def run(type):
if type not in [COHORT_ANALYSIS, SINGLE_TUMOR_ANALYSIS]:
abort(400)
'''
if g.demo and current_user.max_analysis != -1 and proj_manager.get_projects_count(g.conn, g.user_id) >= current_user.max_analysis:
flash("""The online version is for demo only and there is a limit for the number of simultaneous analysis a user can manage.
You must remove finished analysis before running new ones.
Please download the pipeline and install in your system to avoid these limitations.""", "error")
return redirect(url_for("download"))
'''
cb = ConfigBuilder(current_app.wok.conf_builder)
cb.add_value("workspace", "default")
conf = cb.get_conf()
results_path = os.path.join(conf["results_path"], current_user.nick)
cb.add_value("results_path", results_path)
temp_path = os.path.join(conf["temp_path"], current_user.nick)
cb.add_value("temp_path", temp_path)
if not current_user.is_anonymous():
cb.add_value("website.user_id", current_user.nick)
if type == COHORT_ANALYSIS:
project_id = "cohort-example"
mutations_path = get_examples_path(conf, "meduloblastoma_cohort_tier1.muts")
elif type == SINGLE_TUMOR_ANALYSIS:
project_id = "single-tumor-example"
mutations_path = get_examples_path(conf, "pat4_crc.muts")
cb.add_value("variants_only", True)
cb.add_value("skip_oncodrivefm", True)
cb.add_value("skip_oncodriveclust", True)
i = 0
base_id = project_id
while current_app.wok.exists_case(current_user, project_id):
i += 1
project_id = "{}-{}".format(base_id, i)
cb.add_value("project.id", project_id)
conf = cb.get_conf()
project_path = get_project_path(conf, project_id)
if not os.path.exists(project_path):
os.makedirs(project_path)
project_temp_path = get_temp_path(conf, project_id)
if not os.path.exists(project_temp_path):
os.makedirs(project_temp_path)
assembly = "hg19"
cb, flow_uri = project_analysis(
mutations_path,
assembly=assembly,
conf_builder=cb)
properties = dict(
analysis_type=type,
path=project_path,
temp_path=project_temp_path,
data_file=mutations_path)
current_app.logger.info("[{}] Starting example {} ...".format(
current_user.nick, project_id))
case = current_app.wok.create_case(current_user, project_id, cb, flow_uri,
properties=properties, start=True)
current_app.logger.info("[{}] Example {} started on case {}...".format(
current_user.nick, project_id, case.engine_name))
return redirect(url_for("cases.index", highlight=case.id))
def run(type):
if type not in [COHORT_ANALYSIS, SINGLE_TUMOR_ANALYSIS]:
abort(400)
if request.method == "GET":
form = dict(
ofm_genes_threshold=ONCODRIVEFM_GENES_THRESHOLD,
ofm_pathways_threshold=ONCODRIVEFM_PATHWAYS_THRESHOLD,
oclust_genes_threshold=ONCODRIVECLUST_MUTATIONS_THRESHOLD)
return render_template("analysis.html", type=type, form=form)
mutations_file = request.files['mutations_file']
file_name = os.path.basename(mutations_file.filename)
project_id = request.form['project_name']
if len(project_id) == 0:
project_id = os.path.splitext(file_name)[0]
project_id = normalize_id(project_id)
i = 0
base_id = project_id
while current_app.wok.exists_case(current_user, project_id):
i += 1
project_id = "{}-{}".format(base_id, i)
'''
if current_app.wok.exists_case(current_user, project_id):
flash("An analysis with this name already exists. Please give it a different name or remove the previous one before starting again.", "error")
return render_template("analysis.html", type=type, form=request.form)
'''
'''
if g.demo and current_user.max_analysis != -1 and proj_manager.get_projects_count(g.conn, g.user_id) >= current_user.max_analysis:
flash("""The online version is for demo only and there is a limit for the number of simultaneous analysis a user can manage.
You must remove finished analysis before running new ones.
Please download the pipeline and install in your system to avoid these limitations.""", "error")
return redirect(url_for("download"))
if not current_user.validated:
flash("""You can not run an analysis with your data until you are completely registered.
Please check your email and follow the instructions to validate this account.""", "error")
flash("Meanwhile you can play with the included examples.")
return redirect(url_for("examples"))
'''
cb = ConfigBuilder(current_app.wok.conf_builder)
cb.add_value("workspace", "default")
cb.add_value("project.id", project_id)
#case_name = "-".join([current_user.nick, project_id])
#cb.add_value("wok.instance.name", case_name)
conf = cb.get_conf()
results_path = os.path.join(conf["results_path"], current_user.nick)
cb.add_value("results_path", results_path)
temp_path = os.path.join(conf["temp_path"], current_user.nick)
cb.add_value("temp_path", temp_path)
conf = cb.get_conf()
project_path = get_project_path(conf, project_id)
if not os.path.exists(project_path):
os.makedirs(project_path)
project_temp_path = get_temp_path(conf, project_id)
if not os.path.exists(project_temp_path):
os.makedirs(project_temp_path)
if not current_user.is_anonymous():
cb.add_value("website.user_id", current_user.nick)
# FIXME ? type == SINGLE_TUMOR_ANALYSIS
if request.form.get("variants_only") == "1":
cb.add_value("variants_only", True)
cb.add_value("skip_oncodrivefm", True)
cb.add_value("skip_oncodriveclust", True)
try:
threshold = request.form["ofm_genes_threshold"]
if re.match(r"^[1-9]\d*%?$", threshold):
cb.add_value(ONCODRIVEFM_GENES_THRESHOLD_KEY, threshold)
except:
if type == COHORT_ANALYSIS:
current_app.logger.warn("Undefined form input: {}".format("ofm_genes_threshold"))
try:
threshold = request.form["ofm_pathways_threshold"]
if re.match(r"^[1-9]\d*%?$", threshold):
cb.add_value(ONCODRIVEFM_PATHWAYS_THRESHOLD_KEY, threshold)
except:
if type == COHORT_ANALYSIS:
current_app.logger.warn("Undefined form input: {}".format("ofm_pathways_threshold"))
try:
threshold = int(request.form["oclust_genes_threshold"])
if threshold >= 1:
cb.add_value(ONCODRIVECLUST_GENES_THRESHOLD_KEY, threshold)
except:
if type == COHORT_ANALYSIS:
current_app.logger.warn("Undefined form input: {}".format("oclust_genes_threshold"))
genes_filter_enabled = request.form.get('genes_filter_enabled') == "1"
cb.add_value(ONCODRIVEFM_FILTER_ENABLED_KEY, genes_filter_enabled)
cb.add_value(ONCODRIVECLUST_FILTER_ENABLED_KEY, genes_filter_enabled)
if genes_filter_enabled:
try:
genes_filter_file = request.files['genes_filter_file']
genes_filter_file_path = os.path.join(project_temp_path, "genes-filter.txt")
genes_filter_file.save(genes_filter_file_path)
if os.path.getsize(genes_filter_file_path) != 0:
cb.add_value(ONCODRIVEFM_GENES_FILTER_KEY, genes_filter_file_path)
cb.add_value(ONCODRIVECLUST_GENES_FILTER_KEY, genes_filter_file_path)
except:
current_app.logger.exception("Error retrieving genes filter from form")
mutations_path = os.path.join(project_temp_path, file_name)
try:
mutations_file.save(mutations_path)
except:
current_app.logger.exception("Error while saving mutations file {} into {}".format(mutations_file.filename, mutations_path))
flash("""There were some problem with the input file for mutations.
Please check that a file has been loaded before submitting a new analysis.
This error has been already submitted to the application administrators
who will take care of it as soon as possible.""")
return render_template("analysis.html", type=type, form=request.form)
assembly = request.form.get("assembly", "hg19").lower()
cb, flow_uri = project_analysis(
mutations_path,
assembly=assembly,
conf_builder=cb)
properties = dict(
analysis_type=type,
path=project_path,
temp_path=project_temp_path,
data_file=mutations_path)
current_app.logger.info("[{}] Starting analysis {} ...".format(
current_user.nick, project_id))
case = current_app.wok.create_case(current_user, project_id, cb, flow_uri,
properties=properties, start=True)
current_app.logger.info("[{}] Analysis {} started on case {}...".format(
current_user.nick, project_id, case.engine_name))
return redirect(url_for("cases.index", highlight=case.id))
def make_project(log, conf, project, base_path=None):
project_id = project["id"]
project_path = get_project_path(conf, project_id)
if not os.path.exists(project_path):
os.makedirs(project_path)
temp_path = get_temp_path(conf, project_id)
if not os.path.exists(temp_path):
os.makedirs(temp_path)
projdb_path = os.path.join(project_path, "project.db")
if "annotations" in project:
annotations = project["annotations"]
if not isinstance(annotations, dict):
log.warn("Overriding project annotations field with an empty dictionary")
project["annotations"] = annotations = {}
else:
project["annotations"] = annotations = {}
for key in project.keys():
if key not in ["id", "assembly", "files", "annotations", "oncodriveclust", "oncodrivefm"]:
value = project[key]
del project[key]
annotations[key] = value
project["path"] = project_path
project["temp_path"] = temp_path
project["db"] = projdb_path
if "assembly" not in project:
project["assembly"] = "hg19"
files = project["files"]
# make absolute paths if necessary
if base_path is not None:
for i, file in enumerate(files):
if not os.path.isabs(file):
files[i] = os.path.join(base_path, file)
missing_files = []
# copy variants files into project path
for i, file in enumerate(files):
file_base_name = os.path.basename(file)
if not os.path.dirname(file).startswith(project_path):
dst_name = "{0:02d}-{1}".format(i, file_base_name)
dst_path = os.path.join(project_path, dst_name)
log.info(" {0} --> {1}".format(file_base_name, dst_name))
if os.path.exists(file):
shutil.copy(file, dst_path)
files[i] = dst_path
else:
missing_files += [file]
else:
log.info(" {0}".format(file_base_name))
if len(missing_files) > 0:
raise Exception("Project {0} references some missing files:\n{1}".format(project_id, "\n".join(missing_files)))
# save project.conf
projres = ProjectResults(project)
projres.save_def()