def quality_control_matches(matches, probes, dupes, k, v, verbose=False):
"""check to make sure we don't get any more matches than expected
and that matches are reasonably close to each other on their respective
chromos"""
skip = False
chromo, strand, start, end = None, None, None, None
if len(v) > 1:
if run_checks(k, v, probes):
# sort by match position
v_sort = sorted(v, key=itemgetter(2))
start, end = v_sort[0][2], v_sort[-1][3]
diff = end - start
# ensure our range is less than N(probes) * probe_length - this
# still gives us a little wiggle room because probes are ~ 2X tiled
if diff > (probes[k] * 140):
skip = True
if verbose:
print "range longer than expected"
else:
chromo = v[0][0]
strand = v[0][1]
else:
skip = True
elif k in dupes:
skip = True
print "{0} is in dupefile".format(k)
else:
chromo, strand, start, end = v[0]
return chromo, strand, start, end, skip
def quality_control_matches(matches, probes, dupes, k, v, verbose=False):
"""check to make sure we don't get any more matches than expected
and that matches are reasonably close to each other on their respective
chromos"""
skip = False
chromo, strand, start, end = None, None, None, None
if len(v) > 1:
if run_checks(k, v, probes):
# sort by match position
v_sort = sorted(v, key=itemgetter(2))
start, end = v_sort[0][2], v_sort[-1][3]
diff = end - start
# ensure our range is less than N(probes) * probe_length - this
# still gives us a little wiggle room because probes are ~ 2X tiled
if diff > (probes[k] * 140):
skip = True
if verbose:
print "range longer than expected"
else:
chromo = v[0][0]
strand = v[0][1]
else:
skip = True
elif k in dupes:
skip = True
print "{0} is in dupefile".format(k)
else:
chromo, strand, start, end = v[0]
return chromo, strand, start, end, skip
def main():
args = get_args()
uces = set([
get_name(read.identifier, "|", 1)
for read in fasta.FastaReader(args.query)
])
files = glob.glob(os.path.join(args.lastz, '*.lastz'))
# this prob. needs to be more robust
organisms = [
os.path.splitext(os.path.basename(f).split('-')[-1])[0].replace(
'-', "_") for f in files
]
conn, c = create_match_database(args.db, organisms, uces)
if args.dupefile:
dupes = get_dupes(args.dupefile)
else:
dupes = None
#pdb.set_trace()
for f in files:
critter = os.path.splitext(os.path.basename(f).split('-')[-1])[0]
matches, probes = get_matches(f, args.splitchar, args.components)
count = 0
for k, v in matches.iteritems():
skip = False
if len(v) > 1:
if run_checks(k, v, probes, args.verbose):
# sort by match position
v_sort = sorted(v, key=itemgetter(2))
start, end = v_sort[0][2], v_sort[-1][3]
diff = end - start
# ensure our range is less than N(probes) * probe_length - this
# still gives us a little wiggle room because probes are ~ 2X tiled
if diff > (probes[k] * 120):
skip = True
if args.verbose:
print "range longer than expected"
else:
skip = True
elif args.dupefile and k in dupes:
skip = True
if args.verbose: print "{0} is in dupefile".format(k)
else:
pass
if not skip:
store_lastz_results_in_db(c, critter, k)
count += 1
print "Entered {} matches for {}".format(count, critter)
conn.commit()
c.close()
conn.close()
def main():
args = get_args()
uces = set([get_name(read.identifier, "|", 1) for read in fasta.FastaReader(args.query)])
files = glob.glob(os.path.join(args.lastz, '*.lastz'))
# this prob. needs to be more robust
organisms = [os.path.splitext(os.path.basename(f).split('-')[-1])[0].replace('-',"_") for f in files]
conn, c = create_match_database(args.db, organisms, uces)
if args.dupefile:
dupes = get_dupes(args.dupefile)
else:
dupes = None
#pdb.set_trace()
for f in files:
critter = os.path.splitext(os.path.basename(f).split('-')[-1])[0]
matches, probes = get_matches(f, args.splitchar, args.components)
count = 0
for k,v in matches.iteritems():
skip = False
if len(v) > 1:
if run_checks(k, v, probes, args.verbose):
# sort by match position
v_sort = sorted(v, key = itemgetter(2))
start, end = v_sort[0][2], v_sort[-1][3]
diff = end - start
# ensure our range is less than N(probes) * probe_length - this
# still gives us a little wiggle room because probes are ~ 2X tiled
if diff > (probes[k] * 120):
skip = True
if args.verbose:
print "range longer than expected"
else:
skip = True
elif args.dupefile and k in dupes:
skip = True
if args.verbose:print "{0} is in dupefile".format(k)
else:
pass
if not skip:
store_lastz_results_in_db(c, critter, k)
count += 1
print "Entered {} matches for {}".format(count, critter)
conn.commit()
c.close()
conn.close()
