def startFrame():
    # Read a frame from feed
    global frame
    ret, frame = cap.read()
    # Convert to greyscale frame
    global frame_gray
    frame_gray = cv2.cvtColor(frame, cv2.COLOR_BGR2GRAY)
    # Get histogram of frame
    hist_img = cv2.calcHist([frame], [0], None, [256], [0, 256])
    #plt.plot(hist_img)
    #plt.show()
    
    # Pass frame to histogram adjustment to remove ouliers
    hist_no_outliers, lower_index = outliers.removeOutliersThresh(hist_img)
    #plt.plot(hist_no_outliers)
    #plt.show()
    
    # Pass histogram to adaptive thresholding to determine level
    global threshLevel
    threshLevel = thresh.bi_level_img_threshold(hist_no_outliers)
    
    # Adjust start index of hist and add manual level adjustment
    global threshLevelAdjust
    threshLevelAdjust = threshLevel + lower_index + 15
    #print('Bi level thresh', threshLevelAdjust)
    
    struct_el = cv2.getStructuringElement(cv2.MORPH_ELLIPSE,(25,25))
    global frame_open
    frame_open = cv2.morphologyEx(frame_gray, cv2.MORPH_OPEN, struct_el)
    #cv2.imshow('open',frame_open)

    return frame_open
	
#while(cap.isOpened()):

    # Read a frame from feed
    # ret, frame = cap.read()
    # Convert to greyscale frame
frame_gray = cv2.cvtColor(frame, cv2.COLOR_BGR2GRAY)
i = i+1
print('i ', i)
    # Get histogram of frame
hist_img = cv2.calcHist([frame_gray], [0], None, [256], [0, 256])
    #plt.plot(hist_img)
    #plt.show()

    # Pass frame to histogram adjustment to remove ouliers
hist_no_outliers, lower_index = outliers.removeOutliersThresh(hist_img)
    #plt.plot(hist_no_outliers)
    #plt.show()

    # Pass histogram to adaptive thresholding to determine level
threshLevel = thresh.bi_level_img_threshold(hist_no_outliers)

    # Adjust start index of hist and add manual level adjustment
threshLevelAdjust = 40 #threshLevel + lower_index 
print('Bi level thresh', threshLevelAdjust)

plt.plot(hist_no_outliers)
plt.show()

struct_el = cv2.getStructuringElement(cv2.MORPH_ELLIPSE,(25,25))
frame_open = cv2.morphologyEx(frame_gray, cv2.MORPH_OPEN, struct_el)