box = DiffusionBox() # Rates are listed in uM, from table S1 # k_off and kcat rates are already in s^-1; k_on needs to be converted. rates = [ box.convert_membrane_rate(0.12), 3.0, box.convert_membrane_rate(0.11), 0.4, box.convert_membrane_rate(0.05), 0.1, 0.038, box.convert_membrane_rate(0.07), 1.0, 0.003, box.convert_cytosolic_rate(1.74), 0.2, 0.1, 0.01, 1.0, 0.01, ] rates = Rates(*rates) # We have used a intial Ras-GDP, and Ras-GAP concentrations of # 75 molecules/(um)2, 125 molecules/(um)3 respectively. ras = Ras(GDP=75) rasgap = RasGAP(125) rasgrp_final_vals, rasgrp_steady_states = [], [] rasgrp_counts = range(0, 200, 10)