Example #1
0
 def _populate_iso(self, iso, layout):
     StockSampleCreationIsoPopulator._populate_iso(self, iso, layout)
     # Create sector preparation plates.
     library_name = self.iso_request.label
     ir_specs_96 = get_reservoir_specs_standard_96()
     plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96)
     ir_specs_384 = get_reservoir_specs_standard_384()
     plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384)
     future_status = get_item_status_future()
     sec_layout_map = get_sector_layouts_for_384_layout(layout)
     # Create preparation plates.
     for sec_idx in range(NUMBER_SECTORS):
         if not sec_idx in sec_layout_map:
             continue
         # TODO: Move label creation to LABELS class.
         prep_label = self.PREP_PLATE_LABEL_PATTERN \
                             % (library_name,
                                iso.layout_number,
                                DEFAULT_PREPARATION_PLATE_CONCENTRATION,
                                sec_idx + 1)
         prep_plate = plate_specs_96.create_rack(prep_label, future_status)
         sec_layout = sec_layout_map[sec_idx]
         iso.add_sector_preparation_plate(prep_plate, sec_idx,
                                          sec_layout.create_rack_layout())
     # Create aliquot plates.
     for i in range(self.iso_request.number_aliquots):
         # TODO: Move label creation to LABELS class.
         aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN \
                             % (library_name,
                                iso.layout_number,
                                DEFAULT_ALIQUOT_PLATE_CONCENTRATION,
                                i + 1)
         aliquot_plate = plate_specs_384.create_rack(aliquot_label,
                                                     future_status)
         iso.add_aliquot_plate(aliquot_plate)
Example #2
0
    def __populate_isos(self):
        """
        Adds molecule design set, library layout and plates to the picked ISOs.
        """
        self.add_debug('Create ISOs ...')

        ir_specs_96 = get_reservoir_specs_standard_96()
        plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96)
        ir_specs_384 = get_reservoir_specs_standard_384()
        plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384)
        future_status = get_item_status_future()
        library_name = self._iso_request.plate_set_label
        md_type = \
          self.molecule_design_library.molecule_design_pool_set.molecule_type

        while len(self.__picked_isos) > 0:
            lci = self.__picked_isos.pop(0)
            library_layout = self._library_layouts.pop(0)
            lci.rack_layout = library_layout.create_rack_layout()
            lci.molecule_design_pool_set = \
                        library_layout.get_pool_set(md_type)
            layout_number = lci.layout_number

            # create source plates
            for sector_index in self._quadrant_positions.keys():
                prep_label = self.PREP_PLATE_LABEL_PATTERN % (
                    library_name, layout_number,
                    PREPARATION_PLATE_CONCENTRATION, (sector_index + 1))
                prep_plate = plate_specs_96.create_rack(label=prep_label,
                                                        status=future_status)
                LibrarySourcePlate(iso=lci,
                                   plate=prep_plate,
                                   sector_index=sector_index)

            # create aliquot plates
            for i in range(STARTING_NUMBER_ALIQUOTS):
                aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN % (
                    library_name, layout_number, ALIQUOT_PLATE_CONCENTRATION,
                    (i + 1))
                aliquot_plate = plate_specs_384.create_rack(
                    label=aliquot_label, status=future_status)
                IsoAliquotPlate(iso=lci, plate=aliquot_plate)

            self.__new_isos.append(lci)
Example #3
0
    def __populate_isos(self):
        """
        Adds molecule design set, library layout and plates to the picked ISOs.
        """
        self.add_debug('Create ISOs ...')

        ir_specs_96 = get_reservoir_specs_standard_96()
        plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96)
        ir_specs_384 = get_reservoir_specs_standard_384()
        plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384)
        future_status = get_item_status_future()
        library_name = self._iso_request.plate_set_label
        md_type = \
          self.molecule_design_library.molecule_design_pool_set.molecule_type

        while len(self.__picked_isos) > 0:
            lci = self.__picked_isos.pop(0)
            library_layout = self._library_layouts.pop(0)
            lci.rack_layout = library_layout.create_rack_layout()
            lci.molecule_design_pool_set = \
                        library_layout.get_pool_set(md_type)
            layout_number = lci.layout_number

            # create source plates
            for sector_index in self._quadrant_positions.keys():
                prep_label = self.PREP_PLATE_LABEL_PATTERN % (library_name,
                                layout_number, PREPARATION_PLATE_CONCENTRATION,
                                (sector_index + 1))
                prep_plate = plate_specs_96.create_rack(label=prep_label,
                                                        status=future_status)
                LibrarySourcePlate(iso=lci, plate=prep_plate,
                                   sector_index=sector_index)

            # create aliquot plates
            for i in range(STARTING_NUMBER_ALIQUOTS):
                aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN % (
                                library_name, layout_number,
                                ALIQUOT_PLATE_CONCENTRATION, (i + 1))
                aliquot_plate = plate_specs_384.create_rack(label=aliquot_label,
                                                        status=future_status)
                IsoAliquotPlate(iso=lci, plate=aliquot_plate)

            self.__new_isos.append(lci)