def test_internalize_conidia_0(macrophage_list: MacrophageCellList, grid: RectangularGrid, fungus_list: FungusCellList): m_det = 0 point = Point(x=35, y=35, z=35) macrophage_list.append(MacrophageCellData.create_cell(point=point)) fungus_list.append( FungusCellData.create_cell(point=point, status=FungusCellData.Status.RESTING)) vox = grid.get_voxel(macrophage_list[0]['point']) assert len(fungus_list.get_cells_in_voxel(vox)) == 1 f_index = int(fungus_list.get_cells_in_voxel(vox)) # 0 assert f_index == 0 fungus_list[f_index]['form'] = FungusCellData.Form.CONIDIA fungus_list[f_index]['status'] = FungusCellData.Status.RESTING macrophage_list.internalize_conidia(m_det, 50, 1, grid, fungus_list) assert grid.get_voxel(fungus_list[f_index]['point']) == vox assert fungus_list.cell_data['internalized'][f_index] assert macrophage_list.len_phagosome(0) == 1
def internalize_conidia(self, m_det, max_spores, p_in, grid, fungus: FungusCellList): for i in self.alive(): cell = self[i] vox = grid.get_voxel(cell['point']) # Moore neighborhood, but order partially randomized. Closest to furthest order, but # the order of any set of points of equal distance is random neighborhood = list( itertools.product(tuple(range(-1 * m_det, m_det + 1)), repeat=3)) shuffle(neighborhood) neighborhood = sorted(neighborhood, key=lambda v: v[0]**2 + v[1]**2 + v[2]**2) for dx, dy, dz in neighborhood: zi = vox.z + dz yj = vox.y + dy xk = vox.x + dx if grid.is_valid_voxel(Voxel(x=xk, y=yj, z=zi)): index_arr = fungus.get_cells_in_voxel( Voxel(x=xk, y=yj, z=zi)) for index in index_arr: if (fungus[index]['form'] == FungusCellData.Form.CONIDIA and not fungus[index]['internalized'] and p_in > rg.random()): fungus[index]['internalized'] = True self.append_to_phagosome(i, index, max_spores)
def produce_cytokines(self, m_det, m_n, grid, fungus: FungusCellList, cyto): for i in self.alive(): vox = grid.get_voxel(self[i]['point']) hyphae_count = 0 # Moore neighborhood neighborhood = tuple( itertools.product(tuple(range(-1 * m_det, m_det + 1)), repeat=3)) for dx, dy, dz in neighborhood: zi = vox.z + dz yj = vox.y + dy xk = vox.x + dx if grid.is_valid_voxel(Voxel(x=xk, y=yj, z=zi)): index_arr = fungus.get_cells_in_voxel( Voxel(x=xk, y=yj, z=zi)) for index in index_arr: if fungus[index]['form'] == FungusCellData.Form.HYPHAE: hyphae_count += 1 cyto[vox.z, vox.y, vox.x] = cyto[vox.z, vox.y, vox.x] + m_n * hyphae_count
def damage_hyphae(self, n_det, n_kill, time, health, grid, fungus: FungusCellList, iron): for i in self.alive(self.cell_data['granule_count'] > 0): cell = self[i] vox = grid.get_voxel(cell['point']) # Moore neighborhood, but order partially randomized. Closest to furthest order, but # the order of any set of points of equal distance is random neighborhood = list(itertools.product(tuple(range(-1 * n_det, n_det + 1)), repeat=3)) shuffle(neighborhood) neighborhood = sorted(neighborhood, key=lambda v: v[0] ** 2 + v[1] ** 2 + v[2] ** 2) for dx, dy, dz in neighborhood: zi = vox.z + dz yj = vox.y + dy xk = vox.x + dx if grid.is_valid_voxel(Voxel(x=xk, y=yj, z=zi)): index_arr = fungus.get_cells_in_voxel(Voxel(x=xk, y=yj, z=zi)) if len(index_arr) > 0: iron[zi, yj, xk] = 0 for index in index_arr: if ( fungus[index]['form'] == FungusCellData.Form.HYPHAE and cell['granule_count'] > 0 ): fungus[index]['health'] -= health * (time / n_kill) cell['granule_count'] -= 1 cell['status'] = NeutrophilCellData.Status.GRANULATING elif cell['granule_count'] == 0: cell['status'] = NeutrophilCellData.Status.NONGRANULATING break
def test_internalize_conidia_none( populated_epithelium: EpitheliumCellList, grid: RectangularGrid, fungus_list: FungusCellList, ): cell = populated_epithelium[0] vox = grid.get_voxel(cell['point']) assert len(fungus_list.get_cells_in_voxel(vox)) == 0 populated_epithelium.internalize_conidia(0, 10, 1, grid, fungus_list) assert populated_epithelium.len_phagosome(0) == 0 for v in cell['phagosome']: assert v == -1
def test_internalize_conidia_none( populated_macrophage: MacrophageCellList, grid: RectangularGrid, populated_fungus: FungusCellList, ): m_det = 0 cell = populated_macrophage[0] vox = grid.get_voxel(cell['point']) assert len(populated_fungus.get_cells_in_voxel(vox)) == 1 populated_macrophage.internalize_conidia(m_det, 50, 1, grid, populated_fungus) assert populated_macrophage.len_phagosome(0) == 0 for v in cell['phagosome']: assert v == -1