def bcalm(input_filename, output_filename, k, m): input_file = open(input_filename) simple_buckets = False if simple_buckets: minimizers = sorted(list(set(map(lambda s: minimizer("".join(s), m), product("acgt", repeat=m))))) buckets = Buckets(minimizers, output_filename) else: m *= 2 minimizers = sorted(list(set(map(lambda s: minimizer("".join(s), m), product("acgt", repeat=m))))) buckets = Superbuckets(minimizers, output_filename) precompute_hashes(m) # partition k-mers for line in input_file: kmer = line.strip()[:-1] bucket_minimizer = minimizer(kmer, m) buckets.put(kmer, bucket_minimizer) buckets.flush() buckets.stats() # process each bucket in minimizer order for bucket_file, bucket_minimizer in buckets.iterate(): G = Graph(k) buckets.flush() G.importg(bucket_file) G.debruijn() G.compress(bucket_minimizer, m) for node in G.nodes.values(): if use_tags: node = untag(node) min = minbutbiggerthan(node[: k - 1], node[-(k - 1) :], bucket_minimizer, m) buckets.put(node, min)
def naive(input, output): G = Graph(k) G.importg(input) G.debruijn() G.compress() G.output(output) sys.exit()