
        pid_list = range(begin, end, step)
    else:
        ERROR("incomplete list of arguments", "recon3d_n", 1)
        exit()

    if (options.list and options.group > -1):
        ERROR("options group and list cannot be used together", "recon3d_n", 1)
        sys.exit()

    from applications import recons3d_n

    global_def.BATCH = True
    recons3d_n(prj_stack, pid_list, vol_stack, options.CTF, options.snr, 1, options.npad,\
      options.sym, options.list, options.group, options.verbose, options.MPI,options.xysize, options.zsize, options.smearstep)
    global_def.BATCH = False

    if options.MPI:
        from mpi import mpi_finalize
        mpi_finalize()
示例#3
0
def main():
	arglist = []
	for arg in sys.argv:
		arglist.append( arg )

	progname = os.path.basename( arglist[0] )
	usage = progname + " prj_stack volume [begin end step] --CTF --npad=ntimes_padding --list=file --group=ID --snr=SNR --sym=symmetry --verbose=(0|1) --xysize --MPI"
	parser = OptionParser(usage, version=SPARXVERSION)

	parser.add_option("--CTF",     action="store_true", default=False, help="apply CTF correction")
	parser.add_option("--snr",     type="float",	    default=1.0,   help="Signal-to-Noise Ratio" )
	parser.add_option("--sym",     type="string",	    default="c1",  help="symmetry" )
	parser.add_option("--list",    type="string",                      help="file with list of images to be used in the first column" )
	parser.add_option("--group",   type="int",          default=-1,    help="perform reconstruction using images for a given group number (group is attribute in the header)" )
	parser.add_option("--MPI",     action="store_true", default=False, help="use MPI version ")
	parser.add_option("--npad",    type="int",	        default=2,     help="number of times padding (default 2)" )
	parser.add_option("--verbose", type="int",          default=0,     help="verbose level: 0 no verbose, 1 verbose" )
	parser.add_option("--xysize",  type="int",	        default=-1,    help="user expected size at xy direction" )
	parser.add_option("--zsize",   type="int",	        default=-1,    help="user expected size at z direction" )
	parser.add_option("--smearstep",   type="float",	default=0.0,   help="Rotational smear step (default 0.0, no smear)" )

	(options,args) = parser.parse_args(arglist[1:])


	if options.MPI:
		from mpi import mpi_init
		sys.argv = mpi_init(len(sys.argv), sys.argv)

	if global_def.CACHE_DISABLE:
		from utilities import disable_bdb_cache
		disable_bdb_cache()

	if len(args) == 2:
		prj_stack = args[0]
		vol_stack = args[1]
		nimage = EMUtil.get_image_count( prj_stack )
		pid_list = range(0, nimage)
	elif len(args) == 5:
		prj_stack = args[0]
		vol_stack = args[1]
		begin = atoi( args[2] )
		end   = atoi( args[3] )
		step  = atoi( args[4] )
		pid_list = range(begin, end, step)
	else:
		ERROR("incomplete list of arguments","recon3d_n",1)
		exit()

	if(options.list and options.group > -1):
		ERROR("options group and list cannot be used together","recon3d_n",1)
		sys.exit()

	from applications import recons3d_n

	global_def.BATCH = True
	recons3d_n(prj_stack, pid_list, vol_stack, options.CTF, options.snr, 1, options.npad,\
		 options.sym, options.list, options.group, options.verbose, options.MPI,options.xysize, options.zsize, options.smearstep)
	global_def.BATCH = False

	if options.MPI:
		from mpi import mpi_finalize
		mpi_finalize()
示例#4
0
def main():
	arglist = []
	for arg in sys.argv:
		arglist.append( arg )

	progname = os.path.basename( arglist[0] )
	usage = progname + " prj_stack volume [begin end step] --CTF --npad=ntimes_padding --list=file --group=ID --snr=SNR --sym=symmetry --verbose=(0|1) --xysize --MPI"
	parser = OptionParser(usage, version=SPARXVERSION)

	parser.add_option("--CTF",                    action="store_true",   default=False, help="apply CTF correction")
	parser.add_option("--snr",                    type="float",	         default=1.0,   help="Signal-to-Noise Ratio" )
	parser.add_option("--sym",                    type="string",	     default="c1",  help="symmetry" )
	parser.add_option("--list",                   type="string",                        help="file with list of images to be used in the first column" )
	parser.add_option("--group",                  type="int",           default=-1,     help="perform reconstruction using images for a given group number (group is attribute in the header)" )
	parser.add_option("--MPI",                    action="store_true",  default=False,  help="use MPI version ")
	parser.add_option("--npad",                   type="int",	        default=2,      help="number of times padding (default 2)" )
	parser.add_option("--verbose",                type="int",           default=0,      help="verbose level: 0 no verbose, 1 verbose" )
	parser.add_option("--xysize",                 type="int",	        default=-1,     help="user expected size at xy direction" )
	parser.add_option("--zsize",                  type="int",	        default=-1,     help="user expected size at z direction" )
	parser.add_option("--smearstep",              type="float",	        default=0.0,    help="Rotational smear step (default 0.0, no smear)" )
	parser.add_option("--interpolation_method",   type ="string",	    default="4nn",  help="4nn, or tril: nearest neighbor, or trillinear interpolation" )
	parser.add_option("--niter",                  type="int",	        default=10,     help="number of iterations for iterative reconstruction" )
	parser.add_option("--upweighted",             action="store_true",  default=False,  help="apply background noise")
	parser.add_option("--compensate",             action="store_true",  default=False,  help="compensate in reconstruction")
	parser.add_option("--chunk_id",               type="int",           default=-1,     help="reconstruct both odd and even groups of particles")
	parser.add_option("--target_window_size",               type="int",           default=-1,     help=" size of the targeted reconstruction ")
	(options,args) = parser.parse_args(arglist[1:])


	if options.MPI:
		from mpi import mpi_init
		sys.argv = mpi_init(len(sys.argv), sys.argv)

	if global_def.CACHE_DISABLE:
		from utilities import disable_bdb_cache
		disable_bdb_cache()

	if len(args) == 2:
		prj_stack = args[0]
		vol_stack = args[1]
		nimage = EMUtil.get_image_count( prj_stack )
		pid_list = range(0, nimage)
	elif len(args) == 5:
		prj_stack = args[0]
		vol_stack = args[1]
		begin = atoi( args[2] )
		end   = atoi( args[3] )
		step  = atoi( args[4] )
		pid_list = range(begin, end, step)
	else:
		ERROR("incomplete list of arguments","recon3d_n",1)
		exit()

	if(options.list and options.group > -1):
		ERROR("options group and list cannot be used together","recon3d_n",1)
		sys.exit()

	from applications import recons3d_n, recons3d_trl_MPI

	global_def.BATCH = True
	if options.interpolation_method == "4nn":
		recons3d_n(prj_stack, pid_list, vol_stack, options.CTF, options.snr, 1, options.npad,\
		 options.sym, options.list, options.group, options.verbose, options.MPI,options.xysize, options.zsize, options.smearstep, options.upweighted, options.compensate,options.chunk_id)
	elif options.interpolation_method == "tril":
		if options.MPI is False:
			ERROR(" trillinear interpolation reconstruction has MPI version only!")
			sys.exit()
		recons3d_trl_MPI(prj_stack, pid_list, vol_stack, options.CTF, options.snr, 1, options.npad,\
		 options.sym, options.verbose, options.niter, options.compensate, options.target_window_size)
		
	else:
		ERROR(" Wrong interpolation method. The current options are 4nn, and tril. 4nn is the defalut one. ")
		
	global_def.BATCH = False

	if options.MPI:
		from mpi import mpi_finalize
		mpi_finalize()