def test_holding_at_target():
tc = Thermocycler(lambda x: None)
tc._target_temp = 30
tc._current_temp = 30.1
# not yet stabilized
tc._block_temp_buffer = [26, 28, 29, 30, 30.5, 29.8, 30.25, 29.9, 30, 30.1]
assert not tc._is_holding_at_target()
# stabilized at target
tc._block_temp_buffer = [30.25, 29.9, 30.0, 30.1, 30, 30, 30, 30, 30, 30.1]
assert tc._is_holding_at_target()
# not enough history
tc._block_temp_buffer = [29.8, 30, 30, 30.1]
assert not tc._is_holding_at_target()
async def test_set_block_temperature():
# set the block target temperature
tc = Thermocycler(lambda x: None)
command_log = []
tc._target_temp = 25
tc._current_temp = 25
async def _mock_write_and_wait(self, command):
nonlocal command_log
command_log.append(command)
return command
tc._write_and_wait = types.MethodType(_mock_write_and_wait, tc)
# basic set block temp
await tc.set_temperature(21)
assert command_log.pop(0) == 'M104 S21'
# upper clamp set block temp
await tc.set_temperature(130)
assert command_log.pop(0) == 'M104 S99'
# lower clamp set block temp
await tc.set_temperature(-30)
assert command_log.pop(0) == 'M104 S0'
# hold set block temp
await tc.set_temperature(21, hold_time=1)
assert command_log.pop(0) == 'M104 S21 H1'
# volume set block temp
await tc.set_temperature(21, volume=75)
assert command_log.pop(0) == 'M104 S21 V75'
# hold and volume set block temp
await tc.set_temperature(21, hold_time=1, volume=75)
assert command_log.pop(0) == 'M104 S21 H1 V75'
# ramp rate set block temp
await tc.set_temperature(21, ramp_rate=3)
assert command_log.pop(0) == 'M566 S3'
assert command_log.pop(0) == 'M104 S21'
async def test_deactivates():
tc = Thermocycler(lambda x: None)
command_log = []
tc._target_temp = 25
tc._current_temp = 25
async def _mock_write_and_wait(self, command):
nonlocal command_log
command_log.append(command)
return command
tc._write_and_wait = types.MethodType(_mock_write_and_wait, tc)
await tc.deactivate_all()
assert command_log.pop(0) == 'M18'
await tc.deactivate_lid()
assert command_log.pop(0) == 'M108'
await tc.deactivate_block()
assert command_log.pop(0) == 'M14'
async def test_set_temperature_with_fuzzy_hold_time():
tc = Thermocycler(lambda x: None)
command_log = []
async def _mock_write_and_wait(self, command):
nonlocal command_log
command_log.append(command)
return command
tc._write_and_wait = types.MethodType(_mock_write_and_wait, tc)
tc._current_temp = 21
tc._target_temp = 21
# set block temp with same temp as previous, different hold time
tc._hold_time = 48 # Hold time returned by thermocycler
try:
await tc.set_temperature(21, hold_time=50)
except ThermocyclerError:
pytest.fail("Fuzzy hold_time test failed")
assert command_log.pop(0) == 'M104 S21 H50'
# set block temp with same temp and different hold time. But serial poller
# did not read back from thermocycler within HOLD_TIME_FUZZY_SECONDS
tc._hold_time = 0
expected_error = "Thermocycler driver set the block temp to T=21 & " \
"H=40 but status reads T=21 & H=0"
with pytest.raises(ThermocyclerError, match=expected_error):
await tc.set_temperature(21, hold_time=40)
assert command_log.pop(0) == 'M104 S21 H40'
# set block temp with same temp but hold_time < HOLD_TIME_FUZZY_SECONDS
# will return immediately regardless of no hold_time update read.
tc._hold_time = 0 # Hold time from previous set_temperature cmd
try:
await tc.set_temperature(21, hold_time=5)
except ThermocyclerError:
pytest.fail("Fuzzy hold_time test (with <=5 sec hold_time) failed")
assert command_log.pop(0) == 'M104 S21 H5'
async def test_set_block_temperature():
# set the block target temperature
tc = Thermocycler(lambda x: None)
command_log = []
tc._target_temp = 25
tc._current_temp = 25
async def _mock_write_and_wait(self, command):
nonlocal command_log
command_log.append(command)
return command
tc._write_and_wait = types.MethodType(_mock_write_and_wait, tc)
# raises error if unable to verify temp has been set
expected_error = "Thermocycler driver set the block temp to T=21 & " \
"H=None but status reads T=25 & H=None"
with pytest.raises(ThermocyclerError, match=expected_error):
await tc.set_temperature(21)
assert command_log.pop(0) == 'M104 S21'
# basic set block temp
tc._target_temp = 21
await tc.set_temperature(21)
assert command_log.pop(0) == 'M104 S21'
# upper clamp set block temp
tc._target_temp = 99.0
await tc.set_temperature(130)
assert command_log.pop(0) == 'M104 S99.0'
# lower clamp set block temp
tc._target_temp = 0.0
await tc.set_temperature(-30)
assert command_log.pop(0) == 'M104 S0.0'
# hold set block temp
tc._target_temp = 21
tc._hold_time = 1
await tc.set_temperature(21, hold_time=1)
assert command_log.pop(0) == 'M104 S21 H1'
tc._hold_time = None
# volume set block temp
tc._target_temp = 21
await tc.set_temperature(21, volume=75)
assert command_log.pop(0) == 'M104 S21 V75'
# hold and volume set block temp
tc._target_temp = 21
tc._hold_time = 1
await tc.set_temperature(21, hold_time=1, volume=75)
assert command_log.pop(0) == 'M104 S21 H1 V75'
tc._hold_time = None
# ramp rate set block temp
tc._target_temp = 21
await tc.set_temperature(21, ramp_rate=3)
assert command_log.pop(0) == 'M566 S3'
assert command_log.pop(0) == 'M104 S21'