def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
# Grid
self.gridButton = guitools.BetterPushButton('Grid')
self.gridButton.setCheckable(True)
self.gridButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
# Crosshair
self.crosshairButton = guitools.BetterPushButton('Crosshair')
self.crosshairButton.setCheckable(True)
self.crosshairButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
# liveview
self.liveviewButton = guitools.BetterPushButton('LIVEVIEW')
self.liveviewButton.setStyleSheet("font-size:20px")
self.liveviewButton.setCheckable(True)
self.liveviewButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
self.liveviewButton.setEnabled(True)
# Add elements to GridLayout
self.viewCtrlLayout = QtWidgets.QGridLayout()
self.setLayout(self.viewCtrlLayout)
self.viewCtrlLayout.addWidget(self.liveviewButton, 0, 0, 1, 2)
self.viewCtrlLayout.addWidget(self.gridButton, 1, 0)
self.viewCtrlLayout.addWidget(self.crosshairButton, 1, 1)
# Connect signals
self.gridButton.toggled.connect(self.sigGridToggled)
self.crosshairButton.toggled.connect(self.sigCrosshairToggled)
self.liveviewButton.toggled.connect(self.sigLiveviewToggled)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
self.scanInLiveviewWar = QtWidgets.QMessageBox()
self.scanInLiveviewWar.setInformativeText(
"You need to be in liveview to scan")
self.digModWarning = QtWidgets.QMessageBox()
self.digModWarning.setInformativeText(
"You need to be in digital laser modulation and external "
"frame-trigger acquisition mode")
self.saveScanBtn = guitools.BetterPushButton('Save Scan')
self.loadScanBtn = guitools.BetterPushButton('Load Scan')
self.seqTimePar = QtWidgets.QLineEdit('10') # ms
self.nrFramesPar = QtWidgets.QLabel()
self.scanDuration = 0
self.scanDurationLabel = QtWidgets.QLabel(str(self.scanDuration))
self.scanDims = []
self.scanPar = {'seqTime': self.seqTimePar}
self.pxParameters = {}
self.pxParValues = {}
self.scanRadio = QtWidgets.QRadioButton('Scan')
self.scanRadio.setChecked(True)
self.contLaserPulsesRadio = QtWidgets.QRadioButton(
'Cont. Laser Pulses')
self.scanButton = guitools.BetterPushButton('Run Scan')
self.continuousCheck = QtWidgets.QCheckBox('Repeat')
self.graph = GraphFrame()
self.graph.setEnabled(False)
self.graph.setFixedHeight(128)
self.grid = QtWidgets.QGridLayout()
self.setLayout(self.grid)
# Connect signals
self.saveScanBtn.clicked.connect(self.sigSaveScanClicked)
self.loadScanBtn.clicked.connect(self.sigLoadScanClicked)
self.scanButton.clicked.connect(self.sigRunScanClicked)
self.seqTimePar.textChanged.connect(self.sigSeqTimeParChanged)
self.contLaserPulsesRadio.toggled.connect(
self.sigContLaserPulsesToggled)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
detectorTitle = QtWidgets.QLabel(
'<h2><strong>Detector settings</strong></h2>')
detectorTitle.setTextFormat(QtCore.Qt.RichText)
self.ROI = guitools.VispyROIVisual(rect_color='yellow',
handle_color='orange')
self.stack = QtWidgets.QStackedWidget()
self.trees = {}
self.detectorListBox = QtWidgets.QHBoxLayout()
self.detectorListLabel = QtWidgets.QLabel('Current detector:')
self.detectorList = QtWidgets.QComboBox()
self.nextDetectorButton = guitools.BetterPushButton('Next')
self.nextDetectorButton.hide()
self.detectorListBox.addWidget(self.detectorListLabel)
self.detectorListBox.addWidget(self.detectorList, 1)
self.detectorListBox.addWidget(self.nextDetectorButton)
# Add elements to GridLayout
self.layout = QtWidgets.QVBoxLayout()
self.setLayout(self.layout)
self.layout.addWidget(detectorTitle)
self.layout.addWidget(self.stack)
self.layout.addLayout(self.detectorListBox)
# Connect signals
self.ROI.sigROIChanged.connect(self.sigROIChanged)
self.detectorList.currentIndexChanged.connect(
lambda index: self.sigDetectorChanged.emit(
self.detectorList.itemData(index)))
self.nextDetectorButton.clicked.connect(self.sigNextDetectorClicked)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
self.angleEdit = QtWidgets.QLineEdit('30')
self.alignmentCheck = QtWidgets.QCheckBox('Show Alignment Tool')
self.alignmentLineMakerButton = guitools.BetterPushButton(
'Alignment Line')
pen = pg.mkPen(color=(255, 255, 0),
width=0.5,
style=QtCore.Qt.SolidLine,
antialias=True)
self.alignmentLine = guitools.VispyLineVisual(movable=True)
# Add items to GridLayout
alignmentLayout = QtWidgets.QGridLayout()
self.setLayout(alignmentLayout)
alignmentLayout.addWidget(QtWidgets.QLabel('Line Angle'), 0, 0)
alignmentLayout.addWidget(self.angleEdit, 0, 1)
alignmentLayout.addWidget(self.alignmentLineMakerButton, 1, 0)
alignmentLayout.addWidget(self.alignmentCheck, 1, 1)
# Connect signals
self.alignmentLineMakerButton.clicked.connect(
self.sigAlignmentLineMakeClicked)
self.alignmentCheck.toggled.connect(self.sigAlignmentCheckToggled)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Viewbox
self.cwidget = pg.GraphicsLayoutWidget()
self.vb = self.cwidget.addViewBox(row=1, col=1)
self.vb.setMouseMode(pg.ViewBox.RectMode)
self.img = pg.ImageItem(axisOrder='row-major')
self.img.translate(-0.5, -0.5)
self.vb.addItem(self.img)
self.vb.setAspectLocked(True)
self.hist = pg.HistogramLUTItem(image=self.img)
self.hist.vb.setLimits(yMin=0, yMax=66000)
self.hist.gradient.loadPreset('greyclip')
for tick in self.hist.gradient.ticks:
tick.hide()
self.cwidget.addItem(self.hist, row=1, col=2)
self.roiButton = guitools.BetterPushButton('Show ROI')
self.roiButton.setCheckable(True)
self.runButton = QtWidgets.QCheckBox('Run')
self.ROI = guitools.VispyROIVisual(rect_color='yellow',
handle_color='orange')
# Add elements to GridLayout
grid = QtWidgets.QGridLayout()
self.setLayout(grid)
grid.addWidget(self.cwidget, 0, 0, 1, 6)
grid.addWidget(self.roiButton, 1, 0, 1, 1)
grid.addWidget(self.runButton, 1, 1, 1, 1)
# Connect signals
self.roiButton.toggled.connect(self.sigROIToggled)
self.runButton.clicked.connect(self.sigRunClicked)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical Elements
self.ulensesButton = guitools.BetterPushButton('uLenses')
self.ulensesCheck = QtWidgets.QCheckBox('Show uLenses')
self.xEdit = QtWidgets.QLineEdit('0')
self.yEdit = QtWidgets.QLineEdit('0')
self.pxEdit = QtWidgets.QLineEdit('157.5')
self.upEdit = QtWidgets.QLineEdit('1182')
self.ulensesPlot = guitools.VispyScatterVisual(color='red', symbol='x')
# Add elements to GridLayout
ulensesLayout = QtWidgets.QGridLayout()
self.setLayout(ulensesLayout)
ulensesLayout.addWidget(QtWidgets.QLabel('Pixel Size'), 0, 0)
ulensesLayout.addWidget(self.pxEdit, 0, 1)
ulensesLayout.addWidget(QtWidgets.QLabel('Periodicity'), 1, 0)
ulensesLayout.addWidget(self.upEdit, 1, 1)
ulensesLayout.addWidget(QtWidgets.QLabel('X offset'), 2, 0)
ulensesLayout.addWidget(self.xEdit, 2, 1)
ulensesLayout.addWidget(QtWidgets.QLabel('Y offset'), 3, 0)
ulensesLayout.addWidget(self.yEdit, 3, 1)
ulensesLayout.addWidget(self.ulensesButton, 4, 0)
ulensesLayout.addWidget(self.ulensesCheck, 4, 1)
# Connect signals
self.ulensesButton.clicked.connect(self.sigULensesClicked)
self.ulensesCheck.toggled.connect(self.sigUShowLensesChanged)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
guitools.addNapariGrayclipColormap()
self.napariViewer = napari.Viewer(show=False)
self.imgLayers = {}
self.levelsButton = guitools.BetterPushButton('Update Levels', minMinWidth=180)
self.levelsButton.setMinimumHeight(40)
self.toolboxLayout = QtWidgets.QHBoxLayout()
self.toolboxLayout.addSpacerItem(
QtWidgets.QSpacerItem(
40, 20, QtWidgets.QSizePolicy.Expanding, QtWidgets.QSizePolicy.Minimum
)
)
self.toolboxLayout.addWidget(self.levelsButton)
self.viewCtrlLayout = QtWidgets.QVBoxLayout()
self.viewCtrlLayout.addWidget(self.napariViewer.window._qt_window)
self.viewCtrlLayout.addLayout(self.toolboxLayout)
self.setLayout(self.viewCtrlLayout)
self.grid = guitools.VispyGridVisual(color='yellow')
self.grid.hide()
self.addItem(self.grid)
self.crosshair = guitools.VispyCrosshairVisual(color='yellow')
self.crosshair.hide()
self.addItem(self.crosshair)
# Connect signals
self.levelsButton.clicked.connect(self.sigUpdateLevelsClicked)
def addPositioner(self, positionerName, axes):
for i in range(len(axes)):
axis = axes[i]
parNameSuffix = self._getParNameSuffix(positionerName, axis)
label = f'{positionerName} -- {axis}' if positionerName != axis else positionerName
self.pars['Label' + parNameSuffix] = QtWidgets.QLabel(
f'<strong>{label}</strong>')
self.pars['Label' + parNameSuffix].setTextFormat(
QtCore.Qt.RichText)
self.pars['Position' + parNameSuffix] = QtWidgets.QLabel(
f'<strong>{0:.2f} µm</strong>')
self.pars['Position' + parNameSuffix].setTextFormat(
QtCore.Qt.RichText)
self.pars['UpButton' +
parNameSuffix] = guitools.BetterPushButton('+')
self.pars['DownButton' +
parNameSuffix] = guitools.BetterPushButton('-')
self.pars['StepEdit' + parNameSuffix] = QtWidgets.QLineEdit('0.05')
self.pars['StepUnit' + parNameSuffix] = QtWidgets.QLabel(' µm')
self.grid.addWidget(self.pars['Label' + parNameSuffix],
self.numPositioners, 0)
self.grid.addWidget(self.pars['Position' + parNameSuffix],
self.numPositioners, 1)
self.grid.addWidget(self.pars['UpButton' + parNameSuffix],
self.numPositioners, 2)
self.grid.addWidget(self.pars['DownButton' + parNameSuffix],
self.numPositioners, 3)
self.grid.addWidget(QtWidgets.QLabel('Step'), self.numPositioners,
4)
self.grid.addWidget(self.pars['StepEdit' + parNameSuffix],
self.numPositioners, 5)
self.grid.addWidget(self.pars['StepUnit' + parNameSuffix],
self.numPositioners, 6)
self.numPositioners += 1
# Connect signals
self.pars['UpButton' + parNameSuffix].clicked.connect(
lambda: self.sigStepUpClicked.emit(positionerName, axis))
self.pars['DownButton' + parNameSuffix].clicked.connect(
lambda: self.sigStepDownClicked.emit(positionerName, axis))
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
self.roiButton = guitools.BetterPushButton('Show ROI')
self.roiButton.setCheckable(True)
self.resetButton = guitools.BetterPushButton('Reset graph')
self.ROI = guitools.VispyROIVisual(rect_color='yellow',
handle_color='orange')
self.graph = guitools.SumpixelsGraph()
self.resetButton.clicked.connect(self.graph.resetData)
# Add items to GridLayout
grid = QtWidgets.QGridLayout()
self.setLayout(grid)
grid.addWidget(self.graph, 0, 0, 1, 6)
grid.addWidget(self.roiButton, 1, 0, 1, 1)
grid.addWidget(self.resetButton, 1, 1, 1, 1)
# grid.setRowMinimumHeight(0, 300)
# Connect signals
self.roiButton.toggled.connect(self.sigShowROIToggled)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
self.powers = {}
self.grid = QtWidgets.QGridLayout()
self.setLayout(self.grid)
title = QtWidgets.QLabel('<h3>Digital modulation<h3>')
title.setTextFormat(QtCore.Qt.RichText)
title.setAlignment(QtCore.Qt.AlignCenter)
title.setStyleSheet("font-size:12px")
title.setFixedHeight(20)
self.grid.addWidget(title, 0, 0, 1, -1)
self.DigitalControlButton = guitools.BetterPushButton('Enable')
self.DigitalControlButton.setCheckable(True)
self.grid.addWidget(self.DigitalControlButton, 2, 0, 1, -1)
# Connect signals
self.DigitalControlButton.toggled.connect(self.sigDigitalModToggled)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
self.roiButton = guitools.BetterPushButton('Show ROI')
self.roiButton.setCheckable(True)
self.XButton = QtWidgets.QRadioButton('X dimension')
self.YButton = QtWidgets.QRadioButton('Y dimension')
self.ROI = guitools.VispyROIVisual(rect_color='yellow',
handle_color='orange')
self.graph = guitools.ProjectionGraph()
# Add elements to GridLayout
grid = QtWidgets.QGridLayout()
self.setLayout(grid)
grid.addWidget(self.graph, 0, 0, 1, 6)
grid.addWidget(self.roiButton, 1, 0, 1, 1)
grid.addWidget(self.XButton, 1, 1, 1, 1)
grid.addWidget(self.YButton, 1, 2, 1, 1)
# Connect signals
self.roiButton.toggled.connect(self.sigShowROIToggled)
self.XButton.clicked.connect(lambda: self.sigAxisChanged.emit(0))
self.YButton.clicked.connect(lambda: self.sigAxisChanged.emit(1))
def __init__(self, name, units, wavelength, valueRange, tickInterval, singleStep,
initialPower, *args, **kwargs):
super().__init__(*args, **kwargs)
isBinary = valueRange is None
# Graphical elements
self.setFrameStyle(QtWidgets.QFrame.Panel | QtWidgets.QFrame.Raised)
self.setMinimumWidth(180)
self.name = QtWidgets.QLabel(f'<h3>{name}<h3>')
self.name.setTextFormat(QtCore.Qt.RichText)
self.name.setAlignment(QtCore.Qt.AlignCenter)
color = guitools.colorutils.wavelengthToHex(wavelength)
self.name.setStyleSheet(f'font-size:16px; border-bottom: 4px solid {color}')
self.name.setFixedHeight(40)
self.setPointLabel = QtWidgets.QLabel('Setpoint')
self.setPointEdit = QtWidgets.QLineEdit(str(initialPower))
self.setPointEdit.setFixedWidth(50)
self.setPointEdit.setAlignment(QtCore.Qt.AlignRight)
self.powerLabel = QtWidgets.QLabel('Power')
self.powerIndicator = QtWidgets.QLabel(str(initialPower))
self.powerIndicator.setFixedWidth(50)
self.powerIndicator.setAlignment(QtCore.Qt.AlignRight)
self.minpower = QtWidgets.QLabel()
self.minpower.setAlignment(QtCore.Qt.AlignCenter)
self.maxpower = QtWidgets.QLabel()
self.maxpower.setAlignment(QtCore.Qt.AlignCenter)
self.slider = QtWidgets.QSlider(QtCore.Qt.Vertical, self)
self.slider.setFocusPolicy(QtCore.Qt.NoFocus)
if not isBinary:
valueRangeMin, valueRangeMax = valueRange
self.minpower.setText(str(valueRangeMin))
self.maxpower.setText(str(valueRangeMax))
self.slider.setMinimum(valueRangeMin)
self.slider.setMaximum(valueRangeMax)
self.slider.setTickInterval(tickInterval)
self.slider.setSingleStep(singleStep)
self.slider.setValue(0)
powerFrame = QtWidgets.QFrame(self)
self.powerGrid = QtWidgets.QGridLayout()
powerFrame.setFrameStyle(QtWidgets.QFrame.Panel | QtWidgets.QFrame.Plain)
powerFrame.setLayout(self.powerGrid)
self.powerGrid.addWidget(self.setPointLabel, 1, 0, 1, 2)
self.powerGrid.addWidget(self.setPointEdit, 2, 0)
self.powerGrid.addWidget(QtWidgets.QLabel(units), 2, 1)
self.powerGrid.addWidget(self.powerLabel, 3, 0, 1, 2)
self.powerGrid.addWidget(self.powerIndicator, 4, 0)
self.powerGrid.addWidget(QtWidgets.QLabel(units), 4, 1)
self.powerGrid.addWidget(self.maxpower, 0, 3)
self.powerGrid.addWidget(self.slider, 1, 3, 8, 1)
self.powerGrid.addWidget(self.minpower, 9, 3)
self.enableButton = guitools.BetterPushButton('ON')
self.enableButton.setCheckable(True)
# Add elements to GridLayout
self.grid = QtWidgets.QGridLayout()
self.setLayout(self.grid)
self.grid.addWidget(self.name, 0, 0, 1, 2)
self.grid.addWidget(powerFrame, 1, 0, 1, 2)
if isBinary:
sizePolicy = powerFrame.sizePolicy()
sizePolicy.setRetainSizeWhenHidden(True)
powerFrame.setSizePolicy(sizePolicy)
powerFrame.hide()
self.grid.addWidget(self.enableButton, 8, 0, 1, 2)
# Connect signals
self.enableButton.toggled.connect(self.sigEnableChanged)
self.slider.valueChanged[int].connect(
lambda value: self.sigValueChanged.emit(float(value))
)
self.setPointEdit.returnPressed.connect(
lambda: self.sigValueChanged.emit(self.getValue())
)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
self.slmFrame = pg.GraphicsLayoutWidget()
self.vb = self.slmFrame.addViewBox(row=1, col=1)
self.img = pg.ImageItem()
self.img.setImage(np.zeros((792, 600)),
autoLevels=True,
autoDownsample=True,
autoRange=True)
self.vb.addItem(self.img)
self.vb.setAspectLocked(True)
self.slmParameterTree = ParameterTree()
self.generalparams = [
{
'name':
'General parameters',
'type':
'group',
'children': [
{
'name': 'Radius',
'type': 'float',
'value': 100,
'limits': (0, 600),
'step': 1,
'suffix': 'px'
},
{
'name': 'Sigma',
'type': 'float',
'value': 35,
'limits': (1, 599),
'step': 0.1,
'suffix': 'px'
},
# {'name': 'Angle', 'type': 'float', 'value': 0.15, 'limits': (0, 0.3), 'step': 0.01, 'suffix': 'rad'},
# {'name': 'Wavelength', 'type': 'float', 'value': 775, 'limits': (0, 1200), 'step': 1, 'suffix': 'nm'},
# {'name': 'Helix rotation', 'type': 'bool', 'value': True},
]
},
{
'name': 'Apply',
'type': 'action'
}
]
self.slmParameterTree.setStyleSheet("""
QTreeView::item, QAbstractSpinBox, QComboBox {
padding-top: 0;
padding-bottom: 0;
border: none;
}
QComboBox QAbstractItemView {
min-width: 128px;
}
""")
self.slmParameterTree.p = pg.parametertree.Parameter.create(
name='params', type='group', children=self.generalparams)
self.slmParameterTree.setParameters(self.slmParameterTree.p,
showTop=False)
self.slmParameterTree._writable = True
self.aberParameterTree = pg.parametertree.ParameterTree()
aberlim = 2
self.aberparams = [{
'name':
'Donut',
'type':
'group',
'children': [{
'name': 'Tilt factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Tip factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Defocus factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Spherical factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Vertical coma factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Horizontal coma factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Vertical astigmatism factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Oblique astigmatism factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}]
}, {
'name':
'Tophat',
'type':
'group',
'children': [{
'name': 'Tilt factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Tip factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Defocus factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Spherical factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Vertical coma factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Horizontal coma factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Vertical astigmatism factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}, {
'name': 'Oblique astigmatism factor',
'type': 'float',
'value': 0,
'limits': (-aberlim, aberlim),
'step': 0.01
}]
}, {
'name': 'Apply',
'type': 'action'
}]
self.aberParameterTree.setStyleSheet("""
QTreeView::item, QAbstractSpinBox, QComboBox {
padding-top: 0;
padding-bottom: 0;
border: none;
}
QComboBox QAbstractItemView {
min-width: 128px;
}
""")
self.aberParameterTree.p = pg.parametertree.Parameter.create(
name='params', type='group', children=self.aberparams)
self.aberParameterTree.setParameters(self.aberParameterTree.p,
showTop=False)
self.aberParameterTree._writable = True
self.paramtreeDockArea = pg.dockarea.DockArea()
pmtreeDock = pg.dockarea.Dock('Phase mask parameters', size=(1, 1))
pmtreeDock.addWidget(self.slmParameterTree)
self.paramtreeDockArea.addDock(pmtreeDock)
abertreeDock = pg.dockarea.Dock('Aberration correction parameters',
size=(1, 1))
abertreeDock.addWidget(self.aberParameterTree)
self.paramtreeDockArea.addDock(abertreeDock, 'above', pmtreeDock)
# Control panel with most buttons
self.controlPanel = QtWidgets.QFrame()
self.controlPanel.choiceInterfaceLayout = QtWidgets.QGridLayout()
self.controlPanel.choiceInterface = QtWidgets.QWidget()
self.controlPanel.choiceInterface.setLayout(
self.controlPanel.choiceInterfaceLayout)
# Choose which mask to modify
self.controlPanel.maskComboBox = QtWidgets.QComboBox()
self.controlPanel.maskComboBox.addItem("Donut (left)")
self.controlPanel.maskComboBox.addItem("Top hat (right)")
self.controlPanel.choiceInterfaceLayout.addWidget(
QtWidgets.QLabel('Select mask:'), 0, 0)
self.controlPanel.choiceInterfaceLayout.addWidget(
self.controlPanel.maskComboBox, 0, 1)
# Choose which objective is in use
self.controlPanel.objlensComboBox = QtWidgets.QComboBox()
self.controlPanel.objlensComboBox.addItem("No objective")
self.controlPanel.objlensComboBox.addItem("Oil")
self.controlPanel.objlensComboBox.addItem("Glycerol")
self.controlPanel.choiceInterfaceLayout.addWidget(
QtWidgets.QLabel('Select objective:'), 1, 0)
self.controlPanel.choiceInterfaceLayout.addWidget(
self.controlPanel.objlensComboBox, 1, 1)
# Phase mask moving buttons
self.controlPanel.arrowButtons = []
self.controlPanel.upButton = guitools.BetterPushButton('Up (YZ)')
self.controlPanel.arrowButtons.append(self.controlPanel.upButton)
self.controlPanel.downButton = guitools.BetterPushButton('Down (YZ)')
self.controlPanel.arrowButtons.append(self.controlPanel.downButton)
self.controlPanel.leftButton = guitools.BetterPushButton('Left (XZ)')
self.controlPanel.arrowButtons.append(self.controlPanel.leftButton)
self.controlPanel.rightButton = guitools.BetterPushButton('Right (XZ)')
self.controlPanel.arrowButtons.append(self.controlPanel.rightButton)
for button in self.controlPanel.arrowButtons:
button.setCheckable(False)
button.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
button.setFixedSize(self.controlPanel.upButton.sizeHint())
# Interface to change the amount of displacement induced by the arrows
self.controlPanel.incrementInterface = QtWidgets.QWidget()
self.controlPanel.incrementInterfaceLayout = QtWidgets.QVBoxLayout()
self.controlPanel.incrementInterface.setLayout(
self.controlPanel.incrementInterfaceLayout)
self.controlPanel.incrementlabel = QtWidgets.QLabel("Step (px)")
self.controlPanel.incrementSpinBox = QtWidgets.QSpinBox()
self.controlPanel.incrementSpinBox.setRange(1, 50)
self.controlPanel.incrementSpinBox.setValue(1)
self.controlPanel.incrementInterfaceLayout.addWidget(
self.controlPanel.incrementlabel)
self.controlPanel.incrementInterfaceLayout.addWidget(
self.controlPanel.incrementSpinBox)
# Interface to change the rotation angle of phase pattern
self.controlPanel.rotationInterface = QtWidgets.QWidget()
self.controlPanel.rotationInterfaceLayout = QtWidgets.QVBoxLayout()
self.controlPanel.rotationInterface.setLayout(
self.controlPanel.rotationInterfaceLayout)
self.controlPanel.rotationLabel = QtWidgets.QLabel(
'Pattern angle (rad)')
self.controlPanel.rotationEdit = QtWidgets.QLineEdit('0')
self.controlPanel.rotationInterfaceLayout.addWidget(
self.controlPanel.rotationLabel)
self.controlPanel.rotationInterfaceLayout.addWidget(
self.controlPanel.rotationEdit)
# Buttons for saving, loading, and controlling the various phase patterns
self.controlPanel.saveButton = guitools.BetterPushButton("Save")
self.controlPanel.loadButton = guitools.BetterPushButton("Load")
self.controlPanel.donutButton = guitools.BetterPushButton("Donut")
self.controlPanel.tophatButton = guitools.BetterPushButton("Tophat")
self.controlPanel.blackButton = guitools.BetterPushButton(
"Black frame")
self.controlPanel.gaussianButton = guitools.BetterPushButton(
"Gaussian")
self.controlPanel.halfButton = guitools.BetterPushButton(
"Half pattern")
self.controlPanel.quadrantButton = guitools.BetterPushButton(
"Quad pattern")
self.controlPanel.hexButton = guitools.BetterPushButton("Hex pattern")
self.controlPanel.splitbullButton = guitools.BetterPushButton(
"Split pattern")
# Defining layout
self.controlPanel.arrowsFrame = QtWidgets.QFrame()
self.controlPanel.arrowsLayout = QtWidgets.QGridLayout()
self.controlPanel.arrowsFrame.setLayout(self.controlPanel.arrowsLayout)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.upButton, 0,
1)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.leftButton,
1, 0)
self.controlPanel.arrowsLayout.addWidget(
self.controlPanel.incrementInterface, 1, 1)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.rightButton,
1, 2)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.downButton,
2, 1)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.loadButton,
0, 3)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.saveButton,
1, 3)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.donutButton,
3, 0)
self.controlPanel.arrowsLayout.addWidget(
self.controlPanel.tophatButton, 3, 1)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.blackButton,
4, 0)
self.controlPanel.arrowsLayout.addWidget(
self.controlPanel.gaussianButton, 4, 1)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.halfButton,
5, 0)
self.controlPanel.arrowsLayout.addWidget(
self.controlPanel.quadrantButton, 5, 1)
self.controlPanel.arrowsLayout.addWidget(self.controlPanel.hexButton,
6, 0)
self.controlPanel.arrowsLayout.addWidget(
self.controlPanel.splitbullButton, 6, 1)
self.controlPanel.arrowsLayout.addWidget(
self.controlPanel.rotationInterface, 5, 2, 2, 1)
# Definition of the box layout:
self.controlPanel.boxLayout = QtWidgets.QVBoxLayout()
self.controlPanel.setLayout(self.controlPanel.boxLayout)
self.controlPanel.boxLayout.addWidget(
self.controlPanel.choiceInterface)
self.controlPanel.boxLayout.addWidget(self.controlPanel.arrowsFrame)
self.grid = QtWidgets.QGridLayout()
self.setLayout(self.grid)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Focus lock
self.kpEdit = QtWidgets.QLineEdit('5')
self.kpLabel = QtWidgets.QLabel('kp')
self.kiEdit = QtWidgets.QLineEdit('0.1')
self.kiLabel = QtWidgets.QLabel('ki')
self.lockButton = guitools.BetterPushButton('Lock')
self.lockButton.setCheckable(True)
self.lockButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
self.zStackBox = QtWidgets.QCheckBox('Z-stack')
self.twoFociBox = QtWidgets.QCheckBox('Two foci')
self.zStepFromEdit = QtWidgets.QLineEdit('40')
self.zStepFromLabel = QtWidgets.QLabel('Min step (nm)')
self.zStepToEdit = QtWidgets.QLineEdit('100')
self.zStepToLabel = QtWidgets.QLabel('Max step (nm)')
# self.focusDataBox = QtWidgets.QCheckBox('Save data') # Connect to exportData
self.camDialogButton = guitools.BetterPushButton('Camera Dialog')
# Piezo absolute positioning
self.positionLabel = QtWidgets.QLabel(
'Position (µm)'
) # Potentially disregard this and only use in the positioning widget?
self.positionEdit = QtWidgets.QLineEdit('50')
self.positionSetButton = guitools.BetterPushButton('Set')
# Focus lock calibration
self.calibFromLabel = QtWidgets.QLabel('From (µm)')
self.calibFromEdit = QtWidgets.QLineEdit('49')
self.calibToLabel = QtWidgets.QLabel('To (µm)')
self.calibToEdit = QtWidgets.QLineEdit('51')
self.focusCalibButton = guitools.BetterPushButton('Calib')
self.focusCalibButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
self.calibCurveButton = guitools.BetterPushButton('See calib')
self.calibrationDisplay = QtWidgets.QLineEdit(
'Previous calib: none'
) # Edit this from the controller with calibration values
self.calibrationDisplay.setReadOnly(True)
# CREATE CALIBRATION CURVE WINDOW AND FOCUS CALIBRATION GRAPH SOMEHOW
# Focus lock graph
self.focusLockGraph = pg.GraphicsLayoutWidget()
self.focusLockGraph.setAntialiasing(True)
self.focusPlot = self.focusLockGraph.addPlot(row=1, col=0)
self.focusPlot.setLabels(bottom=('Time', 's'),
left=('Laser position', 'px'))
self.focusPlot.showGrid(x=True, y=True)
self.focusPlotCurve = self.focusPlot.plot(
pen='y'
) # update this (self.focusPlotCurve.setData(X,Y)) with update(focusSignal) function
# Webcam graph
self.webcamGraph = pg.GraphicsLayoutWidget()
self.camImg = pg.ImageItem(border='w')
self.camImg.setImage(np.zeros((100, 100)))
self.vb = self.webcamGraph.addViewBox(invertY=True, invertX=False)
self.vb.setAspectLocked(True)
self.vb.addItem(self.camImg)
# PROCESS DATA THREAD - ADD SOMEWHERE ELSE, NOT HERE, AS IT HAS NO GRAPHICAL ELEMENTS!
# GUI layout below
grid = QtWidgets.QGridLayout()
self.setLayout(grid)
grid.addWidget(self.focusLockGraph, 0, 0, 1, 9)
grid.addWidget(self.webcamGraph, 0, 9, 4, 1)
grid.addWidget(self.focusCalibButton, 1, 2, 2, 1)
grid.addWidget(self.calibrationDisplay, 3, 0, 1, 2)
grid.addWidget(self.kpLabel, 1, 3)
grid.addWidget(self.kpEdit, 1, 4)
grid.addWidget(self.kiLabel, 2, 3)
grid.addWidget(self.kiEdit, 2, 4)
grid.addWidget(self.lockButton, 1, 5, 2, 1)
grid.addWidget(self.zStackBox, 4, 2)
grid.addWidget(self.twoFociBox, 4, 6)
grid.addWidget(self.zStepFromLabel, 3, 4)
grid.addWidget(self.zStepFromEdit, 4, 4)
grid.addWidget(self.zStepToLabel, 3, 5)
grid.addWidget(self.zStepToEdit, 4, 5)
# grid.addWidget(self.focusDataBox, 4, 0, 1, 2)
grid.addWidget(self.calibFromLabel, 1, 0)
grid.addWidget(self.calibFromEdit, 1, 1)
grid.addWidget(self.calibToLabel, 2, 0)
grid.addWidget(self.calibToEdit, 2, 1)
grid.addWidget(self.calibCurveButton, 3, 2)
grid.addWidget(self.positionLabel, 1, 6)
grid.addWidget(self.positionEdit, 1, 7)
grid.addWidget(self.positionSetButton, 2, 6, 1, 2)
grid.addWidget(self.camDialogButton, 3, 6, 1, 2)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
self.scanInLiveviewWar = QtWidgets.QMessageBox()
self.scanInLiveviewWar.setInformativeText(
"You need to be in liveview to scan")
self.digModWarning = QtWidgets.QMessageBox()
self.digModWarning.setInformativeText(
"You need to be in digital laser modulation and external "
"frame-trigger acquisition mode")
self.saveScanBtn = guitools.BetterPushButton('Save Scan')
self.loadScanBtn = guitools.BetterPushButton('Load Scan')
self.seqTimePar = QtWidgets.QLineEdit('10') # ms
self.nrFramesPar = QtWidgets.QLabel()
self.scanDuration = 0
self.scanDurationLabel = QtWidgets.QLabel(str(self.scanDuration))
self.scanDims = []
self.scanPar = {'seqTime': self.seqTimePar}
self.pxParameters = {}
self.pxParValues = {}
self.scanRadio = QtWidgets.QRadioButton('Scan')
self.scanRadio.setChecked(True)
self.contLaserPulsesRadio = QtWidgets.QRadioButton(
'Cont. Laser Pulses')
self.scanButton = guitools.BetterPushButton('Run Scan')
self.continuousCheck = QtWidgets.QCheckBox('Repeat')
self.graph = GraphFrame()
self.graph.setEnabled(False)
self.graph.setFixedHeight(128)
self.scrollContainer = QtWidgets.QGridLayout()
self.scrollContainer.setContentsMargins(0, 0, 0, 0)
self.setLayout(self.scrollContainer)
self.grid = QtWidgets.QGridLayout()
self.gridContainer = QtWidgets.QWidget()
self.gridContainer.setLayout(self.grid)
self.scrollArea = QtWidgets.QScrollArea()
self.scrollArea.setFrameShape(QtWidgets.QFrame.NoFrame)
self.scrollArea.setHorizontalScrollBarPolicy(
QtCore.Qt.ScrollBarAsNeeded)
self.scrollArea.setVerticalScrollBarPolicy(QtCore.Qt.ScrollBarAsNeeded)
self.scrollArea.setWidget(self.gridContainer)
self.scrollArea.setWidgetResizable(True)
self.scrollContainer.addWidget(self.scrollArea)
self.gridContainer.installEventFilter(self)
# Connect signals
self.saveScanBtn.clicked.connect(self.sigSaveScanClicked)
self.loadScanBtn.clicked.connect(self.sigLoadScanClicked)
self.scanButton.clicked.connect(self.sigRunScanClicked)
self.seqTimePar.textChanged.connect(self.sigSeqTimeParChanged)
self.contLaserPulsesRadio.toggled.connect(
self.sigContLaserPulsesToggled)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
self.showCheck = QtWidgets.QCheckBox('Show FFT')
self.showCheck.setCheckable(True)
self.posCheck = guitools.BetterPushButton('Period (pix)')
self.posCheck.setCheckable(True)
self.linePos = QtWidgets.QLineEdit('4')
self.lineRate = QtWidgets.QLineEdit('0')
self.labelRate = QtWidgets.QLabel('Update rate')
# Vertical and horizontal lines
self.vline = pg.InfiniteLine()
self.hline = pg.InfiniteLine()
self.rvline = pg.InfiniteLine()
self.lvline = pg.InfiniteLine()
self.uhline = pg.InfiniteLine()
self.dhline = pg.InfiniteLine()
# Viewbox
self.cwidget = pg.GraphicsLayoutWidget()
self.vb = self.cwidget.addViewBox(row=1, col=1)
self.vb.setMouseMode(pg.ViewBox.RectMode)
self.img = guitools.OptimizedImageItem(axisOrder='row-major')
self.img.translate(-0.5, -0.5)
self.vb.addItem(self.img)
self.vb.setAspectLocked(True)
self.hist = pg.HistogramLUTItem(image=self.img)
self.hist.vb.setLimits(yMin=0, yMax=66000)
self.hist.gradient.loadPreset('greyclip')
for tick in self.hist.gradient.ticks:
tick.hide()
self.cwidget.addItem(self.hist, row=1, col=2)
# Add lines to viewbox
self.vb.addItem(self.vline)
self.vb.addItem(self.hline)
self.vb.addItem(self.lvline)
self.vb.addItem(self.rvline)
self.vb.addItem(self.uhline)
self.vb.addItem(self.dhline)
# Add elements to GridLayout
grid = QtWidgets.QGridLayout()
self.setLayout(grid)
grid.addWidget(self.cwidget, 0, 0, 1, 6)
grid.addWidget(self.showCheck, 1, 0, 1, 1)
grid.addWidget(self.posCheck, 2, 0, 1, 1)
grid.addWidget(self.linePos, 2, 1, 1, 1)
grid.addWidget(self.labelRate, 2, 2, 1, 1)
grid.addWidget(self.lineRate, 2, 3, 1, 1)
# grid.setRowMinimumHeight(0, 300)
# Connect signals
self.showCheck.toggled.connect(self.sigShowToggled)
self.posCheck.toggled.connect(self.sigPosToggled)
self.linePos.textChanged.connect(
lambda: self.sigPosChanged.emit(self.getPos()))
self.lineRate.textChanged.connect(
lambda: self.sigUpdateRateChanged.emit(self.getUpdateRate()))
self.vb.sigResized.connect(self.sigResized)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# Graphical elements
recTitle = QtWidgets.QLabel('<h2><strong>Recording settings</strong></h2>')
recTitle.setTextFormat(QtCore.Qt.RichText)
# Detector list
self.detectorList = QtWidgets.QComboBox()
# Folder and filename fields
baseOutputFolder = self._options.recording.outputFolder
if self._options.recording.includeDateInOutputFolder:
self.initialDir = os.path.join(baseOutputFolder, time.strftime('%Y-%m-%d'))
else:
self.initialDir = baseOutputFolder
self.folderEdit = QtWidgets.QLineEdit(self.initialDir)
self.openFolderButton = guitools.BetterPushButton('Open')
self.specifyfile = QtWidgets.QCheckBox('Specify file name')
self.filenameEdit = QtWidgets.QLineEdit('Current time')
# Snap and recording buttons
self.snapTIFFButton = guitools.BetterPushButton('Snap')
self.snapTIFFButton.setStyleSheet("font-size:16px")
self.snapTIFFButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
self.recButton = guitools.BetterPushButton('REC')
self.recButton.setStyleSheet("font-size:16px")
self.recButton.setCheckable(True)
self.recButton.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
# Number of frames and measurement timing
modeTitle = QtWidgets.QLabel('<strong>Recording mode</strong>')
modeTitle.setTextFormat(QtCore.Qt.RichText)
self.specifyFrames = QtWidgets.QRadioButton('Number of frames')
self.specifyTime = QtWidgets.QRadioButton('Time (s)')
self.recScanOnceBtn = QtWidgets.QRadioButton('Scan once')
self.recScanLapseBtn = QtWidgets.QRadioButton('Time-lapse scan')
self.currentLapse = QtWidgets.QLabel('0 / ')
self.timeLapseEdit = QtWidgets.QLineEdit('5')
self.freqLabel = QtWidgets.QLabel('Freq [s]')
self.freqEdit = QtWidgets.QLineEdit('0')
self.stepSizeLabel = QtWidgets.QLabel('Step size [um]')
self.stepSizeEdit = QtWidgets.QLineEdit('0.05')
self.untilSTOPbtn = QtWidgets.QRadioButton('Run until STOP')
self.timeToRec = QtWidgets.QLineEdit('1')
self.currentTime = QtWidgets.QLabel('0 / ')
self.currentTime.setAlignment((QtCore.Qt.AlignRight |
QtCore.Qt.AlignVCenter))
self.currentFrame = QtWidgets.QLabel('0 /')
self.currentFrame.setAlignment((QtCore.Qt.AlignRight |
QtCore.Qt.AlignVCenter))
self.numExpositionsEdit = QtWidgets.QLineEdit('100')
self.tRemaining = QtWidgets.QLabel()
self.tRemaining.setAlignment((QtCore.Qt.AlignCenter |
QtCore.Qt.AlignVCenter))
self.saveModeLabel = QtWidgets.QLabel('<strong>Save mode:</strong>')
self.saveModeList = QtWidgets.QComboBox()
self.saveModeList.addItems(['Save on disk',
'Save in memory for reconstruction',
'Save on disk and keep in memory'])
# Add items to GridLayout
buttonWidget = QtWidgets.QWidget()
buttonGrid = QtWidgets.QGridLayout()
buttonWidget.setLayout(buttonGrid)
buttonGrid.addWidget(self.snapTIFFButton, 0, 0)
buttonWidget.setSizePolicy(QtWidgets.QSizePolicy.Preferred,
QtWidgets.QSizePolicy.Expanding)
buttonGrid.addWidget(self.recButton, 0, 2)
recGrid = QtWidgets.QGridLayout()
self.setLayout(recGrid)
recGrid.addWidget(recTitle, 0, 0, 1, 3)
recGrid.addWidget(QtWidgets.QLabel('Detector to capture'), 1, 0)
recGrid.addWidget(self.detectorList, 1, 1, 1, 4)
recGrid.addWidget(QtWidgets.QLabel('Folder'), 2, 0)
recGrid.addWidget(self.folderEdit, 2, 1, 1, 3)
recGrid.addWidget(self.openFolderButton, 2, 4)
recGrid.addWidget(self.filenameEdit, 3, 1, 1, 3)
recGrid.addWidget(self.specifyfile, 3, 0)
recGrid.addWidget(modeTitle, 4, 0)
recGrid.addWidget(self.specifyFrames, 5, 0, 1, 5)
recGrid.addWidget(self.currentFrame, 5, 1)
recGrid.addWidget(self.numExpositionsEdit, 5, 2)
recGrid.addWidget(self.specifyTime, 6, 0, 1, 5)
recGrid.addWidget(self.currentTime, 6, 1)
recGrid.addWidget(self.timeToRec, 6, 2)
recGrid.addWidget(self.tRemaining, 6, 3, 1, 2)
recGrid.addWidget(self.recScanOnceBtn, 7, 0, 1, 5)
recGrid.addWidget(self.recScanLapseBtn, 8, 0, 1, 5)
recGrid.addWidget(self.currentLapse, 8, 1)
recGrid.addWidget(self.timeLapseEdit, 8, 2)
recGrid.addWidget(self.freqLabel, 8, 3)
recGrid.addWidget(self.freqEdit, 8, 4)
recGrid.addWidget(self.untilSTOPbtn, 10, 0, 1, -1)
recGrid.addWidget(self.saveModeLabel, 12, 0)
recGrid.addWidget(self.saveModeList, 12, 1, 1, -1)
recGrid.addWidget(buttonWidget, 13, 0, 1, -1)
# Initial condition of fields and checkboxes.
self.writable = True
self.readyToRecord = False
self.filenameEdit.setEnabled(False)
self.untilSTOPbtn.setChecked(True)
# Connect signals
self.detectorList.currentIndexChanged.connect(self.sigDetectorChanged)
self.openFolderButton.clicked.connect(self.sigOpenRecFolderClicked)
self.specifyfile.toggled.connect(self.sigSpecFileToggled)
self.snapTIFFButton.clicked.connect(self.sigSnapRequested)
self.recButton.toggled.connect(self.sigRecToggled)
self.specifyFrames.clicked.connect(self.sigSpecFramesPicked)
self.specifyTime.clicked.connect(self.sigSpecTimePicked)
self.recScanOnceBtn.clicked.connect(self.sigScanOncePicked)
self.recScanLapseBtn.clicked.connect(self.sigScanLapsePicked)
self.untilSTOPbtn.clicked.connect(self.sigUntilStopPicked)