def __init__(self, dm, config, name):
self.omConf = {}
for k in ['parentDevice', 'transform']:
if k in config:
self.omConf[k] = config.pop(k)
OptomechDevice.__init__(self, dm, config, name)
DAQGeneric.__init__(self, dm, config, name)
def __init__(self, manager, config, name):
self.config = config
self.manager = manager
self.hasPowerIndicator = False
self.hasShutter = False
self.hasTriggerableShutter = False
self.hasQSwitch = False
self.hasPCell = False
self.hasTunableWavelength = False
self.params = {
'expectedPower': config.get('power', None), ## Expected output
'currentPower': None, ## Last measured output power
'scopeTransmission':
None, ## percentage of output power that is transmitted to slice
'tolerance': 10.0, ## in %
'useExpectedPower': True,
'powerAlert':
True ## set True if you want changes in output power to raise an error
}
daqConfig = {
} ### DAQ generic needs to know about powerIndicator, pCell, shutter, qswitch
if 'powerIndicator' in config:
self.hasPowerIndicator = True
#### name of powerIndicator is config['powerIndicator']['channel'][0]
#daqConfig['powerInd'] = {'channel': config['powerIndicator']['channel'], 'type': 'ai'}
if 'shutter' in config:
daqConfig['shutter'] = config['shutter']
self.hasTriggerableShutter = True
self.hasShutter = True
if 'qSwitch' in config:
daqConfig['qSwitch'] = config['qSwitch']
self.hasQSwitch = True
if 'pCell' in config:
daqConfig['pCell'] = config['pCell']
self.hasPCell = True
daqConfig['power'] = {
'type': 'ao',
'units': 'W'
} ## virtual channel used for creating control widgets
DAQGeneric.__init__(self, manager, daqConfig, name)
OptomechDevice.__init__(self, manager, config, name)
self.lock = Mutex(QtCore.QMutex.Recursive)
self.variableLock = Mutex(QtCore.QMutex.Recursive)
self.calibrationIndex = None
self.pCellCalibration = None
self.getPowerHistory()
#self.scope = manager.getDevice(self.config['scope'])
#self.scope.sigObjectiveChanged.connect(self.objectiveChanged)
self.sigGlobalSubdeviceChanged.connect(
self.opticStateChanged
) ## called when objectives/filters have been switched
manager.declareInterface(name, ['laser'], self)
manager.sigAbortAll.connect(self.closeShutter)
def quit(self):
#print "quit() called from Camera"
if hasattr(self, 'acqThread') and self.isRunning():
self.stop()
if not self.wait(10000):
raise Exception("Timed out while waiting for thread exit!")
#self.cam.close()
DAQGeneric.quit(self)
def quit(self):
#print "quit() called from Camera"
if hasattr(self, 'acqThread') and self.isRunning():
self.stop()
if not self.wait(10000):
raise Exception("Timed out while waiting for thread exit!")
#self.cam.close()
DAQGeneric.quit(self)
def __init__(self, manager, config, name):
self.config = config
self.manager = manager
self.hasPowerIndicator = False
self.hasShutter = False
self.hasTriggerableShutter = False
self.hasQSwitch = False
self.hasPCell = False
self.hasTunableWavelength = False
self.params = {
'expectedPower': config.get('power', None), ## Expected output
'currentPower': None, ## Last measured output power
'scopeTransmission': None, ## percentage of output power that is transmitted to slice
'tolerance': 10.0, ## in %
'useExpectedPower': True,
'powerAlert': True ## set True if you want changes in output power to raise an error
}
daqConfig = {} ### DAQ generic needs to know about powerIndicator, pCell, shutter, qswitch
if 'powerIndicator' in config:
self.hasPowerIndicator = True
#### name of powerIndicator is config['powerIndicator']['channel'][0]
#daqConfig['powerInd'] = {'channel': config['powerIndicator']['channel'], 'type': 'ai'}
if 'shutter' in config:
daqConfig['shutter'] = config['shutter']
self.hasTriggerableShutter = True
self.hasShutter = True
if 'qSwitch' in config:
daqConfig['qSwitch'] = config['qSwitch']
self.hasQSwitch = True
if 'pCell' in config:
daqConfig['pCell'] = config['pCell']
self.hasPCell = True
daqConfig['power'] = {'type': 'ao', 'units': 'W'} ## virtual channel used for creating control widgets
DAQGeneric.__init__(self, manager, daqConfig, name)
OptomechDevice.__init__(self, manager, config, name)
self._configDir = os.path.join('devices', self.name() + '_config')
self.lock = Mutex(QtCore.QMutex.Recursive)
self.variableLock = Mutex(QtCore.QMutex.Recursive)
self.calibrationIndex = None
self.pCellCalibration = None
self.getPowerHistory()
#self.scope = manager.getDevice(self.config['scope'])
#self.scope.sigObjectiveChanged.connect(self.objectiveChanged)
self.sigGlobalSubdeviceChanged.connect(self.opticStateChanged) ## called when objectives/filters have been switched
manager.declareInterface(name, ['laser'], self)
def getDAQName(self, channel=None):
if channel is None:
if self.hasTriggerableShutter:
ch = 'shutter'
daqName = DAQGeneric.getDAQName(self, 'shutter')
elif self.hasPCell:
ch = 'pCell'
daqName = DAQGeneric.getDAQName(self, 'pCell')
elif self.hasQSwitch:
ch = 'qSwitch'
daqName = DAQGeneric.getDAQName(self, 'qSwitch')
else:
return (None, None)
#raise HelpfulException("LaserTask can't find name of DAQ device to use for this task.")
return (daqName, ch)
else:
return DAQGeneric.getDAQName(self, channel)
def getDAQName(self, channel=None):
if channel is None:
if self.hasTriggerableShutter:
ch = 'shutter'
daqName = DAQGeneric.getDAQName(self, 'shutter')
elif self.hasPCell:
ch = 'pCell'
daqName = DAQGeneric.getDAQName(self, 'pCell')
elif self.hasQSwitch:
ch = 'qSwitch'
daqName = DAQGeneric.getDAQName(self, 'qSwitch')
else:
return (None, None)
#raise HelpfulException("LaserTask can't find name of DAQ device to use for this task.")
return (daqName, ch)
else:
return DAQGeneric.getDAQName(self, channel)
def setHolding(self, mode=None, value=None, force=False):
# print "setHolding", mode, value
global ivModes
with self.devLock:
currentMode = self.getMode()
if mode is None:
mode = currentMode
ivMode = ivModes[mode] ## determine vc/ic
if value is None:
value = self.holding[ivMode]
else:
self.holding[ivMode] = value
if ivMode == ivModes[currentMode] or force:
mapping = self.getMapping(mode=mode)
## override the scale since getChanScale won't necessarily give the correct value
## (we may be about to switch modes)
DAQGeneric.setChanHolding(self, "command", value, mapping=mapping)
self.sigHoldingChanged.emit("primary", self.holding.copy())
def setHolding(self, mode=None, value=None, force=False):
#print "setHolding", mode, value
#global ivModes
with self.devLock:
currentMode = self.getMode()
if mode is None:
mode = currentMode
ivMode = self.ivModes[mode] ## determine vc/ic
if value is None:
value = self.holding[ivMode]
else:
self.holding[ivMode] = value
if ivMode == self.ivModes[currentMode] or force:
mapping = self.getMapping(mode=mode)
## override the scale since getChanScale won't necessarily give the correct value
## (we may be about to switch modes)
DAQGeneric.setChanHolding(self, 'command', value, mapping=mapping)
self.sigHoldingChanged.emit('primary', self.holding.copy())
def __init__(self, dm, config, name):
# Generate config to use for DAQ
self.devLock = Mutex(Mutex.Recursive)
daqConfig = {
'command': config['Command'],
'primary': config['ScaledSignal'],
}
self.holding = {
'vc': config.get('vcHolding', -0.05),
'ic': config.get('icHolding', 0.0)
}
self.mode = 'i=0'
self.config = config
DAQGeneric.__init__(self, dm, daqConfig, name)
try:
self.setHolding()
except:
printExc("Error while setting holding value:")
# Start a remote process to run the simulation.
self.process = mp.Process()
rsys = self.process._import('sys')
rsys._setProxyOptions(
returnType='proxy'
) # need to access remote path by proxy, not by value
rsys.path.append(os.path.abspath(os.path.dirname(__file__)))
if config['simulator'] == 'builtin':
self.simulator = self.process._import('hhSim')
elif config['simulator'] == 'neuron':
self.simulator = self.process._import('neuronSim')
dm.declareInterface(name, ['clamp'], self)
def __init__(self, dm, config, name):
# Generate config to use for DAQ
daqConfig = {}
self.devLock = Mutex(Mutex.Recursive)
daqConfig = {
'command': config['Command'],
'primary': config['ScaledSignal'],
}
self.holding = {
'vc': config.get('vcHolding', -0.05),
'ic': config.get('icHolding', 0.0)
}
self.mode = 'i=0'
self.config = config
DAQGeneric.__init__(self, dm, daqConfig, name)
try:
self.setHolding()
except:
printExc("Error while setting holding value:")
# Start a remote process to run the simulation.
self.process = mp.Process()
rsys = self.process._import('sys')
rsys._setProxyOptions(returnType='proxy') # need to access remote path by proxy, not by value
rsys.path.append(os.path.abspath(os.path.dirname(__file__)))
if config['simulator'] == 'builtin':
self.simulator = self.process._import('hhSim')
elif config['simulator'] == 'neuron':
self.simulator = self.process._import('neuronSima')
dm.declareInterface(name, ['clamp'], self)
def setChanHolding(self, chan, value=None):
if chan == 'command':
self.setHolding(value=value)
else:
DAQGeneric.setChanHolding(self, chan, value)
def quit(self):
#self.process.send(None)
self.process.close()
DAQGeneric.quit(self)
def getChanHolding(self, chan):
if chan == 'command':
return self.getHolding()
else:
return DAQGeneric.getChanHolding(self, chan)
def __init__(self, dm, config, name):
# Generate config to use for DAQ
daqConfig = {}
for ch in ['GainChannel', 'LPFChannel', 'ModeChannel']:
if ch not in config:
continue
daqConfig[ch] = config[ch].copy()
#if 'GainChannel' in config:
# daqConfig['gain'] = {'type': 'ai', 'channel': config['GainChannel']}
#if 'LPFChannel' in config:
# daqConfig['LPF'] = {'type': 'ai', 'channel': config['LPFChannel'], 'units': 'Hz'}
if 'ScaledSignal' in config:
#daqConfig['primary'] = {'type': 'ai', 'channel': config['ScaledSignal']}
daqConfig['primary'] = config['ScaledSignal']
if config['ScaledSignal'].get('type', None) != 'ai':
raise Exception("AxoPatch200: ScaledSignal configuration must have type:'ai'")
if 'Command' in config:
#daqConfig['command'] = {'type': 'ao', 'channel': config['Command']}
daqConfig['command'] = config['Command']
if config['Command'].get('type', None) != 'ao':
raise Exception("AxoPatch200: ScaledSignal configuration must have type:'ao'")
## Note that both of these channels can be present, but we will only ever record from one at a time.
## Usually, we'll record from "I OUTPUT" in current clamp and "10 Vm OUTPUT" in voltage clamp.
if 'SecondaryVCSignal' in config:
self.hasSecondaryChannel = True
#daqConfig['secondary'] = {'type': 'ai', 'channel': config['SecondaryVCSignal']}
daqConfig['secondary'] = config['SecondaryVCSignal']
if config['SecondaryVCSignal'].get('type', None) != 'ai':
raise Exception("AxoPatch200: SecondaryVCSignal configuration must have type:'ai'")
elif 'SecondaryICSignal' in config:
self.hasSecondaryChannel = True
#daqConfig['secondary'] = {'type': 'ai', 'channel': config['SecondaryICSignal']}
daqConfig['secondary'] = config['SecondaryICSignal']
if config['SecondaryICSignal'].get('type', None) != 'ai':
raise Exception("AxoPatch200: SecondaryICSignal configuration must have type:'ai'")
else:
self.hasSecondaryChannel = False
self.version = config.get('version', '200B')
# Axopatch gain telegraph
# telegraph should not read below 2 V in CC mode
self.gain_tel = np.array([0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5])
self.gain_vm = np.array([0.5, 0.5, 0.5, 0.5, 1, 2, 5, 10, 20, 50, 100, 200, 500]) * 1e9 ## values in mv/pA
self.gain_im = np.array([0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100, 200, 500]) ## values in mV/mV
# Axopatch Lowpass Bessel Filter
self.lpf_tel = np.array([2.0, 4.0, 6.0, 8.0, 10.0])
self.lpf_freq = np.array([1.0, 2.0, 5.0, 10.0, 50.0])
if self.version == '200':
# telegraph voltage/output translation from the Axopatch 200 amplifier
self.mode_tel = np.array([6, 4, 2])
self.modeNames = OrderedDict([(0, 'V-Clamp'), (1, 'Track'), (2, 'I-Clamp')])
self.ivModes = {'V-Clamp':'vc', 'Track':'ic', 'I-Clamp':'ic', 'vc':'vc', 'ic':'ic'}
self.modeAliases = {'ic': 'I-Clamp', 'i=0': 'Track', 'vc': 'V-Clamp'}
elif self.version == '200A':
# telegraph voltage/output translation from the Axopatch 200 amplifier
self.mode_tel = np.array([6, 4, 2, 1])
self.modeNames = OrderedDict([(0, 'V-Clamp'), (1, 'Track'), (2, 'I-Clamp Normal'), (3, 'I-Clamp Fast'), ])
self.ivModes = {'V-Clamp':'vc', 'Track':'vc', 'I-Clamp Fast':'ic', 'I-Clamp Normal':'ic', 'vc':'vc', 'ic':'ic'}
self.modeAliases = {'ic': 'I-Clamp Fast', 'i=0': 'Track', 'vc': 'V-Clamp'}
elif self.version == '200B':
# telegraph voltage/output translation from the Axopatch 200 amplifier
self.mode_tel = np.array([6, 4, 3, 2, 1])
self.modeNames = OrderedDict([(0, 'V-Clamp'), (2, 'I=0'), (4, 'I-Clamp Fast'), (3, 'I-Clamp Normal'), (1, 'Track'), ])
self.ivModes = {'V-Clamp':'vc', 'Track':'vc', 'I=0':'ic', 'I-Clamp Fast':'ic', 'I-Clamp Normal':'ic', 'vc':'vc', 'ic':'ic'}
self.modeAliases = {'ic': 'I-Clamp Fast', 'i=0': 'I=0', 'vc': 'V-Clamp'}
self.lpf_freq[-1] = 100.0 # 200B's highest LPF value is 100kHz instead of 50.
else:
raise Exception("AxoPatch200: version must be '200', '200A' or '200B' (got %r)" % version)
self.holding = {
'vc': config.get('vcHolding', -0.05),
'ic': config.get('icHolding', 0.0)
}
self.config = config
self.modeLock = Mutex(Mutex.Recursive) ## protects self.mdCanceled
self.devLock = Mutex(Mutex.Recursive) ## protects self.holding, possibly self.config, ..others, perhaps?
self.mdCanceled = False
DAQGeneric.__init__(self, dm, daqConfig, name)
self.modeDialog = QtGui.QMessageBox()
self.modeDialog.hide()
self.modeDialog.setModal(False)
self.modeDialog.setWindowTitle("Mode Switch Request")
self.modeDialog.addButton(self.modeDialog.Cancel)
self.modeDialog.buttonClicked.connect(self.modeDialogClicked)
self.sigShowModeDialog.connect(self.showModeDialog)
self.sigHideModeDialog.connect(self.hideModeDialog)
try:
self.setHolding()
except:
printExc("Error while setting holding value:")
dm.declareInterface(name, ['clamp'], self)
def __init__(self, dm, config, name):
OptomechDevice.__init__(self, dm, config, name)
self.lock = Mutex(Mutex.Recursive)
# Generate config to use for DAQ
daqConfig = {}
if 'exposeChannel' in config:
daqConfig['exposure'] = config['exposeChannel']
if 'triggerInChannel' in config:
daqConfig['trigger'] = config['triggerInChannel']
DAQGeneric.__init__(self, dm, daqConfig, name)
self.camConfig = config
self.stateStack = []
if 'scaleFactor' not in self.camConfig:
self.camConfig['scaleFactor'] = [1., 1.]
## Default values for scope state. These will be used if there is no scope defined.
self.scopeState = {
'id': 0,
}
self.scopeDev = None
p = self
while p is not None:
p = p.parentDevice()
if isinstance(p, Microscope):
self.scopeDev = p
self.scopeDev.sigObjectiveChanged.connect(
self.objectiveChanged)
break
self.transformChanged()
if self.scopeDev is not None:
self.objectiveChanged()
self.setupCamera()
#print "Camera: setupCamera returned, about to create acqThread"
self.sensorSize = self.getParam('sensorSize')
tr = pg.SRTTransform3D()
tr.translate(-self.sensorSize[0] * 0.5, -self.sensorSize[1] * 0.5)
self.setDeviceTransform(self.deviceTransform() * tr)
self.acqThread = AcquireThread(self)
#print "Camera: acqThread created, about to connect signals."
self.acqThread.finished.connect(self.acqThreadFinished)
self.acqThread.started.connect(self.acqThreadStarted)
self.acqThread.sigShowMessage.connect(self.showMessage)
self.acqThread.sigNewFrame.connect(self.newFrame)
#print "Camera: signals connected:"
self.sigGlobalTransformChanged.connect(self.transformChanged)
if config != None:
# look for 'defaults', then 'params' to preserve backward compatibility.
defaults = config.get('defaults', config.get('params', {}))
#print "Camera: setting configuration params."
try:
self.setParams(defaults)
except:
printExc("Error default setting camera parameters:")
#print "Camera: no config params to set."
dm.declareInterface(name, ['camera'], self)
def __init__(self, dm, config, name):
OptomechDevice.__init__(self, dm, config, name)
self.lock = Mutex(Mutex.Recursive)
# Generate config to use for DAQ
daqConfig = {}
if 'exposeChannel' in config:
daqConfig['exposure'] = config['exposeChannel']
if 'triggerInChannel' in config:
daqConfig['trigger'] = config['triggerInChannel']
DAQGeneric.__init__(self, dm, daqConfig, name)
self.camConfig = config
self.stateStack = []
if 'scaleFactor' not in self.camConfig:
self.camConfig['scaleFactor'] = [1., 1.]
## Default values for scope state. These will be used if there is no scope defined.
self.scopeState = {
'id': 0,
#'scopePosition': [0, 0], ## position of scope in global coords
#'centerPosition': [0, 0], ## position of objective in global coords (objective may be offset from center of scope)
#'offset': [0, 0],
#'objScale': 1,
#'pixelSize': (1, 1),
#'objective': '',
#'transform': None,
}
self.scopeDev = None
p = self
while p is not None:
p = p.parentDevice()
if isinstance(p, Microscope):
self.scopeDev = p
self.scopeDev.sigObjectiveChanged.connect(self.objectiveChanged)
break
#if 'scopeDevice' in config:
#self.scopeDev = self.dm.getDevice(config['scopeDevice'])
#self.scopeDev.sigPositionChanged.connect(self.positionChanged)
### Cache microscope state for fast access later
#self.objectiveChanged()
#self.positionChanged()
#else:
#self.scopeDev = None
self.transformChanged()
if self.scopeDev is not None:
self.objectiveChanged()
self.setupCamera()
#print "Camera: setupCamera returned, about to create acqThread"
self.sensorSize = self.getParam('sensorSize')
tr = pg.SRTTransform3D()
tr.translate(-self.sensorSize[0]*0.5, -self.sensorSize[1]*0.5)
self.setDeviceTransform(self.deviceTransform() * tr)
self.acqThread = AcquireThread(self)
#print "Camera: acqThread created, about to connect signals."
self.acqThread.finished.connect(self.acqThreadFinished)
self.acqThread.started.connect(self.acqThreadStarted)
self.acqThread.sigShowMessage.connect(self.showMessage)
self.acqThread.sigNewFrame.connect(self.newFrame)
#print "Camera: signals connected:"
self.sigGlobalTransformChanged.connect(self.transformChanged)
if config != None:
# look for 'defaults', then 'params' to preserve backward compatibility.
defaults = config.get('defaults', config.get('params', {}))
#print "Camera: setting configuration params."
try:
self.setParams(defaults)
except:
printExc("Error default setting camera parameters:")
#print "Camera: no config params to set."
dm.declareInterface(name, ['camera'], self)
def getChanHolding(self, chan):
if chan == 'command':
return self.getHolding()
else:
return DAQGeneric.getChanHolding(self, chan)
def __init__(self, dm, config, name):
# Generate config to use for DAQ
daqConfig = {}
for ch in ["GainChannel", "LPFChannel", "ModeChannel"]:
if ch not in config:
continue
daqConfig[ch] = config[ch].copy()
# if 'GainChannel' in config:
# daqConfig['gain'] = {'type': 'ai', 'channel': config['GainChannel']}
# if 'LPFChannel' in config:
# daqConfig['LPF'] = {'type': 'ai', 'channel': config['LPFChannel'], 'units': 'Hz'}
if "ScaledSignal" in config:
# daqConfig['primary'] = {'type': 'ai', 'channel': config['ScaledSignal']}
daqConfig["primary"] = config["ScaledSignal"]
if config["ScaledSignal"].get("type", None) != "ai":
raise Exception("AxoPatch200: ScaledSignal configuration must have type:'ai'")
if "Command" in config:
# daqConfig['command'] = {'type': 'ao', 'channel': config['Command']}
daqConfig["command"] = config["Command"]
if config["Command"].get("type", None) != "ao":
raise Exception("AxoPatch200: ScaledSignal configuration must have type:'ao'")
## Note that both of these channels can be present, but we will only ever record from one at a time.
## Usually, we'll record from "I OUTPUT" in current clamp and "10 Vm OUTPUT" in voltage clamp.
if "SecondaryVCSignal" in config:
self.hasSecondaryChannel = True
# daqConfig['secondary'] = {'type': 'ai', 'channel': config['SecondaryVCSignal']}
daqConfig["secondary"] = config["SecondaryVCSignal"]
if config["SecondaryVCSignal"].get("type", None) != "ai":
raise Exception("AxoPatch200: SecondaryVCSignal configuration must have type:'ai'")
elif "SecondaryICSignal" in config:
self.hasSecondaryChannel = True
# daqConfig['secondary'] = {'type': 'ai', 'channel': config['SecondaryICSignal']}
daqConfig["secondary"] = config["SecondaryICSignal"]
if config["SecondaryICSignal"].get("type", None) != "ai":
raise Exception("AxoPatch200: SecondaryICSignal configuration must have type:'ai'")
else:
self.hasSecondaryChannel = False
self.holding = {"vc": config.get("vcHolding", -0.05), "ic": config.get("icHolding", 0.0)}
self.config = config
self.modeLock = Mutex(Mutex.Recursive) ## protects self.mdCanceled
self.devLock = Mutex(Mutex.Recursive) ## protects self.holding, possibly self.config, ..others, perhaps?
self.mdCanceled = False
DAQGeneric.__init__(self, dm, daqConfig, name)
self.modeDialog = QtGui.QMessageBox()
self.modeDialog.hide()
self.modeDialog.setModal(False)
self.modeDialog.setWindowTitle("Mode Switch Request")
self.modeDialog.addButton(self.modeDialog.Cancel)
self.modeDialog.buttonClicked.connect(self.modeDialogClicked)
self.sigShowModeDialog.connect(self.showModeDialog)
self.sigHideModeDialog.connect(self.hideModeDialog)
try:
self.setHolding()
except:
printExc("Error while setting holding value:")
dm.declareInterface(name, ["clamp"], self)
def quit(self):
#self.process.send(None)
self.process.close()
DAQGeneric.quit(self)
def setChanHolding(self, chan, value=None):
if chan == 'command':
self.setHolding(value=value)
else:
DAQGeneric.setChanHolding(self, chan, value)
