def import_contig_lengths(contigs_fasta, log_file, quiet):
message = 'Gathering contig lengths from {0}.'.format(contigs_fasta)
shared.give_user_feedback(message, log_file, quiet)
contig2length = {}
with open(contigs_fasta, 'r') as f1:
for line in f1:
line = line.rstrip()
if line.startswith('>'):
contig = line.split(' ')[0].lstrip('>')
contig2length[contig] = 0
else:
try:
contig2length[contig] += len(line)
except:
message = '{0} is not a contigs fasta'.format(
contigs_fasta)
shared.give_user_feedback(message,
log_file,
quiet,
error=True)
sys.exit(1)
return contig2length
def find_offspring(taxonomy_folder, fastaid2LCAtaxid_file, log_file, quiet):
nodes_dmp = '{0}/nodes.dmp'.format(taxonomy_folder)
(taxid2parent, taxid2rank) = tax.import_nodes(nodes_dmp, log_file, quiet)
message = 'Searching nr database for taxids with multiple offspring.'
shared.give_user_feedback(message, log_file, quiet)
taxid2offspring = {}
with open(fastaid2LCAtaxid_file, 'r') as f1:
for line in f1:
line = line.rstrip().split('\t')
taxid = line[1]
lineage = tax.find_lineage(taxid, taxid2parent)
for (i, taxid) in enumerate(lineage):
# The first taxid in the lineage does not have a daughter node.
if i == 0:
continue
if taxid not in taxid2offspring:
taxid2offspring[taxid] = set()
offspring = lineage[i - 1]
taxid2offspring[taxid].add(offspring)
return taxid2offspring
def import_prot_accession2taxid(prot_accession2taxid_file,
prot_accessions_whitelist, log_file, quiet):
message = 'Loading file {0}.'.format(prot_accession2taxid_file)
shared.give_user_feedback(message, log_file, quiet)
prot_accession2taxid = {}
with gzip.open(prot_accession2taxid_file, 'rb') as f1:
for n, line in enumerate(f1):
line = line.decode('utf-8')
line = line.rstrip().split('\t')
if n == 0:
index_1 = line.index('accession.version')
index_2 = line.index('taxid')
continue
prot_accession = line[index_1]
if prot_accession in prot_accessions_whitelist:
prot_accession2taxid[prot_accession] = line[index_2]
return prot_accession2taxid
def make_fastaid2LCAtaxid_file(nodes_dmp, fastaid2LCAtaxid_file, nr_file,
prot_accession2taxid_file, taxid2parent,
log_file, quiet):
(fastaid2prot_accessions,
prot_accessions_whitelist) = import_headers_nr(nr_file, log_file, quiet)
prot_accession2taxid = import_prot_accession2taxid(
prot_accession2taxid_file, prot_accessions_whitelist, log_file, quiet)
message = 'Finding LCA of all protein accession numbers in fasta headers.'
shared.give_user_feedback(message, log_file, quiet)
no_taxid = 0
corrected = 0
total = 0
with open(fastaid2LCAtaxid_file, 'w') as outf1:
for fastaid, prot_accessions in fastaid2prot_accessions.items():
list_of_lineages = []
for prot_accession in prot_accessions:
try:
taxid = prot_accession2taxid[prot_accession]
lineage = tax.find_lineage(taxid, taxid2parent)
list_of_lineages.append(lineage)
except:
# This accounts for missing accession numbers in
# prot.accession2taxid and missing nodes in nodes.dmp.
continue
total += 1
if len(list_of_lineages) == 0:
# This accounts for entries that only contain accession numbers
# that are missing in prot.accession2taxid or whose taxid is
# missing in nodes.dmp. NOTE that these entries are thus not
# present in the output file.
no_taxid += 1
continue
LCAtaxid = tax.find_LCA(list_of_lineages)
outf1.write('{0}\t{1}\n'.format(fastaid, LCAtaxid))
if (fastaid not in prot_accession2taxid
or LCAtaxid != prot_accession2taxid[fastaid]):
# If the fastaid cannot be found in prot.accession2taxid, but
# a taxid is given to the fastaid based on secondary accession
# numbers, or if the taxid of the header is different from the
# LCA taxid, it is counted as corrected.
corrected += 1
message = (
'Done! File {0} is created. '
'{1:,d} of {2:,d} headers ({3:.1f}%) corrected. '
'{4:,d} headers ({5:.1f}%) do not have a taxid assigned.'.format(
fastaid2LCAtaxid_file, corrected, total, corrected / total * 100,
no_taxid, no_taxid / total * 100))
shared.give_user_feedback(message, log_file, quiet)
return
def write_taxids_with_multiple_offspring_file(
taxids_with_multiple_offspring_file, taxid2offspring, log_file, quiet):
message = 'Writing {0}.'.format(taxids_with_multiple_offspring_file)
shared.give_user_feedback(message, log_file, quiet)
with open(taxids_with_multiple_offspring_file, 'w') as outf1:
for taxid in taxid2offspring:
if len(taxid2offspring[taxid]) >= 2:
outf1.write('{0}\n'.format(taxid))
def check_in_and_output_file(input_file, output_file, log_file, quiet):
error = False
if input_file == output_file:
message = 'input file and output file can not be the same.'
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_input_file(input_file, log_file, quiet):
error = False
if not os.path.isfile(input_file):
message = 'ERROR: input file {0} does not exist.'.format(input_file)
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_whether_ORFs_are_based_on_contigs(contig_names, contig2ORFs,
log_file, quiet):
for contig in contig2ORFs:
if contig not in contig_names:
message = ('ERROR: found a protein in the predicted proteins '
'fasta file that can not be traced back to one of the '
'contigs in the contigs fasta file: {0}. Proteins '
'should be named contig_name_#.'
''.format(contig2ORFs[contig][0]))
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
def check_output_file(output_file, log_file, quiet):
error = False
if os.path.isfile(output_file):
message = ('ERROR: output file {0} already exists. You can choose to '
'overwrite existing files with the [--force] argument.'
''.format(output_file))
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_md5_gz(gz_file, md5_file, log_file, quiet):
message = 'Checking file integrity via MD5 checksum.'
shared.give_user_feedback(message, log_file, quiet)
with open(md5_file, 'r') as f:
md5_exp = f.read().split(' ')[0]
if md5_exp == '':
message = ('WARNING: no MD5 found in {0}. Integrity of {1} can not be '
'established.'.format(md5_file, gz_file))
shared.give_user_feedback(message, log_file, quiet)
else:
md5 = hashlib.md5()
block_size = 4096
with open(gz_file, 'rb') as f:
for chunk in iter(lambda: f.read(block_size), b''):
md5.update(chunk)
md5 = md5.hexdigest()
if md5 != md5_exp:
message = 'MD5 of {0} does not check out.'.format(gz_file)
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
else:
message = 'MD5 of {0} checks out.'.format(gz_file)
shared.give_user_feedback(message, log_file, quiet)
return
def download_nr(nr_file, log_file, quiet):
url = 'ftp://ftp.ncbi.nlm.nih.gov/blast/db/FASTA/'
message = 'Downloading nr database from {0} to database folder.'.format(
url)
shared.give_user_feedback(message, log_file, quiet)
url = '{0}nr.gz'.format(url)
try:
urllib.request.urlretrieve(url, nr_file)
except:
message = 'download of {0} failed.'.format(url)
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
url = '{0}.md5'.format(url)
md5_file = '{0}.md5'.format(nr_file)
try:
urllib.request.urlretrieve(url, md5_file)
except:
message = 'download of {0} failed.'.format(url)
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
message = 'Download complete.'
shared.give_user_feedback(message, log_file, quiet)
check.check_md5_gz(nr_file, md5_file, log_file, quiet)
return
def download_taxonomy_files(taxonomy_folder, date, log_file, quiet):
url = 'ftp://ftp.ncbi.nlm.nih.gov/pub/taxonomy/taxdump.tar.gz'
tmp_taxonomy_file = '{0}/{1}.taxdump.tar.gz'.format(taxonomy_folder, date)
message = ('Downloading and extracting taxonomy files from {0} to {1}.'
''.format(url, taxonomy_folder))
shared.give_user_feedback(message, log_file, quiet)
try:
urllib.request.urlretrieve(url, tmp_taxonomy_file)
except:
message = 'ERROR: donwload of taxonomy files failed.'
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
try:
with tarfile.open(tmp_taxonomy_file) as tar:
tar.extractall(taxonomy_folder)
except:
message = ('ERROR: something went wrong while extracting the taxonomy '
'files.')
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
message = 'Download complete!'
shared.give_user_feedback(message, log_file, quiet)
def download_prot_accession2taxid_file(prot_accession2taxid_file, date,
log_file, quiet):
url = 'ftp://ftp.ncbi.nlm.nih.gov/pub/taxonomy/accession2taxid/'
message = 'Downloading mapping file from {0} to taxonomy folder.'.format(
url)
shared.give_user_feedback(message, log_file, quiet)
url = '{0}prot.accession2taxid.FULL.gz'.format(url)
try:
urllib.request.urlretrieve(url, prot_accession2taxid_file)
except:
message = 'download of {0} failed.'.format(url)
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
url = '{0}.md5'.format(url)
md5_file = '{0}.md5'.format(prot_accession2taxid_file)
try:
urllib.request.urlretrieve(url, md5_file)
except:
message = 'download of {0} failed.'.format(url)
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
message = 'Download complete.'
shared.give_user_feedback(message, log_file, quiet)
check.check_md5_gz(prot_accession2taxid_file, md5_file, log_file, quiet)
return
def import_taxids_with_multiple_offspring(taxids_with_multiple_offspring_file,
log_file, quiet):
message = 'Importing file {0}.'.format(taxids_with_multiple_offspring_file)
shared.give_user_feedback(message, log_file, quiet)
taxids_with_multiple_offspring = set()
with open(taxids_with_multiple_offspring_file, 'r') as f1:
for line in f1:
line = line.rstrip()
taxids_with_multiple_offspring.add(line)
return taxids_with_multiple_offspring
def import_prot_accession2taxid(prot_accession2taxid_file, log_file, quiet):
message = ('Loading {0} into memory. Please be patient...'
''.format(prot_accession2taxid_file))
shared.give_user_feedback(message, log_file, quiet)
prot_accession2taxid = {}
with gzip.open(prot_accession2taxid_file, 'rt') as f1:
for line in f1:
line = line.split('\t')
prot_accession2taxid[line[1]] = line[2]
return prot_accession2taxid
def import_fastaid2LCAtaxid(fastaid2LCAtaxid_file, all_hits, log_file, quiet):
message = 'Importing file {0}.'.format(fastaid2LCAtaxid_file)
shared.give_user_feedback(message, log_file, quiet)
fastaid2LCAtaxid = {}
with shared.open_maybe_gzip(fastaid2LCAtaxid_file, 'rt') as f1:
for line in f1:
line = line.rstrip().split('\t')
if line[0] in all_hits:
# Only include fastaids that are found in hits.
fastaid2LCAtaxid[line[0]] = line[1]
return fastaid2LCAtaxid
def check_top(top, r, log_file, quiet):
error = False
if top < 50:
message = ('WARNING: [--top] is set lower than 50. This might '
'conflict with future runs with higher settings of the '
'[-r / --range] parameter, see README.md.')
shared.give_user_feedback(message, log_file, quiet)
if top <= r:
message = 'ERROR: [--top] should be higher than [-r / --range].'
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_bin_fasta(bin_fasta, log_file, quiet):
error = False
if check_fasta(bin_fasta, log_file, quiet):
error = True
if os.path.isdir(bin_fasta):
message = (
'{0} is a directory. If you want to classify more than 1 bin '
'you can run \'CAT bins\' instead of \'CAT bin\'.'.format(
bin_fasta))
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def make_concatenated_fasta(concatenated_fasta, bin2contigs, bin_folder,
log_file, quiet):
message = 'Writing {0}.'.format(concatenated_fasta)
shared.give_user_feedback(message, log_file, quiet)
with open(concatenated_fasta, 'w') as outf1:
for bin_ in sorted(bin2contigs):
with open('{0}/{1}'.format(bin_folder, bin_), 'r') as f1:
for line in f1:
if line.startswith('>'):
contig = line.split(' ')[0].rstrip().lstrip('>')
# add bin name in front of the contig name.
outf1.write('>{0}_{1}\n'.format(bin_, contig))
else:
outf1.write(line)
def import_names(names_dmp, log_file, quiet):
message = 'Importing file {0}.'.format(names_dmp)
shared.give_user_feedback(message, log_file, quiet)
taxid2name = {}
with shared.open_maybe_gzip(names_dmp, 'rt') as f1:
for line in f1:
line = line.split('\t')
if line[6] == 'scientific name':
taxid = line[0]
name = line[2]
taxid2name[taxid] = name
return taxid2name
def memory_bottleneck(args):
(total_memory, error) = check.check_memory(args.min_mem)
if error:
message = ('at least {0}GB of memory is needed for the database '
'construction. {1}GB is found on your system. You can try '
'to find a machine with more memory, or download '
'preconstructed database files from '
'tbb.bio.uu.nl/bastiaan/CAT_prepare/.'.format(
args.min_mem, total_memory))
shared.give_user_feedback(message,
args.log_file,
args.quiet,
error=True)
sys.exit(1)
return
def check_bin_fasta(bin_fasta, log_file, quiet):
error = False
if not check_whether_file_is_fasta(bin_fasta):
message = 'ERROR: {0} is not a fasta file.'.format(bin_fasta)
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
if os.path.isdir(bin_fasta):
message = ('ERROR: {0} is a directory. If you want to classify more '
'than 1 bin you can run \'CAT bins\' instead of '
'\'CAT bin\'.'.format(bin_fasta))
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_prodigal_binaries(path_to_prodigal, log_file, quiet):
error = False
try:
p = subprocess.Popen([path_to_prodigal, '-v'], stderr=subprocess.PIPE)
c = p.communicate()
output = c[1].decode().rstrip().lstrip()
message = 'Prodigal found: {0}.'.format(output)
shared.give_user_feedback(message, log_file, quiet)
except OSError:
message = ('ERROR: can not find Prodigal. Please check whether it is '
'installed or path to the binaries is provided.')
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_diamond_binaries(path_to_diamond, log_file, quiet):
error = False
try:
p = subprocess.Popen([path_to_diamond, '--version'],
stdout=subprocess.PIPE)
c = p.communicate()
output = c[0].decode().rstrip()
message = 'DIAMOND found: {0}.'.format(output)
shared.give_user_feedback(message, log_file, quiet)
except OSError:
message = ('ERROR: can not find DIAMOND. Please check whether it is '
'installed or path to the binaries is provided.')
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def import_nodes(nodes_dmp, log_file, quiet):
message = 'Importing file {0}.'.format(nodes_dmp)
shared.give_user_feedback(message, log_file, quiet)
taxid2parent = {}
taxid2rank = {}
with open(nodes_dmp, 'r') as f1:
for line in f1:
line = line.split('\t')
taxid = line[0]
parent = line[2]
rank = line[4]
taxid2parent[taxid] = parent
taxid2rank[taxid] = rank
return (taxid2parent, taxid2rank)
def check_fasta(file_, log_file, quiet):
error = False
if not os.path.isfile(file_):
error = True
else:
with open(file_, 'r') as f1:
for n, line in enumerate(f1):
if n == 0:
if not line.startswith('>'):
error = True
break
if error:
message = '{0} is not a fasta file.'.format(file_)
shared.give_user_feedback(message, log_file, quiet, error=True)
return error
def check_out_prefix(out_prefix, log_file, quiet):
error = False
if '/' in out_prefix:
if out_prefix.endswith('/'):
message = ('ERROR: prefix for output files ({0}) appears to be a '
'directory.'.format(out_prefix))
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
directory = out_prefix.rsplit('/', 1)[0]
if not os.path.isdir(directory):
message = ('ERROR: can not find output directory {0} to which '
'output files should be written.'.format(directory))
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def check_out_prefix(out_prefix, log_file, quiet):
error = False
if os.path.isdir(out_prefix):
message = 'prefix for output files ({0}) is a directory.'.format(
out_prefix)
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
dir_ = out_prefix.rsplit('/', 1)[0]
if not os.path.isdir(dir_):
message = ('can not find output directory {0} to which output files '
'should be written.'.format(dir_))
shared.give_user_feedback(message, log_file, quiet, error=True)
error = True
return error
def make_diamond_database(path_to_diamond,
nr_file,
diamond_database_prefix,
nproc,
log_file,
quiet):
message = ('Constructing DIAMOND database {0}.dmnd from {1} '
'using {2} cores. Please be patient...'
''.format(diamond_database_prefix, nr_file, nproc))
shared.give_user_feedback(message, log_file, quiet)
command = [path_to_diamond, 'makedb',
'--in', nr_file,
'-d', diamond_database_prefix,
'-p', str(nproc),
'--quiet']
try:
subprocess.check_call(command)
except:
message = 'ERROR: DIAMOND database could not be created.'
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
message = 'DIAMOND database constructed!'
shared.give_user_feedback(message, log_file, quiet)
def make_diamond_database(path_to_diamond, nr_file, diamond_database_prefix,
nproc, log_file, quiet, verbose):
message = ('Constructing DIAMOND database {0}.dmnd from {1} using {2} '
'cores.'.format(diamond_database_prefix, nr_file, nproc))
shared.give_user_feedback(message, log_file, quiet)
command = [
path_to_diamond, 'makedb', '--in', nr_file, '-d',
diamond_database_prefix, '-p',
str(nproc)
]
if not verbose:
command += ['--quiet']
try:
subprocess.check_call(command)
except:
message = 'DIAMOND database could not be created.'
shared.give_user_feedback(message, log_file, quiet, error=True)
sys.exit(1)
message = 'DIAMOND database constructed.'
shared.give_user_feedback(message, log_file, quiet)
return
