def test_get_topology(self):
ncbi = NCBITaxa(dbfile=DATABASE_PATH)
t1 = ncbi.get_topology([9606, 7507, 9604])
t2 = ncbi.get_topology([9606, 7507, 678])
self.assertEqual(sorted(t1.get_leaf_names()), ["7507", "9606"])
self.assertEqual(sorted(t2.get_leaf_names()), ["678", "7507", "9606"])
def get_desired_ranks(taxid, desired_ranks):
ncbi = NCBITaxa()
lineage = ncbi.get_lineage(taxid)
names = ncbi.get_taxid_translator(lineage)
lineage2ranks = ncbi.get_rank(names)
ranks2lineage = dict(
(rank, taxid) for (taxid, rank) in lineage2ranks.items())
return [ranks2lineage.get(rank, '0') for rank in desired_ranks]
def annotate_ncbi_taxa(self,
taxid_attr='species',
tax2name=None,
tax2track=None,
tax2rank=None,
dbfile=None):
"""Add NCBI taxonomy annotation to all descendant nodes. Leaf nodes are
expected to contain a feature (name, by default) encoding a valid taxid
number.
All descendant nodes (including internal nodes) are annotated with the
following new features:
`Node.spname`: scientific spcies name as encoded in the NCBI taxonomy database
`Node.named_lineage`: the NCBI lineage track using scientific names
`Node.taxid`: NCBI taxid number
`Node.lineage`: same as named_lineage but using taxid codes.
Note that for internal nodes, NCBI information will refer to the first
common lineage of the grouped species.
:param name taxid_attr: the name of the feature that should be used to access the taxid number associated to each node.
:param None tax2name: A dictionary where keys are taxid numbers and
values are their translation into NCBI scientific name. Its use is
optional and allows to avoid database queries when annotating many trees
containing the same set of taxids.
:param None tax2track: A dictionary where keys are taxid numbers and
values are their translation into NCBI lineage tracks (taxids). Its use is
optional and allows to avoid database queries when annotating many trees
containing the same set of taxids.
:param None tax2rank: A dictionary where keys are taxid numbers and
values are their translation into NCBI rank name. Its use is optional
and allows to avoid database queries when annotating many trees
containing the same set of taxids.
:param None dbfile : If provided, the provided file will be used as a
local copy of the NCBI taxonomy database.
:returns: tax2name (a dictionary translating taxid numbers into
scientific name), tax2lineage (a dictionary translating taxid numbers
into their corresponding NCBI lineage track) and tax2rank (a dictionary translating taxid numbers into
rank names).
"""
ncbi = NCBITaxa(dbfile=dbfile)
return ncbi.annotate_tree(self,
taxid_attr=taxid_attr,
tax2name=tax2name,
tax2track=tax2track,
tax2rank=tax2rank)
def get_family(taxid):
ncbi = NCBITaxa()
lineage = ncbi.get_lineage(taxid)
ranks = ncbi.get_rank(lineage)
for rank in ranks.keys():
if ranks[rank] == 'family':
return rank
return "None"
def get_rank(self, rank_level):
ncbi = NCBITaxa()
lineage = ncbi.get_lineage(self.tax_id)
ranks = ncbi.get_rank(lineage)
for rank in ranks.keys():
if ranks[rank] == rank_level:
return rank
return "N/A"
def get_rank(taxid, rank_level):
ncbi = NCBITaxa()
lineage = ncbi.get_lineage(taxid)
ranks = ncbi.get_rank(lineage)
for rank in ranks.keys():
if ranks[rank] == rank_level:
return rank
return "None"
def test_ncbiquery(self):
ncbi = NCBITaxa(dbfile=DATABASE_PATH)
id2name = ncbi.get_taxid_translator(["9606", "7507"])
self.assertEqual(id2name[7507], "Mantis religiosa")
self.assertEqual(id2name[9606], "H**o sapiens")
name2id = ncbi.get_name_translator(["Mantis religiosa", "h**o sapiens"])
self.assertEqual(name2id["Mantis religiosa"], 7507)
self.assertEqual(name2id["h**o sapiens"], 9606)
def taxo_msa(outfile='taxo_msa.svg',taxids=[],annotation='',msa=[],title='',width=2000):
"""
Visualize MSA together with a taxonomy tree
taxids - list of taxids in the same order as seqs in msa
"""
# taxid2gi={f_df.loc[f_df.gi==int(gi),'taxid'].values[0]:gi for gi in list(f_df['gi'])}
# gi2variant={gi:f_df.loc[f_df.gi==int(gi),'hist_var'].values[0] for gi in list(f_df['gi'])}
# msa_dict={i.id:i.seq for i in msa_tr}
ncbi = NCBITaxa()
taxids=map(int,taxids)
t = ncbi.get_topology(taxids,intermediate_nodes=False)
a=t.add_child(name='annotation')
a.add_feature('sci_name','annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if(node.rank in ['order','class','phylum','kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name=='annotation':
s=str(msa[taxids.index(int(node.name))].seq)
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
# gi=taxid2gi[int(node.name)]
add_face_to_node(TextFace(' '+msa[taxids.index(int(node.name))].id),node,column=1, position = "aligned")
# add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
if node.is_leaf() and node.name=='annotation':
if(annotation):
s=annotation
# get_hist_ss_in_aln_as_string(msa_tr)
else:
s=' '*len(msa[0].seq)
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
add_face_to_node(TextFace(' '+'SEQ_ID'),node,column=1, position = "aligned")
# add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render(outfile, w=width, dpi=300, tree_style=ts)
def getNcbiTaxonomy():
ncbi = NCBITaxa()
nameToTaxIdList = ncbi.get_name_translator(ORGANISM_NAMES_LIST)
#print (str(nameToTaxIdList))
with open (OUTPUT_FILE, "w") as outputFile:
for name in ORGANISM_NAMES_LIST:
#for name, taxIds in nameToTaxIdList.items():
taxIds = nameToTaxIdList[name]
for eachId in taxIds:
lineage = ncbi.get_lineage(str(eachId))
names = ncbi.get_taxid_translator(lineage)
outputFile.write("\t".join([names[taxid] for taxid in lineage]) + "\n")
def annotate_ncbi_taxa(self, taxid_attr="species", tax2name=None, tax2track=None, tax2rank=None, dbfile=None):
"""Add NCBI taxonomy annotation to all descendant nodes. Leaf nodes are
expected to contain a feature (name, by default) encoding a valid taxid
number.
All descendant nodes (including internal nodes) are annotated with the
following new features:
`Node.spname`: scientific spcies name as encoded in the NCBI taxonomy database
`Node.named_lineage`: the NCBI lineage track using scientific names
`Node.taxid`: NCBI taxid number
`Node.lineage`: same as named_lineage but using taxid codes.
Note that for internal nodes, NCBI information will refer to the first
common lineage of the grouped species.
:param name taxid_attr: the name of the feature that should be used to access the taxid number associated to each node.
:param None tax2name: A dictionary where keys are taxid numbers and
values are their translation into NCBI scientific name. Its use is
optional and allows to avoid database queries when annotating many trees
containing the same set of taxids.
:param None tax2track: A dictionary where keys are taxid numbers and
values are their translation into NCBI lineage tracks (taxids). Its use is
optional and allows to avoid database queries when annotating many trees
containing the same set of taxids.
:param None tax2rank: A dictionary where keys are taxid numbers and
values are their translation into NCBI rank name. Its use is optional
and allows to avoid database queries when annotating many trees
containing the same set of taxids.
:param None dbfile : If provided, the provided file will be used as a
local copy of the NCBI taxonomy database.
:returns: tax2name (a dictionary translating taxid numbers into
scientific name), tax2lineage (a dictionary translating taxid numbers
into their corresponding NCBI lineage track) and tax2rank (a dictionary translating taxid numbers into
rank names).
"""
ncbi = NCBITaxa(dbfile=dbfile)
return ncbi.annotate_tree(
self, taxid_attr=taxid_attr, tax2name=tax2name, tax2track=tax2track, tax2rank=tax2rank
)
def ncbi_compare(self, autodetect_duplications=True, cached_content=None):
if not cached_content:
cached_content = self.get_cached_content()
cached_species = set([n.species for n in cached_content[self]])
if len(cached_species) != len(cached_content[self]):
print cached_species
ntrees, ndups, target_trees = self.get_speciation_trees(
autodetect_duplications=autodetect_duplications,
map_features=["taxid"])
else:
target_trees = [self]
ncbi = NCBITaxa()
for t in target_trees:
ncbi.get_broken_branches(t, cached_content)
def ncbi_compare(self, autodetect_duplications=True, cached_content=None):
if not cached_content:
cached_content = self.get_cached_content()
cached_species = set([n.species for n in cached_content[self]])
if len(cached_species) != len(cached_content[self]):
print cached_species
ntrees, ndups, target_trees = self.get_speciation_trees(
autodetect_duplications=autodetect_duplications, map_features=["taxid"]
)
else:
target_trees = [self]
ncbi = NCBITaxa()
for t in target_trees:
ncbi.get_broken_branches(t, cached_content)
def test_ncbiquery(self):
ncbi = NCBITaxa(dbfile=DATABASE_PATH)
id2name = ncbi.get_taxid_translator(['9606', '7507'])
self.assertEqual(id2name[7507], 'Mantis religiosa')
self.assertEqual(id2name[9606], 'H**o sapiens')
name2id = ncbi.get_name_translator(['Mantis religiosa', 'h**o sapiens'])
self.assertEqual(name2id['Mantis religiosa'], [7507])
self.assertEqual(name2id['h**o sapiens'], [9606])
name2id = ncbi.get_name_translator(['Bacteria'])
self.assertEqual(set(name2id['Bacteria']), set([2, 629395]))
out = ncbi.get_descendant_taxa("9605", intermediate_nodes=True)
#Out[9]: [1425170, 741158, 63221, 9606]
self.assertEqual(set(out), set([1425170, 741158, 63221, 9606]))
out = ncbi.get_descendant_taxa("9605", intermediate_nodes=False)
#Out[10]: [1425170, 741158, 63221]
self.assertEqual(set(out), set([1425170, 741158, 63221]))
out = ncbi.get_descendant_taxa("9605", intermediate_nodes=False, rank_limit="species")
#Out[11]: [9606, 1425170]
self.assertEqual(set(out), set([9606, 1425170]))
def bio_tree(names):
from collections import OrderedDict
from Bio import Entrez
from ete2 import NCBITaxa, PhyloTree
from lxml import etree
Entrez.email = '*****@*****.**'
ncbi = NCBITaxa()
ids = []
for name in names:
handle = Entrez.esearch(db='taxonomy', term=name)
while True:
line = handle.readline()
if not line: break
if '<Id>' in line:
ids.append(int(line.strip('<Id></Id>\n')))
scientific_tree = ncbi.get_topology(ids)
return scientific_tree.get_ascii(attributes=['sci_name'])
def get_desired_ranks(taxid, desired_ranks):
""" Gets the parent TaxID for a particular Taxon node.
Args:
taxid: Taxon Node whose parent TaxID at a given level needs
to be determined
desired_ranks: Parent Taxon level at which Taxon ID must be
determined.
Returns:
List of TaxIDs at particular taxon level
"""
ncbi = NCBITaxa()
lineage = ncbi.get_lineage(taxid)
names = ncbi.get_taxid_translator(lineage)
lineage2ranks = ncbi.get_rank(names)
ranks2lineage = dict(
(rank, taxid) for (taxid, rank) in lineage2ranks.items())
return [ranks2lineage.get(rank, '0') for rank in desired_ranks]
def parseVSearchOutputAgainstNCBI(vsearch_out, database, output_file,
min_coverage, min_similarity):
"""Resolves vsearch matches in a vsearch output file to the taxonomic name taken from BOLD.
Takes in a vsearch output file from usearch__global, parses the result for good matches, and
writes an output file mapping sequence name to taxa name.
:param vsearch_out: An output file from vsearch's usearch__global program.
:param database: The database used as part of the vsearch usearch__global operation.
:param output_file: Where to write the resulting file that maps sequence ID to taxanomic name.
:param min_coverage: The minimum coverage for an acceptable vsearch match.
:param min_similarity: The minimum simmilarity for an acceptable vsearch match.
"""
min_simm = float(min_similarity)
min_coverage = float(min_coverage)
ncbi = NCBITaxa()
conn = sqlite3.connect(database)
c = conn.cursor()
query = "select taxid from gi_taxid where gi=%s"
def getTaxFromId(taxId,
taxonomy=[
"species", "genus", 'family', 'order', 'class',
'phylum'
]):
myTaxonomy = dict([(a, "") for a in taxonomy])
taxId = int(taxId)
for lin in ncbi.get_lineage(taxId):
rank = ncbi.get_rank([lin]).values()[0]
if rank in taxonomy:
val = ncbi.get_taxid_translator([lin]).values()[0]
myTaxonomy[rank] = val
return ":".join([myTaxonomy[x] for x in taxonomy[::-1]])
with open(output_file, 'w') as out:
for line in open(vsearch_out, 'r'):
data = line.split()
if float(data[4]) > min_coverage or float(data[2]) > min_simm:
hit = c.execute(query % data[1]).fetchone()
if hit:
taxonomy = getTaxFromId(hit[0])
data.append(taxonomy)
printVerbose("\t".join(data))
out.write("\t".join(data))
out.write("\n")
else:
printErrorMissingID(out, data[1])
def __init__(self, data_path, workbench=None, genomes=[], taxDb=None):
self.data_path = data_path
self.workbench = workbench
self.metadata_path = pjoin(self.data_path, "metadata")
if not os.path.exists(self.metadata_path):
os.makedirs(self.metadata_path)
self.metadata_file = pjoin(self.metadata_path, "metadata.csv")
if taxDb:
self.taxDb = taxDb
else:
self.taxDb = NCBITaxa()
self.genomes = genomes
def main():
args = parser.parse_args()
cazy_fp = args.cazy_fp
p2taxid_fp = args.p2taxid_fp
output_fp = args.output_fp
len_fp = args.len_fp
ncbi = NCBITaxa()
# read in ncbi prot to taxid, keep in memory as a dict
prot_to_taxid = read_NCBI_prot_to_taxid_gz(p2taxid_fp)
# open
hmm_len = read_hmm_len_fp(len_fp)
# read in cazy
cazy_f = fasta_iter(cazy_fp)
# for each cazy,
with open(output_fp, 'w') as f:
for header, seq in cazy_f:
acc = header.split('|')[0]
fam = header.split('|')[1]
try:
gene_length = hmm_len[fam]
except KeyError:
gene_length = 1000
try:
taxid = prot_to_taxid[acc]
taxonomy = '.'.join(get_taxon_path(taxid, ncbi))
except KeyError:
taxonomy = 'unclassified'
outline = '{0}\t{1}\t{2}\t{3}\n'.format(header, fam, gene_length,
taxonomy)
f.write(outline)
def blast2summary_dict(db, blastpath): # (Path, Path) -> list[dict]
"""Reading in a blast output file, lookup all seqids to get taxids with a single blastdbcmd.
Then, lookup the taxonomy using ETE2 via the taxid, and add that info to the blast info."""
rows = csv.DictReader(open(blastpath),
delimiter='\t',
fieldnames=[
'qseqid', 'sseqid', 'pid', 'alnlen', 'gapopen',
'qstart', 'qend', 'sstart', 'send', 'evalue',
'bitscore'
])
rows = list(rows)
seqids = map(get('sseqid'), rows)
taxids = get_taxid(db, seqids)
gis = (s.split('|')[1] for s in seqids)
matches = dict(
(taxids[gi], row) for gi, row in zip(gis, rows) if gi in taxids)
ncbi = NCBITaxa(
) # downloads database and creates SQLite database if needed
return dictmap(lambda tid, row: merge(row, taxonomy(ncbi, tid)), matches)
def check_taxa_db_age(dbLocation):
# if file doesn't exist, catch the error and run the update, as it will create the file.
ncbi = NCBITaxa()
try:
filetime = datetime.fromtimestamp(path.getctime(dbLocation))
one_month_ago = datetime.now() - timedelta(days=30)
if filetime < one_month_ago:
# File older than 1 month, update it:
logInfo = '<> NCBITaxa Database older than 1 month, updating it <>'
ncbi.update_taxonomy_database()
else:
logInfo = '<> NCBITaxa Database up to date <>'
except:
logInfo = "<> NCBITaxa Database didn't exist, downloaded it <>"
ncbi.update_taxonomy_database()
return(logInfo)
def blast2summary_dict(db, blastpath, ete2_db): # (Path, Path) -> list[dict]
"""Reading in a blast output file, lookup all seqids to get taxids with a single blastdbcmd.
Then, lookup the taxonomy using ETE2 via the taxid, and add that info to the blast info."""
# rows = csv.DictReader(open(blastpath), delimiter='\t',fieldnames=[SEQID, 'sseqid','pid', 'alnlen','gapopen','qstart','qend','sstart','send','evalue','bitscore'])
rows = csv.DictReader(open(blastpath),
delimiter='\t',
fieldnames=blast_columns)
rows = list(rows)
seqids = map(get('sseqid'), rows)
taxids = get_taxid(db, seqids)
def get_gi(s):
fields = s.split('|')
if len(fields) > 1:
return fields[1]
else:
raise ValueError("Seq ID %s is missing GI fields and '|'" % s)
gis = imap(get_gi, seqids)
#TODO: change matches to use something unique--not the TAXID! actually, why is it a dict
# in the first place? it should be a list of dictionaries, and then map over
# the dictionaries to merge them with the taxonomy info
# this will replace the lines:
# matches = . . .
# items = . . .
#matches = dict((taxids[gi], row) for gi, row in zip(gis,rows) if gi in taxids)
ncbi = NCBITaxa(
ete2_db) # downloads database and creates SQLite database if needed
# items = dictmap(lambda tid,row: merge(row, taxonomy(ncbi, tid)), matches)
matches = [
assoc(row, 'taxid', taxids[gi]) for gi, row in zip(gis, rows)
if gi in taxids
]
items = [merge(row1, taxonomy(ncbi, row1['taxid'])) for row1 in matches]
res = imap(partial(keyfilter, csv_fields.__contains__), items)
return res
#ete2 module
ete2_error = '''
ERROR: This program requires the Python module ete2.
Please install it and try running the program again.
'''
try:
from ete2 import NCBITaxa
except:
print ete2_error
sys.exit(0)
from rpy2.robjects.packages import importr
print 'Loading NCBI taxonomic data...'
print
ncbi= NCBITaxa()
#------------------------------------------------
# Checking/installing/loading ontoCAT (BioconductoR) and PSI-MI
#------------------------------------------------
# ontoCAT installed here as variable for later use
try:
ontoCAT = importr('ontoCAT')
except RRuntimeError:
print '''
This program requires ontoCAT (BioconductoR package) to run.
Currently installing ontoCat to your machine...
def run(args):
# add lineage profiles/stats
import re
from ete2 import PhyloTree, NCBITaxa
if not args.taxonomy and not args.info:
args.taxonomy = True
ncbi = NCBITaxa()
all_taxids = {}
all_names = set()
queries = []
if not args.search:
log.error('Search terms should be provided (i.e. --search) ')
sys.exit(-1)
for n in args.search:
queries.append(n)
try:
all_taxids[int(n)] = None
except ValueError:
all_names.add(n.strip())
# translate names
name2tax = ncbi.get_name_translator(all_names)
all_taxids.update([(v, None) for v in name2tax.values()])
not_found_names = all_names - set(name2tax.keys())
if args.fuzzy and not_found_names:
log.warn("%s unknown names", len(not_found_names))
for name in not_found_names:
# enable extension loading
tax, realname, sim = ncbi.get_fuzzy_name_translation(
name, args.fuzzy)
if tax:
all_taxids[tax] = None
name2tax[name] = tax
name2realname[name] = realname
name2score[name] = "Fuzzy:%0.2f" % sim
if args.taxonomy:
log.info("Dumping NCBI taxonomy of %d taxa..." % (len(all_taxids)))
t = ncbi.get_topology(all_taxids.keys(),
intermediate_nodes=args.full_lineage,
rank_limit=args.rank_limit,
collapse_subspecies=args.collapse_subspecies)
id2name = ncbi.get_taxid_translator([n.name for n in t.traverse()])
for n in t.traverse():
n.add_features(taxid=n.name)
n.add_features(sci_name=str(id2name.get(int(n.name), "?")))
n.name = "%s - %s" % (id2name.get(int(n.name), n.name), n.name)
lineage = ncbi.get_lineage(n.taxid)
n.add_features(
named_lineage='|'.join(ncbi.translate_to_names(lineage)))
dump(t,
features=[
"taxid", "name", "rank", "bgcolor", "sci_name",
"collapse_subspecies", "named_lineage"
])
elif args.info:
print '# ' + '\t'.join(
["Taxid", "Sci.Name", "Rank", "Named Lineage", "Taxid Lineage"])
translator = ncbi.get_taxid_translator(all_taxids)
ranks = ncbi.get_rank(all_taxids)
for taxid, name in translator.iteritems():
lineage = ncbi.get_lineage(taxid)
named_lineage = ','.join(ncbi.translate_to_names(lineage))
lineage_string = ','.join(map(str, lineage))
print '\t'.join([
str(taxid), name,
ranks.get(taxid, ''), named_lineage, lineage_string
])
import sys
import os
import urllib2
import gzip
import biom
import shutil
from numpy import random as np_rand
from ete2 import NCBITaxa
from scripts.loggingwrapper import LoggingWrapper as logger
try:
from configparser import ConfigParser
except ImportError:
from ConfigParser import ConfigParser
ncbi = NCBITaxa()
RANKS = ['species', 'genus', 'family', 'order', 'class', 'phylum', 'superkingdom']
MAX_RANK = 'family'
_log = None
"""
Reads a BIOM file and creates map of OTU: lineage, abundance
BIOM file format needs to have a taxonomy field in metadata which contains the taxonomy in the format:
RANK__SCINAME; LOWERRANK_LOWERSCINAME
"""
def read_taxonomic_profile(biom_profile, config, no_samples = None):
table = biom.load_table(biom_profile)
ids = table.ids(axis="observation")
samples = table.ids()
if no_samples is None:
no_samples = len(samples)
#This function is DEPRECATED
def check_tax_id_clade(clade_top_tax_id,check_tax_id):
"""Checks if given tax_id is inside a clade formed by taxa described by its top taxid"""
p=pd.read_table(PATH_to_NCBI_nodes_dmp,sep='|',usecols=[0,1],header=None)
G=nx.DiGraph()
#Load all taxonomy as graph
G.add_edges_from(zip(p.ix[:,1],p.ix[:,0]))
clade=nx.dfs_tree(G,clade_top_tax_id)
if(not clade.nodes()):
clade.add_node(clade_top_tax_id)
return(clade.has_node(check_tax_id))
ncbi = NCBITaxa()
def subsample_taxids(taxids,rank='species'):
"""
For a given set of taxids leaves only one representative per selected rank
Eg. for a set of subspecies - leave only species.
"""
rank_dict={'superkingdom':0,'kingdom':1,'phylum':2,'class':3,'superorder':4,'order':5,'suborder':6,'infraorder':7,'parvorder':8,'superfamily':9,'family':10,'subfamily':11,'genus':12,'subgenus':13,'species':14,'subspecies':15}
tree = ncbi.get_topology(taxids,intermediate_nodes=True)
#We have now a phylogenetic tree with all annotations for our taxids.
subsampled_taxids=set()
#We are iterating through the taxids and for every we are determining only one representative from this group.
#These representatives will be the same for the taxids in one group - and hence subsampling will happen.
for t in taxids:
import csv
from os import listdir
from os.path import isfile, join
from collections import Counter
from collections import defaultdict
from ete2 import NCBITaxa
d = defaultdict(list)
taxids = []
mypath = raw_input("Enter path to csv files (i.e. /home/user/csv/files/): ")
allfiles = [f for f in listdir(mypath) if isfile(join(mypath, f))]
onlyfiles = [s for s in allfiles if '.csv' in s]
filecount = len(onlyfiles)
level = raw_input("1: phylum ----> 2: class ----> 3: order ----> 4: family ----> 5: genus ----> 6: species\nEnter rank number: ")
ncbi = NCBITaxa()
DEFAULT_TAXADB = os.path.join(os.environ.get('HOME', '/'), '.etetoolkit', 'taxa.sqlite')
DB_VERSION = 2
rank_dict = {"1": "phylum", "2": "class", "3": "order", "4": "family", "5": "genus", "6": "species"}
def is_taxadb_up_to_date(dbfile=DEFAULT_TAXADB):
"""
Check if a valid and up-to-date taxa.sqlite database exists
If dbfile is not specified, DEFAULT_TAXADB is assumed
:param dbfile:
:return:
"""
db = sqlite3.connect(dbfile)
try:
r = db.execute('SELECT version FROM stats;')
from ete2 import NCBITaxa
from ete2 import Tree, TreeStyle, AttrFace
ncbi = NCBITaxa()
input = [l.rstrip("\n") for l in open("db/example_input", "r")]
taxid = ncbi.get_name_translator(input)
tree = ncbi.get_topology(taxid.values())
#print tree.get_ascii(attributes=["sci_name", "rank", "taxid"])
# custom layout: adds "rank" on top of branches, and sci_name as tip names
def my_layout(node):
if getattr(node, "rank", None):
rank_face = AttrFace("rank", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
ts = TreeStyle()
ts.layout_fn = my_layout
ts.show_leaf_name = False
tree.render("tree.pdf", tree_style=ts)
import numpy as np
import pandas as pd
import cPickle as pickle
from ete2 import NCBITaxa
from pprint import pprint
import os.path
from Bio.Seq import Seq
from Bio.Alphabet import IUPAC
sys.path.append('/Volumes/MDBD/Dropbox/work/MYSOFT/ALIGNMENT_TOOLS/')
# Entrez.email = "*****@*****.**"
from hist_ss import get_core_lendiff
rank_dict={'superkingdom':0,'kingdom':1,'phylum':2,'class':3,'superorder':4,'order':5,'suborder':6,'infraorder':7,'parvorder':8,'superfamily':9,'family':10,'subfamily':11,'genus':12,'subgenus':13,'species':14,'subspecies':15}
ncbi = NCBITaxa()
def check_hist_length(seq,hist_type,hist_var=None,dev_percent=10):
"""
This simple check compares the length of sequence provided
to a range of curated sequences in histone DB +- dev_percent %.
"""
if(os.path.isfile('int_data/cur_length.csv')):
cur_df=pd.read_csv('int_data/cur_length.csv')
else:
hist_df=pd.read_csv('inp_data/seqs.csv') #Histone types info
fasta_dict=pickle.load( open( "int_data/fasta_dict.p", "rb" )) #Sequences
#construct df with length
cur_df=hist_df[(hist_df['curated']==True)]
cur_df['length']=cur_df['gi'].map(lambda x: len(fasta_dict[str(x)].seq))
# print cur_df.groupby(['hist_type','hist_var']).agg([np.max,np.min])
def taxo_seq_architecture(seqreclist=[],
outfile='taxo_arch.svg',
taxids=[],
annotation='',
title='',
width=2000):
"""
Visualize sequence architecture together with a taxonomy tree
seqreclist - contains a list of seqres.
each seqrec should have a list of features in biobython SeqFeature format.
features of type "domain" will be plotted as boxes
features of type "xxxx" will be plotted as ...
taxids - list of taxids in the same order as seqs in msa, if now provided will assume that seqrecs
are in genbank format and attempt to get taxids from there.
"""
aa = [
'A', 'R', 'N', 'D', 'C', 'Q', 'E', 'G', 'H', 'I', 'L', 'K', 'M', 'F',
'P', 'S', 'T', 'W', 'Y', 'V', 'B', 'Z', 'X', '.', '-'
]
def get_color(str):
colorlist = [
'red', 'green', 'yellow', 'lightblue', 'cyan', 'magenta', 'orange',
'pink', 'lightgreen'
]
return colorlist[hash(str) % 9]
if len(taxids) == 0:
taxids = map(get_taxid_from_gbrec, seqreclist)
ncbi = NCBITaxa()
taxids = map(int, taxids)
t = ncbi.get_topology(taxids, intermediate_nodes=False)
# a=t.add_child(name='annotation')
# a.add_feature('sci_name','annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if (node.rank in ['order', 'class', 'phylum', 'kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name == 'annotation':
#here we are adding faces and we need to play with seqmotif face
seq = str(seqreclist[taxids.index(int(node.name))].seq)
motifs = [] #[[0,len(seq), "seq", 10, 10, None, None, None]]
for f in seqreclist[taxids.index(int(node.name))].features:
if f.type == 'domain':
motifs.append([
f.location.start, f.location.end, "[]", None, 10,
"blue",
get_color(f.qualifiers['name']),
"arial|8|black|%s" % f.qualifiers['name']
])
if f.type == 'motif':
#It turns out that we need to solve overlap problem here, here it is solved only in case of one overlap
s = f.location.start
e = f.location.end
flag = True
overlappedm = []
for m in motifs:
if m[2] == 'seq' and m[0] < e and m[
1] > s: #we have an overlap, four cases, preceding motife always is on top
flag = False
overlappedm.append(m)
if not flag: #we have to solve multiple overlap problem
#let's do it by scanning
sflag = False
eflag = False
for x in range(s, e + 1):
if not sflag: #check if we can start
overlap = False
for m in overlappedm:
if x >= m[0] and x < m[1]:
overlap = True
if not overlap:
ts = x
sflag = True
#check if is time to end
if sflag and not eflag:
overlap = False
for m in overlappedm:
if x == m[0]:
overlap = True
if overlap or x == e:
te = x
eflag = True
if sflag and eflag:
motifs.append([
ts, te, "seq", 10, 10, "black",
get_color(f.qualifiers['name']), None
])
sflag = False
eflag = False
if flag:
motifs.append([
f.location.start, f.location.end, "seq", 10, 10,
"black",
get_color(f.qualifiers['name']), None
])
seqFace = SeqMotifFace(seq,
motifs,
scale_factor=1,
seq_format="[]")
seqFace.overlaping_motif_opacity = 1.0
# seqFace.fg=aafgcolors
# seqFace.bg=aabgcolors_gray
add_face_to_node(seqFace, node, 0, position="aligned")
# gi=taxid2gi[int(node.name)]
add_face_to_node(
TextFace(' ' + seqreclist[taxids.index(int(node.name))].id +
' '),
node,
column=1,
position="aligned")
# add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
#We currently disable annotation
if node.is_leaf() and node.name == 'annotation':
if (annotation):
s = annotation
# get_hist_ss_in_aln_as_string(msa_tr)
else:
s = ' ' * max(map(lambda x: len(x.seq), seqreclist))
# seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
# add_face_to_node(seqFace, node, 0, position="aligned")
# add_face_to_node(TextFace(' '+'SEQ_ID'),node,column=1, position = "aligned")
# add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render(outfile, w=width, dpi=300, tree_style=ts)
def taxo_msa(outfile='taxo_msa.svg',
taxids=[],
annotation='',
msa=[],
title='',
width=2000):
"""
Visualize MSA together with a taxonomy tree
taxids - list of taxids in the same order as seqs in msa
"""
# taxid2gi={f_df.loc[f_df.gi==int(gi),'taxid'].values[0]:gi for gi in list(f_df['gi'])}
# gi2variant={gi:f_df.loc[f_df.gi==int(gi),'hist_var'].values[0] for gi in list(f_df['gi'])}
# msa_dict={i.id:i.seq for i in msa_tr}
ncbi = NCBITaxa()
taxids = map(int, taxids)
t = ncbi.get_topology(taxids, intermediate_nodes=False)
a = t.add_child(name='annotation')
a.add_feature('sci_name', 'annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if (node.rank in ['order', 'class', 'phylum', 'kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name == 'annotation':
s = str(msa[taxids.index(int(node.name))].seq)
seqFace = SeqMotifFace(
s, [[0, len(s), "seq", 10, 10, None, None, None]],
scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
# gi=taxid2gi[int(node.name)]
add_face_to_node(TextFace(' ' +
msa[taxids.index(int(node.name))].id),
node,
column=1,
position="aligned")
# add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
if node.is_leaf() and node.name == 'annotation':
if (annotation):
s = annotation
# get_hist_ss_in_aln_as_string(msa_tr)
else:
s = ' ' * len(msa[0].seq)
seqFace = SeqMotifFace(
s, [[0, len(s), "seq", 10, 10, None, None, None]],
scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
add_face_to_node(TextFace(' ' + 'SEQ_ID'),
node,
column=1,
position="aligned")
# add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render(outfile, w=width, dpi=300, tree_style=ts)
def run(args):
# add lineage profiles/stats
import re
from ete2 import PhyloTree, NCBITaxa
# dump tree by default
if not args.tree and not args.info and not args.descendants:
args.tree = True
ncbi = NCBITaxa()
all_taxids = {}
all_names = set()
queries = []
if not args.search:
log.error('Search terms should be provided (i.e. --search) ')
sys.exit(-1)
for n in args.search:
queries.append(n)
try:
all_taxids[int(n)] = None
except ValueError:
all_names.add(n.strip())
# translate names
name2tax = ncbi.get_name_translator(all_names)
all_taxids.update([(v, None) for v in name2tax.values()])
not_found_names = all_names - set(name2tax.keys())
if args.fuzzy and not_found_names:
log.warn("%s unknown names", len(not_found_names))
for name in not_found_names:
# enable extension loading
tax, realname, sim = ncbi.get_fuzzy_name_translation(name, args.fuzzy)
if tax:
all_taxids[tax] = None
name2tax[name] = tax
name2realname[name] = realname
name2score[name] = "Fuzzy:%0.2f" %sim
if not_found_names:
log.warn("[%s] could not be translated into taxids!" %','.join(not_found_names))
if args.tree:
if len(all_taxids) == 1:
target_taxid = all_taxids.keys()[0]
log.info("Dumping NCBI descendants tree for %s" %(target_taxid))
t = ncbi.get_descendant_taxa(target_taxid, collapse_subspecies=args.collapse_subspecies, rank_limit=args.rank_limit, return_tree=True)
else:
log.info("Dumping NCBI taxonomy of %d taxa..." %(len(all_taxids)))
t = ncbi.get_topology(all_taxids.keys(),
intermediate_nodes=args.full_lineage,
rank_limit=args.rank_limit,
collapse_subspecies=args.collapse_subspecies)
id2name = ncbi.get_taxid_translator([n.name for n in t.traverse()])
for n in t.traverse():
n.add_features(taxid=n.name)
n.add_features(sci_name=str(id2name.get(int(n.name), "?")))
n.name = "%s - %s" %(id2name.get(int(n.name), n.name), n.name)
lineage = ncbi.get_lineage(n.taxid)
n.add_features(named_lineage = '|'.join(ncbi.translate_to_names(lineage)))
dump(t, features=["taxid", "name", "rank", "bgcolor", "sci_name",
"collapse_subspecies", "named_lineage"])
elif args.descendants:
log.info("Dumping NCBI taxonomy of %d taxa..." %(len(all_taxids)))
print '# ' + '\t'.join(["Taxid", "Sci.Name", "Rank", "descendant_taxids", "descendant_names"])
translator = ncbi.get_taxid_translator(all_taxids)
ranks = ncbi.get_rank(all_taxids)
for taxid in all_taxids:
descendants = ncbi.get_descendant_taxa(taxid, collapse_subspecies=args.collapse_subspecies, rank_limit=args.rank_limit)
print '\t'.join([str(taxid), translator.get(taxid, taxid), ranks.get(taxid, ''),
'|'.join(map(str, descendants)),
'|'.join(map(str, ncbi.translate_to_names(descendants)))])
elif args.info:
print '# ' + '\t'.join(["Taxid", "Sci.Name", "Rank", "Named Lineage", "Taxid Lineage"])
translator = ncbi.get_taxid_translator(all_taxids)
ranks = ncbi.get_rank(all_taxids)
for taxid, name in translator.iteritems():
lineage = ncbi.get_lineage(taxid)
named_lineage = ','.join(ncbi.translate_to_names(lineage))
lineage_string = ','.join(map(str, lineage))
print '\t'.join([str(taxid), name, ranks.get(taxid, ''), named_lineage, lineage_string])
def taxo_seq_architecture(seqreclist=[],outfile='taxo_arch.svg',taxids=[],annotation='',title='',width=2000):
"""
Visualize sequence architecture together with a taxonomy tree
seqreclist - contains a list of seqres.
each seqrec should have a list of features in biobython SeqFeature format.
features of type "domain" will be plotted as boxes
features of type "xxxx" will be plotted as ...
taxids - list of taxids in the same order as seqs in msa, if now provided will assume that seqrecs
are in genbank format and attempt to get taxids from there.
"""
aa=['A','R','N','D','C','Q','E','G','H','I','L','K','M','F','P','S','T','W','Y','V','B','Z','X','.','-']
def get_color(str):
colorlist=['red','green','yellow','lightblue','cyan','magenta','orange','pink','lightgreen']
return colorlist[hash(str)%9]
if len(taxids)==0:
taxids=map(get_taxid_from_gbrec,seqreclist)
ncbi = NCBITaxa()
taxids=map(int,taxids)
t = ncbi.get_topology(taxids,intermediate_nodes=False)
# a=t.add_child(name='annotation')
# a.add_feature('sci_name','annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if(node.rank in ['order','class','phylum','kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name=='annotation':
#here we are adding faces and we need to play with seqmotif face
seq=str(seqreclist[taxids.index(int(node.name))].seq)
motifs=[]#[[0,len(seq), "seq", 10, 10, None, None, None]]
for f in seqreclist[taxids.index(int(node.name))].features:
if f.type=='domain':
motifs.append([f.location.start,f.location.end,"[]",None,10,"blue", get_color(f.qualifiers['name']), "arial|8|black|%s"%f.qualifiers['name']])
if f.type=='motif':
#It turns out that we need to solve overlap problem here, here it is solved only in case of one overlap
s=f.location.start
e=f.location.end
flag=True
overlappedm=[]
for m in motifs:
if m[2]=='seq' and m[0]<e and m[1]>s: #we have an overlap, four cases, preceding motife always is on top
flag=False
overlappedm.append(m)
if not flag: #we have to solve multiple overlap problem
#let's do it by scanning
sflag=False
eflag=False
for x in range(s,e+1):
if not sflag: #check if we can start
overlap=False
for m in overlappedm:
if x>=m[0] and x<m[1]:
overlap=True
if not overlap:
ts=x
sflag=True
#check if is time to end
if sflag and not eflag:
overlap=False
for m in overlappedm:
if x==m[0]:
overlap=True
if overlap or x==e:
te=x
eflag=True
if sflag and eflag:
motifs.append([ts,te,"seq",10,10,"black",get_color(f.qualifiers['name']),None])
sflag=False
eflag=False
if flag:
motifs.append([f.location.start,f.location.end,"seq",10,10,"black",get_color(f.qualifiers['name']),None])
seqFace = SeqMotifFace(seq,motifs,scale_factor=1,seq_format="[]")
seqFace.overlaping_motif_opacity = 1.0
# seqFace.fg=aafgcolors
# seqFace.bg=aabgcolors_gray
add_face_to_node(seqFace, node, 0, position="aligned")
# gi=taxid2gi[int(node.name)]
add_face_to_node(TextFace(' '+seqreclist[taxids.index(int(node.name))].id+' '),node,column=1, position = "aligned")
# add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
#We currently disable annotation
if node.is_leaf() and node.name=='annotation':
if(annotation):
s=annotation
# get_hist_ss_in_aln_as_string(msa_tr)
else:
s=' '*max(map(lambda x: len(x.seq),seqreclist))
# seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
# add_face_to_node(seqFace, node, 0, position="aligned")
# add_face_to_node(TextFace(' '+'SEQ_ID'),node,column=1, position = "aligned")
# add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
# add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render(outfile, w=width, dpi=300, tree_style=ts)
def main():
args = parser.parse_args()
input_fp = args.input_fp
tdt_out_fp = args.tdt_out_fp
h2gt_out_fp = args.h2gt_out_fp
rank_headers = args.rank_headers
ranks = args.ranks
ncbi = NCBITaxa()
# input_fp = './R1_trimmed_CAT_rare_genefamilies_cpm_ko.tsv'
h2gt = read_humann2_genetable_generator(open(input_fp))
rank_headers = rank_headers.split(',')
ranks = ranks.split(',')
tax_dict = {}
if tdt_out_fp:
tdt_out_f = open(tdt_out_fp, 'w')
if h2gt_out_fp:
h2gt_out_f = open(h2gt_out_fp, 'w')
first_h = True
first_t = True
for gene, header, line, tax in h2gt:
lineage = list(rank_headers)
if tax and tax not in tax_dict:
best_id = None
family, genus, species = clean_humann2_taxon(tax)
best_id = get_best_ncbi_id(family, genus, species, ncbi)
if best_id is not None:
lineage = get_taxon_path(best_id,
ncbi,
ranks=ranks,
rank_headers=rank_headers)
tax_dict[tax] = lineage
if tax:
lineage = tax_dict[tax]
if tdt_out_fp:
if first_t:
first_t = False
tdt_out_f.write('Gene Family\t{0}\t{1}\n'.format(
'\t'.join(header), '\t'.join(ranks)))
elif tax:
tdt_out_f.write('{0}\t{1}\t{2}\n'.format(
gene, '\t'.join(line), '\t'.join(lineage)))
if h2gt_out_fp:
if first_h:
h2gt_out_f.write('# Gene Family\t{0}\n'.format(
'\t'.join(header)))
first_h = False
if tax:
if lineage == rank_headers:
h2gt_out_f.write('{0}|{1}\t{2}\n'.format(
gene, 'unknown', '\t'.join(line)))
else:
h2gt_out_f.write('{0}|{1}\t{2}\n'.format(
gene, '.'.join(lineage), '\t'.join(line)))
else:
h2gt_out_f.write('{0}\t{1}\n'.format(gene, '\t'.join(line)))
if tdt_out_fp:
tdt_out_f.close()
if h2gt_out_f:
h2gt_out_f.close()
def get_name(taxid):
ncbi = NCBITaxa()
names = ncbi.get_taxid_translator([taxid])
return names[taxid]
from pandas import DataFrame
from Bio import SeqIO
from pandas import Index
from ete2 import NCBITaxa
data_path = "/home/moritz/people/MoreData/genomes/img_od1s"
img_fasta = "/home/moritz/people/MoreData/raw_imgs/od1s.fasta"
img_xls = "/home/moritz/people/MoreData/raw_imgs/od1s.xls"
name = "parcu_from_img_"
taxDb = NCBITaxa()
contigs = DataFrame.from_csv(img_xls, sep="\t", header=0, index_col=0)
manual_taxo = taxDb.get_name_translator(['Candidatus Parcubacteria'
]).values()[0][0]
metadata = {
name + str(g): {
'IMG_ID': g,
'name': name + str(g),
'species_taxid': manual_taxo,
'long_name': contigs.loc[contigs['Genome ID'] == g]['Genome'].iloc[0]
}
for g in set(contigs['Genome ID'])
}
seq_dict = {k: [] for k in metadata}
with open(img_fasta, "r") as file:
for i, c in enumerate(SeqIO.parse(file, "fasta")):
seq_dict[name + str(contigs.iloc[i]['Genome ID'])] += [c]
val = [color[2] for color in hsv]
ind = np.lexsort((val, sat, hue))
sorted_colors = [colors_[i] for i in ind]
colors_final = []
for i, (name, color) in enumerate(sorted_colors):
colors_final.append(color)
import random
random.shuffle(colors_final)
colors_mapping = {}
#set up NCBI database
from ete2 import NCBITaxa
ncbi = NCBITaxa('/dfs/scratch0/manans/.etetoolkit/taxa.sqlite')
# read .nemb file
EMBEDDING_FILE = 'emb/n2v-avg.nemb'
histograms = []
species_ids = []
with open(EMBEDDING_FILE, 'r') as tf:
for line in tf:
ls = line.split(' ')
species_ids.append(ls[0])
v = []
for n in ls[1:]:
v.append(float(n))
histograms.append(v)
from ete2 import NCBITaxa
from ete2 import Tree, TreeStyle, AttrFace
ncbi = NCBITaxa()
input = [l.rstrip("\n") for l in open("db/example_input", "r")]
taxid = ncbi.get_name_translator(input)
tree = ncbi.get_topology(taxid.values())
#print tree.get_ascii(attributes=["sci_name", "rank", "taxid"])
# custom layout: adds "rank" on top of branches, and sci_name as tip names
def my_layout(node):
if getattr(node, "rank", None):
rank_face = AttrFace("rank", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
ts = TreeStyle()
ts.layout_fn = my_layout
ts.show_leaf_name = False
tree.render("tree.pdf", tree_style=ts)