def getArgsFastq(fastq_links, input_parameters, debug):
# initialize parameters
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: getArgsFastq():'])
if debug: debug_list.append(['-'])
if debug: debug_list.append(['ref_name', 'fastq_path', 'fastq_num'])
if debug: debug_list.append(['-'])
SEQ = True if input_parameters['seq_style_fq'].lower() in ['seq', 'true'
] else False
# open fastq links file and save links to {<ref_name>:<fastq_path>} dictionary
fastq_files = {}
fastq_ref = {}
first = True if SEQ else False
ref_name, fastq_path, num_reads = ('', ) * 3
with open(fastq_links, 'r') as fastq:
for i, line in enumerate(fastq, 1):
# skip header
if not first:
line = line.strip()
try:
if not SEQ:
ref_name, fastq_path = line.split('\t')
else:
ref_name, ref_tube, bc_name, bs_seq, lane_id, num_reads, fq_path, fq_name = line.split(
'\t')
if input_parameters['test_call']:
fq_path = '%s/test_files/' % input_parameters[
'arq_path']
fastq_path = '%s%s' % (fq_path, fq_name)
except:
# print debug if an error occurs
if debug: printDebug(debug_list, format)
print(
"Error: Fastq links file \'%s\' is invalid in line \'%s\'! Can't read \'%s\'."
% (fastq_links, i, line))
sys.exit()
j = ('%s' % i) if not SEQ else ('%s' % (i - 1))
if debug: debug_list.append([ref_name, fastq_path, j])
# chech if fastq file exists
if not os.path.isfile("%s" % (fastq_path)):
if debug: printDebug(debug_list, format)
print(
"Error: No fastq file named \'%s\' in \'%s\' line \'%s\'!"
% (fastq_path, fastq_links, i))
sys.exit()
# save ref name and fastq link to dictionary
fastq_files[ref_name] = fastq_path
fastq_ref[j] = [ref_name, num_reads]
first = False
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return dictionary with fastq links and list with fastq data
return fastq_files, fastq_ref
def getQuantCombinations(job_requests, fastq_ref, input_parameters, debug):
# initialize debug
debug_list = []
format = '{:<15}'
if debug: debug_list.append(['def: getQuantCombinations():'])
if debug: debug_list.append(['-'])
if debug: debug_list.append(['combo', 'list'])
if debug: debug_list.append(['-'])
# initialize parameters
fastq_combos = {}
align_fq_comb = {}
align_jobs = []
quant_jobs = []
# get possible alignment and quantification jobs
for job in job_requests.keys():
if re.search(r'_align$', job):
align_jobs.append(re.sub(r'_align$', '', job))
if re.search(r'_quant$', job): quant_jobs.append(job)
# get possible fastq combinations
# transforms comb job list into
print("%s Status: Make fastq combinations ..." % (getTime()))
for combi_str in filter(None,
input_parameters['quant_comb_list'].split(';')):
fq_combi = ''
combi_list = []
for combi in sorted(filter(None, combi_str.split(','))):
# make fq combi key
fq_combi = '%s_%s' % (fq_combi, combi)
# save fastq names into ordered list (according to fq_combi) from -> fastq_ref[j] = [ref_name, num_reads]
combi_list.append(fastq_ref[combi][0])
# get final fq_combi entry into fastq_combos -> fastq_combos['_fq1_fq2_fq3'] = ['fq_name_1','fq_name_2','fq_name_3']
if debug: debug_list.append([fq_combi, combi_list])
fastq_combos[fq_combi] = combi_list
print("%s Combinations: %s" % (getTime(), fastq_combos.keys()))
# get possible aligner + fastq_combo combinations
if debug: debug_list.append(['-'])
print("%s Status: Make aligner-fastq combinations ..." % (getTime()))
for aligner in align_jobs:
for fq_combi in fastq_combos.keys():
aln_fq = '%s%s' % (aligner, fq_combi)
# get fastq - aligner combination -> align_fq_comb['ALIGNTOOL1_fq1_fq2_fq3'] = ['fq_name_1','fq_name_2','fq_name_3']
if debug: debug_list.append([aln_fq, fastq_combos[fq_combi]])
align_fq_comb[aln_fq] = fastq_combos[fq_combi]
print("%s Combinations: %s" % (getTime(), align_fq_comb.keys()))
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return
return align_fq_comb
def getArgsConfig(config, input_parameters, debug):
# initialize parameters
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: getArgsConfig():'])
if debug: debug_list.append(['-'])
if debug:
debug_list.append(
['config param', 'vaiable name', 'config data', 'terminal data'])
if debug: debug_list.append(['-'])
# read config file and validate parameters
with open(config, 'r') as cFile:
for i, line in enumerate(cFile, 1):
line = line.strip()
# skip comment lines
if not line or re.match(r'^\#', line):
continue
try:
conf_param, conf_data = line.split('\t')
except:
# print debug if an error occurs
if debug: printDebug(debug_list, format)
print(
"Error: Config file line \'%s\' is invalid! Can't read \'%s\'."
% (i, line))
sys.exit()
# get mandatory parameters
valid_conf_param = False
for param, data in input_parameters.iteritems():
# only save conf_data if data is empty
if conf_param.lower() == param:
if debug:
debug_list.append([conf_param, param, conf_data, data])
if not data: input_parameters[param] = conf_data
valid_conf_param = True
break
if not valid_conf_param:
# print debug if an error occurs
if debug: printDebug(debug_list, format)
print(
"Error: Parameter \'%s\' in your config file is not supported!"
% (conf_param))
sys.exit()
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return parameters
return input_parameters
def checkArgsProject(project, debug):
# initialize parameters
proj_dir_prefix = ''
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: checkArgsProject():'])
if debug: debug_list.append(['-'])
# check if project has a file path
proj_path = os.path.dirname(project)
proj_base = os.path.basename(project)
if debug: debug_list.append(['proj_path', proj_path])
if debug: debug_list.append(['proj_base', proj_base])
if not proj_base:
print("Error: Project name not defined properly!" % ())
sys.exit()
elif proj_path and not os.path.isdir(proj_path):
print("Error: \'%s\' has a path, but \'%s\' is not a directory!" %
(proj_base, proj_path))
sys.exit()
# ask user if this file path should be used as prefix
elif proj_path and os.path.isdir(proj_path):
print("%s Warning: \'%s\' has the path \'%s\'!" %
(getTime(), proj_base, proj_path))
print(
"%s Warning: This will overwrite all other \'PROJ_DIR_PREFIX\' variables."
% (getTime()))
user = userCall("Continue? y[es] / n[o]", True)
proj_dir_prefix = proj_path
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return arguments
return proj_dir_prefix, proj_base
def checkParametersDebug(input_parameters, job_requests, fastq1_files, fastq2_files, debug):
# initialize debug
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: main(): - parameters'])
if debug: debug_list.append(['-'])
if debug:
deb_print_list = {'input_param':input_parameters, 'job_requests':job_requests, 'fastq1_files':fastq1_files, 'fastq2_files':fastq2_files}
for j,name in enumerate(sorted(deb_print_list.keys()), 1):
for i,param in enumerate(sorted(deb_print_list[name].keys()), 1):
if i == 1: debug_list.append([name, '{\'%s\': \'%s\',' % (param, deb_print_list[name][param])])
elif i == len(deb_print_list[name].keys()): debug_list.append(['', ' \'%s\': \'%s\'}' % (param, deb_print_list[name][param])])
else: debug_list.append(['', ' \'%s\': \'%s\',' % (param, deb_print_list[name][param])])
if j < len(deb_print_list.keys()): debug_list.append(['-'])
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
def getJobRequests(possible_jobs, input_parameters, debug):
# initialize debug
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: getJobRequests():'])
if debug: debug_list.append(['-'])
# job dict
job_requests = {}
# top job type
job_base = input_parameters['only_make_job'].lower(
) if input_parameters['only_make_job'].lower() in [
'index', 'align', 'quant'
] else 'quant'
# get aligner and quantifier
if debug: debug_list.append(['job combi', 'job possible'])
if debug: debug_list.append(['-'])
for job_request in filter(None,
input_parameters['arq_job_list'].split(',')):
job_types = ['index', 'align', 'quant']
while job_base in job_types:
job_type = job_types.pop(0)
job_ext = '%s_%s' % (job_request.upper(), job_type)
job_possible = True if job_ext in possible_jobs.keys() else False
if debug: debug_list.append([job_ext, '%s' % job_possible])
if job_possible:
job_requests[job_ext] = ''
# get job dependencies
if debug: debug_list.append(['-'])
if debug: debug_list.append(['job', 'job_requests'])
if debug: debug_list.append(['-'])
for job_key in job_requests.keys():
job_requests = getDependency(possible_jobs, job_requests, job_key, [])
if debug: debug_list.append([job_key, job_requests.keys()])
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return all job requests
return job_requests
def checkArguments(input_parameters, debug):
# initialize parameters
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: checkArguments():'])
if debug: debug_list.append(['-'])
if debug: debug_list.append(['param', 'data'])
if debug: debug_list.append(['-'])
# check all parsed arguments in the input_parameters dictionary
for param, data in input_parameters.iteritems():
# save date if debug option is active
if debug: debug_list.append([param, data])
# check for files
if param in [
'fasta_gen_file', 'gtf_index_file', 'fastq1_links',
'fastq2_links'
]:
if not os.path.isfile("%s" % (data)):
if debug: printDebug(debug_list, format)
print("Error: No \'%s\' file named \'%s\'!" % (param, data))
sys.exit()
# check for tool directories
elif param and re.match(r'tool_dir_', param):
if not os.path.isdir("%s" % (data)):
if debug: printDebug(debug_list, format)
print("Error: No \'%s\' directory named \'%s\'!" %
(param, data))
sys.exit()
else:
''' TODO: check other arguments '''
pass
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
def makeMainDirectory(input_parameters, debug):
# initialize parameters
project_directory = input_parameters['proj_dir_path']
progress_data = []
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: makeMainDirectory():'])
if debug: debug_list.append(['-'])
# directory list
dir_list = ['index', 'align', 'quant', 'qsub', 'fastq', 'comb']
# save project directories
input_parameters['index_dir_path'] = '%s/indexes' % (project_directory)
input_parameters['align_dir_path'] = '%s/alignments/%s' % (
project_directory, input_parameters['data_set_name'])
input_parameters['quant_dir_path'] = '%s/quantifications/%s' % (
project_directory, input_parameters['data_set_name'])
input_parameters['qsub_dir_path'] = '%s/qsub_data' % (project_directory)
input_parameters['fastq_dir_path'] = '%s/fastq_data' % (project_directory)
input_parameters['comb_dir_path'] = '%s/comb_data/%s' % (
project_directory, input_parameters['data_set_name'])
# make new project folders
new_project = False
if not os.path.isdir(project_directory):
# main project folder
print("%s Status: Create \'%s\' main folder ..." %
(getTime(), input_parameters['p_name_suffix']))
os.mkdir(project_directory)
new_project = True
# make arq data directories
if not new_project:
print("%s Warning: \'%s\' already exists! Checking progress ..." %
(getTime(), project_directory))
# make data folders
if debug: debug_list.append(['dir key', 'dir path'])
if debug: debug_list.append(['-'])
for data_type in dir_list:
if not os.path.isdir(input_parameters['%s_dir_path' % data_type]):
print("%s Status: Create \'%s\' folder ..." %
(getTime(), data_type))
if debug:
debug_list.append([
'%s_dir_path' % data_type,
input_parameters['%s_dir_path' % data_type]
])
os.makedirs('%s' % (input_parameters['%s_dir_path' % data_type]))
else:
print("%s Warning: \'%s\' folder already exists ..." %
(getTime(), data_type))
# remove qsub error file if exists
if os.path.isfile('%s/qsub.error' % (input_parameters['qsub_dir_path'])):
print("%s Status: Remove \'qsub.error\' file ..." % (getTime()))
os.remove('%s/qsub.error' % (input_parameters['qsub_dir_path']))
# check tool progress
if not new_project:
print("%s Status: Check tool progress ..." % (getTime()))
for mainDirs in [
input_parameters['index_dir_path'],
input_parameters['align_dir_path'],
input_parameters['quant_dir_path']
]:
if debug: debug_list.append(['mainDirs', mainDirs])
# list all contents and check if content is a directory
for jobName in os.listdir(mainDirs):
subDirs = '%s/%s' % (mainDirs, jobName)
if debug: debug_list.append(['subDirs', subDirs])
if os.path.isdir(subDirs):
# save progress into progress_data
print("%s Warning: Job " % getTime() +
'{:<15}'.format("\'%s\'" %
(jobName)) + " ... is already done!")
progress_data.append(jobName)
if debug:
debug_list.append([
jobName,
True if jobName in progress_data else False
])
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return parameters
return input_parameters, progress_data
def makeToolDirectories(job_requests, input_parameters, job_type, debug):
# initialize debug
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: makeToolDirectories():'])
if debug: debug_list.append(['-'])
if debug: debug_list.append(['job', 'setting'])
if debug: debug_list.append(['-'])
# initiate tool job requests
job_list = {}
for job in job_requests.keys():
# save all tool jobs to job_list
if re.search(r'_%s$' % job_type, job):
job_list[job] = job_requests[job]
if debug: debug_list.append([job, job_list[job]])
# declare tool job folders
# job options: 'request', 'overwrite', 'skip', 'skipped', 'finished', 'required', 'ask', 'new'
if debug: debug_list.append(['-'])
for job in job_list.keys():
job_dir_name = '%s_dir' % job
job_folder = '%s/%s' % (input_parameters['%s_dir_path' % job_type],
job)
input_parameters[job_dir_name] = job_folder
if job_list[job] in ['request', 'overwrite', 'required', 'new']:
# check if it is an alignment job and ask if all alignments should be redone
if job_list[job] == 'overwrite' and job_type in ['align', 'quant']:
print(
"%s Warning: You requested \'overwrite\' for job \'%s\'!" %
(getTime(), job))
overwrite_all = userCall(
"Type: y[es] to remove all / n[o] to decide for each job part",
False)
job_list[job] = 'overwrite' if overwrite_all else 'ask'
# check for overwrite option and remove directory
if job_list[job] == 'overwrite':
removeJob(job_folder, [''], debug)
# make new folder
if not os.path.isdir(job_folder) and job_list[job] in [
'request', 'overwrite', 'new'
]:
print("%s Status: Create \'%s\' folder ..." % (getTime(), job))
os.mkdir(job_folder)
if job_list[job] == 'overwrite': job_list[job] = 'request'
if debug: debug_list.append([job, job_list[job]])
elif not os.path.isdir(job_folder) and job_list[job] == 'required':
if debug: printDebug(debug_list, format)
print("Error: \'%s\' is required but doesn't exist!" %
(job_folder))
sys.exit()
elif os.path.isdir(job_folder) and job_list[job] in [
'required', 'ask', 'new'
]:
pass
else:
if debug: printDebug(debug_list, format)
print("Error: \'%s\' already exists!" % (job_folder))
sys.exit()
elif job_list[job] == 'skip':
job_list[job] = 'skipped'
if debug: debug_list.append([job, job_list[job]])
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return index jobs
return job_list, input_parameters
def checkProgress(job_list, job_parts, input_parameters, job_type, debug):
# initialize debug
debug_list = []
format = '{:<15}'
if debug: debug_list.append(['def: checkProgress():'])
if debug: debug_list.append(['-'])
if debug: debug_list.append(['job', 'fastq', 'status', 'loop'])
if debug: debug_list.append(['-'])
# initialize parameters
todo_jobs = {}
job_parts_keys = []
quant_align_combi_list = input_parameters['quant_align_combi_list']
# make job list for fastq jobs
for job in job_list.keys():
# get job part keys
job_parts_keys = job_parts.keys()
# make modified job_list for quantification jobs
user_naming = ''
if job_type == 'quant':
job_parts_keys = []
for job_name in job_parts.keys():
if job in input_parameters['quant_name_list'].keys():
# add user defined name tag
user_naming = '_%s' % input_parameters['quant_name_list'][
job]
job_name = '%s%s' % (job_name, user_naming)
else:
user_naming = '_default'
# add 'default' name tag if no user defined name tag was given for this job
job_name = '%s%s' % (job_name, user_naming)
if ('%s_%s' %
(job.partition('_')[0],
job_name.partition('_')[0])) in quant_align_combi_list:
job_parts_keys.append(job_name)
# make dict for each job type
job_requests = {}
# check each job when 'ask' was requested
if job_list[job] in ['ask', 'new']:
# check existing tool and job part data and save it to todo_jobs dict
job_tool_dir = '%s/%s' % (input_parameters['%s_dir_path' %
job_type], job)
for jobName in os.listdir(job_tool_dir):
jobBaseName = os.path.basename(os.path.splitext(jobName)[0])
if debug:
debug_list.append([job, jobName, jobBaseName, 'basename'])
# only save existing jobs which are in the job part file list
if jobBaseName in job_parts_keys:
if job_type == 'quant':
jobBaseName = re.sub(r'\_[a-zA-Z0-9]+$', '',
jobBaseName)
job_requests['%s' % jobBaseName] = ''
if debug:
debug_list.append(
[job, jobName, jobBaseName, 'bname in keys'])
if debug: debug_list.append(['-'])
# iterate through requested job part names
for job_name in job_parts.keys():
if (job_type in [
'index', 'align'
]) or (job_type == 'quant' and
(('%s_%s' %
(job.partition('_')[0], job_name.partition('_')[0]))
in quant_align_combi_list)):
# job string
job_string = '%s/%s/%s%s' % (input_parameters[
'%s_dir_path' % job_type], job, job_name, user_naming)
# check if job was already done
if job_name in job_requests.keys(
) and job_list[job] == 'ask':
print(
"%s Warning: Job part \'%s%s\' for \'%s\' already exists!"
% (getTime(), job_name, user_naming, job))
overwrite = userCall(
"Type: y[es] to overwrite / n[o] to skip this job part",
False)
if overwrite:
removeJob(job_string, ['', '.sam', '.bam', '.ref'],
debug)
job_requests[job_name] = 'overwrite'
print(
"%s Status: Create \'%s%s\' folder for \'%s\' ..."
% (getTime(), job_name, user_naming, job))
os.mkdir(job_string)
else:
job_requests[job_name] = 'skip'
if debug:
debug_list.append([
job, job_name, job_requests[job_name],
'job exists'
])
elif job_name not in job_requests.keys():
job_requests[job_name] = 'request'
print(
"%s Status: Create \'%s%s\' folder for \'%s\' ..."
% (getTime(), job_name, user_naming, job))
os.mkdir(job_string)
if debug:
debug_list.append([
job, job_name, job_requests[job_name],
'new job'
])
else:
job_requests[job_name] = 'skipped'
if debug:
debug_list.append([
job, job_name, job_requests[job_name], 'else'
])
if debug: debug_list.append(['-'])
# save overwrite all and request jobs
elif job_list[job] in ['overwrite', 'request']:
for job_name in job_parts.keys():
if (job_type in [
'index', 'align'
]) or (job_type == 'quant' and
(('%s_%s' %
(job.partition('_')[0], job_name.partition('_')[0]))
in quant_align_combi_list)):
# job string
job_string = '%s/%s/%s%s' % (input_parameters[
'%s_dir_path' % job_type], job, job_name, user_naming)
print("%s Status: Create \'%s%s\' folder for \'%s\' ..." %
(getTime(), job_name, user_naming, job))
os.mkdir(job_string)
job_requests[job_name] = job_list[job]
if debug:
debug_list.append([
job, job_name, job_requests[job_name],
'overwrite or request'
])
# save to fastq jobs
if not job_list[job] == 'skipped':
todo_jobs[job] = job_requests
if debug: debug_list.append(['-'])
# add todo_jobs debug
if debug: debug_list.append(['-'])
if debug:
for job in todo_jobs.keys():
for part in todo_jobs[job]:
debug_list.append(
[job, part, todo_jobs[job][part], 'todo_jobs'])
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return fastq_jobs
return todo_jobs
def checkJobRequests(progress_data, job_requests, input_parameters, debug):
# initialize parameters
debug_list = []
format = '{:<15}'
if debug: debug_list.append(['def: checkJobRequests():'])
if debug: debug_list.append(['-'])
if debug:
debug_list.append(
['job', 'requests[job]', 'progress[job]', 'skip all', 'over all'])
if debug: debug_list.append(['-'])
# iterate through job requests and ask user to skip job if already done
do_for_all = {
'for_all_index': '',
'for_all_align': '',
'for_all_quant': '',
'for_all_new': ''
}
# skip -> skip all <TOOL> jobs
# overwrite -> overwrite all <TOOL> jobs (in case of alignment/quantification, it will be asked for every alignment/quantification)
# new -> make only jobs which aren't done yet
user_options = {
's': 'skip',
'skip': 'skip',
's all': 'skip',
'skip all': 'skip',
'o': 'overwrite',
'overwrite': 'overwrite',
'o all': 'overwrite',
'overwrite all': 'overwrite',
'n': 'new',
'new': 'new',
'n all': 'new',
'new all': 'new'
}
# make config setting list for user_set_over, user_set_skip and user_set_new
conf_settings = {}
options = ['over', 'skip', 'new']
for opt in options:
for job in filter(None,
input_parameters['user_set_%s' % opt].split(',')):
conf_settings[job] = opt[0]
# ask user
for job in job_requests.keys():
job_done = True if job in progress_data else False
# call if job was already done
if job_done and job == 'AFREE_align':
job_requests[job] = 'skip'
elif job_done:
all_job = 'for_all_%s' % job.rpartition('_')[2]
if job not in conf_settings.keys():
user_input = do_for_all[all_job] if do_for_all[
all_job] else raw_input(
"%s Warning: What to do with existing \'%s\' job \'%s\'? a[bort] / s[kip] / o[verwrite] / n[ew] [all]: "
% (getTime(), job[-5:], job))
else:
user_input = conf_settings[job]
input_valid = False
# only accept (case insensitive) as input
while not input_valid:
# handle do for all cases
if user_input.lower() in [
's all', 'skip all', 'o all', 'overwrite all', 'n all',
'new all'
]:
do_for_all[all_job] = user_options[user_input.lower()]
# do input command
if user_input.lower() in user_options.keys():
job_requests[job] = user_options[user_input.lower()]
input_valid = True
print("%s Status: Setting for job " % getTime() +
'{:<15}'.format("\'%s\'" % (job)) + " -> \'%s\'!" %
(job_requests[job]))
if debug:
debug_list.append([
job, job_requests[job], job_done, user_input,
do_for_all[all_job]
])
# abort if requested
elif user_input.lower() in ['a', 'abort']:
if debug: printDebug(debug_list, format)
print("%s Warning: Script interrupted by user ... " %
getTime())
sys.exit()
# repeat until input is valid
else:
user_input = raw_input(
"%s Warning: Wrong input! Use a[bort] / s[kip] / o[verwrite] / n[ew] [all]: "
% getTime())
# job not yet done
else:
job_requests[job] = 'request'
print("%s Status: Setting for job " % getTime() +
'{:<15}'.format("\'%s\'" %
(job)) + " -> \'%s\'!" % (job_requests[job]))
if debug:
debug_list.append([job, job_requests[job], job_done, '', ''])
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return updated job_requests
return job_requests
def getArguments(input_files, input_options, input_parameters, debug):
# initialize parameters
debug_list = []
format = '{:<17}'
if debug: debug_list.append(['def: getArguments():'])
if debug: debug_list.append(['-'])
if debug: debug_list.append(['key', 'value'])
if debug: debug_list.append(['-'])
# check project argument and save new project name if project had a path
input_parameters['proj_dir_prefix'], input_parameters[
'p_name_suffix'] = checkArgsProject(input_options['project'], debug)
if debug:
debug_list.append(['p_name_suffix', input_parameters['p_name_suffix']])
# check for command line input files and save if valid
''' TODO: do for input_options '''
for type, file in input_files.iteritems():
if file and os.path.isfile("%s" % (file)) and not type == 'config':
input_parameters[type] = file
if debug: debug_list.append([type, file])
elif file and not os.path.isfile("%s" % (file)):
print("Error: No \'%s\' file named \'%s\'!" % (type, file))
sys.exit()
# check command line prefix option and save if not already declared
prefix = input_options['prefix']
if prefix and not os.path.isdir(prefix):
print("Error: \'%s\' is not a directory!" % (prefix))
sys.exit()
elif prefix and not input_parameters['proj_dir_prefix']:
input_parameters['proj_dir_prefix'] = prefix
# get config arguments
print("%s Status: Save config parameters ..." % (getTime()))
input_parameters = getArgsConfig(input_files['config'], input_parameters,
debug)
if debug:
debug_list.append(
['proj_dir_prefix', input_parameters['proj_dir_prefix']])
# configure test data if test was requested
if input_parameters['test_call']:
input_parameters[
'proj_dir_prefix'] = '%s/test_files' % input_parameters['arq_path']
input_parameters[
'fasta_gen_file'] = '%s/test_files/EF204940.fa' % input_parameters[
'arq_path']
input_parameters[
'gtf_index_file'] = '%s/test_files/ef204940.gtf' % input_parameters[
'arq_path']
input_parameters[
'fastq1_links'] = '%s/test_files/arq_proj_test.fq1.info' % input_parameters[
'arq_path']
input_parameters[
'fastq2_links'] = '%s/test_files/arq_proj_test.fq2.info' % input_parameters[
'arq_path']
# define default parameters
''' TODO: make definition '''
# validate arguments
checkArguments(input_parameters, debug)
# define index base name without path
if not 'index_base_name' in input_parameters.keys():
input_parameters['index_base_name'] = os.path.basename(
os.path.splitext(input_parameters['fasta_gen_file'])[0])
if debug:
debug_list.append(
['index_base_name', input_parameters['index_base_name']])
# define tool directories
''' TODO: enable global tool command '''
# save and validate fastq files
print("%s Status: Save fastq files ..." % (getTime()))
''' TODO: make option for automatic fastq2 file read and enable 'paired end' option as input parameter '''
fastq1_files, fastq_ref = getArgsFastq(input_parameters['fastq1_links'],
input_parameters, debug)
if input_parameters['fastq2_links']:
fastq2_files, fastq_ref2 = getArgsFastq(
input_parameters['fastq2_links'], input_parameters, debug)
for fq_name in fastq1_files.keys():
if not ((re.sub(r'\_1.fq.gz$', '', fastq1_files[fq_name])
== re.sub(r'\_2.fq.gz$', '', fastq2_files[fq_name])) or
(re.sub(r'\-1.fastq.gz$', '', fastq1_files[fq_name])
== re.sub(r'\-2.fastq.gz$', '', fastq2_files[fq_name]))):
print(
"%s Warning: The links of \'%s\' are not equal after substituting the enumeration!"
% (getTime(), fq_name))
''' TODO: DO TEST '''
user = userCall("Continue? y[es] / n[o]", True)
# declare project folder
input_parameters['proj_dir_path'] = '%s/%s' % (
input_parameters['proj_dir_prefix'], input_parameters['p_name_suffix'])
if debug:
debug_list.append(['proj_dir_path', input_parameters['proj_dir_path']])
# print debug
if debug: printDebug(debug_list, format)
if debug: user = userCall("Continue? y[es] / n[o]", True)
# return arguments
return input_parameters, fastq1_files, fastq2_files, fastq_ref