def setUp(self):
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
self.survey_job = survey_job
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value="DRR002116")
key_value_pair.save()
# Insert the organism into the database so the model doesn't call the
# taxonomy API to populate it.
organism = Organism(name="HOMO_SAPIENS",
taxonomy_id=9606,
is_scientific_name=True)
organism.save()
organism1 = Organism(name="GALLUS_GALLUS",
taxonomy_id=9031,
is_scientific_name=True)
organism1.save()
organism2 = Organism(name="DANIO_RERIO",
taxonomy_id=7955,
is_scientific_name=True)
organism2.save()
def test_survey_unmated_reads(self, mock_send_job):
"""Test an experiment with unmated reads.
Also make sure the file report endpoint's properties are recorded.
"""
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="experiment_accession_code", value="SRP048683"
)
key_value_pair.save()
sra_surveyor = SraSurveyor(survey_job)
experiment, samples = sra_surveyor.discover_experiment_and_samples()
self.assertEqual(experiment.accession_code, "SRP048683")
self.assertEqual(len(samples), 12)
expected_file_names = set()
# Just check one file for one sample's expected file size/md5
for sample in samples:
if sample.accession_code == "SRR1603661":
for original_file in sample.original_files.all():
expected_file_names.add(original_file.source_filename)
if original_file.source_filename == "SRR1603661_1.fastq.gz":
self.assertEqual(
original_file.expected_md5, "502a9a482bfa5aa75865ccc0105ad13c"
)
self.assertEqual(original_file.expected_size_in_bytes, 6751980628)
self.assertEqual({"SRR1603661_1.fastq.gz", "SRR1603661_2.fastq.gz"}, expected_file_names)
def test_calls_survey(self, mock_get):
"""If source_type is supported calls the appropriate survey method."""
mock_get.side_effect = mocked_requests_get
# Prevent a call being made to NCBI's API to determine
# organism name/id.
organism = Organism(name="H**O SAPIENS", taxonomy_id=9606, is_scientific_name=True)
organism.save()
survey_job = SurveyJob(source_type="ARRAY_EXPRESS")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value="E-GEOD-22166")
key_value_pair.save()
surveyor.run_job(survey_job)
logger.info("Started Survey Job %d, waiting for it to complete.", survey_job.id)
survey_job = wait_for_job(survey_job, SurveyJob)
self.assertTrue(survey_job.success)
batch = Batch.objects.all()[0]
batch = Batch.objects.filter(survey_job=survey_job).get()
downloader_job = batch.downloaderjob_set.get()
logger.info("Survey Job finished, waiting for Downloader Job %d to complete.",
downloader_job.id)
downloader_job = wait_for_job(downloader_job, DownloaderJob)
self.assertTrue(downloader_job.success)
processor_job = batch.processorjob_set.get()
logger.info("Downloader Job finished, waiting for processor Job %d to complete.",
processor_job.id)
processor_job = wait_for_job(processor_job, ProcessorJob)
self.assertTrue(processor_job.success)
def survey_ae_experiment(experiment_accession):
survey_job = SurveyJob(source_type="ARRAY_EXPRESS")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=experiment_accession)
key_value_pair.save()
run_job(survey_job)
def setUp(self):
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
self.survey_job = survey_job
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="EnsemblPlants")
key_value_pair.save()
def test():
survey_job = SurveyJob(source_type="ARRAY_EXPRESS")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value="E-MTAB-3050")
key_value_pair.save()
run_job(survey_job)
return
def prep_test(self, experiment_accession):
survey_job = SurveyJob(source_type="GEO")
survey_job.save()
self.survey_job = survey_job
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=experiment_accession)
key_value_pair.save()
def queue_surveyor_for_accession(accession: str) -> None:
"""Dispatches a surveyor job for the accession code."""
# Start at 256MB of RAM for surveyor jobs.
survey_job = SurveyJob(ram_amount=256)
set_source_type_for_accession(survey_job, accession)
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=accession)
key_value_pair.save()
def create_job_for_accession(self, accession_code: str):
survey_job = SurveyJob(source_type="ARRAY_EXPRESS")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=accession_code)
key_value_pair.save()
return survey_job
def test_survey_bacteria(self, mock_send_job):
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="EnsemblBacteria")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value="PSEUDOMONAS_AERUGINOSA")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
surveyor.survey(source_type="TRANSCRIPTOME_INDEX")
downloader_jobs = DownloaderJob.objects.order_by("id").all()
self.assertEqual(downloader_jobs.count(), 1)
send_job_calls = []
for downloader_job in downloader_jobs:
send_job_calls.append(
call(Downloaders.TRANSCRIPTOME_INDEX, downloader_job))
mock_send_job.assert_has_calls(send_job_calls)
# Make sure the organism object got created with the correct
# taxonomy id by making sure this doesn't raise an exception.
Organism.objects.get(name="PSEUDOMONAS_AERUGINOSA", taxonomy_id=287)
def test_single_plant(self):
""" Tests that the files returned actually exist.
Tests the Metazoa division instead of the main division.
"""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="EnsemblPlants")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value="Arabidopsis thaliana")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
files = surveyor.discover_species()[0]
for file in files:
urllib.request.urlopen(file.source_url)
# Make sure the organism object got created by making sure
# this doesn't raise an exception.
Organism.objects.get(name="ARABIDOPSIS_THALIANA")
def test_correct_index_location_metazoa(self):
""" Tests that the files returned actually exist.
Tests the Metazoa division instead of the main division.
"""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="EnsemblMetazoa")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value="Octopus bimaculoides")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
files = surveyor.discover_species()[0]
for file in files:
urllib.request.urlopen(file.source_url)
# Make sure the organism object got created by making sure
# this doesn't raise an exception.
Organism.objects.get(name="OCTOPUS_BIMACULOIDES")
def queue_surveyor_for_accession(accession: str) -> None:
"""Dispatches a surveyor job for the accession code."""
# Start at 1GB of RAM for surveyor jobs.
survey_job = SurveyJob(ram_amount=1024)
set_source_type_for_accession(survey_job, accession)
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=accession)
key_value_pair.save()
# We don't actually send the job here, we just create it.
# The foreman will pick it up and dispatch it when the time is appropriate.
return survey_job
def test_correct_index_location(self):
""" Tests that the files returned actually exist.
Uses an organism in the main division.
"""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="Ensembl")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value="Danio rerio")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
files = surveyor.discover_species()[0]
# Make sure the organism object got created by making sure
# this doesn't raise an exception.
Organism.objects.get(name="DANIO_RERIO")
for file in files:
urllib.request.urlopen(file.source_url)
def test_nonexistant_srp_survey(self):
"""Try surveying an accession that does not exist
"""
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="experiment_accession_code", value="ERP006216"
)
key_value_pair.save()
run_job(survey_job)
survey_job.refresh_from_db()
self.assertFalse(survey_job.success)
self.assertEqual(survey_job.failure_reason, "No experiment found.")
def setUp(self):
survey_job = SurveyJob(source_type="ARRAY_EXPRESS")
survey_job.save()
self.survey_job = survey_job
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value="E-MTAB-3050")
key_value_pair.save()
# Insert the organism into the database so the model doesn't call the
# taxonomy API to populate it.
organism = Organism(name="H**O SAPIENS",
taxonomy_id=9606,
is_scientific_name=True)
organism.save()
def test_arrayexpress_alternate_accession(self):
""" Make sure that ENA experiments correctly detect their ArrayExpress alternate accession
"""
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="experiment_accession_code", value="ERP108370"
)
key_value_pair.save()
sra_surveyor = SraSurveyor(survey_job)
experiment, _ = sra_surveyor.discover_experiment_and_samples()
self.assertEqual(experiment.accession_code, "ERP108370")
self.assertEqual(experiment.alternate_accession_code, "E-MTAB-6681")
def test_survey_fungi_none(self, mock_send_job):
"""When surveying fungi an organism_name must be supplied."""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="EnsemblFungi")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
surveyor.survey(source_type="TRANSCRIPTOME_INDEX")
downloader_jobs = DownloaderJob.objects.order_by("id").all()
self.assertEqual(downloader_jobs.count(), 0)
mock_send_job.assert_not_called()
def survey_experiment(experiment_accession: str, source_type: str):
"""Survey an experiment of type `source_type`.
Source type corresponds to one of the external sources we
support. It must be one of the following values:
* SRA
* GEO
* ARRAY_EXPRESS
"""
survey_job = SurveyJob(source_type=source_type)
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=experiment_accession)
key_value_pair.save()
run_job(survey_job)
return survey_job
def test_survey(self, mock_send_job):
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="ensembl_division", value="EnsemblPlants"
)
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
surveyor.survey(source_type="TRANSCRIPTOME_INDEX")
downloader_jobs = DownloaderJob.objects.order_by("id").all()
self.assertGreater(downloader_jobs.count(), 50)
send_job_calls = []
for downloader_job in downloader_jobs:
send_job_calls.append(call(Downloaders.TRANSCRIPTOME_INDEX, downloader_job))
mock_send_job.assert_has_calls(send_job_calls)
def survey_sra_experiments(start_accession, end_accession):
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="start_accession",
value=start_accession)
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="end_accession",
value=end_accession)
key_value_pair.save()
run_job(survey_job)
def test_batch_created(self, mock_get):
mock_get.side_effect = mocked_requests_get
# Use same run accession for the start and end of the range to
# achieve a length of 1
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="start_accession",
value=RUN_ACCESSION)
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="end_accession",
value=RUN_ACCESSION)
key_value_pair.save()
surveyor = SraSurveyor(survey_job)
self.assertTrue(surveyor.discover_batches())
# With only a single run accession there should only be a
# single batch.
self.assertEqual(len(surveyor.batches), 1)
batch = surveyor.batches[0]
self.assertEqual(batch.survey_job.id, survey_job.id)
self.assertEqual(batch.source_type, "SRA")
self.assertEqual(batch.pipeline_required, "SALMON")
self.assertEqual(batch.platform_accession_code, "IlluminaHiSeq2000")
self.assertEqual(batch.experiment_accession_code, "DRX001563")
self.assertEqual(batch.experiment_title,
("Illumina HiSeq 2000 sequencing; "
"Exp_Gg_HH16_1_embryo_mRNAseq"))
self.assertEqual(batch.status, "NEW")
self.assertEqual(batch.release_date, "2013-07-19")
self.assertEqual(batch.last_uploaded_date, "2017-08-11")
self.assertEqual(batch.organism_id, 9031)
self.assertEqual(batch.organism_name, "GALLUS GALLUS")
file = batch.files[0]
self.assertEqual(file.size_in_bytes, -1)
self.assertEqual(
file.download_url,
"ftp://ftp.sra.ebi.ac.uk/vol1/fastq/DRR002/DRR002116/DRR002116.fastq.gz"
) # noqa
self.assertEqual(file.raw_format, "fastq.gz")
self.assertEqual(file.processed_format, "tar.gz")
self.assertEqual(file.name, "DRR002116.fastq.gz")
self.assertEqual(file.internal_location, "IlluminaHiSeq2000/SALMON")
def create_survey_job(self):
job = SurveyJob(source_type="SRA",
nomad_job_id="SURVEYOR/dispatch-1528945054-e8eaf540",
num_retries=0,
success=None)
job.save()
sjkv = SurveyJobKeyValue()
sjkv.key = "experiment_accession_code"
sjkv.value = "RJ-1234-XYZ"
sjkv.survey_job = job
sjkv.save()
return job
def test_discover_batches(self, mock_generate_batch):
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="start_accession",
value="DRR012345")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="end_accession",
value="DRR012348")
key_value_pair.save()
sra_surveyor = SraSurveyor(survey_job)
sra_surveyor.discover_batches()
mock_generate_batch.assert_has_calls([
call("DRR012345"),
call("DRR012346"),
call("DRR012347"),
call("DRR012348")
])
def set_source_type_for_accession(survey_job, accession: str) -> None:
"""Type a surveyor based on accession structure"""
if 'GSE' in accession[:3]:
survey_job.source_type = "GEO"
survey_job.save()
return
elif 'E-' in accession[:2]:
survey_job.source_type = "ARRAY_EXPRESS"
survey_job.save()
return
elif " " in accession:
survey_job.source_type = "TRANSCRIPTOME_INDEX"
survey_job.save()
args = accession.split(",")
# Allow organism to be unspecified so we survey the entire division.
organism_name = args[0] if len(args[0]) > 0 else None
if len(args) > 1:
ensembl_division = args[1].strip()
else:
ensembl_division = "Ensembl"
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value=ensembl_division)
key_value_pair.save()
if organism_name:
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value=organism_name)
key_value_pair.save()
return
else:
survey_job.source_type = "SRA"
survey_job.save()
return
def test_correct_index_location_protist(self):
""" Tests that the files returned actually exist.
Tests the Metazoa division instead of the main division.
"""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="EnsemblProtists")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value="Leishmania major")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(survey_job)
files = surveyor.discover_species()[0]
for file in files:
urllib.request.urlopen(file.source_url)
def survey_transcriptome_index(organism_name=None, ensembl_division='Ensembl'):
"""Special one-off surveyor to build transcriptome indices.
The external source this uses is ensembl.org which is divided into
multiple divisions. This function surveys only one division at a
time. If an `organism_name` is provided, survey only that
organism, otherwise survey the entire division.
"""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value=ensembl_division)
key_value_pair.save()
if organism_name:
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value=organism_name)
key_value_pair.save()
run_job(survey_job)
return survey_job
def test_correct_index_location(self):
""" Tests that the files returned actually exist.
Uses an organism in the main division.
"""
survey_job = SurveyJob(source_type="TRANSCRIPTOME_INDEX")
survey_job.save()
self.survey_job = survey_job
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="ensembl_division",
value="Ensembl")
key_value_pair.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="organism_name",
value="Danio rerio")
key_value_pair.save()
surveyor = TranscriptomeIndexSurveyor(self.survey_job)
files = surveyor.discover_species()[0]
for file in files:
urllib.request.urlopen(file.source_url)
def test_srp_survey(self, mock_send_job):
"""A slightly harder test of the SRA surveyor.
"""
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="experiment_accession_code", value="SRP068364"
)
key_value_pair.save()
sra_surveyor = SraSurveyor(survey_job)
experiment, samples = sra_surveyor.discover_experiment_and_samples()
self.assertEqual(experiment.accession_code, "SRP068364")
self.assertEqual(experiment.alternate_accession_code, "GSE76780")
self.assertEqual(len(samples), 4)
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="experiment_accession_code", value="SRP111553"
)
key_value_pair.save()
sra_surveyor = SraSurveyor(survey_job)
experiment, samples = sra_surveyor.discover_experiment_and_samples()
self.assertEqual(experiment.accession_code, "SRP111553")
self.assertEqual(experiment.alternate_accession_code, "GSE101204")
self.assertEqual(len(samples), 16) # 8 samples with 2 runs each
survey_job = SurveyJob(source_type="SRA")
survey_job.save()
key_value_pair = SurveyJobKeyValue(
survey_job=survey_job, key="experiment_accession_code", value="DRP003977"
)
key_value_pair.save()
sra_surveyor = SraSurveyor(survey_job)
experiment, samples = sra_surveyor.discover_experiment_and_samples()
self.assertEqual(experiment.accession_code, "DRP003977")
self.assertEqual(experiment.alternate_accession_code, None)
self.assertEqual(len(samples), 9)
def test_geo_survey_microarray(self, mock_send_task):
"""Test that the unsurveyor works correctly.
This includes not deleting samples which also belong to other
experiments. Therefore we survey a superseries and one of its
sub-experiments, then delete the superseries to make sure the
sub-experiment wasn't touched.
We mock out the send_job function so that we don't actually
process these. The unsurveyor code related to ComputedFile,
ComputationalResult, and ProcessorJobs won't be tested by
this, but it's been functionally tested.
"""
superseries_accession = "GSE59795"
sub_experiment_accession = "GSE46580"
# Survey the superseries.
survey_job = SurveyJob(source_type="GEO")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=superseries_accession)
key_value_pair.save()
geo_surveyor = GeoSurveyor(survey_job)
geo_surveyor.survey()
# Survey the sub-experiment
survey_job = SurveyJob(source_type="GEO")
survey_job.save()
key_value_pair = SurveyJobKeyValue(survey_job=survey_job,
key="experiment_accession_code",
value=sub_experiment_accession)
key_value_pair.save()
geo_surveyor = GeoSurveyor(survey_job)
geo_surveyor.survey()
# Establish baselines before purge
experiment = Experiment.objects.filter(accession_code=sub_experiment_accession)[0]
experiment_sample_assocs = ExperimentSampleAssociation.objects.filter(experiment=experiment)
samples = Sample.objects.filter(id__in=experiment_sample_assocs.values('sample_id'))
self.assertEqual(samples.count(), 4)
og_file_sample_assocs = OriginalFileSampleAssociation.objects.filter(sample_id__in=samples.values('id'))
original_files = OriginalFile.objects.filter(id__in=og_file_sample_assocs.values('original_file_id'))
self.assertEqual(original_files.count(), 4)
experiment = Experiment.objects.filter(accession_code=superseries_accession)[0]
experiment_sample_assocs = ExperimentSampleAssociation.objects.filter(experiment=experiment)
samples = Sample.objects.filter(id__in=experiment_sample_assocs.values('sample_id'))
self.assertEqual(samples.count(), 20)
og_file_sample_assocs = OriginalFileSampleAssociation.objects.filter(sample_id__in=samples.values('id'))
original_files = OriginalFile.objects.filter(id__in=og_file_sample_assocs.values('original_file_id'))
self.assertEqual(original_files.count(), 20)
# Purge the superseries
purge_experiment(superseries_accession)
# Make sure the subexperiment samples weren't affected.
experiment = Experiment.objects.filter(accession_code=sub_experiment_accession)[0]
experiment_sample_assocs = ExperimentSampleAssociation.objects.filter(experiment=experiment)
samples = Sample.objects.filter(id__in=experiment_sample_assocs.values('sample_id'))
self.assertEqual(samples.count(), 4)
# Make sure sub-experiment original files weren't affected.
og_file_sample_assocs = OriginalFileSampleAssociation.objects.filter(sample_id__in=samples.values('id'))
original_files = OriginalFile.objects.filter(id__in=og_file_sample_assocs.values('original_file_id'))
self.assertEqual(original_files.count(), 4)
# And that samples and files that remain are from the subseries.
self.assertEqual(Sample.objects.count(), 4)
self.assertEqual(OriginalFile.objects.count(), 4)
