def gen_dcna(exports_config: Config.Config, study_config: Config.Config, verb):
# This is dCNA
# Requires cCNA to be generated already
helper.working_on(verb, message='Gathering files ...')
l_o_file = os.path.join(
study_config.config_map['output_folder'], 'data_{}.txt'.format(
constants.config2name_map['CONTINUOUS_COPY_NUMBER']))
c_o_file = os.path.join(
study_config.config_map['output_folder'], 'data_{}.txt'.format(
constants.config2name_map[exports_config.type_config]))
global thresholds
thresholds = [
float(x) for x in exports_config.config_map['thresholds'].split(',')
]
if os.path.exists(l_o_file):
helper.working_on(verb, message='Generating dCNA (CNA)...')
data = pd.read_csv(l_o_file, sep='\t')
cols = data.columns.values.tolist()[1:]
# This code here had an astonishing 5500x improvement compared to traversal over it as a 2D array, and yes 5500x
for c in cols:
data[c] = data[c].apply(lambda x: collapse(x))
data.to_csv(c_o_file, sep='\t', index=None)
else:
print(
'ERROR:: Cannot generate dCNA file because log2CNA file does not exist ...'
)
print(
'ERROR:: Either remove the DISCRETE data config file, or add a CONTINUOUS data config file '
)
helper.stars()
helper.stars()
exit(1)
def gen_log2cna(exports_config: Config.Config, study_config: Config.Config,
janus_path, verb):
# TODO janus_path argument is not used, can remove; replace verb with logger
helper.working_on(verb, message='Gathering files ...')
seg_file = os.path.join(
study_config.config_map['output_folder'],
'data_{}.txt'.format(constants.config2name_map['SEG']))
bed_file = exports_config.config_map['bed_file']
l_o_file = os.path.join(
study_config.config_map['output_folder'], 'data_{}.txt'.format(
constants.config2name_map[exports_config.type_config]))
helper.working_on(verb, message='Generating log2CNA...')
executable = 'Rscript'
r_script_path = os.path.join(os.dirname(__file__), R_SCRIPT_DIRNAME,
'seg2gene.r')
if os.path.exists(r_script_path):
cmd = ', '.join(
[executable, r_script_path, seg_file, bed_file, l_o_file])
logger.debug('Running R script command: ' + cmd)
rc = subprocess.call(cmd)
if rc != 0:
msg = "Non-zero exit code %i from R script command '%s'" % (rc,
cmd)
raise ValueError(msg)
else:
raise FileNotFoundError(
'Cannot find R script path {}'.format(r_script_path))
def main():
global meta_config
global study_config
global janus_path
global verb
helper.working_on(verb, message='Generating CNA files ...')
discrete_copy_number_data.gen_dcna(meta_config, study_config, verb)
helper.working_on(verb)
def main():
global meta_config
global study_config
global janus_path
global verb
helper.working_on(verb, message='Generating log2CNA files ...')
continuous_copy_number_data.gen_log2cna(meta_config, study_config,
janus_path, verb)
helper.working_on(verb)
def get_sample_ids(exports_config: Config.Config, verb) -> pd.Series:
data = pd.read_csv(os.path.join(exports_config.config_map['input_folder'],
exports_config.data_frame['FILE_NAME'][0]),
sep='\t',
usecols=['ID'])
helper.working_on(verb,
message='Parsing importable {} file ...'.format(
exports_config.type_config))
return data['ID'].drop_duplicates(keep='first', inplace=False)
def fix_hmmcopy_tsv(exports_config: Config.Config, study_config: Config.Config,
verb):
# Fix the header
# Gather ingredients
calls = []
output_folder = study_config.config_map['output_folder']
input_folder = exports_config.config_map['input_folder']
export_data = exports_config.data_frame
#input(export_data)
seg_temp = helper.get_temp_folder(output_folder, 'seg')
bed_filter = subprocess.check_output([
'awk "NR>1" {} | '
'awk -F"\\t" \'{{print $1}}\' | '
'uniq'.format(exports_config.config_map['bed_file'])
],
shell=True).decode("utf-8")
#input(bed_filter)
bed_filter = bed_filter.strip().split('\n')
bed_filter = bed_filter + ['chr' + a for a in bed_filter]
bed_filter = ['\\t' + a + '\\t' for a in bed_filter]
#input(bed_filter)
header = 'ID\\tchrom\\tloc.start\\tloc.end\\tnum.mark\\tseg.mean'
for i in range(len(export_data)):
input_file = os.path.join(input_folder, export_data['FILE_NAME'][i])
output_file = os.path.join(seg_temp, export_data['FILE_NAME'][i])
sample_id = export_data['SAMPLE_ID'][i]
helper.working_on(
verb, 'Refactoring cols: {}'.format(export_data['FILE_NAME'][i]))
output_temp = output_file + '.temp'
# Get all the genes in the .bed; save each line with a matching gene; rename the Sample_ID
# TODO get rid of this ugly & fragile bash script, rewrite using Python
# See comments by LEH in earlier commit
columns = '1' # placeholder for num.mark columns
cmd = 'echo "{}" > {}; '.format(header, output_temp) +\
'cat {} | '.format(input_file) +\
'awk \'BEGIN{{split("{}",t); '.format('|'.join(bed_filter))+\
'for (i in t) vals[t[i]]}} ($2 in vals)\' | '+\
'awk -F"\\t" \'{{ OFS="\\t"; '+\
'print "{}", $2, $3, $4, {}, $5}}\' >> {}; '.format(sample_id, output_temp, columns) +\
'mv {} {}'.format(output_temp, output_file)
calls.append(subprocess.Popen(cmd, shell=True))
exports_config.config_map['input_folder'] = seg_temp
exit_codes = [p.wait() for p in calls]
if any(exit_codes):
raise ValueError(
'ERROR:: Something went wrong when parsing HMMCopy format file? Please resolve the issue'
)
if verb:
print(exit_codes)
def verify_final_seg_file(exports_config: Config.Config, verb):
seg = open(
os.path.join(exports_config.config_map['input_folder'],
exports_config.data_frame['FILE_NAME'][0]), 'w')
header = seg.readline().strip().split('\t')
minimum_header = [
'ID', 'chrom', 'loc.start', 'loc.end', 'num.mark', 'seg.mean'
]
helper.working_on(verb, message='Asserting minimum header is in SEG file.')
if not all([a in header for a in minimum_header]):
print([a if a not in header else '' for a in minimum_header])
print(
'Missing headers from SEG file have been printed above, please ensure the data is not missing.'
)
exit(1)
def verify_final_discrete_file(exports_config: Config.Config, verb):
data = open(
os.path.join(exports_config.config_map['input_folder'],
exports_config.data_frame['FILE_NAME'][0]), 'w')
t_config = exports_config.type_config
header = data.readline().strip().split('\t')
minimum_header = ['Entrez_Gene_Id', 'Hugo_Symbol']
helper.working_on(
verb,
message='Asserting minimum header is in {} file.'.format(t_config))
if not any([a in header for a in minimum_header]):
print([a if a not in header else '' for a in minimum_header])
print(
'Missing header(s) from {} file have been printed above, ensure data isn\'t missing.'
.format(t_config))
exit(1)
def generate_expression_zscore(meta_config: Config.Config, input_file, outputPath, gepcomp, tcga, verb):
# Z-Scores written by Dr. L Heisler
helper.working_on(verb, message='Reading FPKM Matrix ...')
try:
raw_data = pd.read_csv(input_file, sep='\t')
except FileNotFoundError:
print('{} wrong file or file path'.format(input_file))
raise
helper.working_on(verb, message='Processing FPKM Matrix ...')
raw_scores = raw_data.drop(['Hugo_Symbol'], axis=1)
means = raw_scores.mean(axis=1)
sds = raw_scores.std(axis=1)
z_scores = ((raw_scores.transpose() - means) / sds).transpose()
z_scores = z_scores.fillna(0)
z_scores_data = z_scores.round(decimals=4)
z_scores_data = pd.concat([raw_data['Hugo_Symbol'], z_scores_data], axis=1)
helper.working_on(verb, message='Writing FPKM Z-Scores Matrix ...')
# Reformat the columns for comparison and TCGA data to keep only the columns used in the study mRNA expression continuous data
if gepcomp or tcga:
study_columns = []
for k in range(meta_config.data_frame.shape[0]):
study_columns.append(meta_config.data_frame['SAMPLE_ID'][k])
study_columns.insert(0,'Hugo_Symbol')
z_scores_data = z_scores_data[study_columns]
# Create the supplementary_data directory if it doesn't exist
if not os.path.exists(os.path.join(outputPath, 'supplementary_data')):
os.makedirs(os.path.join(outputPath, 'supplementary_data'), exist_ok=True)
if gepcomp:
# Output comparison Z scores
output_file_z_scores = os.path.join(outputPath, 'supplementary_data', 'data_{}_comparison.txt'.format(config2name_map[meta_config.alterationtype + ":" + 'Z-SCORE']))
z_scores_data.to_csv(output_file_z_scores, sep="\t", index=False)
# Delete all gepcomp files that are not in the supplementary folder
os.remove(input_file)
elif tcga:
# Output TCGA Z scores
output_file_z_scores = os.path.join(outputPath, 'supplementary_data', 'data_{}_tcga.txt'.format(config2name_map[meta_config.alterationtype + ":" + 'Z-SCORE']))
z_scores_data.to_csv(output_file_z_scores, sep="\t", index=False)
# Delete all TCGA files that are not in the supplementary folder
os.remove(input_file)
else:
# Output study Z scores
output_file_z_scores = os.path.join(outputPath, 'data_{}.txt'.format(config2name_map[meta_config.alterationtype + ":" + 'Z-SCORE']))
z_scores_data.to_csv(output_file_z_scores, sep="\t", index=False)
def main():
global meta_config
global study_config
global janus_path
global verb
helper.working_on(
verb,
message='Gathering and decompressing SEG files into temporary folder')
helper.decompress_to_temp(meta_config, study_config, verb)
helper.working_on(verb)
helper.working_on(
verb,
message=
'Fixing HMMCopy formatting, chromosome, and chromosome max-length ...')
fix_hmmcopy_tsv(meta_config, study_config, verb)
fix_chrom(meta_config, study_config, verb)
### fix_hmmcopy_max_chrom fixes the maximum chromosome length AND imputes the num.mark value
fix_hmmcopy_max_chrom(meta_config, study_config, janus_path, verb)
helper.working_on(verb)
helper.working_on(verb, message='Fixing .SEG IDs')
fix_seg_id(meta_config, study_config, verb)
helper.working_on(verb)
helper.working_on(verb, message='Concating SEG Files to export folder')
helper.concat_files(meta_config, study_config, verb)
helper.working_on(verb)
def main():
global meta_config
global study_config
global janus_path
global verb
helper.working_on(
verb,
message=
'Gathering and decompressing MRNA_EXPRESSION files into temporary folder'
)
helper.decompress_to_temp(meta_config, study_config, verb)
helper.working_on(verb)
helper.working_on(verb, message='Alpha sorting each file ...')
alpha_sort(meta_config, verb)
helper.working_on(verb)
helper.working_on(verb, message='Generating expression matrix ...')
generate_expression_matrix(meta_config, study_config, verb)
helper.working_on(verb)
# Works because shorting ...
if 'zscores' in meta_config.config_map.keys(
) and meta_config.config_map['zscores'].lower() == 'true':
helper.working_on(verb,
message='Generating expression Z-Score Meta ...')
meta.generate_meta_type(meta_config, study_config, verb)
#meta.generate_meta_type(meta_config.alterationtype + '_ZSCORES',
# {'profile_name': 'mRNA expression z-scores','profile_description': 'Expression level z-scores'}, study_config, verb)
helper.working_on(verb)
helper.working_on(verb,
message='Generating expression Z-Score Data ...')
generate_expression_zscore(meta_config, study_config, verb)
helper.working_on(verb)
def main():
global meta_config
global study_config
global janus_path
global logger
# imports are moved into the main (and only) method to work with the legacy component class
import logging
import os
from support import helper
from generate import meta
from generate.analysis_pipelines.MRNA_EXPRESSION.support_functions import alpha_sort, generate_expression_matrix, generate_expression_percentile, generate_expression_zscore, preProcRNA
from constants.constants import config2name_map
from utilities.constants import DATA_DIRNAME
verb = logger.isEnabledFor(logging.INFO) # TODO replace the 'verb' switch with logger
if meta_config.config_map.get('genelist'):
genelist = meta_config.config_map.get('genelist')
else:
genelist = os.path.join(os.path.dirname(__file__), DATA_DIRNAME, 'targeted_genelist.txt')
if meta_config.config_map.get('enscon'):
enscon = meta_config.config_map.get('enscon')
else:
enscon = os.path.join(os.path.dirname(__file__), DATA_DIRNAME, 'ensemble_conversion.txt')
logger.info('Started processing data for CAP_expression pipeline')
logger.info('Decompressing MRNA_EXPRESSION files to temporary folder')
meta_config = helper.relocate_inputs(meta_config, study_config, verb)
logger.info('Alpha sorting each file ...')
alpha_sort(meta_config, verb)
logger.info('Generating expression matrix ...')
generate_expression_matrix(meta_config, study_config, verb)
#preProcRNA - generate processed continuous data using the generated expression matrix - one for study and one for study comparison and one for TCGA data
preProcRNA(meta_config, study_config, '/data_{}_gepcomp.txt'.format(config2name_map[meta_config.alterationtype + ":" + meta_config.datahandler]), enscon, genelist, True, False)
preProcRNA(meta_config, study_config, '/data_{}.txt'.format(config2name_map[meta_config.alterationtype + ":" + meta_config.datahandler]), enscon, genelist, False, True)
if meta_config.config_map.get('zscores'):
# Generate the z-scores for mRNA expression data
logger.info('Generating expression Z-Score Data ...')
generate_expression_zscore(meta_config, os.path.join(study_config.config_map['output_folder'],
'data_{}.txt'.format(config2name_map[meta_config.alterationtype + ":" + meta_config.datahandler])),
study_config.config_map['output_folder']
, False, False, verb)
# Generate the mRNA expression percentile data
logger.info('Generating expression Percentile Data ...')
generate_expression_percentile(meta_config, os.path.join(study_config.config_map['output_folder'],
'data_{}.txt'.format(config2name_map[meta_config.alterationtype + ":" + 'Z-SCORE'])),
study_config.config_map['output_folder']
, False, False, verb)
# Generate the z-score sfor mRNA expression comparison data
logger.info('Generating expression Z-Score comparison Data ...')
generate_expression_zscore(meta_config, os.path.join(study_config.config_map['output_folder'],
'data_{}_gepcomp.txt'.format(config2name_map[meta_config.alterationtype + ":" + meta_config.datahandler])),
study_config.config_map['output_folder']
, True, False, verb)
# Generate the mRNA expression comparison percentile data
logger.info('Generating expression Percentile comparison Data ...')
generate_expression_percentile(meta_config, os.path.join(study_config.config_map['output_folder'], 'data_{}.txt'.format(config2name_map[meta_config.alterationtype + ":" + 'Z-SCORE'])),
study_config.config_map['output_folder']
, True, False, verb)
# Generate the z-scores for mRNA expression TCGA data
helper.working_on(verb, message='Generating expression TCGA Z-Score Data ...')
generate_expression_zscore(meta_config, os.path.join(study_config.config_map['output_folder'],
'data_{}_tcga.txt'.format(config2name_map[meta_config.alterationtype + ":" + meta_config.datahandler])),
study_config.config_map['output_folder']
, False, True, verb)
# Generate the TCGA mRNA expression percentile data
logger.info('Generating expression TCGA Percentile Data ...')
generate_expression_percentile(meta_config, os.path.join(study_config.config_map['output_folder'], 'supplementary_data',
'data_{}_tcga.txt'.format(config2name_map[meta_config.alterationtype + ":" + 'Z-SCORE'])),
study_config.config_map['output_folder']
, False, True, verb)
# Generate meta data within the handler and not in generator.py
# Generate metadata for mRNA expression continuous data
logger.info('Generating expression Meta ...')
meta.generate_meta_type(meta_config,study_config,logger)
# Generate metadata for mRNA expression z-score data
if meta_config.config_map.get('zscores'):
logger.info('Generating expression Z-Score Meta ...')
meta_config.datahandler = 'Z-SCORE'
meta.generate_meta_type(meta_config,study_config,logger)
logger.info('Finished processing data for CAP_expression pipeline')
def generate_expression_matrix(exports_config: Config.Config, study_config: Config.Config, verb):
# Output for data_expression_continuous_expression.txt data file
output_file = os.path.join(study_config.config_map['output_folder'],
'data_{}.txt'.format(config2name_map[exports_config.alterationtype + ":" + exports_config.datahandler]))
helper.working_on(verb, message='Reading FPKM data ...')
info: DataFrames = []
for i in range(exports_config.data_frame.shape[0]):
info.append(pd.read_csv(os.path.join(exports_config.config_map['input_folder'],
exports_config.data_frame['FILE_NAME'][i]),
sep='\t',
usecols=['gene_id','FPKM'])
.rename(columns={'FPKM': exports_config.data_frame['SAMPLE_ID'][i],
'gene_id': 'Hugo_Symbol'})
.drop_duplicates(subset='Hugo_Symbol', keep='last', inplace=False))
helper.working_on(verb, message='Merging all FPKM data ...')
if len(info) == 0:
raise ImportError('Attempting to import zero expression data, please remove expression data from study.')
elif len(info) == 1:
result = info[0]
else:
result = info[0]
for i in range(1, len(info)):
result: pd.DataFrame = pd.merge(result, info[i], how='outer', on='Hugo_Symbol')
result.drop_duplicates(subset='Hugo_Symbol', keep='last', inplace=True)
result.replace(np.nan, 0, inplace=True)
helper.working_on(verb, message='Writing all FPKM data ...')
result.to_csv(output_file, sep='\t', index=None)
# Append the gepcomp datafiles (if any)
gep_file = exports_config.config_map.get('gepfile')
if gep_file != None and os.path.exists(gep_file):
geplist = pd.read_csv(gep_file, sep=',')
geplist.columns = ['patient_id', 'file_name']
# Filter out the patient ID's that have already been included in the study
indices = []
for i in range(exports_config.data_frame.shape[0]):
for a, elem in enumerate(geplist.patient_id.tolist()):
if exports_config.data_frame['PATIENT_ID'][i] in elem:
indices.append(a)
geplist = geplist.drop(indices)
for index, row in geplist.iterrows():
info.append(pd.read_csv(row.file_name,
sep='\t',
usecols=['gene_id','FPKM'])
.rename(columns={'FPKM': row.patient_id,
'gene_id': 'Hugo_Symbol'})
.drop_duplicates(subset='Hugo_Symbol', keep='last', inplace=False))
helper.working_on(verb, message='Merging all FPKM data ...')
if len(info) == 0:
raise ImportError('Attempting to import zero expression data, please remove expression data from study.')
elif len(info) == 1:
result = info[0]
else:
result = info[0]
for i in range(1, len(info)):
result: pd.DataFrame = pd.merge(result, info[i], how='left', on='Hugo_Symbol')
result.drop_duplicates(subset='Hugo_Symbol', keep='last', inplace=True)
result.replace(np.nan, 0, inplace=True)
helper.working_on(verb, message='Writing all FPKM data ...')
# Output the gepcomp data
output_file_comp = os.path.join(study_config.config_map['output_folder'],
'data_{}_gepcomp.txt'.format(config2name_map[exports_config.alterationtype + ":" + exports_config.datahandler]))
result.to_csv(output_file_comp, sep='\t', index=None)
def main():
global meta_config
global study_config
global janus_path
global verb
helper.working_on(verb, message='Gathering and decompressing SEG files into temporary folder')
helper.decompress_to_temp(meta_config, study_config, verb)
helper.working_on(verb)
helper.working_on(verb, message='Fixing Chromosome numbering ...')
fix_chrom(meta_config, study_config, verb)
helper.working_on(verb)
helper.working_on(verb, message='Fixing .SEG IDs')
fix_seg_id(meta_config, study_config, verb)
helper.working_on(verb)
helper.working_on(verb, message='Concating SEG Files to export folder')
helper.concat_files(meta_config, study_config, verb)
helper.working_on(verb)
