# Create a copy of the image. input_image_plus_copy = input_image_plus.duplicate() image_processor_copy = input_image_plus_copy.getProcessor() try: # Set binary options. options = jython_utils.get_binary_options( black_background=black_background) IJ.run(input_image_plus_copy, "Options...", options) # Convert image to binary if necessary. if not image_processor_copy.isBinary(): IJ.run(input_image_plus_copy, "Make Binary", "") # Run AnalyzeSkeleton analyze_skeleton = AnalyzeSkeleton_() analyze_skeleton.setup("", input_image_plus_copy) if prune_cycle_method == 'none': prune_index = analyze_skeleton.NONE elif prune_cycle_method == 'shortest_branch': prune_index = analyze_skeleton.SHORTEST_BRANCH elif prune_cycle_method == 'lowest_intensity_voxel': prune_index = analyze_skeleton.LOWEST_INTENSITY_VOXEL elif prune_cycle_method == 'lowest_intensity_branch': prune_index = analyze_skeleton.LOWEST_INTENSITY_BRANCH result = analyze_skeleton.run(prune_index, prune_ends, calculate_largest_shortest_path, input_image_plus_copy, True, True) # Save the results. save(result, output, show_detailed_info, calculate_largest_shortest_path) except Exception, e: