def run():
srcDir = DirectoryChooser("Choose directory").getDirectory()
if not srcDir:
return
targetDir = DirectoryChooser("Choose target directory").getDirectory()
if targetDir is None:
# User canceled the dialog
return
sId = ".tiff"
for root, directories, filenames in os.walk(srcDir):
for filename in filenames:
path = os.path.join(root, filename)
if not (sId in filename):
continue
cs = ChannelSeparator()
cs.setId(path)
print "cs", cs
bf = BFVirtualStack(path, cs, False, False, False)
for sliceIndex in xrange(1, bf.getSize() + 1):
print "Processing slice", sliceIndex
ip = bf.getProcessor(sliceIndex)
sliceFileName = os.path.join(targetDir + filename + "_" +
str(sliceIndex) + ".tiff")
print "writing ", sliceFileName
FileSaver(ImagePlus(str(sliceIndex),
ip)).saveAsTiff(sliceFileName)
def run(FolderName, SaveFolder):
# Find tiffiles in folder
onlyfiles = [f for f in os.listdir(FolderName) if
os.path.isfile(os.path.join(FolderName, f)) and f.lower().endswith('.tif')]
for ii in xrange(0, len(onlyfiles)):
path = os.path.join(FolderName, onlyfiles[ii])
print "Processing file..", path
# Find stimulus, Block, regions from filename for sorting
# Get all underscores
underscores = [m.start() for m in re.finditer('_', onlyfiles[ii])]
# Fish Name
findfish = onlyfiles[ii].find('Fish')
findunderscore = [i for i in underscores if i > findfish][0]
fishnum = onlyfiles[ii][findfish:findunderscore]
print 'Fish Number..', fishnum
# Block
findblock = onlyfiles[ii].find('Block')
findunderscore = [i for i in underscores if i > findblock][0]
blocknum = onlyfiles[ii][findblock:findunderscore]
print 'Block Number..', blocknum
# Stimulus
findstimulus = onlyfiles[ii].find('Blue')
findunderscore = [i for i in underscores if i > findstimulus][0]
stimulustype = onlyfiles[ii][findstimulus:findunderscore]
print 'Stimulus type..', stimulustype
# Region
findregion = onlyfiles[ii].find('Hb')
findunderscore = [i for i in underscores if i > findregion][0]
region = onlyfiles[ii][findregion-1:findunderscore]
print 'Region..', region
targetDir = os.path.join(SaveFolder, fishnum, region) + filesep
print 'Images will be saved in .....', targetDir
if not os.path.exists(targetDir):
os.makedirs(targetDir)
cs = ChannelSeparator()
cs.setId(path)
bf = BFVirtualStack(path, cs, False, False, False)
for sliceIndex in xrange(1,bf.getSize() + 1):
print "Processing slice", sliceIndex
ip = bf.getProcessor(sliceIndex)
sliceFileName = os.path.join(targetDir, "T=" + str(sliceIndex) + ".tif")
FileSaver(ImagePlus(str(sliceIndex), ip)).saveAsTiff(sliceFileName)
def dimensionsOf(path):
fr = None
try:
fr = ChannelSeparator()
fr.setGroupFiles(False)
fr.setId(path)
return fr.getSizeX(), fr.getSizeY()
except:
print sys.exc_info()
finally:
fr.close()
def bit_tester(image_name):
## gets the number of bits for an image, error if not 8 or 16 bit. ##
dest = IJ.getDirectory("image")
filepath = dest + image_name
fr = ChannelSeparator()
fr.setId(filepath)
bitDepth = fr.getBitsPerPixel()
if int(bitDepth) in (8, 16):
if int(bitDepth) == 8:
bit_depth = 2**(bitDepth)-1
if int(bitDepth) == 16:
bit_depth = 2**(bitDepth)-1
return bit_depth
elif int(bitDepth) not in (8, 16):
print("Error image is not 8 or 16 bit")
quit()
def run():
# Choose a file to open
od = OpenDialog("Choose multi-image file", None)
srcDir = od.getDirectory()
if srcDir is None:
# User canceled the dialog
return
path = os.path.join(srcDir, od.getFileName())
# Choose a directory to store each slice as a file
targetDir = DirectoryChooser("Choose target directory").getDirectory()
if targetDir is None:
# User canceled the dialog
return
# Ready:
cs = ChannelSeparator()
cs.setId(path)
print "cs", cs
bf = BFVirtualStack(path, cs, False, False, False)
for sliceIndex in xrange(1, bf.getSize() + 1):
print "Processing slice", sliceIndex
ip = bf.getProcessor(sliceIndex)
sliceFileName = os.path.join(targetDir, str(sliceIndex) + ".tif")
FileSaver(ImagePlus(str(sliceIndex), ip)).saveAsTiff(sliceFileName)
from loci.formats import ChannelSeparator
import os, sys
# Read the dimensions of the image at path by parsing the file header only,
# thanks to the LOCI Bioformats library
filepath = "/home/albert/Desktop/t2/bat-cochlea-volume.tif"
try:
fr = ChannelSeparator()
fr.setGroupFiles(False)
fr.setId(filepath)
width, height, nSlices = fr.getSizeX(), fr.getSizeY(), fr.getSizeZ()
n_pixels = width * height * nSlices
bitDepth = fr.getBitsPerPixel()
fileSize = os.path.getsize(filepath)
headerSize = fileSize - (n_pixels * bitDepth / 8) # 8 bits in 1 byte
print "Dimensions:", width, height, nSlices
print "Bit depth: %i-bit" % bitDepth
print "Likely header size, in number of bytes:", headerSize
except:
# Print the error, if any
print sys.exc_info()
finally:
fr.close() # close the file handle safely and always
# For parsing TIFF file headers
from java.io import RandomAccessFile
from java.math import BigInteger
