def add_trajectory_info(df_stats, worm_index, timeseries_data, fps):
df_stats['worm_index'] = worm_index
df_stats['ini_time'] = timeseries_data['timestamp'].min() / fps
df_stats['tot_time'] = timeseries_data['timestamp'].size / fps
df_stats['frac_valid_skels'] = (~timeseries_data['length'].isnull()).mean()
is_fov_tosplit = was_fov_split(timeseries_data)
if is_fov_tosplit:
try:
assert len(set(timeseries_data['well_name']) - set(['n/a'])) == 1, \
"A single trajectory is spanning more than one well!"
except:
pdb.set_trace()
well_name = list(set(timeseries_data['well_name']) - set(['n/a']))[0]
df_stats['well_name'] = well_name
cols = df_stats.columns.tolist()
if not is_fov_tosplit:
cols = cols[-4:] + cols[:-4]
else: # there's one extra column
cols = cols[-5:] + cols[:-5]
#import pdb
#pdb.set_trace()
df_stats = df_stats[cols]
return df_stats
def tierpsy_trajectories_summary(
fname, time_windows, time_units, only_abs_ventral=False,
selected_feat=None, is_manual_index=False, delta_time=1/3):
"""
Calculate the trajectory summaries for a given file fname, within a given time window
(units of start time and end time are in frame numbers).
"""
fps = read_fps(fname)
data_in = read_data(fname, time_windows, time_units, fps, is_manual_index)
if data_in is None:
return [pd.DataFrame() for iwin in range(len(time_windows))]
timeseries_data, blob_features = data_in
is_fov_tosplit = was_fov_split(timeseries_data[0])
# is_fov_tosplit = False
if is_fov_tosplit:
fovsplitter = FOVMultiWellsSplitter(fname)
good_wells_df = fovsplitter.wells[['well_name','is_good_well']].copy()
# print(good_wells_df)
# initialize list of summaries for all time windows
all_summaries_list = []
# loop over time windows
for iwin,window in enumerate(time_windows):
if timeseries_data[iwin].empty:
all_summary = pd.DataFrame([])
else:
# initialize list of trajectory summaries for given time window
all_summary = []
# loop over worm indexes (individual trajectories)
for w_ind, w_ts_data in timeseries_data[iwin].groupby('worm_index'):
w_blobs = blob_features[iwin].loc[w_ts_data.index]
w_ts_data = w_ts_data.reset_index(drop=True)
w_blobs = w_blobs.reset_index(drop=True)
worm_feats = get_summary_stats(
w_ts_data, fps, w_blobs, delta_time,
only_abs_ventral=only_abs_ventral,
selected_feat=selected_feat
) # returns empty dataframe when w_ts_data is empty
worm_feats = pd.DataFrame(worm_feats).T
worm_feats = add_trajectory_info(worm_feats, w_ind, w_ts_data, fps)
all_summary.append(worm_feats)
# concatenate all trajectories in given time window into one dataframe
all_summary = pd.concat(all_summary, ignore_index=True, sort=False)
# attach whether the wells was good or bad
if is_fov_tosplit: # but only do this if we have wells
all_summary = all_summary.merge(good_wells_df,
on='well_name',
how='left')
# add dataframe to the list of summaries for all time windows
all_summaries_list.append(all_summary)
return all_summaries_list
def tierpsy_plate_summary(fname, time_windows, time_units, is_manual_index = False, delta_time = 1/3):
"""
Calculate the plate summaries for a given file fname, within a given time window
(units of start time and end time are in frame numbers).
"""
fps = read_fps(fname)
data_in = read_data(fname, time_windows, time_units, fps, is_manual_index)
# if manual annotation was chosen and the trajectories_data does not contain
# worm_index_manual, then data_in is None
# if time_windows in seconds and fps is not defined (fps=-1), then data_in is None
if data_in is None:
return [pd.DataFrame() for iwin in range(len(time_windows))]
timeseries_data, blob_features = data_in
# was the fov split in wells? only use the first window to detect that,
# and to extract the list of well names
is_fov_tosplit = was_fov_split(timeseries_data[0])
# is_fov_tosplit = False
# initialize list of plate summaries for all time windows
plate_feats_list = []
for iwin,window in enumerate(time_windows):
if is_fov_tosplit == False:
plate_feats = get_summary_stats(timeseries_data[iwin], fps, blob_features[iwin], delta_time)
plate_feats_list.append(pd.DataFrame(plate_feats).T)
else:
# get list of well names in this time window
# (maybe some wells looked empty during a whole window,
# this prevents errors later on)
well_names_list = list(set(timeseries_data[iwin]['well_name']) - set(['n/a']))
# create a list of well-specific, one-line long dataframes
well_feats_list = []
for well_name in well_names_list:
# find entries in timeseries_data[iwin] belonging to the right well
idx_well = timeseries_data[iwin]['well_name'] == well_name
well_feats = get_summary_stats(timeseries_data[iwin][idx_well].reset_index(),
fps,
blob_features[iwin][idx_well].reset_index(),
delta_time)
# first prepend the well_name_s to the well_feats series,
# then transpose it so it is a single-row dataframe,
# and append it to the well_feats_list
well_name_s = pd.Series({'well_name':well_name})
well_feats_list.append(pd.DataFrame(pd.concat([well_name_s,well_feats])).T)
# check: did we find any well?
if len(well_feats_list) == 0:
plate_feats_list.append(pd.DataFrame())
else:
# now concatenate all the single-row df in well_feats_list in a single df
# and append it to the growing list (1 entry = 1 window)
plate_feats = pd.concat(well_feats_list, ignore_index=True, sort=False)
plate_feats_list.append(plate_feats)
return plate_feats_list
def save_feats_stats(features_file, derivate_delta_time):
with pd.HDFStore(features_file, 'r') as fid:
fps = fid.get_storer('/trajectories_data').attrs['fps']
timeseries_data = fid['/timeseries_data']
blob_features = fid[
'/blob_features'] if '/blob_features' in fid else None
is_fov_tosplit = was_fov_split(
features_file) # do we need split-FOV sumaries?
# check
if is_fov_tosplit:
assert 'well_name' in timeseries_data.columns, (
'fov_wells in features file but no well_name in timeseries_data')
#Now I want to calculate the stats of the video
if is_fov_tosplit:
# get summary stats per well and then concatenate them all
well_name_list = list(set(timeseries_data['well_name']) - set(['n/a']))
exp_feats = []
for wc, well in enumerate(well_name_list):
print('Processing well {} out of {}'.format(
wc, len(well_name_list)))
idx = timeseries_data['well_name'] == well
# calculate stats per well
tmp = get_summary_stats(timeseries_data[idx].reset_index(), fps,
blob_features[idx].reset_index(),
derivate_delta_time)
tmp = pd.DataFrame(zip(tmp.index, tmp), columns=['name', 'value'])
tmp['well_name'] = well
exp_feats.append(tmp)
# now concat all
exp_feats = pd.concat(exp_feats, ignore_index=True)
else: # we don't need to split the FOV
exp_feats = get_summary_stats(timeseries_data, fps, blob_features,
derivate_delta_time)
# save on disk
# now if is_fov_tosplit exp_feats is a dataframe, otherwise a series
if len(exp_feats) > 0:
# different syntax according to df or series
if is_fov_tosplit:
tot = max(len(x) for x in exp_feats['name'])
dtypes = {
'name': 'S{}'.format(tot),
'value': np.float32,
'well_name': 'S3'
}
exp_feats_rec = exp_feats.to_records(index=False,
column_dtypes=dtypes)
else:
tot = max(len(x) for x in exp_feats.index)
dtypes = [('name', 'S{}'.format(tot)), ('value', np.float32)]
exp_feats_rec = np.array(list(zip(exp_feats.index, exp_feats)),
dtype=dtypes)
# write on hdf5 file
with tables.File(features_file, 'r+') as fid:
for gg in ['/features_stats']:
if gg in fid:
fid.remove_node(gg)
fid.create_table('/',
'features_stats',
obj=exp_feats_rec,
filters=TABLE_FILTERS)
