def create_sequencing_process(user, pools):
seq_process = SequencingProcess.create(
user, pools, 'New sequencing run %s' % datetime.now(),
'Run experiment %s' % datetime.now(), Equipment(18), 151, 151,
User('*****@*****.**'),
contacts=[User('*****@*****.**'), User('*****@*****.**')])
return seq_process
def post(self):
email = self.get_argument('email')
op = self.get_argument('operation')
if op == 'grant':
User(email).grant_access()
elif op == 'revoke':
User(email).revoke_access()
else:
raise HTTPError(400, 'Operation %s not recognized')
self.finish()
def post(self):
pool_id = self.get_argument('pool')
run_name = self.get_argument('run_name')
sequencer_id = self.get_argument('sequencer')
fwd_cycles = int(self.get_argument('fwd_cycles'))
rev_cycles = int(self.get_argument('rev_cycles'))
assay = self.get_argument('assay')
pi = self.get_argument('principal_investigator')
c0 = self.get_argument('contact_0')
c1 = self.get_argument('contact_1')
c2 = self.get_argument('contact_2')
process = SequencingProcess.create(
self.current_user, PoolComposition(pool_id), run_name,
Equipment(sequencer_id), fwd_cycles, rev_cycles, assay,
User(pi), User(c0), User(c1), User(c2))
self.write({'process': process.id})
def test_attributes(self):
s = Study(1)
self.assertEqual(
s.title, 'Identification of the Microbiomes '
'for Cannabis Soils')
self.assertEqual(s.creator, User('*****@*****.**'))
self.assertEqual(s.num_samples, 27)
def test_plate_handler_patch_request(self):
tester = Plate(21)
user = User('*****@*****.**')
# Incorrect path parameter
regex = 'Incorrect path parameter'
with self.assertRaisesRegex(HTTPError, regex):
plate_handler_patch_request(user, 21, 'replace', '/name/newname',
'NewName', None)
# Unknown attribute
regex = 'Attribute unknown not recognized'
with self.assertRaisesRegex(HTTPError, regex):
plate_handler_patch_request(user, 21, 'replace', '/unknown/',
'NewName', None)
# Unknown operation
regex = ('Operation add not supported. Current supported '
'operations: replace')
with self.assertRaisesRegex(HTTPError, regex):
plate_handler_patch_request(user, 21, 'add', '/name/', 'NewName',
None)
# Plate doesn't exist
regex = 'Plate 100 doesn\'t exist'
with self.assertRaisesRegex(HTTPError, regex):
plate_handler_patch_request(user, 100, 'replace', '/name/',
'NewName', None)
# Test success - Name
plate_handler_patch_request(user, 21, 'replace', '/name/', 'NewName',
None)
self.assertEqual(tester.external_id, 'NewName')
tester.external_id = 'Test plate 1'
def stress_tests(num_plates):
"""Creates num_plates plates and complete the amplicon/shotgun workflow
"""
samples = get_samples()
user = User('*****@*****.**')
stress_tests_amplicon_workflow(user, samples, num_plates=num_plates)
stress_tests_shotgun_workflow(user, samples, num_plates=num_plates)
def post(self):
pools = self.get_argument('pools')
run_name = self.get_argument('run_name')
experiment = self.get_argument('experiment')
sequencer_id = self.get_argument('sequencer')
fwd_cycles = int(self.get_argument('fwd_cycles'))
rev_cycles = int(self.get_argument('rev_cycles'))
pi = self.get_argument('principal_investigator')
contacts = self.get_argument('additional_contacts')
pools = [PoolComposition(x) for x in json_decode(pools)]
contacts = [User(x) for x in json_decode(contacts)]
process = SequencingProcess.create(self.current_user, pools,
run_name, experiment,
Equipment(sequencer_id), fwd_cycles,
rev_cycles, User(pi), contacts)
self.write({'process': process.id})
def get(self):
sequencers = []
for model, lanes in SequencingProcess.sequencer_lanes.items():
for sequencer in Equipment.list_equipment(model):
sequencer['lanes'] = lanes
sequencers.append(sequencer)
self.render('sequencing.html',
users=User.list_users(),
sequencers=sequencers)
def get_current_user(self):
"""Get the current connected user"""
username = self.get_secure_cookie("user")
if username is not None:
# strip off quotes added by get_secure_cookie and decode
# becuase it is stored as character varying in the DB
return User(username.strip(b"\"' ").decode())
else:
self.clear_cookie("user")
return None
def integration_tests():
"""Creates one amplicon and one shotgun workflow from plating to sequencing
"""
samples = get_samples()
user = User('*****@*****.**')
amplicon_seq_process = integration_tests_amplicon_workflow(user, samples)
obs = amplicon_seq_process.generate_sample_sheet()
res = re.match(EXP_AMPLICON_SAMPLE_SHEET, obs)
if res is None:
raise ValueError(
'Amplicon sample sheet does not match expected regex:\n%s' % obs)
def test_access_handler_post(self):
tester = User('*****@*****.**')
response = self.post('/auth/access/', {
'email': '*****@*****.**',
'operation': 'grant'
})
self.assertEqual(response.code, 200)
self.assertEqual(User.login('*****@*****.**', 'password'), tester)
response = self.post('/auth/access/', {
'email': '*****@*****.**',
'operation': 'revoke'
})
self.assertEqual(response.code, 200)
with self.assertRaises(LabmanLoginDisabledError):
User.login('*****@*****.**', 'password')
response = self.post('/auth/access/', {
'email': '*****@*****.**',
'operation': 'unknown'
})
self.assertEqual(response.code, 400)
def test_sample_plating_process_handler_patch_request(self):
user = User('*****@*****.**')
# Test operation not supported
regex = ('Operation add not supported. Current supported '
'operations: replace')
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'add', '/well/8/1/', '1.SKM8.640201', None)
# Test incorrect path parameter
regex = 'Incorrect path parameter'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/8/1/', '1.SKM8.640201', None)
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/content', '1.SKM8.640201',
None)
# Test attribute not found
regex = 'Attribute content not found'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/content/8/1/', '1.SKM8.640201', None)
# Test missing req_value
regex = 'A new value for the well should be provided'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/', None, None)
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/', '', None)
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/', ' ', None)
# Test success
obs = SampleComposition(85)
self.assertEqual(obs.sample_composition_type, 'blank')
self.assertIsNone(obs.sample_id)
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/', '1.SKM8.640201', None)
self.assertEqual(obs.sample_composition_type, 'experimental sample')
self.assertEqual(obs.sample_id, '1.SKM8.640201')
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/', 'blank', None)
self.assertEqual(obs.sample_composition_type, 'blank')
self.assertIsNone(obs.sample_id)
def stress_tests(num_plates_amplicon, num_plates_shotgun, user):
"""Creates num_plates plates and complete the amplicon/shotgun workflow
"""
samples = get_samples()
user = User(user)
if num_plates_amplicon:
stress_tests_amplicon_workflow(user,
samples,
num_plates=num_plates_amplicon)
if num_plates_shotgun:
stress_tests_shotgun_workflow(user,
samples,
num_plates=num_plates_shotgun)
def test_login(self):
exp = User('*****@*****.**')
obs = User.login('*****@*****.**', 'password')
self.assertEqual(obs, exp)
with self.assertRaises(LabmanUnknownIdError):
User.login('*****@*****.**', 'password')
with self.assertRaises(LabmanLoginError):
User.login('*****@*****.**', 'wrongpassword')
def test_get_previously_plated_wells(self):
tester = Plate(21)
three_plates_list = [Plate(27), Plate(30), Plate(33)]
exp = {Well(3073): three_plates_list, Well(3079): three_plates_list,
Well(3085): three_plates_list, Well(3091): three_plates_list,
Well(3097): three_plates_list, Well(3103): three_plates_list,
Well(3121): three_plates_list, Well(3127): three_plates_list,
Well(3133): three_plates_list, Well(3139): three_plates_list,
Well(3145): three_plates_list, Well(3151): three_plates_list,
Well(3169): three_plates_list, Well(3175): three_plates_list,
Well(3181): three_plates_list, Well(3187): three_plates_list,
Well(3193): three_plates_list, Well(3199): three_plates_list,
Well(3217): three_plates_list, Well(3223): three_plates_list,
Well(3229): three_plates_list, Well(3235): three_plates_list,
Well(3241): three_plates_list, Well(3247): three_plates_list,
Well(3265): three_plates_list, Well(3271): three_plates_list,
Well(3277): three_plates_list, Well(3283): three_plates_list,
Well(3289): three_plates_list, Well(3295): three_plates_list,
Well(3313): three_plates_list, Well(3319): three_plates_list,
Well(3325): three_plates_list, Well(3331): three_plates_list,
Well(3337): three_plates_list, Well(3343): three_plates_list,
Well(3361): three_plates_list, Well(3367): three_plates_list,
Well(3373): three_plates_list, Well(3379): three_plates_list,
Well(3385): three_plates_list, Well(3391): three_plates_list,
Well(3409): three_plates_list, Well(3415): three_plates_list,
Well(3421): three_plates_list, Well(3427): three_plates_list,
Well(3433): three_plates_list, Well(3439): three_plates_list,
Well(3457): three_plates_list, Well(3463): three_plates_list,
Well(3469): three_plates_list, Well(3475): three_plates_list,
Well(3481): three_plates_list, Well(3487): three_plates_list,
Well(3505): three_plates_list, Well(3511): three_plates_list,
Well(3517): three_plates_list, Well(3523): three_plates_list,
Well(3529): three_plates_list, Well(3535): three_plates_list,
Well(3553): three_plates_list, Well(3559): three_plates_list,
Well(3565): three_plates_list, Well(3571): three_plates_list,
Well(3577): three_plates_list, Well(3583): three_plates_list,
Well(3601): three_plates_list, Well(3607): three_plates_list,
Well(3613): three_plates_list, Well(3619): three_plates_list,
Well(3625): three_plates_list}
obs = tester.get_previously_plated_wells()
self.assertEqual(obs, exp)
# Create another plate and put a sample on it that isn't anywhere else
spp = SamplePlatingProcess.create(
User('*****@*****.**'), PlateConfiguration(1), 'New Plate For Prev')
spp.update_well(1, 1, '1.SKM1.640184')
obs = spp.plate.get_previously_plated_wells()
self.assertEqual(obs, {})
def integration_tests():
samples = get_samples()
user = User('*****@*****.**')
amplicon_seq_process = amplicon_workflow(user, samples)
shotgun_seq_process = shotgun_workflow(user, samples)
obs = amplicon_seq_process.generate_sample_sheet()
res = re.match(EXP_AMPLICON_SAMPLE_SHEET, obs)
if res is None:
raise ValueError(
'Amplicon sample sheet does not match expected regex:\n%s' % obs)
obs = shotgun_seq_process.generate_sample_sheet()
res = re.match(EXP_SHOTGUN_SAMPLE_SHEET, obs)
if res is None:
raise ValueError(
'Shotgun sample sheet does not match expected regex:\n%s' % obs)
def post(self):
username = self.get_argument('username', '').strip().lower()
passwd = self.get_argument('password', '')
error_msg = ""
user = None
try:
user = User.login(username, passwd)
except LabmanUnknownIdError:
error_msg = "Unknown user name"
except LabmanLoginError:
error_msg = "Incorrect password"
if user:
self.set_current_user(username)
self.redirect("/")
else:
self.render("index.html", message=error_msg, level='danger')
def test_list_users(self):
exp = [{
'email': '*****@*****.**',
'name': 'Admin'
}, {
'email': '*****@*****.**',
'name': 'Demo'
}, {
'email': '*****@*****.**',
'name': 'Dude'
}, {
'email': '*****@*****.**',
'name': '*****@*****.**'
}, {
'email': '*****@*****.**',
'name': 'Shared'
}]
self.assertEqual(User.list_users(), exp)
def test_get_previously_plated_wells(self):
tester = Plate(21)
self.assertEqual(tester.get_previously_plated_wells(), {})
# Create another plate and plate some samples in it
spp = SamplePlatingProcess.create(
User('*****@*****.**'), PlateConfiguration(1), 'New Plate For Prev')
spp.update_well(1, 1, '1.SKD1.640179')
exp = {}
plate = spp.plate
exp[Well(3208)] = [plate]
exp[Well(3388)] = [plate]
exp[Well(3568)] = [plate]
exp[Well(3748)] = [plate]
exp[Well(3928)] = [plate]
exp[Well(4108)] = [plate]
obs = tester.get_previously_plated_wells()
self.assertEqual(obs, exp)
def test_attributes(self):
s = Study(1)
self.assertEqual(s.title, 'Identification of the Microbiomes '
'for Cannabis Soils')
self.assertEqual(s.creator, User('*****@*****.**'))
self.assertEqual(s.num_samples, 27)
exp = {'num_samples': 27,
'number_samples_plated': 6,
'number_samples_extracted': 6,
'number_samples_amplicon_libraries': 6,
'number_samples_amplicon_pools': 6,
'number_samples_amplicon_sequencing_pools': 6,
'number_samples_amplicon_sequencing_runs': 6,
'number_samples_compressed': 6,
'number_samples_normalized': 6,
'number_samples_shotgun_libraries': 6,
'number_samples_shotgun_pool': 6,
'number_samples_shotgun_sequencing_runs': 6}
self.assertEqual(s.sample_numbers_summary, exp)
def test_attributes(self):
tester = User('*****@*****.**')
self.assertEqual(tester.name, 'Dude')
self.assertEqual(tester.email, '*****@*****.**')
def test_exist(self):
self.assertFalse(User.exists('*****@*****.**'))
self.assertTrue(User.exists('*****@*****.**'))
def test_hash_password(self):
obs = User._hash_password('password')
self.assertNotEqual(obs, 'password')
self.assertEqual(User._hash_password('password', obs), obs)
def test_init(self):
with self.assertRaises(LabmanUnknownIdError):
User('Dude')
def get(self):
self.render('access.html', users=User.list_users(),
access_users=User.list_users(access_only=True))
def setUp(self):
super().setUp()
self.user = User('*****@*****.**')
def test_sample_plating_process_handler_patch_request(self):
user = User('*****@*****.**')
# Test operation not supported
regex = ('Operation add not supported. Current supported '
'operations: replace')
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'add', '/well/8/1/sample', '1.SKM8.640201', None)
# Test incorrect path parameter
regex = 'Incorrect path parameter'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/8/1/', '1.SKM8.640201', None)
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample/content',
'1.SKM8.640201', None)
# Test attribute not found
regex = 'Attribute content not found'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/content/8/1/sample', '1.SKM8.640201',
None)
# Test well not found
regex = 'Well attribute WRONG not found'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/WRONG', '1.SKM8.640201', None)
# Test missing req_value
regex = 'A new value for the well should be provided'
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample', None, None)
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample', '', None)
with self.assertRaisesRegex(HTTPError, regex):
sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample', ' ', None)
# Test success
tester = SampleComposition(85)
self.assertEqual(tester.sample_composition_type, 'blank')
self.assertIsNone(tester.sample_id)
self.assertEqual(tester.content, 'blank.21.H1')
obs = sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample', '1.SKM8.640201', None)
self.assertEqual(tester.sample_composition_type, 'experimental sample')
self.assertEqual(tester.sample_id, '1.SKM8.640201')
self.assertEqual(tester.content, '1.SKM8.640201')
self.assertEqual(obs, {
'sample_id': '1.SKM8.640201',
'previous_plates': [],
'sample_ok': True
})
obs = sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample', 'Unknown', None)
self.assertEqual(tester.sample_composition_type, 'experimental sample')
self.assertIsNone(tester.sample_id)
self.assertEqual(tester.content, 'Unknown')
self.assertEqual(obs, {
'sample_id': 'Unknown',
'previous_plates': [],
'sample_ok': False
})
obs = sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/sample', 'blank', None)
self.assertEqual(tester.sample_composition_type, 'blank')
self.assertIsNone(tester.sample_id)
self.assertEqual(tester.content, 'blank.21.H1')
self.assertEqual(obs, {
'sample_id': 'blank.21.H1',
'previous_plates': [],
'sample_ok': True
})
# Test commenting a well
self.assertIsNone(tester.notes)
obs = sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/notes', 'New Notes', None)
self.assertEqual(tester.notes, 'New Notes')
obs = sample_plating_process_handler_patch_request(
user, 10, 'replace', '/well/8/1/notes', ' ', None)
self.assertIsNone(tester.notes)
def get(self):
pools = [[p['pool_composition_id'], p['external_id']]
for p in PoolComposition.list_pools()]
sequencers = Equipment.list_equipment('miseq')
self.render('sequencing.html', users=User.list_users(), pools=pools,
sequencers=sequencers)
def get_app(self):
BaseHandler.get_current_user = Mock(return_value=User("*****@*****.**"))
self.app.settings['debug'] = False
return self.app
