def _populate_iso(self, iso, layout): StockSampleCreationIsoPopulator._populate_iso(self, iso, layout) # Create sector preparation plates. library_name = self.iso_request.label ir_specs_96 = get_reservoir_specs_standard_96() plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96) ir_specs_384 = get_reservoir_specs_standard_384() plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384) future_status = get_item_status_future() sec_layout_map = get_sector_layouts_for_384_layout(layout) # Create preparation plates. for sec_idx in range(NUMBER_SECTORS): if not sec_idx in sec_layout_map: continue # TODO: Move label creation to LABELS class. prep_label = self.PREP_PLATE_LABEL_PATTERN \ % (library_name, iso.layout_number, DEFAULT_PREPARATION_PLATE_CONCENTRATION, sec_idx + 1) prep_plate = plate_specs_96.create_rack(prep_label, future_status) sec_layout = sec_layout_map[sec_idx] iso.add_sector_preparation_plate(prep_plate, sec_idx, sec_layout.create_rack_layout()) # Create aliquot plates. for i in range(self.iso_request.number_aliquots): # TODO: Move label creation to LABELS class. aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN \ % (library_name, iso.layout_number, DEFAULT_ALIQUOT_PLATE_CONCENTRATION, i + 1) aliquot_plate = plate_specs_384.create_rack(aliquot_label, future_status) iso.add_aliquot_plate(aliquot_plate)
def __populate_isos(self): """ Adds molecule design set, library layout and plates to the picked ISOs. """ self.add_debug('Create ISOs ...') ir_specs_96 = get_reservoir_specs_standard_96() plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96) ir_specs_384 = get_reservoir_specs_standard_384() plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384) future_status = get_item_status_future() library_name = self._iso_request.plate_set_label md_type = \ self.molecule_design_library.molecule_design_pool_set.molecule_type while len(self.__picked_isos) > 0: lci = self.__picked_isos.pop(0) library_layout = self._library_layouts.pop(0) lci.rack_layout = library_layout.create_rack_layout() lci.molecule_design_pool_set = \ library_layout.get_pool_set(md_type) layout_number = lci.layout_number # create source plates for sector_index in self._quadrant_positions.keys(): prep_label = self.PREP_PLATE_LABEL_PATTERN % ( library_name, layout_number, PREPARATION_PLATE_CONCENTRATION, (sector_index + 1)) prep_plate = plate_specs_96.create_rack(label=prep_label, status=future_status) LibrarySourcePlate(iso=lci, plate=prep_plate, sector_index=sector_index) # create aliquot plates for i in range(STARTING_NUMBER_ALIQUOTS): aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN % ( library_name, layout_number, ALIQUOT_PLATE_CONCENTRATION, (i + 1)) aliquot_plate = plate_specs_384.create_rack( label=aliquot_label, status=future_status) IsoAliquotPlate(iso=lci, plate=aliquot_plate) self.__new_isos.append(lci)
def __populate_isos(self): """ Adds molecule design set, library layout and plates to the picked ISOs. """ self.add_debug('Create ISOs ...') ir_specs_96 = get_reservoir_specs_standard_96() plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96) ir_specs_384 = get_reservoir_specs_standard_384() plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384) future_status = get_item_status_future() library_name = self._iso_request.plate_set_label md_type = \ self.molecule_design_library.molecule_design_pool_set.molecule_type while len(self.__picked_isos) > 0: lci = self.__picked_isos.pop(0) library_layout = self._library_layouts.pop(0) lci.rack_layout = library_layout.create_rack_layout() lci.molecule_design_pool_set = \ library_layout.get_pool_set(md_type) layout_number = lci.layout_number # create source plates for sector_index in self._quadrant_positions.keys(): prep_label = self.PREP_PLATE_LABEL_PATTERN % (library_name, layout_number, PREPARATION_PLATE_CONCENTRATION, (sector_index + 1)) prep_plate = plate_specs_96.create_rack(label=prep_label, status=future_status) LibrarySourcePlate(iso=lci, plate=prep_plate, sector_index=sector_index) # create aliquot plates for i in range(STARTING_NUMBER_ALIQUOTS): aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN % ( library_name, layout_number, ALIQUOT_PLATE_CONCENTRATION, (i + 1)) aliquot_plate = plate_specs_384.create_rack(label=aliquot_label, status=future_status) IsoAliquotPlate(iso=lci, plate=aliquot_plate) self.__new_isos.append(lci)