def structure_score(dom, pep, models, model_seqs, mat):
'''
'''
model_max = {"1SSH": 49.900, "1ZUK": 61.800, "1N5Z": 48.100,
"2KYM": 47.100, "2RQW": 56.800, "2VKN": 51.100}
model_scr = []
for model in model_seqs:
dom_align = nw.global_align(dom, "".join(model_seqs[model]["D"]),
gap_open=-100, gap_extend=-1,
matrix=mat)
dom_matches = contact_map_helper(dom_align)
pep_align = nw.global_align(pep, "".join(model_seqs[model]["P"]),
gap_open=-100, gap_extend=-1,
matrix=mat)
pep_matches = contact_map_helper(pep_align)
score = 0
count = 0
# max_model = sum(models[model].values())
for i in models[model]:
if i[0] in dom_matches and i[1] in pep_matches:
score += models[model][i]
count += 1
if count == 0:
model_scr.append(0)
else:
model_scr.append((score / float(model_max[model])) / count)
return max(model_scr)
def worker(seq1, seq2): # ignore old / previously blased ones. # start = time.clock() align1 = nwalign.global_align(seq1, seq2, matrix='deps/nuc44') align2 = nwalign.global_align(seq1, rev_compliment(seq2), gap_open=-1, gap_extend=-1, matrix='deps/nuc44') t1 = sim(align1[0], align1[1]) t2 = sim(align2[0], align2[1]) res = float(max(t1, t2)) / float(min(len(seq1), len(seq2))) return res
def score_phonetic_alignment(srcw,
tgtw,
slang,
tlang,
sim_matrix_path,
gap_start_p=-1.0,
gap_extend_p=-1.0):
# convert to ascii required by align library
nsrcw = ''.join(
make_ascii(srcw, slang) if slang in
langinfo.SCRIPT_RANGES else [str(c) for c in srcw])
ntgtw = ''.join(
make_ascii(tgtw, tlang) if tlang in
langinfo.SCRIPT_RANGES else [str(c) for c in tgtw])
## use global alignment
src_aln, tgt_aln = nw.global_align(nsrcw,
ntgtw,
matrix=sim_matrix_path,
gap_open=gap_start_p,
gap_extend=gap_extend_p)
return nw.score_alignment(src_aln,
tgt_aln,
matrix=sim_matrix_path,
gap_open=gap_start_p,
gap_extend=gap_extend_p)
def sound_seq_distance_str(self,seq1_str, seq2_str):
seq1_str = np.asanyarray(seq1_str)
seq2_str = np.asanyarray(seq2_str)
align = nw.global_align(seq1_str.tostring(), seq2_str.tostring(), gap_open=0, gap_extend=-5, matrix='/tmp/som.costs')
len1 = len(seq1_str.tostring())
len2 = len(seq2_str.tostring())
return (-nw.score_alignment(*align, gap_open=0, gap_extend=-5, matrix='/tmp/som.costs'))/(len1+len2+0.0)
def score_phonetic_alignment(srcw,tgtw,slang,tlang,sim_matrix_path,gap_start_p=-1.0,gap_extend_p=-1.0):
# convert to ascii required by align library
nsrcw=''.join(make_ascii(srcw,slang) if slang in langinfo.SCRIPT_RANGES else [str(c) for c in srcw ])
ntgtw=''.join(make_ascii(tgtw,tlang) if tlang in langinfo.SCRIPT_RANGES else [str(c) for c in tgtw ])
## use global alignment
src_aln,tgt_aln=nw.global_align(nsrcw,ntgtw,matrix=sim_matrix_path, gap_open=gap_start_p, gap_extend=gap_extend_p)
return nw.score_alignment(src_aln,tgt_aln,matrix=sim_matrix_path, gap_open=gap_start_p, gap_extend=gap_extend_p)
def _align(self):
matrix = self._get_matrix_file(match=self._match,
mismatch=self._mismatch,
matrix=self._matrix)
aln = nw.global_align(self.query.sequence,
self.target.sequence,
gap_open=self._gap_open,
gap_extend=self._gap_extend,
matrix=matrix)
return aln
def _align(self):
matrix = self._get_matrix_file(match=self._match,
mismatch=self._mismatch,
matrix=self._matrix)
aln = nw.global_align(self.query.sequence,
self.target.sequence,
gap_open=self._gap_open,
gap_extend=self._gap_extend,
matrix=matrix)
return aln
def alignMotifs(junctionSeqStart, junctionSeqEnd):
"""Align the sequence of two motifs."""
for side in ['side1', 'side2']:
if len(junctionSeqStart.loc[side]) != len(junctionSeqEnd.loc[side]):
s1 = junctionSeqStart.loc[side]
s2 = junctionSeqEnd.loc[side]
a, b = nw.global_align(s1, s2, gap_open=-10)
#print side, a, b
junctionSeqStart.loc[side] = a.replace('-', '_')
junctionSeqEnd.loc[side] = b.replace('-', '_')
return junctionSeqStart, junctionSeqEnd
def calc_distance(x, y):
if aln_fn == '':
x, y = nw.global_align(x, y, gap_open=-5, gap_extend=-1, match=1)
dxy = 0
lxy = 0
for i in range(min(len(x), len(y))):
if x[i] != '-' and y[i] != '-':
lxy += 1
if x[i] != y[i]:
dxy += 1
return 1. * dxy / lxy
def alignMotifs(junctionSeqStart, junctionSeqEnd):
"""Align the sequence of two motifs."""
for side in ['side1', 'side2']:
if len(junctionSeqStart.loc[side]) != len(junctionSeqEnd.loc[side]):
s1 = junctionSeqStart.loc[side]
s2 = junctionSeqEnd.loc[side]
a, b = nw.global_align(s1, s2, gap_open=-10)
#print side, a, b
junctionSeqStart.loc[side] = a.replace('-', '_')
junctionSeqEnd.loc[side] = b.replace('-', '_')
return junctionSeqStart, junctionSeqEnd
def calc_distance(x,y):
if aln_fn == '':
x, y = nw.global_align(x, y, gap_open=-5, gap_extend=-1, match=1)
dxy = 0
lxy = 0
for i in range(min(len(x),len(y))):
if x[i] != '-' and y[i] != '-':
lxy += 1
if x[i] != y[i]:
dxy += 1
return 1.*dxy/lxy
def getPathsFromSequence(seqInit, seqEnd):
a, b = nw.global_align(seqInit, seqEnd, gap_open=-10)
# add bases to vecInit
while len(vecInit) < len(vecEnd):
vecInit += '_'
while len(vecInit) > len(vecEnd):
vecEnd += '_'
steps = getSteps(vecInit, vecEnd, numSteps)
graph = getGraphFromSteps(steps)
paths = np.array(list(dfs_paths(graph, vecInit, vecEnd)))
paths_parsed = formatPaths(paths, numSteps / 2)
def getPathsFromSequence(seqInit, seqEnd):
a, b = nw.global_align(seqInit, seqEnd, gap_open=-10)
# add bases to vecInit
while len(vecInit) < len(vecEnd):
vecInit += '_'
while len(vecInit) > len(vecEnd):
vecEnd += '_'
steps = getSteps(vecInit, vecEnd, numSteps)
graph = getGraphFromSteps(steps)
paths = np.array(list(dfs_paths(graph, vecInit, vecEnd)))
paths_parsed = formatPaths(paths, numSteps/2)
def testNeedleman(N):
alpha = [
'A', 'B', 'C', 'D', 'E', 'F', 'G', 'H', 'I', 'J', 'K', 'L', 'M', 'N',
'O', 'P', 'Q', 'R', 'S', 'T', 'U', 'V', 'W', 'X', 'Y', 'Z'
]
Validated = 0
SamePath = 0
for epoch in tqdm(range(N)):
sizeA = random.randint(1, SIZEMAX)
sizeB = random.randint(sizeA // 2, 2 * sizeA)
A = ""
B = ""
for i in range(sizeA):
A += alpha[random.randint(0, len(alpha) - 1)]
for i in range(sizeB):
B += alpha[random.randint(0, len(alpha) - 1)]
aligned = nw.global_align(A,
B,
matrix='atiam-fpa_alpha.dist',
gap_open=-3,
gap_extend=-3)
score = nw.score_alignment(aligned[0],
aligned[1],
gap_open=-3,
gap_extend=-3,
matrix='atiam-fpa_alpha.dist')
res = (aligned[0], aligned[1], score)
try:
(a, b, s) = myNeedleman(A,
B,
matrix='atiam-fpa_alpha.dist',
gap_open=-3,
gap_extend=-3)
if s == score:
Validated += 1
if res == (a, b, s):
SamePath += 1
except RuntimeError:
print(A, B)
pass
print(str(100 * Validated / N) + "% are validated.")
print(str(100 * SamePath / N) + "% have the exact same path.")
def sim(a, b):
# This data _has_ been aligned, but sometimes they're not the same length?
# If that's the case, send it through NW
if len(a) != len(b):
(a, b) = nw.global_align(a,b)
print "Length mismatch, realigned using nw"
s = 0
n = 0
for i in range(len(a)):
if(a[i] == '-' and b[i] == '-'):
continue
if(a[i] == b[i]):
s += 1
n += 1
return float(s)/float(n)
def merge_overlaps(seq1, qual1, seq2, qual2):
"""Merge two sequences that overlap at the ends.
This assumes both sequences are given in forward orientation. The second
sequence is first complemented, then the two sequences are aligned to
find the coordinates of overlap. The base calls are then compared for
each position in the overlap; if there is a mismatch, compare the quality
scores (reversed for seq2) using the ASCII values and keep the best one.
>>> ord('B')
66
The alignment is done using the nwalign package which implements the
Needleman-Wunsch algorithm in C via Cython.
"""
# reverse-complement the second sequence
seq1 = seq1.upper() # to make sure
seq2 = quick_revcom(seq2)
qual2 = qual2[::-1]
# run the alignment
alignment = nw.global_align(seq1, seq2, gap_open=-50, gap_extend=-2)
seqln1 = alignment[0]
seqln2 = alignment[1]
# compose the merged sequence
merge_list = []
pos = 0
qpos1 = 0
qpos2 = 0
while pos < len(alignment[0]):
if seqln1[pos] is '-' or seqln1[pos] is 'N':
merge_list.append(seqln2[pos])
qpos1 +=1
elif seqln2[pos] is '-' or seqln2[pos] is 'N':
merge_list.append(seqln1[pos])
qpos2 +=1
elif seqln1[pos] is seqln2[pos]:
merge_list.append(seqln1[pos])
else: # determine the consensus of the overlap using quality scores
if ord(qual1[qpos1]) >= ord(qual2[qpos2]):
merge_list.append(seqln1[pos])
else:
merge_list.append(seqln2[pos])
pos +=1
qpos1 +=1
qpos2 +=1
merged_seq = ''.join(merge_list)
return merged_seq
def align_transliterations(src_wordlist, tgt_wordlist, lang):
for srcw, tgtw in itertools.izip(src_wordlist, tgt_wordlist):
# convert to ascii required by align library
nsrcw = ''.join(make_ascii(srcw, lang))
ntgtw = ''.join(make_ascii(tgtw, lang))
# use global alignment
src_aln, tgt_aln = nw.global_align(nsrcw, ntgtw)
# make it readable again
src_aln = restore_from_ascii(src_aln, lang)
tgt_aln = restore_from_ascii(tgt_aln, lang)
yield (src_aln, tgt_aln)
def gs_align(left, right):
lstring, lduration, lstart, lend = left
rstring, rduration, rstart, rend = right
lalign, ralign = nw.global_align(lstring, rstring)
def insert_placeholders(align, duration, start, end):
for i, ch in enumerate(align):
if ch == '-':
duration.insert(i, None)
start.insert(i, None)
end.insert(i, None)
insert_placeholders(lalign, lduration, lstart, lend)
insert_placeholders(ralign, rduration, rstart, rend)
align_ix = [i for i in range(len(lalign)) if lalign[i] == ralign[i]]
return lalign, ralign, align_ix
def align_transliterations(src_wordlist,tgt_wordlist,lang):
for srcw,tgtw in itertools.izip(src_wordlist,tgt_wordlist):
# convert to ascii required by align library
nsrcw=''.join(make_ascii(srcw,lang))
ntgtw=''.join(make_ascii(tgtw,lang))
# use global alignment
src_aln,tgt_aln=nw.global_align(nsrcw,ntgtw)
# make it readable again
src_aln=restore_from_ascii(src_aln,lang)
tgt_aln=restore_from_ascii(tgt_aln,lang)
yield (src_aln,tgt_aln)
def gs_align(left, right):
lstring, lduration, lstart, lend = left
rstring, rduration, rstart, rend = right
lalign, ralign = nw.global_align(lstring, rstring)
def insert_placeholders(align, duration, start, end):
for i, ch in enumerate(align):
if ch == '-':
duration.insert(i, None)
start.insert(i, None)
end.insert(i, None)
insert_placeholders(lalign, lduration, lstart, lend)
insert_placeholders(ralign, rduration, rstart, rend)
align_ix = [i for i in range(len(lalign)) if lalign[i] == ralign[i]]
return lalign, ralign, align_ix
def cl2string(recorded, target):
all_labels = list(set([l for _, l in recorded] + [l for _, l in target]))
l2char = {all_labels[i]: string.ascii_letters[i]
for i in range(len(all_labels))}
rec_s = "".join(l2char[l] for _, l in recorded)
tgt_s = "".join(l2char[l] for _, l in target)
recalign, tgtalign = nw.global_align(rec_s, tgt_s)
rectimes = [t for t, _ in recorded]
tgttimes = [t for t, _ in target]
for i, (recchar, tgtchar) in enumerate(zip(recalign, tgtalign)):
if tgtchar == '-':
tgttimes.insert(i, None)
if recchar == '-':
rectimes.insert(i, None)
return recalign, tgtalign, rectimes, tgttimes
def cl2string(recorded, target):
all_labels = list(set([l for _, l in recorded] + [l for _, l in target]))
l2char = {
all_labels[i]: string.ascii_letters[i]
for i in range(len(all_labels))
}
rec_s = "".join(l2char[l] for _, l in recorded)
tgt_s = "".join(l2char[l] for _, l in target)
recalign, tgtalign = nw.global_align(rec_s, tgt_s)
rectimes = [t for t, _ in recorded]
tgttimes = [t for t, _ in target]
for i, (recchar, tgtchar) in enumerate(zip(recalign, tgtalign)):
if tgtchar == '-':
tgttimes.insert(i, None)
if recchar == '-':
rectimes.insert(i, None)
return recalign, tgtalign, rectimes, tgttimes
def align():
hg19 = Fasta('hg19.fa')
print hg19.keys()
hg19Chr = sorted(hg19.keys(), reverse=True)
YRI = Fasta('YRIref.fasta')
print YRI.keys()
YRIChr = sorted(YRI.keys())
print hg19[hg19Chr[0]][:20]
print YRI[YRIChr[0]][:20]
print hg19[hg19Chr[0]][:20]
print YRI[YRIChr[0]][:20]
fhout = open('hg19_YRI_diff.bed', 'w')
header = 'chrom, chromStart, chromEnd, hg19, YRI \n'
fhout.write(header)
for each in hg19Chr:
seq1 = hg19[each][:10000]
seq2 = YRI[each][:10000]
print 'reached 1'
print 'doing alignment for ', each
alignment = nw.global_align(seq1, seq2, gap=-2, matrix=None, match=1, mismatch=-1)
print 'reached 2'
len1 = len(alignment[0]) #hg19
len2 = len(alignment[1]) #YRI
if len2>len1:
x = len2
else:
x = len1
for i in range(x):
if alignment[0][i] != alignment[1][i]:
#write to fhout
outline = each + ',' + str(i) + ',' + str(i+1) + ',' + alignment[0][i] + ',' + alignment[1][i] + '\n'
fhout.write(outline)
fhout.close()
def pairwise_from_seq(self, in_seq):
if self.pairwise is None:
self.calculate_pairwise()
s_seq, i_seq = global_align(self.seq, in_seq)
scount = -1
mcount = -1
i_inds = []
for s, i in zip(s_seq, i_seq):
if s != '-':
scount += 1
if i != '-':
mcount += 1
if scount != -1 and mcount != -1:
i_inds.append((mcount, scount))
pdict = {}
for s1, s2 in product(i_inds, repeat = 2):
if s1[1] != s2[1]:
pdict[(s1[0], s2[0])] = self.pairwise[(s1[1], s2[1])]
return pdict
def alignSequences(targetsite_sequence, window_sequence, max_errors=7):
window_sequence = window_sequence.upper()
# Try both strands
query_regex_standard, query_regex_gap = regexFromSequence(
targetsite_sequence, errors=max_errors)
alignments = list()
alignments.append(('+', 'standard',
regex.search(query_regex_standard, window_sequence,
regex.BESTMATCH)))
alignments.append(('-', 'standard',
regex.search(query_regex_standard,
reverseComplement(window_sequence),
regex.BESTMATCH)))
alignments.append(('+', 'gapped',
regex.search(query_regex_gap, window_sequence,
regex.BESTMATCH)))
alignments.append(('-', 'gapped',
regex.search(query_regex_gap,
reverseComplement(window_sequence),
regex.BESTMATCH)))
lowest_distance_score = 100
chosen_alignment = None
chosen_alignment_strand = None
for i, aln in enumerate(alignments):
strand, alignment_type, match = aln
if match != None:
substitutions, insertions, deletions = match.fuzzy_counts
distance_score = substitutions + (insertions + deletions) * 3
if distance_score < lowest_distance_score:
chosen_alignment = match
chosen_alignment_strand = strand
lowest_distance_score = distance_score
if chosen_alignment:
match_sequence = chosen_alignment.group()
match_substitutions, match_insertions, match_deletions = chosen_alignment.fuzzy_counts
distance = sum(chosen_alignment.fuzzy_counts)
length = len(match_sequence)
start = chosen_alignment.start()
end = chosen_alignment.end()
path = os.path.dirname(os.path.abspath(__file__))
if match_insertions or match_deletions:
realigned_match_sequence, realigned_target = nw.global_align(
match_sequence,
targetsite_sequence,
gap_open=-10,
gap_extend=-100,
matrix='{0}/NUC_SIMPLE'.format(path))
return [
realigned_match_sequence, distance, length,
chosen_alignment_strand, start, end, realigned_target
]
else:
return [
match_sequence, distance, length, chosen_alignment_strand,
start, end, targetsite_sequence
]
else:
return [''] * 6 + ['none']
def align_sequences(self, seq1, seq2):
result = nw.global_align(seq1, seq2, matrix='BLOSUM62.txt')
return result
kw[k] = int(kwargs[2 * i + 1])
return a, b, kw
while True:
client, address = server.accept()
data = True
while data:
try:
data = client.recv(CHUNK).strip()
if data == "EXIT":
client.close()
server.close()
print "EXITING service"
sys.exit(0)
a, b, kwargs = get_args(data)
r = global_align(a, b, **kwargs)
client.send(" ".join(r))
except Exception, e:
try:
client.send("ERROR:" + str(e))
except socket.error:
# they already closed...
client.close()
break
client.close()
atexit.register(server.close)
while True:
client, address = server.accept()
data = True
while data:
try:
data = client.recv(CHUNK).strip()
if data == "EXIT":
client.close()
server.close()
print "EXITING service"
sys.exit(0)
a,b, kwargs = get_args(data)
r = global_align(a, b, **kwargs)
client.send(" ".join(r))
except Exception, e:
try:
client.send("ERROR:" + str(e))
except socket.error:
# they already closed...
client.close()
break
client.close()
atexit.register(server.close)
def compare_datapoints(p1, p2):
gist_1 = p1[2:len(p1) - 2]
gist_2 = p2[2:len(p2) - 2]
gist_1_flipped = list(gist_1)
# We also try the 'flipped' (mirrored) version of the gesture to allow comparison between left-handed and right-handed gestures
# (although it's not super accurate).
# Need to take into account not to flip spine (so we drop the last 3 parts)
for i in range(0, (len(gist_1_flipped) - 3) / 2):
tmp = GestureComparison._flip_quadrants(gist_1_flipped[i])
# print gist_1_flipped[i] + " --> " + tmp
# print str(i) + "<->" + str(len(gist_1_flipped) / 2 + i - 3 + 1)
gist_1_flipped[i] = GestureComparison._flip_quadrants(
gist_1_flipped[len(gist_1_flipped) / 2 + i - 3 + 1])
gist_1_flipped[len(gist_1_flipped) / 2 + i - 3 + 1] = tmp
score = 0.0
score_flipped = 0.0
for i in range(0, len(gist_1)):
if gist_1[i] != 0 and gist_2[i] != '':
res = nwalign.global_align(
gist_1[i],
gist_2[i],
matrix=os.path.dirname(os.path.realpath(__file__)) +
'/alignment.matrix')
this_score = nwalign.score_alignment(
res[0],
res[1],
gap_open=0,
gap_extend=-5,
matrix=os.path.dirname(os.path.realpath(__file__)) +
'/alignment.matrix')
if i >= len(gist_1) - 3:
this_score *= 2
score += this_score
res = nwalign.global_align(
gist_1_flipped[i],
gist_2[i],
matrix=os.path.dirname(os.path.realpath(__file__)) +
'/alignment.matrix')
this_score_flipped = nwalign.score_alignment(
res[0],
res[1],
gap_open=0,
gap_extend=-5,
matrix=os.path.dirname(os.path.realpath(__file__)) +
'/alignment.matrix')
if i >= len(gist_1) - 3:
this_score_flipped *= 2
score_flipped += this_score_flipped
# print str(score_flipped) + " " + str(score)
if score_flipped > score:
score_flipped -= abs(int(p1[-2]) -
int(p2[-1])) + abs(int(p1[-1]) - int(p2[-2]))
# print "using flipped! " + p1[1] + " " + p2[1] + " " + str(score_flipped) + " > " + str(score)
return -score_flipped
else:
score -= abs(int(p1[-2]) -
int(p2[-2])) + abs(int(p1[-1]) - int(p2[-1]))
return -score
singreps=line.split("#")
rep_count.append(len(singreps)-1)
poslist=[]
for singrep in singreps:
if singrep != "\n":
repinfo=singrep.split(":")
poslist.append(int(repinfo[1]))
rep_pos.append(poslist)
locus_num.append(i)
locus.append(curloc)
fullscore=0
array=()
score=0
for k in range(len(locus)):
print "Processing repeat", k, "of", locus[k]
fullscore=nw.score_alignment(rep[k], rep[k], gap_open=-5,\
gap_extend=-2, matrix='/home/CT/server/pybin/BLOSUM62')
print >> fileout, ">", k, locus[k], rep[k], rep_count[k], rep_pos[k]
for j in range(len(rep)):
array=nw.global_align(rep[k], rep[j], gap_open=-5,\
gap_extend=-2, matrix='/home/CT/server/pybin/BLOSUM62')
score=nw.score_alignment(array[0], array[1], gap_open=-5,\
gap_extend=-2, matrix='/home/CT/server/pybin/BLOSUM62')
if score>0 and score/float(fullscore)>=threshold and j!=k:
print >> fileout, j, locus[j], rep[j], rep_count[j], rep_pos[j]
filein.close()
fileout.close()
def run_align_nw(data):
for s in data:
nw.global_align(s[0], s[1])
0:MAX_SEQUENCES] # only do the first 50 for speed..
similarity_matrix = np.zeros((len(unique_sequences), len(unique_sequences)))
dist_matrix = np.zeros((len(unique_sequences), len(unique_sequences)))
scoring = sw.ScoringMatrix('scoring_matrix.txt')
sw = sw.LocalAlignment(scoring)
match = 2
n = 0
for x, seq1 in enumerate(unique_sequences):
for y, seq2 in enumerate(unique_sequences):
alignment = nw.global_align(allsequences[seq1], allsequences[seq2])
score = float(
nw.score_alignment(alignment[0],
alignment[1],
gap_open=-5,
gap_extend=-2,
matrix='scoring_matrix.txt'))
n = float(len(alignment[0]) * match)
if abs(score) > n:
score = 0
similarity_matrix[x, y] = int(score)
dist_matrix[x, y] = float(score / n)
'''Lee un fasta y devuelve la secuencia que contiene'''
F = open(fastafile)
#Definimos la variable "secuencia"
secuencia = ''
#Iniciamos el bucle de lectura
for linea in F:
#Asociamos a la variable "linea" cada línea del archivo, pero sin el salto de línea
linea = linea.strip('\n')
#Comprobamos que la línea no está vacía, ni empieza por '>' (1ª linea de info)
if linea != '' and linea[0] != '>' :
#Añadimos a la variable "secuencia" cada iteración de linea
secuencia = secuencia + linea
return secuencia
D = fasta2sec('Q9V429.fasta')
H = fasta2sec('P10599.fasta')
acounter = 0
bcounter = 0
a, b = nw.global_align(D, H, matrix='BLOSUM62.txt')
for aa in a:
if aa != '-':
acounter += 1
for aa in b:
if aa != '-':
bcounter += 1
#print a
#print b
print ((acounter*100./len(a))+(bcounter*100./len(b))) / 2
matrix='atiam-fpa_alpha.dist',
gap_open=-5,
gap_extend=-2)
# Reference code for testing
import nwalign as nw
print("myNeedleman")
print(
myNeedleman("CEELECANTH",
"PELICAN",
matrix='atiam-fpa_alpha.dist',
gap_open=-1,
gap_extend=-1))
print("Nwalign")
aligned = nw.global_align("CEELECANTH",
"PELICAN",
matrix='atiam-fpa_alpha.dist')
score = nw.score_alignment(aligned[0],
aligned[1],
gap_open=-1,
gap_extend=-1,
matrix='atiam-fpa_alpha.dist')
print('Results for basic gap costs (linear)')
print(aligned[0])
print(aligned[1])
print('Score : ' + str(score))
print("myNeedleman")
print(
myNeedleman("CEELECANTH",
"PELICAN",
def _RealignRead(self, read):
has_score = False
if read.is_unmapped is True:
#self._out.write(read)
return read
tags = read.tags
'''If any of the read tags are AS, then remember the read has an
existing score.'''
for i in range(0, len(tags)):
if tags[i][0] == 'AS':
has_score = True
continue
if self.only_gapped is True:
has_indel = False
for c in read.cigar:
if c[0] == 1 or c[0] == 2:
# read has an indel
has_indel = True
break
if has_indel == False:
# Read must not have an indel
'''
If the read is a perfect match then don't realign
'''
print(read.qname + ', ' + str(read.cigar) + " does not have an indel")
#self._out.write(read)
return read
fivep_soft_clip = 0
threep_soft_clip = 0
cigar_last = len(read.cigar) - 1
if read.cigar[0][0] == 4:
fivep_soft_clip = read.cigar[0][1]
if read.cigar[cigar_last][0] == 4:
threep_soft_clip = read.cigar[cigar_last][1]
ref = self.ref.fetch(reference=self.refnames[read.tid],
start=read.aend - read.alen - fivep_soft_clip,
end=read.aend + threep_soft_clip)
# Realign sense strand reads
query = ''
subject = ''
if self.reverse_sense is True and read.is_reverse is False:
query = self.ReverseSeq(read.seq)
subject = self.ReverseSeq(ref.upper())
#query = Seq(read.seq).complement().tostring()
#subject = Seq(ref.upper()).complement().tostring()
else:
query = read.seq
subject = ref.upper()
print query, subject
aln = nw.global_align(query, subject,
gap_open=self.gap_open,
gap_extend=self.gap_extend,
matrix=self.matrix)
if self.compute_scores is True:
score = nw.score_alignment(aln[0], aln[1],
gap_open=self.gap_open,
gap_extend=self.gap_extend,
matrix=self.matrix)
if has_score is True:
as_index = None
tags = read.tags
for i in range(0, len(tags)):
if tags[i][0] == 'AS':
as_index = i
if as_index is None:
raise ValueError("Read " + read.qname +
" is missing an alignment score.")
tags[as_index] = ('AS', score)
read.tags = tags
else:
read.tags = [('AS', score)] + read.tags
bam_cigar = self._MakeBamCigar(aln, read)
if self.reverse_sense is True and read.is_reverse is False:
bam_cigar.reverse()
if self.verbose is True:
self.PrettyPrint(read, aln, bam_cigar)
# New read
read.cigar = bam_cigar
read.tags = read.tags + [('OC', self._MakeSamCigar(read.cigar)),
('OP', read.pos)]
#self._out.write(read)
return read
type = str,
help = "Which allele to align against"
)
if __name__ == '__main__':
args = parser.parse_args()
print args
with open(args.input_filename,'r') as f:
d = parse_fasta(f.read())
allele = args.reference_allele
if allele in d:
refseq = d[allele]
else:
refseq = d[allele.replace("*", "")]
result = {}
for k,v in d.iteritems():
x, y = nw.global_align(refseq, v, gap_open=-40, gap_extend=-20, matrix='BLOSUM50')
good_positions = [i for i,xi in enumerate(x) if xi != "-"]
x_subset = "".join(x[i] for i in good_positions)
y_subset = "".join(y[i] for i in good_positions)
result[k] = y_subset
if args.output_filename:
with open(args.output_filename, 'w') as f:
for k,v in result.iteritems():
f.write(">%s\n%s\n" % (k, v))
def RemapReadsSingle(self, count=None):
#scores = {}
counter = 0
has_score = False
write_mode = 'wb'
if self.binary_mode is False:
write_mode = 'wh'
self._out = pysam.Samfile(self.sam_out,
mode=write_mode,
referencenames=self.sam_in.references,
referencelengths=self.sam_in.lengths,
header=self._MakeHeader(self.sam_in.header)
)
for read in self.sam_in.fetch():
'Optional setting of count, to only realign count reads'
if count is not None and counter > count:
break
if read.is_unmapped is True:
self._out.write(read)
continue
if counter == 0:
# Check if an alignment score is already present
# If it is then record this in the has_score flag
tags = read.tags
for i in range(0, len(tags)):
if tags[i][0] == 'AS':
has_score = True
continue
if self.only_gapped is True:
has_indel = False
for c in read.cigar:
if c[0] == 1 or c[0] == 2:
# read has an indel
has_indel = True
break
if has_indel == False:
# Read must not have an indel
print(read.qname + ', ' + str(read.cigar) + " does not have an indel")
self._out.write(read)
continue
'''
if the read is a perfect match then don't realign
'''
fivep_soft_clip = 0
threep_soft_clip = 0
cigar_last = len(read.cigar) - 1
if read.cigar[0][0] == 4:
fivep_soft_clip = read.cigar[0][1]
if read.cigar[cigar_last][0] == 4:
threep_soft_clip = read.cigar[cigar_last][1]
ref = self.ref.fetch(reference=self.refnames[read.tid],
start=read.aend - read.alen - fivep_soft_clip,
end=read.aend + threep_soft_clip)
# Realign sense strand reads
query = ''
subject = ''
if self.reverse_sense is True and read.is_reverse is False:
query = self.ReverseSeq(read.seq)
subject = self.ReverseSeq(ref.upper())
#query = Seq(read.seq).complement().tostring()
#subject = Seq(ref.upper()).complement().tostring()
else:
query = read.seq
subject = ref.upper()
aln = nw.global_align(query, subject,
gap_open=self.gap_open,
gap_extend=self.gap_extend,
matrix=self.matrix)
if self.compute_scores is True:
score = nw.score_alignment(aln[0], aln[1],
gap_open=self.gap_open,
gap_extend=self.gap_extend,
matrix=self.matrix)
if has_score is True:
as_index = None
tags = read.tags
for i in range(0, len(tags)):
if tags[i][0] == 'AS':
as_index = i
if as_index is None:
raise ValueError("Read " + read.qname +
" is missing an alignment score.")
tags[as_index] = ('AS', score)
read.tags = tags
else:
read.tags = [('AS', score)] + read.tags
bam_cigar = self._MakeBamCigar(aln, read)
if self.reverse_sense is True and read.is_reverse is False:
bam_cigar.reverse()
if self.verbose is True:
self.PrettyPrint(read, aln, bam_cigar)
# New read
read.cigar = bam_cigar
read.tags = read.tags + [('OC', self._MakeSamCigar(read.cigar)),
('OP', read.pos)]
self._out.write(read)
counter += 1
self._out.close
def nwalign(s1,s2):
return nw.global_align(s1,s2)
def get_rmsd_rotation_and_translations(file1, file2):
nres1 = get_total_residue_number(file1)
nres2 = get_total_residue_number(file2)
seq1, ind1 = get_sequence_from_PDB(file1)
seq2, ind2 = get_sequence_from_PDB(file2)
alignment = nw.global_align(seq1, seq2)
nalign = len(alignment[0])
nresidues_min = min(len(seq1), len(seq2))
ind1new = []
ind2new = []
idx1, idx2 = 0, 0
for idx in range(nalign):
if (idx <
nresidues_min) and seq1[idx] == seq2[idx] and seq1[idx] != '-':
ind1new.append(ind1[idx1])
ind2new.append(ind2[idx2])
if (idx < len(seq1)) and seq1[idx] != '-':
idx1 += 1
if (idx < len(seq2)) and seq2[idx] != '-':
idx2 += 1
ind1 = ind1new
ind2 = ind2new
#TODO: add a threshold for the number of residues considered
frac1 = len(ind1) * 100.0 / nres1
frac2 = len(ind2) * 100.0 / nres2
# get coordinates of specific residues
coords1, ind1 = get_residues_coordinates(file1, ind1)
coords2, ind2 = get_residues_coordinates(file2, ind2)
new_coords1 = []
new_coords2 = []
# check if there is consistency in atom names
nresidues1 = len(coords1)
for idx in range(nresidues1):
coords1_res = coords1[idx]
coords2_res = coords2[idx]
atomnames1 = [item[0] for item in coords1_res]
atomnames2 = [item[0] for item in coords2_res]
if set(atomnames1) != set(atomnames2):
sys.exit(
"Inconsistency found in residue %s in file %s and residue %s in file %s! Missing atom suspected..."
% (ind1[idx], file1, ind2[idx], file2))
# create new coordinates
for an1, x1, y1, z1 in coords1_res:
for an2, x2, y2, z2 in coords2_res:
if an1 == an2:
new_coords1.append([x1, y1, z1])
new_coords2.append([x2, y2, z2])
break
new_coords1 = np.array(new_coords1).T
new_coords2 = np.array(new_coords2).T
rotation = np.zeros(9)
trans1 = -new_coords1[:, 0]
trans2 = -new_coords2[:, 0]
rmsd = pyqcprot.CalcRMSDRotationalMatrix(new_coords1, new_coords2,
rotation, None)
rotation = rotation.reshape((3, 3))
trans1 += new_coords1[:, 0]
trans2 += new_coords2[:, 0]
return rotation, trans1, trans2
idB = record.id
seqB = str(record.seq)
bSeqs[idB] = seqB
handle.close()
handle = open(fileNameToAlign, "rU")
for line in handle:
line = line.rstrip()
genesList = line.split("\t")
geneA = genesList[0]
geneB = genesList[1]
seqTxtA = aSeqs[geneA].upper()
seqTxtB = bSeqs[geneB].upper()
alignment = nwalign.global_align(seqTxtA, seqTxtB, matrix='NUC.4.4',gap_open=-11,gap_extend=-1)
lenOfSeqs = len(alignment[0])
matched = 0
for i in range(len(alignment[0])):
currLetterA = alignment[0][i]
currLetterB = alignment[1][i]
print currLetterA
print currLetterB
if currLetterA == currLetterB:
matched = matched + 1;
print str(matched)
print str(lenOfSeqs)
norm = float(d[i-1][j-1]/((len(s1)+len(s2))/2.0))
return norm
# 1
if sys.argv[1] == "1":
print "Pregunta 1..."
sec_ini = generar_secuencia()
# d, d_matrix, sec_mut = [], [], []
d, sec_mut = [], []
sec_mut = sec_ini
tmp1 = "".join(sec_ini)
for i in range(m+1):
sec_mut = mutacion(sec_mut,i)
tmp2 = "".join(sec_mut)
align = nw.global_align(tmp1,tmp2)
distance =distancia(align[0],align[1])
# matrix,distance =distancia(align[0],align[1])
d.append(distance)
# d_matrix.append(matrix)
tmp2 = ""
p.show()
plot1 = p.plot(m_list,d)
title(u'Distancia vs Número de mutaciones')
grid(True)
p.axis([0,m,0,max(d)+1],0.01)
xlabel(u'Número de mutaciones')
ylabel('Distancia')
p.show()
