def process_method(method, properties, body) -> dict:
metadata = json.loads(body.decode('utf-8'))
job_id = metadata['job_id']
app_logger.info('New Job Queued: {}'.format(job_id))
meta = read_data_from_s3(metadata['file_meta'], s3_bucket_name, index_column_first=False)
counts = read_data_from_s3(metadata['file_counts'], s3_bucket_name, index_column_first=True)
subsampler = Subsampler(bool(metadata['log']),
int(metadata['num_pc']),
int(metadata['num_cells']) if metadata.get('num_cells', False) else None
) if metadata.get('subsampling', False) else None
database_version = metadata.get('database_version', 'latest')
if database_version not in list_local_versions() + ['latest']:
database_version = 'latest'
app = cpdb_app.create_app(verbose=False, database_file=find_database_for(database_version))
if metadata['iterations']:
response = statistical_analysis(app, meta, counts, job_id, metadata, subsampler)
else:
response = non_statistical_analysis(app, meta, counts, job_id, metadata, subsampler)
return response
def test_non_statistical_method_subsampled_data_test__threshold__01__precision_3__num_pc_4__num_cells_4(self):
data = 'test_subsampled'
project_name = 'test_data'
threshold = 0.1
result_precision = 3
subsampler = Subsampler(False, 4, 4, debug_seed=0)
self._method_call(data, project_name, threshold, result_precision, subsampler)
def cpdb_method_analysis_launcher(
self,
raw_meta: pd.DataFrame,
counts: pd.DataFrame,
counts_data: str,
threshold: float,
result_precision: int,
subsampler: Subsampler = None,
) -> (pd.DataFrame, pd.DataFrame, pd.DataFrame):
if threshold < 0 or threshold > 1:
raise ThresholdValueException(threshold)
meta = method_preprocessors.meta_preprocessor(raw_meta)
counts = self._counts_validations(counts, meta)
if subsampler is not None:
counts = subsampler.subsample(counts)
meta = meta.filter(items=list(counts), axis=0)
interactions = self.database_manager.get_repository(
'interaction').get_all_expanded(include_gene=False)
genes = self.database_manager.get_repository('gene').get_all_expanded()
complex_composition = self.database_manager.get_repository(
'complex').get_all_compositions()
complex_expanded = self.database_manager.get_repository(
'complex').get_all_expanded()
means, significant_means, deconvoluted = cpdb_analysis_method.call(
meta, counts, counts_data, interactions, genes, complex_expanded,
complex_composition, self.separator, threshold, result_precision)
return means, significant_means, deconvoluted
def analysis(meta_filename: str,
counts_filename: str,
counts_data: str,
project_name: str,
threshold: float,
result_precision: int,
output_path: str,
output_format: str,
means_result_name: str,
significant_means_result_name: str,
deconvoluted_result_name: str,
verbose: bool,
database: Optional[str],
subsampling: bool,
subsampling_log: bool,
subsampling_num_pc: int,
subsampling_num_cells: Optional[int]
):
try:
subsampler = Subsampler(subsampling_log,
subsampling_num_pc,
subsampling_num_cells,
verbose) if subsampling else None
LocalMethodLauncher(cpdb_app.create_app(verbose,
database)).cpdb_analysis_local_method_launcher(meta_filename,
counts_filename,
counts_data,
project_name,
threshold,
output_path,
output_format,
means_result_name,
significant_means_result_name,
deconvoluted_result_name,
result_precision,
subsampler,
)
except (ReadFileException, ParseMetaException, ParseCountsException, ThresholdValueException,
AllCountsFilteredException) as e:
app_logger.error(str(e) +
(':' if (hasattr(e, 'description') and e.description) or (
hasattr(e, 'hint') and e.hint) else '') +
(' {}.'.format(e.description) if hasattr(e, 'description') and e.description else '') +
(' {}.'.format(e.hint) if hasattr(e, 'hint') and e.hint else '')
)
except EmptyResultException as e:
app_logger.warning(str(e) +
(':' if (hasattr(e, 'description') and e.description) or (
hasattr(e, 'hint') and e.hint) else '') +
(' {}.'.format(e.description) if hasattr(e, 'description') and e.description else '') +
(' {}.'.format(e.hint) if hasattr(e, 'hint') and e.hint else '')
)
except:
app_logger.error('Unexpected error')
if verbose:
traceback.print_exc(file=sys.stdout)
def analysis_scanpy(adata,
var_names,
obs_names,
obs_key,
var_key=None,
gene_id_format=None,
project_name='',
threshold=0.1,
result_precision='3',
output_path='',
output_format='csv',
means_result_name='means',
significant_means_result_name='significant_means',
deconvoluted_result_name='deconvoluted',
verbose=True,
database='latest',
subsampling=False,
subsampling_log=True,
subsampling_num_pc=100,
subsampling_num_cells=None,
write=False,
add_to_uns=True):
try:
subsampler = Subsampler(subsampling_log, subsampling_num_pc,
subsampling_num_cells,
verbose) if subsampling else None
out = LocalMethodLauncher(cpdb_app.create_app(
verbose, database)).cpdb_analysis_local_method_launcher_scanpy(
adata, var_names, obs_names, obs_key, var_key, gene_id_format,
project_name, threshold, output_path, output_format,
means_result_name, significant_means_result_name,
deconvoluted_result_name, result_precision, subsampler, write,
add_to_uns)
return out
except (ReadFileException, ParseMetaException, ParseCountsException,
ThresholdValueException, AllCountsFilteredException) as e:
app_logger.error(
str(e) + (':' if (hasattr(e, 'description') and e.description) or
(hasattr(e, 'hint') and e.hint) else '') +
(' {}.'.format(e.description)
if hasattr(e, 'description') and e.description else '') +
(' {}.'.format(e.hint) if hasattr(e, 'hint') and e.hint else ''))
except EmptyResultException as e:
app_logger.warning(
str(e) + (':' if (hasattr(e, 'description') and e.description) or
(hasattr(e, 'hint') and e.hint) else '') +
(' {}.'.format(e.description)
if hasattr(e, 'description') and e.description else '') +
(' {}.'.format(e.hint) if hasattr(e, 'hint') and e.hint else ''))
except:
app_logger.error('Unexpected error')
if verbose:
traceback.print_exc(file=sys.stdout)
def cpdb_statistical_analysis_launcher(
self,
raw_meta: pd.DataFrame,
counts: pd.DataFrame,
counts_data: str,
iterations: int,
threshold: float,
threads: int,
debug_seed: int,
result_precision: int,
pvalue: float,
subsampler: Subsampler = None,
) -> (pd.DataFrame, pd.DataFrame, pd.DataFrame, pd.DataFrame):
if threads < 1:
core_logger.info('Using Default thread number: %s' %
self.default_threads)
threads = self.default_threads
if threshold < 0 or threshold > 1:
raise ThresholdValueException(threshold)
meta = method_preprocessors.meta_preprocessor(raw_meta)
counts = self._counts_validations(counts, meta)
if subsampler is not None:
counts = subsampler.subsample(counts)
meta = meta.filter(items=(list(counts)), axis=0)
interactions = self.database_manager.get_repository(
'interaction').get_all_expanded(include_gene=False)
genes = self.database_manager.get_repository('gene').get_all_expanded()
complex_composition = self.database_manager.get_repository(
'complex').get_all_compositions()
complex_expanded = self.database_manager.get_repository(
'complex').get_all_expanded()
deconvoluted, means, pvalues, significant_means = \
cpdb_statistical_analysis_method.call(meta,
counts,
counts_data,
interactions,
genes,
complex_expanded,
complex_composition,
iterations,
threshold,
threads,
debug_seed,
result_precision,
pvalue,
self.separator)
return pvalues, means, significant_means, deconvoluted
def statistical_analysis(meta_filename: str,
counts_filename: str,
counts_data='ensembl',
project_name='',
threshold=0.1,
result_precision='3',
output_path='',
output_format='csv',
means_result_name='means',
significant_means_result_name='significant_means',
deconvoluted_result_name='deconvoluted',
verbose=True,
database='latest',
subsampling=False,
subsampling_log=True,
subsampling_num_pc=100,
subsampling_num_cells=None,
debug_seed='-1',
pvalue=0.05,
pvalues_result_name='pvalues',
iterations=1000,
threads=4) -> None:
database = choose_database(None, None, value=database)
try:
subsampler = Subsampler(subsampling_log, subsampling_num_pc,
subsampling_num_cells,
verbose) if subsampling else None
LocalMethodLauncher(cpdb_app.create_app(verbose, database)). \
cpdb_statistical_analysis_local_method_launcher(meta_filename,
counts_filename,
counts_data,
project_name,
iterations,
threshold,
output_path,
output_format,
means_result_name,
pvalues_result_name,
significant_means_result_name,
deconvoluted_result_name,
debug_seed,
threads,
result_precision,
pvalue,
subsampler,
)
except (ReadFileException, ParseMetaException, ParseCountsException,
ThresholdValueException, AllCountsFilteredException) as e:
app_logger.error(
str(e) + (':' if (hasattr(e, 'description') and e.description) or
(hasattr(e, 'hint') and e.hint) else '') +
(' {}.'.format(e.description)
if hasattr(e, 'description') and e.description else '') +
(' {}.'.format(e.hint) if hasattr(e, 'hint') and e.hint else ''))
except EmptyResultException as e:
app_logger.warning(
str(e) + (':' if (hasattr(e, 'description') and e.description) or
(hasattr(e, 'hint') and e.hint) else '') +
(' {}.'.format(e.description)
if hasattr(e, 'description') and e.description else '') +
(' {}.'.format(e.hint) if hasattr(e, 'hint') and e.hint else ''))
except:
app_logger.error('Unexpected error')
if verbose:
traceback.print_exc(file=sys.stdout)
